331 results about "Metabolic Process" patented technology

Methods are provided for modulating the expression of genes involved in lipid metabolism, useful in the treatment of conditions associated with cardiovascular risk. Antisense oligonucleotides targeted to apolipoprotein B reduce the level of apolipoprotein B mRNA, lower serum cholesterol and shift liver gene expression profiles from those of an obese animal towards those of a lean animal. Further provided are methods for improving the cardiovascular risk of a subject through antisense inhibition of apolipoprotein B. Also provided are methods for employing antisense oligonucleotides targeted to apolipoprotein B to modulate a cellular pathway or metabolic process.

The invention relates to of medicine and medicine equipment and concerns techniques and means for medicinal action with electromagnetic fields to living tissues. A method provides a stimulating effect of an electromagnetic field to metabolic processes in tissues of an organism, that result in positive shifts of a functional state of regulatory systems. On a contactless electromagnetic action to members of a vascular system, biomechanical, bioelectrical and bioresonant processes in the vascular system control the tone of a vascular wall with a high degree of selectivity. The method and device allow to reduce treatment periods, post-operative and other types of rehabilitation due to a deep contactless interaction of biological tissues of a patient, provided by sensor fields of the organism at an information level, with low-frequency electromagnetic fields that are created by the inventive device with special bioadequate parameters relative to dynamic (that is, time and shape parameters), amplitude and geometric-spatial properties. The inventive device consists of a specialized biosynchronized generator of low-frequency electrical signals that arrive at an electromagnetic inductor converting the electrical signals into electromagnetic fields having given electromagnetic properties determined as a result of fundamental theoretical studies and experiments.

The methods described herein enable the evaluation of compounds on subjects to assess their therapeutic efficacy or toxic effects. The target of analysis is the underlying biochemical process or processes (i.e., metabolic process) thought to be involved in disease pathogenesis. Molecular flux rates within the one or more biochemical processes serve as biomarkers and are quantitated and compared with the molecular flux rates (i.e., biomarker) from control subjects (i.e., subjects not exposed to the compounds). Any change in the biomarker in the subject relative to the biomarker in the control subject provides the necessary information to evaluate therapeutic efficacy of an administered drug or a toxic effect and to develop the compound further if desired. In one aspect of the invention, stable isotope-labeled substrate molecules are administered to a subject and the label is incorporated into targeted molecules in a manner that reveals molecular flux rates through one or more metabolic pathways of interest. By this method, a comparison between subjects and control subjects reveals the effects of the chemical entity or entities on the biomarkers. This, in turn, allows for the identification of potential therapeutic uses or toxicities of the compound. Combinations of compounds can also be systematically evaluated for complementary, synergistic, or antagonistic actions on the metabolic pathways of interest, using the methods of the present invention as a strategy for identifying and confirming novel therapeutic or toxic combinations of compounds.

The invention discloses a method for transforming rice into fragrant rice rapidly. By means of the CRISPR / Case technology and related gene sequences in the rice fragrance metabolic process, a carrier is designed at the specific site, the element is transferred to non-fragrant rice through agrobacterium-mediated transformation, the gene is made to cause deletion mutation at the position of a fixed point, the gene is silent, fragrance cannot be normally metabolized and greatly accumulated, common rice is transformed into fragrant rice, then genetic transformation gene segments are separated from edited genes through selfing or hybridization, and the fragrant rice with the isozygoty capable of being stably inherited is rapidly obtained.

A method for increasing the target-specific toxicity of a drug is effected by pretargeting an enzyme to a mammalian target site, and then administering a cytotoxic drug known to act at the target site or a prodrug form thereof which is converted to the drug in situ, which drug is also detoxified to form an intermediate of lower toxicity using said mammal's ordinary metabolic processes, whereby the detoxified intermediate is reconverted to its more toxic form by the pretargeted enzyme and thus has enhanced cytotoxicity at the target site. Further enhancement can be achieved by pretargeting an enzyme which converts the prodrug to the cytotoxic drug at the target site. Kits for use with the method also are provided. The method and kits permit lower doses of cytotoxic agents, maximize target site activity and minimize systemic side effects.

The metabolic processes involved in the formation of any glucose-based metabolite of a metabolic network are determined. A precursor molecule is labeled with a stable carbon (13C) isotope at specific positions. The label is allowed to distribute and rearrange in the system. Metabolites are recovered and analyzed against a control system to determine a set of metabolic pathway substrate fluxes caused by changes to the test system relative to the control system such as the addition of compound being tested as a potential drug.

