11results about How to "Realize visual detection" patented technology

The invention relates to a finger-pressing type nucleic acid detection micro-fluidic chip, which comprises a bottom layer, a flow channel layer, a finger-pressing layer and a thin film layer, the flow channel layer comprises an LAMP reaction chamber, a CRISPR reaction chamber, a water storage chamber, a Tesla valve, a finger-pressing chamber and a test paper reaction chamber, the LAMP reaction chamber comprises a nucleic acid extraction-free reaction system based on LAMP, the CRISPR reaction chamber comprises a CRISPR-based cas12b reaction system, and the test paper reaction chamber comprises a Tesla valve, a finger-pressing chamber and a test paper reaction system. The water storage chamber is mainly used for diluting a mixed system, and the Tesla valve is used for preventing crosstalk of the two reaction systems; the finger pressing layer comprises an LAMP reaction chamber button and a CRISPR reaction chamber button, when the LAMP reaction chamber button is pressed, liquid in the LAMP reaction chamber is pumped into the CRISPR reaction chamber, when the two buttons are pressed at the same time, a reaction mixing system is pumped into the water storage chamber to be mixed, and then the reaction mixing system is further pumped into the test paper reaction chamber. The chip can be widely applied to the field of rapid diagnosis of nucleic acid molecules, the detection cost is reduced, manual operation is simplified, and the detection efficiency is improved.

The present application relates to a kind of termite inner nest structure distribution visualization nondestructive detection equipment and positioning method, the nondestructive detection device includes signal transmitter, signal array receiver and acquisition host, the acquisition host is connected with signal transmitter and signal array receiver, acquisition host is equipped with signal transmission module, signal acquisition module, signal processing module and signal visualization module.The present application uses the detection method of active source diffraction secondary sound source, carries out the detection positioning of ant nest structure, accurately tracks and locates the distribution of ant road by arranging signal transmitter and array type arrangement sound source sensor, accurately detects the termite nest structure in dam interior, more effectively eliminates potential leakage hidden danger caused by ant nest.

This invention belongs to the field of nucleic acid aptamer technology. This invention provides a high-affinity and specific phalloidin nucleic acid aptamer and its applications. The base sequence of the high-affinity and specific phalloidin nucleic acid aptamer is shown in SEQ.ID.NO.1. The nucleic acid aptamer obtained by this invention exhibits strong specificity for phalloidin; after detection by graphene oxide fluorescence quenching, the measured fluorescence intensity reaches 867.6 (au), while its effects on other toxins and blank controls are not significant. Furthermore, it has high affinity for phalloidin, with a Kd value of 240 nM (0.203 ng / Kg). After 5' end FAM fluorescent labeling, the nucleic acid aptamer obtained by this invention can achieve visual detection of phalloidin under ultraviolet transmission irradiation.

This invention discloses a combined ultrasound probe device, comprising a control unit, a motion unit, a pressure detection unit, a temperature detection unit, a camera unit, a combined ultrasound probe unit, a water system unit, an external control unit, and a transducer unit. The control unit is used for control and data interconnection; the motion unit is used for movement of the probe unit and transducer unit; the pressure detection unit is used to detect the contact pressure between the water bag and the human body; the temperature detection unit is used to detect the temperature of the medium water; the camera unit is used to acquire image data from the water bag; the combined ultrasound probe unit is used to acquire image data from the target area; the water system unit is used for water intake and drainage of the water bag; the external control unit communicates with the control unit for data exchange and information interconnection; and the transducer unit is used for ultrasound emission and transmission of transducer data parameters to the external control unit. This invention can find the optimal target position and precisely control the treatment energy while maintaining a comfortable treatment position for the patient.

The application belongs to the field of detection, and specifically discloses a fluorescent probe, a preparation method and application thereof; the fluorescent probe comprises a compound shown in formula (I). 2+ The fluorescent probe has high sensitivity and selectivity, is not interfered by other metal ions, has a short detection time, can be detected within 30 min, can be detected completely within 30 s at most, can realize visual detection when applied to a test strip, and can be applied to the detection of Hg 2+ in environmental samples, such as actual water samples and soil samples.

This invention belongs to the field of immunoassay detection and discloses a ZrMPNs-NlucmAb bioluminescent signal probe and its application. The probe consists of a ZrMPNs signal carrier and its electrostatically adsorbed bioluminescent enzyme-labeled monoclonal antibody NlucmAb. This invention utilizes the ZrMPNs-NlucmAb probe to construct a bioluminescent test strip (BLFA), which requires no external light source and achieves a high luminescent signal in a furazine (FZ)-Nluc system, thus enabling sensitive detection of OTA. The limit of detection (LOD) of BLFA is 0.02 ng / mL, which is 9 times more sensitive than traditional gold nanoparticle-based LFAs. The cross-reactivity rates of this BLFA to OTB and OTC are 122.2% and 111.1%, respectively, and it does not recognize other common fungal toxins, exhibiting good specificity. Its application in the detection of OTA in cereals shows great promise.

