141 results about "Metabolic Process" patented technology

The invention relates to a method for identifying a disease microorganism-metabolite combined marker based on a host-flora co-metabolism model. The method comprises the following steps: processing microbiome data; setting diet constraint conditions; determining classification composition of intestinal microorganisms of each individual, mapping abundance of each microorganism to a human microorganism genome scale metabolism model, and constructing an individualized flora metabolism network model; carrying out metabolic flow simulation on the constructed flora metabolism network model; integrating the constructed flora metabolism network model with the host metabolism network model, and predicting the metabolic flow and metabolite change of the whole system; and establishing a disease classifier based on a random forest machine learning algorithm to identify the microorganism-metabolite combined marker. According to the method, the complex metabolic process between the human body and the intestinal flora is restored in a metabolic network modeling and metabolic flow simulation mode, the method has the non-invasive characteristic, the time and economic cost is remarkably reduced, and meanwhile, the method has a great advantage in accuracy.

The invention relates to the technical field of biological medicine, in particular to a dynamic fermentation tank control system for producing a diabetes intestinal microecological regulating preparation. The system comprises a fermentation tank sensing feedback control network establishment module, a fermentation tank growth earlier-stage nutrition supply control module, a fermentation tank growth later-stage adjustment control module and a fermentation tank metabolism process environmental condition control module, and can generate an embedded sensing feedback control network by installing a sensor and being connected with a central feedback control system. The sugar source supply quantity, the nitrogen source supply quantity, the temperature, the pH value, the dissolved oxygen concentration and the oxygen and carbon dioxide gas proportional flow corresponding to the fermentation tank are synchronously monitored in real time, so that supply cooperative dynamic control and fermentation growth cooperative dynamic control are carried out, and meanwhile, metabolism environment condition cooperative dynamic control is carried out; therefore, the cooperative control work of the microbial growth environment conditions is executed. By optimizing environment control in the fermentation process, efficient production of the intestinal microecology regulating preparation can be achieved.

The invention belongs to the technical field of biological medicines, and particularly relates to a pharmaceutical composition for preventing / treating Alzheimer's disease. According to the pharmaceutical composition based on the NPAFP protein provided by the invention, the synergistic effect of natural and artificially synthesized compounds is utilized, so that the pharmaceutical composition can play a role in a plurality of pathological levels, and the AD symptom is remarkably improved. The composition has the remarkable effects of resisting oxidation, resisting inflammation, promoting A beta removal, improving mitochondrial quality, reducing A beta 42 secretion and pathological states caused by Tau PFF and the like, and can protect neurons by adjusting the metabolic process. By screening and optimizing the compound with synergistic effect, the action mechanism of the compound is further defined, a novel safe and efficient potential solution is provided for AD treatment, and the compound has wide clinical application prospect. The medicine is high in safety, strong in specificity, simple and convenient in preparation method and suitable for mass production.

The invention discloses a satiety sugar-controlling fat-reducing composition and a preparation method and application thereof, the satiety sugar-controlling fat-reducing composition comprises hydrogel particles, a white kidney bean extract, a mulberry leaf extract, alpha-lipoic acid, L-carnitine, a lotus leaf extract, medium chain triglyceride, polyethylene glycol and sorbitol, and the composition is a tablet. The components are matched with one another to effectively adjust the leptin level, improve appetite, increase energy consumption, adjust the fat metabolism process, reduce fat accumulation, improve fat consumption, inhibit the activity of alpha-amylase, reduce the conversion of carbohydrates into fat, block heat and fat intake, promote gastrointestinal motility and enhance the immunity of the human body. The effects of remarkably reducing the fat content and the body weight are achieved.

The invention belongs to the field of leather making cleaning, and particularly relates to a leather bio-based degreasing agent and a preparation method thereof. The bio-based degreasing agent for leather comprises the following raw materials in percentage by weight: 18-22% of lipase, 38-42% of a bio-based surfactant, 0.1-0.2% of an enzyme activator, 1-3% of an enzyme stabilizer, 1-3% of a pH stabilizer and the balance of water. According to the leather bio-based degreasing agent disclosed by the invention, lipase and a bio-based surfactant (a metabolite with certain surface activity is secreted in a metabolic process when microorganisms are cultured under a certain condition) are organically combined, so that the lipase and the bio-based surfactant play a synergistic effect; and according to the detection result, the degreasing rate of the products obtained in the embodiments 1-3 is far higher than that of similar products sold in the market. In addition, the bio-based surfactant, lipase and other bio-based materials have great advantages in the aspects of carbon emission reduction, reproducibility and the like compared with a traditional petroleum-based surfactant, and popularization is more facilitated.

