128 results about "Carp" patented technology

The invention discloses application of an overexpressed carp lpla gene in regulation and control of expression of cyprinid fish tissue-related genes, and relates to the field of gene engineering, in particular to application of the overexpressed carp lpla gene in regulation and control of cyprinid fish tissue-related genes. The overexpressed carp lpla gene is applied to regulation and control of contents of lipid indexes of total cholesterol TC and triglyceride TG of different tissues of cyprinid fishes. The invention further discloses application of the overexpressed carp lpla gene in regulation and control of fat marker genes lpla, ppar gamma, Fabp3, fast and acaca of livers and muscle tissues of cyprinid fishes. The invention further discloses application of the overexpressed carp lpla gene in regulating and controlling the relative expression quantity of carp liver cell fat metabolism related genes lpla, ppar gamma, Fabp3, fast, acaca, hsl and pnpla2. The overexpressed carp lpla gene can regulate and control lipid deposition in different tissues in zebra fish, and a research model is provided for lpla to regulate and control fat deposition and differentiation. According to the overexpressed carp lpla gene disclosed by the invention, lpla high-expression carp hepatocytes are obtained through a liposome transfection technology, so that fat key gene expression in the carp hepatocytes is changed.

The invention discloses a molecular marker related to high-sugar feed utilization characters of siniperca chuatsi and application of the molecular marker. The molecular marker is located on a No.7 chromosome of siniperca chuatsi, and the molecular marker contains one SNP (Single Nucleotide Polymorphism) site slc25a11-1; the SNP site slc25a11-1 is located at the 1439370th basic group of the No.6 chromosome of siniperca chuatsi, and the polymorphic site is T / C. By implementing the molecular marker disclosed by the invention, the high-sugar feed utilization condition of siniperca chuatsi species can be remarkably improved, so that the feed cost is reduced, and the culture benefit is increased.

The invention discloses a molecular marker related to excellent feed utilization characters of siniperca chuatsi and application. The molecular marker is located on a No.15 chromosome of siniperca chuatsi, and the molecular marker contains one SNP (Single Nucleotide Polymorphism) site kmt2e-1; the SNP site kmt2e-1 is located at the 10368245th basic group of the No.15 chromosome of siniperca chuatsi, and the polymorphic site is C / T. According to the invention, SNP typing based on feed utilization characters is realized in siniperca chuatsi for the first time, and the accuracy and efficiency of genetic breeding are improved.

The invention belongs to the technical field of fish ecology, and particularly relates to a nude carp otolith embedding method based on a longitudinal section and a preparation method and application of an otolith grinding sheet. The epoxy resin and the curing agent are mixed according to a certain mass ratio and then placed on the vegetable parchment to embed the longitudinal section of the naked carp otolith, the vegetable parchment is resistant to high temperature and high in strength and is not prone to deformation caused by high temperature, and the cured embedding piece is easily separated from the vegetable parchment; the embedding agent is low in shrinkage rate after being cured, the otolith is not prone to fracture, the hardness requirement of subsequent grinding can be met, adhesion is high, degumming is avoided in the subsequent grinding process, and fragmentation of the naked carp otolith is effectively avoided. According to the method, the embedding piece polished for the first time is immediately immersed in water, oxidation of the polished surface of the otolith can be prevented, subsequent polishing is continued, and the otolith grinding piece with clear wheel lines is obtained. The method is simple in operation process, does not need to use a high-cost and low-efficiency embedding mold, reduces the preparation cost, and improves the preparation efficiency.

