11 results about "Microplate Reader" patented technology

The present application relates to the technical field of enzyme label instrument, specifically, it is a kind of portable biological enzyme label instrument and its using method, including enzyme label instrument body and the detection cavity being arranged in the inside of enzyme label instrument body, first conveying belt and second conveying belt for carrying and conveying enzyme label plate are arranged in the detection cavity, the output end of first conveying belt is connected with the input end of second conveying belt, it further includes the feed hole and discharge hole being opened in enzyme label instrument body, the feed hole is located above first conveying belt, discharge hole is located in the side of second conveying belt output end, detection module is arranged above second conveying belt, first conveying belt is driven by first drive mechanism, second conveying belt is driven by second drive mechanism, the portable biological enzyme label instrument, it is realized to store the enzyme label plate of previous one at the same time to the enzyme label plate to be detected next time, taking and placing process are synchronized, improve work efficiency, it is favorable for the progress of large quantities of detection work.

1. The name of the design product: multifunctional enzyme label instrument. 2. The use of the design product: for nucleic acid, protein concentration, enzyme activity analysis and other conventional molecular detection; signal transduction research, cell signal event active oxygen, modification detection; cell viability, apoptosis, killing and other overall level analysis. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.

The application discloses a high-throughput screening method of phenylethanolamine-N-methyltransferase. Substrate norepinephrine and S-adenosyl methionine, PBS buffer, S-adenosyl-L-homocysteine hydrolase SAHH are added in a 96-well plate of a microplate reader, enzyme sample phenylethanolamine-N-methyltransferase is added, in a microplate shaking reactor, 20-50 DEG C, 500-1000 rpm, reaction for 1-3 h, 4 DEG C, 3700 rpm centrifugation, the supernatant is taken, compound NDCC is added again, reaction for 0-90 min, during the reaction, the microplate is taken out every 1-30 min, and the microplate reader is used to detect the absorbance value; according to the absorbance value change, the enzyme activity is defined as the change of OD405nm absorbance value per minute * 1000000 under specific conditions. The application has the characteristics of simple operation, low cost and accurate detection, and has far-reaching significance for the directed evolution of phenylethanolamine-N-methyltransferase.

This invention discloses a rapid detection method for protein in feed based on wet chemistry. The method includes the following steps: (1) mixing the feed sample with the sample extraction buffer, shaking to extract, centrifuging, and taking the supernatant; (2) adding the working solution of the protein standard and the supernatant to the diquinoline formic acid working solution, incubating, and measuring the absorbance value at a wavelength of 562 nm using an ELISA reader to obtain the absorbance values ​​of the protein standard and the supernatant; plotting a standard curve with the concentration of the protein standard as the abscissa and the absorbance value as the ordinate, and obtaining a linear regression equation; substituting the absorbance value of the supernatant into the linear regression equation to calculate the protein concentration in the supernatant, and then calculating the protein content in the feed sample based on the extraction buffer volume in step (1). This invention achieves a high degree of consistency between the detection results and the Kjeldahl nitrogen determination method, while significantly shortening the detection time of a single sample to less than 1 hour.

The present application relates to a kind of feed additive component detection device and detection method;The feed additive component detection device includes the following equipment optimized by parameter: muffle furnace, microwave digestion instrument, high-speed centrifuge, ultrasonic extraction instrument, electronic balance, with online enrichment liquid chromatograph, liquid chromatography-tandem mass spectrometer, atomic absorption spectrometer, near infrared spectrometer, laser particle size analyzer, inductively coupled plasma mass spectrometer, microbiological incubator, colony counter, portable near infrared spectrometer, portable liquid chromatograph, portable enzyme label instrument, sample pretreatment is carried out by above-mentioned equipment, multiple components are synchronously detected, detection precision control, trace component detection, new additive detection and on-site rapid detection, compared with prior art, the present application is accurate and efficient, can multiple components be synchronously detected, while detection precision is significantly improved, can be suitable for new additive high-precision detection.

