513 results about "Microplate Reader" patented technology

A reflective light imaging system for use in high-throughput screening of samples disposed in multiple-well plates. The system can include a set of mirrors and lenses. The first mirror has a central aperture through which light from the object passes. The first mirror has a concave reflective surface that faces the image plane. The next element is a second mirror with a convex reflective surface. The system can include an aberration corrective system positioned between the second mirror and the image plane, and an optical sensor near the image plane. Light from an object passes through the central aperture of the first mirror and is reflected off the convex surface of the second mirror. The light then strikes the reflective surface of the first mirror. The light from the first mirror is then collected by the aberration correction system and transmitted toward the image plane.

The invention discloses a Cpf1 reagent kit for quickly detecting nucleic acid of an African swine fever virus. The Cpf1 reagent kit comprises a Cpf1 detection system suitable for quickly detecting theAfrican swine fever virus, and an immune colloidal gold test strip, wherein the Cpf1 detection system comprises specific crRNA protein, specific Cpf1 protein and a single-chain DNA(ssDNA) reporting system in accordance with a p72 gene of the African swine fever virus, the specific crRNA is one or more of crRNAs from ASFV P72 crRNA1 to ASFV P72 crRNA10, and the sequence of the specific crRNA is SEQ NO.4 to SEQ NO.13; and the single-chain DNA(ssDNA) reporting system comprises ssDNA FQreporter for fluorescence detection of a microplate reader and/or ssDNA DB reporter for detecting the immune colloidal gold test strip. According to the Cpf1 reagent kit disclosed by the invention, for the first time, the Cpf1 is used for detecting the African swine fever virus, and has the advantages of beinghigh in sensitivity, high in specificity, short in time consumption, high in flux, independent of large-scale experiment equipment and the like. The advantages enable a detection method based on the immune colloidal gold test strip developed by the invention to be conveniently used in basic laboratories and breeding enterprises to be used for performing detection, identification and diagnosis on basic quick detection of the African swine fever.

The invention discloses a method for detecting tetracycline residues in milk and drinking water and belongs to the technical field of food safety. The method comprises the following steps of: constructing a detection system with known tetracycline concentration to obtain a light absorption difference standard curve, fitting the curve to obtain a regression equation, mixing milk or drinking water to be detected with an acetic acid solution to obtain a mixed solution, centrifuging and layering the mixed solution to obtain a supernatant liquid, adding a tetracycline aptamer solution, adding a nanogold solution after incubation, finally adding positive ion electrolyte, determining the difference of the light adsorption rates at the 650nm part and the 520nm part of an electrolyte standard solution and a blank control solution by a microplate reader, and calculating the content of the tetracycline in milk and drinking water to be detected under the control of the equation obtained by fitting the standard curve. The method is high in speed, low in cost, high in sensitivity, good in selectivity, and convenient to operate without depending on large-sized instruments or equipment, the lowest detection limit is 45.8nM, the defects that the existing aptamer is time-wasting due to marking and high in cost are overcome and the method has the advantages of high speed, sensitivity and low cost.

The invention relates to a high-throughput screening method for a high-yield monascus pigment strain. The method comprises the following steps of all mutated monascus suspensions to a deep-well plate for mixed culturing, performing high-throughput detection to obtain a mixture with the highest yield, performing plate separation and purification, and performing shake flask fermentation and detection to obtain a pure high-yield strain. The complete high-throughput screening method integrating a deep-well plate culturing technology and microplate reader detection is convenient, rapid, accurate and efficient.

The invention discloses novel coronavirus rapid detection test paper and a detection method. The novel coronavirus rapid detection test paper is composed of a sample pad, a colloidal gold pad, a reaction color development area, a water absorption area and a supporting plate; the sample pad and the water absorption pad are arranged on the supporting plate and located on the two opposite sides of the supporting plate respectively, and the sample pad is suction filter paper or a glass fiber membrane and used for bearing an object to be detected; a nitrocellulose membrane is fixed on the supporting plate, and the absorbent pad is absorbent paper; through double binding of a novel coronavirus antibody and a receptor ACE2 protein of a virus, a coronavirus protein antigen is specifically capturedand bound by adopting double enzyme-linked immunosorbent assay; the ACE2 antibody in the test paper reaction color development area is combined with biotin, avidin is connected with horse radish peroxidase, and through a biotin-avidin system, rapid judgment can be achieved through chemical color development, and the virus content can also be accurately detected through a microplate reader.

