Protein glycosylation detection kit and detection method, and application
A detection kit and protein sugar technology, which are applied in the field of protein glycosylation detection kits to achieve the effects of high stability and improved stability
Active Publication Date: 2019-02-05
FUZHOU UNIV
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Problems solved by technology
But so far, the application of HCR amplification strategy in the detection of specific protein glycosylation in living cells / in vivo is still blank
Method used
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Embodiment 1
[0069] Preparation and application of PTK7 protein-specific glycosylation probes: ASI probes and corresponding GCP, H1 and H2 probes were designed using the PTK7-targeting nucleic acid aptamer sgc8. The corresponding probe sequences are shown in Table 1.
Embodiment 2
[0075] EpCAM protein was used as the target protein, and HT29 cells highly expressing EpCAM were used as the research object. The corresponding probe preparation and labeling were the same as in Example 1, and the corresponding probe sequences were shown in Table 2.
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The invention discloses a protein glycosylation detection kit and a detection method, and an application. Specific protein targeting, an HCR-based non-enzymatic amplification strategy, and the proteinglycosylation detection kit and detection method for detecting and imaging specific protein glycosylation on a cell membrane surface in living cells and living bodies by using the HCR-based non-enzymatic amplification strategy, and detection applications in various application occasions such as an in vitro application occasion and an in vivo application occasion are achieved by using technical means such as modification of azido glycosyl on the surface of a protein by a glycosyl-labelled nucleic acid sequence, introduction of a protein probe nucleic acid sequence, and introduction of a hybridization chain reaction reagent.
Description
technical field [0001] The invention belongs to the field of biological detection, in particular to a protein glycosylation detection kit, detection method and application. Background technique [0002] Protein glycosylation is a complex post-translational modification of proteins widely present in most eukaryotic cells. More than 70% of proteins in organisms are glycoproteins. They participate in many important physiological processes, including regulating protein Folding, adhesion between cells, transport and clearance of intracellular molecules, activation of receptors, transmission of signals and endocytosis of biomacromolecules. In previous studies, more attention was paid to the overall protein glycosylation level, while less attention was paid to protein-specific glycosylation modifications. At present, more and more studies have shown that in biological processes, specific glycosylation on a specific protein is more biologically meaningful than overall glycosylation...
Claims
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IPC IPC(8): G01N33/68C12Q1/682
CPCG01N33/68C12Q1/682C12Q2525/301C12Q2563/107
Inventor 李娟李婧影刘淑雅杨黄浩
Owner FUZHOU UNIV
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