941 results about "Cytotoxicity" patented technology

The invention belongs to the technical field of cosmetics, and discloses anti-aging recombinant collagen as well as a preparation method and application thereof. The amino acid sequence of the anti-aging recombinant collagen is as shown in SEQ ID NO.1, and the nucleotide sequence of the anti-aging recombinant collagen is as shown in SEQ ID NO.2. The recombinant collagen CaI1 is constructed and expressed by recombining the effective sequences of the I-type collagen, the III-type collagen and the XVII-type collagen, and the recombinant collagen CaI1 is free of cytotoxicity, free of hidden danger of endotoxin and good in safety. The skin care product added with the recombinant collagen CaI1 can promote skin collagen absorption, inhibit skin aging and improve skin elasticity, and has a good application prospect.

The invention relates to the technical field of biology, and particularly discloses a recombinant collagen III and application thereof in preparation of gel. The recombinant collagen III is designed by optimizing functional area sequences of human I-type and III-type collagen, the amino acid sequence is shown as SEQ ID No.2, and the recombinant collagen III has the characteristics of high stability, good hydrophilicity and low immunogenicity. The preparation method comprises the steps of expression vector construction, escherichia coli induced expression, affinity chromatography purification and renaturation. The recombinant collagen III can be prepared into a gel dressing and comprises sodium alginate, methylparaben and other components. Experiments show that the gel can effectively promote cell proliferation and has no cytotoxicity; in a mouse skin injury model, the collagen can relieve inflammation, accelerate wound healing and inhibit scar formation, and the effect of the collagen is superior to that of natural human III-type collagen. The invention provides a safe and efficient novel material for wound repair, and is suitable for medical dressings and tissue engineering.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide and application thereof. The amino acid sequence of the antibacterial peptide is shown as SEQ ID NO: 1, the number of amino acids is small, the synthesis cost is low, the antibacterial peptide has high antibacterial activity on gram-negative bacteria and gram-positive bacteria, the minimum inhibitory concentration of the antibacterial peptide on acinetobacter baumannii, escherichia coli, pseudomonas aeruginosa and staphylococcus aureus is 4.68-37.5 mu g / mL, and the antibacterial peptide has the advantages of broad-spectrum antibacterial property, good stability and good application prospect. No obvious cytotoxicity and hemolysis effect exist; moreover, end-to-end amido bond cyclization is carried out, so that the antibacterial activity is further enhanced, the antibacterial effect on acinetobacter baumannii, escherichia coli, pseudomonas aeruginosa and staphylococcus aureus is enhanced by 2-4 times, and the minimum inhibitory concentration is reduced to 2.34-9.38 mu g / mL; after the end-to-end amido bonds are cyclized, the plasma stability and safety of the antibacterial peptide are further improved, and the cytotoxicity and hemolysis are reduced.

To provide an antitumor drug having an excellent therapeutic effect, which is excellent in terms of antitumor effect and safety. Provided is an anti-cadherin 17 antibody comprising a heavy chain variable region and a light chain variable region, a drug conjugate thereof, and a composition containing the anti-cadherin 17 antibody or the drug conjugate thereof, and an application thereof as a drug.

The invention discloses a preparation method of a three-dimensional porous white phosphorus calcium stone ceramic bracket with piezoelectric biological activity, and belongs to the field of brackets. The preparation method of the scaffold comprises the following steps: uniformly dipping a polymer foam template with a certain pore size and porosity in slurry composed of beta-tricalcium phosphate and a binder, carrying out high-temperature sintering to remove organic matters, carrying out a hydrothermal reaction on the inorganic scaffold in an aqueous solution containing magnesium ions, converting beta-tricalcium phosphate into white calcomanite, and carrying out drying to obtain the scaffold. And then carrying out high-temperature annealing treatment to obtain the white calcomanite porous ceramic bracket with piezoelectric biological activity. The prepared ceramic scaffold is controllable in composition and degradation rate, excellent in mechanical property, electroactive, free of cytotoxicity and remarkable in osteogenesis induction effect, and the porosity and aperture of the scaffold are suitable for cells to enter and grow. The preparation method of the white phosphorite three-dimensional piezoelectric ceramic bracket is simple and easy to implement, controllable in process and easy to amplify.

