138 results about "Microplate Reader" patented technology

The invention discloses a fluorescent colorimetric sensor for detecting circulating tumor cells and a preparation method of the fluorescent colorimetric sensor. The preparation method comprises the following steps: S4, adding a prepared detection antibody modified with horse radish peroxidase and used for detecting the circulating tumor cells, and incubating; s5, adding a Tris buffer solution and a H2O2 solution, and incubating; s6, preparing an EuMOF nanoflower solution; and S7, adding the EuMOF nanoflower solution and 3, 3 ', 5, 5'-tetramethyl benzidine to react, detecting the fluorescence intensity in the presence of circulating tumor cells with different concentrations by using a luminoscope, detecting the ultraviolet absorbance value in the presence of circulating tumor cells with different concentrations by using a multifunctional microplate reader, and constructing a fluorescence intensity-concentration working curve and an ultraviolet absorbance value-concentration working curve. The method is used for qualitative and quantitative analysis and detection of the circulating tumor cells, realizes bimodal signal response to the circulating tumor cells, and has high sensitivity and simplicity and convenience in operation.

A novel microplate reader based on a grating module and an optical filter module comprises an electric microplate reader body, the grating module, the optical filter module, a motor reducing mechanism, a detection mechanism, a first detection control circuit and a second detection control circuit, the motor reducing mechanism is mounted at the rear side end in the detection bin, a supporting rod and a connecting rod are mounted at the front side end of a rotating shaft of the motor reducing mechanism, and the rear side ends of the grating module and the optical filter module are mounted together with the two ends of the front side of the connecting rod respectively; the detection mechanism comprises an adjustable resistor and a driven rubber wheel, the driven rubber wheel is installed on an adjusting handle of the adjustable resistor, and the lower portion of the adjustable resistor is installed on one side of a shell of the motor speed reducing mechanism. The first detection control circuit and the second detection control circuit are arranged in an element bin of the microplate reader body and are electrically connected. According to the utility model, convenience is brought to workers, the equipment fund investment of related departments is correspondingly saved, and a better detection effect can be realized on samples. Based on the above, the system has a good application prospect.

The invention relates to a method for detecting activity of gamma-polyglutamic acid hydrolase and application. The method comprises the following steps: (1) preparing an enzymolysis reaction solution and a blank reaction solution; (2) preparing an NAC / OPA mixed solution; (3) adding an NAC / OPA mixed solution into the enzymatic hydrolysis reaction solution to obtain a reaction solution to be detected; (4) drawing a standard curve by taking L-glutamic acid as a standard substance; and (5) detecting the fluorescence intensity of the blank reaction solution and the reaction solution to be detected by using a microplate reader, and then calculating the activity of the gamma-polyglutamic acid hydrolase according to the fluorescence intensity and the standard curve. The method for detecting the activity of the gamma-polyglutamic acid hydrolase is high in detection speed, detection of a single sample can be completed within 25 min, and nearly real-time process monitoring is achieved. And the method has the advantages of reliable detection result and high accuracy, can be used for monitoring the enzyme activity of the gamma-polyglutamic acid hydrolase in the gamma-polyglutamic acid preparation process in real time and guiding the optimization of the fermentation process, and has extremely high industrial application value.

The invention relates to the technical field of biological detection, in particular to a miniaturized portable fluorescence microplate reader for instant fluorescence detection, and aims to provide a fluorescence detection scheme which is portable, high in sensitivity and quick in response. The detector integrates an MCU microcontroller, a voltage conversion module, a light source driving module, a motor driving module, a photoelectric detection module, an AD sampling module, an automatic temperature control module based on a PID algorithm, a signal processing system and a data processing system. High-sensitivity and high-resolution detection of a fluorescent sample can be realized, the concentration of the sample can be detected in real time, and the change process of the concentration of the sample can be presented through a kinetic curve. Through the optimization of the structure, optics and electronic circuits, the instrument is more miniaturized and has portability. Due to the advantages of portability, high sensitivity, quick response and the like, the kit has wide application prospects in multiple fields of basic research of nucleic acid, gene expression, sequence analysis, transgenic detection and the like.

