1367 results about "Protein molecules" patented technology

In accordance with the present invention, there are provided methods for the extension of serum half-lives of proteinaceous molecules, particularly antibody molecules, and compositions of molecules modified in accordance with the methods of the invention. In accordance with a first aspect of the present invention, there is provided a method of modifying the half-life of an antibody through providing an antibody containing an FcRn binding domain or the genes encoding such antibody and physically linking the antibody or the antibody as encoded to a second FcRn binding domain. In accordance with a second aspect of the present invention, there is provided a molecule that contains at least two distinct FcRn binding moieties.

The present invention provides combinations of specific binding proteins, such as immunoglobulins, that are designed to be true combinations, essentially all components of the combination being functional and compatible with each other. The invention further provides a method for producing a composition comprising at least two different proteinaceous molecules comprising paired variable regions, the at least two proteinaceous molecules having different binding specificities, comprising paired variable regions, at least two proteinaceous molecules having different binding specificities, comprising contacting at least three different variable regions under conditions allowing for pairing of variable regions and harvesting essentially all proteinaceous molecules having binding specificities resulting from the pairing.

The present invention provides combinations of specific binding proteins, such as immunoglobulins, that are designed to be true combinations, essentially all components of the combination being functional and compatible with each other. The invention further provides a method for producing a composition comprising at least two different proteinaceous molecules comprising paired variable regions, the at least two proteinaceous molecules having different binding specificities, comprising paired variable regions, at least two proteinaceous molecules having different binding specificities, comprising contacting at least three different variable regions under conditions allowing for pairing of variable regions and harvesting essentially all proteinaceous molecules having binding specificities resulting from the pairing.

The invention in suitable embodiments is directed to smart bio-nanoparticle elements and intelligent bio-nanoparticle platforms employing such smart bio-nanoparticle elements. In one aspect, the smart bio-nanoparticle elements are formed with self-assembling protein molecules.

The invention discloses a culture medium for culturing mesenchymal stem cells. The culture medium comprises improved minimum medium IMDM, cell growth factor combination, insulin, trace elements, lipid substances, vitamin substances, hormone substances, protein molecules, amino acid substances and other compounds. The culture medium provided by the invention does not contain serum or plasma (comprising animal or human serum or plasma), thus, when the system is used for culturing the mesenchymal stem cells, zoonosis caused by the animal serum or plasma in the cell therapy process can be avoided, and the risk of human immunity reaction which is possibly caused in the cell therapy process can be avoided. The culture medium has specific chemical constituent, thus the possibility of different cultured mesenchymal stem cell properties caused by the difference of culture systems can be avoided. The culture medium is not only suitable for culturing the mesenchymal stem cells of human marrow and umbilical cord resources but also suitable for culturing the mesenchymal stem cells of other tissues or other animals, and has the advantage of wide application range.

The invention discloses a SERS biological probe and a preparation method thereof. The SERS biological probe provided by the invention comprises a SiO2 core and a metal nanoparticle layer on the surface of the SiO2 core, wherein a plurality of Raman signal molecules connected with the metal nanoparticle layer; a SiO2 layer is also provided outside the metal nanoparticle layer; the Raman signal molecules are positioned between the metal nanoparticle layer and the SiO2 layer; the SiO2 layer surface is modified with biological probe molecules; and the metal nanoparticle layer is Au nanoparticle layer or Ag nanoparticle layer. The SERS biological probe provided by the invention has wide generality, and has wide application prospect in identification and detection of biological molecules (DNA molecules and protein molecules), rapid disease diagnosis, biomedical imaging technology as well as in the fields of serious disease treatment, food hygiene, environmental monitoring, etc.

Novel proteins and protein libraries are disclosed. The proteins possess one or more functional protein modules from different parent protein molecules. The proteins and protein libraries are exemplified by the preparation of cross-over chemokines that contain various combinations of peptide segments derived from RANTES, SDS-1 and vMIP-I and to vMIP-II. The proteins and libraries are extremely pure and can be provided in non-limiting high yields suitable for diagnostic and high-throughput screening assays.

