35 results about "Protein molecules" patented technology

The present application relates to a kind of enzyme egg yolk liquid and its biological production method, belong to egg product technical field, using including compound plant component such as green pricklyash, Pleurotus eryngii as fermentation substrate, and using Lactobacillus bulgaricus and lactobacillus to ferment, preparation obtains compound plant enzyme, fermentation product can effectively inhibit the activity of oxidase, reduce the oxidative decomposition of lipid, maintain the quality and nutritional value of egg yolk liquid.Pretreated egg yolk liquid is mixed and is subjected to heat treatment, and enzyme egg yolk liquid is prepared.In the process of heat treatment, the protein in egg yolk liquid, including apo-protein, can be decomposed, the interaction between protein molecules is reduced, the formation of gel structure after egg yolk liquid freeze-thaw is reduced, and the bubble characteristics and taste are restored.

A titanium dioxide (TiO2) composition and methods of use, the composition containing surface-modified doped TiO2 nanoparticles (sm-TiO2 NPs) disposed in a polymer matrix material, wherein each sm-TiO2 NP has an outer surface having a plurality of bifunctional linker molecules attached thereto and a plurality of protein molecules linked to the sm-TiO2 NP via the bifunctional linker molecules; and wherein the polymer matrix comprises a polymer precursor component.

A method may include identifying an input molecule. A structural representation indicative of a conformation of the input molecule may be generated. In some cases, the structural representation may be a canonical conformation signature or a multimodal representation encoding the three-dimensional structural features of the input molecule. An output molecule may be generated by applying a molecule design computation model to modify the amino acid residue sequence of the input molecule. The modifying of the ammino acid residue sequence may be guided by the structural representations of the input molecule in order to preserve the conformation of the input molecule. In some cases, one or more properties of the output molecule may be determined based on the structural representation of the output molecule. Related systems and computer program products are also provided.

Certain embodiments provide a pharmaceutical composition comprising: a protein molecule comprising an ERT enzyme-Fc fusion polypeptide and a modified Fc polypeptide; a buffer; an isotonicity agent; a surfactant; and a stabilizer; wherein the pH of the pharmaceutical composition is about 5.5 to 7.0, as well as methods of use thereof.

This invention relates to the field of food processing technology and discloses a process for preparing plant-based beverages containing casein hydrolysate peptides. The process includes: preheating a base liquid containing casein hydrolysate peptides and anionic polysaccharides and pumping it into a Venturi channel; inducing polysaccharide volume expansion by utilizing the hydrostatic pressure drop in the venturi channel to construct an isolation layer; simultaneously aspirating a low-temperature plant polyphenol extract, using the temperature difference to form an adhesion interface on the surface of the isolation layer; after the mixed fluid enters the expansion section, using the compressive force generated by hydrostatic recovery to press the polyphenols into the polysaccharide gaps, constructing a composite physical isolation phase; and triggering polysaccharide thermal gelation crosslinking by heating to establish a physical barrier. This invention utilizes the microscopic encapsulation mechanism generated by the fluid pressure field and temperature gradient to effectively block contact between polyphenols and protein molecules, inhibit complexation precipitation under thermal shock, and ensure that the beverage system possesses excellent thermal stability and storage stability.

Presented herein is a recombinant OPCML-Fc fusion protein molecule and methods of its use in cancer treatment where the Fc portion of the fusion protein molecule is only the CH2CH3 portion and lacks the hinge region containing the disulfide bridges preventing dimerization of the Fc portion and optimizing dimerization of the OPCML the D1 domain.

The application relates to a detection device for resolving proteins based on magnetophoresis and a detection method thereof, and relates to the technical field of protein abundance and molecular weight detection. The detection device comprises a magnetophoresis preparation part and a protein magnetophoresis part which are sequentially communicated. The magnetophoresis preparation part comprises a mixing container containing SDS solution, the SDS solution is used to eliminate the charge difference between protein molecules, a pipeline is connected to the outlet end of the mixing container, the pipeline sequentially passes through a stirring area provided with an ultrasonic stirrer and a uniform electric field area provided with a uniform electric field generator, the protein magnetophoresis part comprises a separation chamber arranged in the uniform magnetic field area, different protein molecules are separated according to the difference in molecular weight, and the separated protein molecules are detected by a detector, analyzed by an analysis device and displayed, and the relative sizes of the abundance and molecular weight of the proteins are reflected. The detection device has the advantages of simple structure, convenient operation and the like, and provides a new detection method for detecting the relative sizes of the abundance and molecular weight of proteins.