The invention provides a thiazolidinone compound and application thereof in preparation of drugs for resisting staphylococcus aureus infection. The molecular structural formula of the thiazolidinone compound is shown as a formula (1), a formula (2), a formula (3) or a formula (4). The technical scheme of the invention discloses several thiazolidinone compounds, which not only have an excellent bactericidal effect on staphylococcus aureus and can significantly inhibit the formation of a biofilm of staphylococcus aureus, but also have low cytotoxicity and hemolytic activity. According to the technical scheme, a certain foundation is laid for research and development of novel staphylococcus aureus biofilm infection resisting drugs, and the potential of thiazolidone derivatives for developing novel antibacterial agents for staphylococcus aureus related infection can be deeply known possibly.

The invention discloses application of scylla serrata antibacterial peptide Scycin in preparation of an anti-inflammatory composition and a preparation method of the scylla serrata antibacterial peptide Scycin. The nucleotide sequence of the scylla serrata antibacterial peptide Scycin is shown as SEQ ID NO.01. The invention further discloses a preparation method of the scylla serrata antibacterial peptide Scycin. In an LPS (lipopolysaccharide)-induced RAW 264.7 macrophage inflammation model, the recombinant expression plasmid vector containing the scylla antibacterial peptide Scin remarkably inhibits an inflammation cascade reaction by down-regulating expression of proinflammatory factors such as TNF-alpha (tumor necrosis factor-alpha) and IL-6 (interleukin-6), the anti-inflammatory effect of the recombinant expression plasmid vector is equivalent to that of a mesalazine positive control group, and obvious cytotoxicity is not observed.

The present invention provides an antibody or fragment thereof having binding specificity for human solute carrier 34A2 (SLC34A2) protein. These antibodies are capable of binding to SLC34A2 with high affinity, and can mediate antibody dependent cytotoxicity (ADCC) and effectively induce endocytosis. Methods and uses for treating cancer are also provided.

The invention provides an ervatamia divaricata extract as well as a preparation method and application of the ervatamia divaricata extract in resisting brain metastatic tumor. Pharmacological activity shows that the extract of ervatamia divaricata disclosed by the invention has remarkable cytotoxicity inhibition activity, and the inhibition effect of the extract is time-dependent and dose-dependent. The extract can inhibit the growth of breast cancer 4T1 brain metastases, and has no obvious adverse reaction on mice.

The invention belongs to the technical field of specific fluorescent probe detection, and particularly relates to a near-infrared fluorescent probe for detecting hydrazine hydrate as well as a preparation method and application of the near-infrared fluorescent probe. The structural formula of the near-infrared fluorescent probe is # imgabs0. The invention provides a preparation method for synthesizing the probe by taking xanthene dye and acetyl chloride as raw materials. By researching the fluorescence spectrum of the fluorescent probe, the selectivity of the probe on N2H4 is evaluated, the fluorescence intensity of the probe is determined to be obviously enhanced only in the presence of N2H4, and the fluorescence intensity is positively correlated with the concentration of N2H4; meanwhile, the probe has the characteristics of quick response and near-infrared emission; the fluorescent probe disclosed by the invention has relatively small cytotoxicity and can be applied to detection of HepG2 cell exogenous hydrazine hydrate.

The invention discloses high-purity recombinant collagen as well as a preparation method and application thereof, the recombinant collagen is composed of a recombinant collagen monomer, and the recombinant collagen monomer has an amino acid sequence as shown in any one of SEQ ID NO: 1-22. According to the present invention, the easy-to-break site and the hydrophobicity of the collagen are optimized, such that the obtained recombinant collagen monomer and the prepared recombinant collagen have characteristics of high cell activity (adhesion, migration and proliferation), high triple helix structure stability, high purity, no cytotoxicity, large-scale production, and production cost reduction.

