129 results about "Enterobacter sp" patented technology

The invention discloses a plant lactobacillus with a bacteriostatic effect and application of the plant lactobacillus. The lactobacillus plantarum is named as lactobacillus plantarum C07, the preservation number is CCTCC NO: M20242086, the preservation unit is China Center for Type Culture Collection, the preservation time is September 26, 2024, and the preservation address is Wuhan University, Wuhan University, No. 299, eight road, Wuchang District, Wuhan City, Hubei Province. The strain can produce lactic acid and indole-3-lactic acid at high yield, has a very strong antibacterial effect on multiple putrefying and pathogenic microorganisms such as staphylococcus aureus, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella and mould, and is a broad-spectrum antibacterial microorganism.

The invention provides a method for constitutive expression of foreign protein by escherichia coli Nissle 1917, and belongs to the technical field of genetically engineered bacteria. The invention provides a method for constitutive expression of foreign protein by Escherichia coli Nissle 1917 (EcN), which is characterized in that EcN is used as a chassis strain, an engineering strain for constitutive expression of the foreign protein is constructed by transforming a covert plasmid, and high-efficiency expression of the foreign protein can be realized without induction of an inducer and antibiotic pressure. By utilizing the method disclosed by the invention, the EcN engineering bacterium for constitutive expression of the foreign protein RtSOD is successfully constructed, constitutive efficient expression of the high-stability RtSOD protein in the EcN engineering bacterium can be realized without using antibiotic pressure and inducer induction, and the EcN engineering bacterium can be used as a potential viable bacterium medicine for preventing and treating oxidative stress related diseases and has a wide application prospect. And the method has important significance on the practical application of the EcN engineering strain.

The invention relates to the field of environment, phosphorite resource utilization and agricultural biotechnology, in particular to a phosphate ore-containing phosphate-solubilizing bacterial agent, a preparation method thereof, a biological bacterial fertilizer and application, the phosphate ore-containing phosphate-solubilizing bacterial agent comprises phosphate-solubilizing bacteria, hydroxyapatite and a culture medium, and the phosphate-solubilizing bacteria are enterobacter hormaechei HS-6. The hydroxyapatite is used for preparing the phosphate ore-containing phosphate-solubilizing bacterial agent, so that the phosphate ore resource can be greatly promoted to be fully utilized, and the dissolution of insoluble phosphorus in the phosphate ore is realized. According to the phosphate-containing ore phosphate-solubilizing bacterial agent, the enterobacter hormaechei HS-6 is combined with the hydroxyapatite, so that the phosphate-solubilizing capacity of the enterobacter hormaechei HS-6 can be utilized, and the phosphorus resource of the hydroxyapatite can be fully utilized. The phosphate-containing ore phosphate-solubilizing bacterial agent has good prospects in application to preparation of biological bacterial fertilizer, application to agricultural soil improvement, application to phosphate ore resource recovery and application to promotion of wheat growth.

The invention discloses application of polypeptide compounds in prevention and treatment of pathogenic microorganisms. The compound has a synergistic effect on agricultural fungi such as rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium graminearum, Pyricaria oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae and pyricularia oryzae. The method comprises the following steps: carrying out sterilization on plant bacteria such as Xanthomonas oryzae PXO99A, Xanthomonas axonophora dispsp.citrijx-6, Pseudomonas solanacearum (Smith) BNCC335855, Pseudomonas sp. The method is characterized by comprising the following steps of: killing Aspergillus flavus ACCC32601 (Aspergillus flavus), Penicillium expansum ACCC 30898 (Penicillium expansum. EXPANSum ACCC 30898), Monilinia coerulea ACCC 36263 (Aspergillus oryzae, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger and Aspergillus niger); the compound disclosed by the invention has a certain inhibition activity on various diseases caused by streptococcus agalactiae ATCC 27956, streptococcus dysgalactiae ATCC27957, Escherichia coli ATCC 25922 and Staphylococcus aureus, particularly, the compound Thiostreptin has a particularly remarkable inhibition effect on agricultural pathogenic bacteria, food-borne germs and cow mastitis germs, and is expected to be developed into a novel antibacterial drug, so that the compound disclosed by the invention can be used for preparing the antibacterial agent for preventing and treating various diseases caused by the streptococcus agalactiae ATCC 27956, the streptococcus dysgalactiae ATCC27957, the Escherichia coli ATCC 25922 and the Staphylococcus aureus.

