120 results about "Immunogenic cell death" patented technology

The invention discloses an engineered autologous tumor tissue scaffold and application thereof, and the engineered autologous tumor tissue scaffold is prepared by performing specific treatment on autologous tumor tissue to induce immunogenic cell death of the tumor tissue, and then sequentially performing freezing and drying steps; the engineered autologous tumor tissue scaffold can be used for loading dendritic cells and constructing personalized tumor vaccines. According to the stent, autologous tumor tissue is adopted, so that a specific and comprehensive tumor antigen pedigree is reserved, and multi-epitope immunostimulation is provided; and meanwhile, excellent biocompatibility is ensured, the immunological rejection risk is reduced, and relatively high safety is ensured.

The invention relates to the technical field of biological medicine, in particular to immunogen cell death enhanced nano-vesicle hydrogel and application thereof. The invention provides a 3D-printable multifunctional synergistic immunogen cell death enhanced nano-vesicle hydrogel, which is characterized in that SerMA hydrogel is used as a matrix, cell membrane nano-vesicles (N (at) ILO) loaded with a photothermal agent IR1061, a chemotherapeutic drug OXA and a tumor acidic microenvironment regulator Lon are encapsulated, initial tumor immunogen cell death is triggered through a photothermal effect, and the tumor immunogen cell death enhanced nano-vesicle hydrogel can be used for 3D printing. OXA further enhances the ICD effect, Lon regulates the acidic tumor microenvironment to improve the T cell activity, and postoperative multi-mode synergistic anti-tumor treatment is achieved; meanwhile, the hydrogel also has excellent photocuring 3D printing capability, can be used for individually constructing a filling structure matched with a postoperative defect area, has a wide application prospect, and provides a new strategy and theoretical basis for postoperative comprehensive treatment of choroidal melanoma.

The invention relates to the technical field of antitumor drugs, in particular to a Ru (II) complex and a preparation method and application thereof, and the Ru (II) complex has a structure as shown in a formula I. The Ru (II) complex synthesized by the method disclosed by the invention not only has relatively high cytotoxicity, but also has good photodynamic antitumor activity. After the complex is illuminated, the efficiency of the complex entering cells in an active transportation mode can be accelerated, mitochondria and endoplasmic reticulum are targeted at the same time, mitochondrial membrane potential decline and endoplasmic reticulum stress are caused to form a dual organelle damage effect, immunogenic cell death is caused, the tumor microenvironment is adjusted, and the tumor cell immunogenicity is improved. The enrichment of dendritic cells (DCs) in tumor cells is realized, the chemotactic activity of effector T cells is improved, the proportion of CD4 < + > and Foxp3 < + > cell populations is reduced, and finally the anti-tumor immune response is activated. Meanwhile, the complex provided by the invention can also induce the cell to generate pan apoptosis, retard the cell cycle in the G2 / M period and enhance the effect of the complex in inhibiting tumor proliferation.

The invention relates to the technical field of medicines, in particular to a composite nano vaccine and application thereof. The composite nano vaccine is prepared by coupling a natural compound with endoplasmic reticulum stress induction capability on the surface of Ga-MOF nano particles. The composite nano vaccine can significantly enhance the anti-tumor effect through a ferroptosis mechanism, an endoplasmic reticulum stress and immunogenic cell death mechanism and a cryoablation technology synergistic effect mechanism, reverses an immunosuppressed tumor microenvironment, inhibits the growth of primary tumors and distant metastatic tumors, and has a good anti-tumor effect. A brand new strategy is provided for comprehensive treatment of tumors, and the application prospect is wide.

The invention discloses a polyethylene glycol modified gold and manganese dioxide nano particle and a preparation method and application thereof, and belongs to the technical field of biological medicine, the polyethylene glycol modified gold and manganese dioxide nano particle (GMCN and PEG) has good biocompatibility under neutral physiological conditions, can relieve tumor hypoxia and increase generation of active oxygen in acidic TME, and has a good anti-tumor effect. The radiotherapy sensitivity is improved; and immune cell death is induced. Meanwhile, a cGAS-STING signal channel can be activated, dendritic cell maturation, macrophage M1 polarization and T cell infiltration are promoted, and the immunosuppression state in TME is counteracted. In addition, the GMCN-coated PEG also has an MR-CT bimodal imaging enhancement function, and integration of diagnosis and treatment is realized. The nano sensitizer disclosed by the invention has huge potential in the aspects of improving the radiotherapy curative effect, remodeling the tumor microenvironment, promoting the anti-tumor immunity, enhancing the biomedical imaging and the like.

The invention relates to the technical field of hepatocellular carcinoma, in particular to a hepatocellular carcinoma prognosis model construction method based on immunogen cell death related genes and application, and an accurate prognosis prediction model is constructed by integrating the immunogen cell death related genes (IRGs) and molecular characteristics of hepatocellular carcinoma. A training set and a verification set provided by TCGA and ICGC databases are utilized, so that the model can perform effective sample analysis under a large-scale data background. Through consistency clustering analysis, the optimal clustering number is determined, the samples are orderly divided into different molecular subtypes, and it is ensured that the samples in each subtype have similar molecular characteristics. According to the invention, the capability of capturing liver cancer heterogeneity on the molecular level of the model is increased, so that the accuracy of prognosis prediction is improved.

The invention discloses an aptamer-modified lipid nano delivery platform as well as a preparation method and application thereof, and belongs to the field of biomedicine. The platform is prepared by taking DPPC, DOTAP and cholesterol as basic lipid components, Ce6 as a sound-sensitive agent and Flt3L as an immune agonist, controlling the particle size through gradient extrusion, and coupling cholesterol with an EpCAM aptamer for surface modification. The method has the core advantages that active targeting enrichment of tumors is realized by virtue of the aptamer, tumor cell immunogen cell death (ICD) is induced in combination with a sonodynamic therapy (SDT), and damage-related molecular patterns (DAMPs) are released; meanwhile, Flt3L is released in a tumor microenvironment, collection and activation of type 1 classical dendritic cells (cDC1) are specifically promoted, an'endogenous cDC1 vaccine 'is constructed, and CD8 + T cell mediated anti-tumor immune response is enhanced. Experiments prove that the platform can significantly improve the cDC1 infiltration level of a tumor site, effectively inhibit the progress of prostate cancer (PCa), and provide a new normal form for immune'cold tumor 'treatment.

The invention discloses a platinum (IV)-pirfenidone compound prodrug, a preparation method and microenvironment remodeling anti-tumor application, and belongs to the field of medical technology and drug synthesis. A series of molecules are characterized by being platinum prodrugs modified by pirfenidone derivatives. The synthesis method sequentially comprises the following steps: oxidizing platinum drugs, modifying hydroxyl groups, and introducing pirfenidone derivatives through esterification reaction. A series of molecules are reduced into Pt (II) drugs at the focus to kill cancer cells, and meanwhile, the released pirfenidone derivative can remodel the tumor microenvironment. The platinum prodrug disclosed by the invention not only has excellent cancer cell killing ability, but also is relatively low in drug resistance and excellent in safety, can trigger immunogenic cell death, and is a brand new chemotherapy-immunotherapy method. A series of molecules are convenient to synthesize, the preparation process is simple, and the amphipathicity is excellent, the anti-cancer capability is excellent, the drug resistance and toxicity are low, and the clinical application prospect is good.

The application belongs to the technical field of polymer medicine synthesis, and particularly relates to a polymer for inducing tumor immunogenic cell death, a preparation method and application. The polymer for inducing tumor immunogenic cell death provided by the application has a structure as shown in formula I: in formula I, m is an integer of 110-120; n is an integer of 70-75; p is an integer of 21-79; and R is one of a tertiary amine group, a sulfide group and a tertiary amine sulfide group. The polymer provided by the application has pH responsiveness, can be self-assembled to obtain a polymer nanovesicle, can load drugs, can induce tumor immunogenic cell death (ICD) after application to form an in-situ tumor vaccine, can realize controllable release of a hydrophobic immunoadjuvant, can polarize tumor-associated macrophages (TAMs), and is suitable for tumor immunotherapy application.

