75 results about "Mercuric ion" patented technology

The invention discloses a mercury ion detection fluorescent probe based on quinoline as well as a preparation method and application of the mercury ion detection fluorescent probe. The fluorescent probe and a structural formula of the fluorescent probe are shown as a formula I in the specification. A quinoline derivative, namely 2-methyl-7-hydroxyquinoline, is used as a fluorescent chromophore, a thioester group is used as a recognition group, and the prepared fluorescent probe shows high sensitivity and high selectivity on Hg < 2 + > in a solution. The preparation method of the fluorescent probe is simple in steps, raw materials are easy to obtain, and the obtained product is a white flocculent solid, is easy to store, has a wide application prospect and can be produced and applied on a large scale.

The application discloses a machine learning enhanced silver-based covalent organic framework nanoscale enzyme colorimetric detection method and application thereof, and constructs a covalent organic framework loaded with silver nanoparticles to form a nanoscale enzyme with oxidase-like activity, which is used for rapid visual detection of mercury ions. When mercury ions exist, silver ions in the COF-AgNPs can form silver amalgam with the mercury ions, thereby catalyzing the colorless substrate 3,3',5,5'-tetramethylbenzidine to generate a blue oxidation product oxTMB. The content of mercury can be judged by detecting the absorbance and RGB value changes of the color development reaction. Meanwhile, a machine learning data analysis strategy of a support vector machine model is adopted to perform five-fold stratified cross-validation analysis on the obtained absorbance and RGB values, so that the detection range is expanded and the detection sensitivity is improved.

The application relates to an organic polymer for rapid detection and degradation of mercury ions, and belongs to the technical field of preparation and application of organic functional materials. The structural formula of the organic polymer is shown as formula (IV); the luminescence performance of the polymer is derived from a conjugated organic polymer skeleton, and the recognition function for mercury ions is derived from a sulfur hydrocarbon chain embedded in the organic polymer skeleton. The polymer has high chemical stability and thermal stability, and can be repeatedly used for multiple cycles. The organic polymer is used for detecting and degrading mercury ions, and has the advantages of simple operation, low cost, short detection and degradation time, real-time detection, field detection and the like, and is easy to popularize and apply.

The invention discloses a machine learning enhanced silver-based covalent organic framework nano-enzyme colorimetric detection method and application thereof, and constructs a nano-enzyme which is formed by loading silver nanoparticles on a covalent organic framework and has oxide-like enzyme activity, and the nano-enzyme is used for rapid visual detection of mercury ions. When mercury ions exist, silver ions in the COF-AgNPs can form silver amalgam with the mercury ions, so that a colorless substrate 3, 3 ', 5, 5'-tetramethyl benzidine is catalyzed to generate a blue oxidation product oxTMB. And the mercury content can be judged by detecting the absorbance and RGB value change of the chromogenic reaction. And meanwhile, a machine learning data analysis strategy of a support vector machine model is adopted to carry out five-fold layered cross validation analysis on the obtained absorbance value and RGB value, so that the detection range is expanded, and the detection sensitivity is improved.

The present application mainly relates to a kind of preparation of sulfur-rich amino acid protein CMd-copper nanoclusters and its detection mercury ion method, comprising: a) by prokaryotic expression obtains CMd protein, and the protein concentration of 5 mg / mL-25 mg / mL CMd protein solution is obtained by purification.b) CMd is reacted with reducing agent TCEP after opening protein disulfide bond.c) the protein solution after reduction is reacted with anhydrous copper sulfate, and preparation high fluorescence CMd-copper nanocluster.d) CMd-copper nanocluster is applied to detect heavy metal mercury ion.The application method can be synthesized under mild aqueous conditions by "one-step method" structure controllable, stable CMd-copper nanocluster, widens the raw material of copper nanocluster synthesized with protein as template, avoids the use of organic solvent and strong reducing agent, and reduces the cost and environmental burden brought by noble metal raw material, and also has higher selectivity to mercury ion.