The invention relates to the technical field of microplate readers, and discloses a multifunctional microplate reader which comprises a full-wavelength microplate reader body, a detection cavity used for detecting a sample is formed in the full-wavelength microplate reader body, and a microplate used for placing the sample is arranged in the detection cavity. The full-wavelength microplate reader body is rotationally connected with a cover plate for sealing the detection cavity through a rotating shaft, the full-wavelength microplate reader body is further provided with a control panel for controlling the device to be opened and closed, a sample taking and placing assembly is arranged in the detection cavity, and a cleaning assembly is arranged on the sample taking and placing assembly. And a wiping assembly is arranged on the microporous plate. The device effectively avoids the problem that the accuracy of a sample detection result is affected due to the fact that an existing device is difficult to clean the surface of the microporous plate, and is high in practicability.

The invention discloses a monoluciferase-based ADCP biological activity detection method, which comprises the following steps: separating a mononuclear cell from a human peripheral blood mononuclear cell as an effector cell, phagocytizing a Raji cell transfected by Luciferase as a target cell by the mononuclear cell, acidifying and degrading the Raji cell in a phagocytic body, and degrading the Luciferase so as to attenuate a light-emitting signal, so that the ADCP biological activity is detected. The ADCP biological activity of the antibody therapeutic drug is accurately quantified by detecting the weakening of the light-emitting signal. According to the present invention, the method only needs to separate the mononuclear cells and perform Luciferase infection on the target cells, and only needs to add the luciferase substrate after the phagocytosis process, such that the luminescence value can be read by using the microplate reader, the operation process is simple, the steps are less, the time consumption is short, the high-throughput screening is achieved, and the new direction is provided for the ADCP detection.

The invention discloses a method for detecting multiple biomarkers based on a nano-enzyme chemiluminescence technology, and the method comprises the following steps: preparing iron oxide nano-particles as a nano-enzyme core, and coating the surfaces of the nano-particles with substrate molecules to form a nano-enzyme-substrate compound; mixing and incubating the nano enzyme-substrate compound and a sample to be detected, adding a hydrogen peroxide solution, transferring to a microplate, and collecting a chemiluminescence signal by using a microplate reader; extracting characteristic parameters including peak value luminous intensity, peak reaching time, half-peak width, rising rate, attenuation rate, integral intensity and / or wavelength ratio in the collected luminous curve, wherein the characteristic parameters comprise the peak value luminous intensity, the peak reaching time, the half-peak width, the rising rate, the attenuation rate and the integral intensity; and constructing and training a multi-layer neural network model so as to predict the concentration of the multiple biomarkers based on the characteristic parameters of the sample data. By means of the scheme, multiple biomarkers can be detected at the same time, and the detection accuracy is improved.

The invention discloses a CRISPR (clustered regularly interspaced short palindromic repeats)-based homogeneous chemiluminescence kit as well as a preparation method and a use method thereof, and belongs to the technical field of nucleic acid detection. The kit comprises a Cas enzyme, crRNA, a chemiluminescence reporter molecule, a free radical scavenger and hydrogen peroxide. The chemiluminescent reporter molecule comprises a reporter molecule modified with a chemical group for coupling, one end of the reporter molecule is connected with a peroxidase-like catalytic activity center for reacting with peroxide to generate active free radicals, and the other end of the reporter molecule is connected with a luminescent substrate for reacting with the active free radicals to generate luminescence. According to the kit, a multifunctional microplate reader can be used for collecting chemiluminescence signals for quantifying the concentration of target nucleic acid. The kit is simple in operation steps and short in detection time, does not need light source excitation, and avoids self-luminous interference of a biological sample matrix, so that the kit has more excellent sensitivity and detection dynamic range.