The present invention discloses a metal complex tailed with an immunologically active molecule, as well as its preparation method and application. The metal complex tailed with an immunologically active molecule exhibits significant anticancer activity. On the one hand, the metal complex exerts cytotoxicity against rapidly growing cancer cells through its active ingredient with anti-tumor immune efficacy. On the other hand, it can also induce immunogenic cell death, enabling the host immune system to attack tumor cells and induce a tumor-specific immune response. (The metal complex of the present invention has been shown to effectively induce immunogenic cell death in non-small cell lung cancer cells and successfully activate anti-tumor immunity in mice in vaccine experiments.) Therefore, the metal complex of the present invention has outstanding effects in anti-cancer, anti-inflammatory, inducing immunogenic cell death, and achieving anti-tumor immunity, and has broad application prospects.

The invention relates to the technical field of preparation of bone cement, and particularly discloses a preparation method of a photo-thermal response type gold nanorod-bone cement composite material. The method comprises the following steps: synthesizing a gold seed solution; constructing a growth solution; controlling the growth of the nanorod; gradient centrifugal purification; carrying out surface ligand exchange (sulfydryl-PEG); preparing a bone cement composite material; curing and forming; gold nanorods (GNRs) are uniformly embedded into a polymethyl methacrylate (PMMA) bone cement matrix, so that the problems that free nanorods are easy to migrate and poor in bone targeting are solved. The local temperature can be accurately controlled, and damage to surrounding tissues is avoided. According to the invention, immunogen cell death (ICD) can be effectively induced, and anti-tumor immune response can be activated. According to the invention, enough mechanical support can be provided, and the stability after implantation is ensured.

The invention belongs to the technical field of biological medicines, and particularly discloses a pH and ultrasound double-response type oncolytic microorganism as well as a preparation method and application thereof. On the basis of a tumor targeting platform, a mild thermal response gene loop expression GM-CSF and a surface-coated oncolytic microbial system are integrated, chemotherapeutic drugs are released in a tumor core area, and immunogenic cell death is induced. A thermal response loop is accurately activated through low-intensity focused ultrasound, and engineering bacteria are promoted to express GM-CSF and secrete a large amount of mannose modified OMVs. Due to the nanometer size and mannose targeting of the OMVs, the OMVs are efficiently enriched in lymph nodes, the OMVs are reprogrammed into an immune activation state from an immune tolerance state, and the OMVs and ICD cooperate to promote dendritic cell maturation, tumor antigen presentation and activation of tumor killer T cells, so that a remarkable and powerful treatment effect is achieved in various tumor models, and the application prospect is wide. A new strategy is provided for remodeling the lymph node immune microenvironment and enhancing the anti-tumor immune response.

The invention discloses a nano-particle for inducing death of tumor immunogen cells, a preparation method and application. The preparation method of the nano-particles comprises the following steps: taking a BiOCl nanosheet as a carrier, enabling CeO2 to generate and crystallize in situ on the surface of the BiOCl nanosheet, and forming a BiOC / CeO2 heterojunction; the method comprises the following steps: carrying out a dehydration reaction on NH2-PEG2000-NH2 and DL lipoic acid to synthesize NH2-PEG2000-TA; the preparation method comprises the following steps: reacting NH2-PEG (Polyethylene Glycol) 2000-TA with a Pardaxin peptide of which the carboxyl is activated, so as to generate Pardaxin-PEG 2000-TA; the preparation method comprises the following steps of: coupling Pardaxin-PEG2000-TA (Polyethylene Glycol 2000-TA) and a BiOC / CeO2 heterojunction; the PBCP nanoparticles obtained by the preparation method promote the yield of active oxygen in the endoplasmic reticulum of tumor cells to be increased sharply, so that the endoplasmic reticulum stress is effectively excited, tumor immunogenic cells are induced to die, and the anti-tumor effect is achieved. The nanoparticle can be combined with the existing PD-1 / PD-L1 inhibitor for use, and shows a better anti-tumor effect than the single use of the PD-1 / PD-L1 inhibitor.

