Novel coronavirus rapid detection test paper and detection method

Abstract

The invention discloses novel coronavirus rapid detection test paper and a detection method. The novel coronavirus rapid detection test paper is composed of a sample pad, a colloidal gold pad, a reaction color development area, a water absorption area and a supporting plate; the sample pad and the water absorption pad are arranged on the supporting plate and located on the two opposite sides of the supporting plate respectively, and the sample pad is suction filter paper or a glass fiber membrane and used for bearing an object to be detected; a nitrocellulose membrane is fixed on the supporting plate, and the absorbent pad is absorbent paper; through double binding of a novel coronavirus antibody and a receptor ACE2 protein of a virus, a coronavirus protein antigen is specifically capturedand bound by adopting double enzyme-linked immunosorbent assay; the ACE2 antibody in the test paper reaction color development area is combined with biotin, avidin is connected with horse radish peroxidase, and through a biotin-avidin system, rapid judgment can be achieved through chemical color development, and the virus content can also be accurately detected through a microplate reader.

Description

technical field [0001] The invention relates to the technical field of rapid detection reagents for novel coronavirus antigens, in particular to a rapid detection test strip and a detection method for novel coronavirus antigens, which can detect human whole blood, serum, plasma, saliva, throat swabs, feces, urine and other samples . Background technique [0002] Due to limited materials and detection methods, it is known that the qPCR method is required to diagnose the new coronavirus, which is difficult to operate and relatively expensive compared with other detection methods. As a result, the number of existing test strips cannot meet the unified detection of a large number of citizens. At present, the number of suspected cases of new coronavirus remains high. At present, early diagnosis of viral infection has important clinical value. [0003] By double-binding novel coronavirus (2019-nCoV) IgM and IgG antibodies and the receptor ACE2 protein of the virus, "double e...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a rapid detection of novel coronavirus detection test paper and detection method to solve the problems of difficult operation and high cost compared with other detection methods proposed in the above-mentioned background technology; the detection method obtains the common ACE2 protein and the new coronavirus antibody IgM and IgG are used to detect the new coronavirus by "double enzyme-linked immunosorbent assay". This product uses antibodies and receptor proteins that can specifically react with the coronavirus. The receptor protein is immobilized in nitric acid On the cellulose membrane, another antibody is combined with colloidal gold. If there is a new type of coronavirus CV in the sample to be tested, the CV antigen first reacts with the anti-CV antibody colloidal gold conjugate, moves on the membrane through capillary phenomenon, and finally binds to the immobilized Receptor proteins on the NC membrane bi

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