19 results about "Schizothorax" patented technology

The invention relates to the technical field of fishery breeding, and particularly discloses a fry hatching device for schizothorax breeding, comprising a support frame, the top of the support frame is fixedly connected with a hatching box and a breeding box, the side surface of the hatching box is fixedly connected with the side surface of the breeding box, and two sides of the inner wall of the breeding box are fixedly connected with plastic plates; the fry hatching equipment comprises a hatching box and a breeding box, the side faces of the hatching box and the breeding box communicate with water adding pipes, the bottom of the side face of the hatching box penetrates through and is fixedly connected with a purification assembly, and an inlet is formed in the side, close to the hatching box, of the inner wall of the breeding box. And the aeration device is arranged, so that the content of dissolved oxygen in the incubator can be increased, sufficient oxygen is provided for fertilized eggs, and healthy growth of the fertilized eggs is promoted.

The invention discloses a cross breeding method for anti-ichthyophthirius mongholicus schizothorax prenanti. The method comprises the following steps: 1) selecting parents; (2) carrying out intensive cultivation on the parents; 3) synchronous artificial spawning induction; 4) artificial insemination; 5) hatching management; (6) breeding the schizothorax prenanti (female) and percocypris pingi (sex) hybrid fry; and (7) measuring the ichthyophthirius multifiliis resistance and high-temperature tolerance of the schizothorax prenanti (female) * percocypris pingi (sex) hybrid fry. The ichthyophthirius ichthyophthirius infection rate of the bred schizothorax prenanti (female) and percocypris pingi (sex) hybrid offspring is reduced by 31.95% compared with that of the schizothorax prenanti (female) and percocypris pingi (sex) selfing offspring; the death rate is only 0.33% and is reduced by 73% compared with that of the selfing filial generation of the schizothorax prenanti; the high-temperature tolerance range of the schizothorax prenanti selfing offspring is improved by 4-5 DEG C compared with that of the schizothorax Therefore, the method disclosed by the invention can be used for successfully culturing a hybrid variety which is high in ichthyophthirius multifiliis resistance, relatively high in economic value and relatively high in high-temperature tolerance.

This invention discloses an artificial breeding method for *Schizothorax lancensis*, belonging to the field of aquatic animal breeding technology. The artificial breeding method for *Schizothorax lancensis* provided by this invention includes: intensive three-step cultivation of broodstock, injection of spawning induced agents after cultivation, artificial fertilization, hatching of fertilized eggs, and fry cultivation. Using the artificial breeding method of this invention, the success rate of spawning induction in female *Schizothorax lancensis* is 83.3%, the average fertilization rate is 87.5%, the average hatching rate is 93.0%, and the average fry emergence rate is 91.5%. This achieves large-scale artificial breeding of *Schizothorax lancensis*, laying the foundation for preventing the extinction of *Schizothorax lancensis*, and for carrying out the propagation and release of *Schizothorax lancensis* for industrial utilization.

The present application relates to the technical field of soft-spined naked schizothorax breeding, in particular to a water quality and temperature detection device for soft-spined naked schizothorax breeding, which comprises a multifunctional sensor, a depth adjusting member, a protective cover member and an automatic cleaning member. The depth adjusting member is provided with the multifunctional sensor at the lower end. The protective cover member is rotatably connected with the depth adjusting member. The automatic cleaning member is slidably connected with the multifunctional sensor. The depth adjusting member comprises a ring-shaped air bag and a shell. The ring-shaped air bag is fixedly connected with the shell. The device can clean the dirt on the multifunctional sensor in time, realize accurate measurement, measure the temperature and water quality of the whole water area for full water layer profile measurement, timely regulate the local area temperature and find the water quality problem, prevent the multifunctional sensor from being impacted when sinking, ensure the direct contact of the multifunctional sensor with the water area environment, and realize accurate real-time measurement of water quality and water temperature.