This invention relates to the fields of brachytherapy and radiosensitization, specifically providing a... 125 I-nano implantable particles, their preparation methods, and applications. 125 The 1-nanometer implanted particles have a core-shell structure, consisting of a particle core and particles adsorbed on the outer surface of the particle core. 125 I and the particles encapsulated in the nucleus and 125 The outer shell of the I-type photosensitizer is a covalent organic framework. This invention... 125 The core of the I-nanometer implanted particle is used to enhance the energy deposition of gamma rays and X-rays, generating more hydroxyl radicals; the shell containing a type I photosensitizer is used to absorb the energy of Auger electrons and internal conversion electrons and excite the photosensitizer to generate singlet oxygen, effectively improving the response to... 125 The efficiency of I decay energy utilization. 125 I-nano implanted particles, combined with internal radiation therapy and radiodynamic therapy, destroy tumor cell DNA and cell membranes, enhance tumor killing and induce immunogenic cell death, thereby activating anti-tumor immunity and inducing remote effects.

The present application relates to a kind of ganoderma lucidum spore as carrier paclitaxel ganoderma lucidum spore oil self-nanoemulsion composite microparticle and its preparation method, belong to medical technology field.The pretreated ganoderma lucidum spore (GLS) is obtained by process screening, and it is used as drug carrier, and the paclitaxel ganoderma lucidum spore oil self-nanoemulsion (PGS) is loaded therein, and paclitaxel composite microparticle (PGS@GLS) is obtained.Release curve in vitro shows that PGS@GLS has the characteristics of slow release after reaching intestinal tract;In vivo pharmacodynamics experiment shows that PGS@GLS can significantly inhibit the development and metastasis of colorectal cancer in mice;Tumor tissue section immunofluorescence analysis shows that PGS@GLS can effectively exert the therapeutic effect on colon cancer.Paclitaxel self-nanoemulsion ganoderma lucidum spore composite microparticle promotes cancer immune cycle by inducing immunogenic cell death and immune regulation, significantly improves the treatment effect of colon cancer, and has good biological safety.

The present invention discloses a nanoparticle for dual-targeted treatment of breast cancer, and its preparation method and application. The nanoparticle for dual-targeted treatment of breast cancer uses polysaccharide-cholesterol hemisuccinate amphiphilic conjugate of Dendrobium officinale as a drug carrier to encapsulate anti-breast cancer chemotherapeutic drugs, and the surface of the drug carrier is modified with folic acid ligands. By using polysaccharide of Dendrobium officinale with immunomodulatory activity as a carrier and combining with folic acid ligands with active targeting function, the present invention realizes dual-targeting of breast cancer and exerts the effect of chemoimmunotherapy. After the prepared nanoparticles are delivered to solid tumors through passive and active targeting, the tumor cells are killed by the released chemotherapeutic drug (doxorubicin), and the immunogenic cell death (ICD) is enhanced by polysaccharide of Dendrobium officinale, promoting the proliferation of natural killer cells (NK cells), stimulating the maturation of dendritic cells, and coordinately regulating various immune cells, while preventing breast cancer lung metastasis.

The present invention relates to the field of biomedicine, and in particular to immunogenic cell death-enhancing nanovesicle hydrogels and their applications. The present invention provides a 3D-printable, multifunctional, synergistic immunogenic cell death-enhancing nanovesicle hydrogel. The hydrogel uses SerMA hydrogel as a matrix and encapsulates cell membrane nanovesicles (N@ILO) loaded with the photothermal agent IR1061, the chemotherapy drug OXA, and the tumor acidic microenvironment regulator Lon. The hydrogel triggers initial tumor immunogenic cell death through the photothermal effect, further enhances the ICD effect through OXA, and modulates the acidic tumor microenvironment through Lon to increase T cell activity, achieving multimodal synergistic anti-tumor therapy after surgery. The hydrogel also possesses excellent photocurable 3D printing capabilities and can be used to construct personalized filling structures that match postoperative defect areas. The hydrogel has broad application prospects and provides a new strategy and theoretical basis for the comprehensive postoperative treatment of choroidal melanoma.