The invention provides a human respiratory syncytial virus antigen detection method based on magnetic separating and quantum dot labeling. The method comprises the steps that 1, immune nano magnetic beads resisting a human respiratory syncytial virus are prepared; 2, quantum-dot-labeled nano-probes resisting the human respiratory syncytial virus are prepared; 3, after a sample to be detected is dissolved with a sample processing solution, the immune nano magnetic beads resisting the human respiratory syncytial virus are added in a dissolved solution, magnetic separating is performed after full mixing and reacting are performed, washing is performed with a PBST buffer solution, the quantum-dot-labeled nano-probes resisting the human respiratory syncytial virus are added in obtained precipitates, magnetic separating is performed after reacting is performed, and after washing is performed with the PBST buffer solution, a fluorescence value is detected with a fluorescence microplate reader. Accordingly, the accurate, rapid and high-sensitivity method for detecting the human respiratory syncytial virus is established, and the very high practical value in the aspects such as clinical diagnosis, etiology identification and epidemiological investigation of the human respiratory syncytial virus is achieved.

The invention discloses a high throughput screening method of aminopeptidase and a high-yield strain thereof, relates to a high throughput screening method of aminopeptidase and a high-yield microorganism bacterial strain thereof. The method comprises the following steps of: vaccinating a single colony in the hole of a deep-hole plate I on a medium containing a seed liquid, performing shaking cultivation, vaccinating the seed liquid in the hole of the deep-hole plate I correspondingly into the hole of a deep-hole plate II equipped with a fermentation medium, performing shaking cultivation, and centrifuging the deep-hole plate II to obtain the supernatant crude enzyme; adding a buffer solution and a chromogenic substrate solution or fluorogenic substrate solution in the micropore of an elisa plate, adding the dilute crude enzyme to be tested, carrying out water-bath reaction for 10-30 minutes at 30-70 DEG C, adding a 30-50% acetic acid solution immediately after the water-bath reaction and terminating the reaction; and measuring the absorbance or fluorescence intensity of the reaction liquid by a microplate reader, and calculating the enzyme activity according the standard curve and the dilution ratio of the crude enzyme. According to the invention, the goals of high throughput cultivation, high throughput preparation of crude enzyme and the high throughput test of the enzyme activity are achieved.

The invention discloses a quick counting method for microcystis aeruginosa. The quick counting method comprises the following steps of: measuring the absorbancy of microcystis aeruginosa with different concentrations at wavelength of 680nm through a microplate reader, accurately counting frustules through a microscope at the same time, creating a good linear relation between the absorbancy and cell density, and quickly counting the microcystis aeruginosa in a sample to be measured through an obtained correction curve or regression equation. The simple, safe, effective and quick quantifying method for microcystis aeruginosa provided by the invention overcomes the defects of lack of algae monitoring and counting measures and low monitoring and counting efficiency in China.

The invention provides an Aspergillus galactomannan (GM) antigen immunoassay kit, comprising a solid phase carrier coated with GM antigen, an anti-GM antigen polyclonal antibody and a GM antigen standard product, the kit first GM antigen is coated on a solid phase carrier. During detection, the sample to be tested or GM antigen standard competes with the coated antigen for limited antibody binding sites, and then a color reaction occurs between the enzyme and the substrate, and the color depth of the measurement result It is negatively correlated with the concentration of the antigen to be tested. A standard curve can be drawn according to the GM antigen standard, and the concentration of the antigen to be tested can be calculated according to the standard curve equation. The kit has good sensitivity and specificity, the result coincides with the reference reagent in a high rate, and can provide more accurate and reliable test results. Its operation is simple and easy, and the detection is fast and sensitive. Inexpensive, the detection kit provides an effective tool for the quantitative detection of GM antigen.