The invention discloses an environment-friendly device with an automatic feeding function for carp culture in the field of aquaculture. The environment-friendly device comprises a material carrying disc and a floating disc which are sequentially connected from top to bottom, the interior of the material carrying disc and the interior of the floating disc are hollow, and a vertical threaded rod penetrates through the center of the material carrying disc and the center of the floating disc; a plurality of first supporting rods are connected between the material carrying disc and the threaded rod, the first supporting rods divide the material carrying disc into a plurality of bearing blocks, each bearing block comprises a free end and a connecting end, the connecting ends are hinged to the interiors of the bearing blocks, the free ends surround the first supporting rods, and feed is placed on the surfaces of the bearing blocks; a second supporting rod is connected between the floating disc and the threaded rod, the second supporting rod protrudes towards the material carrying disc, the second supporting rod divides the material carrying disc into a plurality of bearing nets, the second supporting rod and the bearing nets are located in the moving stroke of the bearing block, and according to the technical scheme, the bearing block is jacked up, so that feed enters the pond for automatic feeding.

The invention discloses a cross breeding method for anti-ichthyophthirius mongholicus schizothorax prenanti. The method comprises the following steps: 1) selecting parents; (2) carrying out intensive cultivation on the parents; 3) synchronous artificial spawning induction; 4) artificial insemination; 5) hatching management; (6) breeding the schizothorax prenanti (female) and percocypris pingi (sex) hybrid fry; and (7) measuring the ichthyophthirius multifiliis resistance and high-temperature tolerance of the schizothorax prenanti (female) * percocypris pingi (sex) hybrid fry. The ichthyophthirius ichthyophthirius infection rate of the bred schizothorax prenanti (female) and percocypris pingi (sex) hybrid offspring is reduced by 31.95% compared with that of the schizothorax prenanti (female) and percocypris pingi (sex) selfing offspring; the death rate is only 0.33% and is reduced by 73% compared with that of the selfing filial generation of the schizothorax prenanti; the high-temperature tolerance range of the schizothorax prenanti selfing offspring is improved by 4-5 DEG C compared with that of the schizothorax Therefore, the method disclosed by the invention can be used for successfully culturing a hybrid variety which is high in ichthyophthirius multifiliis resistance, relatively high in economic value and relatively high in high-temperature tolerance.

The present invention discloses a skin mucus protein molecular marker for accurately quantifying the alkalinity stress level of fish and a detection method thereof, belonging to the technical field of fish alkali tolerance. The present invention aims to solve the problem that there is currently a lack of protein molecular markers for the alkalinity stress level of fish, which in turn affects the efficiency of fish alkali tolerance breeding and selection. The present invention provides a marker for identifying carp fish with alkali tolerance, and the amino acid sequence of the marker is shown in SEQ ID NO.1. It will provide important support for the genetic breeding and selection of alkali-tolerant fish such as grass carp, crucian carp, and Leuciscus waleckii, and further promote the fishery development and utilization of medium and high-saline-alkali waters.

The present disclosure is concerned with compounds and compositions for use in the prevention and treatment of cancer such as, for example, a primary or secondary tumor within a subject's brain, breast, kidney, pancreas, lung, colon, prostate, lymphatic system, liver, ovary, or cervix. Additional examples of cancers for which the disclosed compounds and compositions can be useful include, but are not limited to, sarcomas, carcinomas, hematological cancers, solid tumors, breast cancer, cervical cancer, gastrointestinal cancer, colorectal cancer, brain cancer, skin cancer, prostate cancer, ovarian cancer, thyroid cancer, testicular cancer, pancreatic cancer, liver cancer, endometrial cancer, melanomas, gliomas, leukemia, lymphoma, chronic myeloproliferative disorders, myelodysplastic syndrome, myeloproliferative neoplasm, non-small cell lung carcinomas, and plasma cell neoplasms (myelomas). This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

