237results about How to "Realize visual detection" patented technology

The invention discloses a spatially-separated pump-probe transient absorption spectrograph and a realization method. The realization method is characterized by generating a light source through a femtosecond light source system; realizing the beam splitting of pump light and probe light through a beam splitter; realizing the different time delay of the probe light through a time delay line; realizing the two-dimensional rotation and the calibration of the probe light within a horizontal plane and a vertical plane through a sweep reflector group; calibrating which means guaranteeing the incidence of the rotated probe light into a aperture within the front section of an objective lens; finally, obtaining a two-dimensional image formed on a sample under the combined action of the probe light and the pump light by a data collection system. According to the spatially-separated pump-probe transient absorption spectrograph and the realization method, the extremely high spatial discrimination can be realized, and moreover, the visual probe of carriers, excitors or plasmons can be realized.

The invention relates to a dual-emission ratio-type quantum dot fluorescence probe for visual detection of aspirin, a preparation method and an application thereof and belongs to the technical field of preparation of material and detection of content of medicines. The preparation method of the probe includes following steps: preparing a precursor NaHTe solution from sodium borohydride, tellurium powder and water under an ultrasonic environment; adding the precursor to an aqueous solution of CdCl2.2.5H2O in the presence of thioglycollic acid; carrying out a reflux reaction with a nitrogen protecting condition to obtain required green fluorescence quantum dot and red fluorescence quantum dot; by means of a sol-gel method, wrapping the red fluorescence quantum dot with silicon spheres with amino group being connected; dispersing a green fluorescence quantum dot solution and the red fluorescence quantum dot wrapped with the silicon spheres in an MES buffer solution with addition of an EDC/NHS solution; and carrying out a reaction at room temperature in a dark place to obtain the dual-emission ratio-type quantum dot fluorescence probe. The dual-emission ratio-type quantum dot fluorescence probe is used in detection of the content of the aspirin through fluorescence quantitation and visualized analysis. The quantum dot fluorescence probe is quite good in optical performance and stability and has a capability of visualizedly detecting the aspirin.

The invention relates to the technical field of preparation of bio-functional materials, in particular to dual-emission fluorescent molecularly imprinted polymer nanoparticles and a preparation methodand application thereof. carbon quantum dots are coated with silica nanospheres to form a core of a ratio fluorescence probe; red cadmium telluride quantum dots are used as response signals, acrylamide and 4-vinylbenzeneboronic acid are used as bifunctional monomers, N,N-methylene bisacrylamide is used as a crosslinker, dopamine is used as templating molecules, dual-emission fluorescent molecularly imprinted polymer nanoparticles having specific recognition sites for dopamine are synthesized in an alcohol phase under initiating of azodiisobutyronitrile, and fluorescent detection test paper with visualizing effect is prepared then by means of impregnating so as to provide visual detection for DA (dopamine); the fluorescent detection test paper is applied to the detection of DA in a human serum sample, and the results prove that the fluorescent detection test paper prepared herein is suitable for half-quantitative detection of DA in actual complex samples.

The invention relates to a method for visualized detection of organophosphorus pesticide residue by a doped quantum dot ratio fluorescence technique. Being a standard curve method, the method includes preparation and quenching of a double-fluorescence emission quantum dot, establishment of a standard curve and detection of organophosphorus pesticide residue. The method is characterized in that: the standard curve is determined under ultraviolet irradiation and is a curve about the corresponding relationship of a fluorescence ratio and a sample concentration, i.e. the standard curve is established by adding sample solutions with gradient concentrations into a quenched doped quantum dot probe dispersion fluid with a concentration of 30-40mcg/ml in order under ultraviolet irradiation, and determining fluorescence ratios successively. For the quenched doped quantum dot, a quenching agent is employed to quench the double-fluorescence of the doped quantum dot probe dispersion fluid to fluorescence intensity that no longer reduces. The method provided in the invention realizes sensitive and high selectivity detection of organophosphorus pesticide residue.

The invention relates to a potassium ion concentration detection method. The characteristic for forming the conversion of a G-four chain deoxyribonucleic acid (DNA) structure by controlling potassium ions and the characteristic for recognizing the conversion of the G-four structure through cyanine dye supramolecular aggregate are utilized, a sample to be tested is placed into DNA and cyanine dye mixed solution capable of forming the G-four chain, and the concentration range of the potassium ions in the sample can be semiquantitatively judged according to the color of the solution. Due to adopting the method, the visible detection of the potassium ion concentration can be realized, and detection test paper can be developed. A reagent is simple in ingredients, the reaction process is simple, no special or additional instrument is needed, the detection cost is low, and the method is convenient for being popularized to use in the industry.

The invention belongs to the field of molecular biology and relates to cascade intrusion signal amplification reaction combined nanogold-oligonucleotide probe visual detection. The cascade intrusion signal amplification reaction combined nanogold-oligonucleotide probe visual nucleic acid detection method provided by the invention comprises the following steps of: 1) first-step intrusion signal amplification reaction, during which accumulation of flap fragments is formed; 2) flap fragment circulation phase, during which complementary hybridization of the flap fragments and hairpin probes is performed to form a flap fragment-hairpin probe special structure, and the hairpin probes are cut after the structure is recognized by enzymes; and 3) nanogold-oligonucleotide probe detection phase, during which results of the cascade intrusion signal amplification reaction are visually detected by the utilization of two different oligonucleotide modified nanogold probes. By the utilization of the detection method provided by the invention, visual detection of a nucleic acid target sequence can be carried out. In addition, as the intrusion signal amplification reaction has high specificity, differentiated detection of the nucleic acid sequences with single-base differences near intrusion sites can be realized.

The invention relates to a potassium ion concentration detection method. According to the method, characteristics comprising that potassium ion regulation allows G-quadruplex DNA structure transformation to be formed and cyanine dye supermolecular aggregates identify the G-quadruplex DNA structure transformation are utilized, a sample to be detected is added to a mixed solution of G-quadruplex DNA and the cyanine dye, the absorbance value at 560-590nm, 500-540nm or 610-670nm or the fluorescence intensity value at 580-640nm is determined, and the corresponding potassium ion concentration value can be obtained through finding the value corresponding to the absorbance value or the fluorescence intensity value on a standard curve. The method has a high specificity, so the method is not affected by sodium ions in the sample; and reagent components is simple, and the reaction process is simple, so errors generated by operations can be effectively reduced, and the test accuracy is high. The method can be rapidly realized through a common ultraviolet-visible absorption detector, a spectrophotometer or a fluorescent spectrometer without special or extra instruments, so the detection cost is low, thereby the popularization and the application of the method in industries are convenient.

The invention discloses a preparation method and a Cu<2+> detection application of a rare earth ratiometric fluorescent probe, and belongs to the technical field of environmental detection. The preparation method of the luminol-Tb-GMP fluorescent probe is established through the self-polymerization coordination effect among luminol, GMP and Tb<3+> by using luminol and guanosine monophosphate (GMP)as dual ligands and using a rare earth ion Tb<3+> as a luminescent center ion. The luminol-Tb-GMP fluorescent probe combines the dual fluorescent signals of luminol and Tb<3+>, and simultaneously emits dual fluorescent signals of the luminol and Tb<3+> under the same excitation wavelength. When Cu<2+> exists, the luminol and GMP have a strong coordination effect on the Cu<2+> in order to preventelectrons from being transferred from the amino group of the GMP to Tb<3+>, so fluorescence quenching of the Tb<3+> is caused, and the fluorescence intensity of the luminol is unchanged, thereby a Cu<2+> detection method based on the fluorescence signal ratio of the luminol and Tb<3+> is established. Additionally, the green fluorescence of the Tb<3+> gradually weakens and the blue fluorescence ofthe luminol gradually appears with the increase of the Cu<2+> concentration, so the visual detection of the Cu<2+> can be achieved. The luminol-Tb-GMP fluorescent probe also can be applied to the sensitive detection of the Cu<2+> in an environmental water samples and a biological sample.

The invention relates to a potassium ion concentration detection kit and a system thereof. The kit and the system, which utilize characteristics comprising that potassium ion regulation allows G-quadruplex DNA structure transformation to be formed and cyanine dye supermolecular aggregates identify the G-quadruplex DNA structure transformation, have high specificities, and are not affected by sodium ions in a sample. Reagent components are simple and the reaction process is simple, so errors generated by operations can be effectively reduced, and the test accuracy is high. The kit and the system of the invention allow rapid detection to be realized without special or extra apparatuses, and the detection cost is low, so the popularization and the application of the kit and the system are convenient.

The invention belongs to the fields of chemistry, materials and food safety, and relates to a molecular imprinting ratio fluorescent sensor, a preparation method and application thereof. The preparation method of the molecular imprinting ratio fluorescent sensor includes the steps that molecular imprinting is carried out through sol-gel polymerization, single-component dual-fluorescence emission cadmium telluride/8-hydroxyquinoline zinc nanoparticles provide fluorescence signals, holes after eluting bright blue are recognition sites, a mesoporous structure is formed after hexadecyl trimethyl ammonium bromide is eluted, and bright blue imprinted microspheres with the mesoporous structure are obtained, that is, the molecular imprinting ratio fluorescent sensor based on the single-component dual-fluorescence emission nanoparticles. According to the molecular imprinting ratio fluorescent sensor, preparation and application thereof, the obtained sensor prepared by the preparation method candetect bright blue with high selectivity and sensitivity, and provides color evolution and self-correction functions; and in addition, by effectively adjusting the emission wavelength of the sensor,the molecular imprinting ratio fluorescent sensor can be more widely used in detection of various colored substances.

The invention discloses a noble metal nanoparticle based visual detection method and an application, and relates to the field of immunity detection and analysis. The method combines the high catalytic performance of an enzyme and the high extinction coefficient of a noble metal nanoparticle, the visual detection concentration of the enzyme is greatly reduced, meanwhile, the small change of the concentration of the enzyme is conveniently distinguished through the color of an obtained nano-gel, and the concentration of the enzyme is semi-quantitatively detected directly through naked eyes within a certain concentration range of the enzyme. The method is combined with a traditional enzyme linked immunosorbent assay (ELISA), and various tumor makers, bacteria and viruses are visually detected with high sensitivity. The method is based on the fact that the direct in-situ growth of the nanoparticle leads to the remarkable color change from colorless solution to colored solution, no complicated probe modification process or no precise instrument and equipment is needed, therefore, the method is simple and is low in energy consumption, and the method has a very high practical value.

The invention discloses an electrochemiluminescence immunosensor based on CdZnTeS quantum dots. The electrochemiluminescence immunosensor comprises CdZnTeS-Ab2, Fe3O4@SiO2-Ab1 with closed non-specificbinding sites, and antigens respectively connected with the CdZnTeS-Ab2 and the Fe3O4@SiO2-Ab1 by mutual action between antigens and antibodies; the CdZnTeS-Ab2 is secondary antibodies marked by CdZnTeS quantum dots; the Fe3O4@SiO2-Ab1 is Fe3O4@SiO2 with the surface being modified with primary antibodies. The invention also provides a preparation method and application of the electrochemiluminescence immunosensor. The electrochemiluminescence immunosensor, the preparation method and the application disclosed by the invention have the beneficial effects that the electrochemiluminescence immunosensor is established on the basis of mutual action between the antigens and the antibodies, so that the specificity of detection is greatly improved, the detection speed is fast, the sensitivity is high, the specificity is good, the detection limit is low, the detectable linear range is wide, the operation is simple and visual detection can be realized.

