827 results about "Peripheral blood" patented technology

The invention relates to the technical field of biology, in particular to a Treg cell amplification culture medium and a preparation method of Treg cells. According to the method for in-vitro amplification of the regulatory T cells (Treg) from peripheral blood, the amplification efficiency and purity of the Treg cells are improved, the culture cost is reduced, the culture period is shortened, and the problem of trophoblast cell residues in subsequent clinical application is solved.

The invention relates to the technical field of medical monitoring and intelligent decision making, and particularly discloses an intraoperative noninvasive real-time blood flow monitoring and medication intervention analysis system. Pulse wave oscillation harmonic component characteristics and diastolic attenuation characteristic characteristics are extracted by using variational mode decomposition and a polynomial fitting algorithm; the multi-dimensional features are fused and then input into a deep learning classification model, and accurate recognition of insufficient heart pump efficiency, abnormal peripheral vascular tension and circulation capacity imbalance states is achieved; time sequence prediction and grading early warning are carried out based on the hidden Markov model and the individualized baseline safety interval, and finally a targeted medication scheme is output through the intervention strategy knowledge base.

The invention discloses a diabetes cognitive impairment method based on metabonomics analysis and prediction, and relates to the technical field of biological information, and the method comprises the following steps: S1, obtaining metabonomics data and immunomics data from a peripheral blood sample of a diabetic patient, extracting relevant time sequence data aiming at glucose metabolism, and calculating the glucose metabolism related time sequence data; processing the sequence data by adopting a time sequence analysis algorithm to obtain time sequence change characteristics; s2, constructing a cross-omics interaction network according to time sequence change characteristics, integrating an incidence relation between metabolite concentration and immune factor expression, and setting a dynamic interaction mode; according to the diabetes cognitive impairment method based on metabonomics analysis and prediction, through multi-omics data integration and dynamic network analysis, the precision and reliability of diabetes cognitive impairment mechanism analysis are remarkably improved, and a theoretical basis is provided for precise intervention.

The invention discloses a human body peripheral blood vessel photoacoustic three-dimensional imaging device and an imaging method thereof, and belongs to the field of blood vessel imaging. Through high resolution and functional monitoring capability of photoacoustic imaging, detailed blood vessel structure and blood flow dynamic information are provided, and early recognition and treatment effect evaluation of common lesions such as diabetic foot and peripheral artery diseases are promoted; meanwhile, a mechanical system is adopted, so that the detection front end with relatively high quality can be borne, and stable operation is realized, and a relatively good photoacoustic imaging result is obtained; the system has a highly stable accurate scanning imaging mode, and is convenient for multiple imaging modes of clinical application; through the arrangement of the imaging water tank and the lifting structure of the imaging water tank, switching of imaging modes in a limited space can be ensured, so that the device can be suitable for the layout of a portal type three-dimensional moving device. The system is expected to promote the portability of equipment, reduce the research and development cost of the equipment, be widely applied to clinical practice and community medical treatment, and improve the disease management level.

The invention provides an intraoperative path optimization method and system based on solid tumor preoperative image analysis, and the method comprises the steps: constructing a three-dimensional model of a tumor and peripheral blood vessels and nerves according to a preoperative image, and carrying out the dissection risk marking of the three-dimensional model through a deep learning model, generating an anatomical risk image with risk gradient value distribution; taking the minimum intraoperative bleeding amount and the highest tumor excision rate as a reward function, combining the three-dimensional model, an anatomical risk image and historical similar case data, and simulating multiple operation paths based on an adversarial reinforcement learning algorithm to obtain an optimal operation path; the method comprises the following steps: acquiring the pose, ultrasonic image and fluorescence image of a surgical instrument in real time during surgery, matching the preoperative image with the intraoperative image based on a biomechanical model driven non-rigid registration algorithm, predicting organ displacement, and correcting an optimal surgical path, thereby avoiding accidental injury of key tissues, ensuring that an infiltration area is brought into the surgical path in time, and improving the surgical efficiency. Residues are reduced, and static planning failure is avoided.

