6 results about "Specific fluorescence" patented technology

The present application relates to a kind of zinc-based coordination polymer and film preparation method and application fluorescent detection of sparteine.Polymer is three-dimensional zinc metal organic framework material, the frame is connected by binuclear zinc and organic ligand to have one-dimensional pore, chemical formula is {NH2(CH3)2·[Zn (TDA)]·DMF·3C2H5OH} n};With fluorescence emission peak at 400nm.It is prepared by hydrothermal reaction by zinc iodide and 4,4'‑(1H‑1,2,4‑triazole‑3,5‑diyl) dibenzoic acid in DMF and ethanol mixed solvent, add acetic acid as regulator.The zinc-based coordination polymer and film prepared by the present application have specific fluorescence recognition function, can quickly and conveniently detect sparteine in water and other solutions, the detection limit is as low as 5.26×10 ‑6 M, high sensitivity and can be recycled.And preparation process is simple, application portable, not dependent on expensive equipment.

This invention relates to the field of in vitro diagnostic technology and provides a highly specific Mycobacterium tuberculosis antigen detection kit and detection method. The kit includes: a thermosensitive antigen aggregate composed of two or more of ESAT-6, CFP-10, and MPT-64 coupled with PNIPAM-b-PLL block copolymer, which spontaneously forms nanoscale antigen clusters at 37°C; dual-mechanism anti-fouling magnetic beads, which are Fe3O4@SiO2@PEG-polysulfobetaine three-layer core-shell structures, with a reference fluorescent dye embedded in the SiO₂ layer via covalent bonding, and the surface covalently coupled to the thermosensitive antigen aggregate via carboxyl groups; an AIE fluorescent probe, composed of AIE fluorescent microspheres coupled with an anti-tuberculosis antigen detection antibody; and a sample diluent. This invention achieves local enrichment of antigen through temperature-controlled aggregation, minimizes background through dual-mechanism anti-fouling magnetic beads (PEG steric hindrance + zwitterionic super hydration), and amplifies the signal and performs internal reference calibration through AIE ratio fluorescence. The three components work synergistically to achieve high sensitivity, low background, strong anti-interference ability, and easy operation for tuberculosis antigen detection.

Disclosed are a recycled polypropylene composite material, and a preparation method therefor and a use thereof. The recycled polypropylene composite material comprises the following components calculated in parts by weight: 50-90 parts of a recycled polypropylene resin; 5-30 parts of polypropylene resin B; 0.1-0.8 parts of a rheological agent, the EPR content in the polypropylene resin B being not less than 28 wt%; the one-minute half-life temperature of the rheological agent being not higher than 210°C; and the fluorescence intensity of the recycled polypropylene resin at 380 nm-420 nm being 4000-6500. In the present invention, by compounding a rheological agent with a one-minute half-life temperature of not higher than 210°C and a polypropylene resin B with an EPR content of not less than 28 wt% into a recycled polypropylene resin with a specific fluorescence intensity, paint adhesion of the recycled polypropylene composite material can be effectively improved while good mechanical properties are maintained.

The application provides a cerebellar Purkinje neuron rapid identification system and method based on multi-modal deep learning, relates to the cross technical field of biomedical engineering and computer vision, and fuses specific fluorescence features and multi-dimensional morphological features, constructs a special multi-modal deep learning model, solves the interference problem of other neurons in a mixed culture system, and greatly reduces the false positive rate; a small sample learning architecture solves the industry pain point of a small amount of biological sample labeled data, improves the model generalization capability, and adapts to multi-scene sample identification. The identification system fuses Purkinje neuron specific fluorescence labeling features and cell morphological features, constructs a multi-modal deep learning recognition model based on small sample learning, is matched with a full-automatic microscopic imaging and analysis system, realizes rapid and high-accuracy identification of Purkinje neurons under a mixed culture system, does not need a complex cell purification step, greatly shortens the experimental period, and reduces the technical threshold.

The present application belongs to the technical field of gene detection, and aims at the fact that most of the existing methods focus on the specific report and multiplex detection capacity of pAgos, but often ignore the key problem of amplicon contamination. The present application provides a nucleic acid detection composition, kit and method for foodborne pathogens based on LAMP-PfAgo, which utilizes primers to perform LAMP amplification on foodborne pathogens, and after the reaction is completed, a probe and PfAgo are added, the PfAgo performs the first cutting on the LAMP amplification product to obtain a cutting product, and then the PfAgo performs the second cutting on the probe under the action of the cutting product to produce specific fluorescence. The primer probe combination of the present application can efficiently, specifically and sensitively detect multiple foodborne pathogens at the same time, and can effectively eliminate the problem of LAMP amplicon contamination.

This invention belongs to the field of biological detection and discloses a polarity and viscosity synergistic response fluorescent probe, its preparation method, and its application. The structural formula of the probe involved in this invention is as follows: This probe exhibits keto form D-A and enol form D in different polar solvents. + -A − The probe undergoes a structural transformation and is influenced by the photoinduced electron transfer (PET) effect. It exhibits no fluorescence in polar solvents and under low-polarity, high-viscosity conditions, but fluorescence is activated under high-polarity, high-viscosity conditions. In PBS buffer, the probe fluorescence is quenched due to the PET effect. Upon binding to HSA, the high steric hindrance microenvironment of HSA restricts probe molecule movement and inhibits the PET process, resulting in specific fluorescence activation at 578 nm. Furthermore, the fluorescence intensity shows a good linear relationship with HSA concentration over a wide range. This probe can rapidly and accurately detect albumin and can be used for detecting albumin levels in cerebrospinal fluid, applying it to the rapid screening of diseases related to blood-brain barrier damage.