425 results about "Protein molecules" patented technology

The invention provides a drug target binding affinity prediction method based on multi-modal data fusion. The method comprises the following steps: firstly, extracting sequence feature information of drug SMILES and target FASTA, then constructing an affinity graph, modeling drug molecules and target protein molecules into an undirected graph, and extracting molecular-level features of atoms, bonds, residues and contact. And fusing the hierarchical graph structure information of the affinity graph and the molecular graph to obtain the graph structure feature representation of the drug-target spot. The sequence feature information and the graph structure feature representation are further fused by using intramolecular and intermolecular attention fusion mechanisms. And finally, performing affinity prediction by using the fused features, and outputting a drug-target binding affinity score. According to the method, sequence and structural information are effectively fused, the accuracy of drug target affinity prediction is improved, and the problems of insufficient information fusion and insufficient structural information utilization in an existing method are solved.

The present invention provides a phycocyanin-collagen composite hydrogel and its preparation method and application. The present invention utilizes transglutaminase to catalyze the combination of phycocyanin and collagen to form a new, more stable collagen; transglutaminase acts between protein molecules, utilizes lysine and glutamic acid in phycocyanin and collagen to transfer acyl groups, and cross-links to form new chemical bonds, thereby enhancing the binding strength of phycocyanin and collagen; the hydrogel prepared by the present invention can be used to prepare medical materials and combined with skin injury sites to solve the problem that traditional dressings are easy to adhere to wounds and easily penetrated by tissue fluid and become infected. In addition to ensuring basic air permeability, it reduces the adhesion of the dressing, increases moisture retention and antioxidant properties, and avoids secondary damage when replacing a new dressing. The hydrogel prepared by the present invention has a certain antioxidant capacity and can be used to prepare products with antioxidant effects.

The invention provides a nylon 6 nanofiber virus-removing filtering membrane and a preparation method and application thereof.The nylon 6 nanofiber virus-removing filtering membrane comprises a base material and a nylon 6 nanofiber membrane compounded to the surface of the base material, the average pore size of the virus-removing filtering membrane is 20 nm, the virus-removing filtering membrane plays a role in blocking parvoviruses, the surface of the virus-removing filtering membrane is charged, and the surface of the virus-removing filtering membrane is not prone to being damaged. Proteins with the same charges on the surface can be prevented from adhering to the surface of the fiber. In the preparation process of the virus-removing filtering membrane, the nylon 6 nanofibers with a certain diameter range are selected and compounded on the surface of the base material in a stable dispersion state, the uniform microporous virus-removing filtering membrane is prepared, the effect that parvoviruses are blocked and protein molecules are not easily intercepted is achieved, the hydrophilicity of the nylon 6 nanofibers is relatively good, and the virus-removing filtering membrane has a good application prospect. The non-specific adsorption of the filtering membrane on the protein is reduced; the nylon 6 nanofiber virus-removing filtering membrane can be applied to the field of biological medicine products, and has important significance on separation and purification of biological preparations.

The invention provides an organic molecule cytoplasm delivery carrier as well as a preparation method and application thereof, the compound for cytoplasm delivery has the characteristic of fat-soluble aggregation, and can form electrostatic adsorption with small molecule protein goods such as RNase A (13.7 KDa), Trypsin (24KDa) and Saporin (32.8 KDa), medium molecule protein goods such as HRP (40KDa) and macromolecular protein goods such as beta-Gal (430KDa), so that the compound for cytoplasm delivery can be used for preparing a lipid-soluble protein carrier. And furthermore, transmembrane transportation is formed through the strong fat solubility property, so that the protection and intracellular delivery of carried protein molecules are realized. According to the delivery mechanism, cargo molecules can quickly penetrate through cell membranes, and carried protein molecules can enter cytoplasm to exert functions without release operation. Development of the organic molecule cytoplasm delivery carrier is expected to assist potential protein therapies with low bioavailability and off-target toxicity to complete clinical transformation, which is crucial to tumor therapy, gene therapy and the like requiring precise local administration.

The invention provides a monkey pox virus multi-antigen monovalent vaccine and application thereof. Specifically, the invention provides a monkey pox virus monovalent multi-antigen fusion protein, and the fusion protein comprises a first fusion protein element containing A35R and M1R proteins and a second fusion protein element containing A29L and B6R proteins from an N terminal to a C terminal. According to the invention, A35R, M1R, A29L and B6R are subjected to reasonable antigen combination to design a protein molecule, and the protein molecule is prepared into a monovalent mRNA vaccine. Experimental results show that the mRNA vaccine provided by the invention can ensure high-efficiency expression of target protein in vivo and can induce a high-level neutralizing antibody.

