454 results about "Mercuric ion" patented technology

The invention relates to a preparation method for a thiol-functionalization MOFs material and application thereof in adsorption and removal of heavy metal ions in water. The thiol-functionalization MOFs (UiO-66-SH) material can effectively adsorb and remove the ions of heavy metal such as copper, cadmium, lead, mercury in the water. The method comprises the steps that 1, thiol-modified terephthalic acid organic ligands are synthesized; 2, the thiol-functionalization MOFs material (UiO-66-SH) is synthesized through a solvothermal method; 3, the thiol-functionalization MOFs material (UiO-66-SH) serves as an adsorbing agent to be applied to adsorption and removal for the heavy metal ions such as copper, cadmium, lead, mercury in the water. The thiol-functionalization MOFs material can efficiently adsorb and remove the ions of heavy metal such as copper, cadmium, lead, mercury in the water, and compared with ordinary UiO-66, the adsorption property of the material is significantly improved; in addition, the material can selectively adsorb and remove mercury ions on the high-concentration condition, and novel application of the material is widened.

The invention relates generally to gold nanoclusters, and in particular, fluorescent gold nanoclusters. The gold nanoclusters may be stabilized, for example, with a protein or stabilizing agent. In some cases, the gold nanoclusters may be used in methods or articles to determine the presence, absence, and / or concentration of mercuric ions in a sample.

The invention discloses a sulfide functionalized covalent organic frame material, The material has a structural unit shown in the specification, and is obtained by the following steps: adding 2,5-Bis(3-(ethylthio)propoxy)-terephthalohydrazide and benzenetricarboxaldehyde into a mixed solvent of 1,4-dioxane and mesitylene, adding an aqueous acetic acid solution into the above mixture and reacting at 120 DEG C for 1-3 days. The material has a mesoporous structure, has BET specific surface area of 470-480 m<2>?g<-1>, has good selective identification for mercury ions, and can be used for removal of the mercury ions.

The invention provides a gold nanocluster preparation method and an application thereof, and belongs to the technical field of nano materials. The problem of high cost of gold nanoparticle compositing in the prior art is solved. The gold nanocluster preparation method includes the steps: a, mixing polypeptide water solution with HAuCl4 solution in a reaction vessel to prepare solution A; and b, adding NaBH4 solution into the solution A for reduction, and reacting for a certain time to obtain fluorescent gold nanocluster products. A series of gold nanoclusters with different maximum emission wavelengths are composited successfully by means of taking different polypeptides as templates and sodium borohydride as a reducer. The composited gold nanoclusters have the advantages of large Stokes shift, long fluorescence lifetime, good stability and the like, can be used for detecting mercury ions (Hg2+) in water samples, and is high in detection sensitivity.

The invention discloses a preparation method for a Ru(bpy)3<2+>-gold nanoparticle-DNA-ferrocene functionalized graphene modified glassy carbon electrode. The method comprises the following concrete steps: polishing of a glassy carbon electrode; coating of Ru(bpy)3<2+> to prepare a Ru(bpy)3<2+> modified glassy carbon electrode; coating of gold nanoparticles to prepare a Ru(bpy)3<2+>-gold nanoparticle modified glassy carbon electrode; coating of single-stranded DNA to prepare a Ru(bpy)3<2+>-gold nanoparticle-DNA modified glassy carbon electrode, wherein the sequence of the single-stranded DNA is rich in T bases, a stable double-stranded DNA structure can be formed after bonding with Hg2+, and the 5' end of the sequence of the single-stranded DNA is connected with an SH-(CH2)6 group; and coating pf ferrocene functionalized graphene so as to prepare the Ru(bpy)3<2+>-gold nanoparticle-DNA-ferrocene functionalized graphene modified glassy carbon electrode. The modified glassy carbon electrode prepared in the invention can be used for detecting the content of mercury ions in a water sample and has good electrochemiluminescence performance, excellent stability and reproducibility and high sensitivity; and operation of the method is easy and convenient.

