36 results about "Metabolic Process" patented technology

The application discloses an Akkermansia muciniphila branched-chain amino acid metabolism gene nifJ and application thereof in colostrum IgA synthesis, and belongs to the technical field of genetic engineering. The application finds through in-vivo and in-vitro tests that the nifJ is a branched-chain amino acid metabolism gene of the Akkermansia muciniphila, regulates the metabolism process from branched-chain amino acids to branched-chain short-chain fatty acids, and plays a key role in the synthesis of maternal colostrum IgA. The research result of the application is expected to clarify the interaction relationship between the metabolic function of the Akkermansia muciniphila and a host, and reveal the action mechanism of the Akkermansia muciniphila in regulating the synthesis of maternal colostrum IgA, thereby providing a theoretical basis for improving the maternal colostrum IgA and further improving the maternal reproductive performance.

The application discloses an activated persulfate catalyst and a preparation method and application thereof, and relates to the technical field of catalysts.The catalyst is a bismuth-carbon intercalation catalyst in a chain structure, and the preparation method comprises the following steps: S1, taking a bismuth salt and an acyl benzene ligand as reaction substrates, and synthesizing Bi-MOFs through a hydrothermal reaction; and S2, calcining the Bi-MOFs at 500-700 DEG C under an inert gas atmosphere to obtain the bismuth-carbon intercalation catalyst.The bismuth-carbon intercalation catalyst activates persulfate through a non-radical direct electron transfer path, has higher activity and selectivity, can more effectively sterilize and disinfect specific structures and metabolic processes of target microorganisms, and improves the treatment efficiency; and can reduce the generation of by-products, effectively reduces the risk of the environment and the human body.

A process is disclosed for production of a polyhydroxyalkanoate that includes depolymerization of a post-consumer polyhydroxyalkanoate and utilization of the hydroxyalkanoate monomer thus produced as a carbon source for a microorganism capable of production of a polyhydroxyalkanoate. Methods can be utilized for true cyclic use of polyhydroxyalkanoates including polyhydroxybutyrates. Various aspects are described including simultaneous depolymerization and polymer production, utilization of purified depolymerase enzymes and / or microorganisms that express a depolymerase in conjunction with a microorganism that produces polymer, utilization of microorganisms that produce both a depolymerase and a new polymer, and utilization of genetically modified organisms to produce natural or modified depolymerase enzymes.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to application of a qi-tonifying and blood-maintaining preparation in preparation of a medicine for treating thalassemia. Researches show that the preparation for tonifying qi and maintaining blood is used for treating thalassemia and can effectively improve the anemia state and iron metabolism disorder of patients. Specifically, the medicine can significantly improve the hemoglobin level, increase the red blood cell count and the red blood cell average volume, and improve the serum iron content, the ferritin content and the reticulocyte proportion. The results show that the qi-tonifying and blood-maintaining preparation has the effect of promoting the bone marrow hematopoiesis function and can regulate and optimize the iron metabolism process. The invention provides an economical, effective and convenient treatment scheme for the thalassemia patient.

The invention provides a herbal medicine composition and a preparation for optimizing cell viability and resisting aging. The herbal medicine composition comprises the following components in percentage by weight: 5%-80% of a rhizoma polygonati extract, 5%-80% of a radix asparagi extract, 5%-80% of a pleurospermum tibetanum extract, 5%-80% of a mirabilis himalaica extract and 5%-80% of a tribulus terrestris extract. The herbal composition has a compound that protects cells from oxidative damage, enhances the cell repair mechanism, and improves the metabolic process with age decline; the composition is beneficial to optimization of cell activity and anti-aging, has wide benefits in anti-inflammatory, anti-oxidation, adaptability and other effects, is used for supporting overall activity, relieving pressure and enhancing immune function, has relatively low side effect risk, and supports anti-aging in a more comprehensive and more balanced manner.