The invention discloses a method for improving the solubility and thermal stability of sweet protein, and belongs to the technical field of biosynthesis, the method comprises the following steps: constructing a gene tandem recombinant plasmid containing a monellin x-3C-sfGFP-3C-monellin y expression cassette, x is greater than or equal to 1, y is greater than or equal to 1, 5 is greater than or equal to x + y is greater than or equal to 3, a 3C protease recognition sequence is also inserted between adjacent copies of monellin, and each monellin is connected with a purification tag; the gene tandem recombinant plasmid is transformed into escherichia coli and inducible expression is carried out, thalli are split and purified to obtain fusion protein, 3C protease is used for enzyme digestion, and the target protein is obtained after re-purification. The total protein yield and the solubility proportion are remarkably improved by adopting a series construction mode of plasmids, meanwhile, the stability of monellin is effectively improved, the purification step is simpler and more convenient, and the method is suitable for industrial production.

The invention discloses enterobacter hormaechei and an application of the enterobacter hormaechei in preparation of a heavy metal removing agent. The enterobacter hormaechei FGS-CS4 is preserved in Guangdong Microbial Culture Collection Center on June 15, 2023, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 63564. The copper adsorption rate of the strain can reach 75.13% in 24 h, and the lead adsorption rate of the strain can reach 99.57%. The enterobacter hormaechei FGS-CS4 disclosed by the invention is high in tolerance and adsorption rate to copper and lead, can be used for resisting stress of heavy metals, and has a wide application prospect in adsorption and removal of copper and lead.

The invention relates to a strain for degrading polyacrylamide and application thereof in the technical field of destabilization treatment of oilfield produced water, the strain for degrading polyacrylamide is separated and screened from Jiangsu oilfield lamellar bank produced water, specifically enterobacter HY1, is preserved in China General Microbiological Culture Collection Center, and has the preservation number of CGMCC (China General Microbiological Culture Collection Center). The enterobacter sp.HY1HY1 strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO 32105, the preservation time is October 9, 2024, the enterobacter is classified and named as enterobacter, the Latin literature is named as Enterobacter sp.HY1HY1, and the enterobacter sp.HY1HY1 strain has a gene sequence shown as SEQ ID NO: 1. The enterobacter HY1 has the characteristics of medium and high temperature resistance and hypersalinity resistance, the strain can effectively degrade polyacrylamide in shale oil produced water under the condition, and effective treatment of polyacrylamide in shale oil produced water in an oil field is realized.

The present disclosure provides a recombinant Escherichia coli strain modified by metabolic engineering means and a method for producing L-homoserine by using the same. The strain, designated as Escherichia coli having a strain number of 13-XA, is deposited in China General Microbiological Culture Collection Center (CGMCC) with an accession number of CGMCC No. 25099, dated Jun. 16, 2022. With respect to the chromosome DNA thereof, one or more genes associated with fatty acid metabolism are knocked out or attenuated, and / or a promoter is replaced for enhancement; one or more genes associated with the L-homoserine metabolic pathway are knocked out or attenuated, and / or one or more genes associated with the L-homoserine metabolic pathway are overexpressed or enhanced, and / or one or more genes associated with the L-homoserine metabolic pathway are mutated.

The invention provides a complex microbial inoculant for producing beta-glucuronidase and application of the complex microbial inoculant in calculus bovis production, and relates to the technical field of microorganisms. The culture method of the complex microbial inoculant provided by the invention comprises the following steps: culturing lactobacillus ATCC 53103 in an initial culture medium to a logarithmic phase, sterilizing to obtain a complex culture medium, mixing the complex culture medium with nutritional ingredients, inoculating escherichia coli ATCC 25922 and riemerella CCUG 14215, and co-culturing to obtain the complex microbial inoculant. The complex microbial inoculant provided by the invention can generate a large amount of high-activity beta-glucuronidase, so that the efficiency of in-vitro production of calculus bovis can be effectively improved.

The invention relates to the technical field of functional microorganism screening and application, in particular to a composting agent capable of composting at low temperature, normal temperature and medium-high temperature as well as a preparation method and application thereof. The decomposition agent comprises an active component and an adsorption carrier; wherein the active ingredients are lysine bacillus, klebsiella and enterobacter; the rice hull biochar is used as a carrier, and the obtained compound microbial agent is a powder microbial agent and can be used for composting and aeration in different temperature environments such as low temperature, normal temperature and medium-high temperature. By screening and optimizing strains and combining with reasonable formula design, the activity of the decomposition agent in low-temperature, normal-temperature and medium-high-temperature environments is remarkably improved, the technical problems of narrow applicable temperature range, low efficiency, poor product quality, potential environmental risk and the like of the decomposition agent in the prior art are solved, annual decomposition can be realized, and the decomposition efficiency is improved. The method is not affected by seasons, resources are fully utilized, and the resource utilization efficiency is improved.