The invention relates to a wet ammonia flue gas purification process for the combined sulfur and mercury removing and the system thereof. The process adopts chlorine gas as oxidizing agent to completely oxidize elemental mercury in the flue gas, and gas phase divalent mercuric ion after being oxidized is converted into liquid phase divalent mercuric ion through the improved wet ammonia desulphurization process, and further the liquid phase divalent mercuric ion is converted into stable mercuric sulfide sendimentation through liquid phase divalent mercuric ion stabilizing agent. The system comprises a flue gas oxidizing demercuration preprocessing system composed of an oxidizing agent injection device and an electrostatic precipitator, a wet ammonia flue gas sulfur and mercury removing system composed of a reaction absorbing tower, a sulfur removing accessory substance processing system composed of a cyclone cleaner, a dewaterer, and a dryer, and a mercuric sulfide HgS sendimentation processing system composed of a wastewater buffer pool, a liquid phase mercuric ion reaction chamber, and a settling pond. The process and the system can effectively improve the oxidation rate of the elemental mercury in the flue gas, overcome the defaults that the divalent mercuric ion is easy to be reduced and released and the secondary pollution problem is caused by excess oxidizing agent, and greatly improve the efficiency of sulfur removing and mercury removing.

The invention discloses a mercury-ion detection method simulating peroxidase based on nano platinum and a kit. After the specificity interaction of the nano platinum and mercury ions, the inhibition variation of the activity of the perioxidase is simulated, hydrogen peroxide is catalyzed by the nano platinum to oxidize 3, 3', 5, 5'-TMB HCL to achieve color developing. The invention provides a novel rapid, simple and ultrasensitive mercury ion detection method. The nano platinum used in the detection method is simple to prepare and easy to obtain, and visualized and convenient analysis on the mercury ions can be realized. The detection method has the advantages of simplicity in operation, short detection time, high sensitivity, high specificity and the like and is easy to popularize and use.

The invention relates to the field of sewage treatment, in particular to a treatment method of mercury-polluted water. The mercury-polluted water treatment process mainly comprises pretreatment and mercury removal, wherein the pretreatment is as follows: pretreatment is carried out on the collected polluted water, the suspended matter concentration in the water body is less than or equal to 100mg / L; mercury removal is as follows: (1) inorganic mercury is removed; (2) mercury and mercuric salt are removed by adsorption; secondary adsorption is carried out by an obligate zeolite and hydroxide radical activated carbon, wherein the hydroxide radical activated carbon further adsorbs inorganic mercuric salts in the polluted water; (3) mercury and mercuric salts are removed by ion exchange, and final treatment is carried out on mercury in the polluted water, so as to enable mercury ions in the treated polluted water to reach mercury discharge limit value 0.5mug / l. In the invention, the process is simple, organic mercury and inorganic mercury in the polluted water can be thoroughly removed effectively, so as to enable the mercury-containing concentration to reach emission standards.

The invention discloses a method for recycling a waste mercury catalyst. The method comprises the steps of adding the waste mercury catalyst into water, controlling the temperature at 70-95 DEG C, carrying out hot dissolution pretreatment while stirring, regulating the pH value to 1-3, and filtering after carrying out ultrasonic treatment for 10-60min or filtering in an ultrasonic field to separate mercuric chloride and phosphorus and sulfur impurities from activated carbon in time, wherein the mercuric chloride and the phosphorus and sulfur impurities are separated from the surface of the activated carbon; after filtering, washing the obtained activated carbon and sieving to prepare a mercury catalyst; controlling the temperature of mercury-contained filtrate at 50-90 DEG C, adding a neutralizing agent to regulate the pH value to 6-8, and reacting for 30-90min, wherein mercury ions in the solution are completely converted into mercuric oxide sediments, and toxic substances, namely phosphorus and sulfur in the mercury catalyst still remain in the solution; filtering to obtain a mercuric oxide filter cake, and dissolving with hydrochloric acid to obtain a mercuric chloride solution for preparing the mercury catalyst. According to the invention, the waste mercury catalyst is recycled through acidification-ultrasonic synergistic desorption, coupling and filtration, therefore, the method is simple in process, high in efficiency and low in energy consumption.