The invention provides a plug-and-play Mi3 protein nanocage fusion protein with a VNP6 tag and a preparation method and application of the plug-and-play Mi3 protein nanocage fusion protein, the fusion protein comprises the VNP6 tag, a SpyCatch003 component, a Mi3 protein nanocage and a functional protein sequence, and the VNP6 tag is located at the N end of the fusion protein. The VNP6 peptide sequence or the variant thereof is utilized to induce a vesicle or vesicle-like structure beneficial to protein folding and stabilization in escherichia coli, so that the correct folding rate and activity of recombinant protein are improved while the overall intracellular molecular crowding effect is relieved. The core idea of the invention lies in that the internal structure of cells is regulated at low temperature, and the internal microenvironment of an escherichia coli expression system is optimized, so that protein molecules under high expression load can obtain more reasonable spatial distribution, and the problems of misfolding and aggregation caused by molecular crowding are reduced.

The invention provides an oxygen-carrying protein molecule and a preparation method and application thereof, and relates to the technical field of functional protein preparation, the oxygen-carrying protein molecule comprises at least one cysteine part and at least one histidine part, the cysteine part comprises acetyl or ethyl maleoyl, and the histidine part comprises histidine. The histidine moiety includes a benzimidazole derivative group. The benzimidazole derivative is adopted as an auxiliary molecule and participates in the modification process of the sulfydryl modified compound on the hemoglobin, the modification site selectivity is improved, the conformational change is reduced, and the modification degree is accurately controlled, so that the oxygen binding force P50 of the modified hemoglobin is effectively improved.

The invention discloses a dextran-based HRP-goat anti-mouse IgG labeled antibody and a preparation method thereof, and the preparation method is characterized by comprising the following steps: S1, oxidizing dextran to obtain aldehyde group-containing dextran; s2, carrying out polymerization on the dextran containing the aldehyde group and horse radish peroxidase, so as to obtain a horse radish peroxidase polymer; s3, performing desalination and ultrafiltration on the horse radish peroxidase polymer to obtain an ultrafiltration product I; s4, connecting a goat anti-mouse IgG antibody to the ultrafiltration product I to obtain oxidized glucan coupled with goat anti-mouse IgG and HRP enzyme; and S5, purifying the oxidized dextran of the product coupled with the goat anti-mouse IgG and the HRP enzyme, so as to obtain the dextran coupled HRP-goat anti-mouse IgG labeled antibody. The dextran product obtained by the invention is wide in molecular weight coverage, different molecular weights can be selected to design polymer substances with different sizes, the number of aldehyde groups can be controlled to control coupling of different numbers of protein molecules under the same molecular weight, and the signal intensity is easy to control.

The invention discloses a potentiometric OECT biosensor as well as a preparation method and application thereof, and belongs to the technical field of organic electrochemical sensing. The potentiometric OECT biosensor comprises a device module and a sensing module, the device module comprises a substrate, and a source electrode, a grid electrode and a first floating gate are arranged on the substrate; a semiconductor channel is arranged on the source electrode, and a drain electrode is arranged on the semiconductor channel; a grid control grid is arranged on the grid; a hydrogel electrolyte layer is arranged at the tops of the substrate, the drain electrode, the grid-control grid electrode and the first floating gate; the sensing module adopts an electrode plate; the electrode plate comprises a gold electrode, an Ag / AgCl electrode and a carbon electrode; the gold electrode serves as a sensing grid electrode, an SAM connecting layer, targeted protein molecules and a shielding layer are arranged on the gold electrode, and when the biosensor works, an electrolyte layer is arranged at the top of the sensing module, and the Ag / AgCl electrode serves as a second floating grid; the first floating gate and the second floating gate are connected through a wire. The potentiometric OECT biosensor is high in detection sensitivity and high in response speed, and can meet clinical requirements on rapid and accurate detection.