The application belongs to the technical field of contaminated soil remediation, and particularly relates to modified montmorillonite for synergistically remediating heavy metals and organic pollutants in soil as well as a preparation method and application thereof. Hexadecyl trimethyl ammonium bromide (CTAB) and dodecyl trimethyl ammonium bromide (DTAB) are used as modifiers to prepare organic modified montmorillonite, and mercury ions (Hg 2+ ) and acetochlor (ACR) are used as representative substances of heavy metals and organic pollutants to study the effects of the two kinds of organic modified montmorillonite on remediation of Hg 2+ and ACR compound contaminated soil. The results show that the two kinds of organic modified montmorillonite have good removal effects on Hg 2+ and ACR in real soil samples, and can simultaneously remove Hg 2+ and ACR in soil. The removal rates of CTAB modified montmorillonite and DTAB modified montmorillonite can both reach more than 90%. The organic modified montmorillonite prepared by using CTAB and DTAB as modifiers can simultaneously remove Hg 2+ and ACR in soil, and can synergistically remediate Hg 2+ and ACR compound contaminated soil.

The invention discloses an Hg < 2 + > detection method for enhancing Hg < 2 + > intrinsic laccase-like activity based on ZIF-8-Au. The invention finds that the mercury ions have laccase-like activity, the enzyme activity of the mercury ions is obviously enhanced under the action of ZIF-8-Au, and the color of the reaction catalyzed by the mercury ions is obviously changed along with the increase of the concentration of Hg < 2 + >, so that a colorimetric analysis method for detecting the mercury ions is developed on the basis, the Turn-On type visual analysis is realized, and the application prospect is wide. The method realizes simple, rapid, sensitive and high-selectivity detection of Hg < 2 + > residues in water and food. The invention provides a new method and principle for the fields of Hg < 2 + > environment monitoring and food safety, and provides a new direction for the development of a heavy metal ion detection technology.

This invention belongs to the field of wastewater treatment in metal mines and proposes a coupled method for removing ultra-low concentration mercury from metal mine wastewater. It employs a combined process of "coupled chemical precipitation + flocculation precipitation + sulfur-loaded activated carbon adsorption." First, stannous chloride is added to generate a hydroxide gel, which is then chemically precipitated in conjunction with amorphous FeS nanoparticles. Next, hydrophobically modified cationic polyacrylamide is used for flocculation precipitation. Finally, modified sulfur-loaded activated carbon is used for deep adsorption. This treatment process has a specific removal effect on mercury ions, and the final effluent mercury meets the Class II surface water standard, with stable effluent quality. It also solves the problem of sulfur loss from ordinary sulfur-loaded activated carbon.

The present invention discloses a benzothiadiazine-1,1-diketo-quinoline derivative, a preparation method, and an application thereof, belonging to the technical field of chemical optical materials. A benzothiadiazine-1,1-diketo-quinoline derivative is provided. The structural formula of the benzothiadiazine-1,1-diketo-quinoline derivative is: wherein R is -N(CH2CH3)2 or -OCH3. The present invention introduces a benzothiadiazine-1,1-diketo heterocycle at the 2-aldehyde position of quinoline to prepare the benzothiadiazine-1,1-diketo-quinoline derivative. The preparation cost is low, the operation is simple, and the method is simple. The obtained benzothiadiazine-1,1-diketo-quinoline derivative exhibits fluorescent properties and can be used as a probe for the detection of heavy metal copper and / or mercury ions. At the same time, the synthesis method can be used for synthesis in fine organic chemical fields such as printing and dyeing technology and analytical detection.