The invention discloses an artificial compound feed for improving collagen deposition of schizothorax grahami and application of the artificial compound feed. The feed additive is characterized in that proline, hydroxyproline, eucommia ulmoides, broad beans and a yeast extract are added, and collagen deposition of the schizothorax grahami is improved under the condition that healthy growth of the schizothorax grahami is not affected. The feed comprises fish meal, fermented soybean meal, soybean protein concentrate, corn protein powder, fish oil, soybean oil, soybean lecithin, alpha-starch, cassava starch, choline chloride, microcrystalline cellulose, compound vitamins, compound minerals, monocalcium phosphate, proline, hydroxyproline, eucommia ulmoides, broad beans, a yeast extract, an antioxidant and a mildew preventive. The feed comprises the following nutritional ingredients in percentage by weight: 40.825%-41.407% of crude protein, 8.132%-8.633% of crude fat, and the balance of carbohydrate, crude fiber, ash and water. According to the artificial compound feed disclosed by the invention, the collagen deposition of the schizothorax grahami is remarkably improved, and the artificial compound feed also has a certain promoting effect on the protein deposition of the schizothorax grahami.

The invention relates to the technical field of schizothorax taliensis culture, in particular to a schizothorax taliensis culture water treatment and purification device which comprises a culture pond, a purification component, a centrifugal stirring component, an oxygen explosion component, a sedimentation pond, a flow guide plate, an oxygen exposure pond, a biological purification pond and a drainage part, the culture pond is connected with the purification component, and the sedimentation pond is provided with the centrifugal stirring component and the flow guide plate; the sedimentation tank is provided with an oxygen explosion component, and the drainage part is connected with the oxygen aeration tank, the biological purification tank and the culture pond. Impurities are conveyed through the purification component, dirt in a pipe is stripped off, blockage is prevented, sedimentation is accelerated through the centrifugal stirring component, aeration is conducted through the oxygen explosion component, and then water is sucked into the biological purification tank through the drainage part to be sterilized; the purification efficiency is improved, the natural water environment is simulated, interference to fish schools is small, and blockage is not prone to occurring.

The invention provides a device and a method for selecting substrates with suitable grain sizes for schizothorax fries. The device comprises a cylindrical water tank, a separation assembly and a life support system. The water tank is divided into a central larva fish throwing area and a plurality of fan-shaped substrate areas by partition assemblies, and gravel substrates with the same gradient particle size (1-30 cm) are laid in the symmetrical areas; the life support system maintains the water temperature to be 16-18 DEG C, the dissolved oxygen to be 6-7 mg / L and the water flow to be stable. The method comprises the steps that a substrate is laid, environment parameters are adjusted, 100-200 healthy larva fishes are put in, after adaptation, a baffle is pulled away so that the substrate can be freely selected, after one hour, the baffle is put back for partition counting, and the appropriate particle size is determined through a preference degree formula (P = (n / N) * 100%). Free selection and accurate counting are achieved through the detachable baffle, the problem of observation deviation of a traditional device is solved, and the result provides reference for sediment construction of a schizothorax spawning site.

The invention provides a method for constructing an artificial breeding schizothorax wangchizoides intestinal flora structure to digest field algae food, which comprises the following steps: A, collecting healthy adult schizothorax wangchizoides from the field as a donor, and preparing the intestinal content of the adult schizothorax wangchizoides into a coprophilous fungus suspension; selecting artificially fed healthy schizothorax brachypodum juvenile fish as a receptor; b, within 10-20 days, performing coprophilous fungus transplantation on the juvenile schizothorax wangchizoides in an overnight soaking manner every 3 days, and randomly sampling and selecting the schizothorax wangchizoides every 5 days in the afternoon for intestinal sample collection; and C, evaluating the intestinal flora in the sample, and if the receptor intestinal cellulose bacteria are successfully colonized, releasing to a natural river.

This utility model discloses a short-barbel schizothorax breeding and release chamber, relating to the field of fish fry farming technology. It includes a box body, inside which a chamber is installed. The inner wall of the chamber is equipped with a filter frame for filtering feed, fish feces, and uneaten feed. A temperature sensor is installed on one side of the box body for monitoring the temperature inside the chamber. A lid is rotatably connected to the top of the box body via a hinge. A shell is fixed to the inner wall of the lid, and a feeding assembly is installed inside the shell. The beneficial effects of this utility model are: the feed is guided to different outlets by a flow guide frame; the feed enters the chamber through the outlets and the feeding port on the lid, achieving feed addition to different locations inside the chamber, improving feeding uniformity; and the filter frame filters out pollutants, preventing their accumulation in the chamber, maintaining water quality, and providing good living conditions for fish fry.

