80 results about "Enterobacter sp" patented technology

The invention discloses a method for improving the solubility and thermal stability of sweet protein, and belongs to the technical field of biosynthesis, the method comprises the following steps: constructing a gene tandem recombinant plasmid containing a monellin x-3C-sfGFP-3C-monellin y expression cassette, x is greater than or equal to 1, y is greater than or equal to 1, 5 is greater than or equal to x + y is greater than or equal to 3, a 3C protease recognition sequence is also inserted between adjacent copies of monellin, and each monellin is connected with a purification tag; the gene tandem recombinant plasmid is transformed into escherichia coli and inducible expression is carried out, thalli are split and purified to obtain fusion protein, 3C protease is used for enzyme digestion, and the target protein is obtained after re-purification. The total protein yield and the solubility proportion are remarkably improved by adopting a series construction mode of plasmids, meanwhile, the stability of monellin is effectively improved, the purification step is simpler and more convenient, and the method is suitable for industrial production.

The present disclosure provides a recombinant Escherichia coli strain modified by metabolic engineering means and a method for producing L-homoserine by using the same. The strain, designated as Escherichia coli having a strain number of 13-XA, is deposited in China General Microbiological Culture Collection Center (CGMCC) with an accession number of CGMCC No. 25099, dated Jun. 16, 2022. With respect to the chromosome DNA thereof, one or more genes associated with fatty acid metabolism are knocked out or attenuated, and / or a promoter is replaced for enhancement; one or more genes associated with the L-homoserine metabolic pathway are knocked out or attenuated, and / or one or more genes associated with the L-homoserine metabolic pathway are overexpressed or enhanced, and / or one or more genes associated with the L-homoserine metabolic pathway are mutated.

The invention discloses a conservative B cell epitope peptide of goose parvovirus VP3 protein, a nucleic acid molecule, a recombinant vector and application thereof. Conservative B cell epitope EW12 is inserted into F18 Escherichia coli Fod fimbriae, inert carrier bacteria S9H are introduced, recombinant bacteria capable of functionally exhibiting and expressing the EW12 conservative B cell epitope on the surface of the bacteria are obtained, the recombinant bacteria can specifically generate specific binding reaction with gosling plague egg yolk antibody and gosling plague vaccine immune serum, and the gosling plague egg yolk antibody and the gosling plague vaccine immune serum can be specifically combined with the gosling plague egg yolk antibody and the gosling plague vaccine immune serum. The method has the advantages that macroscopic agglutination particles are generated, cross agglutination reaction with other pathogen positive serum and healthy goose serum is avoided, the method is good in sensitivity, and the specific antibody can be detected in the 5th day after immunization at the earliest. A plate agglutination test directly mediated by the goose parvovirus VP3 protein conservative B cell epitope peptide EW12 has the advantages of specificity, sensitivity, convenience in operation and suitability for on-site large-scale rapid detection, and is expected to provide a new technical means for diagnosis, prevention and control and purification of GPV infection.

The invention relates to a microbial agent for degrading COD (Chemical Oxygen Demand) in DBP (Dibutyl Phthalate) wastewater as well as a preparation process and application of the microbial agent. The microbial agent is prepared from the following active components in parts by weight: 88 to 91 parts of pseudomonas, 5.2 to 5.6 parts of enterobacter and 0.05 to 0.11 part of alcaligenes. According to the application, a plurality of microbial strains are formed in the microbial liquid which is subjected to mixed domestication culture of the DBP wastewater, the anaerobic sludge, the hydrolytic acidification bacteria and the nutritive salt, and a plurality of microbial strains in the microbial agent which is obtained by mixing the microbial liquid after bacteria screening and expanding culture have a synergistic effect, so that the microbial agent has the performance of efficiently degrading COD in the DBP wastewater in an environment-friendly manner.

