Protein glycosylation detection kit and detection method, and application
A detection kit and protein sugar technology, which are applied in the field of protein glycosylation detection kits to achieve the effects of high stability and improved stability
Active Publication Date: 2019-02-05
FUZHOU UNIV
View PDF7 Cites 6 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
But so far, the application of HCR amplification strategy in the detection of specific protein glycosylation in living cells / in vivo is still blank
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0069] Preparation and application of PTK7 protein-specific glycosylation probes: ASI probes and corresponding GCP, H1 and H2 probes were designed using the PTK7-targeting nucleic acid aptamer sgc8. The corresponding probe sequences are shown in Table 1.
Embodiment 2
[0075] EpCAM protein was used as the target protein, and HT29 cells highly expressing EpCAM were used as the research object. The corresponding probe preparation and labeling were the same as in Example 1, and the corresponding probe sequences were shown in Table 2.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more PUM
Login to view more Abstract
The invention discloses a protein glycosylation detection kit and a detection method, and an application. Specific protein targeting, an HCR-based non-enzymatic amplification strategy, and the proteinglycosylation detection kit and detection method for detecting and imaging specific protein glycosylation on a cell membrane surface in living cells and living bodies by using the HCR-based non-enzymatic amplification strategy, and detection applications in various application occasions such as an in vitro application occasion and an in vivo application occasion are achieved by using technical means such as modification of azido glycosyl on the surface of a protein by a glycosyl-labelled nucleic acid sequence, introduction of a protein probe nucleic acid sequence, and introduction of a hybridization chain reaction reagent.
Description
technical field [0001] The invention belongs to the field of biological detection, in particular to a protein glycosylation detection kit, detection method and application. Background technique [0002] Protein glycosylation is a complex post-translational modification of proteins widely present in most eukaryotic cells. More than 70% of proteins in organisms are glycoproteins. They participate in many important physiological processes, including regulating protein Folding, adhesion between cells, transport and clearance of intracellular molecules, activation of receptors, transmission of signals and endocytosis of biomacromolecules. In previous studies, more attention was paid to the overall protein glycosylation level, while less attention was paid to protein-specific glycosylation modifications. At present, more and more studies have shown that in biological processes, specific glycosylation on a specific protein is more biologically meaningful than overall glycosylation...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more Application Information
Patent Timeline
Login to view more Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68C12Q1/682
CPCG01N33/68C12Q1/682C12Q2525/301C12Q2563/107
Inventor 李娟李婧影刘淑雅杨黄浩
Owner FUZHOU UNIV
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Try Eureka
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap