169 results about "Metabolic Process" patented technology

The invention relates to a method for identifying a disease microorganism-metabolite combined marker based on a host-flora co-metabolism model. The method comprises the following steps: processing microbiome data; setting diet constraint conditions; determining classification composition of intestinal microorganisms of each individual, mapping abundance of each microorganism to a human microorganism genome scale metabolism model, and constructing an individualized flora metabolism network model; carrying out metabolic flow simulation on the constructed flora metabolism network model; integrating the constructed flora metabolism network model with the host metabolism network model, and predicting the metabolic flow and metabolite change of the whole system; and establishing a disease classifier based on a random forest machine learning algorithm to identify the microorganism-metabolite combined marker. According to the method, the complex metabolic process between the human body and the intestinal flora is restored in a metabolic network modeling and metabolic flow simulation mode, the method has the non-invasive characteristic, the time and economic cost is remarkably reduced, and meanwhile, the method has a great advantage in accuracy.

The invention relates to the technical field of biological medicine, in particular to a dynamic fermentation tank control system for producing a diabetes intestinal microecological regulating preparation. The system comprises a fermentation tank sensing feedback control network establishment module, a fermentation tank growth earlier-stage nutrition supply control module, a fermentation tank growth later-stage adjustment control module and a fermentation tank metabolism process environmental condition control module, and can generate an embedded sensing feedback control network by installing a sensor and being connected with a central feedback control system. The sugar source supply quantity, the nitrogen source supply quantity, the temperature, the pH value, the dissolved oxygen concentration and the oxygen and carbon dioxide gas proportional flow corresponding to the fermentation tank are synchronously monitored in real time, so that supply cooperative dynamic control and fermentation growth cooperative dynamic control are carried out, and meanwhile, metabolism environment condition cooperative dynamic control is carried out; therefore, the cooperative control work of the microbial growth environment conditions is executed. By optimizing environment control in the fermentation process, efficient production of the intestinal microecology regulating preparation can be achieved.

The present disclosure belongs to the technical fields of computational medicine and clinical oncology, and specifically relates to a molecular subtyping method for gastric cancer (GC) based on metabolic genes, a construction method for a prognostic prediction model and applications. In the present disclosure, metabolic process alterations are overall assessed, and new metabolic transcription patterns based on 456 metabolic gene-related signatures are identified. Three different metabolic patterns are validated by unsupervised consensus clustering analysis and independent datasets, and molecular and clinical pathological features of these three metabolite clusters have different metabolic gene expressions, pathway enrichments, gene variations, and survival outcomes. In addition, in the present disclosure, a scoring scheme is constructed to assess metabolic patterns of individual tumors, revealing the relationships between the metabolic patterns with prognosis, cuproptosis and immune regulation, and having a good value for practical application.

The invention belongs to the technical field of biological medicines, and particularly relates to a pharmaceutical composition for preventing / treating Alzheimer's disease. According to the pharmaceutical composition based on the NPAFP protein provided by the invention, the synergistic effect of natural and artificially synthesized compounds is utilized, so that the pharmaceutical composition can play a role in a plurality of pathological levels, and the AD symptom is remarkably improved. The composition has the remarkable effects of resisting oxidation, resisting inflammation, promoting A beta removal, improving mitochondrial quality, reducing A beta 42 secretion and pathological states caused by Tau PFF and the like, and can protect neurons by adjusting the metabolic process. By screening and optimizing the compound with synergistic effect, the action mechanism of the compound is further defined, a novel safe and efficient potential solution is provided for AD treatment, and the compound has wide clinical application prospect. The medicine is high in safety, strong in specificity, simple and convenient in preparation method and suitable for mass production.

The invention discloses a satiety sugar-controlling fat-reducing composition and a preparation method and application thereof, the satiety sugar-controlling fat-reducing composition comprises hydrogel particles, a white kidney bean extract, a mulberry leaf extract, alpha-lipoic acid, L-carnitine, a lotus leaf extract, medium chain triglyceride, polyethylene glycol and sorbitol, and the composition is a tablet. The components are matched with one another to effectively adjust the leptin level, improve appetite, increase energy consumption, adjust the fat metabolism process, reduce fat accumulation, improve fat consumption, inhibit the activity of alpha-amylase, reduce the conversion of carbohydrates into fat, block heat and fat intake, promote gastrointestinal motility and enhance the immunity of the human body. The effects of remarkably reducing the fat content and the body weight are achieved.