The present invention discloses an antimicrobial peptide and its application, wherein the antimicrobial peptide comprises one or more of an antimicrobial peptide PFK having an amino acid sequence as shown in SEQ ID NO: 1, an antimicrobial peptide PVK having an amino acid sequence as shown in SEQ ID NO: 2, and an antimicrobial peptide PIK having an amino acid sequence as shown in SEQ ID NO: 3. The antimicrobial peptides PFK, PVK and PIK of the present invention have antimicrobial effects on Porphyromonas gingivalis, Streptococcus mutans, Acinetobacter baumannii, Escherichia coli and Staphylococcus aureus, and the antimicrobial peptides PFK, PVK and PIK have low hemolytic activity and low cytotoxicity, and can be used to prepare biological antimicrobial preparations, anti-infective drugs or oral products.

The invention discloses lactobacillus arrhizatae and application thereof, and relates to the technical field of microorganisms. The lactobacillus arrhizatae is preserved in the Guangdong Microbial Culture Collection Center on December 10, 2024, the preservation number is GDMCC 65603, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. According to the invention, a strain of Lactobacillus argenteus is screened from healthy free-ranging adult local partridge chickens from Guangxi Zhuang Autonomous Region Tibetan City, the strain is named as LB19, and verification proves that the Lactobacillus argenteus has a good inhibition effect on food-borne pathogenic bacteria including salmonella and escherichia coli, and can be used for preparing food-borne pathogenic bacteria. Good application potential is realized in the aspect of preventing and treating food-borne pathogenic bacteria.

The invention belongs to the field of escherichia coli metabolic processes, and particularly relates to a method for producing D-psicose from escherichia coli through glucose fermentation. Escherichia coli is used as a chassis host bacterium, a pgi gene, an SUMO-alsE fusion gene and an a6PP gene are subjected to overexpression at the same time, then a pfkA gene, a pfkB gene and a rpiA gene are knocked out in sequence, and a recombinant bacterium capable of producing D-psicose through glucose fermentation is obtained; the recombinant bacteria are used for fermentation, and glucose is converted to generate D-psicose.

The invention relates to a recombinant engineering bacterium for expressing pure iron nitrogenase as well as a construction method and application of the recombinant engineering bacterium, and belongs to the field of biotechnology and bioengineering. Key gene clusters of rhodopseudomonas and Vilnelande nitrogen-fixing bacteria are amplified and cloned into an expression vector, escherichia coli is converted to obtain recombinant engineering bacteria for expressing pure iron nitrogenase, and the pure iron nitrogenase expressed by the recombinant engineering bacteria can reduce carbon dioxide (CO2) into methane (CH4); the recombinant engineering bacteria are cultured in an LB culture medium added with ferric chloride, ampicillin and spectinomycin, oxygen exposure is reduced as much as possible under the optimized semi-anaerobic condition, the enzyme activity can be improved to the maximum extent, and compared with a traditional system, the methane yield is remarkably increased; by providing a novel methane production scheme, the technical defects of low efficiency and oxygen sensitivity of the traditional nitrogenase process are improved; the process of the present invention contributes to carbon capture and utilization by converting waste carbon dioxide into valuable methane.

The invention provides a method for producing beta-glucuronidase from escherichia coli and application of the beta-glucuronidase, and relates to the technical field of microorganisms. The method provided by the invention comprises the following steps: inoculating Escherichia coli ATCC 25922 on a composite culture medium for pre-culture, and culturing in an electric field environment of 1-10N / C for 12-24h to obtain a mixture containing beta-glucuronidase; wherein the composite culture medium comprises 10-100 [mu] mol / L of ferric citrate, and the concentration of the ferric citrate is increased in stages in the pre-culture process. According to the method, the escherichia coli ATCC 25922 is cultured in the iron-containing culture medium and the low-intensity electric field environment, so that the synthesis of the beta-glucuronidase by the escherichia coli ATCC 25922 can be effectively improved, and the enzyme activity and the enzyme yield of the beta-glucuronidase are improved.