The invention discloses a multifunctional composite flora capable of degrading atrazine at low temperature and increasing phosphorus as well as a preparation method and application of the multifunctional composite flora, and relates to the technical field of farmland soil pollution microbial remediation. The invention aims to solve the problem of poor atrazine pollution remediation effect of a single strain in a low-temperature environment. The preparation method comprises the following steps: mixing a simple pure bacillus C1 bacterial solution, an enterobacter (Enterobacter sp.) P1 bacterial solution and an Acinetobacter lwoffii DNS32 bacterial solution, so as to obtain the multifunctional composite flora capable of degrading the atrazine at the low temperature and increasing the phosphorus, which is disclosed by the invention, and the preparation method comprises the following steps: mixing the bacterial solution of the simple pure bacillus, the bacterial solution of the enterobacter (Enterobacter sp.) P1 bacterial solution and the bacterial solution of the Acinetobacter lwoffii DNS32 bacterial solution, so as to obtain the multifunctional composite flora capable of degrading the atrazine at the low temperature and increasing the phosphorus. The invention can obtain the multifunctional composite flora capable of degrading atrazine at low temperature and increasing phosphorus as well as the preparation method and the application of the multifunctional composite flora.

Primers for of amplifying DNA from shiga toxin producing E. coli (STEC); methods of amplifying STEC DNA; and methods of detecting STEC.

Antimicrobial eyewear features frames infused with or coated with antimicrobial additives, thereby offering continuous protection by actively destroying and inhibiting the growth of bacteria, fungi, parasites, and some viruses. The lenses may also have an antibacterial coating that destroys and inhibits bacterial growth. In some examples, the lenses are treated with a specialized strengthening liquid, wherein the liquid contains nano components that release heavy metal ions capable of invading the cell walls of bacteria, leading to bacterial elimination, destroying DNA molecules and proteases within the bacterial cells, denature viral proteins, break DNA chains, and result in cell death. Additionally, the strengthening liquid reduces dehydrogenase activity and interacts with various protein groups within the cell, thereby reducing the activity of these groups and effectively inhibiting the growth of E. coli and other bacteria on the lens surface.

The application discloses a soil enterobacter capable of efficiently degrading polypropylene and application thereof. The strain is FJNUIM-S2, which is classified and named as soil enterobacter (Enterobacter agglomerans) Enterobacter soli ), is preserved in the China General Microbiological Culture Collection Center (CGMCC), and is preserved on October 17, 2024, with a preservation number of CGMCC No. 32241 and a preservation address of No. 3, Institute of Microbiology, Chinese Academy of Sciences, Xiliujiaobei, Chaoyang District, Beijing. The strain FJNUIM-S2 of the application can grow with polypropylene as the only carbon source, has good polypropylene degradation characteristics, provides a microbial resource for bioremediation of waste polypropylene in the environment, and has a wide application prospect.

Recombinant E. coli strains and synthetic protein sequence designs are leveraged for production of disordered polypeptides such as spidroins and elastin-like peptides (ELPs). These disordered polypeptides, the high-titer production of which has proven difficult, include repeating structural motifs from a small selection of amino acid residues, resulting in lack of well-defined tertiary and quaternary structure. The recombinant E. coli include expression vectors with genes encoding for the disordered polypeptide product. Expression of these genes is controlled by a promoter that downregulates and substantially inhibits basal expression in the recombinant bacteria. Further, the recombinant bacteria include mutations to one or more stress-response genes from wild-type E. coli, such as yggw, yedv, yedw, yedy, spec, speb, uspc, hcha, loip, mltc, envz, ompr, yhgf, or hupb. The recombinant E. coli enable production of high titers of disordered protein product while minimizing the toxic effects thereof on the host.

The application belongs to the field of enzyme engineering and biotechnology, and discloses a 4-hydroxybenzoic acid hydroxylase mutant and application thereof in synthesis of gallic acid or a salt thereof. The 4-hydroxybenzoic acid hydroxylase mutant is obtained through directional evolution modification and high-throughput screening, and has an increased protocatechuate hydroxylase activity by more than 4 times. The 4-hydroxybenzoic acid hydroxylase mutant is expressed by using E. coli, and the obtained recombinant E. coli can efficiently catalyze the conversion of protocatechuate into gallic acid. The 4-hydroxybenzoic acid hydroxylase mutant is expressed in the recombinant E. coli for producing protocatechuate, and the obtained recombinant E. coli can de novo synthesize gallic acid from glucose as a substrate, and the yield reaches 58.65 g / L, and the residual amount of protocatechuate is only 0.33 g / L. The application solves the problem of a large amount of residual protocatechuate in the fermentation process of the existing strains, and can provide an efficient enzyme element for the creation of gallic acid industrial strains.

