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Noble metal nanoparticle based visual detection method and application

A technology of nanoparticles and detection methods, applied in biological testing, material testing products, resistance to vector-borne diseases, etc., can solve problems such as false negative signals, false positives of metal nanoparticles, and inability to apply direct detection, and achieve convenient detection methods. , the effect of low consumption scope

Active Publication Date: 2016-12-21
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the complex modification process is likely to lead to non-specific aggregation of metal nanoparticles, resulting in false positive or false negative signals.
In addition, although many methods reported so far have achieved good sensitivity, most of them are not suitable for clinical direct detection

Method used

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  • Noble metal nanoparticle based visual detection method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0038] A visual detection method based on in situ growth of silver nanoparticles 1

[0039] AA-P was dissolved in 160mM DEA buffer to make the final concentration 10mM. Then 100 μL of the above solution was added to a 96-well plate and incubated with different concentrations of ALP at 37° C. for 1 h. Then 3 μL of 1 M silver nitrate solution was added to start the redox reaction to grow silver nanoparticles in situ. Observe the color change in the well plate with naked eyes, and take pictures with a camera to obtain visual detection results, and at the same time use a microplate reader to read the absorbance value of AgNPs at 400nm (A 400nm ), the concentration of ALP and A 400nm Do a linear fit. Such as figure 2 , a) is the visual detection result diagram of the in situ growth of silver nanoparticles under different enzyme concentration conditions; b) the ultraviolet-visible absorption spectrum of the silver nanoparticle solution obtained under the different enzyme concen...

Embodiment 2

[0041] A visual detection method based on in situ growth of silver nanoparticles 2

[0042] Dissolve AA-P in 120mM DEA buffer to make the final concentration 8mM. Then 100 μL of the above solution was added to a 96-well plate and incubated with different concentrations of ALP at 37° C. for 1 h. Then 2.5 μL of 1M silver nitrate solution was added to start the redox reaction to grow silver nanoparticles in situ. Observe the color change in the well plate with naked eyes, and take pictures with a camera to obtain visual detection results, and at the same time use a microplate reader to read the absorbance value of AgNPs at 400nm (A 400nm ), the concentration of ALP and A 400nm Do a linear fit.

Embodiment 3

[0044] A visual detection method based on in situ growth of gold nanoparticles 1

[0045] AA-P was dissolved in 160mM DEA buffer to make the final concentration 10mM. Then 100 μL of the above solution was added to a 96-well plate and incubated with different concentrations of ALP at 37° C. for 1 h. Then, 3 μL of 1 M chloroauric acid solution was added to start the redox reaction to grow gold nanoparticles in situ. Observe the color change in the well plate with the naked eye, and take pictures with a camera to obtain visual detection results, and at the same time use a microplate reader to read the absorbance value of AuNPs at 530nm (A 530nm ), the concentration of ALP and A 530nm Do a linear fit.

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Abstract

The invention discloses a noble metal nanoparticle based visual detection method and an application, and relates to the field of immunity detection and analysis. The method combines the high catalytic performance of an enzyme and the high extinction coefficient of a noble metal nanoparticle, the visual detection concentration of the enzyme is greatly reduced, meanwhile, the small change of the concentration of the enzyme is conveniently distinguished through the color of an obtained nano-gel, and the concentration of the enzyme is semi-quantitatively detected directly through naked eyes within a certain concentration range of the enzyme. The method is combined with a traditional enzyme linked immunosorbent assay (ELISA), and various tumor makers, bacteria and viruses are visually detected with high sensitivity. The method is based on the fact that the direct in-situ growth of the nanoparticle leads to the remarkable color change from colorless solution to colored solution, no complicated probe modification process or no precise instrument and equipment is needed, therefore, the method is simple and is low in energy consumption, and the method has a very high practical value.

Description

technical field [0001] The invention relates to the field of immune detection and analysis, in particular to a visual detection method and application based on noble metal nanoparticles. Background technique [0002] The colorimetric method has the advantages of directly reading the signal with the naked eye and does not need to resort to other sophisticated and expensive instruments and equipment, so it has been applied in many aspects. Many methods related to colorimetry reported in the past mostly achieve the purpose of color change by controlling the aggregation and dispersion state of gold or silver nanoparticles. However, these methods often need to modify the surface of metal nanoparticles, or add some electrolytes to their solution to change the charge on the surface, so as to achieve the purpose of changing the color of the solution. However, the complex modification process is likely to lead to non-specific aggregation of metal nanoparticles, resulting in false po...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/25G01N33/53
CPCG01N21/25G01N21/31G01N33/53Y02A50/30
Inventor 刘定斌轩志宏
Owner NANKAI UNIV
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