240 results about "Enterobacter sp" patented technology

The invention relates to a mutant strain of bacteria, which either lacks or contains mutant genes for several key metabolic enzymes, and which produces high amounts of succinic acid under anaerobic conditions.

The invention provides compositions and methods for engineering E. coli or other host production bacterial strains to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.

The invention discloses a bacterial strain b capable of resisting the growth of heavy metal Cd and reducing sulfate, and a bacterial strain capable of resisting the growth of the heavy metal Cd and solubilizing phosphate. The classification and name of the bacterial strain b and the bacterial strain are respectively enterbacter ludwigii SRB-2-5U-2 and pseudochrobactrum saccharolyticum LB-4-4-1c, which are already preserved in the Common Microbe Center of the China Committee for Culture Collection of Microorganisms. The bacterial strains disclosed by the invention can be used for simultaneously remediating the surface soil, deep soil and rhizosphere soil of a plant, reducing the pollution of Cd to surface water and underground water, and also improving the activity and fertility of the soil.

The invention relates to a micro-capsulated suspended microbial seed coating agent and a preparation method thereof. The micro-capsulated suspended microbial seed coating agent comprises a functional bacteria microcapsule and auxiliaries, wherein the functional bacteria microcapsule is prepared from wall materials such as sodium alginate and gelatin by virtue of an endogenous emulsion process, the grain size of the microcapsule is 20-60 mu m, the content of functional bacteria is not lower than 10<9>cfu / g, and the embedding rate is over 80%. Functional microbes are selected from two or more of pseudomonas, bacillus subtilis, bacillus amyloliquefaciens, enterobacter cloacae, raoultella planticola and bacillus atrophaeus. The auxiliaries comprise one or more of a film-forming agent, a dispersant, a tackifier, an anti-freezing agent and a coloring agent. The prepared functional bacteria microcapsule is uniformly mixed with the auxiliaries to obtain a finished product. The product has the advantages of good film-forming performances, thallus persistence, high survivability, good slow-controlled release, strong application stability, environment friendliness, convenience in use, and the like, can be used for promoting seed germination and seedling growth of cottons, soya beans, wheat and potatoes, and the like, controlling the diseases at the seedling stage, improving stress resistance, and the like.

This invention relates to a fusion protein used for preventing aftosa, its preparation method and application. This fusion protein contains O type foot-and-mouth disease virus main cytomembrane protein VP1 epitope, colibacillus thermolability toxin B subunit, thymus derived cell epitope and purification label.

The invention belongs to biology technology field, which concretely refers to controlled release tissue factor path inhibitor (LTFPI) and the manufacturing method, and the application. The invention analyzes and experiments the biology information science and structure molecular biology science of tissue factor path inhibitor (TFPI) and its acceptor low density lipoprotein acceptor correspondent protein (LRP), it ascertains the part where the TFPI carboxy end combines with LRP and is eliminated. It designs TFPI carboxy end mutant, which is recombined with primary nucleus or eukarya expressing carrier after constructing LTFPI gene through PCR location, it converts bacillus coli or bici yeast, sifts the high expression project fungus. The primary project fungus are yeasted and expanded, crushed, then they are centrifugated and collects the inclusion body, purifies the LTFPI through molecular sift and ion interchanging two-step method; the eukarya project fungus are carried on with two-step purifying directly. The half-life are prolonged, it has good pour-depressant function.

The invention relates to a pig breeding and breathing complex virus (PPRSV) and pig 2-type loop virus (PCV 2) series gene recombination adenovirus and vaccine in the field of high and new biotechnology. It uses PCR technology to clone the Cap protein gene into the plasmid carrier pShuttle-CMV-GP5 by open type reading rule and transforms tobacillus coli BJ5183strain with cage carrier pAdEasy-1 to capture the recombination plasmid; it uses recombination plasmid HEK293-A cell to capture recombination adenovirus and purifies it to express the recombination adenovirus rAd-Cap-GP5 of PRRSV GP5protein and PCV-2 Cap protein.

Pharmaceutical compositions of probiotic E. coli strains and uses thereof for treating inflammatory bowel disease.

