63 results about "Schizothorax" patented technology

An artificial reproduction method for schizothorax includes choosing parents fish, culturing, fertilization, incubation and culturing fry.

The invention provides a method for taming wild Lhasa Schizothorax. Parent fish is collected with an electric fishing method, the parent fish which is well-developed, vivacious and robust in health is selected as a taming object, potassium permanganate is used for disinfection, high protein dead bait and buoyancy pellet feed are selected and fed alternatively for taming, and the taming survival rate is higher. The taming method is simple, the operability is high, the piratical applicability is good, large-scale and standard taming of wild Lhasa Schizothorax can be realized, development of the Lhasa Schizothorax is promoted, and the wild wild Lhasa Schizothorax resource is effectively protected.

The invention discloses a schizothorax intensified and quick breeding method. Staged separated breeding is performed, and the aquatic water environment is continuously kept within a range appropriate for growth of schizothorax, so that ingestion and growth all the year round are realized; various kinds of fry bait are matched scientifically and reasonably to meet the growth requirements of schizothorax, so that the rate of survival in fry cultivation reaches about 90%, the breeding period from the fries to marketable fishes is shortened to about 2.5-3 years, the sizes of marketable fishes are consistent, and during the cultivation and breeding, no serious disease or loss is caused; besides, the method is simple to study, popularize and master, and has the advantage of high cultivation benefit (the input-output ratio is 1:5), and has huge application value and positive significance in promoting income increase and wealth acquisition at impoverished mountainous areas.

The invention discloses a network fishing detection method based on a douche group algorithm support vector machine. The method comprises the following steps of firstly, initializing basic parametersof the douche group algorithm, including population number, iteration times, individual dimension and search space; randomly initializing the position and range of the individual; and then dividing into leader vessel sea sheaths and follower vessel sea sheaths according to the magnitude of the fitness value, and excavating the optimal parameters of the support vector machine by using the coordination and cooperation of the two vessel sea sheaths. In each iteration, the function for evaluating the fitness value of the individual is the detection accuracy of the parameter carried by the individual on the phishing website data set by the support vector machine. Compared with the common optimization algorithms such as a genetic algorithm, a gravitation search algorithm, a bat algorithm and a particle swarm algorithm, the optimal parameter parameters of the support vector machine can be mined as much as possible on the optimized support vector machine, and the fishing detection accuracy ofthe support vector machine is improved.

The invention relates to a construction method for a anabarilius grahami cardiac cell line and belongs to the technical field of freshwater organism cell culture. The method includes using anabarilius grahami heart tissue as a material, washing and shearing the heart tissue, using hyaluronidase and II type collagenase to perform a joint digestion to obtain free heart cells and loose tissue blocks, inoculating the free heart cells and the loose tissue blocks in a culture flask of 25 square centimeters, adding an L-15 culture solution containing fetal calf serum, human basic fibroblast growth factor and chondroitin sulfate in the culture flask, and placing the culture flask in a culture box of 28 DEG C to culture. The culture solution is replaced at an interval of three and half days, and when cells grow into monolayers, a trypsin digestive method is utilized to perform cell passage. The construction method for the schizothorax taliensis cardiac cell line has the advantages that the operation is simple and convenient, the cell growth state of the constructed anabarilius grahami cardiac cell line is good, the cells are fibroblast-like, more than 40 generations can be continuously achieved during the cell passage, the constructed anabarilius grahami cardiac cell line can be directly used for models of an environmental toxicology for studying environmental pollutants to perform pollution detection and safety evaluation, and the method is also suitable for the construction of cardiac cell lines of other fishes.

The invention provides a muddy man-made spawning site selecting and building method of plateau river schizothorax. The method includes the steps: firstly, selecting a muddy man-made spawning site of the plateau river schizothorax; secondly, deeply digging the man-made spawning site to reach 30-40cm; thirdly, preparing mud substrates; fourthly, covering the man-made spawning site with the mud substrates; fifthly, remodeling the topography of the spawning site. According to the method, a suitable river section is selected, the flow velocity of the area is low in flooding, so that the area adoptto spawning of the schizothorax, eggs cannot be carried away by water flow, the topography of the spawning site is flat, so that the eggs are easily attached into spawning pits, bottom substrates of the spawning site are created, pit digging behaviors of the schizothorax can be met, stability of the spawning site and the spawning pits can be maintained, and the spawning pits cannot collapse afterthe schizothorax spawns.

