64 results about "Muramidase" patented technology

The invention relates to a preparation method of a protein magnetic imprinting nano sphere, in particular to a preparation method of a protein magnetic imprinting nano sphere which takes one of hemoglobin, muramidase or serum protein as template molecules; the preparation method includes the following steps: synthesizing magnetic nano particles which are Fe3O4 nano particles covered with silicon dioxide on the surfaces; carrying out silanization to the amino-groups on the surface of the magnetic sphere; using glutaric dialdehyde to connect protein; using silylation agent to fix the space structure of template protein; alkaline eluting the protein on the surface of the magnetic sphere to form imprinting sites. The hemoglobin, muramidase or serum protein magnetic nano imprinting polymer sphere prepared by the invention combines the precise and specific identification property of molecular imprinting and the quick separation property of the magnetic nano sphere under the action of an external magnetic field, integrates the advantages of molecular imprinting and the magnetic nano sphere, avoids complex centrifugation operation, plays vital roles in the separation of cells, protein and nucleic acid, the detection of biomolecules, tumor diagnosis and medicine targeting therapy and has promising application prospect in the field of isolation analysis.

The invention relates to a formula of an aquatic product composite preservative. The formula of the aquatic product composite preservative is characterized in that the aquatic product composite preservative in each liter of distilled water comprises the following components: 0.4 to 0.6 g of muramidase, 2.5 to 3.5 g of tea polyphenol, 16 to 20 g of carboxymethyl chitosan, 10 to 14 g of nisin, 25 to 35 g of potassium sorbate, 17 to 23 g of sodium alginate, 20 to 30 g of lactobacillus, 1.5 to 2.5 g of propolis extracts and 10 to 14 g of spice extracts; and the spice extracting liquid comprises the raw materials: galangal, garlic, onion, cinnamon, clove and rosemary. The aquatic product composite preservative provided by the invention is prepared by compounding biological preservatives, is safe and non-toxic, has simple preparation and application methods, prominent microbial inhibition effect and good preservation effect, can greatly prolong the shelf life of frozen aquatic products, and has important industrial value.

The invention relates to a pet staple food capable of enhancing dog disease resistance and regulating stomachic and intestinal microflora, and belongs to the technical field of pet foods. The pet staple food is prepared from dewatered chicken meal, dewatered fish meal, polished rice, bean pulp, wheatmeal, corn flour, monocalcium phosphate, table salt, vitamins, mineral substances, potassium sorbate, lactoferrin, muramidase, whey protein, fructo-oligosaccharide, yeast extracts, glucosamine and attapulgite powder. The pet dog fodder can meet the basic nutritional requirements of dogs, and meanwhile, a proper amount of lactoferrin, muramidase, whey protein and the like are added, so the antioxidant ability and the immunologic function of an organism can be obviously improved; the dog anorexia and apastia phenomena caused by eating a common fodder are improved, and the stomachic and intestinal microflora of the pet dogs can be regulated, so that the pet dogs are healthy in skeleton, bright in hair and healthy in intestinal tract; colon bacillus and intestinal tract toxins of animals can be effectively adsorbed and removed, so the pet staple food achieves the effects of preventing plague, curing diseases, eliminating insects and killing bacteria, enhances the disease resistance of pets, and has a very good overall health care function.

The invention relates to a method for performing antibacterial finish to a fabric by immobilizing muramidase, which belongs to the technical field of application of biotechnology to textile. The method comprises the following process steps: (1) the fabric is pretreated, wherein different fabrics adopt different pretreatment methods; and (2) the immobilization of the muramidase is performed, wherein the pretreated fabric is placed in a 5-25g/L muramidase buffer solution (pH7.0) to react for 4 to 16 hours at a temperature of between 0 and 10 DEG C, is taken out to be fully washed by deionized water, and is dried at room temperature for standby. The method is a novel fabric antibacterial finish method, immobilizes the muramidase on textiles through a biological enzyme immobilization technology, and endows the fabric with good antibacterial effect.

The invention discloses a method adopting linear poly N-isopropyl acrylamide adhesive to assist the renaturation of muramidase in vitro, comprising the following steps that: 1) the linear N-isopropyl acrylamide is prepared by a free radical polymerization method; 2) the linear poly N-isopropyl acrylamide is used to assist the renaturation of muramidase in vitro. The linear poly N-isopropyl acrylamide is taken as a novel protein in vitro additive which can increase the processing concentration of the renaturation muramidase, lower the production cost, and improve the activity recovery rate of the muramidase up to about 80 percent when the processing concentration is 600 Mug/mL; while the activity recovery rate of the dilution renaturation on the same condition is only 10 percent. The gel has the advantages of high efficiency, convenient operation and simple technique, etc., thereby having good potential application in the protein in vitro renaturation field.