The invention provides a schizothorax fish fertilized egg ultraviolet radiation damage experiment device and method. The device comprises a life support system, a main experiment system, a water inlet and outlet system and an oxygen supply system. The top of the main experimental system is divided into four independent areas, a metal net bag is hung in each area through a fixing device, stable water flow is formed at the 45-degree inclination angle of the tail end of a water inlet pipe, and the life support system maintains the water temperature to be 13-18 DEG C and the water quality to be stable. The method comprises the following steps: acquiring ultraviolet data in a natural reproduction period, setting intensity (low / medium / high) and time (short / medium / long) gradients, irradiating fertilized eggs from P1 to P7 periods, counting a death rate and analyzing a semi-lethal dose. Water environment interference is eliminated through integrated design, an accurate and efficient ultraviolet radiation injury experiment is achieved, and the device is suitable for schizothorax fish fertilized egg protection research. Water environment interference is eliminated through integrated design, an accurate and efficient ultraviolet radiation injury experiment is achieved, and the device is suitable for schizothorax fish fertilized egg protection research.

The invention relates to the technical field of molecular detection, in particular to a primer group, a probe and a kit for detecting schizothorax in a water environment. According to the primer group for detecting the schizothorax in the water environment, provided by the invention, the nucleotide sequence of the primer group is as shown in SEQ ID NO: 1 to SEQ ID NO: 2, or as shown in SEQ ID NO: 3 to SEQ ID NO: 4. The primer group for detecting the schizothorax has high specificity on the schizothorax, target gene segments of the schizothorax can be stably and effectively amplified, meanwhile, the specific primer group is used for conducting distribution investigation on the schizothorax in a water area on a water body sample, and the primer group can be used for detecting the low-concentration schizothorax and has good application prospects. The concentration difference between different sampling points can be effectively reflected, and a foundation is laid for exploring spawning sites and habitats of the schizothorax, realizing habitat restoration of the schizothorax and protecting species of the schizothorax.

The Lancang schizothorax fry rearing device is characterized in that a water inlet channel, a water outlet channel and a circulating pump are connected to the front side wall and the rear side wall of a parent fish culture pond respectively, and a filtering assembly is further arranged in the water inlet channel; an outer-layer pore plate and an inner-layer pore plate which are mounted in a relatively sliding manner are arranged at the communicated parts of the two channels and the parent fish pond, and water passing holes which are uniformly distributed are formed in each pore plate; a sliding partition plate and a sliding filter plate are arranged at the position, close to the bottom, in the parent fish pond, and a flashboard is vertically installed at a slag discharging hole in the pond wall of the parent fish pond in a sliding mode. The driving assembly enables the sliding partition plate and the sliding filter plate to slide reversely in sequence, so that during cleaning, the driving assembly drives the sliding partition plate to be inserted into the parent fish pond firstly to separate water above the parent fish pond, after the sliding partition plate is inserted in place, the flashboard slides upwards to thoroughly open the slag discharging hole, and the sliding filter plate slides out towards the outer side of the parent fish pond. The Lancang schizothorax can be bred under dynamic water flow, gonad maturation is promoted, and the parent fish pond can be conveniently and frequently cleaned without fishing.

This invention relates to the field of fish fry breeding and aquaculture technology, and specifically discloses a feeding device for breeding and aquaculture of Schizothorax bream fry, comprising: a frame, with support rods fixedly connected to the outer side of the frame, a buoyancy ball fixedly connected to the end of the support rods away from the frame via a bracket, a feed storage box fixedly connected to the top of the frame, a feeding port on the top of the feed storage box, and a discharge frame connected to the bottom of the feed storage box; a feeding device, the top of which is fixedly connected to the outer side of the frame; and a diffusion device, the outer side of which is fixedly connected to the outer side of the frame via a bracket; multiple sets of support rods are provided, evenly distributed on the outer side of the frame; wherein, the feeding device includes a feeding roller. This feeding device for breeding and aquaculture of Schizothorax bream fry can distribute a fixed amount of feed into the breeding pond and can adjust the feeding amount.

An experimental method for low-high temperature hatching stimulation and dual-high temperature tolerance threshold determination for schizothoracine fish eggs in high diurnal temperature variation waters includes: step 1, determining daily extreme water temperatures and a daily water temperature variation during natural incubation of the schizothoracine fish eggs; step 2, implementing an incubation water temperature with the daily water temperature variation; step 3, determining a key hatching water temperature; step 4, inducing mass hatching of the schizothoracine embryos; and step 5, determining temperature tolerance thresholds. Through indoor water temperature gradient experiments on the schizothoracine fish eggs, a hatching mechanism under low-high temperature stimulation for fertilized eggs of Schizopygopsis microcephalus in Yangtze River source, and dual-high temperature tolerance ranges and thresholds. This provides scientific support for the protection and restoration of fishery resources in the Yangtze River source under the background of global warming.