The invention discloses a preparation method of a visual detection chip for multiple pesticide residues based on a membrane material. The preparation method comprises the steps of spotting a detection point and quality-control points on a carrier to form a point array, the carrier being a nitrocellulose membrane; washing with a cleaning solution; drying in the air; sealing uncombined regions by using bovine serum albumin; washing with the cleaning solution; and drying in the air to obtain the visual detection chip for the multiple pesticide residues. The quality-control points comprises a positive quality-control point and a negative quality-control point; the positive quality-control point is goat anti mouse IgG; the negative quality-control point is 0.01 M phosphate buffer solution PBS (pH7.4); and the detection point is a pesticide hapten-chicken ovalbumin conjugate. The invention also provides a use method of the visual detection chip for the multiple pesticide residues. According to the invention, visualization of an enhanced gold mark color development and high throughput of a chip technology are integrated together, and an immune chip capable of determining by naked eyes and detecting a plurality of pesticide residues at the same time is prepared.

The invention discloses a nano di-cyclic aptamer probe and application thereof. The nano di-cyclic aptamer probe (G-dApR) is very stable and is not degraded by in-vivo DNA. Meanwhile, the biological activity of target cells can be effectively inhibited by combining specific recognition with a leukemia cell surface highly-expressive biomarker. The design of the probe is researched and developed based on two advanced technologies in the field of tumors, namely a ligand technology and a nanotechnology. Firstly, a conventional linear aptamer structure is transformed into a structure including a functional zone (specific) and a di-cyclic DNA zone (stable) by adopting a nucleic acid modification means, and the biological stability is greatly improved; secondly, the probe is finally established by combining a cyclic aptamer and a dendritic nano material PAMAM. The shortcoming that an aptamer is unstable is greatly overcome, an effective means is provided for leukemia detection, and high-sensitivity diagnosis and treatment integrated clinical application is achieved.

The invention provides a kit for detecting circulating nucleic acid based on a micro-fluidic chip and G-quadruplex-protoheme DNA enzyme, and a preparation method and application of the kit, and belongs to the technical field of biomolecular detection. The kit for detecting the circulating nucleic acid comprises a micro-fluidic detection chip, gold nanoparticle liquid, a first hairpin probe reagent, a second hairpin probe reagent and a light-emitting system, wherein the micro-fluidic detection chip is coated with functionalized microspheres; the functionalized microspheres are used to modify afirst probe on the surface of the microsphere through a biotin-avidin system; the surface of the gold nanoparticle liquid is modified by a second probe and a triggering probe. The reagent provided bythe invention is applied to detection on the circulating nucleic acid. The kit can solve the problem that high throughput and high sensitivity are difficult to coexist under the condition of a trace amount of samples in the technical field of micromedical analysis or the field of minimally invasive or non-invasive diagnosis.

The invention belongs to the field of analysis methods of radioactive elements, and particularly relates to a method for analyzing and detecting trace radioactive elements by a double-channel fluorescent uranyl ion probe. The probe is established on the basis of a semiconductor quantum dot-carbon quantum dot double-channel fluorescent signal with a method mainly comprising the following steps: (1)synthesizing a green fluorescent carbon quantum dot; (2) synthesizing a red fluorescent cadmium quantum dot; (3) preparing the probe. The detection principle is that uranyl ions can quench fluorescence of the red quantum dot, while the green fluorescence signal of the carbon quantum dot remains stable and unchanged, and quantitative detection of a to-be-detected sample is realized by testing thelinear relationship between concentration of the sample and the ratio of the red fluorescence signal to the green fluorescence signal. With adoption of the method, use of large-scale instruments can be avoided to a certain extent, all that is required is only one hand-held ultraviolet lamp for visual detection, operation is simple, convenient and quick, the pretreatment process is omitted, and high-sensitivity selective detection of the trace uranyl ions in a solution can be realized.

