67 results about "Metabolic Process" patented technology

The invention provides a Burkholderia paradensis (Paraburkholderia caledonica) and an application of the Burkholderia paradonica (Paraburkholderia caledonica). The strain provided by the invention can realize green and efficient recovery of various rare earth raw materials. According to the present invention, the bacterial strain can produce a variety of organic acids and secondary metabolites having complexing properties during the metabolism process, and the electron transfer process on the surface of the microbial cell membrane provides important effects in the leaching of different rare earth raw materials;

The invention discloses a screening and expression platform construction method for non-essential gene loci of corynebacterium glutamicum, and belongs to the technical field of biology. The center sites CGLRS02940, CGLRS03085, CGLRS08555, CGLRS09020 and CGLRS13560 of the corynebacterium glutamicum identified by the invention have the remarkable advantages that when gene knockout or knockin operation is carried out, the sites do not interfere with the normal physiological metabolism process of strains, and stable expression of exogenous genes can be guaranteed; meanwhile, the gene editing efficiency is relatively high, and an editing target with high universality and high reliability is provided for genetic engineering modification of corynebacterium glutamicum.

The application discloses an Akkermansia muciniphila branched-chain amino acid metabolism gene nifJ and application thereof in colostrum IgA synthesis, and belongs to the technical field of genetic engineering. The application finds through in-vivo and in-vitro tests that the nifJ is a branched-chain amino acid metabolism gene of the Akkermansia muciniphila, regulates the metabolism process from branched-chain amino acids to branched-chain short-chain fatty acids, and plays a key role in the synthesis of maternal colostrum IgA. The research result of the application is expected to clarify the interaction relationship between the metabolic function of the Akkermansia muciniphila and a host, and reveal the action mechanism of the Akkermansia muciniphila in regulating the synthesis of maternal colostrum IgA, thereby providing a theoretical basis for improving the maternal colostrum IgA and further improving the maternal reproductive performance.

The invention provides a screening method of a targeted drug for inhibiting accumulation of trimethylamine and / or trimethylamine-N-oxide. The screening method comprises the following steps: determining an action target on a metabolic pathway of trimethylamine and / or trimethylamine-N-oxide; if the target is protein, preliminarily screening candidate components based on the three-dimensional structure of the target; the candidate component is validated to confirm the targeted drug. According to the method, large-scale screening of targeted drugs aiming at targets can be achieved, the targeted drugs inhibit accumulation of trimethylamine and / or trimethylamine-N-oxide in the body by influencing the metabolic pathway of trimethylamine and / or trimethylamine-N-oxide, side effects are small, and the normal metabolic process of the human body is prevented from being influenced.

The invention discloses a broad-spectrum plant-source disease-resistant excitant for rice blast and a preparation method thereof, and belongs to the technical field of rice blast prevention and control, in the broad-spectrum plant-source disease-resistant excitant, potassium glutamate serves as amino acid salt, not only provides a nitrogen source for plants, but also participates in the metabolic process in the plants and enhances the stress resistance of the plants. S-allyl-L-cysteine is a sulfur-containing amino acid derivative, has antioxidant and disease-resistant activity, and can stimulate a defense mechanism of plants and resist infection of magnaporthe oryzae. 2-piperidinecarboxylic acid has the effect of inhibiting growth of pathogenic bacteria and can directly interfere with cell wall synthesis of magnaporthe oryzae, so that diseases are relieved. Chitosan as a biological polysaccharide can induce plants to generate resistant substances, enhance the strength of plant cell walls, form a physical barrier and prevent invasion of pathogenic bacteria. Salicylic acid is an important signal molecule in a plant body and can activate systematic resistance of the plant and improve the overall disease resistance.