The invention discloses a mercury ion detection method which is characterized by comprising the following steps: (1) mixing a standard mercury ion solution and a gold nanocluster solution, and establishing a standard curve for detecting the fluorescence signal quenching of nanocluster and the mercury ion quantity; and (2) mixing a sample solution with the gold nanocluster solution, substituting the quenching intensity of the fluorescence signal of the nanocluster into the standard curve, thereby obtaining the content of the mercury ion in the sample. The method has the advantages that the sensitivity is high, the interference of other ions on detection of the mercury ion is slight, and selective detection of the mercury ion can be realized, so that the aim of rapidly detecting the mercury ion concentration is achieved. The method has wide application prospects in mercury ion detection.

The invention discloses a pillar 5 arene polymer with fluorescence detection and mercury ion removal functions. The pillar 5 arene polymer is obtained through steps of carrying out reflux reaction on copolymerized pillar 5 arene and ethyl thioglycolate as raw materials to obtain functionalized pillar 5 arene carrying out reflux reaction on the functionalized pillar 5 arene and hydrazine hydrate as the raw materials to obtain pillar 5 arene; enabling the pillar 5 arene to react with terephthalaldehyde to obtain a yellow-green sediment; and filtering, washing and drying to obtain the pillar 5 arene polymer. According to the polymer, a good fluorescent signal base is provided for a benzene ring group of the pillar 5 arene, yellow-green fluorescent light of the polymer is quenched through complexing a sulphur atom and a mercury ion, and fluorescent recognition of the mercury ion is achieved. The polymer has the characteristic of being difficult to dissolve into a water solution and an organic solution, and the mercury ion can be separated from a water phase, thereby achieving the purpose of removing the mercury ion.

The invention discloses a mono-sulfo squarylium dye fluorescence probe for detecting mercury ions and a preparation method thereof as well as application thereof. The preparation method comprises the following steps of: proportionally mixing oxo squarylium essence and a Lawesson's reagent, dissolving the mixture in methylbenzene, performing backflow reaction for 1.5-4 hours under the protection of inert gas after the mixture is dissolved, cooling the reaction mixture to room temperature, decompressing and distilling the reaction mixture to remove a solvent and obtain a rough product; and performing silica gel column chromatography separation on the rough product to obtain the mono-sulfo squarylium dye fluorescence probe. The mono-sulfo squarylium dye fluorescence probe provided by the invention is high in stability and can quickly, sensitively and specially detect low-concentration mercury ions; a synthesis method is simple; and the production cost is low.

The invention provides a method for preparing an adsorbent for removing mercury ions, lead ions, and other heavy metal ions from waste water. The method comprises the following steps of: dissolving a silane coupling agent KH-550 in alcohol, and then adding a certain quantity of roasted mesoporous molecular sieves MCM-41; refluxing for 6-12 hours in a water bath at 60-80 DEG C; centrifuging and separating a product, and then washing with alcohol; and drying in vacuum at 40-80 DEG C to obtain the aminopropyl functional MCM-41. The invention has the characteristics of simple process, low raw material price, safety and low required equipment cost. The adsorbent has a three-dimensional pore canal structure and has excellent adsorption effect on the mercury ions and the lead ions in the waste water.

The invention provides a rate type fluorescent probe used for mercuric ion detection. The probe is formed by dual-emitter nanometer material gold / silver nanoclusters wrapped by bovine serum albumin, and the fluorescence-emission wavelength is 400-550 nm and 580-680 nm. The dual-emitter nanometer material gold / silver nanoclusters are synthesized through a one-pot method by using the bovine serum albumin (BSA) as a reducing agent and a stabilizer. The synthesized gold / silver nanoclusters (Au / Ag NCs) have two emission peaks, wherein Ag NCs serves as a reference signal Au NCs serves as a responsesignal and is used for selective recognition of Hg2+. When the dual-emitter gold / silver nanoclusters serve as the rate type fluorescent probe, the Ag NCs fluorescent signal intensity is kept unchangedbasically, and Au NCs is selectively combined with Hg2+ to cause fluorescence quenching. The method is quick, simple, low in detection limit, wide in linear range and high in selectivity.