The invention provides a Burkholderia paradensis (Paraburkholderia caledonica) and an application of the Burkholderia paradonica (Paraburkholderia caledonica). The strain provided by the invention can realize green and efficient recovery of various rare earth raw materials. According to the present invention, the bacterial strain can produce a variety of organic acids and secondary metabolites having complexing properties during the metabolism process, and the electron transfer process on the surface of the microbial cell membrane provides important effects in the leaching of different rare earth raw materials;

The invention discloses a method for producing MK-7 through whole-cell regulation and control by using different time sequence type promoters, recombinant escherichia coli and application. According to the method, the time sequence type promoters are used for regulating and controlling expression of different key genes related to MK-7 synthesis. According to the invention, the time sequence promoter is used for whole-cell regulation and control to produce the vitamin MK-7 for the first time, dynamic regulation of the MK-7 is realized, and the production efficiency of the MK-7 can be remarkably improved by accurately regulating and controlling expression of key genes in a synthesis route of the MK-7 and optimizing an intracellular metabolic process. The promoter disclosed by the invention can regulate cell metabolism and enhance synthesis of MK-7 in different growth stages or under different environmental conditions by controlling the time and strength of gene expression. The MK-7 is produced by adopting the constructed recombinant escherichia coli obtained based on whole-cell regulation of the sequential promoter, and the yield of 14 g / L can be finally achieved in a 5L fermentation tank.

The invention relates to the technical field of microbial metabolism, in particular to a biological reagent production optimization method and system based on microbial metabolism characteristics. The method comprises the following steps: acquiring a biological reagent to produce a microbial strain, and determining the component proportion of a microbial culture medium and optimal microbial culture conditions; carrying out microorganism culture metabolism recording and metabolic flow path analysis on the biological reagent production microorganism strain, and simultaneously carrying out metabolic characteristic analysis to obtain metabolic quantitative characteristics of the biological reagent production microorganism; carrying out metabolic bottleneck node identification analysis on the metabolic flow path of the biological reagent production microorganisms to obtain metabolic bottleneck nodes of the biological reagent production microorganisms; and carrying out microbial production optimization on the corresponding biological reagent production microbial culture metabolism process, and carrying out production post-treatment and purification separation to obtain a biological reagent production optimization product. The production efficiency of the biological reagent can be remarkably improved, so that the stability and the consistency of the biological reagent product are ensured.

The invention relates to an ecological risk dynamic simulation evaluation and prediction early warning method based on a material metabolism process, and the method comprises the following steps: 1, ecological risk impact recognition: precisely analyzing the urban metabolism process through dynamic material flow analysis, and constructing an urban metabolism network model; step 2, ecological risk dynamic simulation evaluation: the risk impact of each metabolic subject is incorporated into an input-output model, and an urban ecological risk diffusion model is constructed; step 3, ecological risk prediction and early warning: constructing a prediction model of ecological risk impact indexes and influence factors based on an expandable random environmental influence evaluation model; the method has the advantages that key metabolic risk points are mined through risk impact recognition and quantification of all metabolic subjects, influence linkage conduction process simulation, risk index calculation and metabolic risk prediction and early warning, and stable operation of an urban agglomeration system is ensured; starting from the whole metabolic process of urban source supply and the like, the mechanism of metabolic risk is systematically explored.

The invention belongs to the field of escherichia coli metabolic processes, and particularly relates to a method for producing D-psicose from escherichia coli through glucose fermentation. Escherichia coli is used as a chassis host bacterium, a pgi gene, an SUMO-alsE fusion gene and an a6PP gene are subjected to overexpression at the same time, then a pfkA gene, a pfkB gene and a rpiA gene are knocked out in sequence, and a recombinant bacterium capable of producing D-psicose through glucose fermentation is obtained; the recombinant bacteria are used for fermentation, and glucose is converted to generate D-psicose.

