225 results about "Enterobacter sp" patented technology

The invention provides compositions and methods for engineering E. coli or other host production bacterial strains to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.

The invention discloses a bacterial strain b capable of resisting the growth of heavy metal Cd and reducing sulfate, and a bacterial strain capable of resisting the growth of the heavy metal Cd and solubilizing phosphate. The classification and name of the bacterial strain b and the bacterial strain are respectively enterbacter ludwigii SRB-2-5U-2 and pseudochrobactrum saccharolyticum LB-4-4-1c, which are already preserved in the Common Microbe Center of the China Committee for Culture Collection of Microorganisms. The bacterial strains disclosed by the invention can be used for simultaneously remediating the surface soil, deep soil and rhizosphere soil of a plant, reducing the pollution of Cd to surface water and underground water, and also improving the activity and fertility of the soil.

The invention relates to a micro-capsulated suspended microbial seed coating agent and a preparation method thereof. The micro-capsulated suspended microbial seed coating agent comprises a functional bacteria microcapsule and auxiliaries, wherein the functional bacteria microcapsule is prepared from wall materials such as sodium alginate and gelatin by virtue of an endogenous emulsion process, the grain size of the microcapsule is 20-60 mu m, the content of functional bacteria is not lower than 10<9>cfu/g, and the embedding rate is over 80%. Functional microbes are selected from two or more of pseudomonas, bacillus subtilis, bacillus amyloliquefaciens, enterobacter cloacae, raoultella planticola and bacillus atrophaeus. The auxiliaries comprise one or more of a film-forming agent, a dispersant, a tackifier, an anti-freezing agent and a coloring agent. The prepared functional bacteria microcapsule is uniformly mixed with the auxiliaries to obtain a finished product. The product has the advantages of good film-forming performances, thallus persistence, high survivability, good slow-controlled release, strong application stability, environment friendliness, convenience in use, and the like, can be used for promoting seed germination and seedling growth of cottons, soya beans, wheat and potatoes, and the like, controlling the diseases at the seedling stage, improving stress resistance, and the like.

This invention relates to a fusion protein used for preventing aftosa, its preparation method and application. This fusion protein contains O type foot-and-mouth disease virus main cytomembrane protein VP1 epitope, colibacillus thermolability toxin B subunit, thymus derived cell epitope and purification label.

The invention belongs to biology technology field, which concretely refers to controlled release tissue factor path inhibitor (LTFPI) and the manufacturing method, and the application. The invention analyzes and experiments the biology information science and structure molecular biology science of tissue factor path inhibitor (TFPI) and its acceptor low density lipoprotein acceptor correspondent protein (LRP), it ascertains the part where the TFPI carboxy end combines with LRP and is eliminated. It designs TFPI carboxy end mutant, which is recombined with primary nucleus or eukarya expressing carrier after constructing LTFPI gene through PCR location, it converts bacillus coli or bici yeast, sifts the high expression project fungus. The primary project fungus are yeasted and expanded, crushed, then they are centrifugated and collects the inclusion body, purifies the LTFPI through molecular sift and ion interchanging two-step method; the eukarya project fungus are carried on with two-step purifying directly. The half-life are prolonged, it has good pour-depressant function.

The invention relates to a pig breeding and breathing complex virus (PPRSV) and pig 2-type loop virus (PCV 2) series gene recombination adenovirus and vaccine in the field of high and new biotechnology. It uses PCR technology to clone the Cap protein gene into the plasmid carrier pShuttle-CMV-GP5 by open type reading rule and transforms tobacillus coli BJ5183strain with cage carrier pAdEasy-1 to capture the recombination plasmid; it uses recombination plasmid HEK293-A cell to capture recombination adenovirus and purifies it to express the recombination adenovirus rAd-Cap-GP5 of PRRSV GP5protein and PCV-2 Cap protein.

The invention discloses sulfate reducing bacteria with tolerance on arsenic. The sulfate reducing bacteria are named as enterobacteria (enterobacter sp.) taihuN3 in classification, wherein the bacterial strain is preserved in General Microbiology Centre of China Committee for Culture Collection of Microorganisms; the preservation number is 23 Nov 2012; and the preservation number is CGMCC No.6886. Application of the sulfate reducing bacteria with tolerance on arsenic in wastewater treatment is also disclosed by the invention. The bacterial strain disclosed by the invention is wide in utilizable carbon source range, easy to cultivate, strong in growth metabolic activity, and high in sulphate conversion efficiency. The bacterial strain disclosed by the invention can normally grow when the concentration is 10-1000mg/LAs(V); and the percent reduction of the bacterial strain disclosed by the invention under 200mg/LAs(V) of sulfate achieves 50%. The sulfate reducing bacteria can be used for processing high-concentration arsenic wastewater, and has good application prospect.