The invention relates to the technical field of biomedicine, in particular to a method for constructing a plasma ctDNA organ distribution characteristic spectrum of advanced colorectal cancer. The method comprises the following steps: collecting a peripheral blood sample at multiple time points, separating plasma by adopting a double-centrifugal method, and extracting circulating tumor DNA (Deoxyribose Nucleic Acid); carrying out whole exome sequencing based on ctDNA to obtain genome variation information and calculating variation allele frequency, and synchronously detecting the expression quantity of immune-related proteins by adopting an Olink proteomics technology; integrating the genome data, the protein expression data and the clinical pathological features, and constructing a multi-dimensional feature data matrix; and taking the organ metastasis condition confirmed by iconography as a supervision label, training a model by applying a machine learning algorithm, screening key prediction factors, constructing a quantitative prediction model, and finally generating a visual organ metastasis tendency prediction map. According to the method, early and accurate prediction of the advanced colorectal cancer organ metastasis tendency is realized through multi-omics data collaborative analysis and machine learning modeling.

The invention belongs to the field of biological medicine, and discloses a blood marker for identifying epilepsy convulsion and application thereof. The inventor finds that the LRG1 is obviously increased during epileptic convulsion, which indicates that the increase of the blood LRG1 is a blood marker during the acute attack stage of epileptic convulsion. The invention proposes and verifies that the LRG1 is the peripheral blood marker in the acute attack stage of epilepsy convulsion for the first time, and can be used as a clinical auxiliary diagnosis and treatment target.

The invention discloses an auxiliary diagnosis system for renal transplantation rejection and typing thereof based on a peripheral blood immune cell map. Comprising a peripheral blood single cell acquisition unit, a mass spectrum flow cytometry unit, an original data preprocessing unit, a data conversion and normalization processing unit, a peripheral blood immune cell grouping acquisition unit, a peripheral blood immune cell map construction unit, a feature vector acquisition unit, a rejection prediction unit and a rejection typing prediction unit. According to the invention, a kidney transplantation two-stage diagnosis architecture is created for the first time; rejection detection and typing identification are realized through classification of an independently trained random forest binary classifier, and different TCMR / ABMR immune mechanisms are accurately matched; the traditional biopsy limitation is broken through, an auxiliary diagnosis scheme with high precision, low damage, moderate cost and typing identification capability is provided for the kidney transplantation rejection, and the method has important significance on timely discovery of the rejection of a kidney transplantation patient and improvement of prognosis.

The embodiment of the invention relates to the technical field of drug detection, in particular to an anti-CD38 antibody drug receptor occupation detection method and system, a peripheral blood sample is used for preparing a single-cell suspension, the single-cell suspension is divided into a TEST tube and an FMO tube, a cell surface antibody and an RO detection antibody are added into the TEST tube, only the cell surface antibody is added into the FMO tube, and only the RO detection antibody is added into the FMO tube. The TEST tube and the FMO tube are subjected to sample detection analysis, so that the average fluorescence intensity of RO detection antibodies of the TEST tube and the FMO tube is obtained. And performing RO detection analysis by adopting a dissociation method, taking the tubes which are not combined with the medicine as a total receptor group, taking the tubes which are combined with the medicine as a dissociation receptor group, and calculating an RO detection result according to the average fluorescence intensity of the TEST tube and the FMO tube.

The invention relates to a training method of a respiratory tract infection disease progress and prognosis prediction model. The training method comprises the following steps: extracting mRNA from peripheral blood of a target patient, and carrying out transcriptome sequencing to obtain a sequencing result; based on the ferroptosis related gene set, comparing ferroptosis score differences of two groups of patients with community-acquired pneumonia and sepsis, and screening corresponding ferroptosis related genes with statistical significance from a sequencing result; screening out genes meeting preset conditions from the ferroptosis related genes based on LASSO regression; and establishing an RTI clinical outcome prediction model through logistic regression by taking whether the patient is sepsis or not as an outcome dichotomy variable and taking the screened gene expression quantity as a prediction variable. According to the invention, after the prediction model is subjected to machine learning screening such as LASSO and the like, the core feature with the highest prediction value is reserved, so that the risk of over-fitting of the model on training data is reduced.