In one aspect, a method is provided for identifying a residue in a particular conformation of a protein molecule that is capable of forming a stable bond between a compound and the protein molecule. The method can include identifying one or more residues from the conformation of the protein molecule to include in a perturbation set, the one or more residues exhibiting a protection factor that satisfies one or more thresholds. One or more residues may be excluded from the perturbation set based at least on the spatial arrangement and / or orientation of the one or more residues. One or more rounds of molecular dynamics simulations may be performed, the results of which are indicative of interactions between the compound and the residues in the perturbation set. The residues in the perturbation set may be identified as forming a stable binding between the compound and the protein molecule based at least on the results of the molecular dynamics simulation.

The invention belongs to the technical field of bioengineering, and particularly relates to an IL-12-CD137 chimeric protein, a function-enhanced engineered NK cell, and preparation and application thereof. The IL-12-CD137 chimeric protein comprises a signal peptide, an IL-12a protein molecule, a CD137 transmembrane structural domain and a CD137 intracellular structural domain, and has an amino acid sequence as shown in SEQ ID NO: 1. According to the function-enhanced engineered NK cell, the IL-12-CD137 chimeric protein sequence is introduced into an original NK cell, the function-enhanced engineered NK cell expressing Chime-IL12-CD137 is obtained, the amplification capacity and killing capacity of the NK cell can be enhanced, and the function-enhanced engineered NK cell is applied to treatment of tumor and virus-infected diseases of companion animals for the first time.

The invention relates to a prediction model for lung squamous cell carcinoma, and belongs to the technical field of disease prediction. On the basis of proteomics, protein molecule changes with specific execution functions in the lung squamous cell carcinoma occurrence process are screened, and a lung squamous cell carcinoma occurrence prediction model is constructed in combination with multiple machine learning models and public data. The prediction model for lung squamous cell carcinoma occurrence comprises six prediction factors: DDAH1, ATP11A, NME3, PON2, SLC1A5 and LPCAT1, and the prediction effectiveness (AUC value) of the prediction model can reach 97.6% and is higher than the prediction AUC value of a single factor.

The invention relates to the technical field of preparation of solid beverages, discloses a preparation method of a ketogenic solid beverage, and provides active high-protein cream powder, protein is modified through hydroxypropyl distarch sodium phosphate under the action of thermal induction and is covalently grafted with fructo-oligosaccharide, a structure with relatively high stability is formed after grafting, and the ketogenic solid beverage is prepared. And protein molecules are not easy to gather together. According to the present invention, the nutrition level of the product is further enriched, the absorption of the nutrition of each component by the human body is easily achieved, the malnutrition problem caused by the ketogenic diet is avoided, the unpleasant taste caused by the addition of fat and protein is avoided through the reasonable matching of the components, and the brewing property of the solid beverage is improved through the improvement of the active high-protein cream powder; the obtained ketogenic solid beverage has the characteristics of balanced nutrition, good flavor, stable property and easiness in storage.

The invention relates to a preparation method of a high-performance soybean protein emulsion film, which comprises the step of carrying out fibrosis denaturation on soybean protein by adopting an acetic acid-water binary solution, so that the hydrophobic property, the film-forming ability and the environmental interference resistance of the soybean protein can be remarkably improved. And further starting from a protein self-assembly fibrosis principle and a protein film forming theory, a soybean protein-based biological membrane with certain mechanical strength and flexibility is constructed through self-assembly fibrosis denaturation of protein molecules in an aqueous solution, so that the problems of poor mechanical property and high solubility of the existing protein-based biological membrane are effectively solved.

The invention discloses an anti-hardening protein bar suitable for cold conditions and a preparation method thereof, and belongs to the technical field of food processing. In order to solve the problems of texture hardening and mouthfeel deterioration caused by aggregation and crosslinking of protein molecules in the existing protein bar under a cold condition, the invention provides a method for organically combining specific plant proteins (soybean protein, quinoa protein, winter rapeseed protein and pea protein) rich in antifreeze peptide with antifreeze polysaccharide; the protein bar which can effectively resist hardening and keep good texture and nutritional characteristics in a cold environment is prepared through the processes of extrusion puffing, precise mixing, forming and the like, so that the application of the product in the fields of exercise and fitness, outdoor exploration, emergency storage and the like is expanded, and the market competitiveness of the protein bar is enhanced.