The invention relates to a computer-implemented method performed by one or more data processing apparatus for assessing a biological feature of a biological macromolecule, preferably a protein molecule, by providing an electrostatic data based in silico representation of the biological macromolecule. The biological feature may be, without limitation, multiple function-related states of the macromolecule, differences in the conformation, e.g. in the secondary structure, patterns based on primary structure, folded nature of the protein including, among others, π-π relationships and relationship between the genetic code and the electrostatic state of the environment of the encoded amino acid(s). The invention can be used among others in the field of drug research, medical diagnosis, genetic engineering and macromolecular modeling.

The application discloses a kind of MS2 virus-like particles based on capsid protein site-directed mutation and its preparation method and application, belong to molecular biological technique field.The amino acid of the beta sheet interface of capsid protein sequence, the amino acid of the interface when two capsid proteins molecules assemble, the carboxyl end site of capsid protein is subjected to site-directed mutation, obtain the mutant that can significantly improve the assembly efficiency and stability of capsid protein dimer.Utilize the mutant to construct MS2 virus-like particles, further by the design of mature enzyme and capsid protein expression element, control mature enzyme weak expression, capsid protein strong expression, to improve assembly efficiency.The application improves the assembly efficiency of MS2 virus by the above strategy, improves the proportion of wrapped RNA, enhances the stability of virus-like particle, greatly prolongs the stable storage time of RNA, improves the purity of wrapped RNA.

This application relates to a method and apparatus for analyzing the rigidity weaknesses of protein structures based on an atomic node and network constraint model. The method includes: obtaining the first crystal structure of the target protein molecule; performing a free energy minimization simulation on the first crystal structure to obtain an energy-minimized crystal structure; constructing an atomic node and network constraint model of the energy-minimized crystal structure; performing pyrolysis and folding molecular dynamics simulations on the atomic node and network constraint model until the atomic node and network constraint model reaches a state of equilibrium; analyzing the atomic node and network constraint model in equilibrium and obtaining the analysis results; and identifying rigidity weak regions in the target protein molecule based on the analysis results. The scheme provided in this application can calculate the rigidity weaknesses of the protein structure, find more suitable modification sites or regions, and thus enable targeted mutation of the protein to improve the stability of the protein molecule.

The present application relates to the use of a substance that regulates BCL11A gene and / or its encoded product in the preparation of a drug for reversing skin photoaging, wherein the skin photoaging includes skin cell aging caused by sunlight, light, etc., and the skin cells include keratinocytes, dermal fibroblasts, melanocytes. The regulation is to up-regulate the expression of BCL11A gene and / or promote the production of its encoded product. Typical clinical manifestations of the skin photoaging include wrinkles, dry skin, loose skin, dilated capillaries, uneven skin pigmentation, and skin itching. The substance that regulates BCL11A gene and / or its encoded product includes a substance that regulates BCL11A gene DNA molecules and a substance that regulates the expression of BCL11A translation product protein molecules. The reversal of skin photoaging is manifested in reducing the proportion of cells positive for aging-related beta-galactosidase staining in skin cells and reducing the expression of aging-related protein p16 and DNA damage protein gammaH2AX.

The present application relates to an enzyme labeled plate and a preparation method thereof, and belongs to the technical field of enzyme labeled plates. The enzyme labeled plate comprises a solid carrier and a functional layer arranged on the surface of the solid carrier; the raw material of the solid carrier comprises aminated polystyrene and polystyrene; and the surface of the solid carrier is treated by hydrophilic modification to form the functional layer. Because the aminated polystyrene contains an amino active group, the surface activity of the enzyme labeled plate can be improved, then the surface of the solid carrier is treated by hydrophilic modification to form the functional layer, so that the surface of the enzyme labeled plate can produce strong adsorption and strong reaction activity with protein molecules such as antigens or antibodies, the effect of coating the enzyme labeled plate with protein molecules such as antigens or antibodies is improved, and the sensitivity of ELISA testing is improved.

