196 results about "Protein molecules" patented technology

The invention provides a nylon 6 nanofiber virus-removing filtering membrane and a preparation method and application thereof.The nylon 6 nanofiber virus-removing filtering membrane comprises a base material and a nylon 6 nanofiber membrane compounded to the surface of the base material, the average pore size of the virus-removing filtering membrane is 20 nm, the virus-removing filtering membrane plays a role in blocking parvoviruses, the surface of the virus-removing filtering membrane is charged, and the surface of the virus-removing filtering membrane is not prone to being damaged. Proteins with the same charges on the surface can be prevented from adhering to the surface of the fiber. In the preparation process of the virus-removing filtering membrane, the nylon 6 nanofibers with a certain diameter range are selected and compounded on the surface of the base material in a stable dispersion state, the uniform microporous virus-removing filtering membrane is prepared, the effect that parvoviruses are blocked and protein molecules are not easily intercepted is achieved, the hydrophilicity of the nylon 6 nanofibers is relatively good, and the virus-removing filtering membrane has a good application prospect. The non-specific adsorption of the filtering membrane on the protein is reduced; the nylon 6 nanofiber virus-removing filtering membrane can be applied to the field of biological medicine products, and has important significance on separation and purification of biological preparations.

The invention discloses an anti-hardening protein bar suitable for cold conditions and a preparation method thereof, and belongs to the technical field of food processing. In order to solve the problems of texture hardening and mouthfeel deterioration caused by aggregation and crosslinking of protein molecules in the existing protein bar under a cold condition, the invention provides a method for organically combining specific plant proteins (soybean protein, quinoa protein, winter rapeseed protein and pea protein) rich in antifreeze peptide with antifreeze polysaccharide; the protein bar which can effectively resist hardening and keep good texture and nutritional characteristics in a cold environment is prepared through the processes of extrusion puffing, precise mixing, forming and the like, so that the application of the product in the fields of exercise and fitness, outdoor exploration, emergency storage and the like is expanded, and the market competitiveness of the protein bar is enhanced.

The invention provides a preparation method and application of a compound protein preparation, and the preparation method comprises the following steps: dissolving a protein matrix in deionized water, adjusting the pH value to 8.0-8.5, firstly adding an antioxidant, and then adding an oxidant and a reducing agent to obtain a protein coupling solution; dissolving the whey protein isolate in deionized water, adding a thickening agent, stirring, and cooling to room temperature to obtain a proteoglycan composite water phase; and mixing the protein coupling solution and the proteoglycan composite water phase according to a preset proportion, adding the oil phase, carrying out homogenization treatment to form a high internal phase emulsion, carrying out freeze drying, and crushing into particles to obtain the powdery protein pre-preparation. The structural stability of protein molecules is remarkably improved, and protein aggregation and sedimentation are reduced, so that the physical and chemical stability of the protein preparation in the storage and use processes is effectively improved, and the problem of insufficient structural stability in the prior art is solved.

The invention discloses a gallbladder malignant tumor protein diagnosis biomarker combination, a kit and application. The protein marker provided by the invention comprises CA1 and / or IGFBP6 (insulin-like growth factor binding protein 6). According to the invention, an LC-MS / MS mass spectrometry method is adopted, mass spectrometry is carried out on a large number of clinical plasma samples, and by calculating log2 difference multiples of corresponding protein molecule contents in the plasma of a gallbladder cancer patient and the plasma of a normal person, it is determined that two protein molecules (CA1 protein and IGFBP6 protein) have good inspection benefits; the kit can be used as a marker for early diagnosis of gallbladder cancer; therefore, the invention provides a non-invasive screening means based on plasma, and the non-invasive screening means is used for predicting the cholecystic malignant tumor and distinguishing the cholecystic carcinoma crowd from the non-cancer crowd. And materials are convenient to take, the blood sample amount is small, and the sensitivity is high. The invention can fill the blank that no protein marker combination with high diagnostic efficiency exists at present for the cholecystic malignant tumor, and has great clinical significance.