A method of doing business is disclosed whereby the process for selecting drug candidates is improved. The method involves the application of a technology which makes it possible to determine metabolic processes involved in the formation of any glucose-based metabolite. A precursor molecule is labeled with a stable carbon (13C) isotope at specific positions. The label is allowed to distribute and rearrange in the system. Metabolites are recovered and analyzed against a control system or known biochemical reactions and / or cycles to determine information such as metabolic pathway substrate flux caused by a compound acting on the system.

The methods of the present invention allow for the measurement of ribonucleotide reductase (RR) activity, an important enzyme in the de novo DNA synthesis pathway. Ribonucleotide reductase converts all four ribonucleotides to their deoxy form and is a rate-controlling step in this pathway. Biosynthetic pathways of deoxyribonucleotides (dN) have received considerable attention in the context of anti-proliferative chemotherapy. Inhibitors of various steps in dN biosynthesis, including inhibitors of RR are among the most useful chemotherapeutic agents in cancer, viral infections, and other therapeutic uses. DNA synthesis from the dN salvage pathway is also an important component to DNA replication. The relative contributions from RR vs. salvage pathways are critical to the actions and effectiveness of chemotherapeutic agents that act on nucleoside metabolic pathways. Until now, however, it has not been possible to study these metabolic processes in vivo. Disclosed within are methods of measuring RR activity in vivo and in vitro which find use, among other things, in drug discovery, development, and approval.

The methods described herein enable the evaluation of compounds on subjects to assess their therapeutic efficacy or toxic effects. The target of analysis is the underlying biochemical process or processes (i.e., metabolic process) thought to be involved in disease pathogenesis. Molecular flux rates within the one or more biochemical processes serve as biomarkers and are quantitated and compared with the molecular flux rates (i.e., biomarker) from control subjects (i.e., subjects not exposed to the compounds). Any change in the biomarker in the subject relative to the biomarker in the control subject provides information to evaluate therapeutic efficacy of an administered drug or a toxic effect and to develop the compound further if desired. In one aspect of the invention, stable isotope-labeled substrate molecules are administered to a subject and the label is incorporated into targeted molecules in a manner that reveals molecular flux rates through metabolic pathways of interest.

Single-use closed bioreactors with recirculating exhaust gas that can be operated in an uncontrolled environment are reported for the manufacturing of biological products using genetically modified biological cultures that produces carbon dioxide or that requires carbon dioxide in their metabolic process.

The invention discloses a method for biosynthesizing 2'-fucosyllactose by constructing recombinant colibacillus. The name of colibacillus genetic engineering bacteria is E.coli-XYY-1, a synthetic route of 2'-fucosyllactose is achieved, and meanwhile a construction method is disclosed. According to the method for biosynthesizing 2'-fucosyllactose by constructing recombinant colibacillus, existing technical problems are solved, and an efficient gene knockout scheme is obtained; after original strains are subjected to genetic reconstruction, 2'-fucosyllactose is produced, other characteristics of the strains are not changed, and fermentation production is not affected; plasmid adopted by the strains is mature colibacillus plasmid, and therefore growth and normal metabolism of bacteria are not affected in the metabolic process. The constructed recombinant colibacillus has a very large application prospect, and a new idea is provided for generating 2'-fucosyllactose through a biological method.

The invention is used as an additive to a culture medium to maintain and grow various cells including stem cells and support their manufactured and secreted products including cytokines, chemokines and growth factors in an environment which provides:a) metabolic enabling or enhancement of measurable parameters within the cellular populationb) availability and usage of nutrients to cellsc) distinct and positive effect on cellular dynamics (i.e. cellular proliferation and secretion of manufactured products by the cells)d) stabilized environment for maintaining conditions for growth and other cellular and metabolic processes including secretion of manufactured products including signaling factorse) enhanced biological cellular function of inherent cellular processesf) non-interference with normal cellular metabolics (i.e. signaling)g) increased protection from toxic effects to the cellh) additional benefits to the cellular population which in absence of the additive would be lacking.