The invention belongs to the field of mercury ion detection, and provides a mercury ion detection electrochemical sensor, a preparation method thereof and a mercury ion detection method. The mercury ion detection electrochemical sensor is composed of a basic electrochemical electrode, a vertical mesoporous silica nano-channel film uniformly deposited on a sensing interface of the basic electrochemical electrode, and mercury ion recognition molecules connected to the nano-channel film, the mercury ion recognition molecules are connected to the surface of the nano-channel membrane and in the nano-channel through a silane coupling agent; the mercury ion recognition molecule is 1-(6-(hydroxymethyl)-2, 2-dimethyl tetrahydrofuran [3, 4-d] [1, 3] dioxacyclo-4-yl)-5-methylpyrimidine-2, 4 (1H, 3H)-diketone. According to the invention, the specific recognition capability of the existing electrochemical sensor on the mercury ions can be enhanced, the preparation difficulty and the preparation cost are reduced, and convenient, low-cost, high-efficiency and high-selectivity detection of the mercury ions is realized.

This invention discloses a fluorescent sensitive membrane for detecting mercury ions in water and its preparation method. The sensitive membrane comprises a fluorescent probe and a physically cross-linked matrix. The fluorescent probe is prepared using 7-(diethylamino)coumarin-3-carboxaldehyde, 3-methyl-2-benzothiazolidinone hydrazone hydrochloride, and ethanol. Chitosan, glycerol, and acetic acid solutions are then added to a round-bottom flask and stirred until the chitosan is completely dissolved. Next, 4-(4,6-dimethoxytriazine-2-yl)-4-methylmorpholine hydrochloride is added, and stirring continues at room temperature to obtain a clear and transparent solution. The fluorescent probe is then added, and stirring continues. Finally, the solution is poured into a glass dish to evaporate the solvent, yielding the mercury ion detection solution. 2+ A fluorescently sensitive membrane. This membrane is sensitive to Hg in water. 2+ It has a detection function, requires no sample preparation during detection, and has the advantages of simple detection method and high detection efficiency.

The application aims to provide an application of a ratio fluorescent probe based on gold nanoclusters / long afterglow nanorods in mercury ion detection, and belongs to the technical field of fluorescent detection, which comprises synthesis of gold nanoclusters, preparation of long afterglow nanorods, and preparation and application of the ratio fluorescent probe. The ratio fluorescent probe comprises red light emitting gold nanoclusters and green light emitting long afterglow nanorods, and the fluorescent emission of the two materials has obvious spectral and color distinction. The responses of the gold nanoclusters and the long afterglow nanorods to mercury ions are completely opposite, mercury ions can quench the luminescence of the gold nanoclusters, while the luminescence of the long afterglow nanorods is enhanced to a certain extent. The response difference of the two fluorescent signals to different concentrations of mercury ions is utilized, and the ratio processing of the fluorescent intensity can be used for ratio fluorescent detection of mercury ions, and obvious composite fluorescent color change can be observed under the same excitation light irradiation, which is convenient for visual analysis of mercury ions.

The invention discloses an intrinsic micropore degradable polymer material based on biomass and hetero-calixarene and a preparation method and application thereof.The method comprises the steps that firstly, cyanuric chloride and resorcinol serve as raw materials, and oxygen atom bridged hetero-calixarene macrocyclic molecules are synthesized in one step under the alkaline condition through a one-pot method; then introducing the lignin derivative into oxygen atom bridged hetero calixarene by utilizing an aromatic nucleophilic substitution reaction on a triazine ring in a macrocyclic molecule, and carrying out a condensation reaction on the obtained multi-aldehyde functionalized oxygen atom bridged hetero calixarene and polyfunctional mercaptan under an acidic condition to obtain the target degradable polymer material. When in use, the material is directly put into an aqueous solution of elemental iodine, iodine negative ions and divalent mercury ions for adsorption. The removal rate of the material to low-concentration iodine simple substances, iodine negative ions and divalent mercury ions can reach 90% or above, and the material after adsorption can be repeatedly used after being washed by an acidic aqueous solution. The material can be degraded under an alkaline condition.