This invention discloses a method for real-time monitoring of the inhibitory effect of a small molecule drug, vorinostat, comprising the following steps: constructing a fluorescence confocal electrochemical impedance microscopy imaging system using a WS2 chip as the working electrode; immobilizing probe protein molecules on the surface of the WS2 chip; applying an electrical signal to the WS2 chip after probe protein molecule immobilization, and monitoring the inhibitory effect of the small molecule drug by the change curve of the amplitude signal over time. In this invention, when the small molecule drug binds to the probe protein molecules immobilized on the WS2 surface, the surface charge of the WS2 changes, and the inhibitory effect of the small molecule drug on the probe protein molecules is monitored by the change of the WS2 fluorescence signal.

This invention belongs to the field of egg product processing technology, and discloses a Maillard reaction-based flavor-enhancing egg liquid, its preparation method, and its application. The flavor-enhancing egg liquid of this invention is prepared from the following raw material components via a Maillard reaction: egg liquid, L-cysteine, and natural compound sugars. This invention, for the first time, synergistically activates endogenous cysteine ​​proteases in egg liquid by adding L-cysteine, achieving precise and targeted hydrolysis of large protein molecules in the egg liquid without the introduction of exogenous proteases. This provides sufficient precursor substances for the Maillard reaction, thereby significantly enhancing the natural egg aroma, caramel aroma, and other characteristic flavors of the egg liquid. This flavor-enhancing egg liquid has a naturally rich aroma, high integration with the matrix, and maintains good emulsification and fluidity. When used in the production of baked goods such as cakes, bread, and egg tarts, it can impart a rich and lasting natural egg aroma to the final product, while also improving the color and taste of the final product, showing promising prospects for industrial application.

Disclosed are proteinaceous molecules that are useful for reducing axonal degeneration (e.g., SARM1-mediated axonal degeneration), inducing synapse formation, inhibiting or minimising neuronal injury and treating, or reducing the severity of, neurodegenerative diseases in subjects.

The present application relates to the application of SPEM3 protein or TEX50 protein in the microstructure display of the post-loop of sperm, and the preparation of a diagnostic agent for sperm deformity, and further relates to a knockout Spem3 or Tex50 mammalian male sterility model, and a sperm post-loop visualized animal model with a displayable label on the SPEM3 protein molecule or TEX50 protein molecule, and the application thereof. The present application discloses the post-loop localization of SPEM3 and TEX50 in mouse and human sperm, and the combination Spem3 – / – and Tex50 – / – of SPEM3 and TEX50 in the diagnosis and treatment of sperm deformity.

Methods of using a lipid binding protein molecule to treat a subject having one or more conditions or at risk of one or more conditions. The method generally includes measuring ApoA-I levels and / or HDL cholesterol levels in the subject, and administering one or more doses of the lipid binding protein molecule to the subject if the measured ApoA-I levels are below a target ApoA-I level or target ApoA-I range, and / or the measured HDL levels are below a target HDL level.

A T cell receptor targeting a KRAS G12V mutant polypeptide and a use thereof, wherein the T cell receptor molecule specifically targets a KRAS G12V mutation; a CDR3 sequence of an α chain variable domain thereof contains CAVRDIEGAGNNRKLIW (SEQ ID NO: 1) or a mutant of SEQ ID NO: 1, and / or a CDR3 sequence of a β chain variable domain contains CASSEGQYSYEQYF (SEQ ID NO: 2) or a mutant of SEQ ID NO: 2. Furthermore, a nucleic acid molecule encodes the T cell receptor molecule, a dual-targeting protein molecule and a multivalent complex includes the T cell receptor molecule, a nucleic acid construct, a cell expressing the T cell receptor molecule, etc.

This invention relates to a flavin-dependent monooxygenase mutant and its application in indigo production, belonging to the field of enzyme engineering technology. Based on a flavin-dependent monooxygenase derived from *Methvlophaga aminisulfidivorans*, this invention employs deep learning methods to modify protein molecules, obtaining multiple single- or multi-site mutants. Experimental verification of their catalytic effect on indigo synthesis yielded mutants with mutations at five specific amino acid sites: W319A, C78D, K198A, D197E, and Y320A. These mutants exhibit significantly enhanced catalytic activity compared to the wild type. Specifically, the three-site combination mutant K198A / D197E / Y320A showed a 2.58-fold increase in enzyme activity compared to the wild type. In indigo production, these mutants can effectively increase indigo yield.