The invention belongs to the field of leather making cleaning, and particularly relates to a leather bio-based degreasing agent and a preparation method thereof. The bio-based degreasing agent for leather comprises the following raw materials in percentage by weight: 18-22% of lipase, 38-42% of a bio-based surfactant, 0.1-0.2% of an enzyme activator, 1-3% of an enzyme stabilizer, 1-3% of a pH stabilizer and the balance of water. According to the leather bio-based degreasing agent disclosed by the invention, lipase and a bio-based surfactant (a metabolite with certain surface activity is secreted in a metabolic process when microorganisms are cultured under a certain condition) are organically combined, so that the lipase and the bio-based surfactant play a synergistic effect; and according to the detection result, the degreasing rate of the products obtained in the embodiments 1-3 is far higher than that of similar products sold in the market. In addition, the bio-based surfactant, lipase and other bio-based materials have great advantages in the aspects of carbon emission reduction, reproducibility and the like compared with a traditional petroleum-based surfactant, and popularization is more facilitated.

The invention provides a Burkholderia paradensis (Paraburkholderia caledonica) and an application of the Burkholderia paradonica (Paraburkholderia caledonica). The strain provided by the invention can realize green and efficient recovery of various rare earth raw materials. According to the present invention, the bacterial strain can produce a variety of organic acids and secondary metabolites having complexing properties during the metabolism process, and the electron transfer process on the surface of the microbial cell membrane provides important effects in the leaching of different rare earth raw materials;

The invention discloses a method for producing MK-7 through whole-cell regulation and control by using different time sequence type promoters, recombinant escherichia coli and application. According to the method, the time sequence type promoters are used for regulating and controlling expression of different key genes related to MK-7 synthesis. According to the invention, the time sequence promoter is used for whole-cell regulation and control to produce the vitamin MK-7 for the first time, dynamic regulation of the MK-7 is realized, and the production efficiency of the MK-7 can be remarkably improved by accurately regulating and controlling expression of key genes in a synthesis route of the MK-7 and optimizing an intracellular metabolic process. The promoter disclosed by the invention can regulate cell metabolism and enhance synthesis of MK-7 in different growth stages or under different environmental conditions by controlling the time and strength of gene expression. The MK-7 is produced by adopting the constructed recombinant escherichia coli obtained based on whole-cell regulation of the sequential promoter, and the yield of 14 g / L can be finally achieved in a 5L fermentation tank.

The invention relates to the technical field of microbial metabolism, in particular to a biological reagent production optimization method and system based on microbial metabolism characteristics. The method comprises the following steps: acquiring a biological reagent to produce a microbial strain, and determining the component proportion of a microbial culture medium and optimal microbial culture conditions; carrying out microorganism culture metabolism recording and metabolic flow path analysis on the biological reagent production microorganism strain, and simultaneously carrying out metabolic characteristic analysis to obtain metabolic quantitative characteristics of the biological reagent production microorganism; carrying out metabolic bottleneck node identification analysis on the metabolic flow path of the biological reagent production microorganisms to obtain metabolic bottleneck nodes of the biological reagent production microorganisms; and carrying out microbial production optimization on the corresponding biological reagent production microbial culture metabolism process, and carrying out production post-treatment and purification separation to obtain a biological reagent production optimization product. The production efficiency of the biological reagent can be remarkably improved, so that the stability and the consistency of the biological reagent product are ensured.

A method of detecting vitamin D in blood using laser desorption / ionization mass spectrometry (LDI-MS) and an apparatus therefor according to the present invention are not complicated in a measurement, do not require a number of measurement steps, and allow for easy measurement and collection of results in real time with a quick analysis. In addition, precise analysis may be performed even at a lower concentration of a sample, such that sensitivity and precision are excellent, various subtypes of vitamin D may be simultaneously detected, a throughput is high, and structural analysis and quantitative analysis of vitamin D that has undergone a metabolic process in blood may be accurately performed without a matrix interference.