The application discloses an activated persulfate catalyst and a preparation method and application thereof, and relates to the technical field of catalysts.The catalyst is a bismuth-carbon intercalation catalyst in a chain structure, and the preparation method comprises the following steps: S1, taking a bismuth salt and an acyl benzene ligand as reaction substrates, and synthesizing Bi-MOFs through a hydrothermal reaction; and S2, calcining the Bi-MOFs at 500-700 DEG C under an inert gas atmosphere to obtain the bismuth-carbon intercalation catalyst.The bismuth-carbon intercalation catalyst activates persulfate through a non-radical direct electron transfer path, has higher activity and selectivity, can more effectively sterilize and disinfect specific structures and metabolic processes of target microorganisms, and improves the treatment efficiency; and can reduce the generation of by-products, effectively reduces the risk of the environment and the human body.

The invention discloses a high-yield cell-free protein synthesis system based on a cell crude extract and application of the high-yield cell-free protein synthesis system. The system comprises an escherichia coli extract, a DNA template, a buffer solution, an amino acid mixture, a nucleoside triphosphate mixture, an ATP regeneration system and antibiotics, the antibiotics are selected from compounds having inhibitory activity on metabolic processes except ribosome functions in prokaryotic microorganisms, including but not limited to one or more combinations of interfering DNA replication, DNA transcription, cell wall synthesis, energy metabolism, lipid synthesis or other non-translational related metabolic pathways. According to the method disclosed by the invention, the escherichia coli crude extract is used as a bacterial chassis, and non-essential metabolic pathways in a CFPS system are selectively inhibited by introducing different types of antibiotics, so that the yield of protein synthesis is increased.

The invention belongs to the technical field of drug toxicity prediction, and particularly relates to a drug toxicity prediction method based on metabolism guidance. The invention aims to guide a prodrug or a non-toxic substance through a metabolism method and consider the toxicity of the prodrug or the non-toxic substance in the metabolic process (for example, the prodrug is converted into an effective component in the metabolic process), so as to predict the potential toxicity of the prodrug or the non-toxic substance. By means of the guidance of metabolism, it is desirable to accurately predict whether or not a prodrug will generate toxicity in the body. In the technical scheme provided by the invention, molecules are learned by using different characterization modes for the molecular map, so that different features under different levels and different segmentation methods can be captured. Through the guidance of the metabolic diagram, the reactants can obtain product-related attributes for explaining the toxicity change generated in the metabolic process of the reactants.

The application discloses a method for dynamically regulating the content of smell and taste substances in the secondary metabolism process of streptomyces, and relates to the technical field of molecular biology. The method for dynamically regulating the content of smell and taste substances in the secondary metabolism process of streptomyces disclosed by the application determines a first promoter and an sgRNA sequence capable of targeting a first target gene affecting the synthesis of a target compound according to the types of microorganisms and the target compound; a first recombinant plasmid is constructed by using a molecular cloning method; meanwhile, a second promoter for starting the synthesis of the target compound and a gene sequence capable of encoding a Cas protein losing cutting activity are determined, and the two are connected to obtain a regulation unit; the linearized enzyme cutting product after double enzyme cutting of the first recombinant plasmid is connected with the regulation unit to obtain a time-sequential dynamic inhibition recombinant plasmid, and after the recombinant plasmid is introduced into streptomyces, a streptomyces strain with reduced content of smell and taste (geosmin) substances in the secondary metabolism process is obtained.

The application discloses a non-natural sugar and a synthesis method and application thereof. At room temperature, hydroxyl groups of an amino sugar are fully trimethylsilyl-protected, amino groups on the sugar are selectively exposed, and after coupling and conversion of the amino groups at room temperature, a non-natural sugar with orthogonal groups and full trimethylsilyl protection is obtained. The non-natural sugar with orthogonal groups and full trimethylsilyl protection is removed from trimethylsilyl protection groups, and a non-natural sugar without protection groups is obtained. The application takes into account the advantages of existing non-natural sugars, ensures that the non-natural sugars can be efficiently utilized by cells, effectively avoids S side reactions with cysteine in proteins in the metabolic process of the non-natural sugars, and simultaneously realizes efficient metabolic labeling. In a cell test, the use concentration of 1,6-bisacylated non-natural sugar is one order of magnitude lower than that of non-natural sugar without protection groups.