The invention relates to a therapeutic combination, comprising a first proteinaceous molecule comprising a first binding site for binding to a first epitope of a first cell-surface molecule, the first proteinaceous molecule provided with at least one saponin covalently bound to an amino-acid residue of said first proteinaceous molecule, and comprising a second pharmaceutical composition comprising a second proteinaceous molecule different from the first proteinaceous molecule, the second proteinaceous molecule comprising a second binding site for binding to a second epitope of a second cell-surface molecule different from the first cell-surface molecule, and comprising an effector moiety, wherein the second epitope is different from the first epitope. An aspect of the invention is a composition comprising the first proteinaceous molecule and the second proteinaceous molecule of the invention. The invention also relates to an antibody-drug conjugate comprising the first proteinaceous molecule of the invention and an effector moiety. An aspect of the invention relates to a pharmaceutical composition comprising the composition or the antibody-drug conjugate of the invention, and optionally further comprising a pharmaceutically acceptable excipient. The invention also relates to the therapeutic combination or the composition or the antibody-drug conjugate or the pharmaceutical composition of the invention, for use as a medicament. The invention also relates to the therapeutic combination of the invention for use in the treatment or prophylaxis of a cancer.

The invention provides a preparation method and application of a compound protein preparation, and the preparation method comprises the following steps: dissolving a protein matrix in deionized water, adjusting the pH value to 8.0-8.5, firstly adding an antioxidant, and then adding an oxidant and a reducing agent to obtain a protein coupling solution; dissolving the whey protein isolate in deionized water, adding a thickening agent, stirring, and cooling to room temperature to obtain a proteoglycan composite water phase; and mixing the protein coupling solution and the proteoglycan composite water phase according to a preset proportion, adding the oil phase, carrying out homogenization treatment to form a high internal phase emulsion, carrying out freeze drying, and crushing into particles to obtain the powdery protein pre-preparation. The structural stability of protein molecules is remarkably improved, and protein aggregation and sedimentation are reduced, so that the physical and chemical stability of the protein preparation in the storage and use processes is effectively improved, and the problem of insufficient structural stability in the prior art is solved.

The invention discloses a quenching type supramolecular fluorescent probe for pepsin detection as well as a preparation method and application of the quenching type supramolecular fluorescent probe. The quenching type supramolecular fluorescent probe is obtained by virtue of supramolecular interaction of a luminous monomer and a protein molecule; wherein the protein molecule is selected from any one or a combination of more of human serum albumin, bovine serum albumin, casein, hemoglobin and collagen; the luminous monomer comprises an alpha-cyano styrene derivative. The quenching type supramolecular fluorescent probe is easy to synthesize, has good water solubility, can be used as a quenching type fluorescent probe of pepsin, has good selectivity and anti-interference capability, and can well meet the requirements of high sensitivity and accurate detection of pepsin.

The invention discloses an enzyme-labeled secondary antibody polymer for immunohistochemical staining. The enzyme-labeled secondary antibody polymer is formed by combining a secondary antibody and a polymer skeleton, the polymer is labeled by a labeling enzyme, the polymer skeleton is polymethylacrylic acid-2-hydroxyethyl ester, and the labeling enzyme is connected to the polymethylacrylic acid-2-hydroxyethyl ester through a coupling agent. According to the invention, polymethylacrylic acid-2-hydroxyethyl ester is used for modifying protein, so that the immunogenicity and antigenicity are reduced, the hydrolysis of protease can be weakened, and the solubility of protein molecules is improved. The enzyme-labeled secondary antibody polymer is applied to immunohistochemical staining and has high specificity and sensitivity.

The invention discloses a compound water retention agent for food and a preparation method of the compound water retention agent, and mainly relates to the technical field of food additives. Compared with the prior art, sodium tripolyphosphate and sodium dihydrogen phosphate are used as composite phosphates, and are matched with the coordination effect of the enzyme preparation, glycerol, microcapsules, sodium carbonate, sodium citrate, maltodextrin, protein additive and thickening agent to maintain high water-retaining property, elasticity and tenderness. According to the invention, microcapsules prepared by taking glucomannan and the like as wall materials, taking casein as an emulsifier and taking grease core materials are also added, when the meat food is subjected to deep-frying processing, grease slowly released by the microcapsules can permeate into a meat protein network to weaken the cross-linking strength among protein molecules, and other components such as xanthan gum, guar gum and the like are matched, so that the meat food has a good deep-frying effect. Therefore, the hardness of the meat product is reduced, and high elasticity and tenderness are kept.