The invention belongs to the field of biological medicine, and particularly relates to an antibacterial peptide for inhibiting staphylococcus aureus and pseudomonas aeruginosa and application of the antibacterial peptide. The antibacterial peptide CAMP648NC is a novel cationic antibacterial peptide, the minimum inhibitory concentrations of the antibacterial peptide CAMP648NC to pathogenic bacteria staphylococcus aureus and pseudomonas aeruginosa are 16 [mu] g / mL and 32 [mu] g / mL respectively, the antibacterial peptide CAMP648NC has extremely low cytotoxicity, and after human skin keratinocytes HaCat and bronchial epithelial cells BEAS-2B are treated by the antibacterial peptide CAMP648NC with the concentration of 128 [mu] g / mL for 24 hours, the survival rates of 110.4% and 87.3% are still kept. Due to the characteristics, the compound shows extremely high medical drug antibacterial value, and alternative medicinal resources are provided for clinically resisting the two human pathogenic bacteria in the future.

Described herein are polypeptides and related antibodies comprising a variant Fc domain. The variant Fc domain provide for stabilized Fc: Fc interactions when the poly-peptide(s), antibody or antibodies are bound to its target, antigen or antigens on the surface of a cell, thus providing for improved complement-dependent cytotoxicity (CDC).

The invention belongs to the technical field of polypeptides and biological medicines, and particularly relates to a WK7 series antibacterial peptide and application thereof in the aspects of resisting bacteria and preventing and treating diabetic foot, pneumonia and septicemia. According to the invention, a linear peptide WK7-line with an amino acid sequence of WKRWKRW is used as a minimum active unit, and modification is carried out to form a plurality of linear modified peptides; meanwhile, a cyclic peptide WK7-cyl is formed on the basis of the linear peptide WK7-line through cyclization, and the cyclic peptide WK7-cyl is further modified to form an annular modified peptide. The linear peptide WK7-line, the linear modified peptide, the cyclic peptide WK7-cyl and the cyclic modified peptide jointly form the WK7-series antibacterial peptide, and the WK7-series antibacterial peptide is artificially synthesized polypeptide, is small in molecular weight, easy to synthesize, free of cytotoxicity, almost free of hemolysis risk, high in sterilization speed and broad-spectrum antibacterial property and can be used for preparing the antibacterial peptide. The traditional Chinese medicine composition has a certain effect on prevention and treatment of diabetic foot ulcer, pneumonia and septicemia.

The invention discloses a mussel byssus polypeptide with antibacterial performance and application thereof.The mussel byssus polypeptide has an obvious inhibiting effect on gram-positive bacteria and gram-negative bacteria, can inhibit formation of biological membranes of the gram-positive bacteria and the gram-negative bacteria, shows different antibacterial activities on multiple drug-resistant bacteria, and has the advantages that the mussel byssus polypeptide can be used for preparing a drug-resistant agent; and the hydrogel has low cytotoxicity and good biocompatibility, and shows good adhesion capability. The polypeptide is short in peptide chain and small in molecular weight, is a micromolecular polypeptide with great value, shows wide application potential in multiple fields, and has important value in the fields of medicine preparation, medical equipment and daily chemical products.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide Mh-GC21 as well as a precursor and application thereof. The amino acid sequence of the antibacterial peptide Mh-GC21 provided by the invention is as shown in SEQ ID NO.1, and two cysteines are oxidized to form a pair of intermolecular disulfide bonds; the C tail end of the antibacterial peptide Mh-GC21 is subjected to amidation modification. The antibacterial peptide Mh-GC21 provided by the invention is from microhyla microcarpa, has broad-spectrum antibacterial activity, has relatively good antibacterial and bactericidal effects on standard strains of acinetobacter baumannii, escherichia coli and staphylococcus aureus and clinically sourced drug-resistant strains, and can be used for targeted destruction of cell membranes of bacteria, removal of biological membranes and inhibition of formation of the biological membranes. Moreover, the antibacterial peptide Mh-GC21 has the advantages of good stability, no hemolytic activity and cytotoxicity, and difficulty in inducing strains to generate drug resistance.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an LL-37 derived antibacterial peptide and application thereof. According to the invention, 17-29aa of the LL-37 antibacterial peptide is used as a core fragment and is connected with an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2 through an amido bond, the antibacterial activity of the obtained antibacterial peptide is obviously higher than that of the LL-37 antibacterial peptide, and the antibacterial peptide shows an obvious bactericidal effect on clinical drug-resistant bacteria such as acinetobacter baumannii, pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, klebsiella pneumoniae and the like; meanwhile, the cytotoxicity and hemolytic activity are weak, drug resistance is not prone to being generated, bacteria can be rapidly killed in vivo through multiple mechanisms, no obvious cytotoxicity exists, the bacterium load in tissue is remarkably reduced, and the lifetime of a bacterium-infected mouse is prolonged.