The invention discloses a health-care food with a function of enhancing immunity and memory. The food comprises the following components in percentage by weight: 60-85% of pea albumen powder, 10-20% of whey protein powder, 1.5-10% of DHA (Docosahexaenoic Acid) algae oil, 1-10% of phosphatidylserine and 0.5-1% of phospholipid. According to the invention, pea albumen and whey protein are mixed by a certain proportion by means of a complementarity principle of animal and vegetable proteins, so that the structural proportion and number of amino acids better meet the demand of human body to the benefit of improving the immunity of human body and promoting synthesis of cerebral proteins. Meanwhile, pea albumen and phospholipid are matched in used to improve blood environment and prevent and treat lapse of memory caused by insufficient oxygen and blood supply to the brain. DHA and phosphatidylserine compounded have the correlating and prompting effect in the metabolic process of the brain of human body so as to enhance the memory and the like, so that the health-care food disclosed by the invention has the function of enhancing the immunity of human body and improving the memory of human body.

Products comprising an oxygen-generating additive for reducing the amount of skin irritation and inflamation and odor are disclosed. Specifically, products such as training pants and diapers are disclosed which contain a carbohydrate-hydrogen peroxide crystalline powder which, when wetted, produces a stream of oxygen which can be used by various bacteria on and near the wearer's skin during metabolism resulting in a significant decrease in the amount of volatile organic compounds produced by the bacteria during metabolism.

The present invention provides methods and compositions for modulating certain metabolic processes and for treating a variety of disorders associated with metabolic syndrome, including insulin related disorders, ischemia, oxidative stress, atherosclerosis, hypertension, obesity, abnormal lipid metabolism, and stroke by administering an effective dose of a chloroquine compound. The invention also provides methods and compositions relating to administering an effective dose of a chloroquine compound in combination with at least a second pharmaceutically active ingredient or compound including an antihyperglycemic diabetes treatment, an antihypertensive agent, an antithrombotic agent, and / or an inhibitor of cholesterol synthesis or absorption.

The invention discloses stewed shrimps for health care and nourishment having the effects of nourishing yin, strengthening yang and supplementing calcium. The stewed shrimps are prepared by the following steps of: selecting wildly fostered crayfishes and washing the crayfishes with clean water; cleaning the crayfishes; opening backs and removing bowels with a knife for later use; stirring and frying white sugar to be yellowish and pouring blend oil into a pan; putting ginger and seasonings for the health care and nourishment into the pan and stirring the materials slightly over small fire; adding flavorings into the pan; adjusting the fire to be big fire and putting the crayfishes into the pan; introducing the fire to burn the crayfishes to bright red with distilled spirit; adding broth to stew the crayfishes; adding gourmet powder or chicken essence; putting a little shallot and caraway into the pan; and taking the food out of the pan and placing the food in a bowl or a plate. The stewed shrimps for the health care and nourishment having the effects of nourishing yin, strengthening yang and supplementing the calcium overcomes the defect that the conventional crayfishes are usually stirred, cooked, steamed and fried directly so that the nutrition and the nourishment value of cooked crayfishes cannot be enhanced, can clear away harmful and toxic substances generated in the metabolic process of a human body and improve disease resistance of the organism, and has the effects of strengthening yang and blood, nourishing yin and conditioning the temperature, activating blood circulation to dissipate blood stasis, and removing pathogenic factors without any toxic or side effect.

This invention describes a novel, two-step method for administering PFC. The first step is designed to block the RES by administration of empty, small, liposomal vesicles (ESV) that are rapidly and preferentially engulfed by macrophages, thereby inhibiting their phagocytosis of subsequently infused PFC emulsions. The second step is the subsequent injection of PFC. ESV are devoid of materials that interfere with the macrophage's metabolic processes and do not impair their ability to clear the circulation of pathogenic organisms. Inhibition of the removal of PFC from the blood stream by the RES will achieve increased circulating PFC, enhanced binding and transport of oxygen throughout the blood stream and consequential reduction of undesirable consequences such as organomegaly and cytokine toxicity.

A system and method is provided for non-invasively measuring changes in a specimen suspected of containing one or more microbes, by monitoring changes in the dielectric constant of the specimen caused by metabolic processes of such microbes.

The invention discloses constructed recombinant escherichia coli and a method for biosynthesis of 3'-sialyllactose. The recombinant escherichia coli is named as E.coli-XYY which has a synthesis pathway of the 3'-sialyllactose. Meanwhile, the invention also discloses a constructing method. At the same time, the invention solves the problems of the prior art and provides an efficient gene knockout scheme; an original strain, after undergoing genetic engineering transformation, can produce the 3'-sialyllactose only, without changing other properties of the strain, so that influence on fermentation production is avoided; since mature escherichia coli plasmid is adopted by the strain, bacterium growth and normal metabolism do not affected in a metabolic process. The recombinant escherichia coli constructed by the invention has a good application prospect; therefore, a new thought is provided for the production of the 3'-sialyllactose by virtue of a biological method.