The utility model provides a dairy product detection workstation. The dairy product detection workstation comprises oscillation equipment, centrifugal equipment, reagent caching equipment, constant temperature equipment, a microplate reader and transfer equipment, the oscillation equipment is used for performing oscillation treatment on the container filled with the dairy products; the centrifugal equipment is used for performing centrifugal treatment on the centrifugal tube; the reagent caching equipment is used for storing a standard sample and a test reagent for testing, and the transfer equipment is used for mixing the test reagent with the standard sample and a dairy product sample respectively to obtain a first mixed solution and a second mixed solution; the constant-temperature equipment is used for heating and incubating the first mixed solution and the second mixed solution contained in the microwell plate; the microplate reader is used for detecting the incubated first mixed solution and second mixed solution to obtain the content of aflatoxin in the dairy product. And the transferring equipment can realize the transfer of the vessel and the solution among the equipment in the detection process. The dairy product detection workstation disclosed by the utility model can realize automation of aflatoxin detection, is high in detection efficiency and saves manpower.

The invention discloses an automatic polypeptide editing and synthesizing platform and an automatic synthesizing method. The automatic polypeptide editing and synthesizing platform comprises a working platform, and a synthesizing mechanism, a nitrogen blowing and liquid sucking mechanism, an automatic liquid injecting mechanism, a separating and detecting mechanism, a freezing treatment mechanism, a shaking table mixing mechanism, a centrifugal mechanism, a test tube rack mechanism and an enzyme labeling mechanism are arranged on the working platform; the mechanical arm mechanism is used for operating and synthesizing polypeptide; after the synthesis mechanism synthesizes a sample, the mechanical arm mechanism grabs a test tube from the test tube rack mechanism and receives the sample from the automatic liquid injection mechanism, and the sample is automatically operated and processed among the corresponding mechanisms; wherein the nitrogen blowing and liquid suction mechanism, the automatic liquid injection mechanism, the separation and detection mechanism and the enzyme labeling mechanism can be matched with the mechanical arm mechanism to automatically process samples. According to the structure, the whole process from polypeptide synthesis, purification, freeze-drying, dissolving agent filling, dialysis, centrifugal concentration to final elisa instrument characteristic testing can be completed on a unified platform.

The invention discloses a probe combination for detecting a mitochondrial respiratory chain super complex based on a proximity ligation assay and a hybridization chain reaction and application, and belongs to the field of probe detection. Two adjacent protein subunits capable of reflecting the structural integrity of the hypercomplex are determined as bases, the antigen-antibody combination principle is utilized to promote the antigen-antibody which recognizes and combines the bases to initiate proximity ligation reaction (PLA) due to nucleic acid labeling, cascade amplification of detection signals is realized according to the hybridization chain reaction (HCR) principle, and the detection sensitivity of the hypercomplex is improved. The activity of the oxidative phosphorylation system is judged through the structural integrity of the super-complex, the defects and limitation of a traditional detection method are overcome, and rapid, high-sensitivity and accurate detection on the structure of the super-complex is achieved. Meanwhile, the cells are fixed on a 96-hole fluorescent plate, and the structural integrity of the mitochondrial hypercomplex is detected by using a multifunctional microplate reader, so that parallel detection on 96 samples at the same time is finally realized, and digitization and high throughput of detection are realized.

This invention relates to the field of cell detection equipment technology, specifically to a detection device for bladder cancer metabolomics biomarkers. The device includes an ELISA reader, a moving stage, and well plates housed within it. It also includes a defoamer mounted on the outer wall of the ELISA reader. The defoamer comprises a fixing part, a pair of transmission parts, and a striking part and an air blowing part disposed within the fixing part. This bladder cancer metabolomics biomarker detection device, through a mechanical linkage design between the moving stage and the defoamer, uses a gear-driven toothed plate to move a dial-driven bending plate up and down, causing a striking rod to strike the well plate. Combined with synchronous airflow from the air blowing part, a protrusion pushes a piston plate within a second groove, directing airflow from the nozzle. This achieves automated removal of air bubbles from the wells before ELISA reader detection. The dual defoaming effect of striking vibration and airflow impact eliminates optical interference, ensuring the accuracy of the detection values ​​and simplifying the detection process.