The invention discloses a preparation method of a polydopamine/graphene oxide composite film with muramidase immobilized.The method includes the steps that firstly, autopolymerization of dopamine is carried out on the surface of a substrate like glass, an incomplete graphene oxide film layer is introduced onto a polydopamine film layer, part of the zone of the polydopamine film layer is covered with graphene oxide by controlling the concentration of graphene oxide and the temperature of the soaking process, the oxygen-containing functional group part of graphene oxide is reduced by polydopamine at the same time in the process, and a partially-reduced graphene oxide film layer is formed; finally, muramidase is immobilized to the incomplete partially-reduced graphene oxide film layer, and the polydopamine/graphene oxide composite film with muramidase immobilized is obtained.The method is simple and easy to implement; by inserting the incomplete graphene oxide film layer, the enzyme loading capacity of the composite film is obviously increased, the antimicrobial property is remarkably improved, and long-term high-efficiency bacteriostasis can be achieved; the composite film has good application prospects in the fields of food safety, medical treatment and public health and the like.

The invention discloses a muramidase hydrolase external sterile cream for treating skin ulcer and a preparation method thereof. The muramidase hydrolase external sterile cream is prepared from the following raw materials of: muramidase hydrolase, Span, poloxamer, Tween, sorbierite, sodium dihydrogen phosphate, ethylparaben, liquid paraffin, glycerin monostearate, cetyl alcohol, stearyl alcohol, albolene and sterile water. The invention also provides a preparation method for the muramidase hydrolase external sterile cream. The preparation method comprises the following steps of: dissolving; performing high temperature sterilization; filtering and removing bacteria by using a filter membrane; homogenizing at a low speed; homogenizing at a high speed; stirring; reducing temperature; and performing sterile filling. The muramidase hydrolase external sterile cream is used for treating the skin ulcer including bedsore, burn ulcer, calf ulcer, post-zoster ulcer, diabetic ulcer and postoperative ulcer in a transdermal administration form.

A pickled bacon fresh-keeping method utilizing a natural coating liquid is disclosed. The method includes a step of heating 1-10% of sodium alginate and 0.2-0.4% of glycerin under stirring until complete dissolution, performing ultrasonic treatment for 10-60 min to remove bubbles, and keeping the sodium alginate solution for later use; a step of adding 0.1-4% of an acetic acid solution in a chitosan treatment process with the adding amount of the chitosan being 1-8%, performing ultrasonic treatment for 10-60 min to remove bubbles, and keeping the chitosan solution for later use; a step of fully mixing the cooled sodium alginate solution and the chitosan solution, adding 0.01-0.20% of muramidase, and allowing the mixture to stand to obtain the natural coating liquid; and a step of adding preserved bacon slices into the natural coating liquid, soaking the slices for 0.5-5 min, draining the slices off, packaging the slices in vacuum, and preserving the slices at a low temperature, wherein the slices after being packaged in vacuum is preserved at a low temperature that is 8-16 DEG C. The method can achieve a deep-layer fresh-keeping effect, prolongs the shelf life of a pickled bacon product and improves quality of the pickled bacon product.

The present invention provides a high-efficiency extracting method of food-borne pathogen nucleic acid. A PCR technology has been widely used for the detection of pathogenic microorganisms currently. A first step of the PCR detecting technology is the preparation of template nucleic acid, that is, the extraction of nucleic acid in a sample, which directly affects the result of PCR reaction. For a long time, the processes of extraction and purification of the nucleic acid in a sample consume time, and are fussy all the time, so detection speed is seriously slowed down. Thus, a plurality of scholars searches after the extracting method of various nucleic acids. The high-efficiency extracting method of food-borne pathogen nucleic acid comprises the following steps: bacterial culture solution is centrifuged; buffer solution A is added into centrifugal sediment to be vibrated, and muramidase is added; prolease K is added after incubation; buffer solution B is added to be incubated at the temperature of 65 DEG C for 10 minutes after being vibrated violently to be prepared into cracking solution; and high-quality nucleic acid is collected by an adsorption film of silicon matrix SiO2 material is used for extracting nucleic acid solution. The present invention is applied to the detection of the pathogenic microorganisms.

The invention belongs to the field of genetic engineering and fermentation engineering, and provides a human-derived muramidase protein fermentation method. Seed fermentation liquor of human-derived muramidase is added into a fermentation tank for high-density fermentation; high-cell-density fermentation includes methyl alcohol induction, tryptone addition and glycerinum supplement; a BSM fermentation medium is adopted in high-density fermentation of the fermentation tank and is inoculated with seed culture liquor of 5%. The highest activity of human-derived muramidase obtained through the method is 151600 U/ml and 127300 U/ml, the highest protein expression quantity reaches 1.65 g/L, and the batch has high stability. The amplification result is ideal, and practical reference base is provided for industrial production.