The invention provides various skin mucous membrane disinfectants which are prepared by adopting double-chain quaternary ammonium salt and benzalkonium bromide (C20H40BrN) as main germicidal ingredients and using triclosan (C12H7Cl3O2), polyethylene glycol (HOCH2(CH2OCH2)nCH2OH), glycerol (C3H8O3), Tween 80, menthol, borneol, medical alcohol and the like as adjuvant ingredients, can be used in thefields such as the disinfection of skin and mucosa, the disinfection of surgical hand washing and the disinfection of medical apparatus and instruments in the hospital, the disinfection of tools anddevices in public places and different production industries, the mold prevention of industrial products and agricultural grains, the health disinfection of poultry houses, the aquatic product field,the aquaculture, the killing of alga, the preparation of plastic antibacterial agent, the preparation of compound disinfectant and the like and has effective bactericidal action to staphylococcus aureus, colibacillus, candida albicans, HIV and the like ; in addition, the preparation method is simple and the production cost is lower.

The invention discloses sulfate reducing bacteria with tolerance on arsenic. The sulfate reducing bacteria are named as enterobacteria (enterobacter sp.) taihuN3 in classification, wherein the bacterial strain is preserved in General Microbiology Centre of China Committee for Culture Collection of Microorganisms; the preservation number is 23 Nov 2012; and the preservation number is CGMCC No.6886. Application of the sulfate reducing bacteria with tolerance on arsenic in wastewater treatment is also disclosed by the invention. The bacterial strain disclosed by the invention is wide in utilizable carbon source range, easy to cultivate, strong in growth metabolic activity, and high in sulphate conversion efficiency. The bacterial strain disclosed by the invention can normally grow when the concentration is 10-1000mg / LAs(V); and the percent reduction of the bacterial strain disclosed by the invention under 200mg / LAs(V) of sulfate achieves 50%. The sulfate reducing bacteria can be used for processing high-concentration arsenic wastewater, and has good application prospect.

Methods of increasing yields of succinate using aerobic culture methods and a multi-mutant E. coli strain are provided. Also provided is a mutant strain of E. coli that produces high amounts of succinic acid.

The invention provides a method for producing extracellular pullulanase by applying an auto-induction culture medium and a dual-temperature control strategy, belonging to the technical field of pullulanase production through microbial fermentation. The method has the following beneficial effects: pullulanase coding genes from Klebsiella variicola CCTCC M2012108 are inserted into an expression vector pET28a(+) to construct recombinant plasmids and E.coli is converted to obtain a recombination strain E.coli BL21(DE3) / pET28a(+)-pulA containing the target pullulanase gene; the auto-induction culture medium is utilized to culture the recombinant E.coli BL21(DE3) / pET28a(+)-pulA and ferment the recombinant E.coli BL21(DE3) / pET28a(+)-pulA to generate enzyme by adopting the dual-temperature control mode of firstly culturing at 37 DEG C for 2-4 hours and then continuing culture at 25 DEG C for 48-72 hours; and after adopting the optimized fermentation conditions of the auto-induction culturemedium and dual-temperature, the extracellular pullulanase activity can reach 60-70U / mL. The method provides an effective strategy for producing extracellular pullulanase with recombinant E.coli and has great significance in the production process for developing novel recombinant pullulanase in future and application value of pullulanase.

The invention relates to an expression, purification, crystallization method, structure elucidation and the field of application technology of forulic acid decarboxylase, which belongs to the field of molecular biology and microbiology application. The invention obtains the coding area sequence of the key enzyme forulic acid decarboxylase which decomposes forulic acid to generate 4-vinyl guaiacol in Enterobacter sp.Px6-4 by the gene clone method, expresses the enzyme in a large amount in the colon bacillus ( E.coli BL21 ) by the heterogenous expressing technology, obtains the forulic acid decarboxylase pure products by the purification method and the forulic acid decarboxylase protein crystallization by the hanging-drop crystallization process, and grasps the structure and active centre of the enzyme. The invention can obtain a large amount of highly active forulic acid decarboxylase, and provide theoretical guidance for reconstructing engineering bacteria and improving the output of 4-vinyl guaiacol by analyzing the active centre of the enzyme, thus having good potential application.