The invention belongs to the technical field of analysis and detection, and particularly relates to a preparation method and application of a matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry plant sample, the preparation method of the plant sample created by the invention can separate leaf inner epidermis and mesophyll tissue, and the leaf inner epidermis and mesophyll tissue are utilized to research metabolites in leaves. After the inner epidermis and mesophyll of the leaf are separated, not only can organic acid, small molecular compounds and macromolecular substances in the leaf be detected, but also the change of the content of the organic acid can be revealed. According to the MALDI mass spectrometry technology, assimilation of NH4 < + > / NO3 <-> in leaf inner epidermis or mesophyll tissue can be monitored in an imaging mode, so that the metabolic process of nitrogen is visualized; in the nitrogen metabolism process, NH4 < + > is directly used for synthesizing amino acid, NO3 <-> is reduced into NO2 <->, and NO2 <-> is assimilated into amino acid and derivatives thereof. The preparation method provided by the invention has unique advantages, and is beneficial to deeply understanding the metabolic process and biological functions of plants.

The invention provides a covalent complex of human milk oligosaccharide and a fluorescent reagent and a preparation method of the covalent complex, and belongs to the field of organic chemistry. The application of the human milk oligosaccharide-cyanine dye Cy7 covalent complex provided by the invention in monitoring the metabolic distribution of the human milk oligosaccharide-cyanine dye Cy7 covalent complex in internal tissues and organs through fluorescence imaging has a good tracing effect, and can dynamically trace the metabolic process of human milk oligosaccharide in vivo at different time points; compared with a traditional liquid chromatography-mass spectrometry in-vitro drug metabolism detection method, the fluorescence tracing method has the advantage that the in-vivo metabolism condition of the human milk oligosaccharide can be accurately, intuitively and efficiently monitored.

The invention discloses a cinnamomum camphora MYB transcription factor gene, and belongs to the technical field of forest metabolism regulation. The nucleotide sequence of the gene is as shown in SEQ ID NO.5. The amino acid sequence of the transcription factor is as shown in SEQ ID No.7. The gene provided by the invention is one of key genes in a cinnamomum camphora metabolism process, and can regulate and control flavonoid metabolism, terpenoid metabolism, organic acid metabolism and the like of cinnamomum camphora. The gene provides an important theoretical basis for the industrial production in the fields of cinnamomum camphora breeding, medicines, spices and the like in the future, and has a wide application prospect and a great economic value.

The invention provides new application of an ATOX1 gene and ATOX1 protein, and belongs to the technical field of treatment of vitiligo diseases. The ATOX1 gene serving as a key gene of copper metabolism is disclosed for the first time, and melanin synthesis is promoted by activating the melanocyte copper metabolism process, so that the effect of resisting leucoderma is achieved. The ATOX1 gene and / or the ATOX1 protein can be used as a key biomarker in the vitiligo morbidity process, and a new target and a new molecular basis are provided for vitiligo research.

A process is disclosed for production of a polyhydroxyalkanoate that includes depolymerization of a post-consumer polyhydroxyalkanoate and utilization of the hydroxyalkanoate monomer thus produced as a carbon source for a microorganism capable of production of a polyhydroxyalkanoate. Methods can be utilized for true cyclic use of polyhydroxyalkanoates including polyhydroxybutyrates. Various aspects are described including simultaneous depolymerization and polymer production, utilization of purified depolymerase enzymes and / or microorganisms that express a depolymerase in conjunction with a microorganism that produces polymer, utilization of microorganisms that produce both a depolymerase and a new polymer, and utilization of genetically modified organisms to produce natural or modified depolymerase enzymes.