The invention provides an ischemic stroke auxiliary diagnosis system based on a peripheral blood T cell single cell transcriptome and application thereof, and the auxiliary diagnosis system comprises a peripheral blood mononuclear cell acquisition module, a single cell RNA sequencing module, a data processing module, a data analysis module and a result discrimination module. With a coding gene of a protein molecule significantly related to the occurrence of ischemic stroke as a target gene, a data processing module obtains the expression level of the target gene in each T cell; the data analysis module obtains a risk score of each T cell of the subject by using a built-in single cell risk scoring model, and weights the risk scores of all the T cells through distributed perception identification to obtain an individual risk score of the subject; and the result judgment module is used for classifying the subjects into ischemic stroke negative and ischemic stroke positive according to the individualized risk scores of the subjects. The auxiliary diagnosis system disclosed by the invention is high in ischemic stroke discrimination capability, and high in sensitivity and specificity.

The present application relates to a kind of enzyme egg yolk liquid and its biological production method, belong to egg product technical field, using including compound plant component such as green pricklyash, Pleurotus eryngii as fermentation substrate, and using Lactobacillus bulgaricus and lactobacillus to ferment, preparation obtains compound plant enzyme, fermentation product can effectively inhibit the activity of oxidase, reduce the oxidative decomposition of lipid, maintain the quality and nutritional value of egg yolk liquid.Pretreated egg yolk liquid is mixed and is subjected to heat treatment, and enzyme egg yolk liquid is prepared.In the process of heat treatment, the protein in egg yolk liquid, including apo-protein, can be decomposed, the interaction between protein molecules is reduced, the formation of gel structure after egg yolk liquid freeze-thaw is reduced, and the bubble characteristics and taste are restored.

The application belongs to the field of electrochemical biosensors, and more particularly relates to a protein molecule labeling method for constructing a high-performance electrochemical sensor. In the application, organic ligands on the surface of sulfide colloidal quantum dots are replaced with water-soluble ligands through liquid-phase ligand replacement, and then mixed with a carboxyl activator to activate the carboxyl groups of the water-soluble ligands, followed by uniform mixing with protein molecules, so that the protein molecules are effectively labeled on the quantum dots. The quantum dots prepared in the application, which have water-soluble ligands on the surface and are labeled with protein molecules, are coated on the working electrode of a planar three-electrode system through a one-step method, and after drying, an electrochemical biosensor with a wide detection range, high response sensitivity and strong anti-interference performance is prepared.

The invention relates to the technical field of cytobiology and biomedicine detection, and discloses a time sequence fluorescence tracing system based on a coupling detection lipid probe. Comprising an imaging detection module which is used for adding FM lipophilic styrene fluorescent dye in a culture environment, forming a membrane probe to observe the morphological change of a membrane and detect the formation of vesicles, and completing the complete time sequence tracing of the cell endocytosis process by adopting a content dyeing method; the deep learning module is used for carrying out deep learning on the obtained time sequence image, establishing a living cell imaging screening and image analysis system, and identifying protein molecules related to the target external vesicles; carrying out image description on the generation, transportation and fusion processes of the vesicles generated by the proteins in different stages before fusion of the outer vesicles and the cell membranes and after fusion and shearing; and the knock-down operation module is used for exploring the generation mode of the vesicle contents in the early endosome in combination with knockout and knock-down operations of the specific drug compound.

The present invention relates to novel compounds of formula (I), optionally conjugated to peptide or protein molecules, and the synthesis thereof. The invention also relates to the use of the novel compounds for coupling reactions with linkers and other molecules. The invention also relates to the use of the novel compounds in bio-orthogonal strain promoted cycloaddition (copper-free), i.e. Click reaction. (I).

A titanium dioxide (TiO2) composition and methods of use, the composition containing surface-modified doped TiO2 nanoparticles (sm-TiO2 NPs) disposed in a polymer matrix material, wherein each sm-TiO2 NP has an outer surface having a plurality of bifunctional linker molecules attached thereto and a plurality of protein molecules linked to the sm-TiO2 NP via the bifunctional linker molecules; and wherein the polymer matrix comprises a polymer precursor component.