The invention discloses a micro-flowing-water ecological breeding method for rare fishes in a rice field. The breeding method comprises the following steps of (1) selection of the rice field; (2) rice field transformation, wherein an enclosing ridge is built around the rice field, a water inlet and a water outlet are formed in the enclosing ridge of the two opposite corners of the rice field, then, fish ditches of cross-shaped structures are dug in the rice field, and finally, a feeding breeding ditch communicated with the fish ditches is dug in one side of the rice field; (3) rice planting, wherein manual transplanting of rice seedlings is conducted in rice planting areas in the rice field; (4) fry release, wherein 15 days after transplanting of the rice seedlings, fry are stocked in the feeding breeding ditch, and most of fry of sinocyclocheilus graham, schizothorax taliensis, neolissochilus benasi and spinibarbus sinensis bleeker are stocked; (5) feeding management, wherein after the planted rice seedlings turn green, artificial feed needs to be added into the feeding breeding ditch every day; (6) daily management. The allocation of mixedly-bred fry types is reasonable, planning for the rice field is proper, a scientific feeding mode is adopted, the growth speed of the fry is increased, and the yield of rice is also increased, so that the benefit of mixed fish breeding in the rice field is significantly increased.

The invention discloses a slope type schizothorax taliensis artificial propagation spawning device and belongs to the technical field of schizothorax taliensis artificial propagation. The device comprises a spawning bed and a water diversion pipe. The spawning bed comprises impact slopes, side baffles, water baffles, rest platforms and supports. The rest platforms are sequentially arranged in a stepped mode from top to bottom. The rest platforms are connected through the inclined impact slopes. The side baffles are arranged on the two sides of the rest platforms and the two sides of the impact slopes. The water baffles are arranged at the front ends of the rest platforms. The rest platforms are sequentially placed on the supports from bottom to top. The spawning bed is provided with obstacles capable of rubbing with schizothorax taliensis to stimulate the schizothorax taliensis to spawn. The water diversion pipe is arranged above the uppermost rest platform. According to the device, conditions suitable for schizothorax taliensis spawning are created in a fish pond, the schizothorax taliensis is effectively stimulated to spawn and discharge seminal fluid, and meanwhile the sperm and egg binding efficiency is improved.

An oxygenation-type schizothorax breeding tank comprises a tank body. An opening is formed in the top side of the tank body, an inner tank is arranged in the middle of the upper portion of the tank body, a second one-way valve is fixedly mounted in each air outlet, and the inner ends of the top sides of inverted-L-shaped boxes on two sides are fixedly connected through a second filter net. The oxygenation-type schizothorax breeding tank is simple in structure and ingenious in concept, pressure generated by impact force of water to the tank wall when an automobile travels on a bumpy road is utilized to drive a piston to move to press air into the tank body to realize oxygenation, and a spring enables the piston to be reset, so that constant oxygenation on the bumpy road can be realized; theinner tank can swing along with impact of the water to the inner wall of the tank body, and feed at the bottom of the inner tank can be thrown up, so that convenience is brought to fishes to eat thefeed sinking to the bottom, utilization rate of the feed is increased, and pollution of water by the feed is reduced; fish dung can be collected, so that water quality is further improved, market needs can be met, and the oxygenation-type schizothorax breeding tank is suitable for popularization.

The invention provides an instant compounded-taste Schizothorax fish soup and a preparation method thereof. The method comprises the following steps of: (1) soaking fresh Schizothorax fish with 1wt% yeast powder solution for 1-3 hours, then pan-frying the soaked fresh Schizothorax fish in hot oil until the Schizothorax fish becomes light yellow; (2) boiling the substance obtained in the step (1) in water until the water is boiled, further boiling with low fire for 0.5-1.5 hours, then adding vegetable granules at a vegetable granule and Schizothorax fish mass ratio of 1 to (9-11), further boiling out with low fire for 2-3 hours, and adding a proper amount of salt and monosodium glutamate, wherein the vegetable granules are lentinus edodes granules or endive sprout granules; and (3) filtering the substance obtained in the step (2), reserving cooking liquor for later use, removing bony spurs in filter residues, then grinding the filter residues, boiling out the ground filter residues in water, and filtering the boiled filter residues to obtain cooking liquor; and (4) mixing the obtained cooking liquors, and performing vacuum concentration on the obtained cooking liquor, thereby obtaining the instant compounded-taste Schizothorax fish soup. The preparation method is simple; the instant compounded-taste Schizothorax fish soup is easy to carry, not only reserves the unique flavor of Schizothorax, but also has the fragrance of endive sprout and lentinus edodes.