The present application belongs to the technical field of coordination chemistry and biomedical science, and provides a multifunctional iridium (III) complex, a preparation method and application thereof. The multifunctional iridium (III) complex (Mito-Ir) is composed of an iridium (III) complex cation and a coordination anion shown in the following formula. Mito-Ir can efficiently target mitochondria, and also has the abilities of phosphorescence imaging, type I and type II active oxygen generation, and photocatalytic oxidation of nicotinamide adenine dinucleotide. Under light irradiation, Mito-Ir triggers severe mitochondrial dysfunction through the above synergistic effect, and then specifically activates the caspase-3 / GSDME signaling pathway, and significantly induces pyroptosis; this pyroptosis-based cell death mechanism can effectively overcome the apoptosis tolerance of tumor cells, and is accompanied by the release of a large amount of inflammatory factors and damage-associated molecular patterns, stimulates immunogenic cell death, and activates the body's anti-tumor immune response.

The application discloses a kind of SNPs loaded engineered escherichia coli dual-response delivery systems and its preparation method and application, the preparation method includes the engineered escherichia coli of overexpressing Flt3L and TIM-3 scFv is co-incubated with NHS-PEG-CHO, obtain aldehyde functionalized escherichia coli;After activation SNPs is reacted with TFA-hydrazine, obtain hydrazine functionalized SNPs;Aldehyde functionalized escherichia coli and hydrazine functionalized SNPs are reacted;The delivery system of the application can rely on SNPs under laser stimulation Photo-thermal effect, photodynamic effect and generate a large number of ROS, efficiently induce tumor cells to occur immunogenic cell death (ICD), and by overexpressing Flt3L and TIM-3 scFv, activate DC cell and block TIM-3 path, restore DC, T cell function, realize the efficient synergy of photodynamic therapy+photothermal therapy+immunotherapy, and then reach the effective treatment of colorectal cancer.

The invention relates to a nano-composite for promoting thermalization of cold tumors as well as a preparation method and application of the nano-composite. The nano-composite comprises capsaicin and graphene oxide. According to the invention, capsaicin is combined with graphene oxide, so that immunogenic cell death and active oxygen mediated pyroptosis can be promoted, and infiltration and activation of CD4 < + > and CD8 < + > T cells can be remarkably enhanced by the combined immunoreaction, so that potent anti-tumor immunoreaction is induced, and remarkable tumor inhibition is realized.

The invention discloses a cGAS-STING activated micro-needle system and a preparation method and application thereof.The cGAS-STING activated micro-needle system comprises a needle tip and a backing which are connected, the needle tip is loaded with a mesoporous photosensitive nano-composite drug, the mesoporous photosensitive nano-composite drug takes hollow Prussian blue nanoparticles as a carrier, the interior of the mesoporous photosensitive nano-composite drug is loaded with a small molecule drug, and the surface of the mesoporous photosensitive nano-composite drug is coated with a coating. The outer layer is coated with natural CD44 ligand polysaccharide; the small molecular medicine is a mixture of hydroxyurea and carotene. The mesoporous photosensitive nano-composite drug can induce death of immunogenic cells through mild photothermal induction, and release an injury-related molecular mode; meanwhile, a cGAS-STING signal channel is activated, secretion of type I interferon is promoted, and dendritic cells are induced to be activated and mature, so that T cell mediated anti-tumor immune response is enhanced. The invention provides an innovative tumor immunotherapy strategy and has important clinical application value.