The invention discloses an ALS prognosis evaluation system based on UBE2J2 gene methylation and expression level, and relates to the technical field of biomedicine and bioinformatics. Comprising a data acquisition module, a methylation detection module, an expression quantity determination module, a data preprocessing module, a preliminary survival analysis module, a multivariable Cox proportional risk model construction module, an individualized risk index calculation and grouping module and a visualization and clinical application module. By combining UBE2J2 gene methylation and expression quantity analysis, peripheral blood non-invasive detection, standardized experiment and computational analysis process and visual presentation, the method is technically and remarkably superior to the prior art, high accuracy, operability, repeatability and expandability are realized, and the method is suitable for large-scale popularization and application. And the technical defects of single molecular marker, complicated operation, inaccurate prediction and the like in ALS prognosis evaluation in the prior art are overcome.

The invention belongs to the field of biological medicine industry, and discloses an acute pyelonephritis peripheral blood marker. The inventor finds and verifies that the average value of LRG1 in blood is higher than that of a normal person during acute pyelonephritis for the first time, LRG1 serving as a peripheral blood marker of acute pyelonephritis has high sensitivity and specificity, and treatment of acute pyelonephritis can be guided more effectively.

The invention belongs to the technical field of biological intelligent detection, and particularly relates to a circulating tumor cell automatic detection device based on a micro-fluidic nano chip and AI analysis and application. The device comprises a micro-fluidic chip module, an optical acquisition module, an image recognition module, a feedback control module and a liquid path control module, and can realize efficient enrichment, image recognition and dynamic fluidic regulation of circulating tumor cells in peripheral blood. By constructing a change trend model of an image frame sequence, cell state changes are recognized in real time, flow velocity and channel control instructions are output, and collaborative optimization of recognition precision and the sample utilization rate is achieved. The device is suitable for tumor early screening, curative effect monitoring and recurrence early warning scenes.

The invention relates to an application of a small molecule medicine STK000311 in a medicine for treating a cGAS-STING pathway abnormal activation disease, and belongs to the technical field of biological medicines. According to the application disclosed by the invention, the small-molecular medicine STK000311 or the pharmaceutically acceptable salt of the small-molecular medicine STK000311 serving as the cGAS-STING signaling pathway inhibitor has an obvious treatment effect on the cGAS-STING pathway abnormal activation diseases for the first time, and verification experiments show that the small-molecular medicine STK000311 can be used for obviously inhibiting the activity of human cGAS protein in a targeted manner and inhibiting cGAS-mediated antiviral immune response; the transcription level of ISGs in PBMCs (peripheral blood mononuclear cells) of an SLE (systemic lupus erythematosus) patient is inhibited, the fact that the small-molecule medicine STK000311 has huge potential of developing cGAS activity related diseases is fully proved, a novel treatment medicine is provided for clinic, and the small-molecule medicine STK000311 has good clinical application value and wide application prospect.

The invention belongs to the field of biological medicine industry, and discloses a peripheral blood marker and system for identifying the severity of viral myocarditis or coronary artery stenosis. The inventor finds for the first time that the average value of LRG1 in blood is not obviously changed compared with that of a normal person when mild and moderate myocarditis and microcirculation angina pectoris with normal coronary artery or coronary artery stenosis smaller than 10% exist, and the blood LRG1 is obviously increased when the coronary artery stenosis of the coronary heart disease exceeds 70%; when severe myocarditis occurs, the blood LRG1 is also obviously increased, so that the increase of the blood LRG1 is a blood marker for patients with severe myocarditis or severe coronary artery lesion, and the blank that blood indexes are lacked in the prior art to prompt the patients to have severe coronary artery stenosis is filled. By detecting the LRG1 content of the peripheral blood and combining with other conventional examinations, the severity of myocarditis or coronary stenosis can be simply, accurately and quickly distinguished, and a corresponding basis is provided for subsequent accurate treatment.

