Method for antibacterial finishing loomage by immobilized lysozyme

A technology of antibacterial finishing and lysozyme, applied in the application field of biotechnology in textiles, can solve the problems of harm, inability to wash, poor heat resistance, etc., and achieve the effect of expanding applications

Inactive Publication Date: 2009-05-06
JIANGNAN UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because it will be released from the surface of the fiber, these metal substances can be in contact with the skin and penetrate the human body through the pores of the skin, so Hg, Ag and other harmful metal ions have a strong harmful effect on the human body; there are also some inorganic antibacterial agents, although there are Good antibacterial performance, but because it cannot form a firm bond with the fiber, it is not resistant to washing and is gradually eliminated
The main components of organic antibacterial agents are compounds containing reactive functional groups such as quaternary ammonium salts, alcohols, and biguanides. Disadvantages such as short, poor heat resistance and secondary pollution
Natural antibacterial agents come from natural extracts, such as chitosan, juniper alcohol, etc. Its main antibacterial mechanism is considered to be similar to organic quaternary ammonium salts, but the effect is not as good as organic antibacterial agents, and the products are not yet mature

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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  • Method for antibacterial finishing loomage by immobilized lysozyme

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the present embodiment fabric is got woolen fabric, and concrete process step is as follows:

[0022] a. Wool fabric H 2 o 2 Oxidation pretreatment, process prescription and conditions are as follows: H 2 o 2 40mL / L, Na 2 SiO 3 3.5g / L, JFC 1.0g / L, 50°C, pH8.0-8.5, time 35min, bath ratio 1:50. After oxidation pretreatment, it was fully washed with deionized water and dried for later use.

[0023] b. Glutaraldehyde crosslinking: the H 2 o 2 The wool fabric after oxidation pretreatment is cut into small discs with a diameter of 1 cm, immersed in 0.2% glutaraldehyde solution, shaken at a low speed in a water bath constant temperature oscillator at 25°C for 6 hours, and fully washed with deionized water for 3- 4 times, dry and set aside.

[0024] c. Immobilization of lysozyme: put the above-mentioned wool fabric treated with glutaraldehyde in a lysozyme buffer solution (pH 7.0) with a concentration of 5 g / L, react at 4°C for 6 hours, take it out and w...

Embodiment 2

[0031] Embodiment 2: the present embodiment fabric gets cotton fabric, and concrete process step is as follows:

[0032] a. Vector preparation

[0033] Soak 1.0g of bleached cotton fabric in 20mL of 0.40mol / L NaIO 4 solution, placed in a fully enclosed water bath constant temperature oscillator, and reacted at 25-50°C in the dark for 20-60 minutes, then washed with 0.10mol / L glycerin solution for 30 minutes to remove residual NaIO 4 , and then fully washed with distilled water to remove the glycerol, and blotted dry with filter paper for later use.

[0034] b. Immobilization of lysozyme

[0035] The above-prepared carrier dialdehyde-based cotton fabric was placed in a lysozyme buffer solution (pH 7.0) with a concentration of 5 g / L, and reacted at 4°C for 6 hours, then taken out, washed fully with deionized water, and air-dried at room temperature for later use.

[0036] In the present invention, the 2,3-dialdehyde-based cellulose produced by the selective oxidation reaction...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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Abstract

The invention relates to a method for performing antibacterial finish to a fabric by immobilizing muramidase, which belongs to the technical field of application of biotechnology to textile. The method comprises the following process steps: (1) the fabric is pretreated, wherein different fabrics adopt different pretreatment methods; and (2) the immobilization of the muramidase is performed, wherein the pretreated fabric is placed in a 5-25g/L muramidase buffer solution (pH7.0) to react for 4 to 16 hours at a temperature of between 0 and 10 DEG C, is taken out to be fully washed by deionized water, and is dried at room temperature for standby. The method is a novel fabric antibacterial finish method, immobilizes the muramidase on textiles through a biological enzyme immobilization technology, and endows the fabric with good antibacterial effect.

Description

technical field [0001] The invention relates to a method for antibacterial finishing of fabrics by immobilizing lysozyme, and belongs to the technical field of application of biotechnology in textiles. Background technique [0002] Microorganisms play an extremely important role in the material cycle in nature. In the environment of human life, they are everywhere. People deal with microorganisms all the time in their lives and receive the blessings they bestow. At the same time, some Disease-causing microorganisms invade the human body, threatening health and even life. Textiles are the most contacted materials for human beings. In the process of contact with them, human sweat, sebum and other secretions become a good source of nutrition for various microorganisms. Under suitable external conditions (including humidity, temperature, etc.) Under the environment, microorganisms grow rapidly, multiply, and spread diseases through various channels, threatening the survival and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D06M16/00C12S11/00D06M11/50D06M13/123D06M11/30D06M101/12D06M101/06
Inventor 范雪荣王强王平崔莉胡英俊
Owner JIANGNAN UNIV
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