70 results about "Microplate Reader" patented technology

The invention relates to a method for detecting activity of gamma-polyglutamic acid hydrolase and application. The method comprises the following steps: (1) preparing an enzymolysis reaction solution and a blank reaction solution; (2) preparing an NAC / OPA mixed solution; (3) adding an NAC / OPA mixed solution into the enzymatic hydrolysis reaction solution to obtain a reaction solution to be detected; (4) drawing a standard curve by taking L-glutamic acid as a standard substance; and (5) detecting the fluorescence intensity of the blank reaction solution and the reaction solution to be detected by using a microplate reader, and then calculating the activity of the gamma-polyglutamic acid hydrolase according to the fluorescence intensity and the standard curve. The method for detecting the activity of the gamma-polyglutamic acid hydrolase is high in detection speed, detection of a single sample can be completed within 25 min, and nearly real-time process monitoring is achieved. And the method has the advantages of reliable detection result and high accuracy, can be used for monitoring the enzyme activity of the gamma-polyglutamic acid hydrolase in the gamma-polyglutamic acid preparation process in real time and guiding the optimization of the fermentation process, and has extremely high industrial application value.

The invention relates to the technical field of microplate readers, and discloses a multifunctional microplate reader which comprises a full-wavelength microplate reader body, a detection cavity used for detecting a sample is formed in the full-wavelength microplate reader body, and a microplate used for placing the sample is arranged in the detection cavity. The full-wavelength microplate reader body is rotationally connected with a cover plate for sealing the detection cavity through a rotating shaft, the full-wavelength microplate reader body is further provided with a control panel for controlling the device to be opened and closed, a sample taking and placing assembly is arranged in the detection cavity, and a cleaning assembly is arranged on the sample taking and placing assembly. And a wiping assembly is arranged on the microporous plate. The device effectively avoids the problem that the accuracy of a sample detection result is affected due to the fact that an existing device is difficult to clean the surface of the microporous plate, and is high in practicability.

This invention relates to the field of cell detection equipment technology, specifically to a detection device for bladder cancer metabolomics biomarkers. The device includes an ELISA reader, a moving stage, and well plates housed within it. It also includes a defoamer mounted on the outer wall of the ELISA reader. The defoamer comprises a fixing part, a pair of transmission parts, and a striking part and an air blowing part disposed within the fixing part. This bladder cancer metabolomics biomarker detection device, through a mechanical linkage design between the moving stage and the defoamer, uses a gear-driven toothed plate to move a dial-driven bending plate up and down, causing a striking rod to strike the well plate. Combined with synchronous airflow from the air blowing part, a protrusion pushes a piston plate within a second groove, directing airflow from the nozzle. This achieves automated removal of air bubbles from the wells before ELISA reader detection. The dual defoaming effect of striking vibration and airflow impact eliminates optical interference, ensuring the accuracy of the detection values ​​and simplifying the detection process.

The utility model belongs to the technical field of microplate readers, and particularly relates to a microplate reader with a cleaning function, which comprises a microplate reader main body with a detection port on one side, a limiting plate is fixedly connected with the microplate reader main body on one side of the detection port, an elisa plate is slidably connected onto the limiting plate, a hidden groove is arranged on one side of the microplate reader main body, and the hidden groove is provided with a cleaning groove on the other side of the microplate reader main body. The hidden groove of the microplate reader main body is fixedly connected with a [-shaped track frame, the track frame is in sliding connection with a cleaning mechanism for cleaning an elisa plate, and the cleaning mechanism comprises an L-shaped plate which is in sliding connection with the track frame. The L-shaped plate, the vacuum pump, the pipeline, the valve, the hard pipe, the vacuum suction head and the like are arranged in the cleaning mechanism, and the rubber sheet is fixed on the upper surface of the sponge and can be adsorbed on the vacuum suction head, so that quick disassembly and assembly of the sponge are realized, the sponge is convenient to replace, and the problem that the sponge is inconvenient to replace in a device at the present stage is solved.

1. The name of the design product: microplate reader. 2. The use of the design product: microplate reader is used for sample measurement analysis, and is used for reading various information contained in the microplate. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view. 5. Other changes in the state of the case need to be explained: each reference figure shows the light-emitting state of the progress bar panel when working in the microplate reader.