This invention relates to a propofol administration method based on deep learning-based pharmacokinetic-pharmacodynamic and reinforcement learning methods and its application. It uses a PKPD model to simulate the metabolic process of propofol in the human body, extracts time-dependent and kinetic continuous information through LSTM and Neural OED, and then establishes a nonlinear relationship between the effective concentration of propofol and the clinically observed anesthesia depth index (BIS) using a Kan network. This rapidly establishes a mapping between intraoperative monitoring information and anesthesia depth, allowing for timely adjustments to the strategy based on environmental changes. It is suitable for handling non-standardized and complex problems, enabling personalized adjustments to drug dosage and administration regimens, and improving therapeutic efficacy.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to application of a qi-tonifying and blood-maintaining preparation in preparation of a medicine for treating thalassemia. Researches show that the preparation for tonifying qi and maintaining blood is used for treating thalassemia and can effectively improve the anemia state and iron metabolism disorder of patients. Specifically, the medicine can significantly improve the hemoglobin level, increase the red blood cell count and the red blood cell average volume, and improve the serum iron content, the ferritin content and the reticulocyte proportion. The results show that the qi-tonifying and blood-maintaining preparation has the effect of promoting the bone marrow hematopoiesis function and can regulate and optimize the iron metabolism process. The invention provides an economical, effective and convenient treatment scheme for the thalassemia patient.

The invention provides a herbal medicine composition and a preparation for optimizing cell viability and resisting aging. The herbal medicine composition comprises the following components in percentage by weight: 5%-80% of a rhizoma polygonati extract, 5%-80% of a radix asparagi extract, 5%-80% of a pleurospermum tibetanum extract, 5%-80% of a mirabilis himalaica extract and 5%-80% of a tribulus terrestris extract. The herbal composition has a compound that protects cells from oxidative damage, enhances the cell repair mechanism, and improves the metabolic process with age decline; the composition is beneficial to optimization of cell activity and anti-aging, has wide benefits in anti-inflammatory, anti-oxidation, adaptability and other effects, is used for supporting overall activity, relieving pressure and enhancing immune function, has relatively low side effect risk, and supports anti-aging in a more comprehensive and more balanced manner.

The invention discloses an active substance for repairing a cat sensitization type barrier and a preparation method and application thereof, and relates to the technical field of animal health care products. The cat feed additive comprises one or more of heartleaf houttuynia herb extract, green tea polyphenol, ginkgo leaf extract, glutamine, beta-glucan, linoleic acid, vitamin B6, vitamin B2 and the like, and the components jointly act on oxidative stress, inflammatory response and metabolic process of intestinal tracts and skin of cats. By measuring indexes such as inflammatory factors, oxidative stress and tight junction protein and combining in-vitro metabonomics and transcriptomics analysis, the pharmaceutical composition has remarkable effects in the aspects of oxidation resistance, inflammation resistance, mucosal barrier repair and the like. In addition, the invention also provides application of the active substance in preparation of food for repairing cat barrier dysfunction. Compared with the prior art, an effective health-care strategy can be provided, and cat barrier dysfunction can be remarkably repaired.

The invention relates to the technical field of microbial fermentation, and discloses a pasture leavening agent for improving crude protein, reducing crude fiber and improving digestibility, which comprises three strains, namely aspergillus niger, candida utilis and lactobacillus pentosus. The invention relates to a self-induced peptide premix, which is composed of a covalent complex, a lignin-hemicellulose-L-arabinose, a phosphorylated urea-glycine chelate, a synthetic self-induced peptide premix and a carrier. According to the method, a specific initial carbon source is provided for aspergillus niger through LHLAC to start fiber degradation, synchronous slow release of a nitrogen source is achieved through PUGC, and protein anabolism of candida utilis is activated through sAIP. According to the scheme, a time sequence synergistic mechanism of fiber degradation, protein synthesis and acidification preservation is constructed, the problem of low efficiency caused by mismatching of all metabolic processes in the prior art is solved, and effective degradation of crude fibers of forage grass, great increase of crude protein content and remarkable improvement of final digestibility are realized.