The invention provides a method for detecting the dissolution rate of drug nanocrystals based on an aggregation-induced fluorescence probe, and belongs to the field of drug analysis. Comprising the steps of construction of an AIE probe-nanocrystal hybrid system, establishment of a fluorescence intensity-drug solid content standard curve, determination of the dissolution rate of the nanosuspension and the like. The amount of undissolved medicine is directly reflected through a solid-state fluorescence signal, and the adsorption error of a filter membrane is thoroughly eliminated; the microplate reader can online monitor the change of the fluorescence intensity of a plurality of samples along with time at one time; meanwhile, the detection method can be compatible with standard dissolution devices such as a paddle method; complex test conditions such as pH gradient dissolution are supported; compared with the prior art, the detection efficiency of the final implementation effect is improved by 5-30 times, the manual intervention of operation is reduced by 80%, the equipment and solvent cost is reduced by 80%, and the HPLC is only used for the establishment process of the fluorescence intensity-drug solid content standard curve; and the problem of too long time consumption of HPLC detection is substantially solved.

The utility model belongs to the technical field of microplate readers, and particularly relates to a microplate reader with a cleaning function, which comprises a microplate reader main body with a detection port on one side, a limiting plate is fixedly connected with the microplate reader main body on one side of the detection port, an elisa plate is slidably connected onto the limiting plate, a hidden groove is arranged on one side of the microplate reader main body, and the hidden groove is provided with a cleaning groove on the other side of the microplate reader main body. The hidden groove of the microplate reader main body is fixedly connected with a [-shaped track frame, the track frame is in sliding connection with a cleaning mechanism for cleaning an elisa plate, and the cleaning mechanism comprises an L-shaped plate which is in sliding connection with the track frame. The L-shaped plate, the vacuum pump, the pipeline, the valve, the hard pipe, the vacuum suction head and the like are arranged in the cleaning mechanism, and the rubber sheet is fixed on the upper surface of the sponge and can be adsorbed on the vacuum suction head, so that quick disassembly and assembly of the sponge are realized, the sponge is convenient to replace, and the problem that the sponge is inconvenient to replace in a device at the present stage is solved.

1. The name of the design product: microplate reader. 2. The use of the design product: microplate reader is used for sample measurement analysis, and is used for reading various information contained in the microplate. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view. 5. Other changes in the state of the case need to be explained: each reference figure shows the light-emitting state of the progress bar panel when working in the microplate reader.

The embodiment of the invention relates to a to-be-detected item typesetting method and device, electronic equipment and a storage medium, and the method comprises the steps: under the condition that a target set type is an enzyme immunoassay item set, according to preset enzyme immunoassay strategy information and item names corresponding to all to-be-detected items, setting the to-be-detected items according to the preset enzyme immunoassay strategy information; performing layout sorting on each experiment number corresponding to the plurality of items to be detected, and adding a fixed position before the first experiment number and / or after the last experiment number of each item to be detected, so as to generate a first layout file suitable for the microplate reader; under the condition that the target set type is a fixed electrophoresis type item set, according to preset electrophoresis typesetting information, item names corresponding to all the to-be-detected items and the number of experiment numbers contained in all the to-be-detected items, experiment numbers contained in all the to-be-detected items are distributed, and generating a second layout file suitable for the immunofixation electrophoresis apparatus. The processing efficiency of to-be-detected items can be improved, and the risk of manual operation errors is reduced.