The utility model relates to the field of waste recycling equipment, in particular to a system for producing a liquid fertilizer by using fermentation waste liquid. The system comprises: a heat exchanger for heating the fermentation waste liquid; the separation part comprises a centrifugal machine and a reverse osmosis membrane, a feed port of the centrifugal machine is communicated with a discharge port of the heat exchanger, a feed port of the reverse osmosis membrane is communicated with a discharge port of the centrifugal machine, and the reverse osmosis membrane comprises a concentrated phase discharge port; a material inlet of the mixing treatment part is respectively communicated with a material outlet of the centrifugal machine and a concentrated phase material outlet; according to the system, a large amount of high-value liquid fertilizer can be obtained only through the heat exchanger, the separation part and the mixing treatment part, the operation steps and the treatment cost of the system are reduced, in addition, precipitation of large protein molecules in organic matter is achieved through the heat exchanger and the centrifugal machine, and the overall loss of raw materials is low.

The invention discloses a gallbladder malignant tumor protein diagnosis biomarker combination, a kit and application. The protein marker provided by the invention comprises CA1 and / or IGFBP6 (insulin-like growth factor binding protein 6). According to the invention, an LC-MS / MS mass spectrometry method is adopted, mass spectrometry is carried out on a large number of clinical plasma samples, and by calculating log2 difference multiples of corresponding protein molecule contents in the plasma of a gallbladder cancer patient and the plasma of a normal person, it is determined that two protein molecules (CA1 protein and IGFBP6 protein) have good inspection benefits; the kit can be used as a marker for early diagnosis of gallbladder cancer; therefore, the invention provides a non-invasive screening means based on plasma, and the non-invasive screening means is used for predicting the cholecystic malignant tumor and distinguishing the cholecystic carcinoma crowd from the non-cancer crowd. And materials are convenient to take, the blood sample amount is small, and the sensitivity is high. The invention can fill the blank that no protein marker combination with high diagnostic efficiency exists at present for the cholecystic malignant tumor, and has great clinical significance.

The invention relates to the technical field of protein detection, and discloses a protein sequencing method based on a nano motion platform and a nanopore, which comprises the following steps: step 1, processing a nano through hole, step 2, constructing a detection platform, step 3, electrically driving and embedding a functional protein into a solid nanopore, and detecting the protein by adopting a protein sequencing system. According to the protein sequencing method based on the nano-motion platform and the nano-pore, the distance between the driving protein in the maintaining device and the reading protein in the chip film is adjusted by using the sub-nano single-molecule control system, and the problem that the length reading is difficult due to the limitation of a nano-space during protein molecule detection at present is solved.

The invention provides an ischemic stroke auxiliary diagnosis system based on a peripheral blood T cell single cell transcriptome and application thereof, and the auxiliary diagnosis system comprises a peripheral blood mononuclear cell acquisition module, a single cell RNA sequencing module, a data processing module, a data analysis module and a result discrimination module. With a coding gene of a protein molecule significantly related to the occurrence of ischemic stroke as a target gene, a data processing module obtains the expression level of the target gene in each T cell; the data analysis module obtains a risk score of each T cell of the subject by using a built-in single cell risk scoring model, and weights the risk scores of all the T cells through distributed perception identification to obtain an individual risk score of the subject; and the result judgment module is used for classifying the subjects into ischemic stroke negative and ischemic stroke positive according to the individualized risk scores of the subjects. The auxiliary diagnosis system disclosed by the invention is high in ischemic stroke discrimination capability, and high in sensitivity and specificity.

The production equipment comprises a bottom plate, a collecting tank is fixedly connected to the position, close to the center, of the top of the bottom plate, a mixing barrel is arranged in an inner cavity of the collecting tank, a plurality of screening holes are formed in the bottom of the mixing barrel, a column body movably penetrates through the bottom of the mixing barrel, and the screening holes are formed in the bottom of the column body. According to the production equipment for extracting the small molecule peptide by using the earthworm protein powder, when the motor of the device works, the screening barrel can be driven to shake up and down, and the mixing barrel can be driven to rotate forwards and backwards, so that two purposes are achieved at one time, screening of materials in the screening barrel is accelerated, and the efficiency of extracting the small molecule peptide by using the earthworm protein powder is improved; and meanwhile, when the mixing barrel drives the second stirring rod to rotate forwards and backwards, the first stirring rod and the second stirring rod which are arranged in a staggered mode shear each other, protein molecules are impacted and sheared, and the splitting decomposition requirement of small molecule peptide is met.

