39 results about "Tear lysozyme" patented technology

The invention discloses a preparation method of an electrochemical sensor capable of simultaneously detecting two acute leukemia markers. The method comprises the following steps: respectively standing a lysozyme report probe and a gamma-interferon report probe in a liquid containing trichloroethyl phosphate (TCEP), so as to open a disulfide bond and respectively form a double-chain structure together with a lysozyme aptamer and a gamma-interferon aptamer; preprocessing a gold electrode, and immersing the processed gold electrode into the pre-processed mixed liquid of lysozyme report probe-aptamer double chains and gamma-interferon report probe-aptamer double chains; standing at room temperature over the night, and then cleaning by using secondary distilled water and a cleanout fluid; immersing the electrode into the liquid containing MCH to seal the electrode, and then cleaning the secondary distilled water and the cleanout fluid; and taking the electrode processed by the above steps as a work electrode to be connected on a chemical work station together with a reference electrode and a counter electrode, so as to obtain the electrochemical sensor. The electrochemical sensor can be applied to simultaneous detection of two acute leukemia markers, namely lysozyme and gamma-interferon.

The present invention provides a method for prophylaxis and therapy of infectious diseases caused by microorganisms present in biofilms adherent to cell surfaces comprising the step of administering to an individual in need thereof a composition comprising a combination of at least one compound chosen from the group of peroxidase, lactoferrin, lactoferrin peptides, lysozyme and immunoglobulins and at least one growth factor.

A composition for prophylactic and / or therapeutic medicinal applications, or plant protection applications, in particular for the control of microorganisms, either planktonic or organized in biofilms. The composition includes at least one ion selected from hypohalite, at least one compound selected from lactoferrin, lactoferrin peptide, lysozyme, immunoglobulins or a combination thereof, optionally hypothiocyanite, and optionally at least one growth factor.

A method is described for the production of wine, comprising the steps of:a) preparing a grape must;b) subjecting said grape must to clarification; andc) subjecting said clarified grape must to alcoholic fermentation to obtain said wine;characterized in that it comprises the step of adding to said clarified grape must, in sequence 1) at least one tannin and 2) a lysozyme. The use of tannin and lysozyme according to the invention enables to eliminate the sulfiting step involved in traditional methods and the sulfite-free wine thus obtained exhibits good chemical and microbiological stability.

The invention relates to a method for performing antibacterial finish to a fabric by immobilizing muramidase, which belongs to the technical field of application of biotechnology to textile. The method comprises the following process steps: (1) the fabric is pretreated, wherein different fabrics adopt different pretreatment methods; and (2) the immobilization of the muramidase is performed, wherein the pretreated fabric is placed in a 5-25g / L muramidase buffer solution (pH7.0) to react for 4 to 16 hours at a temperature of between 0 and 10 DEG C, is taken out to be fully washed by deionized water, and is dried at room temperature for standby. The method is a novel fabric antibacterial finish method, immobilizes the muramidase on textiles through a biological enzyme immobilization technology, and endows the fabric with good antibacterial effect.

An antimicrobial composition and a method for administering the antimicrobial composition through the water or feed of livestock, wherein the antimicrobial composition is made up of lysozyme and various other agents that act synergistically with lysozyme, such as, dried egg powder, albumen, a sequestering agent and / or a lantibiotic. The composition is used to inhibit the growth of, and diseases and epidemiological significant effects caused by, Clostridium perfinigens, E, coli and Salmonella, in the gut of livestock. More particularly, the antimicrobial composition and method relate to a feed additive that can be administered to poultry and / or swine through their feed. The use of such a feed additive may also inhibit other enteric pathogens that may be present in the gut of livestock.

The present invention provides an indicator for the in-situ detection of the presence of a lysozyme or a microbe producing lysozyme at a location. The indicator comprises a layer (8) which is susceptible to degradation by lysozyme and a signaling layer (7) which is adapted to produce a detectable signal which indicates the presence of the lysozyme or the microbe producing lysozyme which is located at substantially the same location as the lysozyme or microbe. In use, the signaling layer is at least initially protected from contact with the lysozyme or microbe which is located at substantially the same location as the lysozyme or microbe by the degradable layer.