The invention relates to clostridium perfringens in excrement as a molecular marker for grading and judging fatty liver diseases related to metabolic dysfunction and application of the clostridium perfringens, the clostridium perfringens contains alpha toxin CPA according to the types of main toxins generated by the clostridium perfringens, the coding gene of the alpha toxin CPA is plc gene, and the content of the alpha toxin CPA is determined by quantitatively detecting the plc gene. The molecular level detection of the clostridium perfringens in the excrement sample is realized; besides, the clostridium perfringens further contain nirA genes related to nitrogen metabolism, the nirA genes participate in the metabolic process related to microbial ammonia production, the functional state of the intestinal ammonia-producing microorganisms is reflected by detecting the expression level of the nirA genes, stable acquisition of the molecular characteristics of the intestinal microorganisms related to the MASLD is achieved, and the application prospect is broad. A molecular basis is provided for analyzing the correlation between the abnormal ammonia metabolism and the occurrence and development of the MASLD.

The invention discloses an intelligent management and control integrated system and method for brewing low-alcohol glutinous rice wine. The system comprises a sensing unit used for collecting multi-dimensional data such as flora, metabolic activity and physical and chemical parameters of fermentation mash in real time; the mechanism-data fusion control model is used for solving coupled kinetic equations of microbial growth, metabolic flow distribution and flavor substance synthesis on line, dynamically deducing a fermentation state and a quality trend and generating a regulation and control instruction set; and the execution module is used for synchronously executing flora regulation and control, physicochemical parameter regulation and control and metabolism guide instructions. Through cooperation of the modules, active prediction and guidance of a microbial metabolism network are realized, brewing control is fundamentally improved to a new normal form of intelligent regulation and control of an internal metabolism process from traditional environmental parameter tracking, and the alcoholic strength control precision, the flavor stability and the production batch consistency are remarkably improved.

The invention discloses an active substance for repairing canine intestinal mucosal barrier and a preparation method and application thereof, and relates to the technical field of animal health care products. The composition comprises one or more of centella asiatica extract, aloe polysaccharide, ginkgo biloba extract, glutamine, beta-glucan, vitamin B6, vitamin B2 and the like, and the components jointly act on oxidative stress, inflammatory response and metabolic process of canine intestinal tracts. By measuring indexes such as inflammatory factors, oxidative stress and tight junction protein and combining in-vitro metabonomics and transcriptomics analysis, the pharmaceutical composition has remarkable effects in the aspects of oxidation resistance, inflammation resistance, mucosal barrier repair and the like. In addition, the invention also provides application of the active substance in preparation of food for repairing dog intestinal mucosa. Compared with the prior art, an effective health-care strategy can be provided, and the intestinal mucosal barrier of the dog can be remarkably repaired.

The invention provides phosphorylation modified and dephosphorylation modified UDP-glycosyl transferase and application thereof, and belongs to the technical field of biology and quantitative phosphorylation proteomics. According to the invention, through early-stage phosphorylation proteomics data analysis and CsUGT84A22 protein sequence analysis in tea trees, phosphorylation modification sites exist in the CsUGT84A22 protein sequence. Protein phosphorylation modification is mostly reported to participate in regulation and control of plant growth and physiological metabolism processes, phosphorylation modified UDP-glycosyltransferase and dephosphorylation modified UDP-glycosyltransferase are provided, and the result shows that the invention discloses the influence of phosphorylation modification on synthesis of tea tree catechin compounds, and the application of the phosphorylation modified UDP-glycosyltransferase in regulation and control of plant growth and physiological metabolism processes is provided. The synthesis route of gallocatechin can be regulated and controlled, and the content of EGCG (epigallocatechin gallate) in tea trees can be changed, so that the bitterness of plants such as the tea trees and the like can be regulated and controlled. And a theoretical foundation is laid for analyzing a regulatory pathway of related phosphorylated protein in a tea tree flavonoid metabolic pathway.