The invention relates to an expression, purification, crystallization method, structure elucidation and the field of application technology of forulic acid decarboxylase, which belongs to the field of molecular biology and microbiology application. The invention obtains the coding area sequence of the key enzyme forulic acid decarboxylase which decomposes forulic acid to generate 4-vinyl guaiacol in Enterobacter sp.Px6-4 by the gene clone method, expresses the enzyme in a large amount in the colon bacillus ( E.coli BL21 ) by the heterogenous expressing technology, obtains the forulic acid decarboxylase pure products by the purification method and the forulic acid decarboxylase protein crystallization by the hanging-drop crystallization process, and grasps the structure and active centre of the enzyme. The invention can obtain a large amount of highly active forulic acid decarboxylase, and provide theoretical guidance for reconstructing engineering bacteria and improving the output of 4-vinyl guaiacol by analyzing the active centre of the enzyme, thus having good potential application.

An deactivated multi-bacterium vaccine for preventing the mastitis of milk cow is composed of Staphylococcus aureus, agalactic streptococcus, milk-stopping streptococcus, breast streptococcus, colibacillus and aluminum hydroxide gel. Its preparing process includes such steps as choosing the pathogenic bacterial strain of said mastitis, preparing seed liquid and reproduction liquid, purity checking, deactivating, preparing vaccine, and discriminating.

The present invention relates to gene chip for detecting several kinds of common bacterial pathogens, and the gene chip includes solid carrier and oligonucleotide probe fixed on the carrier. The chip has up to 200 low density distributed sample points. The oligonucleotide detecting probe includes DNA' s selected from nucleotide sequences corresponding to the genomes of nine kinds of common bacterial pathogens, including Staphylococcus aureus, Staphylococcus epidermidis, bacillus pyocyaneus, colibacillus, etc. The said chip together with sample treating reagent, hybridizing reagent, color reagent and the specification constitutes the detection kit. The present invention has high detection efficiency.

The invention discloses a heavy metal-tolerant bacterial strain Enterobacter sp.T2 and application thereof. The collection number of the heavy metal-tolerant bacterial strain Enterobacter sp.T2 is CGMCCNo.4281. The heavy metal-tolerant bacterial strain Enterobacter sp.T2 can tolerate various heavy metals such as Pb<2+>, Cr<6+>, Mn<2+>, Zn<2+>, Cu<2+>, Ni<2+>, Cd<2+>, Co<2+>, Ag<+>, Hg<2+> and the like, in particular, the Pb<2+> tolerance concentration and the Mn<2+> tolerance concentration are more than 2,000 mg/L and 1,000 mg/L respectively. A heave metal-removed adsorbent is prepared by activating, culturing, collecting, washing and drying the bacterial strains and the maximum adsorption quantity of the heavy metal Pb<2+> can reach 81.22 mg/g.

The invention provides a preparation method of hyaluronic acid. The method comprises the following steps: transforming a recombinant expression vector into the colibacillus to obtain engineering colibacillus by constructing the recombinant expression vector of which hyaluronic acid synzyme gene Has and uridine diphosphate glucose dehydrogenase gene are jointly expressed in the colibacillus; and fermenting the engineering colibacillus to obtain the hyaluronic acid. After the method is used, the production yield of the hyaluronic acid achieves 2.5g/L. compared with the prior art, the yield of the highest HA (hyaluronic acid) obtained by gram positive chained coccus HA synthetic gene spHas expressed in the colibacillus is approximately improved by 10 times.

A reagent kit based on recombinant antigen for detecting Toxoplasma is prepared through taking 542-1218 fragment (t SAG1) from the primary surface antigen gene SAG1 of Toxoplasma, subcloning it to soluble expression carrier pET32a(+), transferring it to colibacillus, configuring engineering bacterium pET32a-tSAG1/BL21, IPTG induced efficient expression, ultrasonic splitting to obtain supernatant, purifying by Ni-NTA and Sephadex-G75, and coating the microholes on ELISA plate. Its test paper can also be prepared by same way.

The invention belongs to the field of biological repair of environmental pollution, and particularly relates to a 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium SE08, and a screening method and application thereof. The 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium SE08 is Enterobacter sp. of which the collection number is CGMCC No.7016 and the collection date is 2012.12.18; and the 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium SE08 is collected at China General Microbiological Culture Collection Center. The invention also discloses a screening method and application of the 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium SE08. The 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium SE08 is obtained by primarily screening in 2-methyl-4-chlorophenoxyacetic acid heavily-polluted soil in the tobacco growing area in Chenzhou City of Hunan Province, rescreening and separation purification; and the degrading bacterium can effectively degrade 2-methyl-4-chlorophenoxyacetic acid residues in the environment, and is applicable to biological repair in 2-methyl-4-chlorophenoxyacetic acid polluted soil and water.

An antimicrobial composition and a method for administering the antimicrobial composition through the water or feed of livestock, wherein the antimicrobial composition is made up of lysozyme and various other agents that act synergistically with lysozyme, such as, dried egg powder, albumen, a sequestering agent and/or a lantibiotic. The composition is used to inhibit the growth of, and diseases and epidemiological significant effects caused by, Clostridium perfinigens, E, coli and Salmonella, in the gut of livestock. More particularly, the antimicrobial composition and method relate to a feed additive that can be administered to poultry and/or swine through their feed. The use of such a feed additive may also inhibit other enteric pathogens that may be present in the gut of livestock.