The present disclosure provides a method for obtaining a cancer risk prediction marker, a cancer risk assessment method, an electronic device, and a storage medium performed by a machine. The method for obtaining a cancer risk prediction marker includes: analyzing a plurality of peripheral blood circulating cell-free DNA (cfDNA) fragments of a biological sample of a subject to obtain sequence reads; obtaining data of a distribution of a nucleosome, and determining a predetermined interval upstream and downstream of a nucleosome center position as a nucleosome protection region; screening sequence reads of partial cfDNA fragments whose ends are located in the nucleosome protection region from the sequence reads of the plurality of cfDNA fragments; calculating a first fragmentation feature in the sequence reads of the plurality of cfDNA fragments, and calculating a second fragmentation feature in the sequence reads of the screened partial cfDNA fragments; and calculating one or more difference values of a change level of the second fragmentation feature relative to the first fragmentation feature as a cancer risk prediction marker.

The invention discloses a primer for diagnosis of early pregnancy of sows. The nucleotide sequence of the primer is shown as follows: SRY-O-F: 5 '-CAGCAAAATATTCTCGCCTTGG-3', and the nucleotide sequence of the primer is shown as follows. And SRY-O-R: 5 '-CATCCTCTCTCTACGC-3', and SRY-O-R: 5 '- SRY-I-F: 5 '-ATATTCTCGCCTTGGGG-3', SRY-I-F: 5 '- And SRY-I-R is 5 '-GCTTTCGGCTTCTGTA-3', and the formula is shown in the description. Two pairs of primers with high sensitivity and strong specificity are designed through comparative analysis of pig SRY gene sequences, fetal free DNA in peripheral blood of sows is used as a template, and the specificity and sensitivity of PCR are enhanced through amplification of the two pairs of primers. Through two rounds of PCR amplification, whether the sow is pregnant or not can be detected on the 18th day of pregnancy, and the accuracy of the detection result is extremely high and reaches 100%. Compared with the prior art that the ZFY gene is used for detecting the sows on the twentieth breeding day, the method has the advantages that the detection time can be advanced to the eighteenth breeding day, and the detection accuracy is greatly improved (100% vs 10%). According to the method, powerful technical support is provided for early and accurate identification of the non-pregnant sows, reduction of non-production days and feeding cost and smooth execution of a production plan.

Perfusion systems and methods are provided for enhancing perfusion to the vasculature of a patient suffering from reduced blood flow. For example, the system can increase peripheral blood flow to reduce limb ischemia, in which an extracorporeal pump having a controller, and catheter / tubing set, employed alone or in conjunction with an interventional or circulatory assist device, withdraws blood from a patient's vasculature and reintroduces that blood at another location within the patient's vasculature at a controlled local pressure or flow rate, without interfering with operation of the interventional or circulatory assist device or surgical intervention.

The invention discloses an early-stage lung cancer prediction method based on a multi-modal eccDNA marker, and relates to the technical field of liquid biopsy, and the method comprises the following steps: collecting a peripheral blood sample, carrying out differential centrifugal separation on plasma, constructing a cfDNA library, and carrying out double-end sequencing to obtain original sequencing data; extracting structural features through an eccDNA model, and inputting the structural features into a first machine learning model to generate a circular DNA score; extracting variation features through an SNV model, and inputting the variation features into a second machine learning model to generate a spectrum feature score; extracting copy number features through a CNV model, and inputting the copy number features into a third machine learning model to generate copy number scores; carrying out probability distribution calibration on the circular DNA score, the spectrum feature score and the copy number score; inputting the three types of molecular features into a deep neural network to generate a first fusion score; inputting the calibrated score into a logistic regression model to generate a second fusion score; and generating a final lung cancer risk probability according to the first fusion score and the second fusion score.