The invention relates to a method for determining enzyme activity of ODC1 (ornithine decarboxylase 1), which comprises the following steps: digesting, centrifuging and resuspending cells, and taking a part of sample to detect protein concentration; the method comprises the following steps: cracking cells by using a specially-made enzyme activity cell lysis solution and a protein lysis solution, and ensuring full cracking through ultrasonication; carrying out quantitative analysis on the protein in the lysate by adopting a BCA kit; in the enzyme activity detection stage, a cell lysis solution and an enzyme activity detection working solution containing a specific fluorescent probe (CB6) are mixed and react at 37 DEG C for a certain time, and then the change of fluorescence intensity is determined by a multifunctional fluorescence microplate reader, so that the enzyme activity of the ODC1 is evaluated. According to the method, accurate determination of the ODC1 enzyme activity is realized by optimizing cell treatment, protein quantification and enzyme activity detection processes. The method has the advantages of simplicity and convenience in operation, high sensitivity, good specificity and the like, and is suitable for the fields of basic research, drug screening and the like related to the ODC1 enzyme activity.

The invention relates to an illumination system (10), in particular for a microplate (3) in a microplate reader (1), with a light source (12) for emitting optical radiation, which is configured within the illumination system (10) as a beam path (21) with an optical axis (22) and has a radiation cross-section that varies, in particular along the beam path (21), with a field aperture (14) which is arranged directly or indirectly adjacent to the light source (12) and has an aperture opening (14a) for the beam path (21), with an aperture diaphragm (16) which is arranged along the optical axis (22) at a distance (23) from the field aperture (14) and has a further aperture opening (16a) for the beam path (21), and with at least one lens which is arranged along the optical axis (22) in the beam path (21).The invention is characterized in that a central aperture (19) can be arranged in the beam path (21) within the distance (23) between the luminous field aperture (14) and the aperture aperture (16), wherein the central aperture (19) has an aperture area (20) with reduced transmission, in particular to the point of complete transmission suppression, in a region of the optical axis (22). The invention further relates to a microplate reader (1) and a method for generating an image of biological or chemical samples with a microplate reader (1).

The invention discloses a method for rapidly detecting the composite toxicity of micro-nano plastic and coexisting pollutants, which comprises the following steps of: preparing a working bacterial suspension, namely culturing bacteria and separating and resuspending thalli; poison exposure: sequentially adding the pollutant solution, the micro-nano plastic dispersion liquid and the working bacterium suspension into each hole of a light-proof 96-hole plate to form an exposure solution; detecting the luminous intensity: detecting the luminous intensity of each hole by using a microplate reader at high flux, and calculating the luminous inhibition ratio; and compound toxicity judgment: judging the action type and the action intensity of the compound toxicity by using an independent action model. By combining a Q67 toxicity test and a microwell plate high-throughput detection technology, the action type and the action strength of the composite toxicity are judged by utilizing an independent action model, and the toxicity effects of dozens of composite exposure groups can be synchronously evaluated in a single exposure experiment of 60 minutes, so that the composite pollution risk evaluation efficiency is remarkably improved, and the evaluation cost is reduced.

The present application relates to the technical field of enzyme label instrument, specifically, it is a kind of portable biological enzyme label instrument and its using method, including enzyme label instrument body and the detection cavity being arranged in the inside of enzyme label instrument body, first conveying belt and second conveying belt for carrying and conveying enzyme label plate are arranged in the detection cavity, the output end of first conveying belt is connected with the input end of second conveying belt, it further includes the feed hole and discharge hole being opened in enzyme label instrument body, the feed hole is located above first conveying belt, discharge hole is located in the side of second conveying belt output end, detection module is arranged above second conveying belt, first conveying belt is driven by first drive mechanism, second conveying belt is driven by second drive mechanism, the portable biological enzyme label instrument, it is realized to store the enzyme label plate of previous one at the same time to the enzyme label plate to be detected next time, taking and placing process are synchronized, improve work efficiency, it is favorable for the progress of large quantities of detection work.