The invention relates to a diagnostic kit for tuberculosis and mycobacterium tuberculosis infectors, a preparation method and an application method thereof. By using the linker for encoding 15 amino acid (G4S1) 3, the encoding genes (SEQ.ID.NO.6) of a mycobacterium tuberculosis specific antigen Rv3875 and Rv3874 are connected in series, and then inserted into an E. coli expression vector, and the high-efficiency expression and purification for the fusion protein of Rv3875 and Rv3874 in the E. coli are achieved. The recombinant protein at least comprises 8 T cell epipositions which can be used for cell immunity diagnosis, and a diagnostic kit and diagnostic method for a whole blood IFN-Gamma release analysis method are established by taking the protein as the basis and combining the human IFN-Gamma enzyme-linked immunoassay technology, and can be used for the early, specific diagnosis and screening of tuberculosis and mycobacterium tuberculosis infectors.

An deactivated multi-bacterium vaccine for preventing the mastitis of milk cow is composed of Staphylococcus aureus, agalactic streptococcus, milk-stopping streptococcus, breast streptococcus, colibacillus and aluminum hydroxide gel. Its preparing process includes such steps as choosing the pathogenic bacterial strain of said mastitis, preparing seed liquid and reproduction liquid, purity checking, deactivating, preparing vaccine, and discriminating.

The invention relates to a method for the detection of pathogenic food-relevant bacteria, particularly to a method for the simultaneous specific detection of bacteria of the genus Listeria and the species Listeria monocytogenes by in situ-hybridization as well as to a method for the specific detection of bacteria of the species Staphylococcus aureus by in situ-hybridization as well as to a method for the specific detection of bacteria of the genus Campylobacter and the species C. coli and C. jejuni by in situ-hybridization as well as the corresponding oligonucleotide probes and kits, with which the inventive methods may be carried out.

The present invention relates to gene chip for detecting several kinds of common bacterial pathogens, and the gene chip includes solid carrier and oligonucleotide probe fixed on the carrier. The chip has up to 200 low density distributed sample points. The oligonucleotide detecting probe includes DNA' s selected from nucleotide sequences corresponding to the genomes of nine kinds of common bacterial pathogens, including Staphylococcus aureus, Staphylococcus epidermidis, bacillus pyocyaneus, colibacillus, etc. The said chip together with sample treating reagent, hybridizing reagent, color reagent and the specification constitutes the detection kit. The present invention has high detection efficiency.

The invention discloses a heavy metal-tolerant bacterial strain Enterobacter sp.T2 and application thereof. The collection number of the heavy metal-tolerant bacterial strain Enterobacter sp.T2 is CGMCCNo.4281. The heavy metal-tolerant bacterial strain Enterobacter sp.T2 can tolerate various heavy metals such as Pb<2+>, Cr<6+>, Mn<2+>, Zn<2+>, Cu<2+>, Ni<2+>, Cd<2+>, Co<2+>, Ag<+>, Hg<2+> and the like, in particular, the Pb<2+> tolerance concentration and the Mn<2+> tolerance concentration are more than 2,000 mg / L and 1,000 mg / L respectively. A heave metal-removed adsorbent is prepared by activating, culturing, collecting, washing and drying the bacterial strains and the maximum adsorption quantity of the heavy metal Pb<2+> can reach 81.22 mg / g.

The invention discloses compound fresh-keeping liquid for chilled duck meat as well as a preparation and the application of the compound fresh-keeping liquid. Per 100 mL of compound fresh-keeping liquid for chilled duck meat comprises nisin accounting for 0.05 to 0.1g, chitosan mother liquor accounting for 5 to 10g, sodium lactate accounting for 4 to 4.8g, clove leaching solution accounting for 4.2 to 6g, cassia bark leaching solution accounting for 3.6 to 6g, pH conditioning agents accounting for proper quantities, and the rest is water. Secondary fresh-keeping treatment comprising soaking and spraying is adopted, the compound fresh-keeping liquid is applied to antisepsis and fresh preservation of chilled duck meat, and the synergistic action among different antiseptic bacteriostatic agents can be exerted; meanwhile, the double fresh-keeping treatment can inhibit the growth of special putrefying bacteria in chilled duck meat more effectively, such as pseudomonas, lactic acid bacteria, aeromonas veronii, brochothrix thermosphacta, enterobacterium and staphylococcus; and in addition, original quality and color of duck meat can be kept, and the conventional shelf life for chilled duck meat can be prolonged to reach about 10 days.