The embodiment of the invention relates to a to-be-detected item typesetting method and device, electronic equipment and a storage medium, and the method comprises the steps: under the condition that a target set type is an enzyme immunoassay item set, according to preset enzyme immunoassay strategy information and item names corresponding to all to-be-detected items, setting the to-be-detected items according to the preset enzyme immunoassay strategy information; performing layout sorting on each experiment number corresponding to the plurality of items to be detected, and adding a fixed position before the first experiment number and / or after the last experiment number of each item to be detected, so as to generate a first layout file suitable for the microplate reader; under the condition that the target set type is a fixed electrophoresis type item set, according to preset electrophoresis typesetting information, item names corresponding to all the to-be-detected items and the number of experiment numbers contained in all the to-be-detected items, experiment numbers contained in all the to-be-detected items are distributed, and generating a second layout file suitable for the immunofixation electrophoresis apparatus. The processing efficiency of to-be-detected items can be improved, and the risk of manual operation errors is reduced.

The invention relates to a method for determining enzyme activity of ODC1 (ornithine decarboxylase 1), which comprises the following steps: digesting, centrifuging and resuspending cells, and taking a part of sample to detect protein concentration; the method comprises the following steps: cracking cells by using a specially-made enzyme activity cell lysis solution and a protein lysis solution, and ensuring full cracking through ultrasonication; carrying out quantitative analysis on the protein in the lysate by adopting a BCA kit; in the enzyme activity detection stage, a cell lysis solution and an enzyme activity detection working solution containing a specific fluorescent probe (CB6) are mixed and react at 37 DEG C for a certain time, and then the change of fluorescence intensity is determined by a multifunctional fluorescence microplate reader, so that the enzyme activity of the ODC1 is evaluated. According to the method, accurate determination of the ODC1 enzyme activity is realized by optimizing cell treatment, protein quantification and enzyme activity detection processes. The method has the advantages of simplicity and convenience in operation, high sensitivity, good specificity and the like, and is suitable for the fields of basic research, drug screening and the like related to the ODC1 enzyme activity.

The invention relates to an illumination system (10), in particular for a microplate (3) in a microplate reader (1), with a light source (12) for emitting optical radiation, which is configured within the illumination system (10) as a beam path (21) with an optical axis (22) and has a radiation cross-section that varies, in particular along the beam path (21), with a field aperture (14) which is arranged directly or indirectly adjacent to the light source (12) and has an aperture opening (14a) for the beam path (21), with an aperture diaphragm (16) which is arranged along the optical axis (22) at a distance (23) from the field aperture (14) and has a further aperture opening (16a) for the beam path (21), and with at least one lens which is arranged along the optical axis (22) in the beam path (21).The invention is characterized in that a central aperture (19) can be arranged in the beam path (21) within the distance (23) between the luminous field aperture (14) and the aperture aperture (16), wherein the central aperture (19) has an aperture area (20) with reduced transmission, in particular to the point of complete transmission suppression, in a region of the optical axis (22). The invention further relates to a microplate reader (1) and a method for generating an image of biological or chemical samples with a microplate reader (1).

The invention discloses a method for rapidly detecting the composite toxicity of micro-nano plastic and coexisting pollutants, which comprises the following steps of: preparing a working bacterial suspension, namely culturing bacteria and separating and resuspending thalli; poison exposure: sequentially adding the pollutant solution, the micro-nano plastic dispersion liquid and the working bacterium suspension into each hole of a light-proof 96-hole plate to form an exposure solution; detecting the luminous intensity: detecting the luminous intensity of each hole by using a microplate reader at high flux, and calculating the luminous inhibition ratio; and compound toxicity judgment: judging the action type and the action intensity of the compound toxicity by using an independent action model. By combining a Q67 toxicity test and a microwell plate high-throughput detection technology, the action type and the action strength of the composite toxicity are judged by utilizing an independent action model, and the toxicity effects of dozens of composite exposure groups can be synchronously evaluated in a single exposure experiment of 60 minutes, so that the composite pollution risk evaluation efficiency is remarkably improved, and the evaluation cost is reduced.