The invention discloses a conservative B cell epitope peptide of goose parvovirus VP3 protein, a nucleic acid molecule, a recombinant vector and application thereof. Conservative B cell epitope EW12 is inserted into F18 Escherichia coli Fod fimbriae, inert carrier bacteria S9H are introduced, recombinant bacteria capable of functionally exhibiting and expressing the EW12 conservative B cell epitope on the surface of the bacteria are obtained, the recombinant bacteria can specifically generate specific binding reaction with gosling plague egg yolk antibody and gosling plague vaccine immune serum, and the gosling plague egg yolk antibody and the gosling plague vaccine immune serum can be specifically combined with the gosling plague egg yolk antibody and the gosling plague vaccine immune serum. The method has the advantages that macroscopic agglutination particles are generated, cross agglutination reaction with other pathogen positive serum and healthy goose serum is avoided, the method is good in sensitivity, and the specific antibody can be detected in the 5th day after immunization at the earliest. A plate agglutination test directly mediated by the goose parvovirus VP3 protein conservative B cell epitope peptide EW12 has the advantages of specificity, sensitivity, convenience in operation and suitability for on-site large-scale rapid detection, and is expected to provide a new technical means for diagnosis, prevention and control and purification of GPV infection.

The invention relates to a microbial agent for degrading COD (Chemical Oxygen Demand) in DBP (Dibutyl Phthalate) wastewater as well as a preparation process and application of the microbial agent. The microbial agent is prepared from the following active components in parts by weight: 88 to 91 parts of pseudomonas, 5.2 to 5.6 parts of enterobacter and 0.05 to 0.11 part of alcaligenes. According to the application, a plurality of microbial strains are formed in the microbial liquid which is subjected to mixed domestication culture of the DBP wastewater, the anaerobic sludge, the hydrolytic acidification bacteria and the nutritive salt, and a plurality of microbial strains in the microbial agent which is obtained by mixing the microbial liquid after bacteria screening and expanding culture have a synergistic effect, so that the microbial agent has the performance of efficiently degrading COD in the DBP wastewater in an environment-friendly manner.

The subject relates to an isolated antibody that specifically binds to O25b antigen of multi drug resistant (MDR) E. coli strains, its medical and diagnostic use, method of producing the antibody, including an isolated nucleotide sequence, plasmids and host cells as used in the production of the antibody; and further an isolated epitope recognized the specific antibody.

The invention relates to the technical field of microorganisms and compositions thereof, and particularly discloses a preparation method and application of a saline-alkaline tolerant complex microbial inoculant. The saline-alkaline tolerant complex microbial inoculant is prepared from the following raw materials in parts by weight: 15-20 parts of an arbuscular mycorrhizal fungal spore mixture; 20 to 25 parts of a growth-promoting bacterium solution; 60-70 parts of a matrix; 4-5 parts of a nutrient substance; a strain in the growth-promoting bacterium liquid comprises one or more of enterobacter luviveilis, serratia marcescens and alcaligenes faecalis, and the matrix comprises common biochar and diatomite, and the microelement comprises one or more of the enterobacter luviveilis, the serratia marcescens and the alcaligenes faecalis. The preparation method comprises the following steps: S1, preparing a bacterial liquid; s2, carrier preparation; and S3, mixed fermentation. The product can be used for improving saline-alkali soil, the application amount is 2-4 kg / hm < 2 >, the product has the advantages of being high in survival rate and activity, and the organic matter content of soil and the survival rate of plants in saline-alkali soil can be effectively increased.

The invention discloses a multifunctional composite flora capable of degrading atrazine at low temperature and increasing phosphorus as well as a preparation method and application of the multifunctional composite flora, and relates to the technical field of farmland soil pollution microbial remediation. The invention aims to solve the problem of poor atrazine pollution remediation effect of a single strain in a low-temperature environment. The preparation method comprises the following steps: mixing a simple pure bacillus C1 bacterial solution, an enterobacter (Enterobacter sp.) P1 bacterial solution and an Acinetobacter lwoffii DNS32 bacterial solution, so as to obtain the multifunctional composite flora capable of degrading the atrazine at the low temperature and increasing the phosphorus, which is disclosed by the invention, and the preparation method comprises the following steps: mixing the bacterial solution of the simple pure bacillus, the bacterial solution of the enterobacter (Enterobacter sp.) P1 bacterial solution and the bacterial solution of the Acinetobacter lwoffii DNS32 bacterial solution, so as to obtain the multifunctional composite flora capable of degrading the atrazine at the low temperature and increasing the phosphorus. The invention can obtain the multifunctional composite flora capable of degrading atrazine at low temperature and increasing phosphorus as well as the preparation method and the application of the multifunctional composite flora.