The invention provides an escherichia coli engineering bacterium for high yield of yellow cochineic acid and a construction method of the escherichia coli engineering bacterium, and a modified chassis cell escherichia coli BAP1-pflB is obtained by performing gene knockout on a pflB gene in an escherichia coli thallus BAP1. And the constructed recombinant plasmid pET30a-antDEFBG + zhuIJ is electrically transformed into escherichia coli BAP1-pflB, so that the engineering bacterium BAP1-pflB-PFK capable of producing the yellow cochineic acid is obtained. Through HPLC (High Performance Liquid Chromatography) detection, after the gene pflB is knocked out, the yield of the yellow cochineic acid is obviously increased. Yellow carmine acid synthesized by the escherichia coli engineering bacteria is a high-grade precursor of carmine acid with high commercial value, and plays an important role in the fields of textiles, skin care products and the like.

The invention belongs to the technical field of microbial treatment, and particularly relates to enterobacter hormaechei and application thereof.The enterobacter hormaechei has the function of efficiently dissolving out high-valence metal cations such as Fe < 2 + > / 3 < + >, Ca < 2 + > and Mg < 2 + > from iron tailings, the functional strain is used for treating coastal solonchak, dissolved-out free-valence metal ions can accurately target Na < + > adsorbed by soil colloid, and by means of the ion exchange effect, the soil colloid can be accurately adsorbed by the dissolved free-valence metal ions; na < + > is desorbed from a saline soil body and discharged out of plough layer soil, the problems that the coastal saline soil is high in salinity and single in component can be effectively solved, and an advanced and reliable technical means is provided for ecological restoration and agricultural utilization of the coastal saline soil.

The application discloses a CMV virus-like particle for producing a VLP recombinant vaccine and a preparation method thereof, the CMV virus-like particle is obtained by cloning a C-terminal G4SLPETG modified plant virus cucumber mosaic virus CMV capsid gene into a prokaryotic expression vector to obtain a recombinant expression vector, the recombinant expression vector is transfected into E. coli BL21 (DE3), and expression is obtained through the recombinant E. coli BL21 (DE3); and the amino acid sequence of the C-terminal G4SLPETG modified CMV is SEQ ID NO. 1. Test proves that the recombinant strain constructed by the application is stable to expression of an exogenous protein. The recombinant protein expressed by the application is used for preparing a virus-like particle for antigen coupling, the coupling efficiency is high, the antigen purity is high, the safety is good, the virus-like particle is not pathogenic to mice and other animals, and the safety evaluation is easy.

The invention provides a bacterium with polyethylene plastic degradation activity and application thereof, and belongs to the technical field of polyethylene plastic degradation. The bacterium with the activity of degrading the polyethylene plastic, provided by the invention, comprises at least one of pseudomonas putida ZSA123 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.34958 and enterobacter hormaechei LBA140 with the preservation number of CGMCC No.34957. The bacterium provided by the invention can obviously reduce the load, tensile stress and fracture displacement rate of a polyethylene plastic film, and when the bacterium is used for degrading polyethylene plastic, a relatively high weight loss rate can be achieved while the degradation time is shortened, and the degradation rate of the bacterium on the polyethylene plastic is far higher than that of an existing strain in the field.

According to the pogostemon cablin bacterial wilt original strain and the application thereof, enterobacter adami M42 is separated from the stem base part of a pogostemon cablin plant suffering from bacterial wilt, and the enterobacter adami M42 has pathogenicity to the plant after being inoculated with a pogostemon cablin seedling. Therefore, the enterobacter adamsii M42 can be used as a standard strain for pogostemon cablin variety disease resistance evaluation, chemical and biological agent indoor toxicity determination, field agent prevention and control research, pathogen specificity detection method development, plant-pathogen interaction mechanism research and the like. According to the application, a plurality of reagents with excellent inhibition effects are screened out through an inhibition test on enterobacter adamsii M42 and are used as the pogostemon cablin bacterial wilt prevention and treatment medicament.

The invention discloses a construction method of a crisprcas9 visual model based on fluorescent Escherichia coli, a red fluorescent protein gene is introduced into an Escherichia coli BL21 genome to construct a mcherry Escherichia coli model, and an easy-to-use biological model capable of verifying a Crisper / cas9 knockout effect and fluorescent Escherichia coli which can be quantified, is easy to observe, is high in sensitivity and is low in background are obtained.