This invention discloses a photoactivated nanoparticle-based drug delivery material for immunoadjuvants, its preparation method, and its applications. The drug delivery material is constructed from photosensitizer monomers, crosslinking monomers, and immunoadjuvant prodrug monomers within a confined space of a nanoemulsion via a copper-free click polymerization reaction to form crosslinked polymer nanoparticles. The nanoparticle size is controllable, and the composition is precisely defined. Under physiological conditions, the drug delivery material exhibits good colloidal stability and high drug loading. Upon near-infrared light irradiation, the generated ROS can cleave the thioketone bonds, causing the nanoparticles to depolymerize and achieving photo-triggered, controllable release of the immunoadjuvant. Under photodynamic action, this drug delivery material efficiently generates reactive oxygen species to induce immunogenic cell death in tumor cells. Simultaneously, the released immunoadjuvant activates the TLR7 / 8 pathway, promotes dendritic cell maturation, and enhances the systemic anti-tumor immune response, thereby inhibiting the growth of tumors in situ and at distant sites, and reducing the risk of metastasis and recurrence.

The invention provides a method for preparing calcium-based nano particles by a reversed-phase microemulsion method, an organic calcium salt nano material and application of the organic calcium salt nano material as a nano drug, and belongs to the field of biomedical nano materials. The calcium-based nanoparticles can be formed by reacting calcium dodecyl benzene sulfonate with ammonium salt of corresponding ions or a hydroxyl-containing organic matter aqueous solution; the preparation method of the calcium-based nanoparticles has certain universality, and nano-material drugs including calcium succinate, calcium hypophosphite, calcium gluconate, calcium formate, calcium citrate and the like are successfully synthesized. The synthesis method is simple, mild in reaction condition, environment-friendly, high in repeatability, capable of realizing batch preparation and low in raw material cost. When the calcium salt nano-drug such as calcium succinate enters tumor cells through endocytosis, a large number of ions are brought into the cells, and suddenly excessive ions can cause osmotic pressure and homeostasis imbalance in the cells, activate a pyroptosis pathway of the cells and induce death of immunogenic cells.

The invention belongs to the technical field of biological medicine, and provides an iron-based nanodot capable of guiding MRI and delivering medicine and a preparation method and application of the iron-based nanodot. The iron-based nanodot is used as a chemotherapeutic drug carrier, and is prepared from a cell protective agent amifostine (AT) and key nutritional metal iron ions (Fe < 3 + >) for inducing ferroptosis through a supramolecular self-assembly technology. The iron-based nanodot can efficiently load the chemotherapeutic drug, improves pharmacokinetic behaviors such as short in-vivo circulation period and fast metabolism of the chemotherapeutic drug, accurately delivers the chemotherapeutic drug to a tumor site, and improves the drug effect of the chemotherapeutic drug; in addition, the iron-based nanodots can realize efficient ferroptosis treatment under the guidance of MRI to induce immunogenic cell death and inhibit growth, metastasis and diffusion of tumor cells.

The invention discloses a multi-rotor near-infrared second-region cyanine molecule as well as a preparation method and application thereof, and belongs to the technical field of organic luminescent materials in the field of organic synthesis. The compound provided by the invention has a structural formula as shown in a structural formula (I): # imgabs0 #. The compound provided by the invention can realize near-infrared second-region tumor fluorescence imaging and high-resolution angiography by utilizing the penetration advantage of a near-infrared second region, and can realize near-infrared second-region photothermal immunotherapy by activating tumor immunogenic cell death.

The invention relates to a meso-porous silicon nano-composite for photo-thermal immunotherapy and a preparation method and application thereof. The meso-porous silicon nano-composite comprises meso-porous silicon nano-particles and bismuth nano-particles loaded on the surfaces of the meso-porous silicon nano-particles through electrostatic interaction. According to the invention, a self-enhanced immunotherapy storage cavern is constructed on the basis of loading bismuth nano-particles on mesoporous silicon nano-particles. Under the irradiation of near-infrared laser, the MSN (at) Bi platform shows excellent photothermal conversion efficiency, can locally generate high temperature in vivo and in vitro, and remarkably inhibits the proliferation of tumor cells; mild photothermal therapy of MSN (at) Bi not only directly destroys tumor cells, but also can induce death of immunogenic cells and promote the tumor cells to release damage-related molecular patterns, so that an immune system is activated. According to the invention, a strategy of combining photo-thermal therapy and immunoregulation is adopted, so that the anti-tumor effect is remarkably enhanced, the systematic toxicity is effectively reduced, and an efficient and low-toxicity multifunctional nano medical solution is provided for tumor treatment.

