The invention provides a combined detection kit for human tumor gene mutation. The combined detection kit comprises the following parts: an EGFR (Epidermal Growth Factor Receptor) gene primer group, a KRAS gene primer group, a BRAF (serine-threonine kinase) gene primer group, an NRAS gene primer group, a PIK3CA (Phosphatidylino-sitol 3-kinases) gene primer group, a HiFi enzyme, a PCR (Polymerase Chain Reaction) reaction solution, a digestive enzyme, ligase, a ligation buffering solution, a ligation joint, a fluorescent probe, a Taq enzyme of QPCR (Quantitative Polymerase Chain Reaction) reaction, a QPCR primer and a QPCR reaction solution. The combined detection kit provided by the invention can be used for simultaneously detecting mutant sites of a plurality of driving genes of human tumors, including one or more gene mutant sites of EGFR, NRAS, KRAS, PIK3CA and BRAF; an experiment result has high sensitivity and the mutation rate of 1 percent can be detected; the detection sensitivity is greatly improved and the difficulties in the prior art are overcome; meanwhile, requirements on large-batch and rapid detection are met through a whole experiment process.