This invention relates to the field of aquatic compound feed technology, specifically to a special feed for mullet, silver carp, and golden carp cultured in saline-alkali land in Northwest China. The feed is characterized by being composed of the following raw materials by weight percentage: 10%–14% alfalfa meal, 16%–22% soybean meal, 16%–22% corn meal, 5%–7% molasses, 1%–3% yeast powder, 2%–5% lactic acid bacteria preparation, 2%–5% premixed feed for aquatic life in saline-alkali land in Northwest China, 1%–4% fish oil, and 1%–5% vegetable oil. This invention solves the problems of low survival rate, high feed conversion ratio, easy antagonistic inactivation of probiotics, fish oil oxidation, poor water resistance of pellets, and insufficient regional adaptability of traditional feeds. Compared with commercially available ordinary marine fish feeds, the survival rate of juvenile fish is increased by 15% to 30%, the feed conversion ratio is reduced by 10% to 20%, the Vibrio inhibition rate is ≥80%, the power consumption per ton of feed is reduced by 25% to 35%, and the raw material cost is reduced by 30% to 45%. It is suitable for large-scale aquaculture in saline-alkali land in Northwest China and has important value for improving the quality and efficiency of fisheries, ecological restoration, and rural revitalization.

The present invention belongs to the field of animal molecular biology DNA marker technology and application technology, and specifically discloses SNP molecular markers related to the growth traits of South China carp. The present invention screens out SNP markers and typing information related to the fast growth traits of South China carp by performing whole-genome resequencing and genome-wide association analysis on fast-growing individuals and slow-growing individuals in the same South China carp population. Specific primers are designed based on the sequence of the molecular marker, which can quickly and accurately detect sites related to growth. By detecting the SNP marker, fast-growing South China carp can be effectively selected under the same breeding conditions. It can be effectively used in molecular marker-assisted breeding of South China carp, providing technical support for breeding.

Comp / Carp fishing missile.

The invention discloses a high-yield carp and crayfish polyculture system in a cold region pond, relates to the high-yield carp and crayfish polyculture system in the cold region pond, and belongs to the field of biological behavior ecology and the technical field of aquaculture. The invention aims to break through the ecological constraint of the special habitat of the cold region on the crayfish and solve the problem of the economic benefit bottleneck of the main culture pond for the carps in the cold region. The system comprises a carp feeding area, an annular submerged plant zone and a main crayfish breeding area, and the area ratio of the carp feeding area to the annular submerged plant zone to the main crayfish breeding area is (1-1.5): (0.5-1.0): (10.5-9.5). According to the method, the economic benefit bottleneck of the carp main culture pond is solved, the survival trapping of the crayfish is broken through, and the ecological constraint brought by the special habitat of the cold region is tamed.

The present application relates to a kind of carp herpesvirus II type ORF150 gene deletion strain and its preparation method and application, belong to aquatic animal disease prevention and control technical field.The present application carp herpesvirus II type ORF150 gene deletion strain preservation number is CCTCC NO:V202603, is preserved in China typical culture preservation center, and is named carp herpesvirus 2 type CyHV-2-SS24-Δ150-GFP Cyvirus cyprinidallo2 The virulence of the present application carp herpesvirus II type ORF150 gene deletion strain is significantly weakened, but still retains good immunogenicity, can induce the immune fish body to produce immune protection to carp herpesvirus II type.ORF Therefore, the present application carp herpesvirus II type ORF150 gene deletion strain can be used as live attenuated vaccine to carp herpesvirus II type, prevent koi and its variety in aquaculture from being infected by carp herpesvirus II type and lead to hematopoietic organ necrosis disease.

The present application relates to the fields of biotechnology and agricultural fishery, and discloses application of star anise water extract in preparation of an inhibitor of CyHV-2, and the technical scheme finds that the star anise water extract can effectively inhibit the infection and replication of CyHV-2 virus in GiCF cells. The present application firstly applies the star anise water extract to prevent and treat the infection of CyHV-2 (CyHV-2), thereby providing a new treatment scheme for CyHV-2 virus. The star anise water extract can effectively reduce the copy number of CyHV-2 virus, and the star anise water extract can be used to prepare a medicine for preventing and treating the infection of CyHV-2, and a medicine for treating viral hematopoietic organ necrosis disease of fish.

1. The name of the designed product: receiver (H19 European carp fishing alarm). 2. The use of the designed product: for receiving the signal of alarm transmission. 3. The design points of the designed product: in shape. 4. The picture or photo that best shows the design points: perspective view 1.

1. Name of the product in this design: Fishing Rod (Masterful Carp). 2. Purpose of this design: Fishing rod for fishing. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: a 3D model.