The invention relates to the technical field of biological medicines, and particularly discloses a nanocapsule SMCPM and application thereof in medicines for treating or preventing osteosarcoma and pulmonary metastasis. The SMCPM is composed of a photosensitizer Ce6 carried by mesoporous silicon oxide-manganese oxide particles, an NRF2 inhibitor ML385 and an OS targeting peptide PT-7. Experiments prove that the SMCPM is high in penetrating power and strong in cell killing effect; by down-regulating NRF2 expression and reversing nuclear translocation, a biochemical inhibitory microenvironment can be effectively repaired, the tolerance of cells to active oxygen and epithelial-mesenchymal transition activated and induced by a NOTCH1 signal axis are remarkably reduced, the oxidative stress level of the cells is enhanced, and PIT-induced immunogenic cell death is promoted; the released Mn < 2 + > and double-stranded DNA can regulate and control an immunosuppressive microenvironment by activating an STING pathway in cells, thereby inhibiting the growth of osteosarcoma and preventing lung metastasis.

This invention belongs to the field of biomedical technology, specifically disclosing a nanomicelle FluDox-MIC, its preparation method, and its uses. This nanomicelle is formed by the self-assembly of flumatinib and doxorubicin co-loaded in mPEG-DSPE, exhibiting pH-responsive release properties. Upon entering the tumor microenvironment, it releases the drug. The released flumatinib induces the degradation of the poliovirus receptor (PVR / CD155), relieving its inhibitory signal on NK cells and reactivating innate immune surveillance. Simultaneously, low-dose doxorubicin induces significant immunogenic cell death (ICD), initiating and amplifying tumor-specific T cell-mediated adaptive immunity. Through the synergistic activation of innate and adaptive immunity, the nanomicelle of this invention can effectively inhibit melanoma growth and overcome immunotherapy resistance, showing promising clinical application prospects.

The invention discloses an application of an esterase response cationic polymer in preparation of an anti-tumor immunotherapy drug. The esterase response cationic polymer is an esterase response cationic polymer containing (p-phenolic ester group) benzyl amine or (p-phenolic ester group) benzyl ester. The compound can kill tumor cells, induce immunogen cell death (ICD), promote the tumor cells to release tumor-associated antigens, induce antigen-presenting cells to gather in tumors and present tumor antigens, activate killer T cells, inhibit tumor neovascularization, improve the tumor microenvironment, inhibit tumor cell apoptosis, inhibit tumor cell apoptosis, inhibit tumor cell apoptosis and inhibit tumor cell apoptosis after intratumor injection. Therefore, the organism obtains the anti-tumor immunity capability.

The invention relates to the technical field of medical treatment, in particular to a preparation method of injectable BiOCl (at) CuOF127 (at) rGO thermosensitive hydrogel for superficial tumor treatment, which comprises the following steps: pretreating raw materials, preparing BiOCl nanosheets by a solvothermal method, washing and drying for later use; according to the manufacturing method of the injectable BiOCl (at) CuOF127 (at) rGO thermosensitive hydrogel for superficial tumor treatment, the injectable BiOCl (at) CuOF127 (at) rGO thermosensitive hydrogel is embedded into injectable, conductive and thermosensitive hydrogel composed of PluronicF127 (F127) and reduced graphene oxide (rGO), the injectable BiOCl (at) CuOF127 (at) rGO thermosensitive hydrogel is used for combined treatment of breast cancer, remarkable ROS outbreak and effective breast cancer cell ablation are induced through in-vitro and synergistic therapy, toxicity to normal cells is minimum, and the injectable BiOCl (at) CuOF127 (at) rGO thermosensitive hydrogel can be applied to in-vivo and in-vivo treatment of breast cancer. In-situ breast tumors treated with US + NIR show obvious fading, proliferation and angiogenesis reduction, apoptosis and immunogen cell death pathway activation and good biocompatibility, an accurate and minimally invasive strategy is established, piezoelectric catalysis and photo-thermal conversion are combined, and the in-situ breast tumors are used for effective breast cancer treatment.