The invention relates to a preparation method of a mercury ion absorbent and application of the mercury ion absorbent, and belongs to the field of industrial wastewater treatment. A preparation method of a sulfonated grapheme / polyaniline composite is characterized by comprising the following steps: preparing sulfonated grapheme by utilizing natural graphite through a chemical oxidation method; 2) dispersing and dissolving the sulfonated grapheme into water, and adding polyaniline to obtain solution A, wherein the concentration of the sulfonated grapheme is 1-2 mg / mL, and the concentration of the polyaniline is 0.02-0.5 mol / L; 3) dissolving ammonium persulfate into hydrochloric acid to obtain a molar ratio of ammonium persulfate to polyaniline of 0.5:1-2:1 so as to obtain hydrochloric acid solution of ammonium persulfate; 4) adding the hydrochloric acid solution of ammonium persulfate into the solution A, and stirring and reacting for 6-24 hours; and 5) filtering a product obtained in the step 4), washing until filtrate is colorless, and drying in vacuum to obtain the sulfonated grapheme / polyaniline composite. The preparation method, provided by the invention, has the characteristics of low cost and good absorption performance on mercury ions.

The invention discloses a colorimetric analysis method for simply and rapidly detecting mercury ions in an aqueous solution. According to the method, the characteristic of high light absorption coefficient of nanogold is utilized, the nanogold is used as a chromogenic signal component, and simultaneously mercaptoethylamine is used as a specificity identification component. The colorimetric analysis method has the advantages of being low in cost, simple in operation, convenient and rapid. The modified nanogold is stable, high in resistance to high concentration salt ions, slightly interfered by external factors and suitable for being popularized and applied.

The invention relates to a fluorescence gold nanoparticle and a preparation method thereof. The fluorescence gold nanoparticle is prepared according to a method including the following steps: mixing an egg white water solution, a gold salt water solution and a sodium hydroxide water solution; heating in a microwave oven for reaction; drying to obtain the fluorescence gold nanoparticle. The fluorescence gold nanoparticle is about 665 micrometers in fluorescence emission peak, appears to be strong red fluorescence when being observed with a black background under natural light, can reach 7.3% in fluorescence quantum yield, is 1.8 microseconds in fluorescence lifetime and can reach more than 12 mouths in room-temperature storage stability. The preparation method is simple, quick and environment friendly, a novel approach is provided for preparation of a fluorescence gold nano-material. The fluorescence gold nanoparticle can be applied to detection of mercury ions, and limit of detection is 3x10-11mol / L.

The invention relates to a method for detecting mercury ions by nucleic acid aptamer based photoelectrochemistry. An On-Off type nucleic acid aptamer based photoelectrochemical sensor is adopted for detecting the mercury ions with different concentrations and is constructed based on quercetin copper sensitization perylene tetracarboxylic-oxidized graphene heterojunction. Based on double advantages of a quercetin copper compound as a photosensitizer and a reducing agent, the method can be used for double amplifying photoelectrical signals, and has the characteristics of being convenient, simple, super sensitive, economical, high in specificity and the like. The constructed On-Off type nucleic acid aptamer based photoelectrochemical sensor adopts current-time technology to detect the mercury ions with different concentrations in a 0.1 mol / L phosphate buffered solution at the offset electric potential of 0.2V and under optical radiation of more than 450 nm.

The invention discloses nitrogen doped graphene quantum dots as well as a preparation method and application thereof in detecting mercury ions and cysteine and belongs to the technical field of fluorescence sensation. The preparation method comprises the following steps: dissolving a carbon source compound and a nitrogen source compound into water so as to obtain a mixed water solution, and performing a hydrothermal reaction under an alkali condition, so as to obtain the nitrogen doped graphene quantum dots, wherein the carbon source compound is 1,3,6-trinitropyrene; the nitrogen source compound is tryptophan; and the alkali condition is adjusted by using sodium hydroxide. The preparation method is high in yield, and gram-grade synthesis can be achieved at one time. Due to doping of high-content nitrogen atoms, the prepared nitrogen doped graphene quantum dots have glue fluorescence and have unique mercury ion selectivity. On the basis of quenching of mercury ions upon fluorescence ofthe nitrogen doped graphene quantum dots, sensitive fluorescence detection on the mercury ions can be achieved, and the detection limit is low. As the fluorescence quenched by the mercury ions can berecovered by using the cysteine, fluorescence detection on the cysteine can be achieved with the combination of the nitrogen doped graphene quantum dots and the cysteine.