The invention relates to an ecological risk dynamic simulation evaluation and prediction early warning method based on a material metabolism process, and the method comprises the following steps: 1, ecological risk impact recognition: precisely analyzing the urban metabolism process through dynamic material flow analysis, and constructing an urban metabolism network model; step 2, ecological risk dynamic simulation evaluation: the risk impact of each metabolic subject is incorporated into an input-output model, and an urban ecological risk diffusion model is constructed; step 3, ecological risk prediction and early warning: constructing a prediction model of ecological risk impact indexes and influence factors based on an expandable random environmental influence evaluation model; the method has the advantages that key metabolic risk points are mined through risk impact recognition and quantification of all metabolic subjects, influence linkage conduction process simulation, risk index calculation and metabolic risk prediction and early warning, and stable operation of an urban agglomeration system is ensured; starting from the whole metabolic process of urban source supply and the like, the mechanism of metabolic risk is systematically explored.

The invention belongs to the field of escherichia coli metabolic processes, and particularly relates to a method for producing D-psicose from escherichia coli through glucose fermentation. Escherichia coli is used as a chassis host bacterium, a pgi gene, an SUMO-alsE fusion gene and an a6PP gene are subjected to overexpression at the same time, then a pfkA gene, a pfkB gene and a rpiA gene are knocked out in sequence, and a recombinant bacterium capable of producing D-psicose through glucose fermentation is obtained; the recombinant bacteria are used for fermentation, and glucose is converted to generate D-psicose.

The invention discloses a screening and expression platform construction method for non-essential gene loci of corynebacterium glutamicum, and belongs to the technical field of biology. The center sites CGLRS02940, CGLRS03085, CGLRS08555, CGLRS09020 and CGLRS13560 of the corynebacterium glutamicum identified by the invention have the remarkable advantages that when gene knockout or knockin operation is carried out, the sites do not interfere with the normal physiological metabolism process of strains, and stable expression of exogenous genes can be guaranteed; meanwhile, the gene editing efficiency is relatively high, and an editing target with high universality and high reliability is provided for genetic engineering modification of corynebacterium glutamicum.

The present invention relates to the field of biomedicine technology, and provides the use of an upregulator of ACOT2 in promoting liver regeneration and liver function recovery after partial hepatectomy. Specifically, it provides the use of an ACOT2 expression promoter in the preparation of a drug for promoting liver regeneration and liver function recovery after partial hepatectomy, as well as a pharmaceutical composition comprising the ACOT2 expression promoter. The present invention has developed new discoveries about the function of the ACOT2 gene. The increased expression of ACOT2 after hepatectomy has a certain protective effect on liver regeneration, or can serve as a potential marker for warning the degree of postoperative liver function damage and liver regeneration capacity, and a therapeutic target for promoting postoperative liver regeneration. By using the intervention method of the present invention, the decomposition and consumption of lipid droplets in the liver lipid metabolism process are targeted, thereby promoting the recovery of liver function and liver-to-body ratio.

The present invention provides a Bacillus tequilensis ZW-35 and its application, which relates to the technical field of microbial control. The Bacillus tequilensis ZW-35 is preserved in the China General Microbiological Culture Collection Center, and its taxonomic name is Bacillus tequilensis Bacillus tequilensis , and the preservation number is CGMCC No. 32878. The volatile substances in the metabolic process of Bacillus tequilensis ZW-35 can effectively inhibit the growth of Colletotrichum; and the volatile substances have high diffusion ability in air and soil pores. In the prevention and control of fruit diseases, the volatile substances can avoid direct contact with the fruits and will not leave residues on the fruit surface, thus avoiding the potential risks brought by direct contact.