The invention relates to the technical field of microbial fermentation, and discloses a pasture leavening agent for improving crude protein, reducing crude fiber and improving digestibility, which comprises three strains, namely aspergillus niger, candida utilis and lactobacillus pentosus. The invention relates to a self-induced peptide premix, which is composed of a covalent complex, a lignin-hemicellulose-L-arabinose, a phosphorylated urea-glycine chelate, a synthetic self-induced peptide premix and a carrier. According to the method, a specific initial carbon source is provided for aspergillus niger through LHLAC to start fiber degradation, synchronous slow release of a nitrogen source is achieved through PUGC, and protein anabolism of candida utilis is activated through sAIP. According to the scheme, a time sequence synergistic mechanism of fiber degradation, protein synthesis and acidification preservation is constructed, the problem of low efficiency caused by mismatching of all metabolic processes in the prior art is solved, and effective degradation of crude fibers of forage grass, great increase of crude protein content and remarkable improvement of final digestibility are realized.

The invention relates to clostridium perfringens in excrement as a molecular marker for grading and judging fatty liver diseases related to metabolic dysfunction and application of the clostridium perfringens, the clostridium perfringens contains alpha toxin CPA according to the types of main toxins generated by the clostridium perfringens, the coding gene of the alpha toxin CPA is plc gene, and the content of the alpha toxin CPA is determined by quantitatively detecting the plc gene. The molecular level detection of the clostridium perfringens in the excrement sample is realized; besides, the clostridium perfringens further contain nirA genes related to nitrogen metabolism, the nirA genes participate in the metabolic process related to microbial ammonia production, the functional state of the intestinal ammonia-producing microorganisms is reflected by detecting the expression level of the nirA genes, stable acquisition of the molecular characteristics of the intestinal microorganisms related to the MASLD is achieved, and the application prospect is broad. A molecular basis is provided for analyzing the correlation between the abnormal ammonia metabolism and the occurrence and development of the MASLD.

The invention discloses an intelligent management and control integrated system and method for brewing low-alcohol glutinous rice wine. The system comprises a sensing unit used for collecting multi-dimensional data such as flora, metabolic activity and physical and chemical parameters of fermentation mash in real time; the mechanism-data fusion control model is used for solving coupled kinetic equations of microbial growth, metabolic flow distribution and flavor substance synthesis on line, dynamically deducing a fermentation state and a quality trend and generating a regulation and control instruction set; and the execution module is used for synchronously executing flora regulation and control, physicochemical parameter regulation and control and metabolism guide instructions. Through cooperation of the modules, active prediction and guidance of a microbial metabolism network are realized, brewing control is fundamentally improved to a new normal form of intelligent regulation and control of an internal metabolism process from traditional environmental parameter tracking, and the alcoholic strength control precision, the flavor stability and the production batch consistency are remarkably improved.

The application discloses a small-molecule inhibitor STOCKIN-75990 for SLC6A14 and application of the small-molecule inhibitor in preparation of a drug for treating pancreatic cancer, the small-molecule inhibitor is a small-molecule compound for targeting amino acid transport protein SLC6A14, the small-molecule inhibitor regulates tumor cell amino acid uptake and metabolic process by targeting SLC6A14, so that growth and proliferation of pancreatic cancer cells are inhibited, and based on this, the SLC6A14 small-molecule inhibitor STOCKIN-75990 with high affinity and inhibitory activity is developed, and a new strategy is provided for targeted treatment of pancreatic cancer.

The invention discloses a synergetic embedding preparation process of bifidobacterium triple viable bacterium microcapsules, and relates to the technical field of microbial preparations, three viable bacteria are respectively loaded in three independent partitions by constructing a partition mutual-nutrition structure, so that small molecular nutrient substances and metabolites are allowed to penetrate through, the transboundary migration of strains can be prevented, and the synergistic embedding preparation process of the bifidobacterium triple viable bacterium microcapsules is realized. And the independence of the partition is ensured. Specific complementary nutrient substrates are added into all the partitions to form a mutual-nutrient metabolic chain, carrier protein is modified on the surface of the semipermeable membrane, the transfer efficiency of nutrient substances and metabolites is promoted, metabolic synergy of triple viable bacteria in an embedding system is achieved, and by selecting an inner-layer embedding wall material and an outer-layer embedding wall material of specific types and proportions, the effect of the triple viable bacteria in the embedding system is achieved. Synergistic matching of the degradation rate of the embedding material and the metabolism rate of the strain is achieved, and it is ensured that the strain can continuously obtain nutrition supply in the metabolism process.