The invention belongs to the field of feed protein processing, and particularly relates to a method for improving the bioavailability of animal feed protein through chemical enzymolysis. The method comprises the following steps: mixing a compound protein raw material with water to form a suspension; sodium tripolyphosphate is added for phosphorylation pretreatment, so that protein is subjected to specific modification; after the pH is adjusted to be acidic, pepsase and acid phosphatase are added at the same time for an enzymolysis reaction; and after the reaction is finished, performing enzyme deactivation and drying to obtain the modified feed protein. According to the method, a specific enzyme cutting site is created on a protein molecule through chemical phosphorylation, then directional hydrolysis is achieved through efficient recognition of pepsin on the site, and meanwhile dynamic regulation and control of the hydrolysis process are achieved by means of the dephosphorylation effect of acid phosphatase. The obtained product has the outstanding advantages of high content of small peptides, good digestive absorbability, low bitterness, strong biological activity and the like, and the bioavailability of feed protein is remarkably improved.

The application provides a protein composition for predicting the prognosis effect of metastatic hormone-sensitive prostate cancer and application thereof, and belongs to the technical field of prognosis evaluation.The protein composition for predicting the prognosis effect of metastatic hormone-sensitive prostate cancer comprises protein molecules for evaluating drug resistance of a patient and protein molecules for evaluating sensitivity of the patient; the protein molecules for evaluating drug resistance of the patient comprise MCM4, NT5E, LTBP4, IAH1, HP, DOCK2 and EEF1A2; and the protein molecules for evaluating sensitivity of the patient comprise TFF3, FABP5 and LAMP2.The application constructs a molecular typing of mHSPC based on the above protein composition, evaluates the sensitivity of a mHSPC patient to ADT treatment in advance, and thus realizes precise treatment.

The present technology relates to commercial-scale methods for purifying botulinum toxin compositions obtained from cell cultures. Purification methods according to the present disclosure are based on a series of filtration and chromatographic separation steps that produce a high-purity botulinum toxin composition, which comprises botulinum toxin protein molecules (˜150 kDa) in solution, which is free, essentially free, or substantially free of botulinum toxin complexes and animal products, and without precipitating or lyophilizing botulinum toxin protein molecules. The purification method according to the present disclosure uses no precipitation, lyophilization, or centrifugation steps, permitting production of highly pure, highly active, free botulinum toxin protein molecules (˜150 kDa) in solution, without the need for reconstitution by the end user.

A method may include identifying an input molecule. A structural representation indicative of a conformation of the input molecule may be generated. In some cases, the structural representation may be a canonical conformation signature or a multimodal representation encoding the three-dimensional structural features of the input molecule. An output molecule may be generated by applying a molecule design computation model to modify the amino acid residue sequence of the input molecule. The modifying of the ammino acid residue sequence may be guided by the structural representations of the input molecule in order to preserve the conformation of the input molecule. In some cases, one or more properties of the output molecule may be determined based on the structural representation of the output molecule. Related systems and computer program products are also provided.

This invention relates to a fusion protein, immunogenic composition, and recombinant vaccine for the prevention of Escherichia coli infection. The invention provides a novel target, PstS, for Escherichia coli vaccine design. Starting from the tertiary structure of PstS and YidR proteins, and combining immunoepitaxes, stable domains of PstS and YidR proteins and their variants are ultimately screened, leading to the construction of a fusion protein. Both the PstS monoantigen protein molecule and the PstS-YidR fusion protein molecule of this invention exhibit good immunogenicity, can reduce tissue lesions caused by Escherichia coli infection, and demonstrate high efficacy in preventing Escherichia coli infection, showing broad application prospects.

The application relates to a molecule generation method and device, a molecule design method and device, and an electronic device. The molecule generation method comprises the following steps: obtaining a protein molecule comprising a protein pocket conformation and at least one starting fragment conformation matched with the protein pocket conformation; setting a current starting fragment conformation in the at least one starting fragment conformation to a current matching position of the protein pocket conformation, and determining a growth direction based on a starting growth site of the current starting fragment conformation; obtaining a growth fragment conformation, wherein the growth fragment conformation comprises a connection site and a first growth site; connecting the starting growth site and the connection site in the growth direction to obtain a grown starting fragment conformation; taking the grown starting fragment conformation as the current starting fragment conformation; and repeating the last two steps until a stop growth condition is met. The application can make the generated molecule structure matched with the protein pocket.