The invention discloses a preparation method and application of a low-temperature-resistant growth-promoting complex microbial inoculant, and relates to the technical field of microorganisms. The method comprises the following steps: mixing a bacterial solution of Enterobacter sp. DNCS-S1 (P1), a bacterial solution of Enterobacter sp. DNCS-S2 (P2) and a bacterial solution of Pseudomonas sp. DNLK-S1 (K5), and inoculating the mixture into an LB liquid culture medium with the volume fraction of the inoculation amount being 1%; shaking and culturing at 4-6 DEG C for 3-4 days to obtain the low-temperature-resistant growth-promoting complex microbial inoculant. The low-temperature-resistant growth-promoting complex microbial inoculant is applied to plant growth promotion under low-temperature stress. The invention can obtain the preparation method and the application of the low-temperature-resistant growth-promoting complex microbial inoculant.

The invention provides a gene recombination escherichia coli group for synthesizing D-mannitol by catalyzing glucose through a multi-enzyme system. The gene recombination escherichia coli group comprises escherichia coli a containing insertion or enhanced expression of a glucose isomerase M2E coding gene; the escherichia coli b comprises insertion or enhanced expression of the coding gene of the NAD (H) dependent mannose-1-dehydrogenase MDH1; and Escherichia coli c comprising insertion or enhanced expression of an NAD (H)-dependent formate dehydrogenase FDH encoding gene. According to the strain and the synthesis method, glucose is used as a raw material, through multienzyme catalysis, the system comprises NADH circulation, an efficient synthesis system of D-mannitol is established, no by-product is generated in the fermentation process, high-concentration cofactors do not need to be added, and industrial production of D-mannitol is facilitated.

The application discloses a kind of plant salt stress tolerance improving rhizosphere growth promoting bacteria composition and application thereof, belong to microbial application technical field.The rhizosphere growth promoting bacteria composition of the application includes Bacillus subtilis BK73 with accession number CGMCC NO.32725 and Enterobacter ludwigii FZ20 with accession number CGMCC NO.32550.Bacillus subtilis BK73 has the activity of producing ACC deaminase, can synthesize gamma-polyglutamic acid, and Enterobacter ludwigii FZ20 has the ability of nitrogen fixation, phosphorus dissolution and IAA production.Plant experiments show that the rhizosphere growth promoting bacteria composition provided by the application exerts the synergistic effect between strains, and has obvious promoting effect on the germination of corn seeds under salt stress, the growth of corn plants and the salt stress tolerance of corn plants.The application provides a new growth promoting bacteria composition for improving plant salt tolerance, and has wide application prospect.

The present invention relates to Cap 38～228 The technical field of protein preparation, in particular, relates to a method for preparing high-purity porcine circovirus type 4 Cap protein. Steps include S1. constructing a prokaryotic expression vector pET-28a-His-SUMO-Cap with a double tag based on the nucleotide sequence of PCV4 Cap protein and His-SUMO tag. 38～228 ; S2. using pET‑28a‑His‑SUMO‑Cap 38～228 Transformed into E. coli competent cells BL21, induced expression with IPTG, and optimized expression conditions to produce the target protein His-SUMO-Cap 38～228 ; At the same time, the target protein His-SUMO-Cap 38～228 Purification; S3.His-SUMO-Cap 38～228 After SUMO protease cleavage, the His-SUMO tag was removed again using a Ni-NTA affinity chromatography column to obtain Cap 38～228 The present invention can effectively prepare high-purity PCV4Cap 38～228 The protein has a purity greater than 95%, providing PCV4Cap for serological diagnosis, polyclonal or monoclonal antibodies to Cap protein, and subunit vaccine development. 38～228 Technical basis for protein preparation.

This invention relates to a quantitative method for ultrasonic disruption of *E. coli* cells, specifically a method based on firefly luciferase in *E. coli* cells. The method involves mixing *E. coli* expressing firefly luciferase as an internal standard with a suspension of target protein-expressing bacteria, followed by ultrasonic disruption of the cells. The degree of disruption of the target protein-expressing bacterial suspension is quantitatively calculated by measuring the activity of the firefly luciferase. The target protein-expressing bacterial suspension uses *E. coli* expressing the target protein. Compared with existing technologies, this invention overcomes the shortcomings of complex experiments, low efficiency, and unstable accuracy in existing technologies. It achieves efficient and highly accurate quantification of the degree of ultrasonic disruption of *E. coli* cells, reduces experimental complexity, and improves research and development efficiency and accuracy. This provides a scientific basis and new approach for the development of new feed protein resources and livestock breeding research, and has broad application potential.