The invention discloses a construction method for disposing road cracks by using waterborne polyurethane. The method comprises the following steps: carrying out nondestructive testing on road cracks, drawing a crack distribution diagram, cutting and slotting cracks with the width of less than 3mm as required, drilling grouting holes at intervals along the trend of the cracks, installing grouting pipes, injecting water into the cracks; and then injecting a waterborne polyurethane grouting material, enabling the slurry to react with water in the cracks to generate gel to fill the cracks, sealing the grouting holes after grouting is completed, and finally performing rechecking to confirm that repairing is completed. The method has the advantages that visual detection of road cracks can be achieved, follow-up grouting repair is effectively guided, the characteristics that the waterborne polyurethane material is high in water reaction speed and short in gelation time are ingeniously utilized, roadbed and pavement cracks can be rapidly and comprehensively repaired, the material cannot generate a corrosion effect. And the road crack disease is effectively solved, and wide applicability is achieved.

The invention discloses a hydrogel microcarrier and a preparation method and application thereof.The hydrogel microcarrier takes an oil phase solution as a carrier, an aqueous phase solution containing an initiator and two or more gelatinizing materials is dispersed in the oil phase solution, and the density-coded hydrogel microcarrier is formed by changing the concentration ratio of different gelatinizing materials. The preparation method comprises the following steps: mixing two or more gelatinizing materials with different concentration ratios with an initiator to obtain a water-phase solution; dissolving a surfactant and TEMED in an oil medium to obtain an oil phase solution, wrapping a water phase solution in the oil phase solution to form hydrogel liquid beads, and curing the hydrogel liquid beads to form the density-coded hydrogel microcarrier. The hydrogel microcarrier can realize multiple detection of disease-related proteins and visual detection of tumor-derived exosomes, andhas the advantages of simple decoding, low detection cost and the like.

The invention discloses an LAMP kit for Vibrio cholera in an aquatic product. The kit comprises a reaction buffer solution, specific primers for detecting the Vibrio cholera, a BstDNA polymerase, a Vibrio cholera DNA positive template and ultrapure water; and the specific primers for detecting the Vibrio cholera comprise a pair of inner primers FIP and BIP and a pair of outer primers F3 and B3. The kit has the characteristics of strong specificity, high sensitivity, simplicity and fastness, and can be used for real-time rapid and accurate detection of Vibrio cholera of a base.

The invention relates to a method for detecting nucleic acid based on exponential hairpin assembly and colorimetry. The method comprises the following steps in sequence: 1) hybridizing target nucleic acid molecules and DNA (deoxyribonucleic acid) on nanogold; 2) initiating four hairpin DNAs to perform self-assembly reaction by the target nucleic acid molecules and generating large DNA polymers; 3) adding salts of divalent cations or monovalent cations into a reaction solution, and performing different degrees of aggregation on the nanogold combined with the target nucleic acid molecules, wherein the color of the nanogold changes from red to blue. According to the method, a system is simple, and the four hairpin DNAs are designed easily; the visual inspection of a target can be realized; the method has high temperature adaptability, high reaction speed, high sensitivity, high specificity and short reaction time, and can be applied to the field of clinical detection, food safety detection, environment monitoring and the like; the detection limit reaches 8.45 pM.

The invention provides a method for detecting lipase activity. The method comprises the following steps: mercaptoacetic acid methyl ester is utilized for modifying nanogold; in the presence of lipase, mercaptoacetic acid is generated by hydrolysis, and thus carboxylate radicals are exposed; intense hydrogen-bond interaction exists among carboxylate radicals, and thus the distance between nanogold is shortened to gather and blue the nanogold. On one hand, the strength of the lipase activity can be directly identified by naked eyes from the color and on the other hand, the lipase activity can be sensitively detected by an ultraviolet and visible spectrophotometer. The method can be used for detecting the lipase activity from the aspects of time, temperature, quantity and the like and the experimental phenomenon is obvious. The method has the advantages of being simple to operate without substrate emulsification, small in dosage, being beneficial to being obviously observed by naked eyes and low in cost. The method has not only good research value academically, but also wide application in production application.