The invention relates to the technical field of biology, in particular to an SERS probe for Mfn2 detection, a preparation method of the SERS probe, an SERS sensor and application of the SERS sensor. Based on a surface enhanced Raman spectroscopy (SERS) technology, a gold nanoparticle SERS probe coated with an antibody 1 and a silver nanoparticle SERS probe coated with an antibody 2 are constructed, a sandwich structure is formed through combination of the antibody 1, an antigen and the antibody 2, the structure effectively improves SERS hot spot distribution and Mfn2 detection sensitivity, and traditional measurement steps are simplified. The constructed biologically coded SERS probe can be used for measuring Mfn2 with different concentrations. Based on the prepared SERS sensor, the dynamic measurement of the expression of the single living cell Mfn2 in the process of electrical stimulation of dental pulp stem cell odontogenic differentiation is realized, and the SERS sensor has wide clinical application prospect and transformation value in the aspects of measurement of protein molecules in a single cell organelle and diagnosis of Mfn2 related diseases.

The application provides application of MTA1 which is highly expressed in glioma stem cells (GSC) in treatment of glioblastoma (GBM). The application firstly proposes that GSC in GBM highly expresses MTA1 protein molecules, the highly expressed MTA1 is positively correlated with poor prognosis of GBM patients, and also promotes the stemness, cell proliferation and spheroid formation ability of GSC. Targeting the highly expressed MTA1 in GSC can inhibit the malignant progression of GBM. The application provides a new target and inhibitor for targeted treatment of GBM.

Certain embodiments provide a pharmaceutical composition comprising: a protein molecule comprising an ERT enzyme-Fc fusion polypeptide and a modified Fc polypeptide; a buffer; an isotonicity agent; a surfactant; and a stabilizer; wherein the pH of the pharmaceutical composition is about 5.5 to 7.0, as well as methods of use thereof.

To provide a computer program, an information processor and an information processing method for screening a drug candidate compound at a low calculation cost.SOLUTION: The computer program causes a computer to execute processing of acquiring binding information between a protein molecule and a compound molecule obtained by molecular docking simulation, and generating an evaluation index for screening whether or not the compound molecule is a candidate compound interacting with the protein molecule as a target protein based on the acquired binding information.SELECTED DRAWING: Figure 1

The application provides a set of methods for constructing antigen-antibody complex mutual recognition interface descriptors (i.e., protein molecular fingerprints), which maximally present the structure and physicochemical characteristics of specific recognition by describing the binding interface of the three-dimensional structure of the antibody-antigen from both sides. Based on the protein molecular fingerprints generated by the set of methods, based on the specific interaction recognition rules of the antigen-antibody, the epitope prediction algorithm specific to the antibody and the virtual screening model of the antibody are designed, and the existing machine learning or deep learning tools are docked, so that the antigen epitope prediction based on the antibody and the high-throughput virtual screening of the antibody based on the specific epitope can be quickly realized.

Methods of using lipid binding protein molecules to treat a subject having or at risk of one or more conditions, such as sepsis (e.g., septic shock).

Provided is a protein having a hexamer structure in which two molecules of a heterotrimeric protein associate at the N-terminus side, wherein the heterotrimeric protein is composed of (1) a fibrinogen Aα chain deficient in fibrinopeptide A, (2) a fibrinogen Bβ chain deficient in fibrinopeptide B and losing the ability to bind to B knob, and (3) a fibrinogen γ chain losing the ability to bind to A knob. The protein of the present invention can be used as a carrier for imparting physiological activity to fibrin gel in a thrombin-independent manner.

This invention relates to the field of food processing technology and discloses a process for preparing plant-based beverages containing casein hydrolysate peptides. The process includes: preheating a base liquid containing casein hydrolysate peptides and anionic polysaccharides and pumping it into a Venturi channel; inducing polysaccharide volume expansion by utilizing the hydrostatic pressure drop in the venturi channel to construct an isolation layer; simultaneously aspirating a low-temperature plant polyphenol extract, using the temperature difference to form an adhesion interface on the surface of the isolation layer; after the mixed fluid enters the expansion section, using the compressive force generated by hydrostatic recovery to press the polyphenols into the polysaccharide gaps, constructing a composite physical isolation phase; and triggering polysaccharide thermal gelation crosslinking by heating to establish a physical barrier. This invention utilizes the microscopic encapsulation mechanism generated by the fluid pressure field and temperature gradient to effectively block contact between polyphenols and protein molecules, inhibit complexation precipitation under thermal shock, and ensure that the beverage system possesses excellent thermal stability and storage stability.

