4results about How to "Realize visual detection" patented technology

This invention belongs to the field of nucleic acid aptamer technology. This invention provides a high-affinity and specific phalloidin nucleic acid aptamer and its applications. The base sequence of the high-affinity and specific phalloidin nucleic acid aptamer is shown in SEQ.ID.NO.1. The nucleic acid aptamer obtained by this invention exhibits strong specificity for phalloidin; after detection by graphene oxide fluorescence quenching, the measured fluorescence intensity reaches 867.6 (au), while its effects on other toxins and blank controls are not significant. Furthermore, it has high affinity for phalloidin, with a Kd value of 240 nM (0.203 ng / Kg). After 5' end FAM fluorescent labeling, the nucleic acid aptamer obtained by this invention can achieve visual detection of phalloidin under ultraviolet transmission irradiation.

This invention discloses a combined ultrasound probe device, comprising a control unit, a motion unit, a pressure detection unit, a temperature detection unit, a camera unit, a combined ultrasound probe unit, a water system unit, an external control unit, and a transducer unit. The control unit is used for control and data interconnection; the motion unit is used for movement of the probe unit and transducer unit; the pressure detection unit is used to detect the contact pressure between the water bag and the human body; the temperature detection unit is used to detect the temperature of the medium water; the camera unit is used to acquire image data from the water bag; the combined ultrasound probe unit is used to acquire image data from the target area; the water system unit is used for water intake and drainage of the water bag; the external control unit communicates with the control unit for data exchange and information interconnection; and the transducer unit is used for ultrasound emission and transmission of transducer data parameters to the external control unit. This invention can find the optimal target position and precisely control the treatment energy while maintaining a comfortable treatment position for the patient.

The application belongs to the field of detection, and specifically discloses a fluorescent probe, a preparation method and application thereof; the fluorescent probe comprises a compound shown in formula (I). 2+ The fluorescent probe has high sensitivity and selectivity, is not interfered by other metal ions, has a short detection time, can be detected within 30 min, can be detected completely within 30 s at most, can realize visual detection when applied to a test strip, and can be applied to the detection of Hg 2+ in environmental samples, such as actual water samples and soil samples.

This invention belongs to the field of immunoassay detection and discloses a ZrMPNs-NlucmAb bioluminescent signal probe and its application. The probe consists of a ZrMPNs signal carrier and its electrostatically adsorbed bioluminescent enzyme-labeled monoclonal antibody NlucmAb. This invention utilizes the ZrMPNs-NlucmAb probe to construct a bioluminescent test strip (BLFA), which requires no external light source and achieves a high luminescent signal in a furazine (FZ)-Nluc system, thus enabling sensitive detection of OTA. The limit of detection (LOD) of BLFA is 0.02 ng / mL, which is 9 times more sensitive than traditional gold nanoparticle-based LFAs. The cross-reactivity rates of this BLFA to OTB and OTC are 122.2% and 111.1%, respectively, and it does not recognize other common fungal toxins, exhibiting good specificity. Its application in the detection of OTA in cereals shows great promise.