Disclosed is a system and processor-implemented method of estimating one or more vital signs of a user based on a video of the user by using artificial intelligence, comprising extracting a plurality of face frames and one or more time stamps from the video of the user; determining at least one region of interest (ROI) in the plurality of face frames using a histogram of oriented gradients (HOG); extracting a plurality of health indicator signals by detecting volumetric changes in a peripheral blood circulation in the at least one ROI based on a remote photoplethysmography (RPPG); and estimating the one or more vital signs of the user based on the plurality of health indicator signals and the one or more time stamps.

The peripheral blood taking needle subpackaging equipment comprises a machine table, and a blood taking needle feeding mechanism, a blood taking needle conveying and transferring mechanism, a paper box feeding and forming mechanism, a paper box conveying belt, a blood taking needle boxing mechanism and a box covering and discharging mechanism are arranged on the machine table; the blood taking needle feeding mechanism is used for conveying blood taking needles to the blood taking needle conveying and transferring mechanism. The blood taking needle conveying and transferring mechanism is used for conveying and transferring the blood taking needles to the blood taking needle boxing mechanism; the paper box feeding and forming mechanism is used for folding and forming paper boxes and placing the paper boxes on the paper box conveying belt. The paper box conveying belt is connected with the paper box feeding and forming mechanism, the blood taking needle boxing mechanism and the box covering and discharging mechanism. The blood taking needle boxing mechanism is used for feeding the blood taking needles into the paper box, and the box covering and discharging mechanism is used for covering the paper box and discharging the blood taking needles out of the machine table. According to the full-automatic blood taking needle subpackaging machine, the whole process from feeding to boxing, box covering and discharging of blood taking needles can be automated, and the subpackaging efficiency of the blood taking needles is remarkably improved; manual participation is reduced, and the labor cost can also be reduced.

The invention relates to a construction method of a nanometer antibody coding DNA library. The method comprises the following steps: screening animal peripheral blood mononuclear cells (PBMC) by using immunogen to obtain positive cells specifically bound with the immunogen; and amplifying a nucleic acid sample derived from the positive cells so as to obtain a coding DNA library of the nano-antibody, wherein the nano antibody is a nano antibody specifically binding to the immunogen, and wherein the amplification comprises the use of one or more groups of primer pairs, each group of primer pairs of the one or more groups of primer pairs comprising one or more forward primers and one or more reverse primers. A large number of diversified DNA sequences covering a large number of low-abundance sequences are obtained. According to the technical route for rapidly preparing the VHH antibody based on high-throughput sequencing, the high-affinity and high-specificity VHH antibody can be efficiently and rapidly obtained, and the raw material requirement of a high-quality diagnostic reagent is met.

This utility model relates to an innovation of a carotid artery vasodilator that can be used at home and in the emergency room as a supplement to drug treatment for conditions such as carotid artery obstruction in the neck region. It increases the production of nitric oxide mediated by endothelial nitric oxide synthase in the intact endothelial layer of the carotid artery, which is located on the inner surface of the neck brace. It can be applied for a certain period of time for each patient. The intermittent or continuous operation function can be selected through an adjustable power circuit. It only affects the lesion area and does not affect the patient's peripheral blood vessels. It can be used in situations where nitrates may be prohibited. It prevents the reduction of blood flow to the brain tissue that may occur due to jugular venous constriction. The flexible structure of the applicator neck brace (9) can be maintained in the application area for a long time and includes a pulsed magnetic field (PMF).

Exemplary embodiments described herein are directed to a vascular access catheter device with an inline needle, the device having a needle portion that is linearly displaceable relative to a catheter portion, and vice versa, and to methods of performing blood transfer procedures using said device. Retraction of the needle portion relative to the catheter portion allows the catheter of the catheter portion to cover the needle tip during a blood transfer procedure, thereby preventing needle damage to a fistula / graft or peripheral vessel of a patient, as well as possible needle stick injuries to health care workers during removal / disposal of a used device. Because the needle still remains largely within the catheter after retraction, there is no risk of catheter collapse, kinking, etc., during use, which could compromise blood flow during a hemodialysis or other vascular access procedure.