The application discloses a detection method of traditional Chinese medicine-derived components in pine cone alcohol extract, and comprises the following steps: orally administering the pine cone alcohol extract to healthy animals, collecting plasma and fecal samples before and after administration, detecting and analyzing the samples by UPLC-MS / MS, and comparing the samples with the pine cone alcohol extract, so that eight blood components are found in the plasma sample after administration, and 18 components are found in the fecal sample after administration, which are all prototype components. The application comprehensively reflects the metabolism process and excretion path of the components in the pine cone in the body, and has important significance for guiding the clinical application and further research and development of the pine cone.

This invention belongs to the field of Antrodia camphorata cultivation technology, specifically relating to a method for cultivating Antrodia camphorata that can increase the triterpenoid content, comprising the following steps: S1. Inoculating the Antrodia camphorata strain into a liquid fermentation medium for initial liquid fermentation culture to obtain initial mycelium; S2. Transferring the initial mycelium to a Cinnamomum camphora culture medium for growth culture to obtain Antrodia camphorata; during the liquid fermentation culture process, periodic ultrasonic treatment is performed, the frequency of the ultrasonic treatment is 35kHz to 60kHz, with a treatment cycle of 2min-3min followed by an interval of 1min-2min, and the treatment is performed for 4h-6h per day. This invention combines liquid fermentation culture with solid culture, which improves production efficiency. Simultaneously, during the liquid fermentation culture stage, periodic ultrasonic treatment stimulates and accelerates the metabolic process of Antrodia camphorata, thereby increasing the content of triterpenoid compounds.

The application discloses a preparation method and application of a semiconductor mineral composite functional biomaterial for promoting reductive dechlorination, and belongs to the technical field of environmental biological remediation. The application solves the problems of slow metabolic rate and unstable degradation capacity of dechlorination respiratory bacteria in the existing biological remediation process. The semiconductor mineral is compounded with dechlorination respiratory bacteria such as pseudomonas aeruginosa, the conductive property of the semiconductor mineral is utilized, the electron transfer process in the microbial community and the growth and metabolism process of the dechlorination respiratory bacteria are promoted, and finally the microbial dechlorination of CPs is enhanced.

This invention discloses a fermentation medium for Monacolin K production by Monascus purpureus with added traditional Chinese medicine ingredients, specifically honeysuckle. Single-factor experiments were conducted to investigate the effects of honeysuckle on the growth and metabolism of Monascus purpureus. The results showed that adding 0.8 g of honeysuckle powder at 48 h of fermentation had the most significant promoting effect on Monacolin K synthesis, increasing Monacolin K production by up to 1.54 times, reaching 536.10 mg / L. Subsequent fermentation experiments indicated that honeysuckle provided abundant carbon and nitrogen sources and an acidic environment for the growth of Monascus purpureus, exhibiting a positive promoting effect on the entire metabolic process. The expression of nine genes related to Monacolin K synthesis also showed an upregulation trend. Simultaneously, the addition of honeysuckle significantly increased the content of total flavonoids and total polyphenols, and greatly enhanced the in vitro antioxidant activity of the fermentation products.

This invention discloses a dry heat treatment method for tomato seeds to enhance seed vigor and extend storage life, relating to the field of tomato seed storage technology. The method includes: selecting mature, plump, and undamaged commercial tomato seeds with consistent maturity and maintaining appropriate moisture content; placing the pre-treated seeds in a single layer on a tray and placing it in a dry heat treatment device, performing dry heat treatment sequentially according to a preset program; comparing the germination indicators of dry heat-treated and untreated seeds to determine the effect of the dry heat treatment; and sealing and storing tomato seeds with qualified germination rates. This invention, through precisely controlled stepwise heating and high-temperature constant-temperature sterilization, effectively activates the seed's internal repair enzyme system while inhibiting metabolic processes that lead to seed deterioration, avoiding physical or physiological damage to the seeds caused by high-temperature treatment, and effectively preventing seed coat cracking, radicle burn, or excessive dehydration.