The invention discloses a screening and expression platform construction method for non-essential gene loci of corynebacterium glutamicum, and belongs to the technical field of biology. The center sites CGLRS02940, CGLRS03085, CGLRS08555, CGLRS09020 and CGLRS13560 of the corynebacterium glutamicum identified by the invention have the remarkable advantages that when gene knockout or knockin operation is carried out, the sites do not interfere with the normal physiological metabolism process of strains, and stable expression of exogenous genes can be guaranteed; meanwhile, the gene editing efficiency is relatively high, and an editing target with high universality and high reliability is provided for genetic engineering modification of corynebacterium glutamicum.

The present invention relates to the field of biomedicine technology, and provides the use of an upregulator of ACOT2 in promoting liver regeneration and liver function recovery after partial hepatectomy. Specifically, it provides the use of an ACOT2 expression promoter in the preparation of a drug for promoting liver regeneration and liver function recovery after partial hepatectomy, as well as a pharmaceutical composition comprising the ACOT2 expression promoter. The present invention has developed new discoveries about the function of the ACOT2 gene. The increased expression of ACOT2 after hepatectomy has a certain protective effect on liver regeneration, or can serve as a potential marker for warning the degree of postoperative liver function damage and liver regeneration capacity, and a therapeutic target for promoting postoperative liver regeneration. By using the intervention method of the present invention, the decomposition and consumption of lipid droplets in the liver lipid metabolism process are targeted, thereby promoting the recovery of liver function and liver-to-body ratio.

The present invention provides a Bacillus tequilensis ZW-35 and its application, which relates to the technical field of microbial control. The Bacillus tequilensis ZW-35 is preserved in the China General Microbiological Culture Collection Center, and its taxonomic name is Bacillus tequilensis Bacillus tequilensis , and the preservation number is CGMCC No. 32878. The volatile substances in the metabolic process of Bacillus tequilensis ZW-35 can effectively inhibit the growth of Colletotrichum; and the volatile substances have high diffusion ability in air and soil pores. In the prevention and control of fruit diseases, the volatile substances can avoid direct contact with the fruits and will not leave residues on the fruit surface, thus avoiding the potential risks brought by direct contact.

The application discloses an Akkermansia muciniphila branched-chain amino acid metabolism gene nifJ and application thereof in colostrum IgA synthesis, and belongs to the technical field of genetic engineering. The application finds through in-vivo and in-vitro tests that the nifJ is a branched-chain amino acid metabolism gene of the Akkermansia muciniphila, regulates the metabolism process from branched-chain amino acids to branched-chain short-chain fatty acids, and plays a key role in the synthesis of maternal colostrum IgA. The research result of the application is expected to clarify the interaction relationship between the metabolic function of the Akkermansia muciniphila and a host, and reveal the action mechanism of the Akkermansia muciniphila in regulating the synthesis of maternal colostrum IgA, thereby providing a theoretical basis for improving the maternal colostrum IgA and further improving the maternal reproductive performance.

The invention provides a screening method of a targeted drug for inhibiting accumulation of trimethylamine and / or trimethylamine-N-oxide. The screening method comprises the following steps: determining an action target on a metabolic pathway of trimethylamine and / or trimethylamine-N-oxide; if the target is protein, preliminarily screening candidate components based on the three-dimensional structure of the target; the candidate component is validated to confirm the targeted drug. According to the method, large-scale screening of targeted drugs aiming at targets can be achieved, the targeted drugs inhibit accumulation of trimethylamine and / or trimethylamine-N-oxide in the body by influencing the metabolic pathway of trimethylamine and / or trimethylamine-N-oxide, side effects are small, and the normal metabolic process of the human body is prevented from being influenced.