Disclosed are methods of isolating T cells and TCRs having antigenic specificity for a mutated amino acid sequence encoded by a cancer-specific mutation. Also disclosed are related methods of preparing a population of cells, populations of cells, TCRs, pharmaceutical compositions, and methods of treating or preventing cancer.

The application provides a kind of HLA-A24:02 APC cell preparation method and application, it is related to cell preparation technical field.The present application constructs the APC cell (K562-HLA-A24:02) that can replace HLA-A24:02 subtype DC cell, fills the blank of HLA-A24:02 subtype special engineering APC cell, and the cell can realize the antigen presentation function consistent with natural HLA-A24:02 subtype DC cell, provides special tool cell for the immune research for the HLA subtype;The present application constructs APC cell with K562 cell line as base, K562 cell can be in vitro permanent passage amplification, without repeatedly separating and inducing DC cell from human peripheral blood, reduces the use amount of peripheral blood, reduces the raw material dependence and cost of cell acquisition, while avoiding the influence of individual difference of peripheral blood source on experimental results.

The invention discloses a peripheral vascular punctiform stent system comprising a punctiform stent unit and a delivery assembly unit. The dotted stent unit comprises a plurality of mutually independent dotted stents; the point-shaped supports are sequentially arranged in the length direction of the conveying assembly units. A radial supporting force range associated with the compression ratio is preset for each point-shaped stent unit; when the value of the compression ratio is 10%-55%, the value domain of the radial supporting force of the dotted stent units is 0.15-0.5 N / mm, and the value of the radial supporting force of any dotted stent does not exceed the endpoint value of the value domain of the radial supporting force of the dotted stent units. The radial supporting force range is designed in advance, so that the dotted stent units have redundancy, the stability defect of the dotted stent units after being unfolded in the blood vessel is overcome, unexpected displacement and restenosis of one or more dotted stent units are inhibited, and the original position is maintained for a long time.

The invention provides a pressure adjusting system and a method for adjusting the pressure of fluid in an inner cavity of medical equipment, and relates to a method for controlling a pressure control pump to enable the pressure of the fluid in the inner cavity of the medical equipment to change within a period of time. A coronary artery blood vessel remodeling catheter, a peripheral balloon dilatation catheter, a peripheral blood vessel remodeling catheter, an intracranial laser balloon dilatation catheter, a drug coating balloon dilatation catheter, a photodynamic balloon catheter, a mastoid balloon catheter, a constraint type balloon dilatation catheter and a spinous process balloon can be made. Expansion and contraction of a balloon body in a balloon catheter such as a drug delivery balloon catheter are regularly changed within a period of time, and therefore the treatment effect is better achieved.

The invention relates to the technical field of molecular biology, in particular to a methylation marker for diagnosis of diabetic nephropathy. Specifically, the methylation marker comprises a P site of a CAT gene promoter region and / or an E site of a CAT gene exon 1 region. Based on a detection system combining methylation sensitive restriction enzyme with quantitative PCR, the kit has the advantages of simplicity and convenience in operation, good repeatability, accuracy in quantification and easiness in standardization, and is suitable for low-invasive samples such as peripheral blood. By effectively distinguishing pure type 2 diabetes patients from diabetic nephropathy combined patients, a reliable technical means is provided for risk stratification, early intervention and dynamic monitoring of diabetic people, and the method has good clinical application prospects and popularization value.

A method is provided for recruiting hematopoietic progenitor cells from the bone marrow of a target to the peripheral blood. The method comprises (a) administering an effective dose of natalizumab to the target, and (b) administering an effective dose of a CXCR4 inhibitor to the target, at least 48 hours after step (a). The use of such recruitment procedures in cell collection and therapy is also provided.