This invention discloses the AgJHE1 gene and siRNA that interferes with the pesticide metabolism of cotton aphids, as well as their applications in the management of cotton aphid resistance, belonging to the field of biotechnology. The invention utilizes RNAi technology to interfere with the expression of the AgJHE1 gene, specifically designed siRNA based on the cotton aphid AgJHE1 gene. Results show that the siRNA enters the cotton aphid cells and triggers an RNAi cascade reaction, interfering with the expression of the target gene. Under pesticide stress, cotton aphids fed with siRNA showed a significantly increased sensitivity to the pesticide trifluralin. The siRNA provided by this invention can interfere with the detoxification and metabolic processes of cotton aphids to pesticides. This research contributes to understanding the metabolic resistance mechanism of cotton aphids and provides a new target for integrated pest management of resistant pests. The research results have significant theoretical and practical implications for the sustainable management of cotton aphids.

The invention relates to the technical field of metabolism simulation, in particular to a static simulation method for the metabolic process of pollutants coupling fish enteric microorganisms and digestive enzymes, which comprises the following steps: acquiring a fish enteric microorganism enrichment culture and an enteric enzyme extract, and analyzing the abundance of a fish enteric microorganism community and the variety of digestive enzymes; a static model composed of a gastric phase compartment, a midgut compartment and a hindgut compartment is constructed, and key incubation parameters such as culture medium volume, pH value, temperature, digestive enzyme activity and bile salt concentration of each compartment are determined. In the invention, the preparation and pre-stabilization method of the intestinal microbial liquid is particularly optimized, the authenticity of microbial metabolic activity is ensured, and the pairing relationship between pollution conversion and different digestion stages of fish is established by constructing a pollutant static metabolic conversion method of microbial community and digestive enzyme. The evaluation accuracy of the first-pass metabolic reaction of the pollutants is improved, and structural information of microorganisms, enzymes, the pollutants and substrates is integrated.

The present disclosure relates to a postbiotic composition that delivers a mixture or blend of postbiotic components in combination with micronutrients, said postbiotic components derived from beneficial bacteria from Lactiplantibacillus spp. or Lactobacillus spp. (L. plantarum, L. acidophilus, and L. rhamnosus). The postbiotic compositions of the disclosure may either be a live organism capable of proliferation in the subject, a non-living strain such as, but not limited to, a heat-killed proliferative strain, or a combination thereof. In addition to the postbiotic derived from Lactiplantibacillus or Lactobacillus beneficial bacterial strains, the compositions include micronutrients such as Vitamin D, vitamin B12, and herbal extracts in various combinations to ameliorate gut and metabolic health of a human subject.

This invention belongs to the field of biomedical engineering and discloses a method for extracting exovesicles from Gram-positive bacteria. The method involves culturing Gram-positive bacteria to the logarithmic growth phase to remove residual culture medium components, and then obtaining bacterial exovesicles derived from Gram-positive bacteria through multiple freeze-thaw cycles. These exovesicles contain active substances from the bacteria, such as proteins and small RNA molecules, which can participate in regulating physiological metabolic processes in vivo. Furthermore, they can also serve as drug delivery carriers, crossing membrane systems to target receptor cells via membrane signaling molecules, achieving targeted drug release.

The invention belongs to the technical field of microbial metabolic engineering modification. The invention provides an acetyl coenzyme A synthase mutant. The amino acid sequence of the acetyl coenzyme A synthase mutant is shown as SEQ ID No.1 in a sequence table. The acetyl coenzyme A synthase mutant provided by the invention is remarkably improved in the aspects of improving acetic acid metabolic activity and acetylation resistance, on one hand, the acetyl coenzyme A synthase mutant can be used for improving the efficiency that acetic acid is metabolized and converted by a strain, and on the other hand, accumulation of a byproduct acetic acid in a biological metabolism process can be reduced.