The invention provides a breeding system of schizothorax seedlings in the Tibet plateau. The system includes a fish pond, wherein a water outlet of the fish pond is sequentially connected with a watertreatment system and a dissolved oxygen device through pipelines, the dissolved oxygen device is connected with a water inlet of the fish pond through a pipeline, the water outlet is opposite to thewater inlet, a bottom surface of the fish pond is a slope of which one end of a water inlet is higher than one end of a water outlet, the dissolved oxygen device includes a casing and a liquid inlet chamber, an air inlet chamber, a diffusion chamber and a mixing chamber disposed in the casing, and the liquid inlet chamber is composed of a liquid inlet channel and a first injection chamber communicated with the liquid inlet channel. The system is advantaged in that the system is especially suitable for the high altitude environment in Tibet with high altitude, low dissolved oxygen and large temperature difference between day and night.

An artificial propagation and spawning device for Schizothorax spp. comprises a support plate; a plurality of vertical sliding grooves are formed in the top surface of the support plate, and sliding blocks are respectively arranged in the sliding grooves; the top surfaces of the sliding blocks are respectively hinged to the bottom ends of rocking rods, and the bottom surfaces of the sliding blocksare respectively connected with the inner walls of the bottom surfaces of the corresponding sliding grooves through springs; a hatching box with an open top surface is arranged above the support plate, and the top ends of the rocking rods are hinged to four corners of the bottom surface of the hatching box respectively; the top surface of a sector gear is fixedly mounted in the middle of the bottom surface of the hatching box; the top surface of the support plate is provided with a motor through a bracket, and the outer periphery of an output shaft of the motor is fixedly fitted with the inner circle of a gear; and the gear is meshed with the sector gear. Dead eggs are regularly removed through the hatching box and a dead egg removing device, the dead eggs in the hatching box can be basically discharged through periodic operation of the dead egg removing device, the pollution of the dead eggs to a hatching liquid is avoided, the purity of the hatching liquid is guaranteed, the hatching condition of manual propagation is further guaranteed, and manually clearing the dead eggs is not required so that the labor cost is saved.

The invention relates to a schizothorax taliensis breeding and culturing method, which comprises the following steps of (1) artificially domesticating collected wild high mountain schizothorax taliensis for 3 to 4 years; (2) from the middle of January to the late March of each year, selecting mature female fishes and male fishes in the step (1), mixing eggs extruded from the female fishes and seminal fluid taken out from the male fishes, placing in clean water, keeping for 10 to 20min in a dark place, and thus obtaining germ cells; (3) placing the germ cells into flowing clean water, incubating for germinating, and starting to feed baits for fish larvae after germinating for 7 to 9 days. The method provided by the invention is scientific and reasonable, and high in fish egg survival rate,the fish egg fertility rate reaches up to 95 percent or more, the fish egg hatching rate reaches up to 85 percent or more, and the fish larvae survival rate reaches up to 90 percent or more.

The invention discloses a method for identifying the sexual maturity of parent fish of schizothorax. The method is characterized by comprising the following steps of fishing healthy male and female parent fish reaching sexual maturity from a parent fish culture pond in a mating season of the schizothorax; scooping up the fish in a pre-prepared container or a fish clamp; watching the rostral side of the head part of the fish through naked eyes, and determining that fish with bead stars is male fish and the fish without the bead stars is female fish. By adopting the method for checking the caudal peduncle of the male and female parent fish by adopting a magnifying lens, the method has the advantages that distinguishing on fish sexual maturity is accurate and fast, the fish is not damaged, the operation is convenient, and the method can be easily mastered.

The invention provides an artificial hatching culture pond for Schizothorax taliensis. The artificial hatching culture pond comprises a hatching pond and a disinfectant pool, wherein the disinfectant pool comprises a sterilizing pool, a biochemical blanket pool, a biochemical ball pool and a filtering brush pool which are sequentially arranged and mutually communicated; the sterilizing pool is communicated with the hatching pond and is used for conveying purified clean water into the hatching pond; the filtering brush pool is communicated with the hatching pond and is used for performing purification treatment on sewage in the hatching pond; a net box is arranged in the hatching pond; an artificial fish nest is placed in the net box; an aerator is arranged at the bottom of the hatching pond; a temperature sensors is arranged on the pool wall of the hatching pond. The hatching pond provides a hatching environment with good water quality, abundant dissolved oxygen, proper temperature and proper water speed for hatching fish roes; in addition, high-quality water can be effectively and circularly provided for the hatching pond.