The invention relates to a breeding method and application of schizothorax contribution and belongs to the technical field of aquaculture. According to the method, large-scale artificial breeding of the wild schizothorax tribute is achieved through the steps of parent fish capturing, parent fish temporary rearing, artificial spawning induction, artificial insemination, hatching, fry rearing and the like, the spawning induction rate, the fertilization rate, the hatching rate and the emergence rate are high, the whole artificial breeding process can be guaranteed, and the survival rate of the wild schizothorax tribute is increased. Therefore, technical support and theoretical basis are provided for germplasm resource protection, development and utilization of schizothorax tribute.

The invention relates to the technical field of biology, in particular to a primer composition for detecting MNP marker sites of schizothorax prenanti. According to the primer composition for detecting the MNP marker sites of the schizothorax prenanti, the nucleotide sequences of the primer composition for detecting the MNP marker sites of the schizothorax prenanti are as shown in SEQ ID NO: 1 to SEQ ID NO: 230. The distinguishing degree of MNP marker sites of a sample to be detected is analyzed on the basis of extracted sample re-sequencing data, 115 MNP marker sites of the schizothorax prenanti are obtained, and 115 pairs of primers are designed on the basis of the obtained 115 MNP marker sites to form the primer composition for identifying the schizothorax prenanti.

The invention relates to the technical field of aquaculture, in particular to a schizothorax filiformis breeding device which comprises a breeding box, a water supply pipe roe collecting component, a protection component, a tail water treatment component, a sewage draining exit, a sewage draining pond and an intercepting net, the breeding box is fixedly connected with the water supply pipe, the sewage draining exit is formed in the bottom of the breeding box, the sewage draining pond is arranged at the bottom of the breeding box, and the intercepting net is fixedly connected with the water supply pipe. When the schizothorax filiformis is cultured, in order to assist reproduction of the schizothorax filiformis, roes are collected through the roe collecting component, culture personnel lift and pull the roe collecting component, then roe collection can be completed, convenience and rapidness are achieved, and when pollution discharge and water change are needed in culture, the fish roes can be collected through the tail water treatment component. Meanwhile, the tail water treatment component drives the protection component to be closed, so that roe loss in the tail water discharge process is prevented, it is ensured that roes are not damaged by tail water discharge, and meanwhile the water quality is efficiently treated.

The present application relates to the field of aquaculture technology, and more particularly to a long filament schizothorax artificial breeding method, which comprises the following steps: (1) temporarily raising the captured wild long filament schizothorax in an ecological simulation pool, and then transferring the long filament schizothorax into a special breeding pool for intensive cultivation; (2) selecting parent fish and injecting oxytocin; (3) artificially inseminating the female fish about to give birth, and then putting the parent fish into a postpartum cultivation pool for postpartum cultivation, hatching long filament schizothorax fertilized eggs, and obtaining long filament schizothorax fry; and (4) primary cultivation of the long filament schizothorax fry until the body length is greater than 2 cm. The present application includes the processes of oxytocin injection, spawning, insemination, hatching and cultivation of fry of long filament schizothorax, has a high hatching rate of fertilized eggs, and fast growth rate of fry, and provides a technical basis for artificial breeding of long filament schizothorax.

The invention provides an identification and analysis method for breeding biological characteristics of schizothorax in a plateau area. The invention discloses a plateau region schizothorax fish breeding biological characteristic identification and analysis method. The method comprises the following steps: S1, sample collection and basic data acquisition; s2, fish gonad development staging and reproduction cycle determination; s3, accurate age identification and growth relation establishment; s4, primary sexual maturity identification and analysis; s5, determining breeding seasons and periods; s6, determining spawning types; s7, high-precision fish fecundity determination and analysis; s8, carrying out correlation analysis on the reproductive capacity model and the environmental factors; and S9, evaluating a population breeding strategy and vulnerability. The plateau region schizothorax breeding biological characteristic identification and analysis method provided by the invention has the advantages that complete process operation from sample treatment to ecological evaluation can be realized, and the problems of scattered methods, low data precision and disjunction with environmental factors in the prior art can be solved.