The invention discloses a broad-spectrum plant-source disease-resistant excitant for rice blast and a preparation method thereof, and belongs to the technical field of rice blast prevention and control, in the broad-spectrum plant-source disease-resistant excitant, potassium glutamate serves as amino acid salt, not only provides a nitrogen source for plants, but also participates in the metabolic process in the plants and enhances the stress resistance of the plants. S-allyl-L-cysteine is a sulfur-containing amino acid derivative, has antioxidant and disease-resistant activity, and can stimulate a defense mechanism of plants and resist infection of magnaporthe oryzae. 2-piperidinecarboxylic acid has the effect of inhibiting growth of pathogenic bacteria and can directly interfere with cell wall synthesis of magnaporthe oryzae, so that diseases are relieved. Chitosan as a biological polysaccharide can induce plants to generate resistant substances, enhance the strength of plant cell walls, form a physical barrier and prevent invasion of pathogenic bacteria. Salicylic acid is an important signal molecule in a plant body and can activate systematic resistance of the plant and improve the overall disease resistance.

The utility model relates to the technical field of medical detection equipment, in particular to an in-vitro micro-tissue drug metabolism detection device. Comprising an incubation chamber, a printing terminal and an auxiliary assembly, a temperature control assembly is arranged at the top of the incubation chamber, the auxiliary assembly comprises a first conveyor, a second conveyor, a sorting robot, a sample pool and a detection component, the incubation chamber is used for incubating samples, the incubated samples are placed on the first conveyor to be conveyed, and after being detected by the detection component, the samples are sent to the printing terminal. After detection, samples can be rapidly placed on the second conveyor to be conveyed, finally, the samples are placed into the sample pools at different positions through the sorting robot according to feedback information of the control system, the printing terminal can print and output detection data, then the in-vivo microenvironment can be simulated, the in-vivo metabolic process of drugs can be accurately simulated, and the in-vivo microenvironment can be simulated. Moreover, preliminary detection and rapid transfer of samples can be realized, and the detection efficiency can be improved.

The present disclosure relates to ganaxolone derivatives, as well as methods of using the compounds (e.g., for treating a disease or disorder), methods of making the compounds, and pharmaceutical compositions and kits containing the same. The present disclosure relates to ganaxolone derivatives. The compounds disclosed herein can be metabolized (e.g., in vivo, e.g., by metabolic processes) to ganaxolone. The compounds disclosed herein are also more soluble than ganaxolone in aqueous solutions, such as plasma, gastric fluid, or intestinal fluid. Also disclosed herein are methods of using the compounds, methods of making the compounds, and pharmaceutical compositions and kits containing them.

The application discloses an activated persulfate catalyst and a preparation method and application thereof, and relates to the technical field of catalysts.The catalyst is a bismuth-carbon intercalation catalyst in a chain structure, and the preparation method comprises the following steps: S1, taking a bismuth salt and an acyl benzene ligand as reaction substrates, and synthesizing Bi-MOFs through a hydrothermal reaction; and S2, calcining the Bi-MOFs at 500-700 DEG C under an inert gas atmosphere to obtain the bismuth-carbon intercalation catalyst.The bismuth-carbon intercalation catalyst activates persulfate through a non-radical direct electron transfer path, has higher activity and selectivity, can more effectively sterilize and disinfect specific structures and metabolic processes of target microorganisms, and improves the treatment efficiency; and can reduce the generation of by-products, effectively reduces the risk of the environment and the human body.

The invention discloses a high-yield cell-free protein synthesis system based on a cell crude extract and application of the high-yield cell-free protein synthesis system. The system comprises an escherichia coli extract, a DNA template, a buffer solution, an amino acid mixture, a nucleoside triphosphate mixture, an ATP regeneration system and antibiotics, the antibiotics are selected from compounds having inhibitory activity on metabolic processes except ribosome functions in prokaryotic microorganisms, including but not limited to one or more combinations of interfering DNA replication, DNA transcription, cell wall synthesis, energy metabolism, lipid synthesis or other non-translational related metabolic pathways. According to the method disclosed by the invention, the escherichia coli crude extract is used as a bacterial chassis, and non-essential metabolic pathways in a CFPS system are selectively inhibited by introducing different types of antibiotics, so that the yield of protein synthesis is increased.