The invention discloses a mercury ion test strip and a preparation method and a testing method thereof and belongs to the field of heavy metal ion testing. The preparation method for the mercury ion test strip has the steps that the test strip comprises a hydrophilic membrane and a hydrophobic substrate; the hydrophilic membrane is dipped in a gold nanoparticle solution for a period of time, so that gold nanoparticles are attached to the hydrophilic membrane; and the hydrophilic membrane is taken out and dried; and the obtained hydrophilic membrane, attached with the gold nanoparticles, as an indicating area of the test strip, is pasted on the hydrophobic substrate so as to obtain the mercury ion test strip. The mercury ion test strip disclosed by the invention has the advantages of simple preparation method, short production period, low cost and convenient carrying and can realize field detection; and the method for detecting by using the mercury ion test strip is simple and the sensitivity of the method is high.

The invention discloses a rapid mercury detection method based on self-assembly of gold nanorods. The method of the invention mainly comprises the following steps: a nanorod self-assembly chain with substantially enlarged surface plasma resonance signals is formed through inducing the gold nanorods by phosphates; and a mercuric compound (the mercuric compound comprises inorganic mercuric ions andorganic mercury) is reduced to elemental mercury by a strong reductant sodium borohydride to change properties of surface plasmas of the gold nanorods, and the surface plasma signals are enlarged by the nanorod self-assembly chain. So the sensitive and rapid mercury detection method is established. The method of the present invention has the advantages of simple operation, good reappearance, and easy popularization.

The invention discloses a preparation method of a blotting membrane for Hg<2+> detection. The preparation method comprises the following steps: designing and synthesizing Rhodamine derivative with special developing function to Hg<2+>; and opolymerizing on a support polyvinylidene fluoride membrane by using target metal ion as a template, ethylene dimethacrylate as a crosslinking agent, azodiisobutyronitrile as a radical initiator, and the synthesized Rhodamine derivative as a function monomer to obtain the blotting membrane for mercury ion detection. The characteristic that the ionic blotting technology has high selectivity to the template ion is adopted by the invention to combine with the simplicity of a colorimetric / fluorescence chemical sensor, a colorimetric / fluorescence chemical sensing blotting membrane with high selectivity to Hg<2+> is prepared, and a fast and high-speed new scene detection method with high sensitivity and high selectivity is hopefully provided for heavy metal ions in environment and food.

The invention provides an electrochemical sensor for detecting mercury as well as a preparation method and application of the electrochemical sensor. The electrochemical sensor comprises a glassy carbon electrode, a probe P2 and a mercapto-modified probe P3 which is fixed on gold nano-particles; the surface of the detection end of the glassy carbon electrode is modified with carbon-doped graphite-like carbon nitride; the gold nano-particles deposit on the carbon-doped graphite-like carbon nitride; a mercapto-modified probe P 1 is linked to the gold nano-particles; a part of deoxyribonucleotide of the probe P2 can be in complementary pairing with a part of deoxyribonucleotide of the mercapto-modified probe P1 to form a double-chain structure; and unpaired deoxyribonucleotide in the probe P1 and probe P2 and the mercapto-modified probe P3 which is fixed on the gold nano-particles form the double-chain structure. The preparation method comprises the following steps of modifying the graphite-like carbon nitride, modifying nanogold, modifying the probe P1 and the like. The electrochemical sensor provided by the invention has strong ability of resisting heavy metal ion interference and can be applied to detecting mercury ions.

The invention discloses a detecting method for divalent mercury ions. The principle for detecting mercury ions by using the method is as follows: a single-chain DNA (Deoxyribose Nucleic Acid) molecule B modified by fluorescein contains a sequence which is complementary with a sequence of a DNA catching needle (a single-chain DNA molecule A) fixed on the surface of a probe, and also contains a T-T base pair mismatch structure which can be combined with the divalent mercury ions. During detection, the single-chain DNA molecule B modified by the fluorescein and the mercury ions are simultaneously input to the surface of an optical fiber sensor probe for evanescent waves, and the divalent mercury ions are combined with the single-chain DNA molecule B modified by the fluorescein by the DNA catching needle in a competition way. The more the concentration of the divalent mercury ions is, the fewer the single-chain DNA molecule B modified by the fluorescein and combined with the DNA catching needle on the optical fiber sensor probe for evanescent waves is, so that a fluorescence intensity signal detected by an optical detecting platform for evanescent waves is weaker. An effective means is provided for the rapid on-site and real-time detection of heavy metal mercury ions in the fields of foods, medicines and environments.