The invention relates to a mercury-containing acid mud efficient recovery method and device based on difference frequency resonance coupling. The high-efficiency mercury-containing acid mud recovery method based on difference frequency resonance coupling comprises the following steps: step 1, acid mud pretreatment: crushing, screening and drying mercury-containing acid mud; 2, coupled field leaching is conducted, specifically, a product in the step 1 is conveyed into a reaction kettle, stirring is conducted after an oxidizing agent is added, an acid mud turbid liquid is obtained, an oxidation-reduction reaction is conducted through catalysis of a difference frequency resonance coupled field formed by two frequency-adjustable ultrasonic waves, and the difference frequency adjusting range delta f is 6-10 kHz; step 3, carrying out solid-liquid separation; leaching liquid containing mercury and selenium is obtained. According to the scheme provided by the invention, the standing wave effect can be formed through the difference frequency, and the crushing efficiency of the cavitation bubbles is improved, so that the oxidation reaction speed of the mercury-containing acid mud turbid liquid is improved; secondly, through the adjustable frequency, the situation that the ultrasonic frequency is not matched with the mercury ion concentration and the turbid liquid temperature, and consequently ultrasonic energy waste is caused is avoided.

The invention relates to a cyano-stilbene mercury ion fluorescent probe compound and a preparation method thereof. The molecular structure of the compound is shown in the specification.

The invention belongs to the technical field of ion detection, and particularly relates to a preparation method of a ratio type fluorescent probe for detecting collected ions, which comprises the following steps: dissolving ethylenediamine and di-tert-butyl dicarbonate in perchloroform to obtain a product 1; dissolving the product 1 in absolute ethyl alcohol, and adding 4-Australio-1, 8-dianhydride to obtain a product 2; dissolving the product 2 in thousand dimethyl sulfoxide, and adding N-isothiazolyl phthaloyl to obtain a product 3; dissolving the product 3 in dichloromethane, and adding phenyl thiochloroformate to obtain the fluorescent probe NAP. The preparation method comprises the following steps: dissolving a fluorescent probe NAP in DMSO to prepare a mother liquor A, and dissolving methoxy chitosan CS-MeO in water to prepare a mother liquor B; and mixing the mother liquor A and the mother liquor B to prepare a Nano-NAP solution. The ratiometric fluorescent probe is used for detecting collected ions, has the advantages of high selectivity, small interference, low detection limit, good pH stability and the like, is high in use value, and has a wide application prospect.

The application discloses a pyrrole ring-containing boron-sulfur doped fused ring aromatic hydrocarbon and a synthesis method and application thereof, and relates to a pyrrole ring-containing boron-sulfur doped fused ring aromatic hydrocarbon which is synthesized from simple raw materials such as 2,6-difluoronitrobenzene through a Clauson-Kaas pyrrole synthesis reaction and an electrophilic boronation reaction. Property research involves ultraviolet, fluorescence and CV tests, and mercury ion tests. The application has the characteristics of a short reaction path, a simple operation method and mild reaction conditions, and the compound has the potential of application in the field of photoelectric devices and can also be applied to mercury ion identification.

The invention relates to the technical field of chemical hazardous substance detection, in particular to a Tb-CeMOF (at) ATP fluorescent probe as well as a preparation method and application thereof. The fluorescent probe is obtained by taking a terbium / cerium bimetal organic framework Tb-CeMOF as a matrix and an aptamer as a recognition element and modifying the Tb-CeMOF framework with the aptamer through synthesis. The detection mechanism is as follows: when mercury does not exist, the aptamer enables the characteristic fluorescence of terbium to be excited. When a target object exists, the aptamer is specifically combined with mercury, and conformational change causes change of an energy transfer path: the characteristic fluorescence of terbium is quenched; characteristic fluorescence of cerium is obviously enhanced, and an'on-off-on 'type ratio fluorescence signal is formed. A standard curve is established by monitoring the linear relation between the ratio change of the fluorescence intensity and the mercury ion concentration, and accurate quantification of the mercury ion residual quantity in the sample is achieved. By means of simple operation and excellent sensitivity and selectivity, a reliable and efficient new strategy is provided for trace detection of mercury ions.