Primers for of amplifying DNA from shiga toxin producing E. coli (STEC); methods of amplifying STEC DNA; and methods of detecting STEC.

Antimicrobial eyewear features frames infused with or coated with antimicrobial additives, thereby offering continuous protection by actively destroying and inhibiting the growth of bacteria, fungi, parasites, and some viruses. The lenses may also have an antibacterial coating that destroys and inhibits bacterial growth. In some examples, the lenses are treated with a specialized strengthening liquid, wherein the liquid contains nano components that release heavy metal ions capable of invading the cell walls of bacteria, leading to bacterial elimination, destroying DNA molecules and proteases within the bacterial cells, denature viral proteins, break DNA chains, and result in cell death. Additionally, the strengthening liquid reduces dehydrogenase activity and interacts with various protein groups within the cell, thereby reducing the activity of these groups and effectively inhibiting the growth of E. coli and other bacteria on the lens surface.

The application discloses a soil enterobacter capable of efficiently degrading polypropylene and application thereof. The strain is FJNUIM-S2, which is classified and named as soil enterobacter (Enterobacter agglomerans) Enterobacter soli ), is preserved in the China General Microbiological Culture Collection Center (CGMCC), and is preserved on October 17, 2024, with a preservation number of CGMCC No. 32241 and a preservation address of No. 3, Institute of Microbiology, Chinese Academy of Sciences, Xiliujiaobei, Chaoyang District, Beijing. The strain FJNUIM-S2 of the application can grow with polypropylene as the only carbon source, has good polypropylene degradation characteristics, provides a microbial resource for bioremediation of waste polypropylene in the environment, and has a wide application prospect.

Recombinant E. coli strains and synthetic protein sequence designs are leveraged for production of disordered polypeptides such as spidroins and elastin-like peptides (ELPs). These disordered polypeptides, the high-titer production of which has proven difficult, include repeating structural motifs from a small selection of amino acid residues, resulting in lack of well-defined tertiary and quaternary structure. The recombinant E. coli include expression vectors with genes encoding for the disordered polypeptide product. Expression of these genes is controlled by a promoter that downregulates and substantially inhibits basal expression in the recombinant bacteria. Further, the recombinant bacteria include mutations to one or more stress-response genes from wild-type E. coli, such as yggw, yedv, yedw, yedy, spec, speb, uspc, hcha, loip, mltc, envz, ompr, yhgf, or hupb. The recombinant E. coli enable production of high titers of disordered protein product while minimizing the toxic effects thereof on the host.

The application belongs to the field of enzyme engineering and biotechnology, and discloses a 4-hydroxybenzoic acid hydroxylase mutant and application thereof in synthesis of gallic acid or a salt thereof. The 4-hydroxybenzoic acid hydroxylase mutant is obtained through directional evolution modification and high-throughput screening, and has an increased protocatechuate hydroxylase activity by more than 4 times. The 4-hydroxybenzoic acid hydroxylase mutant is expressed by using E. coli, and the obtained recombinant E. coli can efficiently catalyze the conversion of protocatechuate into gallic acid. The 4-hydroxybenzoic acid hydroxylase mutant is expressed in the recombinant E. coli for producing protocatechuate, and the obtained recombinant E. coli can de novo synthesize gallic acid from glucose as a substrate, and the yield reaches 58.65 g / L, and the residual amount of protocatechuate is only 0.33 g / L. The application solves the problem of a large amount of residual protocatechuate in the fermentation process of the existing strains, and can provide an efficient enzyme element for the creation of gallic acid industrial strains.

The invention provides an escherichia coli engineering bacterium for high yield of yellow cochineic acid and a construction method of the escherichia coli engineering bacterium, and a modified chassis cell escherichia coli BAP1-pflB is obtained by performing gene knockout on a pflB gene in an escherichia coli thallus BAP1. And the constructed recombinant plasmid pET30a-antDEFBG + zhuIJ is electrically transformed into escherichia coli BAP1-pflB, so that the engineering bacterium BAP1-pflB-PFK capable of producing the yellow cochineic acid is obtained. Through HPLC (High Performance Liquid Chromatography) detection, after the gene pflB is knocked out, the yield of the yellow cochineic acid is obviously increased. Yellow carmine acid synthesized by the escherichia coli engineering bacteria is a high-grade precursor of carmine acid with high commercial value, and plays an important role in the fields of textiles, skin care products and the like.