The present invention generally features therapeutic and diagnostic compositions and methods for increasing or decreasing the binding of a lysozyme polypeptide to a Treponema pallidum P17 polypeptide (Tp17) or a Tp17-like polypeptide. More particularly, the invention relates to compositions and methods for detecting, treating, or preventing a pathogen infection or a chronic disorder; and to binding assays using a Tp17-like polypeptide and a lysozyme polypeptide.

A composition for removal of biofilm in the airway passage is useful for the treatment of infections such as pneumonia cause by Mycoplasma pneumoniae. In general, the composition comprises: (1) a quantity of at least one enzyme that catalyzes the hydrolysis of a bond that connects two monosaccharides in a polysaccharide or that connects a monosaccharide with a protein molecule in a glycoprotein sufficient to break down biofilm in the airway; and (2) a pharmaceutically acceptable carrier suitable for administration into the airway. The composition can further include ingredients such as a steroid, lysozyme, lactoferrin, or a peroxidase; if a peroxidase is included, the composition can further include an oxidase to generate peroxide as well as a substrate for the oxidase. The composition can be used in methods for treatment of an infection based on the ability of the composition to dissolve biofilm in the airway.

The invention discloses flute diaphragm glue and a manufacturing method thereof. The flute diaphragm glue comprises, by weight, 5-10 parts of donkey-hide gelatin powder, 3-6 parts of gellan gum, 14-32 parts of mucopolysaccharide, 0.2-2.2 parts of mucoprotein, 30-55 parts of deionized water and 10-20 parts of lysozyme. The manufacturing method of the flute diaphragm glue comprises 1, weighing 5-10 parts by weight of donkey-hide gelatin powder, 3-6 parts by weight of gellan gum, 14-32 parts by weight of mucopolysaccharide and 0.2-2.2 parts by weight of mucoprotein, crushing the above materials to obtain granules, pouring the granules into 30-55 parts by weight of deionized water and 10-20 parts by weight of lysozyme, putting all the raw materials into a container, and carrying out stirring at a rate of 800-900rpm to obtain a uniform mixture, and 2, covering the container with a cover, steaming the raw materials in the container for 2-3h until the raw materials form threads, pouring the melt raw material threads into a mold, carrying out cooling curing and packaging the finished product.

The invention discloses a method for preparing a water-replenishing type antibacterial mite-killing paper-based facial mask. The method comprises the following steps: manufacturing a facial mask basecarrier, and preparing an antibacterial agent compos biological enzyme, wherein the facial mask base carrier is prepared by steps of pulping plant fibers as raw materials, molding, drying, coiling, cutting, and the like. Because of porous structures and relatively large wall cavities, medium-fine fibers can be enriched when the plant fibers are pulped, a high porosity rate can be achieved in the molding process, and thus the facial mask base carrier is good in air permeability and high in water retention value; meanwhile, the strength and the water retention value of the facial mask base carrier can be increased by adjusting the pulping degree of the plant fibers, and the phenomenon that an essence drops down and is wasted as a common facial mask in the market is low in water retention value can be effectively alleviated; the plant fibers are natural high polymer compounds, and are stable in component and relatively safe to use; a composite biological enzyme of compounded glucose oxidase and lysozyme is adopted as an antibacterial agent, and the antibacterial agent is a biological antibacterial agent with a specific catalysis capability, is completely harmless to human bodies, is gentle in use environment, has broad spectrum mite-killing and antibacterial effects, has a relatively good skin-care effect and is relatively safe to use.

Provided is a novel agent for promoting osteogenesis and / or inhibiting bone resorption, which: can be directly taken from daily meals by mouth for long periods without any trouble in taste; directly impart a promoting effect on osteogenesis and an inhibiting effect on bone resorption to the bone; and can be expected to have therapeutic effects for preventing or ameliorating / treating various bone diseases. The above agent includes, as an active ingredient, any one or more of β2-microglobulin, histone, complement component 3, monocyte chemotactic protein 1, lysozyme, ribonuclease, prothrombin, cytochrome P-450, plasminogen, transferrin, thrombin, plasmin, complement component 4, and β defensin, and enzymatic digestion products thereof. Those substances have a remarkable inhibiting effect on bone resorption by osteoclasts and / or a promoting effect on osteogenesis via promotion of the proliferation and differentiation of osteoblasts.