The invention relates to a Cu2-xSe-Pt nano material, a microneedle patch, a preparation method and application. The Cu < 2-x > Se (at) Pt nano material is composed of a Cu < 2-x > Se nanosphere and platinum modified on the surface of the Cu < 2-x > Se nanosphere in situ. The invention provides a preparation method of a Cu2-xSe-Pt nano-material, which comprises the following steps: adding PSS, SeO2 and AA into water, heating and stirring, adding copper salt and AA, reacting, and adding water and a hexachloroplatinic acid solution to obtain the Cu2-xSe-Pt nano-material. The invention provides an application of a Cu < 2-x > Se (at) Pt nano material. The invention also provides a microneedle patch as well as a preparation method and application thereof. The invention provides a novel material for resisting multi-drug-resistant bacteria, which solves the problems that the existing single antibacterial strategy is limited and the expected curative effect cannot be achieved, also solves the problem of cytotoxicity of the existing nano enzyme, realizes deep drug delivery, ensures efficient local drug release and avoids systemic toxicity.

The invention relates to the technical field of biology. The invention provides a grass carp brain cell line sensitive to grass carp reovirus type II, which is preserved in China Center for Type Culture Collection on October 19, 2024, and the preservation number is CCTCC NO: C2024101. The constructed grass carp brain cell line shows remarkable stability, and stable passage is successfully achieved for 60 times. And when GCRV-II is inoculated, an atypical cytopathic effect can be presented. In addition, the grass carp brain cell line has the capability of efficiently replicating GCRV-II, and even if blind passage is carried out for three generations, generated cytotoxicity can still cause morbidity and death of the grass carp. Therefore, a stable foundation is built for deeply exploring the pathogenic mechanism of the GCRV-II, developing vaccine development, screening antiviral drugs and the like, research in related fields is promoted to further progress, and a key cell model and a research basis are provided for development of related research and prevention and control strategies of the grass carp reovirus.

The invention belongs to the technical field of carbon material luminescent materials, and particularly relates to a preparation method of a multicolor luminescent carbon dot and bioimaging application of the multicolor luminescent carbon dot. The invention provides a simple and controllable multicolor luminescent carbon dot and a preparation method thereof. By adjusting the reaction time in the heat treatment process of o-phenylenediamine and thiourea solvents, various luminescent carbon dots, including green light, yellow light and red light carbon dots, are successfully separated. The carbon dot provided by the invention can realize controllable adjustment of fluorescence performance by regulating and controlling the conjugation degree of the carbon core and the content of graphite nitrogen. According to the method, the fluorescence mechanism in the carbon dot forming process is understood more deeply, and guidance is provided for designing the structure and optical performance of the carbon dots and achieving controllable synthesis of the high-performance carbon dots. The multicolor luminescent carbon dot provided by the invention has excellent optical performance, stable morphological structure and low cytotoxicity, and has a good application prospect in biological imaging.