The application belongs to the technical field of oil and gas field fracturing, and particularly discloses a ternary microbial fracturing fluid system, a preparation method and application. The system first proposes a ternary synergistic composite system composed of a guar gum fracturing fluid base fluid, slick water and a source functional microbial agent screened in an oil field. The source functional microbial agent synchronously produces beneficial products such as a biological surfactant with excellent oil washing capacity during a metabolic process. The system completes reservoir hydraulic fracturing and fracture creation, and injects the source microorganism and its adaptive nutrition system into the deep reservoir during a pumping process. After gel breaking, the system realizes energy supplement of the formation, and the microorganism uses the gel breaking residual liquid as a nutrition source to start large-scale fermentation and oil displacement material production in situ in the fracture system, thereby realizing integration of fracturing, oil displacement and biological energy enhancement. The final oil displacement efficiency of the system can be increased to more than 50%, which is significantly improved compared with conventional fracturing fluid technology, and the system has the advantages of low reservoir damage, environmental friendliness, controllable cost, strong adaptability and the like.

The invention relates to a calcium-containing food supplement that differs from its counterparts in that it contains Lysine amino acid, which contributes to the absorption of calcium, and Arginine amino acids, which enable the utilisation of calcium in the metabolic process, which is generally developed for use in supporting bone and bone development metabolism, in order to support the prevention of Osteoporosis, Maleostasis, seen in women after menopause; in adult men, to contribute positively to the solution of problems in the absorption and utilisation of calcium that occur in bone metabolism with aging; To protect dental health and in dental implant treatment, to ensure the stability of the implant by surrounding the implant surface of the bone tissue; to ensure that children have stronger bones and, to the extent permitted by genetic factors, longer bones and therefore taller stature at the developmental age; Depending on various factors, to support the absorption and transport of calcium in skeletal problems, to support faster and better union of bone fractures.

This invention discloses a method for assessing lipid reesterification, belonging to the field of biochemistry. Based on radioisotope-labeled assisted liquid scintillation counting technology, this invention provides a method for evaluating the reesterification of functional lipids. For the reaction of fatty acids with coenzyme A to form acyl-CoA, coenzyme A is labeled with a radioisotope, and the reesterification rate of fatty acids is quantified by calculating the isotope recovery rate in the product. For the reaction of sterols with acyl-CoA to form sterol fatty acid esters, sterols and acyl-CoA are labeled with two different isotopes respectively. The reduction of acyl-CoA in the reactants and the formation of sterol fatty acid esters in the product are characterized by detecting the intensity of the two isotopes simultaneously carried in the product, thus quantifying the reesterification rate of sterols. This invention uses labeled coenzyme A as an evaluator for the metabolic process, precisely locating the lipid metabolic pathway to the esterification reaction involving coenzyme A, eliminating the influence of unknown metabolic pathways.

This invention, entitled "A Method, Radiolabeled Compound, and Application for Radiolabeling of Compounds Containing Carboxylic Acid, Phosphoric Acid, or Sulfate Groups," belongs to the field of radioisotope labeling preparation technology. The technical problem to be solved is to reduce operational difficulty and simplify the preparation process; and to better monitor the metabolic processes of macromolecules in vivo. The key technical solution is: a method for radiolabeling compounds containing carboxylic acid, phosphate, or sulfate groups, comprising the steps of reacting the compound containing carboxylic acid, phosphate, or sulfate groups, a solvent, a catalyst, and a radioactive reagent, followed by purification.

This invention discloses a *Lactobacillus johnsonii* strain LacY248 that regulates macrophage metabolism and its applications, belonging to the field of microbial technology, and particularly relating to a *Lactobacillus johnsonii* strain that regulates macrophage metabolism (…). Lactobacillus johnsonii i) LacY248, deposited on May 27, 2025, at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: M20251197. The *Lactobacillus johnsonii* LacY248 described in this invention has the function of alleviating enteritis caused by *Escherichia coli*. This strain can promote the metabolism of shikimic acid and cinnamic acid, and can inhibit macrophage polarization towards M1 by regulating macrophage metabolic activity, thereby inhibiting macrophage glycolysis and downregulating the expression levels of key glycolytic enzymes in macrophages.