The invention discloses a feed additive, a preparation method and application of the feed additive. The preparation method of the feed additive comprises the steps of transferring activated lactobacillus strains to a sterilized MRS culture medium from glycerinum for culture to obtain a seed solution; carrying out primary and secondary enlarged cultivation on the seed solution in the MRS culture medium, inoculating into the sterilized solid culture medium for solid feed fermentation through an intermittent oxygen-introduction facultative anaerobic fermentation mode, and drying to obtain the feed additive. According to the preparation method of the feed additive, lactobacillus fermented soybean meal, bran, rice bran, straw, cottonseed cake, corncob powder, bagasse and other agricultural wastes are utilized for effectively reducing the antinutritional factor level in the raw materials, and the nutrition utilization rate is increased; the prepared feed additive is rich in antimicrobial peptide and other prebiotic substances secreted by lactobacillus through fermentation and metabolic processes, thereby having a prebiotic function and being healthy. The feed additive can be applied to an animal feed.

The invention provides plant antioxidation amino acid cleanser. The plant antioxidation amino acid cleanser is prepared from, by weight, 5-20% of glycerin, 1-5% of sodium lauroyl glutamate, 1-5% of sodium cocoyl glycinate, 0.1-0.5% of PEG-150 distearate, 0.5-2% of leuconostoc / radish root fermentation filer liquor, 0.1-5% of soapberry extracts, 0.1-5% of astaxanthin, 0.1-5% of ginkgo leaf extracts, 0.1-5% of Chinese yam extracts, 0.1-5% of panax notoginseng extracts, 0.1-5% of Chinese wolfberry extracts and the balance purified water. The plant antioxidation amino acid cleanser has the efficient cleaning function and is moderate in performance, meanwhile, the SOD activity can be improved, free radicals generated in the metabolic process are removed, and the antioxidation effect is achieved.

The invention discloses a preparation method of a fermented red ginseng product, which comprises the following steps of: processing a red ginseng and preparing a red ginseng juice; fermentation: placing the red ginseng juice into a fermentation pot and controlling the temperature at 30-35 DEG C, wherein the inoculation amount of lactobacillus acidophilus and lactobacillus plantarum is respectively 4-8 percent and 0.8-2.0 percent, the pH value is 3.0-4.0, and the fermentation time is 12-72 hours; and preparation of fermented red ginseng powder: drying the fermented red ginseng juice and then sieving to prepare the fermented red ginseng powder. The fermented red ginseng product is a novel health food developed by utilizing a biotransformation technology. The invention is characterized in that a fermentation method of finishing the metabolic process of bioactive components of the ginseng in vitro is adopted on the production process, so that the fermented red ginseng product has rapid absorption after administration, determined curative effect, no toxic or side effect and broad market prospect. Moreover, the processing process thereof is advanced, and the eating method of the product is simple and convenient.

The present invention is directed to methods of treating pest infestation by inhibiting metabolic processes of the pest such as for example, processes involved in invertebrate remodelling.

The present invention provides an organic silicon biological composition for seed soaking treatment or spraying treatment of crops. The organic silicon biological composition comprises a silatrane compound, an auxin-like compound and a compound water-soluble fertilizer, wherein the mass of the auxin-like compound is 5-12 times that of the silatrane compound and the mass of the compound water-soluble fertilizer is 500-1000 times that of the silatrane compound. The organic silicon biological composition provided by the invention has the beneficial effects that by adopting the silatrane compoundwith relatively high polarity as the active component, the growth and the development of plants can be accelerated by enhancing the synthesis of deoxyribonucleic acid (DNA), ribonucleic acid (RNA) andproteins; and by cooperating with the auxin-like compound and the compound water-soluble fertilizer, the composition can effectively participate in the metabolic process of the crops, the amount of heavy metals absorbed by the crops is reduced and the problems in the prior art that the application range of plant growth regulators is narrow and the growth and the development of the plants are easily affected are avoided. The invention also provides a using method of the organic silicon biological composition.