The present application relates to the technical field of enzyme label instrument, specifically, it is a kind of portable biological enzyme label instrument and its using method, including enzyme label instrument body and the detection cavity being arranged in the inside of enzyme label instrument body, first conveying belt and second conveying belt for carrying and conveying enzyme label plate are arranged in the detection cavity, the output end of first conveying belt is connected with the input end of second conveying belt, it further includes the feed hole and discharge hole being opened in enzyme label instrument body, the feed hole is located above first conveying belt, discharge hole is located in the side of second conveying belt output end, detection module is arranged above second conveying belt, first conveying belt is driven by first drive mechanism, second conveying belt is driven by second drive mechanism, the portable biological enzyme label instrument, it is realized to store the enzyme label plate of previous one at the same time to the enzyme label plate to be detected next time, taking and placing process are synchronized, improve work efficiency, it is favorable for the progress of large quantities of detection work.

1. The name of the design product: enzyme label instrument (laboratory). 2. The use of the design product: for laboratory testing instruments. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.

The utility model discloses an elisa plate reader convenient to clean, which comprises an elisa plate reader main body, the elisa plate reader main body is provided with a control panel, a sample loading plate is mounted at a sample inlet of the elisa plate reader main body, a limiting plate is hinged to the sample loading plate, a limiting square groove is formed in the limiting plate, an elisa plate is placed in the limiting square groove, and the elisa plate is arranged in the limiting square groove. A cleaning assembly is arranged above the elisa plate and comprises a first module, a second module and a cleaning brush, the cleaning brush is connected to the second module in an up-down sliding mode, the second module is installed at the output end of the first module, the first module is installed on the microplate reader body, and the second module is installed at the output end of the second module. The sample loading plate and the limiting plate are automatically cleaned through the cleaning assembly, the cleaning efficiency is improved, the limiting plate is hinged to the sample loading plate, separate cleaning is facilitated, and cleaning is cleaner.

The application discloses a kind of transfer factor detection equipment and method, it is related to biological detection technical field, including microplate reader, detection chamber, microporous plate, detection component, the inner wall of the detection chamber is symmetrically provided with chute, and the inner wall of chute is connected with tray with sliding, the bottom of the tray is provided with push positioning mechanism, to in push detection, the detection position of the microporous plate is fixed with adhesion.This kind of transfer factor detection equipment, in push detection process, can be rotated by driving tray to drive gear, using the cooperation of guide groove and shaft, four positioning blocks are synchronously moved inward, so that four positioning blocks can be used to adhere positioning four sidewalls of microporous plate when being pushed to detection position, guarantee the accuracy of microporous plate position in detection process, in turn guarantee the accuracy of subsequent detection result of the equipment.

1. The name of the design product: multifunctional enzyme label instrument. 2. The use of the design product: for nucleic acid, protein concentration, enzyme activity analysis and other conventional molecular detection; signal transduction research, cell signal event active oxygen, modification detection; cell viability, apoptosis, killing and other overall level analysis. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.

The utility model belongs to the technical field of optical fiber assemblies, and particularly relates to an optical fiber assembly suitable for a microplate reader, which comprises a first optical fiber group and a second optical fiber group which need to be butted, and the first optical fiber group is sequentially provided with a first fiber core, a first sheath and a first joint from inside to outside; the second optical fiber group is sequentially provided with a second fiber core, a second sheath and a second connector from inside to outside, the first fiber core protrudes out of the front end of the first connector, and the exterior of the first connector is elastically connected with a floating sleeve matched with the protruding part of the first fiber core; it can be understood that the second fiber core is designed to be of an inward sinking structure, the first fiber core is designed to be of a protruding structure, the connecting face of the second fiber core and the first fiber core is arranged in the second connector to reduce the risk of light leakage, and the floating sleeve is additionally arranged outside the first fiber core to improve the protection performance; and meanwhile, the first magnetic blocks are inserted into the positioning grooves to prevent rotation and radial deviation, and the arc-shaped surface adsorption design of the first and second magnetic blocks is matched to increase the contact area, so that the suction force and the connection reliability are enhanced.

