Neutralizing antibody of coronavirus or antigen-binding fragment thereof

A technology that combines fragments and coronaviruses, applied in the direction of viruses/phages, antibodies, antiviral agents, etc., can solve problems such as loss of protective effect of mutant viruses

Active Publication Date: 2021-01-05
超非凡(上海)医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the non-conserved region recognized by these antibodies is mutated to produce a new circulating strain, the antibody will lose its protective effect against the mutant virus

Method used

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  • Neutralizing antibody of coronavirus or antigen-binding fragment thereof
  • Neutralizing antibody of coronavirus or antigen-binding fragment thereof
  • Neutralizing antibody of coronavirus or antigen-binding fragment thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1: Screening and detection of neutralizing antibodies to coronavirus

[0075] The inventor conducted a pseudovirus assay on the plasma of patients with novel coronavirus pneumonia (followed up two weeks after recovery and discharge) admitted to the inventor's unit (Shanghai Public Health Clinical Center) from January 20, 2020 to February 26, 2020. And experimental screening, it was found that the serum of three mild patients had strong neutralizing activity against the pseudovirus of SARS-CoV-2. With the written consent of the ethics committee of the inventor's unit and the patient himself, the peripheral blood was drawn for research.

[0076] 1. Sorting of peripheral blood memory B cells

[0077] 1) Separation of peripheral blood lymphocytes: extract the convalescent peripheral blood of the above-mentioned patients and mix it with an equal amount of normal saline, then use Lymphoprep (Stemcell Technologies, Cat. No. 07851) to separate peripheral blood lymphocyt...

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PUM

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Abstract

The invention relates to a neutralizing antibody of coronavirus or an antigen-binding fragment thereof, a nucleic acid molecule encoding the antibody or the antigen-binding fragment thereof, a vectorcontaining the nucleic acid molecule, a host cell containing the vector, application of the antibody or the antigen-binding fragment thereof in preparation of drugs for treating or preventing diseasescaused by coronavirus, and application in detection of products. The coronavirus antibody and the antigen-binding fragment thereof have broad-spectrum and strong neutralizing ability for various coronaviruses including SARS-CoV-2, and have good clinical application prospects in the future.

Description

technical field [0001] The present invention relates to a coronavirus neutralizing antibody or an antigen-binding fragment thereof, a nucleic acid molecule encoding the antibody or an antigen-binding fragment thereof, a vector comprising the nucleic acid molecule, a host cell comprising the vector, and the antibody or an antigen-binding fragment thereof The application of the fragments in the preparation of drugs for the treatment or prevention of diseases caused by coronaviruses, as well as the application in the detection of products, belongs to the field of biomedicine. Background technique [0002] Novel coronavirus pneumonia (2019-nCOV) is an acute respiratory infectious disease caused by a new type of coronavirus, SARS-COV-2. The virus is highly transmissible and can be transmitted through the respiratory tract, contact and other channels. Since the outbreak in December 2019, it has spread to all parts of the world, forming a worldwide pandemic. As of July 1, 2020, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C12N15/13G01N33/569G01N33/577A61K39/42A61P31/14
CPCC07K16/10G01N33/56983G01N33/577A61P31/14C07K2317/565C07K2317/56C07K2317/76C07K2317/92C12N2800/107G01N2333/165G01N2469/10A61K2039/505
Inventor 黄竞荷吴凡刘梅
Owner 超非凡(上海)医疗科技有限公司
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