The application belongs to the technical field of immunotherapy, and particularly relates to a method for improving the effect function of NK cells, wherein point mutation vectors PKM2-322K-R and PKM2-433K-R of a PKM2 gene are constructed, the glycolysis metabolic process is affected, the generation of lactic acid is reduced, and the lactic acidification modification level is inhibited. The application further provides a construction method of NK92MI cells of a PKM2 protein, and the steps include constructing a vector, extracting a plasmid, packaging and concentrating viruses, and transfecting target cells. The application targets lactic acidification modification, a protein modification, blocks lactic acidification modification, reverses the functional exhaustion of NK cells, makes PKM2 de-lactic acidification through point mutation, can partially reverse the function of NK cells, and restores the expression level of effect molecules of NK92MI cells.

The invention discloses a polypeptide and application thereof, and relates to the technical field of biology. The amino acid sequence of the polypeptide is shown as SEQ ID NO: 1. The polypeptide designed based on the precise binding site of the Gabp alpha and the Ppargamma comprises an aa164-189 amino acid sequence fragment of the Ppargamma, ARG168 and LYS184 sites can be specifically bound with ASP332 and GLN324 sites of the Gabp alpha respectively, a natural binding interface of the Gabp alpha and the Ppargamma can be competitively occupied, specific blocking of the interaction of the Gabp alpha and the Ppargamma is achieved, and the effect of inhibiting the Gabp alpha and the Ppargamma is achieved. Non-specific interference caused by interaction of other proteins is avoided, and a high-specificity tool is provided for targeted regulation of a brown adipose tissue related metabolic process.

The present application relates to the field of hydrological and hydrodynamic simulation, in particular to a hydrological and hydrodynamic process simulation method based on multiple GPUs, comprising the following steps: S1, obtaining basic data and pollutant data of a target area; S2, dividing the target area into a plurality of grid-shaped sub-regions, and assigning a GPU to each sub-region; S3, calculating the water flow data of each sub-region according to the basic data and the pollutant data, the water flow data being used to represent the speed and direction of the water flow; S4, calculating the pollutant concentration at each location at each time according to the water flow data; The present application takes into account the feedback mechanism between biological enrichment and biological degradation, can more accurately predict the pollutant concentration distribution in the water body at different time points, and can better simulate the metabolic process in the organism and the influence of environmental factors, so that the simulation result is closer to the actual situation.

The invention discloses a trehalose aggregation-induced emission fluorescent probe as well as a synthesis method and application thereof, and belongs to the technical field of fluorescent probes, the fluorescent probe with the special structure is synthesized for the first time, alanine and fluorescent molecules are directly coupled, trehalose participates in metabolism of a target object, and the fluorescence intensity of the target object is improved. Compared with biological orthogonal marking, direct marking is more convenient and is different from a metabolic marking approach in which amino acid participates, and the trehalose probe is expected to research the glycometabolism process of various bacteria and has important significance on research of life science.

The invention discloses poplar and phellinus igniarius polysaccharide SVP-50-1 as well as a preparation method and application thereof, and belongs to the field of biological medicines. The poplar phellinus igniarius polysaccharide SVP-50-1 is obtained by carrying out water extraction on poplar phellinus igniarius sporocarp and precipitating the poplar phellinus igniarius sporocarp with a 50% ethanol solution. The poplar phellinus igniarius polysaccharide SVP-50-1 provided by the invention can regulate the tumor metabolism process and inhibit the mitochondrial respiratory chain activity and the oxidative phosphorylation process of tumor cells, so that the energy source of the tumor cells is inhibited, and the anti-tumor effect is achieved. When the poplar and phellinus igniarius polysaccharide SVP-50-1 is combined with radiotherapy and chemotherapy drugs for use, a synergistic effect can be achieved. According to the combined treatment strategy, the curative effect of the existing tumor therapy can be remarkably improved, meanwhile, adverse reactions are reduced, and a new optimization scheme is provided for clinical tumor treatment.