The invention relates to the technical field of fluorescent probes for metal ion detection, in particular to a coumarin mercury ion ratio fluorescent probe for targeting mitochondria as well as preparation and application of the coumarin mercury ion ratio fluorescent probe. The ratiometric fluorescent probe is applied to rapid detection of mercury ions and targeted imaging of mercury ions and mitochondria in living cells. At present, reported mercury ion fluorescent probes still have some limitations, such as complex synthesis, separation and purification, single-wavelength detection, no targeted positioning function and the like. The preparation method of the ratiometric fluorescent probe comprises the following steps: carrying out a reaction on O-(4-(bromomethyl) phenyl) diphenyl selenophosphonate and 7-diethylamino-3-(4-pyridyl) group coumarin in acetonitrile to generate 4-(7-(diethylamino)-2-oxo-2H-chromene-3-yl)-1-(4-((diphenyl selenophosphoryl) oxy) benzyl) pyridine-1-ammonium bromide. The fluorescent probe disclosed by the invention is simple to prepare, separate and purify, and fluorescent probe molecules have the advantages of good selectivity, high sensitivity, high response speed and the like in a coexistence system of mercury ions and other metal ions.

This invention provides a highly selective and sensitive fluorescent probe for mercury ions, its preparation method, and its applications, belonging to the fields of chemical sensing materials and environmental monitoring technology. This invention connects 4-(1,2,2-triphenylvinyl)phenol, which exhibits aggregation-induced emission, and a guanosine derivative with multiple coordination sites, via a flexible chain to obtain a fluorescent probe with the structure shown in Formula I. This fluorescent probe not only overcomes the problems of large differences in solubility and reactivity between 4-(1,2,2-triphenylvinyl)phenol and guanosine derivatives, making coupling difficult, but also possesses high sensitivity and selectivity; its detection limit reaches 12.24 nM, effectively detecting trace mercury pollution in samples. It can also accurately identify mercury ions in water samples containing multiple high concentrations of metal ions, demonstrating high accuracy in real water samples. It is suitable for the accurate determination of mercury ions in complex environmental water samples and has good practical application value in the field of environmental monitoring. Formula I

The invention provides a multifunctional nano material Ni-MoS2 (at) Au for detecting trace toxic substances in mushrooms and a preparation method of the multifunctional nano material Ni-MoS2 (at) Au. The nano material is prepared from a metal oxide, a surfactant, precious metal nano particles and a solvent, the nano material has colorimetric and Raman detection functions on various trace toxic substances (mercury ions and amanita peptide) in mushrooms, hydrogen peroxide is immediately catalyzed to quickly oxidize 3, 3 ', 5, 5'-tetramethyl benzidine into yellow 3, 3 ', 5, 5'-tetra (tetrafluoromethyl) phenyl diamine oxide after the nano material is in contact with a target object Hg < 2 + > for 1s in a colorimetric mode, the nano material has an extremely low detection limit of 0.036 mu g / mL, and the nano material can be used for detecting various trace toxic substances (mercury ions and amanita peptide) in mushrooms. The catalyst has good selectivity and is not interfered by other heavy metal ions; in a Raman mode, high-sensitivity detection of alpha-amanita peptide and beta-amanita peptide can be realized, and the detection limit ranges are 10 <-4 > M to 10 <-8 > M. The nano material provides a reference mode capable of detecting multiple target objects in the same system, and opens up a new way for a detection strategy of trace toxic substances in the field of food safety detection.