The invention discloses a fluorescent probe made of rhodamine B, diethylenetriamine and PITC (phenyl isothiocyanate) as well as a preparation method and an application thereof. Specifically, rhodamine B and diethylenetriamine react firstly to form a spiro amide structure with a stable property, a nitrogen bridging fragment is introduced, and the mixture then reacts with PITC so as to introduce a sulfur atom. By means of sulphophile affinity of mercury ions, each molecule of a novel rhodamine B-phenylthiourea derivative (namely, mercury ion fluorescent probe RDTU) obtained through the scheme can be combined with two mercury ions, so that the fluorescent probe has higher selectivity and detection sensitivity. The molecules of the fluorescent probe can perform detection in a neutral buffer solution especially in water phase. Additionally, the molecules of the fluorescent probe can detect the mercury ions from mercury chloride, and has better practicability.

The invention provides a preparation method of a red fluorescent silver nanocluster. Egg white is taken as a template and a protective agent; sodium borohydride is taken as a reducing agent; and "one kettle way" is utilized to prepare the silver nanocluster with uniform size, good stability, and red fluorescence. The method is simple to operate, relatively low in cost, and excellent in repeatability, and the raw materials are widely available. The prepared red fluorescent silver nanocluster is good in water solubility and strong in stability, and is applicable for testing mercury ions in a water system.

The present invention relates to a fast and highly selective mercury ion fluorescent probe. The mercury ion fluorescent probe is a sulfocarbonate compound, and the structural formula is as shown in the specification. The mercury ion fluorescent probe can achieve at least one of the following technical effects: highly selective identification of mercury ions; rapid response to the mercury ions; large Stokes shift; stable nature, long term preservation and use; and strong anti-interference ability.

The invention discloses a novel water-soluble polymer capable of selectively recognizing mercury ion; the structural formula of the novel water-soluble polymer is as shown in the specification, wherein R1 is hydrogen or alkyl; R2 is hydrogen, alkyl or hydroxyalkyl; the molecular weight is 1.6*10<4> to 5.5*10<6> g / mol; and the ratio of m to n is (100-200):1. The invention further discloses a synthetic method of the polymer. The novel water-soluble polymer is prepared by copolymerizing a tetraphenyl porphyrin monomer AOTPP and an acrylamide monomer through radical polymerization reaction. The invention further discloses application of the polymer. The polymer is capable of highly selectively detecting mercury ion in aqueous solution through a common ultraviolet-visible absorptionspectromtry and a steady-state fluorescence emission spectrometry.

The invention provides a biosensor for detection of mercury ions based on aptamer. An electrode is modified by an HAP2 layer and an HAP1 and probe1 layer in order. The invention also provides a preparation method of the biosensor. The biosensor provided by the invention has stable performance and good electrode repeatability, thus being suitable for mercury ion detection in food safety and biosensor industrialization practical application.

The present disclosure provides methods, recombinant DNA molecules, recombinant host cells containing the DNA molecules, and transgenic plant cells, plant tissue and plants which contain and express at least one phytochelatin biosynthetic coding sequence under the regulatory control of the strong ACT2 constitutive promoter or the light-inducible SRS1 promoter and / or a plant-expressible arsenate reductase coding sequence. Optionally the plant expressing the at least one phytochelatin biosynthetic enzyme coding sequence can also express a mercuric ion reductase coding sequence. The transgenic plants are tolerant of heavy metal ions, e.g., cadmium, arsenate and / or mercury, and can accumulate those ions from a contaminated environment, to thus, effect phytoremediation of a contaminated soil or water environment which contains mercury, cadmium and / or arsenate ions.