The invention develops a Brucella colorimetric detection method based on DNAWalker driving double signal amplification. The detection principle is as follows: target DNA triggers AP hybridization to form Y-type connection with CP, an Nt.BbvCI restriction enzyme cutting site is exposed, DNAWalker is released through restriction enzyme cutting circulation, and target circulation amplification is realized. Meanwhile, PDANS (palladium-iron-nano particles) is synthesized through Fe < 2 + > / H2O2 Fenton reaction, a pH indicator is loaded through silver nano particle modification (PDANS (at) Ag), and a signal is enhanced. An amplified product is combined with PDANS (at) Ag through a magnetic bead sandwich structure, remarkable color change is caused, and quantitative detection can be performed through a smart phone App (such as' Color Grab ') or a microwell plate reader. The method is easy and convenient to operate, high in sensitivity and suitable for on-site rapid identification of Brucella.

The invention relates to the technical field of microbial screening, in particular to a method for high-throughput screening of flavone-transformed bacteria and a screening system thereof. The method comprises the following steps: firstly, inoculating a to-be-detected strain into a basal culture medium containing a flavone substrate for fermentation, then scanning fermentation broth at two different time points by using a full-wavelength microplate reader through a quartz microplate, calculating an absorbance difference value at at least one preset wavelength, and finally, calculating the absorbance difference value at least one preset wavelength. The strains with the absorbance difference larger than a preset threshold value are screened as primary screening positive strains. Compared with the traditional technology, the method has the advantages that the screening efficiency is improved by hundreds of times, the sample consumption is reduced by 75%, the industrial bottleneck that multi-structure flavonoids need to be repeatedly developed is broken through, and an innovative tool is provided for microbial resource development.

The invention relates to an automatic multi-temperature-control microplate reader based on integration of a mechanical arm and artificial intelligence (AI), and aims to improve automation and accuracy of microbe growth monitoring and analysis. Operation such as culture dish taking and placing, culture solution adding and timing sampling can be automatically completed through the mechanical arm. The AI algorithm automatically adjusts parameters such as culture temperature, humidity, illumination and the like according to real-time data and environmental conditions so as to optimize the growth environment of microorganisms. Meanwhile, key indexes such as a light absorption value and cell density are monitored, a microorganism growth curve is drawn in combination with AI analysis, and important parameters such as the growth rate and the maximum growth amount are calculated. The AI can also mine potential laws in the data and provide valuable research information. The instrument improves the efficiency, accuracy and reliability of microbiological research, is widely applied to the fields of microbiological growth law research, resource development, disease diagnosis and the like, and promotes related technical progress.

The application discloses a high-throughput screening method of phenylethanolamine-N-methyltransferase. Substrate norepinephrine and S-adenosyl methionine, PBS buffer, S-adenosyl-L-homocysteine hydrolase SAHH are added in a 96-well plate of a microplate reader, enzyme sample phenylethanolamine-N-methyltransferase is added, in a microplate shaking reactor, 20-50 DEG C, 500-1000 rpm, reaction for 1-3 h, 4 DEG C, 3700 rpm centrifugation, the supernatant is taken, compound NDCC is added again, reaction for 0-90 min, during the reaction, the microplate is taken out every 1-30 min, and the microplate reader is used to detect the absorbance value; according to the absorbance value change, the enzyme activity is defined as the change of OD405nm absorbance value per minute * 1000000 under specific conditions. The application has the characteristics of simple operation, low cost and accurate detection, and has far-reaching significance for the directed evolution of phenylethanolamine-N-methyltransferase.

The invention provides a dynamic cell detection method based on super-resolution imaging and semi-quantification, and relates to the field of biomedical detection. The method comprises the following steps: constructing cell experiment groups and performing intervention; after dyeing by adopting a specific fluorescent probe, carrying out super-resolution imaging and image analysis by utilizing a structured light illumination microscope to obtain first semi-quantitative data; detecting by using a fluorescence microplate reader in parallel to obtain second data, and carrying out cross validation; and finally evaluating the drug function based on the valid data. According to the invention, the combination of subcellular morphology observation and multi-channel fluorescence semi-quantitative analysis is realized, and the accuracy and reliability of diabetes drug screening and cell function evaluation are remarkably improved.

The invention belongs to the technical field of rice variety screening, and particularly relates to a rice leaf color control gene ygl335 and a molecular marker and application thereof. By cloning the rice leaf color control gene and the encoded protein thereof, the invention reveals that the gene affects the synthesis of photosynthetic pigments by regulating chloroplast development for the first time, and provides a new leaf color gene resource for leisure sightseeing agriculture. According to the SNP molecular marker developed on the basis of the difference basic groups of ygl335 and wild type WT, a three-primer PCR-microplate reader method is combined, a mutant type, a wild type and a heterozygote can be rapidly and accurately distinguished, high-throughput genotype identification can be achieved without electrophoresis, the efficiency and convenience of molecular marker assisted breeding are remarkably improved, and the application prospect is wide. And an efficient tool is provided for early selection of rice leaf color characters. The application of the invention promotes the development of rice molecular design breeding, and has significant application prospects.