The invention provides a preparation method of hyaluronic acid. The method comprises the following steps: transforming a recombinant expression vector into the colibacillus to obtain engineering colibacillus by constructing the recombinant expression vector of which hyaluronic acid synzyme gene Has and uridine diphosphate glucose dehydrogenase gene are jointly expressed in the colibacillus; and fermenting the engineering colibacillus to obtain the hyaluronic acid. After the method is used, the production yield of the hyaluronic acid achieves 2.5g / L. compared with the prior art, the yield of the highest HA (hyaluronic acid) obtained by gram positive chained coccus HA synthetic gene spHas expressed in the colibacillus is approximately improved by 10 times.

A reagent kit based on recombinant antigen for detecting Toxoplasma is prepared through taking 542-1218 fragment (t SAG1) from the primary surface antigen gene SAG1 of Toxoplasma, subcloning it to soluble expression carrier pET32a(+), transferring it to colibacillus, configuring engineering bacterium pET32a-tSAG1 / BL21, IPTG induced efficient expression, ultrasonic splitting to obtain supernatant, purifying by Ni-NTA and Sephadex-G75, and coating the microholes on ELISA plate. Its test paper can also be prepared by same way.

The present invention provides an edible film and film solution comprising incorporated organic acids; protein and glycerol useful for coating raw whole fruit, fresh cut fruit, vegetables, meat, poultry, seafood, cereals, nuts, etc. Moreover, the edible films of the present invention can inhibit pathogen growth including Listeria monocytogens, Salmonella gaminara and E. coli 0157:H7. In a preferred embodiment, the edible film comprises 0.9% glycerol; 10% soy protein; and 2.6% malic acid. The present invention also provides a method for coating comestible products with edible films without masking the color but increasing the shelf-life.

The present invention relates to polynucleotide sequences comprising the SEQ ID N° 1 encoding CRM197 and optimised for its expression in E. coli. The invention consequently concerns a method for the production of CRM197 in E. coli via a fusion protein CRM197-tag.

The invention relates to a Cronobacter spp. 16S rDNA gene based sequence, and also provides a method for specific detection of Cronobacter spp. by combining magnetic beads and a nested PCR (polymerase chain reaction) technology. The advantage of the invention lies in that: a specific probe and primers of the Cronobacter spp. 16S rDNA gene sequence are utilized, and magnetic beads are combined to conduct rapid and specific capture and enrichment of a Cronobacter spp. target gene in a sample, finally amplification detection is performed through the nested PCR technology, and the detection sensitivity is improved.

The invention discloses a White Spot Syndrome Virus(WSSV) resisting engineering protein VP28-CD, the preparation and application thereof. The engineering protein VP28-CD is an isolated protein with sequence of ribonucleotide of SEQ ID NO:1 plus the amino acid sequence of SEQ ID NO:2; the bacterial strain is recombinant gene engineering scherichia coli BL21 (pET-32a-VP28-CD), CCTCC No:M208032; White Spot Syndrome Virus(WSSV) is isolated from prawn and gene VP28 is obtained through PCR; silkworm is induced with colon bacillus JM109 to obtain the total mRNA of fat body of the silkworm and the total cDNA is obtained through RT-PCR; mature gene of silkworm antibacterial peptide Cecropin D is obtained through PCR, with the upper reach inserted with aspartic acid-proline at the acid hydrolysis locus; after that recombinant plasmid Pet-32a(+)-VP28-CD is constructed; the recombinant plasmid Pet-32a(+)-VP28-CD is transformed into colon bacillus BL21(DE3); under IPTG induction, the fused protein VP28-CD achieves soluable and high-level expression. The fused protein is applicable in the preparation of the drugs for curing and preventing White Spot Syndrome Virus(WSSV).