1. Name of the design product: wine bottle (year of the carp). 2. Use of the design product: applied to wine bottle. 3. Design points of the design product: combination of shape and pattern. 4. Picture or photo best indicating the design points: perspective view.

The invention belongs to the technical field of aquaculture, and relates to a new application of capsaicin, in particular to an application of capsaicin in preparation of drugs and / or feed additives for preventing and treating fish virus infection. The core of the invention lies in that the capsaicin has an obvious protection effect on infection of spring viraemia of carp virus (SVCV), grass carp reovirus (GCRV) and herpesvirus type 2 (CyHV-2), and can obviously inhibit transcription of SVCV, GCRV and CyHV-2 virus genes and expression of virus proteins. The capsaicin-containing feed additive and / or medicine can be used for preventing and treating fish virus diseases, and has the advantages of high protection efficiency and environmental friendliness.

The invention belongs to the technical field of fish genetic breeding, and particularly relates to a creation method of a crucian carp herpesvirus resistant fertile heterologous heptaploid crucian carp gynogenetic germline. The method comprises the following steps: firstly, breeding a gynogenesis double triploid prussian carp female parent and a sexual reproduction diploid megalobrama amblycephala male parent to obtain a filial generation G1, and then screening an individual of which the genome is fused with a set of megalobrama amblycephala sperm chromosome group from the filial generation G1, namely the heterologous heptaploid prussian carp which has the gynogenesis capacity. Cassius auratus gibelio herpesvirus infection experiments prove that the heterologous heptaploid Cassius auratus gibelio clone line has 100% anti-herpesvirus ability, and the breeding route provided by the invention can create a new Cassius auratus gibelio line with significant anti-herpesvirus ability and maintenance of gynogenesis ability, is an important way for breeding new Cassius auratus gibelio varieties, and has wide application prospects.

1. Name of the product in this design: Transmitter (H19 European Carp Fishing Alarm). 2. Purpose of this design: To trigger an alarm signal when a fish bites the hook while fishing. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The application discloses a method for forming fertilized eggs of Pseudobrama simus, and relates to the technical field of aquaculture, and comprises the following steps: step one, placing the fertilized eggs into a spray pipe; step two, evenly smearing the fertilized eggs by the water vapor sprayed by an adjusting mechanism and the stirring of a feather rod; step three, controlling the driving state of a driving mechanism by the adjusting mechanism to drive a piston mechanism to spray a large amount of water to flush the fertilized eggs into a hatching cylinder; and step four, adjusting the intensity by a fluctuation mechanism to make the fertilized eggs in a real water body fluctuation environment; the lower piston cylinder and the upper cavity of the double-cavity piston cylinder are controlled to perform piston movement to spray a large amount of gas and a small amount of water, then the water vapor is sprayed to the fertilized eggs in the form of water vapor through the spray pipe, and the blown fertilized eggs are uniformly arranged under the fluctuation of the feather rod; the application can control the fluctuation amplitude and make the fertilized eggs uniformly distributed on the supporting plate, so that the application can avoid the damage of eggshells or the injury of eggs caused by excessive pressure or excessive friction.

This application discloses a method for periodically feeding vine tea ultrafine powder in aquaculture and its application. The method includes the following steps: mixing vine tea ultrafine powder into aquatic animal feed at an addition rate of 1% to 8% of the total feed weight, and feeding it using a periodic feeding pattern; the periodic feeding pattern consists of continuous feeding for 7 days, followed by a 7-21 day break, which constitutes one cycle. This application, by pulverizing vine tea to an optimal particle size of 300 mesh, and by optimizing the optimal addition rate of 4% and the periodic feeding strategy of "7 days of continuous use, 14 days of break," fully utilizes the antioxidant and immune-enhancing effects of vine tea while effectively avoiding the potential liver and kidney burden and immunosuppression risks associated with long-term continuous use. Large-scale application trials show that this method achieves a protection rate of 52.91% to 65.14% in various aquatic animals, including crucian carp, largemouth bass, whiteleg shrimp, carp, and tilapia, demonstrating significant economic benefits and promising prospects for industrial application.