Refractile particles containing a heterologous polypeptide as an insoluble aggregate are recovered from bacterial periplasm. The process involves culturing bacterial cells so as to express nucleic acid encoding phage lysozyme and nucleic acid encoding the heterologous polypeptide under separate promoters, disrupting the cells mechanically to release the phage lysozyme so as to release retractile particles from the bacterial cellular matrix, and recovering the released retractile particles from the periplasm. Chloroform is not used in any step and the recovery step minimizes co-recovery of cellular debris with the released retractile particles.

The invention discloses a special water curtain type solidification granulation cylinder device for a lysozyme dimer. The special water curtain type solidification granulation cylinder device mainly comprises a granulation cylinder body with a small cone angle cylinder bottom, an umbrella-shaped liquid flowing device, an atomizing device and a control system, wherein the atomizing device mainly comprises a lysozyme dimmer preparation liquid inlet, a preparation liquid distribution pipe, a compressed air inlet, a gas distribution pipe, a series of atomizing nozzles and a series of connection hoses; the umbrella-shaped liquid flowing device mainly comprises a calcium chloride umbrella-shaped sprayer and an umbrella-shaped disk. When a lysozyme dimer raw material liquid passes through a front fan-shaped mixing atomizing nozzle, micro droplets can be formed and uniformly jet to the umbrella-shaped disk in a fan-shaped manner for granulation; meanwhile, a calcium chloride solution can be guaranteed to form a uniform continuous umbrella-shaped flow layer, the lysozyme dimer raw material liquid is in contact with the uniform continuous umbrella-shaped flow layer to be cured, and cured lysozyme dimer together with the continuous umbrella-shaped flow layer flows into the small cone angle cylinder bottom of the granulation cylinder body, and is then output via a pneumatic cylinder bottom control valve. Therefore, continuous granulation production is realized.

The invention relates to an antimicrobial composition for local use on mucosal membranes and skin. The antimicrobial composition comprises a system of lysozyme and glycosylated immunoglobulins. The glycosylated immunoglobulins have affinity towards gram negative and gram positive bacteria and viruses. Moreover, the invention concerns the method of preparation of said composition as well as use of the composition for the treatment and / or prevention of infections. The present invention further concerns and antimicrobial composition, comprising lysozyme conjugated to a monosaccharide. The method of preparation of said composition as well as use of the composition for the treatment and / or prevention of infections is also described.

Disclosed is habit modified crystals of zinc oxide comprising entrapped particles of protein, wherein said crystals are shaped like a disc and are in the form of spherical assemblies of average size 0.8 [mu]m to 1.5 [mu]m and where the thickness of each disc is 10 nm to 30 nm and average diameter is 200 nm to 500 nm wherein said protein is water-soluble and wherein said protein is at least one ofsericin, bovine serum albumin, gelatin, egg-albumin, casein, zein, whey or lysozyme. Also disclosed is process of making habit modified crystals of zinc oxide comprising the steps of: (i) mixing aqueous solution of a water-soluble protein with aqueous solution of a non- hydrolysable water-soluble zinc salt wherein said protein is water-soluble and wherein said protein is at least one of sericin, bovine serum albumin, gelatin, egg- albumin, casein, zein, whey or lysozyme; (ii) adding a weak base thereto; (iii) heating the reaction mixture of step (ii) to attain a temperature of 60 to 90 DEG C;and, (iv) filtering the contents of step (iii), washing the residue with water and drying it to obtain said habit modified crystals of zinc oxide as the filtrate.

Use of modified lysozyme c or its pharmaceutically acceptable salts and its industrial production for the preparation of medicinal compositions, suitable for oral, parenteral and topical administration, for prophylaxis and therapy of diseases in mammals.