Disclosed is a broad-spectrum phage for efficiently lysing Cronobacter, a bactericide, and use thereof. Also provided is a Cronobacter phage vB_CsaM_CBT2, where the phage has a deposit number of CCTCC NO: M 2023524. The phage only specifically lyses Cronobacter, has a broad lysis spectrum, and can cover four species of Cronobacter (including multi-drug-resistant bacteria). The phage shows a desirable stability at a pH value of 3 to 11 and 25° C. to 70° C., and has a bactericidal effect of 80.55% to 99.97% within 12 h in a milk powder sample. Moreover, the phage does not carry any virulence and antibiotic resistance genes, and meets the safety requirements in practical applications. Therefore, the phage provides a new strategy and resource guarantee for the control of Cronobacter contamination in powdered infant formula (PIF) and its industrial chain and environment, as well as the development of bactericides.

The invention belongs to the technical field of microorganisms, and relates to a streptococcus salivarius subsp. Thermophilus Ss-PG-10 strain for relieving hyperuricemia and a preparation method of the streptococcus salivarius subsp. Thermophilus Ss-PG-10 strain. The strain provided by the invention is streptococcus salivarius subsp. Thermophilus Ss-PG-10, the classification name of the strain is Streptococcus salivarius subsp. Thermophilus, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. 31867. The streptococcus salivarius subsp. Thermophilus Ss-PG-10 is separated from pickled Chinese cabbages, has a function of relieving hyperuricemia, has a certain antibacterial effect on pathogenic bacteria such as staphylococcus aureus, bacillus subtilis and escherichia coli, and has relatively strong acid resistance, cholate resistance and artificial simulated gastrointestinal fluid resistance.

The present invention provides a preparation method and renaturation method of bovine lactoferrin peptide inclusion bodies, which belong to the field of biotechnology. The preparation method includes: designing the coding DNA of bovine lactoferrin peptide Bl fFf 344 based on the preferred codon of Escherichia coli, then constructing an Escherichia coli inducible expression vector and transforming it into Escherichia coli to obtain genetically engineered recombinant expression Escherichia coli; inducing the genetically engineered recombinant expression Escherichia coli to efficiently express the bovine lactoferrin peptide Bl fFf344 and form inclusion bodies with isopropylthiogalactoside to obtain a bacterial mixture; centrifuging the bacterial mixture to collect the Escherichia coli cells, resuspending them and performing a cell wall breaking process, and then centrifuging to collect the bovine lactoferrin peptide inclusion bodies. The method can efficiently prepare bovine lactoferrin peptide inclusion bodies, and combined with the bovine lactoferrin peptide inclusion body separation, dissolution, purification and renaturation method established by the present invention, realizes genetic engineering and efficient production of bovine lactoferrin peptide.

This invention belongs to the field of biosensing and fiber optic detection technology, and discloses a photoelectric-coupled fiber optic real-time in-situ detection system for monitoring the adsorption process of *E. coli* (DH5α). Addressing the core pain points of existing *E. coli* detection technologies, such as reliance on complex sample pretreatment (taking over 24 hours) and the inadequacy of false positives and sensitivity with single-fiber SPR or electrochemical detection, which fail to capture the dynamic process of bacterial adsorption, this invention uses a fiber optic SPR probe with a dual recognition layer consisting of a surface-modified dopamine polymer membrane, an LPS-specific nucleic acid aptamer, and a *E. coli* shape-imprinted cavity as the working electrode. It simultaneously acquires SPR resonance wavelength shift and electrochemical current signals, achieving cross-validation of multi-dimensional detection results. The system can be in-situ implanted into complex detection systems such as water samples and food homogenates without labeling. It exhibits strong anti-interference capabilities and high detection specificity, and can track the entire dynamic process of *E. coli* adsorption-desorption in real time. It can be widely applied in scenarios such as rapid on-site screening of foodborne pathogens, research on bacterial interfacial adsorption mechanisms, and monitoring of microbial pollution in aquatic environments.