Disclosed is an auxiliary breeding device for schizothorax prenati. The auxiliary breeding device for schizothorax prenati comprises a pond with an opening in the top surface, a box body with an opening in the top surface is fixedly installed at the right side of the pond, and the pond is internally provided with a plurality of U-shaped pipes; a through groove is communicated with the interior ofthe box body, the right end of a filtering plate is clamped in the through groove, the right portion of the through groove is provided with a cover plate, and a plurality of evenly-distributed baffleplates are fixedly installed at the outer periphery of a rotating shaft; the other end of a connecting pipe is always located above one of the baffle plates, the water level in the pond is always higher than that in the box body, the left side of the water pond is provided with a water temperature sensor, and the water temperature sensor is fixedly installed on the inner wall of the rear side of the pond. The auxiliary breeding device for schizothorax prenati is simple in structure, convenient to use and operate, beneficial to cleaning the filtering plate and capable of bringing use convenience; the water temperature sensor can detect the water temperature in the pond, so that a user conveniently knows the water temperature in the pond and regulates the water temperature in the pond accordingly, and it is always ensured that the water temperature in the pond is within the range of temperature suitable for living of schizothorax prenati.

An aeration device for schizothorax fish culture comprises an annular plate. The bottom side of a box is fixedly connected to the middle of the top side of the annular plate, and a vertical tube is fixedly mounted in the annular plate. The upper end of a tapered tube is fixedly connected with a lower port of a three-way tube. An upper port of the three-way tube is fixedly connected with a water inlet of a first electromagnetic valve, and an annular floating plate is fixedly mounted on the bottom side of the annular plate (the buoyancy of the annular floating plate is large enough to carry theannular plate and devices above the annular plate, and the diameter of the annular floating plate is large enough to avoid the annular plate from tilting to one side). The aeration device has the advantages that the aeration device has a simple structure and ingenious design and integrates water spray aeration with inflation aeration, aeration at different depths of a fish pond can be realized, aeration flows can be diffused in a wide range, disturbance of inflation aeration on fish manure at the bottom of the fish pond can be reduced, the fish manure at the bottom of the fish pond can be collected by water spray aeration, a large amount of the fish manure accumulated under an aerator can be cleaned to improve the fish pond, market demands can be met, and the aeration device is suitable for promotion.

The invention relates to a pond cultivation method for wild schizothorax. The pond cultivation method comprises the following specific steps of (1) pond building; (2) pond water selecting; (3) fry selecting; (4) breeding in a pond; (5) middle term management; and (6) harvesting. By means of the pond cultivation method for the wild schizothorax, feeding of the wild schizothorax in the pond can be achieved effectively, the wild physique of the wild schizothorax is guaranteed, meanwhile, the growing speed of the schizothorax is increased, the growing cycle of the schizothorax is shortened, and the economic profit of the schizothorax is increased effectively.

The invention provides a feed capable of promoting propagation capacity of schizothorax taliensis. The feed comprises the following raw materials in parts by weight of 55-65 parts of fish meal, 30-40parts of castor seed cakes, 8-16 parts of shrimp powder, 10-20 parts of wheat flour, 20-30 parts of soybean meal, 16-25 parts of corn protein powder, 15-26 parts of peanut meal, 1-3parts of a feed additive, 1.5-2.5 parts of soybean phospholipid and 0.9-1.8 parts of a multi-vitamin premix, wherein the feed additive comprises the following raw materials in parts by weight of 16-24 parts of earthwormextracts and ferrous fumarate, 12-18 parts of coix seeds, 6-12 parts of black rice, 5-10 parts of kudzu vine flowers, 4-8 parts of radix astragali, 1-2 parts of turtle shells, 0.8-1.6 parts of unsaturated fatty acid and 15-20 parts of carriers, the earthworm extracts and the ferrous fumarate need to be subjected to magnetisation treatment in advance, and the mass ratio of the earthworm extracts to the ferrous fumarate is (60-70) to 1. The feed can increase the propagation capacity, the hatching rate and the larva fish survival rate of the schizothorax taliensis.

