Preparation method and application of modified glassy carbon electrode

A glassy carbon electrode and working electrode technology, applied in the field of electrochemiluminescence, can solve the problems of unsatisfactory detection of trace mercury, narrow linear response range, high analysis cost, etc., and achieve high selectivity, simple and convenient operation, and increased sensitivity Effect

Active Publication Date: 2014-07-09
SHANTOU UNIV
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Problems solved by technology

[0003] At present, the traditional methods for the detection and quantitative analysis of mercury ions in aqueous solutions include: spectrophotometry, atomic absorption spectroscopy, plasma emission spectroscopy, cold atomic fluorescence spectroscopy, gas chromatography, etc. The detection principle is photoinduced electron transfer (PET), intramolecular Charge transfer (ICT) and chemical reaction systems, etc. Although these methods have high sensitivity, the equipment and analysis required are expensive, complicated and time-consuming, and cannot meet the needs of simple and rapid detection of trace mercury
The emerging biosensor method (enzyme inhibition method) has the advantages of fast, simple and low cost when detecting mercury ions, but the detection limit is high and the linear response range is narrow
[0004] Electrochemiluminescence (ECL) is the result of the joint action of chemiluminescence and electrochemistry. It refers to the phenomenon that molecules in the ground state transition to the excited state after obtaining energy through participating in the electrochemical reaction, and then emit light when the excited state returns to the ground state. It has a detection device Simple, high sensitivity, controllable degree of reaction, etc., but current electrochemical sensors often lack selectivity in detecting mercury ions

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  • Preparation method and application of modified glassy carbon electrode
  • Preparation method and application of modified glassy carbon electrode
  • Preparation method and application of modified glassy carbon electrode

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Embodiment 1

[0038] The invention uses an electrochemical workstation and an MPI-B multi-parameter chemiluminescence analysis and testing system.

[0039] 1. Glassy carbon electrode pretreatment: Glassy carbon electrode is treated with 0.05μm α-A1 2 o 3 After polishing, the polishing powder was rinsed with deionized water, and ultrasonicated in deionized water and ethanol for 10 min, respectively. Using a three-electrode system for detection, the working electrode is a glassy carbon electrode, the counter electrode is a platinum wire electrode, and the reference electrode is an Ag / AgCl electrode. In 0.5M sulfuric acid, set the voltage at -0.2 to 1.6V to cycle the glassy carbon electrode. Voltammetric scanning, after the detection, rinse the electrode with deionized water, dry the surface of the electrode, and set aside.

[0040] 2. Preparation of modified glassy carbon electrode:

[0041] 1) Put the pretreated electrode into 0.1mMRu(bpy) 3 2+ and 0.01MKNO 3 In the mixed solution, con...

Embodiment 2

[0045] 1. Glassy carbon electrode pretreatment: Glassy carbon electrode is treated with 0.3μm α-A1 2 o 3 After the polishing powder is polished, it is rinsed with deionized water, and ultrasonicated in deionized water and ethanol for 10 min respectively. Using a three-electrode system for detection, the working electrode is a glassy carbon electrode, the counter electrode is a platinum wire electrode, and the reference electrode is an Ag / AgCl electrode. In 0.5M sulfuric acid, set the voltage at -0.2 to 1.6V to cycle the glassy carbon electrode. Voltammetric scanning, after the detection, rinse the electrode with deionized water, dry the surface of the electrode, and set aside.

[0046] 2. Preparation of modified glassy carbon electrode:

[0047] 1) Put the pretreated electrode into 1.0mMRu(bpy) 3 2+ and 0.1MKNO 3 In the mixed solution of the mixed solution, control the anode potential at 2.0V for 10 minutes on the electrochemical workstation, take out the electrode, wash ...

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Abstract

The invention discloses a preparation method for a Ru(bpy)3<2+>-gold nanoparticle-DNA-ferrocene functionalized graphene modified glassy carbon electrode. The method comprises the following concrete steps: polishing of a glassy carbon electrode; coating of Ru(bpy)3<2+> to prepare a Ru(bpy)3<2+> modified glassy carbon electrode; coating of gold nanoparticles to prepare a Ru(bpy)3<2+>-gold nanoparticle modified glassy carbon electrode; coating of single-stranded DNA to prepare a Ru(bpy)3<2+>-gold nanoparticle-DNA modified glassy carbon electrode, wherein the sequence of the single-stranded DNA is rich in T bases, a stable double-stranded DNA structure can be formed after bonding with Hg2+, and the 5' end of the sequence of the single-stranded DNA is connected with an SH-(CH2)6 group; and coating pf ferrocene functionalized graphene so as to prepare the Ru(bpy)3<2+>-gold nanoparticle-DNA-ferrocene functionalized graphene modified glassy carbon electrode. The modified glassy carbon electrode prepared in the invention can be used for detecting the content of mercury ions in a water sample and has good electrochemiluminescence performance, excellent stability and reproducibility and high sensitivity; and operation of the method is easy and convenient.

Description

technical field [0001] The invention relates to the field of electrochemiluminescence, in particular to a Ru(bpy) 3 2+ - Preparation method and application of gold nanoparticles-DNA-ferrocene functionalized graphene-modified glassy carbon electrode. Background technique [0002] Mercury is a toxic metal element widely distributed in the environment, and it is one of the heavy metals commonly used in industries such as batteries and mining. Mercury can invade the human body through different channels such as the respiratory tract, skin or digestive tract. The food chain has a strong ability to accumulate mercury. The main symptoms. Therefore, the qualitative detection and quantitative analysis of mercury ions are of great significance to life, environmental and medical sciences, as well as industrial and agricultural production. [0003] At present, the traditional methods for the detection and quantitative analysis of mercury ions in aqueous solutions include: spectropho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N21/76
Inventor 高文华卓邦荣陈耀文林月娟鲁福身黄响
Owner SHANTOU UNIV
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