The invention belongs to the technical field of microbial treatment, and particularly relates to enterobacter hormaechei and application thereof.The enterobacter hormaechei has the function of efficiently dissolving out high-valence metal cations such as Fe < 2 + > / 3 < + >, Ca < 2 + > and Mg < 2 + > from iron tailings, the functional strain is used for treating coastal solonchak, dissolved-out free-valence metal ions can accurately target Na < + > adsorbed by soil colloid, and by means of the ion exchange effect, the soil colloid can be accurately adsorbed by the dissolved free-valence metal ions; na < + > is desorbed from a saline soil body and discharged out of plough layer soil, the problems that the coastal saline soil is high in salinity and single in component can be effectively solved, and an advanced and reliable technical means is provided for ecological restoration and agricultural utilization of the coastal saline soil.

The invention relates to the technical field of medicines, in particular to skin antibacterial transdermal liquid and a preparation method thereof. The traditional Chinese medicine composition is prepared by mixing the following raw materials in percentage by weight: 0.1%-0.4% of radix euphorbiae lantu, 0.25%-0.75% of rhizoma smilacis glabrae, 0.15%-0.55% of honeysuckle flower, 0.08%-0.38% of radix salviae miltiorrhizae, 0.2%-0.8% of flos chrysanthemi indici, 0.05%-0.55% of spina gleditsiae, 0.05%-0.45% of typhonium giganteum, 3%-8% of borneol, 0.25%-0.6% of radix sophorae flavescentis, 0.2%-0.65% of fructus cnidii, 0.15%-0.45% of fructus kochiae, 0.01%-0.45% of radix stemonae and 0.02%-0.55% of chlorhexidine acetate. The skin antibacterial transdermal liquid has the beneficial effects that through the synergistic interaction of various natural traditional Chinese medicine components and the combination of a modern extraction process, effective components are fully reserved, the skin antibacterial transdermal liquid has a remarkable inhibition effect on various skin pathogenic bacteria such as staphylococcus aureus, escherichia coli and candida albicans, and the antibacterial rate can reach 90% or above; meanwhile, the traditional Chinese medicine is used for improving skin microcirculation, and penetration promoting components such as borneol are used, so that the medicine can quickly penetrate through the skin cuticle and penetrate into the skin tissue to exert the medicine effect, the symptoms such as skin itch and inflammation are effectively relieved, the preparation process is relatively simple, and industrial production is easy.

The present invention relates to a recombinant microbial system for generation of neo-glycocins, a substrate and to co-evolve its glycosyltransferase enzyme. The recombinant microbial system comprises a gene cassette A encoding for microbial O- and S-glycosyltransferase and its suitable acceptor substrate in conjugation with a cleavable dual affinity tag under the control of two independent inducible promoters. The gene cassette is expressed in a microbial host such as E. coli for the co-expression of glycosyltransferase and its suitable acceptor substrate. The invention further discloses method for production and bioactivity guided screening of O- and or S-neo-glycocins using the recombinant microbial system. The system provides optimized construct design, and methods for high yield production of glycocins and neo-glycocins for downstream applications.

The present invention belongs to the field of development and application of Chinese medicine resources, and specifically relates to a preparation method and application of a Morchella solid-state fermented geranium seedling extract with antibacterial effect. The preparation method is realized by the following steps: activating the Morchella strain by PDA solid culture medium, then inoculating it into the PDA culture medium containing geranium seedling for fermentation, extracting by 70% ethanol, centrifuging the extract, and freeze-drying after rotary evaporation to obtain a Morchella fermented geranium seedling extract (solid-state fermentation). The Morchella fermented geranium seedling extract prepared by the present invention is subjected to a broad-spectrum antibacterial experiment on pathogenic bacteria such as Staphylococcus aureus, Escherichia coli, and Bacillus aerogenes. It is found that the Morchella fermented geranium seedling extract of the present invention can inhibit the growth of Staphylococcus aureus, Escherichia coli, and Bacillus aerogenes, and has a significant antibacterial effect, which provides a scientific basis for the research on the antibacterial application of Morchella fermented geranium seedling extract.