The invention relates to the field of heavy metal ion detection, in particular to a preparation method and application of a mercury ion colorimetric and ratiometric fluorescent probe based on carbazole. The preparation method of the colorimetric and ratiometric fluorescent probe comprises the following step: carrying out reaction on O-(4-(bromomethyl) phenyl) diphenyl selenophosphonate and (E)-9-ethyl-3-(2-(quinoline-4-yl) vinyl)-9H-carbazole in acetonitrile to generate (E)-1-(4-((diphenyl selenophosphoryloxy) benzyl)-4-(2-(9-ethyl-9H-carbazole-3-yl) vinyl) quinoline bromide. The fluorescent probe has the advantages of simple synthesis route, strong operability, easily available raw materials and low cost, can realize rapid detection of mercury ions by using colorimetric and change of the ratio F528nm / F648nm of fluorescence intensities at two different emission wavelengths, and has the advantages of large Stokes shift, high sensitivity, good selectivity and the like.

This invention provides an application of a naphthalenepyridone fluorescent probe in the detection of mercury, iron, and iodide ions, belonging to the field of fluorescence analysis technology. Different metal cations were added to the CH3CN / H2O solution containing the fluorescent probe, and it was found that only Hg was detected. 2+ This can enhance the fluorescence of the fluorescent probe, while Fe... 3+ The addition of [a specific ingredient] can quench the fluorescence of the fluorescent probe. Furthermore, [the following is an example of a specific ingredient]: A3-Hg. 2+ When different anions were added to a CH3CN / H2O solution containing Ion, it was found that only Ion... ‑ The addition of A3-Hg can make 2+ The fluorescence is weakened, therefore this fluorescent probe can recognize Fe with high sensitivity and high selectivity. 3+ Hg 2+ and I ‑ In addition, this fluorescent probe can also be used to treat Hg in traditional Chinese medicine. 2+ Quantitative detection.

The invention relates to a near-infrared ratiometric fluorescent probe for mercury ions of targeted mitochondria as well as preparation and application of the near-infrared ratiometric fluorescent probe. According to the preparation method of the fluorescent probe, (E)-4-(2-(7-(diethylamino)-2-oxo-2H-chromene-3-yl) vinyl)-1-(4-((diphenylselenophosphoryl) oxy) benzyl) is obtained by taking (E)-7-diethylamino-3-(4-quinolyl) vinyl coumarin, diphenylselenophosphoryl and p-hydroxybenzyl as fluorophore, recognition group and connecting arm respectively, and (E)-4-(2-(7-(diethylamino)-2-oxo-2H-chromene-3-yl) vinyl)-1-(4-((diphenylselenophosphoryl) oxy) benzyl) quinoline-1-bromide, namely a compound 1; according to the application of the fluorescent probe, mercury ions are detected by utilizing the change of the fluorescence intensity ratio F721nm / F578nm at two different emission wavelengths, and the fluorescent probe is used for targeted imaging of mercury ions and mitochondria in living cells. The fluorescent probe is simple to prepare, and has the advantages of good selectivity, high sensitivity, high response speed and the like in a coexistence system of mercury ions and other metal ions.

The invention discloses a fluorescent probe for detecting metal mercury ions and a preparation method and application thereof, the molecular formula of the probe is C21H20O5N2S, and the structural formula is shown in the specification. The invention designs a fluorescent probe for detecting metal mercury ions (Hg < 2 + >) aiming at environmental Hg < 2 + > monitoring requirements. The probe can rapidly react with Hg < 2 + > (response time is 1t, 10 s), and has nanomole-level detection sensitivity (detection limit is 1.0 nM) and excellent anti-interference capability. The method is applied to detection of mercury ions (Hg < 2 + >) in real water samples, crop soil, plant root systems and in-vitro living cells. And a novel tool is provided for providing intermediate information for pathological research of Hg < 2 + > related diseases and evaluating environmental toxicity.

The present invention discloses a cationic fluorescent probe for antibacterial applications, as well as its preparation method and application. The structural formula of the fluorescent probe is as follows: First, E-dU is prepared; then, TPEPy-N3 is prepared; and finally, the E-dU and TPEPy-N3 are reacted via a click reaction to obtain the fluorescent probe. This fluorescent probe effectively addresses solubility and biocompatibility issues and possesses multiple functions, including mercury ion detection, glutathione response, cell imaging, and bacterial killing.