The invention discloses a method for preparing a two-dimensional nano-film of biological protein by using lysozyme. The method utilizes the phase transition reaction of lysozyme molecules and tris(2-carboxyethyl)phosphine hydrochloride to produce nanoparticles at the gas-liquid interface. A layer of biological protein two-dimensional nano film is formed by surface interface induced self-assembly, or the substrate is directly contacted with the surface of the solution during the preparation of the film, so that the nanoparticles generated by the phase transition of lysozyme are directly on the liquid-solid surface through surface interface induction Self-assembly forms a two-dimensional nano-film, that is, grows in situ on the surface of the substrate. The preparation method of the present invention is simple and environment-friendly, the thickness of the prepared biological protein two-dimensional nano-film is about 30-80nm, the surface roughness is small, the transparency is high, and it has good adhesion, and can be used for substrate surface Make modifications.

The invention discloses a fluidized drying device for new feed additive lysozyme dimer particles. The fluidized drying device comprises a hot air inlet bin, a material bin, a particle fluidizing bin and a particle recovery bin which are arranged from bottom to top in sequence and fixed to a fixing frame. The hot air inlet bin is connected with a steam heater. A heating device and a heat insulationlayer are arranged on the periphery of the outer wall of the particle fluidizing bin in sequence. The particle recovery bin is provided with a particle anti-leakage cloth bag and an induced air outlet. The induced air outlet is connected with an air inducing device. The particle anti-leakage cloth bag covers the induced air outlet to allow gas to pass through and prevent dust or particles from passing through. The fluidized drying device provided by the invention controls the temperature of hot air for heating a material to be dried not to be higher than 73 DEG C by controlling the steam technology to prevent lysozyme protein denaturation. The fluidized drying device continuously heats the material to be dried to be fluidized through the air inducing device and continuously takes away water vapor to achieve the effect of effectively and quickly drying the material to be dried, and the generation efficiency is high.

The present invention generally features therapeutic and diagnostic compositions and methods for increasing or decreasing the binding of a lysozyme polypeptide to a Treponema pallidum P17 polypeptide (Tp17) or a Tp17-like polypeptide. More particularly, the invention relates to compositions and methods for detecting, treating, or preventing a pathogen infection or a chronic disorder; and to binding assays using a Tp17-like polypeptide and a lysozyme polypeptide.

The present invention relates to a protein having an activity of giving a lysozyme insensitivity to a lysozyme-sensitive microorganism belonging to Corynebacterium glutamicum; DNA which codes for the protein; a recombinant vector containing the DNA; a transformant obtained by introducing the recombinant vector into a host cell; a bacterium having a lysozyme sensitivity in which the activity of the protein is inactivated; and a method for producing an amino acid using the bacterium.

The invention discloses a method for biomimetic synthesis of nano zirconium dioxide immobilized laccase, wherein the method comprises the following steps: proportionally mixing a lysozyme solution anda laccase solution of certain concentration; adding the mixture into a zirconium precursor solution; shaking to obtain white precipitate; centrifuging, washing and freeze drying to obtain immobilizedlaccase particles. According to the method disclosed by the invention, the operation process is simple and easy to implement, and the reaction conditions are mild; the obtained immobilized laccase has high stability and can be used repeatedly; the defects of traditional immobilization method such as complicated immobilization process, violent reaction conditions, weak combination between enzyme and carrier and low stability of immobilized enzyme are overcome.

Trp-containing peptides, preferably from hen's egg hydrolysate can increase the Cys levels in blood serum. This results in increased keratin production, and enhances growth, appearance, and / or volume in keratin-containing tissue including skin, hair, and / or nails.

The invention discloses a deodorant liquid and a preparation method and application thereof. The deodorant liquid comprises the following components (by weight): 0.05-0.1 part of beta-cyclodextrin, 2-10 parts of lysozyme, 0.5-1.5 parts of citric acid, and the balance water. Further, the deodorant liquid further includes chitosan and / or xylitol. Further, the deodorant liquid includes fragrance. Thedeodorant liquid can eliminate the deficiencies of various methods and products for preventing and treating bromhidrosis. The formula is simple, has no toxic or harmful ingredients, is simple to use,is effective for a long time, and can be used by various groups of people including pregnant women and babies.

The subject invention is directed to the prevention and treatment of respiratory disorders by intratracheal administration of an effective amount of lysozyme, either alone or in combination with other therapeutic agents. Applicable respiratory disorders include, but are not limited to, pulmonary emphysema, asthma, bronchitis, pneumonia, respiratory distress syndrome, bronchopulmonary dysplasia, interstitial fibrosis, cystic fibrosis, and neoplasia. The method is intended for a variety of mammals, including humans ranging from premature neonates to adults.