The application discloses a small-molecule inhibitor STOCKIN-75990 for SLC6A14 and application of the small-molecule inhibitor in preparation of a drug for treating pancreatic cancer, the small-molecule inhibitor is a small-molecule compound for targeting amino acid transport protein SLC6A14, the small-molecule inhibitor regulates tumor cell amino acid uptake and metabolic process by targeting SLC6A14, so that growth and proliferation of pancreatic cancer cells are inhibited, and based on this, the SLC6A14 small-molecule inhibitor STOCKIN-75990 with high affinity and inhibitory activity is developed, and a new strategy is provided for targeted treatment of pancreatic cancer.

The invention discloses a synergetic embedding preparation process of bifidobacterium triple viable bacterium microcapsules, and relates to the technical field of microbial preparations, three viable bacteria are respectively loaded in three independent partitions by constructing a partition mutual-nutrition structure, so that small molecular nutrient substances and metabolites are allowed to penetrate through, the transboundary migration of strains can be prevented, and the synergistic embedding preparation process of the bifidobacterium triple viable bacterium microcapsules is realized. And the independence of the partition is ensured. Specific complementary nutrient substrates are added into all the partitions to form a mutual-nutrient metabolic chain, carrier protein is modified on the surface of the semipermeable membrane, the transfer efficiency of nutrient substances and metabolites is promoted, metabolic synergy of triple viable bacteria in an embedding system is achieved, and by selecting an inner-layer embedding wall material and an outer-layer embedding wall material of specific types and proportions, the effect of the triple viable bacteria in the embedding system is achieved. Synergistic matching of the degradation rate of the embedding material and the metabolism rate of the strain is achieved, and it is ensured that the strain can continuously obtain nutrition supply in the metabolism process.

The application provides Paraburkholderia caledonica and application thereof. The strain of the application can realize green and efficient recovery of various rare earth raw materials. In the application, the strain produces various organic acids and secondary metabolites with complexing properties in the metabolic process, and the electron transfer process on the surface of the microbial cell membrane plays an important role in leaching different rare earth raw materials.

The application discloses a detection method of traditional Chinese medicine-derived components in pine cone alcohol extract, and comprises the following steps: orally administering the pine cone alcohol extract to healthy animals, collecting plasma and fecal samples before and after administration, detecting and analyzing the samples by UPLC-MS / MS, and comparing the samples with the pine cone alcohol extract, so that eight blood components are found in the plasma sample after administration, and 18 components are found in the fecal sample after administration, which are all prototype components. The application comprehensively reflects the metabolism process and excretion path of the components in the pine cone in the body, and has important significance for guiding the clinical application and further research and development of the pine cone.

This invention belongs to the field of Antrodia camphorata cultivation technology, specifically relating to a method for cultivating Antrodia camphorata that can increase the triterpenoid content, comprising the following steps: S1. Inoculating the Antrodia camphorata strain into a liquid fermentation medium for initial liquid fermentation culture to obtain initial mycelium; S2. Transferring the initial mycelium to a Cinnamomum camphora culture medium for growth culture to obtain Antrodia camphorata; during the liquid fermentation culture process, periodic ultrasonic treatment is performed, the frequency of the ultrasonic treatment is 35kHz to 60kHz, with a treatment cycle of 2min-3min followed by an interval of 1min-2min, and the treatment is performed for 4h-6h per day. This invention combines liquid fermentation culture with solid culture, which improves production efficiency. Simultaneously, during the liquid fermentation culture stage, periodic ultrasonic treatment stimulates and accelerates the metabolic process of Antrodia camphorata, thereby increasing the content of triterpenoid compounds.

The application discloses a preparation method and application of a semiconductor mineral composite functional biomaterial for promoting reductive dechlorination, and belongs to the technical field of environmental biological remediation. The application solves the problems of slow metabolic rate and unstable degradation capacity of dechlorination respiratory bacteria in the existing biological remediation process. The semiconductor mineral is compounded with dechlorination respiratory bacteria such as pseudomonas aeruginosa, the conductive property of the semiconductor mineral is utilized, the electron transfer process in the microbial community and the growth and metabolism process of the dechlorination respiratory bacteria are promoted, and finally the microbial dechlorination of CPs is enhanced.