1. The name of the design product: enzyme label instrument (laboratory). 2. The use of the design product: for laboratory testing instruments. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.

The utility model discloses an elisa plate reader convenient to clean, which comprises an elisa plate reader main body, the elisa plate reader main body is provided with a control panel, a sample loading plate is mounted at a sample inlet of the elisa plate reader main body, a limiting plate is hinged to the sample loading plate, a limiting square groove is formed in the limiting plate, an elisa plate is placed in the limiting square groove, and the elisa plate is arranged in the limiting square groove. A cleaning assembly is arranged above the elisa plate and comprises a first module, a second module and a cleaning brush, the cleaning brush is connected to the second module in an up-down sliding mode, the second module is installed at the output end of the first module, the first module is installed on the microplate reader body, and the second module is installed at the output end of the second module. The sample loading plate and the limiting plate are automatically cleaned through the cleaning assembly, the cleaning efficiency is improved, the limiting plate is hinged to the sample loading plate, separate cleaning is facilitated, and cleaning is cleaner.

The invention discloses a thioredoxin reductase detection method, which comprises the following steps: preparing a hairpin DNA probe HP solution, an H1 solution and an H2 solution, and carrying out annealing treatment; a pretreatment hairpin type DNA probe HP solution, a pretreatment hairpin type DNA probe H1 solution and a pretreatment hairpin type DNA probe H2 solution are obtained; mixing the three pre-treated hairpin DNA probe solutions, adding an NADPH solution and a sample to be detected, and incubating at 37 DEG C to obtain a solution to be detected; and collecting a fluorescence signal of the to-be-detected solution through a multifunctional microplate reader, and calculating the concentration of thioredoxin reductase through a linear equation: Y = 730.7 X + 2000.2, Y representing the fluorescence signal and X representing the logarithm LogCTrxR of TrxR concentration. According to the method disclosed by the invention, the detection sensitivity of the TrxR is remarkably improved, and the method is applied to imaging of the TrxR; the method has the advantages of good selectivity, high sensitivity, real-time monitoring and the like.

The application discloses a kind of transfer factor detection equipment and method, it is related to biological detection technical field, including microplate reader, detection chamber, microporous plate, detection component, the inner wall of the detection chamber is symmetrically provided with chute, and the inner wall of chute is connected with tray with sliding, the bottom of the tray is provided with push positioning mechanism, to in push detection, the detection position of the microporous plate is fixed with adhesion.This kind of transfer factor detection equipment, in push detection process, can be rotated by driving tray to drive gear, using the cooperation of guide groove and shaft, four positioning blocks are synchronously moved inward, so that four positioning blocks can be used to adhere positioning four sidewalls of microporous plate when being pushed to detection position, guarantee the accuracy of microporous plate position in detection process, in turn guarantee the accuracy of subsequent detection result of the equipment.

The present invention provides a multi-wavelength-based HBsAg detection method, including: detecting the light absorption value of a sample to be tested for HBsAg by a sandwich ELISA method using a multi-wavelength microplate reader to obtain the light absorption data of the sample to be tested for HBsAg at multiple wavelengths; determining the OD value of the sample, the OD value of the negative control, and the OD value of the positive control at dual wavelengths based on the light absorption data at wavelengths of 450 nm and 630 nm; determining whether the first detection result is valid within the preset concentration range of HBsAg based on the OD value of the negative control and the OD value of the positive control; if the first detection result is invalid within the preset concentration range of HBsAg, determining the second detection result of the sample to be tested for HBsAg based on the mapping relationship between the light absorption data at multiple wavelengths and the detection result of chemiluminescence method. The present invention improves the sensitivity, accuracy, and reliability of hepatitis B surface antigen detection at low concentrations.