The application discloses an Akkermansia muciniphila branched-chain amino acid metabolism gene nifJ and application thereof in colostrum IgA synthesis, and belongs to the technical field of genetic engineering. The application finds through in-vivo and in-vitro tests that the nifJ is a branched-chain amino acid metabolism gene of the Akkermansia muciniphila, regulates the metabolism process from branched-chain amino acids to branched-chain short-chain fatty acids, and plays a key role in the synthesis of maternal colostrum IgA. The research result of the application is expected to clarify the interaction relationship between the metabolic function of the Akkermansia muciniphila and a host, and reveal the action mechanism of the Akkermansia muciniphila in regulating the synthesis of maternal colostrum IgA, thereby providing a theoretical basis for improving the maternal colostrum IgA and further improving the maternal reproductive performance.

The invention provides a screening method of a targeted drug for inhibiting accumulation of trimethylamine and / or trimethylamine-N-oxide. The screening method comprises the following steps: determining an action target on a metabolic pathway of trimethylamine and / or trimethylamine-N-oxide; if the target is protein, preliminarily screening candidate components based on the three-dimensional structure of the target; the candidate component is validated to confirm the targeted drug. According to the method, large-scale screening of targeted drugs aiming at targets can be achieved, the targeted drugs inhibit accumulation of trimethylamine and / or trimethylamine-N-oxide in the body by influencing the metabolic pathway of trimethylamine and / or trimethylamine-N-oxide, side effects are small, and the normal metabolic process of the human body is prevented from being influenced.

The invention relates to the use of at least one inhibitory structural analog or inhibitory functional analog of a coenzyme (such as thiamine for example) of an enzyme group, the enzyme members of which catalyze anabolic and / or catabolic and / or energy-releasing metabolic reactions that are of essential significance for the functionality of the overall metabolism of cells, in particular mammalian cells. The invention is used to treat patients in order to produce a general successive (in particular also continuous) slowing down of the metabolic processes of endogenous cells and exogenous cells in the body of the patient and thus achieve a slowing down of disease-causing processes in particular.

The invention discloses a method for calculating unstable flow change of biological network metabolism. The method comprises the following steps: loading a metabolism calculation model; inputting the total system reaction time T and the time step delta t into the metabolism calculation model, obtaining the mass isotope distribution change state of the intermediate metabolite, and obtaining the prediction state of the metabolic reaction flow; the change state of the mass isotope distribution of the intermediate metabolite is composed of a change value of the mass isotope distribution of the intermediate metabolite along with time and a metabolism time point t; the prediction state is composed of a flow value of a metabolic reaction at a metabolic time point t and the metabolic time point t; the metabolism calculation model comprises an intermediate metabolite concentration change calculation unit, an intermediate metabolite mass isotope distribution change calculation unit, a sample processing unit and a neural network construction unit. According to the technical scheme, calculation and prediction of the unstable flow change of biological network metabolism in the metabolic process can be achieved, and the prediction result is more accurate.

The invention discloses a broad-spectrum plant-source disease-resistant excitant for rice blast and a preparation method thereof, and belongs to the technical field of rice blast prevention and control, in the broad-spectrum plant-source disease-resistant excitant, potassium glutamate serves as amino acid salt, not only provides a nitrogen source for plants, but also participates in the metabolic process in the plants and enhances the stress resistance of the plants. S-allyl-L-cysteine is a sulfur-containing amino acid derivative, has antioxidant and disease-resistant activity, and can stimulate a defense mechanism of plants and resist infection of magnaporthe oryzae. 2-piperidinecarboxylic acid has the effect of inhibiting growth of pathogenic bacteria and can directly interfere with cell wall synthesis of magnaporthe oryzae, so that diseases are relieved. Chitosan as a biological polysaccharide can induce plants to generate resistant substances, enhance the strength of plant cell walls, form a physical barrier and prevent invasion of pathogenic bacteria. Salicylic acid is an important signal molecule in a plant body and can activate systematic resistance of the plant and improve the overall disease resistance.

