The invention discloses a novel
fluorescence immunochromatography test strip for joint inspection of SARS-CoV-2 IgG-IgM antibodies of coronaviruses. The test strip comprises a bottom plate, a sample pad, a combination pad, a
nitrocellulose membrane and a water absorption pad, wherein the sample pad, the combination pad, the
nitrocellulose membrane and the water absorption pad are sequentially connected end to end and adhered to the bottom plate; the combination pad is coated with an SARS-CoV-2
structural protein-marker and a goat anti-rabbit IgG-marker, and the
nitrocellulose membrane is provided with a detection line T1 coated with a mouse anti-human
IgG monoclonal antibody, a detection line T2 coated with a mouse anti-human
IgM monoclonal antibody and a
quality control line C coated withrabbit IgG. When the test strip is used for quantitatively detecting SARS-CoV-2 IgG and IgM antibodies, the detection sensitivity is high, and the specificity is good and can reach 96%; the batch-to-batch difference is small, and good
repeatability is achieved; the test strip can be stored for half a year at normal temperature without reducing the sensitivity and has good stability; the test strip is simple to operate and low in cost, can quickly and quantitatively detect the levels of SARS-CoV-2 IgG and IgM antibodies in a
human body, assists a
nucleic acid detection means, and provides powerful support for epidemic situations.