A protein glycosylation detection kit, detection method and application

Abstract

The invention discloses a protein glycosylation detection kit, a detection method and its application, and uses glycosyl tag nucleic acid sequences to modify azide glycosyl groups on the surface of proteins, introduce protein probe nucleic acid sequences, introduce hybridization chain reaction reagents and other technologies Means to achieve specific protein targeting, HCR-based non-enzyme amplification strategy, use it in living cells, specific protein glycosylation detection on cell membrane surface in vivo, imaging protein glycosylation detection kits, detection methods, and in vivo Detection applications in various applications such as in vitro and in vivo.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to a protein glycosylation detection kit, detection method and application. Background technique [0002] Protein glycosylation is a complex post-translational modification of proteins widely present in most eukaryotic cells. More than 70% of proteins in organisms are glycoproteins. They participate in many important physiological processes, including regulating protein Folding, adhesion between cells, transport and clearance of intracellular molecules, activation of receptors, transmission of signals and endocytosis of biomacromolecules. In previous studies, more attention was paid to the overall protein glycosylation level, while less attention was paid to protein-specific glycosylation modifications. At present, more and more studies have shown that in biological processes, specific glycosylation on a specific protein is more biologically meaningful than overall glycosylation...

AI Technical Summary

Problems solved by technology

But so far, the application of HCR amplification strategy in the detection of specific protein glycosylation in living cells / in vivo is still blank

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