Methods of affinity separation wherein the affinity ligand is an immobilized proteinaceous ligand wherein one or more of its asparagine (Asn) residues has been modified. Methods of making a stabilized combinatorial protein by a) modification of Asn residues within a protein molecule to increase stability of the protein in alkaline conditions, and b) randomization of a protein molecule to modify its binding characteristics, and combinatorial proteins wherein in a step separate from the randomization step, the stability of the protein in alkaline conditions has been increased by modifying one or more of its Asn residues.

The present invention provides methods for characterizing a skin sample of a subject as belonging to an age range by analyzing nucleic acid or protein molecules obtained from the subject. The methods include analyzing expression or mutations in epidermal samples, of one or more skin markers. The methods can include the use of a microarray to analyze gene or protein profiles from a sample and compare them with a known skin age index. Therapeutic and cosmetic formulations are also provided herein.

The present invention provides the following: a method for efficiently producing a reagent for detecting an antibody that specifically binds with an insoluble antigen protein present in a liquid sample; a reagent for antibody detection produced by the production method; and a use of the antibody. In a step for solubilizing an antigen protein, it is possible to efficiently solubilize and recover the antigen protein by using a cationizing agent; therefore, when compared to conventional methods, it is possible to efficiently produce a reagent for detecting an antibody that has bound to multiple antigen protein molecules in a carrier.

The invention discloses a mussel mucoprotein liquid product as well as a preparation method and an application thereof. One or more of a modifier, an additive and a crosslinker are added into mussel mucoprotein to form a new product which can serve as a wound repair product, a wound protection product, a medical biological bonder product, a medical coating product, an industrial coating product, a biochemical reagent product or the like. According to the mussel mucoprotein liquid product and the preparation method of the mussel mucoprotein liquid product, the modifier, the additive and the crosslinker are added into the mussel mucoprotein, so that the stability of a protein structure is enhanced, the protein molecular weight is increased, and the mussel mucoprotein liquid product is widely applied in various fields.

The invention discloses a processing method for miscellaneous grain bean noodles. In specific to the problems that the miscellaneous grain beans contain no mucedin, and are difficult to form and have high broken rate when applied to noodle production, the method is used for developing nutritional and healthy noodles made from miscellaneous grain beans by means of the high and new food technologies such as a miscellaneous grain bean extrusion and pregelatinization processing technology, a multi-layer overlapping rolling technology of miscellaneous grain bean breads and wheat breads, an auxiliary preparation technology and the like. In the method disclosed by the invention, the protein molecules of the miscellaneous grain bean powder and the wheat powder are mutually combined to form a macro-molecular gluten network structure, thereby strengthening the toughness and a three-dimension space network structure of a dough are enhanced so that the strength and the extensibility of the dough are increased, and the whole dough has firmer network and better elasticity and toughness. In addition, on the basis of the technologies, the application of an ultra-micro powder physical modification technology can be used for effectively improving the quality indexes such as appearance, glossiness and the like of the noodles. By adoption of the invention, the adding amount of the miscellaneous grain beans in the noodles can be 10-90%.

The invention in suitable embodiments is directed to isolated bio-nanoparticle elements and isolated bio-nanoparticle platforms employing such isolated bio-nanoparticle elements. In one aspect, the isolated bio-nanoparticle elements are formed with purified Clathrin and or with purified coatomer I / II self-assembling protein molecules.

The invention discloses a silk fibroin micro-needle system, a silk fibroin nanometer particle and a preparation method thereof. The dissolvable silk fibroin micro-needle system comprises a base plate and a micro-needle, and is characterized in that a body of the micro-needle consists of silk fibroin protein condensate, the silk fibroin protein condensate penetrates into the skin of a user, and micro-pipes are remained on the skin of the user after the silk fibroin protein condensate is dissolved. The silk fibroin nanometer particle is characterized by comprising silk fibroin protein molecules and cosmetic molecules or medicine molecules, the silk fibroin protein molecules wrap the cosmetic molecules or the medicine molecules to form a particle structure, antibody or aptamer molecules can be attached onto the surface of the silk fibroin nanometer particle, and the degradation speed of the silk fibroin nanometer particle in the body of the user can be controlled by means of adjusting crystalline degree of silk fibroin protein. A method for delivering medicine or cosmetic is characterized by comprising steps of penetrating the silk fibroin protein condensate of the body of the micro-needle into the skin of the user, dissolving the silk fibroin protein condensate, and releasing nanometer silk fibroin particles wrapped with the medicine or the cosmetic so that the medicine molecules or the cosmetic molecules are delivered; or delivering the medicine molecules or the cosmetic molecules via the micro-pipes remained on the skin of the user after the silk fibroin protein condensate is dissolved in a permeation manner, and the like.

The invention relates generally to the field of plasmonics, and more specifically, in one embodiment, it relates to fabricating elements in whole or in part using one or more self-assembling elements comprised of purified, synthetic and or recombinant protein molecule elements and or their accessory elements, and in particular, composed of at least one or more Clathrin and or Coatomer I / II protein molecules, forming one or more self-assembling structure and framework elements of one or more molecular weights, dimensions, geometries, symmetries, configurations and combinations. In another aspect, the invention relates to a method using one or more nanoscale metal surface elements that, when one or more appropriate types or forms of energies are applied to one or more types of metal elements, emit one or more preferred types or forms of surface-plasmon-enhanced electromagnetic radiation and energy.

The present invention discloses a protein transistor device, wherein an antibody molecule (antibody-antigen) is bonded to at least two gold nanoparticles in a high reproducible self-assembly way to form molecular junctions, and wherein the two gold nanoparticles are respectively joined to a drain and a source. The protein transistor device can be controlled to regulate current via applying a bias to the gate. The conformational change of the protein molecule will cause the variation of the charge transport characteristics of the protein transistor device. The protein transistor device can be further controlled by different optical fields via conjugating a quantum dot to the molecular junctions. Therefore, the present invention has diversified applications.

The present invention provides a functionalized nanosubstrate or “nanotemplate” that is useful for selectively assembling nanoelements across a large area. The nanotemplate is capable of guiding the massive parallel assembly of nanoelements to fabricate a three-dimensional nanostructure. Nanoelements can also be transferred at a high-rate from the template to a recipient substrate. Examples of these nanoelements include, but are not limited to, carbon nanotubes, nanocrystals, dendrimers, nanoparticles, nanowires, biological materials, proteins, molecules and organic nanotubes. The invention also provides a nanotemplate combined with selectively assembled nanoelements. The invention encompasses methods for functionalizing a nanosubstrate. These methods involve providing a substrate having a known topology and polymerizing a monomer on its surface. Methods for selecting nanoelements and guiding their self-assembly are also disclosed. The invention further provides a method for modifying and transferring assembled nanoelements to a recipient substrate.

Methods and devices for the measurement of molecular binding interactions. Preferred embodiments provide real-time measurements of kinetic binding and disassociation of molecules including binding and disassociation of protein molecules with other protein molecules and with other molecules. In preferred embodiments ligands are immobilized within pores of a porous silicon interaction region produced in a silicon substrate, after which analytes suspended in a fluid are flowed over the porous silicon region. Binding reactions occur when analyte molecules diffuse closely enough to the ligands to become bound. Preferably the binding and subsequent disassociation reactions are observed utilizing a white light source and thin film interference techniques with spectrometers arranged to detect changes in indices of refraction in the region where the binding and disassociation reactions occur. In preferred embodiments both ligands and analytes are delivered by computer controlled robotic fluid flow control techniques to the porous silicon interaction regions through microfluidic flow channels.

The invention relates to a method for producing a protein molecule composition having a defined glycosylation pattern, comprising (a) introducing in a host cell which is an immortalized human blood cell at least one nucleic acid encoding at least a part of said protein; and (b) culturing said host cell under conditions which permit the production of said protein molecule composition; and (c) isolating said protein molecule composition.