The invention discloses an odor removing impact bar for closure of an elevator car door, and relates to the field of correlation technique of elevator materials. The odor removing impact bar for closure of the elevator car door is prepared from the following components: ligninase, light calcium carbonate, antimony(III) oxide, camphor, stearic acid, salicylic acid, argil, easy processing channel black, foaming control agent DLF, aromatic essential oil, aloe gel, diatom ooze, activated carbon, nano-modified alkyd resin, impact modifier ACM, polyethylene wax, stearic acid, antiager, butadiene-vinyl pyridine rubber, accelerator TMTM, allicin, superoxide dismutase, lysozyme and matrix rubber. The odor removing impact bar for closure of the elevator car door is efficiently resistant to impact, environmentally friendly, highly resistant to heat, tough, resistant to fatigue, weather, hot water and erosion of alkalis, acids, oils and halogenated hydrocarbon, self-lubricating, low in friction coefficient and highly resistant to hydrolysis, removes odor and has high practical value.

The invention discloses a rapid sterilization device for an ion solidification solution of new feed additive lysozyme dimer. The device comprises a first flow regulating valve arranged in a main pipe,a sterilization liquid cavity, a plurality of ultraviolet sterilization tubes, a pneumatic cleaning regulating valve and a control box, wherein an inlet end and an outlet end of the sterilization liquid cavity are communicated with the main pipe through branch pipes respectively; the plurality of ultraviolet sterilization tubes are arranged on the inner wall of the sterilization liquid cavity; the pneumatic cleaning regulating valve is arranged in the sterilization liquid cavity; the control box is in electrical or signal connection with the first flow control valve, the ultraviolet sterilization pipes and the pneumatic cleaning control valve. In the granulation process, the flowing-through ion solidification solution for solidification of lysozyme dimer particles is sterilized continuously by ultraviolet rays generated by ultraviolet lamps in the process of repeated circulating flowing and using through the pipes at a high speed, microorganisms and bacteria in the solution can be quickly and effectively controlled and killed, and quality of products is improved and conforms to hygienic standards for feed additives issued by the Department of Agriculture of the People's Republic of China.

The present invention relates to a method for the crystallization of protein using nanoparticles as nucleation agents. Precisely, the inventors performed the crystallization of such proteins as Bacillus subtilis YesR, chicken egg white lysozyme, bovine serum albumin, Alicyclobacillus acidocaldarius acetyl-CoA carboxylase, and Listeria monocytogenes hypothetical protein which have his-tag at amino terminal by using gold nanoparticles in diverse sizes and shapes in the presence of Ni2+ ions. As a result, it was confirmed that the chance of successful crystallization was higher with the gold nanoparticles than without the gold nanoparticles and the various crystallization conditions were successfully screened. Therefore, the method for inducing nucleation of the invention can be advantageously used for the disclosure of protein structure by increasing the chance of successful crystallization of protein.

The invention discloses an ion solidifying liquid full-automatic preparation cylinder for lysozyme dipolymer granulation. The ion solidifying liquid full-automatic preparation cylinder comprises a cylinder body, an automatic control platform, a mechanical stirrer, a liquid level sensor, a temperature sensor, a conductometer, a solidifying liquid outlet, a feeding port, a water replenishing pipe, aliquid replenishing pipe and a recycling pipe, a medium jacket filled with a heat conducting medium, a heat-insulating layer and a shell are sequentially arranged on the outer wall of the cylinder body outwards, the medium jacket is provided with a heat conducting medium inlet and a heat conducting medium outlet, a water replenishing electromagnetic valve is arranged on the water replenishing pipe, a liquid replenishing electromagnetic valve is arranged on the liquid replenishing pipe, and the water replenishing electromagnetic valve, the liquid replenishing electromagnetic valve, the liquidlevel sensor, the temperature sensor and the conductometer are electrically connected with the automatic control platform. The preparation cylinder can realize full automation, can accurately controlpreparation of ion solidifying liquid and can recycle the ion solidifying liquid after being granulated, so that production cost is lowered greatly.