The invention belongs to the technical field of drug toxicity prediction, and particularly relates to a drug toxicity prediction method based on metabolism guidance. The invention aims to guide a prodrug or a non-toxic substance through a metabolism method and consider the toxicity of the prodrug or the non-toxic substance in the metabolic process (for example, the prodrug is converted into an effective component in the metabolic process), so as to predict the potential toxicity of the prodrug or the non-toxic substance. By means of the guidance of metabolism, it is desirable to accurately predict whether or not a prodrug will generate toxicity in the body. In the technical scheme provided by the invention, molecules are learned by using different characterization modes for the molecular map, so that different features under different levels and different segmentation methods can be captured. Through the guidance of the metabolic diagram, the reactants can obtain product-related attributes for explaining the toxicity change generated in the metabolic process of the reactants.

The application discloses a method for dynamically regulating the content of smell and taste substances in the secondary metabolism process of streptomyces, and relates to the technical field of molecular biology. The method for dynamically regulating the content of smell and taste substances in the secondary metabolism process of streptomyces disclosed by the application determines a first promoter and an sgRNA sequence capable of targeting a first target gene affecting the synthesis of a target compound according to the types of microorganisms and the target compound; a first recombinant plasmid is constructed by using a molecular cloning method; meanwhile, a second promoter for starting the synthesis of the target compound and a gene sequence capable of encoding a Cas protein losing cutting activity are determined, and the two are connected to obtain a regulation unit; the linearized enzyme cutting product after double enzyme cutting of the first recombinant plasmid is connected with the regulation unit to obtain a time-sequential dynamic inhibition recombinant plasmid, and after the recombinant plasmid is introduced into streptomyces, a streptomyces strain with reduced content of smell and taste (geosmin) substances in the secondary metabolism process is obtained.

The application discloses a non-natural sugar and a synthesis method and application thereof. At room temperature, hydroxyl groups of an amino sugar are fully trimethylsilyl-protected, amino groups on the sugar are selectively exposed, and after coupling and conversion of the amino groups at room temperature, a non-natural sugar with orthogonal groups and full trimethylsilyl protection is obtained. The non-natural sugar with orthogonal groups and full trimethylsilyl protection is removed from trimethylsilyl protection groups, and a non-natural sugar without protection groups is obtained. The application takes into account the advantages of existing non-natural sugars, ensures that the non-natural sugars can be efficiently utilized by cells, effectively avoids S side reactions with cysteine in proteins in the metabolic process of the non-natural sugars, and simultaneously realizes efficient metabolic labeling. In a cell test, the use concentration of 1,6-bisacylated non-natural sugar is one order of magnitude lower than that of non-natural sugar without protection groups.

The invention discloses a multi-gene tandem recombinant strain as well as a construction method and application thereof. The invention also discloses a method for synthesizing lycopene based on an isopentenol utilization approach. The strain comprises a recombinant strain of seven key genes, namely mtipk, thim, ispA, idi, crtE, crttheB and crtI, for synthesizing lycopene in an IUP (Inhibitory Urinary Protein) way. According to the invention, the isopentenol is introduced into the escherichia coli in a way to synthesize the lycopene; according to the approach, only two-step phosphorylation reaction is used for replacing the original 2-C-methyl-D-erythritol-4-phosphoric acid approach and mevalonic acid approach which need more than seven steps to synthesize precusor substances pentadienyl pyrophosphoric acid and dimethyl allyl pyrophosphate ester of lycopene, so that the metabolic pathway is greatly shortened, and competition with central carbon metabolism in cells is avoided; the toxicity caused by accumulation of intermediate products in the metabolic process is reduced, and lycopene can be efficiently synthesized by using a cheap substrate.

Exhaled breath contains trace levels of volatile organic compounds (VOCs) that can reveal information about metabolic processes or pathogens in the body. These molecules can be used for medical diagnosis, but capturing and accurately measuring them is a significant challenge in chemical separations. A highly selective nanoporous metal-organic framework (MOF) sorbent can be used to capture target molecules from a breath sample and pre-concentrate them for use in a detector. As examples, a series of Zr-based MOFs, MOF-818, PCN-777, and UiO-66, each recover around 40-60% of the targets (with the exception of acetaldehyde) at up to 95% relative humidity. In addition, a series of hydrophobic MOFs modified with fluorine functional groups were synthesized that have increased affinity for VOCs in the presence of humidity. In particular, fluorinated MOF-808 derivatives demonstrated substantial increase in the retention of target VOCs adsorbed from a humid gas stream with ppm level VOC concentration.