A temperature equalizing plate (1) for a micro plate reader (4), onto which temperature equalizing plate (1) a microplate with wells can be removably arranged, the temperature equalizing plate (1) having a plate-shaped body, wherein a first vertical offset is provided between four upward facing corner surfaces (100) and four upward facing edge surfaces (110) and wherein a second vertical offset is provided between the four upward facing edge surfaces (110) and one upward facing center surface (120), resulting in gaps between at least some of the upward facing surfaces (100;110;120) of the temperature equalizing plate (1) and corresponding wells of the thereon arranged microplate.

The invention provides a lung qi and blood barrier protection effect evaluation method. Specifically, the invention provides a method for evaluating the lung qi and blood barrier protection effect of a to-be-detected medicine, which comprises the following steps: (i) providing a pulmonary alveolar lavage fluid (BALF) sample of a subject receiving the to-be-detected medicine and / or reference substance administration; (ii) extracting a cell precipitate from the BALF and lysing the cell precipitate to obtain a protein extract; (iii) dyeing the protein extract and the protein standard substance with a Prussian blue dyeing solution; (iv) measuring the absorbance at the wavelength of 680 + / -10nm by using a microplate reader; wherein a standard curve is drawn according to the absorbance of the protein standard substances with different concentrations, and the absorbance of the protein extract is substituted into the standard curve to calculate the concentration of the ferrihemicin, so that the protective effect of the medicine on the lung qi and blood barrier is judged. The method disclosed by the invention can be used for simply, quickly and accurately evaluating the lung qi and blood barrier protection effect of the to-be-detected medicine.

The invention discloses a soil extracellular polymeric substance extraction and determination method based on a cation exchange resin method, and relates to the technical field of soil analysis, and the method specifically comprises the following steps: firstly, cleaning cation exchange resin with hot water, then sequentially adding HC l, NaC l and NaOH solutions, oscillating, and airing to obtain CER resin particles; the method comprises the following steps: treating a fresh soil sample by using a CaC12 solution and a buffer solution to obtain a pretreated soil sample; weighing CER resin particles, adding the CER resin particles into a centrifugal tube filled with the pretreated soil sample, oscillating, centrifuging and filtering to obtain an EPS extracting solution; and finally, detecting the absorbance of the EPS extracting solution under different wavelengths by using a microplate reader to respectively obtain the polysaccharide content and the protein content. According to the EPS extraction and determination method, the extraction efficiency of the EPS in the soil is effectively improved by optimizing the pretreatment steps of the cation exchange resin and accurately controlling parameter conditions such as the resin dosage, the extraction time and the rotating speed.

The invention relates to a microplate reader (1) comprising: a receiving device (2) for mechanically receiving a microplate (3) which has a plurality of recesses (4), in which recesses (4) samples to be analysed can be arranged, wherein the receiving device (2) is designed to be movable into an analysis position within a housing (13) of the microplate reader (1) and out of the housing (13) into a receiving position; and an optical detector (5) arranged within the housing (13) for detecting optical radiation at each individual recess (4) of the microplate (3) received in the receiving device (2), wherein the receiving device (2) and the optical detector (5) are arranged so as to be movable relative to one another in order to position the received microplate (3) relative to the optical detector (5) for successive measurements at different recesses (4). The microplate reader according to the invention is characterised in that at least one optical sensor (10), in particular a camera, is arranged or can be arranged on the housing (13) in such a manner that at least one partial region of the receiving device (2) and / or of the microplate (3) is detected, in particular via image capture, outside the housing (13), in particular in the receiving position, and / or in that the optical sensor (10) is designed to detect ambient conditions.