The invention discloses Enterobacter mori, and a biotransformation method for a fermented wheat bran extract used for cigarette and an application, which belongs to the biology technical field and cigarette flavor technical field. The invention provides a strain of Enterobacter mori VL4-3CCTCC M2012020. The biotransformation method for fermented wheat bran extract used for cigarette by the Enterobacter mori comprises the following steps: 1)backflow extraction; 2)inclined plane culture; 3) seed culture solution preparation; 4) fermentation; and 5) extract preparation. The invention also provides an application of the Enterobacter mori in biotransformation to prepare the fermented wheat bran extract used for cigarette. According to the invention, the process is simple, environmental protection and harmlessness performances are provided, the raw material cost is low, the product is natural, and the fermented wheat bran extract has industrial prospect. The fermented wheat bran extract for cigarette can be directly used for perfuming on the cigarette and coordinating with flue gas, the fermented wheat bran extract has thick and sweet fragrance, thereby the flue gas is softened, the fragrance is enriched, and the adsorption can be improved.

The invention belongs to the technical fields of molecular biological techniques and clinical tests, and particularly discloses a nosocomial infection multiple causative agent parallel detection gene chip and a preparation method and application thereof. The gene chip can be used for rapidly and sensitively detecting fourteen medical treatment infection typical causative agents such as acinetobacter baumannii, enterobacter cloacae, enterococcus faecalis and the like by using one reaction, and the detection data of coinfection of yeast and bacterium which cannot be provided by a traditional amplification method is offered. Furthermore, the gene chip also comprises a methicillin resistance target spot, thus realizing further detection of staphy lococcus infection, therefore, a clinician can realize which causative agent a patient is infected in one or two hours, and realize whether the patient has drug resistance, so as to decide whether the patient needs antibiotics or which antibiotics is needed, so that the gene chip has a greater clinical guiding significance.

A bacteria suppressing collagen-DNA-Ag complex is prepared from collagen, DNA and AgNO3 solution through ion exchange, dialysis, degassing, shaping, freezing, drying and sterilizing. It can be used to suppress staphylococcus aureus, Rseudomonas aeruginosa and colibacillus, and treat burn and scald.

Low levels of quaternary ammonium compounds are effective antimicrobial agents in drinking water and potentiate the antimicrobial power of organic acids used as antimicrobials for such purposes. The method is effective against both Gram (−) and Gram (+) bacteria, including but not limited to, Salmonella sp., E. coli, Campylobacter sp. as Staphylococcus sp. and Listeria sp. The combination of quaternary ammonium compounds and one or more organic acids can also be effectively used as antimicrobial washes for fruits, vegetables, meat, and animal carcasses.

The invention discloses kappa-carrageenase, a gene encoding the same and an application of kappa-carrageenase. The cloned kappa-carrageenase precursor gene car 30 of P.carrageenovora ASY5 is transformed into E. coli, a recombinant bacterium producing recombinant kappa-carrageenase is obtained, and the suitable temperature and pH of the recombinant kappa-carrageenase are 45 DEG C and 6.5; after heat preservation at 40 DEG C for 30 min, the remnant enzyme activity is 80.0% or higher, the recombinant kappa-carrageenase is treated for 60 min in a buffer solution with pH being 6.5-7.5, and the enzyme activity can still remain 50.0% or higher. The recombinant vector containing the kappa-carrageenase gene car 30 is constructed, heterologous expression is realized, and a good foundation is provided for industrialized production and application of the enzyme. The expressed kappa-carrageenase has a better degradation effect on kappa-carrageenan, kappa-carrageenan is degraded into disaccharide, tetrasccharide and the like, and the kappa-carrageenase can be applied to preparation of antibacterial agents, antiviral agents, immunomodulators, antioxidants and the like.

The invention discloses an Enterobacter sp. strain and application thereof. The strain is Enterobacter sp. FL which is collected by China General Microbiological Culture Collection Center (CGMCC) on January 12th, 2016; and the collection number is CGMCC No.11993. The Enterobacter sp. FL can be applied to the field of nitrogenous organic wastewater treatment, and can remove nitrate nitrogen in wastewater under aerobic conditions under the action of denitrification. The Enterobacter sp. secretes and generates extracellular polymers mainly composed of proteins, polysaccharides and the like in the denitrification process. The polymers can initiate spontaneous aggregation and precipitation of bacterial cells and implement bacterium-water separation. The strain has the aerobic denitrification capacity, can spontaneously aggregate to form a precipitate in the denitrification process, and has the characteristics of aggregation and spontaneous precipitation, so that a reactor using the strain as functional bacteria is established in the later period, thereby implementing aerobic denitrification and enabling the acquisition of clear effluent water by sludge-water separation. The strain has wide application prospects and favorable social and environmental benefits.