The present invention relates to the field of biomedicine technology, and in particular to a dynamic fermentation tank control system for the production of diabetic intestinal microecological regulation preparations. The system includes a fermentation tank sensor feedback control network establishment module, a fermentation tank growth early stage nutrient supply control module, a fermentation tank growth late stage regulation control module, and a fermentation tank metabolic process environmental condition control module. An embedded sensor feedback control network can be generated by installing sensors and connecting to a central feedback control system, and synchronously monitoring the sugar source supply, nitrogen source supply, temperature, pH value, dissolved oxygen concentration, and oxygen and carbon dioxide gas ratio flow rate corresponding to the fermentation tank in real time to perform supply coordinated dynamic control and fermentation growth coordinated dynamic control, while performing metabolic environmental condition coordinated dynamic control to perform microbial growth environmental condition coordinated control work. The present invention can achieve efficient production of intestinal microecological regulation preparations by optimizing environmental control during the fermentation process.

The invention belongs to the technical field of analysis and detection, and particularly relates to a preparation method and application of a matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry plant sample, the preparation method of the plant sample created by the invention can separate leaf inner epidermis and mesophyll tissue, and the leaf inner epidermis and mesophyll tissue are utilized to research metabolites in leaves. After the inner epidermis and mesophyll of the leaf are separated, not only can organic acid, small molecular compounds and macromolecular substances in the leaf be detected, but also the change of the content of the organic acid can be revealed. According to the MALDI mass spectrometry technology, assimilation of NH4 < + > / NO3 <-> in leaf inner epidermis or mesophyll tissue can be monitored in an imaging mode, so that the metabolic process of nitrogen is visualized; in the nitrogen metabolism process, NH4 < + > is directly used for synthesizing amino acid, NO3 <-> is reduced into NO2 <->, and NO2 <-> is assimilated into amino acid and derivatives thereof. The preparation method provided by the invention has unique advantages, and is beneficial to deeply understanding the metabolic process and biological functions of plants.

The invention provides a covalent complex of human milk oligosaccharide and a fluorescent reagent and a preparation method of the covalent complex, and belongs to the field of organic chemistry. The application of the human milk oligosaccharide-cyanine dye Cy7 covalent complex provided by the invention in monitoring the metabolic distribution of the human milk oligosaccharide-cyanine dye Cy7 covalent complex in internal tissues and organs through fluorescence imaging has a good tracing effect, and can dynamically trace the metabolic process of human milk oligosaccharide in vivo at different time points; compared with a traditional liquid chromatography-mass spectrometry in-vitro drug metabolism detection method, the fluorescence tracing method has the advantage that the in-vivo metabolism condition of the human milk oligosaccharide can be accurately, intuitively and efficiently monitored.

The present application relates to novel compounds that are prodrugs of brexpiprazole. The novel compounds of the present invention, upon administration to a subject, undergo chemical conversion by one or more metabolic processes to release the active agent in vivo. Brexpiprazole sold by Rexulti (registered trademark) is an atypical antipsychotic drug, is suitable for being used as an adjuvant therapy of an antidepressant drug, and is used for treating major depressive disorder (MDD) of adults and schizophrenia of adults and children patients over 13 years old. # imgabs0 #

A process is disclosed for production of a polyhydroxyalkanoate that includes depolymerization of a post-consumer polyhydroxyalkanoate and utilization of the hydroxyalkanoate monomer thus produced as a carbon source for a microorganism capable of production of a polyhydroxyalkanoate. Methods can be utilized for true cyclic use of polyhydroxyalkanoates including polyhydroxybutyrates. Various aspects are described including simultaneous depolymerization and polymer production, utilization of purified depolymerase enzymes and / or microorganisms that express a depolymerase in conjunction with a microorganism that produces polymer, utilization of microorganisms that produce both a depolymerase and a new polymer, and utilization of genetically modified organisms to produce natural or modified depolymerase enzymes.