1. The name of this design product: Pot (Yellow River Carp). 2. Purpose of this design product: used for cooking food. 3. The key design point of this design product lies in the combination of shape and pattern. 4. The picture or photo that best illustrates the key points of the design: main view.

The invention relates to the technical field of cultivation, and discloses a gymnocyprinus carpio cultivation device which comprises a cultivation pool body, a plurality of sets of programmable water power assemblies are arranged in the cultivation pool body and communicate with a circulating water assembly located outside the cultivation pool body, and the circulating water assembly is communicated with the programmable water power assemblies. The outer wall of the water inlet of the cultivation pool body is sleeved with a non-thermal physical disinfection module. Through the programmable water power assembly, free switching of various flow states such as bottom layer washing laminar flow, whole pool suspension micro-circulation flow and pulse stimulation flow can be achieved in one cultivation pool body. In the spawning period, strong jet flow simulates the scouring environment of a natural riverbed, and the parent fish is effectively stimulated to ovulate; in the mouth opening period, special micro-circulation enables mouth opening bait to suspend through fluid lift force, and the problems that in traditional breeding, bait sinks to the bottom, and juvenile fishes are starved to death are solved; in the ripening accelerating period, the pulse water flow provides necessary movement load.

1. Name of the designed product: slippers (brocade carp). 2. Use of the designed product: slippers for wearing. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view.

1. Name of the designed product: fishhook (reverse bottom eel fishing set) 2. Use of the designed product: the designed product is used for fishing tools. 3. Design points of the designed product: the overall shape of the product. 4. Picture or photo that best indicates the design points: design 1 perspective view. 5. Design 1 is designated as the basic design.

The application discloses a molecular marker related to a high-sugar feed utilization character of Procyprinus, and application thereof. The molecular marker is located on the 7th chromosome of the Procyprinus, and contains one SNP site slc25a11-1 on the molecular marker. The SNP site slc25a11-1 is located at the 1439370th base of the 6th chromosome of the Procyprinus, and the polymorphic site is T / C. The high-sugar feed utilization of the Procyprinus species can be obviously improved through the molecular marker, so that the feed cost is reduced and the breeding benefit is increased.

The present invention provides a method for detecting and recognizing koi fish using multi-scale feature extraction, belonging to the technical field of deep learning image detection, and particularly relates to a method for detecting and recognizing the quality of koi fish. It is characterized by steps such as a data collection process, a picture processing and feature extraction process, multi-scale feature extraction, a training process, etc. The purpose is to improve the accuracy of koi fish recognition; the method used in the present invention has characteristics such as a high detection rate and a high accuracy rate when applied to underwater koi fish detection, making up for a series of problems generated in the traditional manual screening of koi fish, such as a low manual detection rate over time; it can ensure that the data set is strictly unified in format overall; it can solve the problem of insufficient koi fish data sets nowadays; it can effectively fuse the feature map information of different scales, further increase the detection rate of underwater koi fish, and can detect underwater koi fish targets with multiple occlusions.

This invention belongs to the field of aquaculture technology and relates to a novel use of capsaicin, particularly its use in the preparation of drugs and / or feed additives for the prevention and treatment of fish viral infections. The core of this invention lies in the significant protective effect of capsaicin against carp spring viremia virus (SVCV), grass carp reovirus (GCRV), and carp herpesvirus type 2 (CyHV-2) infections, effectively inhibiting the transcription of SVCV, GCRV, and CyHV-2 viral genes and the expression of viral proteins. Feed additives and / or drugs containing capsaicin can be used for the prevention and treatment of fish viral diseases, offering advantages such as high protective efficiency and environmental friendliness.

1. Name of the designed product: refrigerator sticker (fish jumping over the dragon gate). 2. Use of the designed product: for decorating refrigerator. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo best indicating the design points: perspective view 1.