The invention relates to fish feed with a function of improving the aquaculture efficiency, a method for preparing the fish feed and a method for feeding schizothorax prenati with the fish feed. The fish feed with the function of improving the aquaculture efficiency comprises, by weight, 35-45 parts of fish meal, 16-24 parts of bean pulp, 10-12 parts of starch, 1-4 parts of rapeseed oil, 0.8-1.5 parts of calcium dihydrogen phosphate, 1-2 parts of minerals, 0.8-1.2 parts of choline, 80-100 parts of water and 1-3 parts of konjac glucomannan. The fish feed and the methods have the advantages thatthe konjac glucomannan is added into the fish feed, the immune performance of the schizothorax prenati can be obviously enhanced after the schizothorax prenati is fed with the fish feed, the survivalrate can be obviously increased, the economic benefit can be effectively increased, and the aquaculture cost can be reduced.

A schizothorax quadricarinatus fin ray cell culture bioreactor comprises a base, vertical supporting plates are fixedly installed on the two sides of the base respectively, a motor is fixedly installed in the center of the base, a sliding block with a dovetail-shaped section is arranged in a sliding groove in a matched mode, the bottom end of the sliding block is hinged to the top end of the otherside of a connecting plate, and an air inlet is formed in one side of a sleeve. A pipeline is fixedly connected to the top end of the sleeve and communicated with the interior of the sleeve, and theother end of the pipeline is fixedly connected with the top end of the reactor body through a sealing bearing and communicated with the interior of the reactor body. According to the present invention, the shaking rotation can be performed, the shearing force generated by the machine is reduced, the influence of the mechanical stirring on the cells is avoided, the damage or the infection of the cells caused by the mechanical stirring is avoided, other devices are not required to provide power for the oxygen supply, the oxygen supply is performed through the movement of the reactor body, and the power device is less; better effect.

The invention provides feed for juvenile schizothorax taliensis. The feed comprises, by weight, 20-30 parts of wheat bran, 30-40 parts of fish meal, 5-10 parts of plant oil, 5-8 parts of lotus root starch, 3-5 parts of yam flour, 5-8 parts of glucose, 20-30 parts of bean pulp, 10-15 parts of raw eggs, 10-12 parts of earthworm powder, 5-8 parts of soybean lecithin, 8-10 parts of fish oil, 5-10 parts of inkfish grease, 0.3-0.5 part of monocalcium phosphate, 0.1-0.2 part of phagostimulant, 10-15 parts of strong flour, 1-2 parts of mineral additives, 0.5-0.8 parts of vitamin additives, 0.3-0.8 part of vitamin C acetate, 0.03-0.05 part of high-temperature-resisting phytase, 0.03-0.08 part of hymecromone, 8-12 parts of dried pig blood and 0.1-0.3 part of yeast extract. The feed for the juvenileschizothorax taliensis has the advantages that the content of protein and grease is high, nutrient substances are balanced, the juvenile schizothorax taliensis absorbs and digests the nutrient substances conveniently, growth speed of the schizothorax taliensis is increased, feeding of the schizothorax taliensis is promoted, and palatability of the feed is improved.

The present invention discloses a detection device for schizothorax fish cell variability. The detection device for the schizothorax fish cell variability comprises a box body, a cell culture container is arranged in the box body, a horizontal liquid outlet is formed in a bottom part of a right side of the cell culture container, an outer end of the liquid outlet is connected with a left end of aninner threaded pipe through a sealing bearing, a right end of the inner threaded pipe is connected with a left end of a straight pipe through a sealing bearing, microscopes are arranged at lens endsof high-definition cameras in series, and a plurality of rubber supporting bases distributed at equal intervals are fixedly arranged on a top face of a conveying belt. A plurality of groups of conicaltubes, droppers, glass slides and conveying rollers are adopted, schizothorax fish cells in a schizothorax fish cell culture solution in the same period are sequentially subjected to multiple groupsof observation and detection, the whole detection accuracy is improved by using a multi-group parallel experiment, at the same time, the detection device uses an automatic operation, researchers onlyneed to compare and analyze schizothorax fish cell conditions in the schizothorax fish cell culture solution shot or recorded by the high-definition cameras, workload of the researchers is effectivelyreduced, and the time and the labor are saved.