The invention belongs to the technical field of detection equipment, and provides chemical detection equipment for immune cells, which comprises a microplate reader body, a detection bin is arranged in the microplate reader body, a detection placement plate is arranged in the detection bin, a sealing door is arranged on the side surface of the detection bin, and a protection box surrounding the detection bin is fixedly mounted on the side surface of the microplate reader body. A cover plate is hinged to the side wall of the protection box. A frame-shaped installation frame is fixedly installed in the protection box, a base plate is fixedly installed at one end of the installation frame, a positioning plate is fixedly installed on the base plate, a side plate is fixedly installed on the side face of the positioning plate, and installation shafts are rotationally installed at the two ends of the base plate. Compared with the prior art, the device disclosed by the invention has the beneficial effects that rapid placement of the microwell plate is realized, the microwell plate is prevented from being held by a detector for a long time, collision between the microwell plate and the detection placement plate is avoided, and the placement stability of the microwell plate is improved.

The invention relates to a method for separating, measuring and collecting a density gradient ultracentrifugal sample. The method comprises the following steps: S1, preparing a density gradient solution by utilizing a full-automatic density gradiometer; s2, adding a to-be-detected sample to the top of the density gradient solution, and then carrying out overspeed refrigerated centrifugation; after ultracentrifugation is completed, all components in the sample are separated into a specific density layer; s3, separating and dropwise adding the centrifuged density gradient solution into a 96-hole ultraviolet permeable elisa plate by using a density gradient solution separation system according to a siphon principle, so that target components in the density gradient sample are accurately separated into specific sample holes; s4, putting the 96-hole ultraviolet permeable elisa plate into a full-wavelength microplate reader, reading and measuring an OD value, and determining target component sample holes; s5, calculating the sample concentration; and S6, selecting required sample holes, and collecting and separating the solution in the corresponding holes to obtain the purified and concentrated density gradient ultracentrifugation sample target component. The method is simple, easy to operate, accurate and reliable.

The invention discloses a rapid detection method for key flavor substances in Changsha stinky tofu brine, and relates to the technical field of analysis and testing. The method aims at solving the problems that an existing detection method is complex and high in cost and cannot quantify key flavor substances, specific color development systems are adopted for the four key flavor substances including phenol, 4-methylphenol, indole and 3-methylindole for color development reaction, absorbance data are obtained through ultraviolet-visible full-wavelength scanning of a microplate reader, the absorbance data are analyzed, and the quantitative analysis result is obtained. And calculating the content in combination with a partial least square model. The method is easy and convenient to operate, low in cost and high in accuracy, brine flavor substances can be rapidly detected, and a support is provided for Changsha stinky tofu brine quality control and industry standardization.

This invention discloses a rapid detection method for protein in feed based on wet chemistry. The method includes the following steps: (1) mixing the feed sample with the sample extraction buffer, shaking to extract, centrifuging, and taking the supernatant; (2) adding the working solution of the protein standard and the supernatant to the diquinoline formic acid working solution, incubating, and measuring the absorbance value at a wavelength of 562 nm using an ELISA reader to obtain the absorbance values ​​of the protein standard and the supernatant; plotting a standard curve with the concentration of the protein standard as the abscissa and the absorbance value as the ordinate, and obtaining a linear regression equation; substituting the absorbance value of the supernatant into the linear regression equation to calculate the protein concentration in the supernatant, and then calculating the protein content in the feed sample based on the extraction buffer volume in step (1). This invention achieves a high degree of consistency between the detection results and the Kjeldahl nitrogen determination method, while significantly shortening the detection time of a single sample to less than 1 hour.

The invention discloses a KASP molecular marker linked with a tobacco black shank resistance Ph introduction fragment and application of the KASP molecular marker. The invention discloses a KASP molecular marker linked with a tobacco black shank resistance Ph introduction fragment. The sequences of a primer pair of the KASP molecular marker are as shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The invention further discloses application of the KASP molecular marker linked with the tobacco black shank resistance Ph introduction fragment in screening of tobacco with black shank resistance or application in breeding of tobacco varieties with black shank resistance. According to the KASP molecular marker, the simplicity of conventional PCR and the high sensitivity of fluorescence detection are fused, the experiment cost is greatly reduced, and the detection flux is improved. According to the method disclosed by the invention, only a small-scale breeding laboratory is usually provided with necessary instruments and equipment, electrophoretic analysis is not needed any more, and sample detection can be completed on a microplate reader or a quantitative PCR instrument by utilizing a 96-pore plate or a 384-pore plate.