The invention relates to a method and a device for treating and recycling copper-containing acid wastewater of mines. The method comprises the following steps: mixing two wastewaters, namely copper pit water and raffinate, in a certain volume proportion, so as to form mixed acid wastewater; carrying out fractional precipitation on valuable heavy metals in the mixed acid wastewater by virtue of a combined medicament, so as to reduce neutralizing residue and recycle copper; recycling copper in the acid wastewater through the synergic effect generated in a metabolic process of chemical precipitates and sulfate reducing bacteria; and preparing a compound fertilizer from organic sludge generated by virtue of a biochemical method, and reusing the compound fertilizer in the rehabilitation and afforesting of the mines. According to the device for implementing the method, hydrogen sulfide circulating and absorbing equipment is additionally arranged, a reaction device is optimized, and combinedfillers of a bioreactor are optimized, so that the conversion efficiency of sulfate reducing bacteria to SO4<2-> is increased, and the repeated cyclic utilization of hydrogen sulfide is realized. A process adopted in the method is environment-friendly and has good universality to working conditions of the mines, so that the method has the advantages that the recycling rate of copper in the acid wastewater is more than or equal to 92%, the reduction amount of the neutralizing residue is more than or equal to 70%, the storage capacity pressure of tailings can be effectively reduced, and the like.

The invention provides a health care medicinal liquor for strengthening muscles and bones, and the health care medicinal liquor is prepared through repeated clinical test verification. The health care medicinal liquor accords with the principles of the traditional Chinese medicine. The health care medicinal liquor is prepared from the following raw traditional Chinese medicine materials: seeds ofChinese dodder, lotus stamen, herba epimedii, eucommia ulmoides, fructus psoraleae, cynomorium songaricum, cornu cervi degelatinatum, teasel roots, fructus ligustri lucidi, rhizoma cibotii, drynaria rhizome, centipedes, obscured homalomena rhizome, dog bones, chicken shells, pawpaw, fruit of Chinese wolfberry, poria cocos, schisandra chinensis, gentiana macrophylla, seeds of Chinese milk vetch, polygonum multiflorum, antler glue and raspberries. After being taken, the health care medicinal liquor has the functions of efficiently enhancing the bone mass, improving the bone quality, maintainingthe integrity degree of the microstructure of the bones, enhancing the activity of bone cells, and promoting the calcium deposition. The health care medicinal liquor can be used for relieving the pain of the waist, the back and the limbs, improving the microcirculation, increasing the bone density, adjusting the functions of the hypothalamus pituitary gland gonad, and improving the disorder status of the bone adjusting hormone and the cell factors in the metabolic process of the bones.

The invention discloses a preparation method of an organic-Se nutrient solution for crops. According to the method, bacillus subtilis is adopted to perform fermentation treatment on Se-enriched yeast, the Se-enriched yeast is hydrolyzed by the aid of protease produced in the metabolic process of the bacillus subtilis, a Se-enriched hydrolysate is obtained, and the organic-Se nutrient solution for the crops is prepared. The invention further discloses the organic-Se nutrient solution applied to the crops and produced with the preparation method of the organic-Se nutrient solution for the crops as well as an application of the organic-Se nutrient solution for the crops. With the adoption of the preparation method, all raw materials are sufficiently and reasonably utilized, complete and sufficient utilization of the raw materials is realized, no residues and residual liquids are produced, and environmental pollution is avoided; the content of organic-Se in the prepared organic-Se nutrient solution for the crops is high, the organic-Se exists in modes of Se substituted amino acid and short peptide, plant absorption is facilitated, and the content of the organic-Se in agricultural products can be remarkably increased.

The present invention provides methods and compositions for modulating certain metabolic processes and for treating a variety of disorders associated with metabolic syndrome, including insulin related disorders, ischemia, oxidative stress, atherosclerosis, hypertension, obesity, abnormal lipid metabolism, and stroke by administering an effective dose of a chloroquine compound. The invention also provides methods and compositions relating to administering an effective dose of a chloroquine compound in combination with at least a second pharmaceutically active ingredient or compound including an antihyperglycemic diabetes treatment, an antihypertensive agent, an antithrombotic agent, and / or an inhibitor of cholesterol synthesis or absorption.

The invention relates to a vitamin water aqua. The raw materials of the water aqua comprise liposome, vitamin A, vitamin D, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin H, folic acid and solubilizer. In the invention, liposoluble vitamins are coated in a microcapsule structure of the liposome and form a homogenized and stable water aqua with water-soluble vitamins. The water aqua has stable storage character in a long time, smaller grain diameter of components and a cytoid structure and can activate the self immunologic function after entering an animal body to keep the immune level of organisms in a higher state; and meanwhile, a plurality of vitamins can be effectively stored in the organism, so that the invention avoids the problems of the loss and the insufficient utilization rate of the vitamins in the metabolic process, remarkably improves the utilization rate and the conversion rate of feed, improves the antibody level of animal organisms and enhances the disease resistant capability.