The utility model relates to the technical field of medical detection equipment, in particular to an in-vitro micro-tissue drug metabolism detection device. Comprising an incubation chamber, a printing terminal and an auxiliary assembly, a temperature control assembly is arranged at the top of the incubation chamber, the auxiliary assembly comprises a first conveyor, a second conveyor, a sorting robot, a sample pool and a detection component, the incubation chamber is used for incubating samples, the incubated samples are placed on the first conveyor to be conveyed, and after being detected by the detection component, the samples are sent to the printing terminal. After detection, samples can be rapidly placed on the second conveyor to be conveyed, finally, the samples are placed into the sample pools at different positions through the sorting robot according to feedback information of the control system, the printing terminal can print and output detection data, then the in-vivo microenvironment can be simulated, the in-vivo metabolic process of drugs can be accurately simulated, and the in-vivo microenvironment can be simulated. Moreover, preliminary detection and rapid transfer of samples can be realized, and the detection efficiency can be improved.

The application provides a recombinant microorganism, a construction method thereof and application of the recombinant microorganism in production of 3-hydroxybutyric acid or polyhydroxyalkanoate. The recombinant microorganism expresses a key enzyme or a key enzyme mutant for converting malonyl-CoA into acetoacetyl-CoA. The application recycles and reorients malonyl-CoA by introducing an acetoacetyl-CoA synthetase pathway, reduces waste of carbon sources, and improves conversion efficiency of raw materials to PHA or 3HB. The intermediates in the metabolic process are effectively utilized, and the production efficiency is improved. The application not only improves the utilization rate of common carbon sources such as glucose, but also enhances the metabolic capacity of other carbon sources (such as fatty acids), thereby improving the production potential of PHA.

The present disclosure relates to ganaxolone derivatives, as well as methods of using the compounds (e.g., for treating a disease or disorder), methods of making the compounds, and pharmaceutical compositions and kits containing the same. The present disclosure relates to ganaxolone derivatives. The compounds disclosed herein can be metabolized (e.g., in vivo, e.g., by metabolic processes) to ganaxolone. The compounds disclosed herein are also more soluble than ganaxolone in aqueous solutions, such as plasma, gastric fluid, or intestinal fluid. Also disclosed herein are methods of using the compounds, methods of making the compounds, and pharmaceutical compositions and kits containing them.

A class of multifunctional fluorescent dyes and their applications, belonging to the field of functional dye technology. This class of multifunctional dyes, as dual-color ratiometric pH fluorescent probes, introduces different fluorophores at the distal and proximal amino positions of amino acids to form a class of ratiometric fluorescent probes with FRET phenomenon, and is finally anchored to the target position through the active ester connected to the carboxyl group on the amino acid. This dual-color ratiometric pH fluorescent probe has the ability to quantitatively monitor pH and can monitor the dynamic changes and metabolic processes of the target object in situ. It can be used as a convenient strategy that is easy to use and does not require cell modification, which is conducive to use in a variety of scenarios and is expected to become a pH fluorescent sensing tool with application potential.

The application discloses an activated persulfate catalyst and a preparation method and application thereof, and relates to the technical field of catalysts.The catalyst is a bismuth-carbon intercalation catalyst in a chain structure, and the preparation method comprises the following steps: S1, taking a bismuth salt and an acyl benzene ligand as reaction substrates, and synthesizing Bi-MOFs through a hydrothermal reaction; and S2, calcining the Bi-MOFs at 500-700 DEG C under an inert gas atmosphere to obtain the bismuth-carbon intercalation catalyst.The bismuth-carbon intercalation catalyst activates persulfate through a non-radical direct electron transfer path, has higher activity and selectivity, can more effectively sterilize and disinfect specific structures and metabolic processes of target microorganisms, and improves the treatment efficiency; and can reduce the generation of by-products, effectively reduces the risk of the environment and the human body.

The invention discloses a high-yield cell-free protein synthesis system based on a cell crude extract and application of the high-yield cell-free protein synthesis system. The system comprises an escherichia coli extract, a DNA template, a buffer solution, an amino acid mixture, a nucleoside triphosphate mixture, an ATP regeneration system and antibiotics, the antibiotics are selected from compounds having inhibitory activity on metabolic processes except ribosome functions in prokaryotic microorganisms, including but not limited to one or more combinations of interfering DNA replication, DNA transcription, cell wall synthesis, energy metabolism, lipid synthesis or other non-translational related metabolic pathways. According to the method disclosed by the invention, the escherichia coli crude extract is used as a bacterial chassis, and non-essential metabolic pathways in a CFPS system are selectively inhibited by introducing different types of antibiotics, so that the yield of protein synthesis is increased.