The invention discloses application of domoic acid in preparation of a largemouth bass frog virus inhibitor. The domoic acid disclosed by the invention is relatively low in cytotoxicity, and has an obvious inhibition effect on micropterus salmoides frog virus compared with other microalgal toxins. Further exploration shows that after the micropterus salmoides frog virus is in direct contact with the domoic acid for 12 hours, the micropterus salmoides frog virus is irregular in shape and is completely bright white, so that the domoic acid has a direct destructive effect on the micropterus salmoides frog virus. And the domoic acid can inhibit the process of releasing the micropterus salmoides frog virus to the outside of the cells.

The invention discloses a pyridone compound as well as a synthesis method and application thereof. The invention discloses a pyridone derivative which has a structure as shown in a general formula I, a general formula II or a general formula III, the invention also discloses a preparation method and the like of the derivative. Researches show that the drug resistance index (RI = IC50 [I38T] / IC50 [WT]) of the hydroxypyridone derivative disclosed by the invention in RNA (Ribonucleic Acid) polymerase of wild type and PA / I38T mutant type influenza viruses is smaller than that of baloxvir, which indicates that the derivative disclosed by the invention is less influenced by the influenza A PA / I38T mutant type and is superior to the baloxvir. Moreover, the cytotoxicity of the derivative is lower than that of baloxvir, so that the derivative has relatively high selectivity and can be applied to preparation of anti-influenza drugs for inhibiting baloxvir drug-resistant virus strains. # imgabs0 #

Illumination devices for impinging light on tissue, for example within a body cavity of a patient, to induce various biological effects are disclosed. Biological effects may include at least one of inactivating and / or inhibiting growth of one or more pathogens, upregulating a local immune response, increasing endogenous stores of nitric oxide, releasing nitric oxide from endogenous stores, and inducing an anti-inflammatory effect. Biological effects may include upregulating and downregulating inflammatory immune response molecules within a target tissue. Wavelengths of light are selected based on intended biological effects for one or more of targeted tissue types and targeted pathogens. Light treatments may provide multiple pathogenic biological effects, either with light of a single wavelength or with light having multiple wavelengths. Devices for light treatments are disclosed that provide light doses for inducing biological effects on various targeted pathogens and tissues with increased efficacy and reduced cytotoxicity.

The present invention relates to a method for preparing an acellular dermal matrix derived from a human body, in which the acellular dermal matrix is washed for the second time with an alcohol-based degreasing solution, thereby not only having excellent safety against cytotoxicity, but also being able to effectively remove residual fat.

The invention belongs to the technical field of biological medicine, and discloses an anti-BCMA antibody or an antigen binding fragment thereof as well as a preparation method and application thereof. The amino acid sequences of the heavy chain variable regions CDR1-3 of the anti-BCMA antibody or the antigen binding fragment thereof provided by the invention are respectively shown as SEQ ID NO: 2-4, and the amino acid sequences of the light chain variable regions CDR1-3 are respectively shown as SEQ ID NO: 6-8. The anti-BCMA antibody or the antigen binding fragment thereof has excellent affinity for the BCMA protein, especially an extracellular fragment, can specifically recognize and bind the BCMA protein well, and can be used as a detection antibody of the BCMA protein to realize non-diagnostic purpose detection of the BCMA; meanwhile, the anti-BCMA antibody or the antigen binding fragment thereof can also effectively kill tumor cells overexpressing BCMA, such as pfeffer cells, through complement-dependent cytotoxicity, the cell killing rate is up to 99%, and the anti-BCMA antibody or the antigen binding fragment thereof can be used as an inhibitor of abnormal bone marrow cells overexpressing BCMA protein, is a very potential active ingredient for resisting malignant tumors of a blood system, and has broad application prospects. Good application prospects are realized.