The invention relates to a method for screening of chlamys farreri G-type muramidase gene polymorphism markers and the assistant breeding method thereof, belonging to shellfish molecular marker assistant breeding technology in the filed of aquatic organism technology, mainly comprising the steps of: cloning partial sequence of promoter region of a chlamys farreri G-type muramidase gene, preparing disease-resistance population and sensitive population, screening of disease-resistance G-type muramidase gene markers, quickly screening the disease-resistance population and building gene marker assistant breeding technology. According to the invention, promoter region sequence of the chlamys farreri G-type muramidase gene is cloned, and its polymorphism sites are screened. The occurrence frequency of -391 AG individual in disease-resistance population is obviously higher than that in sensitive population, therefore, resistance-related gene marker assistance breeding method is built with -391 AG as chlamys farreri resistance-related G-type muramidase gene marker. The invention has the characteristics of strong pertinence, high breeding efficiency, simple and quick operation, etc., and is suitable for screening of shellfish resistance-related markers and breeding of disease-resistance fine varieties.

The invention discloses enzyme supplementation gum which is composed of 0.1%-0.3% of muramidase, 18%-22% of aluminium hydroxide, 2%-3% of vitamin C, 0.6%-1% of citric acid, 2%-3% of glycerinum, 20%-30% of gum base, 40%-45% of sorbitol, 4%-6% of mannitol, 0.5%-1% of essence and 6%-10% of water. According to the enzyme supplementation gum, the biological muramidase and the citric acid are added, the pH value of the gum can keep the activity of the muramidase to the maximum degree, and bacteria resistance and bacteriostasis, dental calculus prevention, caries prevention, ozostomia relieving, dental plaque inhibition and remarkable improvement of gingival bleeding are conducted on the aspect of biology; the aluminium hydroxide and the vitamin C can assist the muramidase in removing dental plaque and stain, the effect is more remarkable, gingival bleeding is inhibited, and the gum can taste sour and sweet and delicious; the teeth can be healthy and white and breath can be fresh through long-term use of the enzyme supplementation gum.

The invention relates to a composite multienzyme prepared from earthworms and a preparation method thereof. The composite multienzyme comprises various proteolytic enzymes, nuclease, lipoidase, muramidase, phytase, amylase, superoxide dismutase, catalase, cellulose, and the like. The preparation method comprises the following steps: using the earthworms as materials; protecting homogenate at low temperature by a buffer solution; centrifugally separating; washing; precipitating and separating by adding ammonium sulphate; dialyzing to remove SO4; carrying out chromatography by a molecular sieve; and ultrafiltering, concentrating and drying in vacuum at low temperature. The composite multienzyme prepared by purification has the characteristics of high efficiency and multifunction in the aspects of biomacromolecule decomposition and substrate utilization. The preparation method has low cost, and the composite multienzyme has high activity. The functions of all enzymes or partial enzymes of the composite multienzyme can be utilized to achieve a corresponding use aim, thus the composite multienzyme has wide application range.

The invention discloses an anionic framework polymer for extracting muramidase, a preparation method of the anionic framework polymer, and an application of the anionic framework polymer in the muramidase extraction. A special three-dimensional framework structure of existing melamine sponge is used; a framework is wrapped with a chitosan coating; diethylene glycol diglycidyl ether reacts with the chitosan coating, so that the chitosan coating is crosslinked, and the surface of the chitosan coating is provided with suspended epoxy groups; the epoxy groups react with sodium hydrogen sulfite to obtain sulfonate anions; in an ion exchange step in the muramidase extraction, the purification operation is carried out by taking the anionic framework polymer as an integral column, so that the surface of a material and muramidase are subjected to electrostatic interaction, the defect of poor diffusivity of muramidase macromolecules is overcome, the adsorption capacity of the material is improved, and the production efficiency of the muramidase is improved. A framework material has the advantages that the operation is easy and convenient, the repeated use is realized, the cost is low and the efficiency is high.

Aciduliprofundum boonei glycosyl hydrolase 25 (GH25) muramidase is shown here to exhibit antibacterial activity against several distinct bacterial families. Formulations and methods of use for this GH25 are provided.