The invention relates to a urease michaelis constant determination method, namely a urease-phenol red method, belonging to the technical field of biochemistry and application enzymology. The determination method provided by the invention is characterized in that a series of urea solutions with different concentrations act as a substrate, and phenol red acts as an indicating agent, urea is decomposed to generate ammonia under the action of urease michaelis, and pH value is increased; the color of a system becomes dark under the action of the phenol red indicating agent, the absorbancy is increased, and is determined by a spectrophotometer or a microplate reader. The enzymatic reaction velocity is in direct proportion to the increase of the absorbancy value, so that the reciprocal 1/delta A of the absorbancy increase value can replace 1/V in a linewaver-Burk equation 1/V=Km/(Vmax[S])+1/Vmax, and the reciprocal 1/[S] of the concentration of the substrate urea is plotted; the intercept -1/Km of a straight line on the X axis can be obtained via the linewaver-Burk equation, and the Km value is resolved.

A polarization validation microplate for validating fluorescence polarization readers includes a microplate housing and one or more sealed fluorescence polarization samples in the housing positioned for reading by a fluorescence polarization reader.

The invention relates to an apparatus and method for optically analysing samples contained in sample sites of a sample holder by means of fluorescence. The apparatus comprises a first light source comprising a plurality of individual light sources having narrow wavelength bands, means for further limiting wavelength bands of the light emitted by the individual light sources, means for guiding the reduced-wavelength light to the sample sites of the sample holder, and a detector for detecting light from the sample sites. According to the invention said means for further reducing the wavelength bands emitted by the individual light sources comprise a wavelength-tunable single monochromator. The invention allows manufacturing of a microplate reader having the capability for fluorescence measurements at a continuous wavelength range, while maintaining the cost of the device at a reasonable level.

The invention discloses Halomonas sp. capable of degrading phenols and a high-throughput screening method and application thereof. The Halomonas sp. PH4-5 is preserved under CGMCC 10666. A bacterial liquid is cultured with a 48-pore deep-pore board, phenol concentration is measured by 96-pore microplate reader plate spectrophotometry based on 4-amino antipyrine colorimetry, moderately halophilic bacteria efficient in degrading phenols are screened from 43 environmental samples contaminated by high salinity and organics, the Halomonas sp. is separated and verified from an optimal flora E3, and a salt tolerant degrading mechanism of the Halomonas sp. is studied. Studying results show that quick screening of environmental degrading bacteria provides methodological basis and also provides technical support for the treatment of high-salinity phenolic wastewater.

The invention relates to an analyzing method for detecting activity of soil xylanase, which comprises the following steps: firstly, weighting n sieved air dry soil samples into n thick test tubes, adding acetic acid buffer solution into each test tube, oscillating by a vortex oscillator, getting soil suspension into 96 micropore plates under the oscillation condition, adding 4-MUB-7-Beta-D-xyloside substrate solution into n-1 holes, and adding water with equal quantity into the other one hole so as to be used as non-substrate contrast, adding substrate solution with equal quantity and water with equal quantity into the (n+1)th hole so as to be used as soli-free contrast, oscillating and culturing under constant temperature; secondly, adding NaOH into the micropore plates to terminate the reaction after the culture is finished; thirdly, performing the fluorimetric determination to resultant of reaction by a multifunctional microplate reader; and fourthly, calculating the activity of the xylanase. Compared with the traditional method, the invention shortens the culture time, omits the operation procedures of filtration, and the like, and simplifies the operation steps; meanwhile, the determination data of fluorescent materials in the micropore plate can be obtained within 15s through the multifunctional microplate reader, huge samples are allowed to be simultaneously determined; and moreover, the invention has high accuracy and easy operation, stable and reliable result and good reproduction quality.