To provide formulations of protein molecules comprising iduronate 2-sulfatase.SOLUTION: Certain embodiments provide a pharmaceutical composition comprising a protein molecule comprising an ERT enzyme-Fc fusion polypeptide and a modified Fc polypeptide, a buffer, an isotonicity agent, a surfactant, and a stabilizer, wherein the pH of the pharmaceutical composition is about 5.5 to 7.0, and further provide methods of use thereof. In certain embodiments, the buffer is selected from the group consisting of phosphate buffer, acetate buffer, arginine buffer, and histidine buffer. In certain embodiments, the phosphate buffer is a sodium phosphate buffer or a potassium phosphate buffer.SELECTED DRAWING: None

The invention belongs to the technical field of composite membrane preparation, and particularly relates to a salmon skin gelatin composite membrane with high mechanical performance and a preparation method thereof. Salmon skin gelatin, a chitosan solution, absolute ethyl alcohol, glycerin, sorbitol, montmorillonite, silicon dioxide, anhydrous sodium sulfite and a genipin acetic acid aqueous solution are used as raw materials, salmon skin gelatin protein and chitosan have good compatibility, protein molecules are fully stretched in the heat treatment denaturation process, the water absorption hydraulic radius of the protein molecules is increased, and the water absorption rate of the protein molecules is increased. The molecules interact with each other to form a compact space gel net structure; glycerol and sorbitol enhance the strength and toughness of the composite film; montmorillonite and silicon dioxide are beneficial to improving the mechanical strength of the composite film; in addition, the genipin acetic acid aqueous solution is added to modify free amino groups on salmon skin gelatin protein molecules, so that the interaction force among molecules is improved. Therefore, after the raw materials are specifically combined, synergistic interaction is achieved, and the mechanical performance of the salmon skin gelatin composite film is improved.

According to the method for detecting the conductance of the protein molecules and the application of the method, asymmetric modification is carried out on the tunneling electrode pair and the protein molecules, so that the protein molecules can enter a tunneling area in a fixed posture and form asymmetric single-molecule junctions with the tunneling electrode pair, the activity of the protein molecules can be kept to the maximum extent, and the conductivity of the protein molecules is detected. Therefore, the device capable of stably detecting the conductance of the protein molecules for a long time can be constructed and obtained, and the device can be used for researching the dynamic conformation of the protein, screening small-molecule drugs targeting the protein molecules and analyzing the interaction relationship between the small-molecule drugs and the protein molecules. The tunneling electrode device and the real-time electrical measurement technology are used for achieving protein detection on the single molecule level, the conformation change process of protein such as biological enzyme and the action characteristics of the protein and small molecules are comprehensively described on the high time resolution scale, and the design and optimization efficiency and success rate of existing drugs are expected to be improved.

The invention provides an anti-TIGIT antibody and IL-12 fusion protein and application thereof, and specifically, the fusion protein comprises a first binding region having TIGIT binding activity, a second binding region having TIGIT binding activity, and a third binding region having TIGIT binding activity; and a second binding region, the second binding region having an IL-12 receptor binding activity. The fusion protein can be effectively combined with TIGIT and IL-12 receptor protein molecules, the anti-TIGIT antibody relieves an immunosuppressive signal of TIGIT, meanwhile, IL-12 can promote proliferation and maintenance of immune cells and enhance the anti-tumor immune function, and the fusion protein can effectively treat and / or prevent cancers and has good clinical application value.

The present application relates to a kind of enzyme egg yolk liquid and its biological production method, belong to egg product technical field, using including compound plant component such as green pricklyash, Pleurotus eryngii as fermentation substrate, and using Lactobacillus bulgaricus and lactobacillus to ferment, preparation obtains compound plant enzyme, fermentation product can effectively inhibit the activity of oxidase, reduce the oxidative decomposition of lipid, maintain the quality and nutritional value of egg yolk liquid.Pretreated egg yolk liquid is mixed and is subjected to heat treatment, and enzyme egg yolk liquid is prepared.In the process of heat treatment, the protein in egg yolk liquid, including apo-protein, can be decomposed, the interaction between protein molecules is reduced, the formation of gel structure after egg yolk liquid freeze-thaw is reduced, and the bubble characteristics and taste are restored.