Certain embodiments of the invention provide isolated anti-Ebola single domain antibodies (sdAbs), as well as polypeptides and protein molecules comprising such sdAbs, including Fc fusion thereof, and bispecific anti-Ebola binder protein. Certain embodiments of the invention also provide methods of using these sdAbs, polypeptides and protein molecules for treating an Ebola virus infection.

The application discloses a method for preparing salt and heat double-resistant soybean protein powder by using salt-containing preheating combined with micro-jet circulation treatment, and the method comprises the following steps: dispersing soybean protein powder in a phosphate buffer solution, preheating treatment after adding sodium chloride, and then micro-jet treatment; performing modification through circulation "preheating-micro-jet" combined treatment; and finally obtaining salt and heat double-resistant soybean protein powder through freeze drying. In the preheating stage, the method introduces sodium chloride, regulates the electrostatic interaction and hydration between protein molecules, reshapes the protein unfolding path, and promotes the cross-linking and recombination of the protein into controllable intermediate aggregates. Then, the protein is further broken and rearranged through the shearing, impact and cavity effect of high-pressure micro-jet, and the controllable aggregation and dynamic reconstruction are realized through multiple circulation, so that uniform and dense protein particles are finally formed. The soybean protein obtained by the method can still maintain good fluidity after being heated under high-protein concentration and high-salt conditions, and is suitable for salt-containing high-protein beverages and other related fields.

The present application relates to the technical field of food detection, in particular to a method for detecting the protein content in soy milk powder, comprising: obtaining the original near-infrared spectrum data of the soy milk powder sample to be detected and constructing a protein characteristic vector; calling a preset protein optical constant database storing the light absorption coefficient and refractive index under different protein concentrations, matching and then backstepping the average light path and extinction coefficient of the protein molecules to obtain light path extinction data; based on the data, establishing an absorption kinetics model to simulate the propagation process of light in the suspension and obtain the light intensity decay state, and after time integration, obtaining the cumulative absorbance proportional to the number of protein molecules; combining the dry weight of the sample to calculate the protein content per unit mass, and after environmental temperature compensation to correct the spectral drift, outputting the standardized detection result. The method can improve the accuracy and stability of the detection of the protein content in soy milk powder.

A casein hydrolysate-chlorogenic acid non-covalent complex emulsion as well as a preparation method and application thereof belong to the technical field of food processing. In view of the fact that the prior art cannot simultaneously consider the alpha-glucosidase inhibitory activity, emulsification stability and antioxidant activity of the casein hydrolysate, the casein hydrolysate-chlorogenic acid complex is prepared through non-covalent interaction, and the complex is used as an emulsifier to construct a functional emulsion. The interaction mainly occurs through hydrogen bonds and hydrophobic interaction, resulting in the unfolding of protein molecular structure and the fluorescence quenching. Meanwhile, the functional emulsion has good emulsification activity and stability. The emulsion prepared by using the complex has the smallest particle size, the lowest turbidity and uniform droplet distribution. The functional emulsion obtained by the application has the antidiabetic effect and excellent emulsification performance, and has important application value in the development of functional foods.

The application discloses a freeze-dried diluent as well as a preparation method and application thereof, and belongs to the technical field of biological detection. The freeze-dried diluent is added with a simple buffer system of a low-concentration inorganic acid (such as 1-5 mM hydrochloric acid), so that the stability problem of a recombinant protein (GDF-15) caused by high pI and strong hydrophobicity can be overcome in a targeted manner. The system provides a strong electrostatic repulsive force for protein molecules by providing an acidic environment, and fundamentally eliminates non-specific adsorption and salt-induced aggregation, so that high-activity recovery and long-term stability of a freeze-dried recombinant protein standard are realized. In addition, through synergistic action between the inorganic acid and other components (protein stabilizers, sugar compounds, metal ion chelating agents and preservatives) in the freeze-dried diluent, the attenuation of the recombinant protein standard can be effectively reduced, and the stability of the recombinant protein standard can be significantly improved, and then the long-term effectiveness of an ELISA kit and the detection accuracy can be significantly improved.