1. The name of the design product: multifunctional enzyme label instrument. 2. The use of the design product: for nucleic acid, protein concentration, enzyme activity analysis and other conventional molecular detection; signal transduction research, cell signal event active oxygen, modification detection; cell viability, apoptosis, killing and other overall level analysis. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.

The utility model belongs to the technical field of optical fiber assemblies, and particularly relates to an optical fiber assembly suitable for a microplate reader, which comprises a first optical fiber group and a second optical fiber group which need to be butted, and the first optical fiber group is sequentially provided with a first fiber core, a first sheath and a first joint from inside to outside; the second optical fiber group is sequentially provided with a second fiber core, a second sheath and a second connector from inside to outside, the first fiber core protrudes out of the front end of the first connector, and the exterior of the first connector is elastically connected with a floating sleeve matched with the protruding part of the first fiber core; it can be understood that the second fiber core is designed to be of an inward sinking structure, the first fiber core is designed to be of a protruding structure, the connecting face of the second fiber core and the first fiber core is arranged in the second connector to reduce the risk of light leakage, and the floating sleeve is additionally arranged outside the first fiber core to improve the protection performance; and meanwhile, the first magnetic blocks are inserted into the positioning grooves to prevent rotation and radial deviation, and the arc-shaped surface adsorption design of the first and second magnetic blocks is matched to increase the contact area, so that the suction force and the connection reliability are enhanced.

An adjustment device (100) for a microplate reader (1000), includes: an actuation mechanism (10) connected to a housing (1000A) of the microplate reader (1000); an adjustable aperture (20) including an aperture opening (21), wherein the adjustable aperture (20) is driven to move in an axial direction by an actuation mechanism (10); and a diaphragm opening adjusting member (30) configured to be movable relative to the center of the aperture opening (21) so as to define an adjustable diaphragm opening (22) in the adjustable aperture (20), where the diaphragm opening adjusting member (30) is movable between a first position and a second position as the adjustable aperture (20) moves towards or away from a microplate (201) in the axial direction, and the size of the adjustable diaphragm opening (22) in the first position is greater than the size of the adjustable diaphragm opening (22) in the second position. The adjustment device (100) can change the size of the adjustable diaphragm opening (22) while the axial position of the adjustable aperture (20) changes. In addition, a microplate reader (1000) includes an adjustment device (100).

The invention develops a Brucella colorimetric detection method based on DNAWalker driving double signal amplification. The detection principle is as follows: target DNA triggers AP hybridization to form Y-type connection with CP, an Nt.BbvCI restriction enzyme cutting site is exposed, DNAWalker is released through restriction enzyme cutting circulation, and target circulation amplification is realized. Meanwhile, PDANS (palladium-iron-nano particles) is synthesized through Fe < 2 + > / H2O2 Fenton reaction, a pH indicator is loaded through silver nano particle modification (PDANS (at) Ag), and a signal is enhanced. An amplified product is combined with PDANS (at) Ag through a magnetic bead sandwich structure, remarkable color change is caused, and quantitative detection can be performed through a smart phone App (such as' Color Grab ') or a microwell plate reader. The method is easy and convenient to operate, high in sensitivity and suitable for on-site rapid identification of Brucella.

The invention relates to the technical field of microbial screening, in particular to a method for high-throughput screening of flavone-transformed bacteria and a screening system thereof. The method comprises the following steps: firstly, inoculating a to-be-detected strain into a basal culture medium containing a flavone substrate for fermentation, then scanning fermentation broth at two different time points by using a full-wavelength microplate reader through a quartz microplate, calculating an absorbance difference value at at least one preset wavelength, and finally, calculating the absorbance difference value at least one preset wavelength. The strains with the absorbance difference larger than a preset threshold value are screened as primary screening positive strains. Compared with the traditional technology, the method has the advantages that the screening efficiency is improved by hundreds of times, the sample consumption is reduced by 75%, the industrial bottleneck that multi-structure flavonoids need to be repeatedly developed is broken through, and an innovative tool is provided for microbial resource development.