The invention provides a hydrogen-bonded rare earth metal complex and a method for preparing the same. The structural general formula of the hydrogen-bonded rare earth metal complex is RExM'yM'z.nH2O. The invention synthesizes and prepares the rare earth metal organic complex by molecular structures. The rare earth metal and the organic ligand complex have cooperative antibacterial function, thus the synthesized rare earth metal organic complex has the advantages of high antibacterial efficiency, wide antimicrobial spectrum, long antibacterial aging, and so on, and has better antibacterial function on colon bacillus, Staphylococcus aureus, and so on, and can be applied to industries of chemical antimicrobial. Moreover, the synthetic method has mild technological conditions, simple equipment and convenient operation.

The invention belongs to the field of biological repair of environmental pollution, and particularly relates to a 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium SE08, and a screening method and application thereof. The 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium SE08 is Enterobacter sp. of which the collection number is CGMCC No.7016 and the collection date is 2012.12.18; and the 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium SE08 is collected at China General Microbiological Culture Collection Center. The invention also discloses a screening method and application of the 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium SE08. The 2-methyl-4-chlorophenoxyacetic acid weedicide degrading bacterium SE08 is obtained by primarily screening in 2-methyl-4-chlorophenoxyacetic acid heavily-polluted soil in the tobacco growing area in Chenzhou City of Hunan Province, rescreening and separation purification; and the degrading bacterium can effectively degrade 2-methyl-4-chlorophenoxyacetic acid residues in the environment, and is applicable to biological repair in 2-methyl-4-chlorophenoxyacetic acid polluted soil and water.

An antimicrobial composition and a method for administering the antimicrobial composition through the water or feed of livestock, wherein the antimicrobial composition is made up of lysozyme and various other agents that act synergistically with lysozyme, such as, dried egg powder, albumen, a sequestering agent and / or a lantibiotic. The composition is used to inhibit the growth of, and diseases and epidemiological significant effects caused by, Clostridium perfinigens, E, coli and Salmonella, in the gut of livestock. More particularly, the antimicrobial composition and method relate to a feed additive that can be administered to poultry and / or swine through their feed. The use of such a feed additive may also inhibit other enteric pathogens that may be present in the gut of livestock.

The invention discloses an ecological gynaecological externally-applied medicine composition and a preparation method thereof. The preparation method of the composition comprises the steps of conducting sterilization on glucose, tween 80, dipotassium phosphate, sodium acetate, seven-molecule crystal water magnesium sulfate, manganese sulfate, ammonium citrate tribasic, soluble starch and yeast cream, inoculating probiotics liquid, and conducting fermentation, so that a lactobacillus fermentation culture is obtained; adding one or more types of medicinally acceptable additives to the culture, so that the ecological gynaecological externally-applied medicine composition is obtained. The ecological gynaecological externally-applied medicine composition can be used for adjusting ecological balance of the external genital tract of a woman and preventing and treating female vaginitis. Experimental results show that the medicine composition has the inhibition and killing effects on staphylococcus aureus, colon bacillus and candida albicans, no irritation is caused to skin, and no skin allergic reaction is found.

The invention discloses Enterobacter mori, and a biotransformation method for a fermented wheat bran extract used for cigarette and an application, which belongs to the biology technical field and cigarette flavor technical field. The invention provides a strain of Enterobacter mori VL4-3CCTCC M2012020. The biotransformation method for fermented wheat bran extract used for cigarette by the Enterobacter mori comprises the following steps: 1)backflow extraction; 2)inclined plane culture; 3) seed culture solution preparation; 4) fermentation; and 5) extract preparation. The invention also provides an application of the Enterobacter mori in biotransformation to prepare the fermented wheat bran extract used for cigarette. According to the invention, the process is simple, environmental protection and harmlessness performances are provided, the raw material cost is low, the product is natural, and the fermented wheat bran extract has industrial prospect. The fermented wheat bran extract for cigarette can be directly used for perfuming on the cigarette and coordinating with flue gas, the fermented wheat bran extract has thick and sweet fragrance, thereby the flue gas is softened, the fragrance is enriched, and the adsorption can be improved.