The invention belongs to the technical field of drug toxicity prediction, and particularly relates to a drug toxicity prediction method based on metabolism guidance. The invention aims to guide a prodrug or a non-toxic substance through a metabolism method and consider the toxicity of the prodrug or the non-toxic substance in the metabolic process (for example, the prodrug is converted into an effective component in the metabolic process), so as to predict the potential toxicity of the prodrug or the non-toxic substance. By means of the guidance of metabolism, it is desirable to accurately predict whether or not a prodrug will generate toxicity in the body. In the technical scheme provided by the invention, molecules are learned by using different characterization modes for the molecular map, so that different features under different levels and different segmentation methods can be captured. Through the guidance of the metabolic diagram, the reactants can obtain product-related attributes for explaining the toxicity change generated in the metabolic process of the reactants.

The invention provides screening of protein-rich thermosynechococcus and a culture method and application of the protein-rich thermosynechococcus, and relates to the technical field of algae. The thermosynechococcus sp. LBV 12 is preserved in the China Center for Type Culture Collection on June 27, 2024, and the preservation number of the thermosynechococcus sp. LBV 12 is CCTCC (China Center for Type Culture Collection) NO: 20241317. According to the method, a Yunnan Tengchong hot sea water sample is separated and purified under the conditions that the temperature is 40-50 DEG C, the illumination intensity is 9000 Lux, the humidity is 45% and the light-dark ratio is 12 h: 12 h, and dominant algae strains are screened out according to the heat resistance degree and the growth condition. The thermosynechococcus shows a remarkable growth speed in an optimized culture medium, rapidly propagates and reaches high density, rich protein can be effectively synthesized and accumulated in the metabolic process, the content of essential amino acid is balanced, and the thermosynechococcus can serve as a high-protein source with great potential. And the product is rich in natural pigment phycocyanin, and has unique color and luster and oxidation resistance. Therefore, the thermosynechococcus has important application potential in the fields of food, feed, health care products and the like, and particularly provides powerful support for sustainable development of related industries in the aspect of developing natural products rich in nutrition.

The application discloses a method for dynamically regulating the content of smell and taste substances in the secondary metabolism process of streptomyces, and relates to the technical field of molecular biology. The method for dynamically regulating the content of smell and taste substances in the secondary metabolism process of streptomyces disclosed by the application determines a first promoter and an sgRNA sequence capable of targeting a first target gene affecting the synthesis of a target compound according to the types of microorganisms and the target compound; a first recombinant plasmid is constructed by using a molecular cloning method; meanwhile, a second promoter for starting the synthesis of the target compound and a gene sequence capable of encoding a Cas protein losing cutting activity are determined, and the two are connected to obtain a regulation unit; the linearized enzyme cutting product after double enzyme cutting of the first recombinant plasmid is connected with the regulation unit to obtain a time-sequential dynamic inhibition recombinant plasmid, and after the recombinant plasmid is introduced into streptomyces, a streptomyces strain with reduced content of smell and taste (geosmin) substances in the secondary metabolism process is obtained.

The application discloses a non-natural sugar and a synthesis method and application thereof. At room temperature, hydroxyl groups of an amino sugar are fully trimethylsilyl-protected, amino groups on the sugar are selectively exposed, and after coupling and conversion of the amino groups at room temperature, a non-natural sugar with orthogonal groups and full trimethylsilyl protection is obtained. The non-natural sugar with orthogonal groups and full trimethylsilyl protection is removed from trimethylsilyl protection groups, and a non-natural sugar without protection groups is obtained. The application takes into account the advantages of existing non-natural sugars, ensures that the non-natural sugars can be efficiently utilized by cells, effectively avoids S side reactions with cysteine in proteins in the metabolic process of the non-natural sugars, and simultaneously realizes efficient metabolic labeling. In a cell test, the use concentration of 1,6-bisacylated non-natural sugar is one order of magnitude lower than that of non-natural sugar without protection groups.