Presented herein is a recombinant OPCML-Fc fusion protein molecule and methods of its use in cancer treatment where the Fc portion of the fusion protein molecule is only the CH2CH3 portion and lacks the hinge region containing the disulfide bridges preventing dimerization of the Fc portion and optimizing dimerization of the OPCML the D1 domain.

The application discloses an immunological analysis method based on high-molecular amplification signal amplification and belongs to the technical field of biological medicines. The application combines protein-high-molecular conjugates with biological analysis together, and successfully develops a method for protein quantitative analysis. On the basis of taking silica microspheres as a solid-phase carrier material, target analysis antigen protein molecules are coated in advance, specific recognition reaction of antigen and antibody is utilized, antibody IgG-I macromolecules grafted with a photo initiator in advance are indirectly fixed on the surface of the microspheres, and a "competition" immunological detection method is constructed. The method is a rapid, simple, inexpensive and enzyme-free cascade amplification detection technology, and has a wide application prospect.

Methods of characterizing analytes such as protein molecules by differential association or dissociation of binding reagents are provided. Observation of association or dissociation of binding reagents from analytes under two or more differing association or dissociation conditions can facilitate characterization of the analytes due to observed differences in binding patterns.

The invention belongs to the technical field of protein, and provides a method for preparing earthworm peptide by adopting a deep-eutectic solvent in combination with an electric field effect, the method comprises the following steps: (1) mixing an earthworm protein raw material with the deep-eutectic solvent to form a uniform reaction system; (2) an external electric field with preset parameters is applied to the reaction system for a reaction, so that earthworm protein molecules are broken under the synergistic effect of the electric field and DES, and earthworm peptide is generated; and (3) after the reaction is finished, separating and purifying a reaction product to obtain the target earthworm peptide. The method is mild in condition, green, environment-friendly, short in reaction time and low in energy consumption, protease does not need to be added, and the molecular weight of the product can be controlled by adjusting parameters such as electric field intensity. The average molecular weight of the obtained earthworm peptide is lower than 1000 Da, the DES residual quantity is low, the peptide fragment composition is unique, the function of remarkably improving insulin resistance is achieved, and the earthworm peptide is superior to a product prepared through a traditional enzymolysis or acidolysis method and suitable for industrial large-scale production.

The application relates to a detection device for resolving proteins based on magnetophoresis and a detection method thereof, and relates to the technical field of protein abundance and molecular weight detection. The detection device comprises a magnetophoresis preparation part and a protein magnetophoresis part which are sequentially communicated. The magnetophoresis preparation part comprises a mixing container containing SDS solution, the SDS solution is used to eliminate the charge difference between protein molecules, a pipeline is connected to the outlet end of the mixing container, the pipeline sequentially passes through a stirring area provided with an ultrasonic stirrer and a uniform electric field area provided with a uniform electric field generator, the protein magnetophoresis part comprises a separation chamber arranged in the uniform magnetic field area, different protein molecules are separated according to the difference in molecular weight, and the separated protein molecules are detected by a detector, analyzed by an analysis device and displayed, and the relative sizes of the abundance and molecular weight of the proteins are reflected. The detection device has the advantages of simple structure, convenient operation and the like, and provides a new detection method for detecting the relative sizes of the abundance and molecular weight of proteins.

The invention discloses a probe set. The probe set comprises a first fusion protein and a second fusion protein, wherein the first fusion protein comprises a nuclear localization sequence and a first protein molecule, and the nuclear localization sequence is connected with the first protein molecule; the second fusion protein comprises a TFEB protein and a second protein molecule, the TFEB protein is connected with the second protein molecule, the second protein molecule is used for being combined with the first protein molecule, and a detectable signal is generated after the first protein molecule and the second protein molecule are combined. The probe set can rapidly, accurately and sensitively detect the TFEB nuclear transfer condition, can be used for high-throughput screening of TFEB regulation and control drugs, and reduces the drug screening cost.