The application relates to a preparation method of cobalt-modified polyheptazine imide and application thereof in rhodamine B photosensitization detection of paracetamol, and relates to the technical field of visual super-sensitive detection of colorless paracetamol. Method: the cobalt-modified polyheptazine imide is used to detect paracetamol in the following modes: mode one, rhodamine B-cobalt-modified polyheptazine imide is uniformly mixed with a paracetamol solution to obtain a to-be-detected suspension; the suspension is stirred and adsorbed in a Porphyrin multi-channel photocatalytic reactor under the condition of no light, a filter membrane is used for filtration after 532nm visible light is turned on, and ultraviolet-visible spectrophotometry is used for detection; mode two, the to-be-detected suspension is injected into a liquid-core optical fiber probe and is packaged, then a 532nm single-wavelength light source is turned on, and light irradiation is performed for 3-5min. The application can obtain the preparation method of cobalt-modified polyheptazine imide and application thereof in rhodamine B photosensitization detection of paracetamol.

The application relates to the technical field of nucleic acid detection in biochemistry, and provides a novel coronavirus nucleic acid instant detection system based on CRISPR / Cas12a and a method thereof. The system comprises specific constant-temperature amplification primers N-F / R for a novel coronavirus N gene, and a CRISPR / Cas12a reaction system, wherein the CRISPR / Cas12a reaction system comprises specific crRNA for the N gene, CRISPR / Cas12a protein and a single-stranded DNA reporting system. The lateral flow chromatographic test strip detection method of the constant-temperature amplification CRISPR / Cas12a has high specificity and sensitivity, and visual detection can be realized in about 35 minutes; the freeze-dried reagent simplifies the operation process, accelerates the experiment process, gets rid of cold-chain transportation, saves transportation cost, and can realize reaction under the condition of constant temperature of 37 DEG C; the result is simple to judge and can be observed by naked eyes, and is suitable for family nucleic acid self-test.

This invention relates to boron-nitrogen co-doped fluorescent carbon quantum dots, their preparation method, and their application in the fabrication of chromium / copper ion sensors. It belongs to the field of carbon quantum dot technology and includes the following steps: mixing o-phenylenediamine hydrochloride and boric acid to obtain a mixed solution; adding water to the mixed solution and ultrasonically dissolving it to obtain a mixed solution; treating the mixed solution at high temperature to obtain a boron-nitrogen co-doped carbon material solution; filtering the carbon material solution through a 0.22 μm microporous membrane to obtain a boron-nitrogen co-doped carbon quantum dot aqueous solution, and then dialyzing it using a dialysis bag for 36-48 h; freeze-drying the carbon quantum dot aqueous solution to obtain boron-nitrogen co-doped fluorescent carbon quantum dots. This invention achieves pH-controlled chromium / copper ion sensors using a single quantum dot material and a one-step synthesis process. 6+ / Cu 2+ The dual-channel high-sensitivity detection overcomes the technical barriers of limited probe selectivity, complex processes, and limited functionality in existing probes.

This invention discloses the application of a pyrene-containing fluorescent probe in the detection of straight-chain fatty alcohols, belonging to the field of lyochromic organic small molecule fluorescent probe technology. The structural formula of the pyrene-containing fluorescent probe is shown in formula (1): where R is hydrogen or iodine. This pyrene-containing fluorescent probe can produce ratiometric dual fluorescence emission dependent on the carbon chain length of straight-chain fatty alcohols, and has ratiometric response capability to structurally similar straight-chain fatty alcohol homologues such as methanol, ethanol, n-propanol, n-butanol, n-pentanol, and n-hexanol, and can be used in the detection of straight-chain fatty alcohols.

The invention belongs to the technical field of light-emitting functional materials and temperature sensing, and particularly relates to a fluorescent temperature sensing material based on a 3d / 4f complex and a preparation method and application of the fluorescent temperature sensing material. The fluorescent temperature sensing material based on the 3d / 4f complex provided by the invention is prepared by taking a Schiff base ligand H2L, samarium nitrate hexahydrate and anhydrous zinc acetate as reaction raw materials through an evaporative crystallization method; the structural formula of the Schiff base ligand H2L is shown in the specification; the molecular structural formula of the fluorescent temperature sensing material based on the 3d / 4f complex is [ZnLSm (CH3OH) (NO3) 2]. The fluorescent temperature sensing material based on the 3d / 4f complex provided by the invention can keep stable in a wide temperature range, has a linear fluorescence intensity ratio, can realize quantitative detection or visual detection of temperature, is high in detection sensitivity, can provide a technical basis and a research and development direction for development of the temperature sensing material, and has a wide application prospect. And the method has excellent application value and prospect.