The invention discloses a preparation method of a protein molecule engram film, comprising the following steps: 1. pretreatment of carrier sheet glass used for preparing a protein molecule engram film: putting a cover glass into a beaker, adding cleaning agent and deionized water, and processing by ultrasound; 2. preparation of the solution of template protein, functional monomer and crosslinking monomer: preparing template protein solution by phosphate buffer, adding water-solubility functional monomer, crosslinking monomer and initiator, and evenly mixing; 3. carrying out polymerization between sheet glasses to form a polymer film; 4. elution of template protein molecule: putting the molecule engram film into a beaker, adsorbing eluent in the beaker, removing the template protein molecule, and obtaining an engram hole matched with the template protein molecule. In the engram process of the invention, protein has small possibility of denaturation, and polymerization condition is simple and moderate. The prepared molecule engram film can be developed into a developing chip capable of detecting protein.

A precursor for the construction of chelated metal conjugates which demonstrate improved assay performance and utility in minimizing non-specific binding while maintaining specificity for target molecules is disclosed. The precursor has tridentate functionality towards multivalent ions such as iron and nickel and contains a diacetyl glycine group covalently linked via an amide to a molecule such as a proteinaceous molecule providing a primary amide group for amide bond formation. The precursor is preferably prepared in monomeric form by reacting nitrilotriacetic acid or a salt thereof in an aqueous medium at an alkaline pH of at least 8 with a proteinaceous molecule containing a primary amine group in the presence of a carbodiimide. The proteinaceous molecule may be bovine serum albumin or an enzyme such as alkaline phosphatase or horseradish peroxidase.

A method and apparatus is disclosed for mechanically-assisted liposuction treatment. The apparatus includes a hand-holdable housing, an electro-cauterizing cannula assembly, and a reciprocation mechanism. The hand-holdable housing has a cavity adaptable for receipt of a portion of the electro-cauterizing cannula assembly. The electro-cauterizing cannula assembly has a distal end and a proximal end and at least one aspiration aperture about the distal end. The reciprocation mechanism is disposed within the housing and is operably associated with either the cannula so that the cannulas can be selectively caused to reciprocate relative to the housing. As the cannula is caused to reciprocate relative to the housing, the aspiration aperture formed through the distal end of the cannula assembly is caused to undergo periodic displacement. During aspiration of tissue, high-voltage RF power signal supplied to an electro-cauterizing electrode structure formed about each reciprocating aspiration aperture to effect hemostasis thereabout. Such hemostasis is achieved by causing protein molecules within aspirated tissue to coagulate in response to the high-voltage RF signal being supplied across the electro-cauterizing electrode. In the preferred embodiments, the amount and rate of such aspiration aperture displacement is controllably adjustable. The cannula assembly is releasably detachable from the hand-holdable housing to facilitate cleaning and sterilization of the cannula assembly and the housing.

The invention relates to the field of cell and tissue culture, in particular to a culture medium for culturing mesenchymal stem cells. Aiming at the problems that the mesenchymal stem cells exist in an existing bovine serum culture medium and a human serum culture medium, the invention provides the culture medium for culturing the mesenchymal stem cells. According to the technical scheme, the culture medium for culturing the mesenchymal stem cells provided by the invention is characterized by comprising two kinds of components, namely a basal culture medium and culture medium additives; the additives comprise a cell growth factor combination, insulin, trace elements, lipid materials, vitamin substances, hormone-like substances, protein molecules, amino acid substances and other compounds. According to the culture medium for culturing the mesenchymal stem cells, provided by the invention, the zoonosis and the risk of human immune responses in the cell therapy process can be avoided, and the possibility that the natures of the cultured mesenchymal stem cells are different due to the difference among culture system batches can be avoided; the culture medium is suitable for culturing all the mesenchymal stem cells and is broad in prospect.

The present invention provides a protein micro-array surface plasma resonance imaging detection system and its detection method. Said system includes surface plasma resonance micro-array protein sensor, incident arm, reflecting arm and signal processing unit, the described sensor includes prism, micro-array chip and sample cell, between prism and micro-array chip a refractivity oil layer is coated, the incident arm is positioned at one side of the sensor, successively includes semiconductor laser, collimator, polarizer, attenuator and reactangular light diaphragm, the reflecting arm is positioned at another side of the sensor, and successively includes lens and CCD receiver, and signal processing unit includes signal processing circuit and computer.

The invention discloses a dyeing method of protein material. Arylamine compound is diazotized and then is directly coupled with tyrosine segment in protein molecule, so as to generate coloured substance containing azo bond; dosage of arylamine compound is equivalent to 0.1-5% of weight of protein material, and dyeing bath ratio is 1:20-100. Arylamine compound is taken as colour base and diazotized and then is directly coupled with tyrosine segment in protein molecule, and coloured substance connected with main chain by covalent bond in protein molecule, thus realizing dyeing on protein material and obtaining various colours by selecting arylamine colour bases in different structures. The dyeing method has the advantages of low energy consumption, less pollution and high colour fastness. The protein fiber fabric obtained by the method has higher wet-processing fastness; and the dyed protein powder also has higher fastness. The invention also provides a new dyeing method for protein material.

The invention discloses a silk hydroxyapatite composite stock and making method, which is characterized by the following: taking the hydroxyapatite and silk protein as base; taking the degelatinized cultivated silk or cloth as reinforcement; solidifying HA power in more homogeneous solution rapidly by adapting solution blending-freezing gel method; elevating temperature above the glass transition temperature of silk solution; adding low-molecular-weight effumable organic reagent to rearrange the silk protein molecule in the freezing solution; gelifing to fix HA powder; formatting pore of certain structure after detaching the water and organic reagent by heating; forming silk hydroxyapatite composite stock with multipore. The invention qualifies good dynamical property, cellular compatibility and right degradation speed to satisfy the need of cell cultivation bracket as bone injuring renovation, medicine control releasing and bone tissue engineering stock with broad prospect.

A method and apparatus is disclosed for mechanically-assisted liposuction treatment. The apparatus includes a hand-holdable housing, an electro-cauterizing dual cannula assembly, and a reciprocation mechanism. The hand-holdable housing has a cavity adaptable for receipt of the electro-cauterizing cannula assembly. The electro-cauterizing cannula assembly has a distal end and a proximal end and at least one aspiration aperture about the distal end. The reciprocation mechanism is disposed within the housing and is operably associated with the inner cannula so that the inner cannula can be selectively caused to reciprocate relative to the stationary outer cannula mounted to the hand-supportable housing. As the inner cannula is caused to reciprocate relative to the housing, the aspiration aperture formed through the distal end of the cannula assembly is caused to undergo periodic displacement. During aspiration of tissue, high-voltage RF power signal supplied to electro-cauterizing electrode structures formed about each reciprocating aspiration aperture to effect hemostasis thereabout. Such hemostasis is achieved by causing protein molecules within aspirated tissue to coagulate in response to the high-voltage RF signal being supplied across the electro-cauterizing electrode. In the preferred embodiments, the amount and rate of such aspiration aperture displacement is controllably adjustable. The cannula assembly is releasably detachable from the hand-holdable housing to facilitate cleaning and sterilization of the cannula assembly and the housing.

Compositions and methods are provided for the enhanced in vitro synthesis of protein molecules, by optimizing the metabolism of amino acids present in the reaction mix, preferably all amino acids in the reaction mixture. By performing synthesis with extracts from genetically modified microbial strains that are deficient in multiple amino acid metabolizing enzymes reduces the enzymatic activities responsible for catalyzing these reactions and improves the overall yield of synthesis.

The invention relates to the field of generating helper phages and phage display libraries for the identification of binding molecules. The invention provide chimaeric phages having a coat comprising a protein mixture. The protein mixture comprises a fusion protein having a proteinaceous molecule fused to a functional form of a phage coat protein and a mutant form of the phage coat protein, wherein the mutant form is impaired in binding to a host cell receptor. The invention further provides new phage collections, novel helper phages and methods and means for producing chimaeric phages, infectious phages and helper phages.