The present invention discloses a preparation method and application of a sulfur-rich thienyl covalent organic polymer material, belonging to the technical fields of organic functional materials and heavy metal adsorption. The present invention successfully prepares a sulfur-rich thienyl covalent organic polymer by a simple solvothermal method, utilizing the monomers benzo[1,2-b:3,4-b':5,6-b"]terthiophene-2,5,8-tricarbaldehyde and the monomer pyrazine-2,5-diazidine amine aldehyde condensation. The polymer can serve as an effective adsorbent for mercury ions. The sulfur-rich thienyl covalent organic polymer material can remove mercury ions within a pH range of 3-7, with a maximum adsorption capacity of 170.6 mg g ‑1 The synthesis method of the present invention requires simple equipment, the chemical reagents used are easily available, the synthesis conditions are mild, the product structure is stable and not easily hydrolyzed, the industrial application value is high, the practical application is effective, and it is easy to promote and apply.

The application discloses a mercury ion detection method and device and a preparation method of a nano-gold Raman enhancer, and is used for improving the detection accuracy of heavy metal mercury ions. The mercury ion detection method comprises the following steps: preparing a nano-gold Raman enhancer by using a chloroauric acid solution, a sodium citrate solution and a thiuram solution; adding the nano-gold Raman enhancer into a mercury ion reference solution; performing Raman spectrum detection on the mixture to generate a spectrum graph; determining a target characteristic peak from the spectrum graph as a discrimination basis for enhancing Raman scattering spectrum detection of mercury ions, and determining that the mercury ion concentration and the intensity of the characteristic peak are in a positive correlation relationship, the mercury ion reference solution is a solution with a known mercury ion concentration; adding the nano-gold Raman enhancer into a sample solution to be detected; performing Raman spectrum detection on the mixture, and calculating the mercury ion concentration in the sample solution to be detected according to the peak area of the known characteristic peak.

The present application relates to the field of optical sensing imaging detection technology, and specifically to a preparation method and application of an indazole-modified rhodamine mercury ion probe. The present application provides a compound, and a salt or solvate thereof, the chemical structure of the compound being as shown in Formula I: #imgabs0# wherein R1 and R2 are independently selected from C1 to C2 alkyl groups. The present application also provides a crystalline form, and the compound shown in Formula I and / or the crystalline form can be used to prepare an indazole-modified rhodamine mercury ion probe. Under certain conditions, the indazole-modified rhodamine mercury ion probe can achieve high selectivity, high sensitivity, in-situ, and real-time response to mercury ions, and the detection limit can reach 10 ‑10 The order of magnitude of M; its preparation method is simple and easy with low cost; a molecular fluorescent probe that can be used to detect mercury ions is designed using the dye as an optical signal reporter group.

The invention provides a preparation method of a phenylboronic acid functionalized silicon quantum dot fluorescent probe and application of the phenylboronic acid functionalized silicon quantum dot fluorescent probe in detection of mercury ions and methyl mercury ions, and relates to the technical field of detection of heavy metal mercury ions and methyl mercury ions. In the presence of mercury ions (Hg < 2 + >) and methyl mercury ions (CH3Hg < + >), silicon point fluorescence is quenched. By combining ultraviolet portable equipment and combining a silicon puncture-Hg < 2 + > and silicon puncture-CH3Hg < + > system with RGB value acquisition and conversion of a smart phone platform, rapid and high-sensitivity detection of mercury ions and methyl mercury ions in an environmental water body is realized. The detection method disclosed by the invention has the advantages of simplicity and convenience in operation, rapid feedback, high sensitivity, good repeatability, high stability, strong accuracy and the like, can intuitively realize rapid determination of mercury ions and methyl mercury ions in a water sample in real time, and widens the application direction of silicon dots in the aspect of environmental detection.