The invention relates to a method for synthesizing an amino functionalized rare earth-doped lanthanum fluoride nano fluorescent marker material, which comprises the steps of: with phosphorylethanolamine as a surface active agent, mixing sodium fluoride, lanthanum nitrate and rare earth nitrate in distilled water, carrying out heat preservation at the temperature of 30-90 DEG C, and stirring for a period of time, washing and drying to obtain the aminated rare earth-doped lanthanum fluoride nanocrystalline with the component of xLn<3+>-(1-x) LaF3, wherein Ln<3+>=Ce<3+>, Yb<3+>, Er<3+>, Tm<3+>, Ho<3+>, Eu<3+>, Gd<3+>, Tb<3+>, Dy<3+>, Sm<3+>, Nd<3+> and Pr<3+>, and x=0-50mol%. The rare earth-doped LaF3 nano fluorescent marker material prepared by the method has the advantages that the size of nanoparticles can be controlled to be about 5nm, water solubility is good and the surface amino can be used to realize the connection with biomolecule. Furthermore, different rare earth ions can be doped into the nanoparticles, so that the specific fluorescence emission can be realized so that the biological connection can be detected sensitively; and the nano fluorescent marker material prepared by the method has the potential in application in the biological marking field.