The invention discloses a broad-spectrum antibacterial peptide as well as a screening method and application thereof, and belongs to the technical field of antibacterial peptides, a bioinformatics method is mainly adopted for searching a new antibacterial peptide, then amino acid sequence optimization is carried out, and finally the antibacterial peptide with a better antibacterial effect is obtained. Sterilization activity evaluation is carried out by using various known strains with drug resistance, and the result shows that the antibacterial peptide provided by the invention has broad-spectrum antibacterial activity on drug-resistant bacteria, more comprehensive antibacterial effect and wider indications; the compound has the advantages of no hemolysis, low cytotoxicity, efficient in-vivo antibacterial activity and high stability, and has application safety; the compound has a wide application prospect of being developed into a human body antibacterial drug.

The invention relates to the technical field of antibacterial peptides, in particular to an antibacterial peptide RF-18 and application thereof in preparation of antibacterial products. The amino acid sequence of the antibacterial peptide RF-18 provided by the invention is as shown in SEQ ID NO. 1. The antibacterial peptide RF-18 provided by the invention is an antibacterial peptide capable of being artificially synthesized and only contains 18 amino acids, and all the amino acids are L-type amino acids, so that the production cost is greatly reduced; besides, the antibacterial peptide RF-18 provided by the invention is a novel antibacterial peptide with an imperfect amphiphilic structure, not only can exert efficient and broad-spectrum antibacterial activity, but also has extremely high safety, has no cytotoxicity or hemolysis effect in a concentration range of 800 mu g / mL, and can be used for preparing antibacterial infection drugs.

The invention relates to the technical field of biology, and particularly discloses a polypeptide and application thereof in enhancing immunity and / or resisting inflammation. The invention provides a polypeptide. The amino acid sequence of the polypeptide is shown as SEQ ID NO. 1. The compound has the dual functions of regulating immunity and resisting inflammation, on one hand, the immune function of cells under the normal condition can be enhanced, and on the other hand, cell inflammation can be relieved by reducing the phosphorylation level of key protein in an LTR4-NF-kappa B signal channel in the inflammatory state. The polypeptide is low in cytotoxicity, high in safety, simple in preparation method and low in cost, can be used as an ideal immune and anti-inflammatory regulator, and has good application potential and value.

The invention belongs to the technical field of biology, and particularly relates to an antibacterial peptide for inhibiting propionibacterium acnes and application of the antibacterial peptide. The antibacterial peptide for inhibiting propionibacterium acnes is derived from marine organism hylophila, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1. The amino acid sequence is novel, has remarkable antibacterial activity on propionibacterium acnes, has the advantages of low hemolysis toxicity, low cytotoxicity and high safety, and can be used for preparing the antibacterial peptide for inhibiting propionibacterium acnes. Due to the characteristics, the compound has huge application potential in the aspect of treating acnes caused by propionibacterium acnes or removing acnes.

The invention belongs to the technical field of polypeptide drugs in biochemistry, and discloses three antibacterial peptide analogues as well as a preparation method and application thereof, the sequence of an antibacterial peptide P-1 is CH3CO-GGGENIKKILSKIKLLK-NH2, the sequence of an antibacterial peptide P-2 is CH3CH2CH2CO-GGGENIKKILSKIKLLK-NH2, the sequence of an antibacterial peptide P-3 is CH3 (CH2) 6CO-GGGENIKKILSKIKLLK-NH2, the carboxyl ends of polypeptides are subjected to amidation, and the polypeptides are all positively charged when the pH is equal to 7. The artificially synthesized antibacterial peptide disclosed by the invention shows a remarkable sterilization effect on multidrug resistant staphylococcus epidermidis. P-1, P-2 and P-3 have small molecular weight, are convenient to synthesize, have broad-spectrum bactericidal activity, are self-assembled into nanoparticles, have low cytotoxicity, resist trypsin degradation and the like, and have wide application prospects.