The invention discloses a method capable of rapidly, simply, conveniently and quantitatively detecting minimal inhibitory concentration, and the method comprises the following steps of: in accordance with the standard of CLSI (Clinical and Laboratory Standards Institute) (Version 2010), adding a fresh enterococcus suspension of a certain concentration into a sterile 96-well plate containing concentration gradient antibacterials for co-incubation; upon the ending of the 4-hour incubation, adding a fixed amount of fluorescent dyes SYTOX Green and DAPI (4,6-diamino-2-phenyl indole) to all the wells, protecting the wells from light for 15 minutes at room temperature, reading the fluorescent intensity of the two dyes in the wells by use of a fluorescent microplate reader respectively; after relevant background fluorescence is deducted, drawing a corresponding curve between bacterial fluorescence intensity ratio (Pdead/livel) and concentration of drug (CDrug), and determining the minimal concentration of drug corresponding to the moment the Pdead/livel is no longer fluctuated as the MIC (Minimal Inhibitory Concentration) of the antibiotic to the bacterium. The detection method provided by the invention has the characteristics of being simple, convenient, rapid and objective and being capable of performing mathematic statistics and analysis directly on detected data, becomes a new detection method for determining the minimal inhibitory concentration of antibacterials, and has a wide application prospect.

The invention discloses a method for measuring the antioxidant activity of lactic acid bacteria based on a cellular level and belongs to the technical field of microorganisms. According to the method, a full-wave fluorescent scanning type microplate reader with a 96-hole ferment plate based on the cellular level is utilized to replace a conventional spectrophotometer so as to measure the antioxidant capability of a treated sample; a fluorescent probe and a free-radical initiator are utilized to perform batched rapid and accurate measurement on lactic acid bacteria within 1 hour, so that a rapid detection method is provided for researching the antioxidant activity of the lactic acid bacteria at the cellular level; by utilizing the method, the antioxidant activity of the different strains of lactic acid bacteria can be measured within ultrashort time, and the antioxidant level of the lactic acid bacteria can be rapidly and accurately reflected.

The invention relates to a developing-process fungus 1,3-beta-D-glucan detection kit for human body fluid. The developing-process fungus 1,3-beta-D-glucan detection kit comprises a reaction main agent, a main agent compound solution, a sample treatment solution, heat-source-free water, a standard product and a quality control product, wherein the reaction main agent takes horseshoe crab blood cells as a main raw material and contains G factors, coagulase, coagulase zymogen and a polypeptide developing substrate; the polypeptide developing substrate is synthesized tripeptide or tetrapeptide with a Gly-Arg tail end connected with a PNA; the polypeptide developing substrate is subjected to enzyme digestion by adopting the coagulase; after the free paranitroaniline (PNA) is generated, a microplate reader is used for directly detecting so that a detection route is shortened and the cost is reduced; the microplate reader is used for carrying out a velocity-method enzyme kinetics detection method so that the sensitivity is relatively high when being compared with a nephelometry detection method; and the reaction main agent is not easily interfered by protein in a body fluid sample and medicines to generate non-specific turbidity, so that the probability of a false positive detection result is reduced and the detection accuracy is relatively high.

The invention discloses a method for quickly and specifically detecting the enzymatic activity of a phospholipid-depending factor X activator. A chromogenic substrate assay is adopted, a microplate reader is used, a linear reaction curve is obtained in a certain reaction time and a certain-activity standard substance range, a linear relation between a light absorption value of a product and the concentration of the phospholipid-depending factor X activator is established, and the corresponding enzymatic activity of a sample is calculated. In addition, the recovery rate and accuracy of the standard curve are carefully researched. The method is high in accuracy, simple in steps and convenient to impellent in a laboratory, and is suitable for quality control of X activator raw materials and hemocoagulase preparations.

The invention discloses a respiratory syncytial virus nucleic acid rapid detection kit based on CRISPR/Cas12a and a detection method. The CRISPR/Cas12a kit includes a CRISPR/Cas12a detection system; the CRISPR/Cas12a detection system includes a specific crRNA, CRISPR/Cas12a protein and a single-stranded DNA reporter system for respiratory syncytial virus; the specific crRNA is any one or more kinds designed and synthesized based on respiratory syncytial virus nucleic acid; and the single-stranded DNA reporter system includes a ssDNA FQ reporter for fluorescence detection of a microplate readerand/or a ssDNA DB reporter for immune colloidal gold test stripe detection. The CRISPR/Cas12a is used for detecting a nucleic acid sequence of respiratory syncytial virus for the first time, and thedetection kit has the advantages of high sensitivity, high specificity, short time-consuming, and no dependence on large-scale experimental equipment.