The invention discloses an industrial preparation method of a dual-functional radish enzyme that is provided with the activity of both chitinase and muramidase and comprises the following steps: firstly raw material is washed and cut, low-temperature induction is implemented to produce the enzyme, homogenate is agitated with a homogenate liquid, a concentrated solution of the dual-functional radish enzyme is obtained through steps of centrifugation, filtering and hyperfiltration and the like, and a protecting agent and a stabilizing agent are respectively added into the concentrated solution, thus obtaining the liquid dual-functional radish enzyme or the dry dual-functional radish enzyme powder. The industrial preparation method of the dual-functional radish enzyme has the following advantages: 1. low-temperature lighting induction technique is adopted, thus raising the raw enzyme content by 2 to 3 times; 2. specific activity stabilizing agent and protecting agent of the dual-functional radish enzyme are developed, thus keeping the activity of the dual-functional radish enzyme basically unchanged in two years at room temperature and reaching the activity of over 97 percent of the raw enzyme; 3. the raw material has wide source and low price, is not affected by factors including season and weather and the like, and can be planted in all seasons; 4. the preparation method has simple technique and low price.

The invention relates to a bio-enzyme corrosion inhibitor for slowing down the carbon steel corrosion in a circulating water system. The bio-enzyme corrosion inhibitor is prepared from the following ingredients by weight portion: 1 portion of lipase and 1.5-2.5 portions of muramidase. The activity of the lipase is larger than 40000u/g, and the activity of the muramidase is larger than 10000u/g. The invention also discloses a method for slowing down the carbon steel corrosion in the circulating water system with the bio-enzyme corrosion inhibitor. The bio-enzyme corrosion inhibitor is formed by compounding the lipase and the muramidase according to a certain mass ratio and has the advantages of less dosage, convenience in use, wide sources, no toxicity, no pollution, good biodegradability and the like. The bio-enzyme corrosion inhibitor can generate a black dense matterl, namely ferroferric oxide on a carbon steel surface to prevent oil leaked from circulating water from further corroding carbon steel equipment. Meanwhile, the lipase in the bio-enzyme corrosion inhibitor can play a role of degrading diesel fuel, and therefore the corrosion inhibition effect is further increased.

The invention of biochemical analysis field relates to a lysozyme testing agent and its manufacture method. The lysozyme testing agent contains killing Micrococcus and gelatin, which is bacteria suspension with high stability, created by new method of killing Micrococcus and using gelatin as medium and stabilizer of disperse thallus. After more than four hours of standing, if absorbance of bacteria suspension does not change notably, agent abtained is proved to have excellent stability and can satisfy various testing requirement, be used in automatic biochemical analysis and realize automatic and quantitive detection of lysozyme activity.

The invention provides a microbiological feed additive. The feed additive contains bacillus coagulans FM603 or a fermentative substance of the bacillus coagulans FM603; the fermentative substance of the bacillus coagulans FM603 contains bacteriocin and has antibacterial activity on gram-positive pathogenic bacteria such as Listeria monocytogenes, staphylococcus aureus, methicillin resistant staphylococcus aureus, clostridium perfringens, clostridium firmus and the like. The molecular weight of the bacteriocin is 4276.45 Da, a partial amino acid sequence is Ala-Gly-His-Dhb-Phe-Val-Dhb-Gly-Pro, and the bacteriocin is more stable under the processing of heat, acid, pepsin or trypsin, is likely to be degraded by pronase and loses activity. With the adoption of the feed additive, the egg laying rate of laying hens can be increased, the feed-egg ratio is reduced, and the egg quality is improved; the feed intake and the daily gain of piglets are increased, and the feed-meat ratio is decreased; the daily gain and the serum muramidase content of broilers are increased, and the feed-meat ratio and the death rate are decreased.

The invention discloses a composition with nutritional and moisturizing efficacies as well as a preparation method and application of the composition. The preparation method comprises the following steps: conducting ultrasonic treatment on chitosan, utilizing muramidase and cellulase for first-stage enzymolysis, and then utilizing chitosanase and pectinase for second-stage enzymolysis; after the enzymolysis reactions are finished, conducting inactivation, decolorization, solid-liquid separation and liquid taking on an enzymatic hydrolysate to obtain a chitooligosaccharides aqueous solution; uniformly mixing a beetroot extract, the chitooligosaccharides aqueous solution, methylisothiazolinone and phenoxyethanol to obtain the composition. The composition has a remarkable effect of nourishing skin and the remarkable capability of promoting the growth of fibroblasts affecting skin aging in the dermis layer; the composition can be utilized for preparation of daily cosmetics, and the market potential of the prepared daily cosmetics is great.

The invention discloses a hyperbranched immobilized metal affinity chromatography stationary phase showed in a general structure formula (I). The hyperbranched immobilized metal affinity chromatography stationary phase and the preparation method thereof have the advantages that a hyperbranching method is used for material surface modification on the surface of the chromatography stationary phase, so that density of material surface functional groups is increased, and high protein adsorption capacity is achieved; the chromatography stationary phase has high metal-chelating chromatographic separation performance, and good separation effect and high protein recovery rate can be achieved when the stationary phase is used for separating muramidase in egg white.