This invention relates to the field of genetic engineering technology, and particularly to TEV protease mutants, their preparation methods, and applications. This invention modifies the TEV enzyme molecule, with mutation points differing from those reported in previous studies. This enhances the activity of the TEV protease at the molecular level. Furthermore, this invention utilizes CRISPR / Cas9 gene editing techniques to modify the host bacterial chassis, obtaining engineered strains that express TEV at high yields. Further, the provided high-density fermentation control method can rapidly promote TEV enzyme expression, resulting in high E. coli cell yield, with an OD600 value reaching approximately 150, a cell wet weight of 80–130 g / L, and high expression levels of the target protein, accounting for 40%–64% of the total bacterial protein. Simultaneously, the method provided by this invention is simple and easy to implement, using readily available raw materials, achieving good application results while reducing production costs, resulting in significant economic benefits.

The invention discloses a construction method and application of an engineering bacterium for increasing the yield of alpha-ketoglutaric acid. Wild E.coli.W3110 serves as a chassis strain, lactic dehydrogenase ldhA, pyruvate dehydrogenase poxB and formic acid C-acetyltransferase pflB are knocked out firstly, and the flow direction of pyruvic acid to lactic acid, acetic acid and formic acid is blocked; meanwhile, the promoter PBBaJ23109 is used for replacing a natural promoter of the sucA gene, so that alpha-ketoglutaric acid dehydrogenase is weakened, and the decomposition way of alpha-ketoglutaric acid is weakened; the phosphoenolpyruvate carboxylase ppc is overexpressed, and the flow direction of phosphoenolpyruvate to oxaloacetic acid is enhanced; isocitrate dehydrogenase icd from escherichia coli is overexpressed, so that synthesis of a product is promoted; besides, pyruvate dehydrogenase derived from saccharomyces cerevisiae is introduced and coded by genes PDB1, PDA1 and LAT1, so that an important precursor, namely pyruvic acid, is promoted to flow to acetyl coenzyme A; the engineering strain with high yield of alpha-ketoglutaric acid is finally obtained, the yield of 75.1 g / L can be achieved in a 5L fermentation tank through a fed-batch fermentation method for 40 hours, and the conversion rate is 39.6%.

An E. coli bacterial diagnostic device comprises an outer body with a first chamber for fluid intake and a second chamber for a lateral flow test strip holder, featuring a results viewing window. An inner body, rotatable within the first chamber, includes a fluid receiving chamber and an opening for fluid passage. A filter located between the outer and inner bodies ensures fluid filtration. A lateral test strip holder, located in the second chamber, can move between a test position in which a portion of a lateral flow test strip located in the lateral test strip holder is positioned in the first chamber adjacent the filter and a second retracted position in which the lateral flow test strip is positioned in the second chamber.

The invention provides an enterobacter bacterium Ct2, a microbial agent, a microbial fertilizer and application of the enterobacter bacterium Ct2, and belongs to the technical field of agricultural microorganisms. The invention provides an enterobacter bacterium Ct2, and the preservation number of the enterobacter bacterium Ct2 is CCTCC (China Center for Type Culture Collection) NO: M 2025569. The enterobacter sp. Ct2 is separated from rhizosphere soil of safflower and has the effects of secreting IAA, efficiently dissolving phosphorus and reducing the pH value. The enterobacter sp. Ct2 can effectively promote the growth and development of safflower with different flower colors, increase the yield of safflower fruit balls, increase the content accumulation of active components in filaments of safflower with different flower colors in different flowering days, and improve the physicochemical properties of safflower rhizosphere soil at the same time. In conclusion, the enterobacter sp. Ct2 provided by the invention can be applied to preparation of microbial fertilizers and microbial agents, and lays a foundation for research and development of special, efficient and green microbial fertilizers.

The invention provides a blood microorganism-based autism prediction model and application thereof, the prediction model takes blood microorganisms as markers, and the detection rate or relative abundance of the blood microorganisms is reduced; the risk that the blood sample suffers from autism is predicted by increasing the detection rate of blood microorganisms II or enhancing the relative abundance; the first blood microorganisms comprise ochrobactrum, pseudoalteromonas sp.3J6, rhodococcus equi, parabacteroides dieldrii, trichoderma harzianum, enterobacter hormaechei, wolbachia, yersinia enterocolitica, treponema pallidum and Elizabeth mosquito-repellent bacteria; the blood microorganisms II comprise Klebsiella compacticola and bdellovibrio bacteriovorus. According to the method, the model is constructed based on the blood metagenome data of 1946 standard autism four families, the advantages of blood microorganisms are brought into full play, disease signals are sensitively and accurately captured, the method can be applied to clinical autism prediction, and early risk assessment and layering of diseases are achieved.