This utility model discloses a sterilization system for internal disinfection of an ELISA reader, including an electric tray movably mounted on the ELISA reader. A detection tray is mounted on the upper limit of the electric tray. The ELISA reader has a mounting frame inside, which is located in the mounting cavity at the top of the electric tray. The bottom of the mounting frame has an identification mechanism and a disinfection mechanism. Both the identification mechanism and the disinfection mechanism are directly opposite the detection tray. The identification mechanism is linked with the disinfection mechanism and the electric tray through an external computer. When the identification mechanism detects leakage of liquid sample, the external computer activates the disinfection mechanism and locks the electric tray for closed disinfection, preventing secondary contamination and facilitating the cleaning of the electric tray.

The application provides a full-wavelength enzyme marker light path system based on a multi-spectrum LED light mixing module, relates to the technical field of biochemical detection equipment, and outputs wide-spectrum light through a multi-spectrum LED array, forms small-divergence-angle parallel light after beam shaping of a collimating lens array, and then forms uniform parallel wide-spectrum light beams after light condensation of a Fresnel lens, beam homogenization of an optical integrator and collimation of a collimating lens; after focusing and beam limiting of a beam shaping module, the light beams are incident to a light splitting module; the light beams are rotated and split through a concave diffraction grating to realize fiber output of 200-1000nm continuous monochromatic light; the monochromatic light output by the fiber is incident to a measurement module, is received by a detector after irradiating a sample, and finally the absorbance of the sample at a corresponding wavelength is calculated. The application replaces a traditional xenon lamp with multi-spectrum LED array light mixing, cooperates with optimized beam shaping, light splitting and measurement light paths, and fundamentally solves the problems of short service life, uneven energy, poor stability and large drift of the light source of the existing enzyme marker.

A ready-to-use assay kit for modular strip-well plates employs detachable strips whose wells contain co-located, mutually reactive reagents kept apart during storage by an in-well sequestration structure that encapsulates at least one reagent. Upon addition of an aqueous sample, buffer, or water, the structure releases (e.g., rupturable capsules / microcapsules or a phase-change encapsulant), enabling in-well mixing to form a reaction mixture that yields a detection signal. The kit is configured to operate without transfer of reagents between wells prior to reading. Optional features include in situ lyophilization with lyoprotectants, controlled residual moisture, and an inert-gas headspace. Calibrator and control strips may be provided. Detection can be by absorbance, fluorescence, or luminescence using a plate reader.

This invention discloses a method for screening drugs against Japanese encephalitis virus, which uses the main protease NS3 of Japanese encephalitis virus. pro The activity reporter plasmid and the main protease NS3 of Japanese encephalitis virus pro Expression plasmids were used to screen for drugs against Japanese encephalitis virus; NS3 pro Active reporter plasmids and NS3 pro The expression plasmid was co-transfected into HEK-293T cells. The fluorescence intensity of green fluorescent protein (EGFP) was detected using an inverted fluorescence microscope and a multi-functional microplate reader to predict NS3. pro The activity of NS3, the main protease of JEV, can be used to screen for its activity. pro The purpose of this invention is to screen anti-JEV compounds using active reporter plasmids. The inhibitory effect of the compounds can be observed more intuitively and easily by utilizing fluorescence intensity, providing a powerful tool for screening broad-spectrum anti-JEV compounds.

The present invention relates to a microplate reader comprising a receiving device for holding a microplate having a plurality of wells, and an optical detector for detecting optical radiation at each of the wells of a microplate held in the receiving device. The receiving device is movably arranged in at least one horizontal spatial direction via a positioning mechanism to position the received microplate relative to the optical detector for successive measurements at different wells. The element carrier according to the invention, comprising functional elements, is positioned directly above the microplate and enables radiation transport, processing, measurements, and the like by means of additional components.

1. Name of the product in this design: Microplate reader. 2. Purpose of this design: For clinical medical testing. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.