Provided are a PD-1 molecule for blocking the binding of an anti-PD-1 antibody to a PD-1 molecule on a cell surface, and a mutant and the use thereof. Further provided is a protein molecule, which has 80-100% sequence identity to a wild-type protein as shown in SEQ ID NO: 1. The provided recombinant protein H126 and H134 molecules can competitively bind to an anti-PD-1 antibody drug with the endogenous PD-1 molecule expressed by a T cell, without affecting the binding of PD-1 to an endogenous PD-L1 molecule, thereby restoring the function suppression on activated T cells and alleviating the symptoms of immunotherapy-related adverse events. By means of the protein molecule, a new way of performing clinical treatment of the immunotherapy-related adverse events is provided.

Provided herein are protein molecules that specifically bind PD-L1 and also exhibit activatable specific CD28 binding in diseased tissues. Further provided herein are uses of such protein molecules to treat cancer.

The application relates to a protein-molecule interaction prediction method, device, equipment and storage medium. The method comprises the following steps: performing feature extraction on protein sequence data and molecular structure data to obtain protein features and molecular structure features; performing cross-modal feature fusion on the protein features and the molecular structure features by using a protein-molecule cross-fusion module based on a Transformer, and outputting high-dimensional feature representation after fusion; performing model training and optimization based on the high-dimensional feature representation after fusion by using a teacher-student model architecture and a knowledge distillation technology to obtain a trained prediction model; and predicting the protein-molecule interaction intensity by using the trained prediction model. By combining cross-modal feature fusion and a teacher-student model architecture, the application can improve the ability to capture key information in protein and molecular structure, solve the limitation that a traditional method can only process single-modal data, and improve the prediction accuracy of a PDI task.

The application provides a heteromorphic bifunctional crosslinking agent and preparation and application thereof. The heteromorphic bifunctional crosslinking agent provided by the application is a bifunctional crosslinking agent containing N-hydroxysuccinimide (NHS) active ester and a dinitroimidazole functional group (DNIm), and can connect together a compound containing amino and a compound containing sulfydryl. The DNIm module of the crosslinking agent provided by the application is more stable than a maleimide module. In addition, the addition product of DNIm and sulfydryl is also more stable than the addition product of maleimide and sulfydryl. In a near neutral buffer, no side reaction product of the addition of DNIm and amino is found through LC-MS analysis, that is, no addition reaction with amino occurs under the condition. The crosslinking agent provided by the application can be applied to the construction of cyclic peptides of different sizes, and the selective single modification of sulfydryl or amino of proteins, including biotinylation, PEGylation or the introduction of fluorescent substances, and the double modification of a single protein molecule, including biotinylation and PEGylation.

This invention relates to the field of biodetection technology, providing an enzyme cascade reaction system for the detection of nucleic acids and protein molecules, comprising BER endonuclease and Argonaute endonuclease; wherein the BER endonuclease is one or more selected from APE1, FEN1, Fpg, UDG, and T7endonuclease, and the Argonaute endonuclease is one or more selected from pfAgo, CpAgo, CbAgo, TtAgo, RasAgo, MjAgo, and NgAgo. This invention also provides a method for detecting nucleic acids and protein molecules using the above system, and the application of the above system in the preparation of target nucleic acid and target protein molecule products from detection samples. This invention utilizes the synergistic enzyme activity of APE1 and PfAgo in the enzyme cascade reaction system to achieve real-time detection of nucleic acid and protein biomarkers without pre-amplification within 45 minutes.

This invention belongs to the field of reactive oxygen species (ROS) detection technology, and discloses a composite near-infrared ratiometric fluorescent probe, its synthesis method, and its application. The probe is composed of a cyanine organic fluorescent dye and bovine serum albumin (BSA). By using urea to disrupt the hydrogen bonds, the BSA protein molecule structure is relaxed and denatured, leading to the extension of the protein's peptide chains. These chains then bind to the synthesized organic compound Hcy-Cl to form the probe Hcy-BSA structure. The ROS level in vivo is detected by ratiometric fluorescence intensity changes. ‑ This invention designs a novel composite near-infrared ratiometric fluorescent probe, which achieves the regulation of ClO through changes in ratiometric fluorescence intensity. ‑ The detection method has advantages such as good selectivity, high stability, good biocompatibility and low toxicity, and is expected to become a powerful auxiliary reagent for the early diagnosis of tumors and Alzheimer's disease.