70results about How to "High energy recovery rate" patented technology

The invention relates to a preparation method for magnetic nanoparticle fixed Beta-glucosaccharase, which comprises the steps that (1) precipitation reaction occurs between chloride solution of Fe<2+> and Fe<3+> and NaOH solution to prepare Fe3O4 particles; (2) chitosan is dissolved in acetum to form homogeneous transparent colloidal solution, and Fe3O4 particles are mixed with the homogeneous transparent colloidal solution, and the mixture is beaten, thus getting magnetic nanoparticles; (3) the magnetic nanoparticles are added to Beta-glucosaccharase solution, thus getting chitosan magnetic nanoparticle immobilized enzyme through absorption and cross linkage of glutaric dialdehyde solution; (4) citric acid buffer solution is added to immobilized Beta-glucosaccharase and free Beta-glucosaccharase solution, respectively, and reaction occurs after the temperature is adjusted; and the enzyme activity is examined. The method is quick, simple, convenient and is low in cost. The product got has the characteristics of uniform shape, large specific area, high coefficient of recovery of enzyme activity and so on.

The invention relates to a method of microstructure fixation lipase in the filed of enzyme fixation of biology projection. It dissolves hydrophilic film material acetate fiber into dimethyl form amide to prepare for hydrophilic film solution, it lays water repellency politef film on the glass plate, it spread coats hydrophilic film solution on its surface and uses dry-wet changing method to obtain the compensate film which is formed by fine and close hydrophilic layer and porous bleed layer, it uses filtering method and microstructure to achieve enzyme fixation.

The invention discloses a method adopting linear poly N-isopropyl acrylamide adhesive to assist the renaturation of muramidase in vitro, comprising the following steps that: 1) the linear N-isopropyl acrylamide is prepared by a free radical polymerization method; 2) the linear poly N-isopropyl acrylamide is used to assist the renaturation of muramidase in vitro. The linear poly N-isopropyl acrylamide is taken as a novel protein in vitro additive which can increase the processing concentration of the renaturation muramidase, lower the production cost, and improve the activity recovery rate of the muramidase up to about 80 percent when the processing concentration is 600 Mug/mL; while the activity recovery rate of the dilution renaturation on the same condition is only 10 percent. The gel has the advantages of high efficiency, convenient operation and simple technique, etc., thereby having good potential application in the protein in vitro renaturation field.

The invention relates to a method for preparing immobilized adenosylmethionine synthetase and adenosylmethionine. The method has the following technical characteristics of: crosslinking amino resin carrier Seplite LX-1000HA and glutaraldehyde solution; and reacting the amino resin carrier crosslinked with glutaraldehyde with solution of recombinant adenosylmethionine synthetase to obtain the immobilized adenosylmethionine synthetase. The amino resin carrier has high protein fixation rate, the recovery rate of the enzyme activity of the immobilized adenosylmethionine synthetase is high, and the stability and continuous operation performance are good. When the immobilized adenosylmethionine synthetase is used for producing the adenosylmethionine, the reaction operation is simple, the production cycle is short, and products are easy to purify; the immobilized enzyme can be recycled, the production cost is greatly reduced, the production efficiency is improved, and a new way is provided for industrialized production of the adenosylmethionine.

The invention discloses a control method of the maximum energy recovery when an electric vehicle brakes and belongs to the technical field of electric vehicles. The control method is characterized by comprising the following steps that (1), a calculation decision module of a vehicle control unit calculates needed brake torque according to the current state of the vehicle and the current road state, and the obtained brake torque is analyzed and processed according to the following situations; (2), according to the analysis result, through the coordinated logic control over the brake torque according to the brake process, the control over the maximum energy recovery when the electric vehicle brakes is finished finally. According to the control method of the maximum energy recovery when the electric vehicle brakes, the utilization rate of recovered energy of the electric vehicle is improved to be 50% from 20%, the driving range is increased by 75%, and the safety performance is ensured when the electric vehicle brakes; the road state can be sensed dynamically, the brake requirement by a driver is met in real time, the brake torque is adjusted, the brake requirement of the vehicle is met, and safety is ensured.

The invention relates to a method for preparing magnetic immobilized phospholipase A1 and a method for refining crude oil by using the magnetic immobilized phospholipase A1. The invention aims to solve the problems of complicated separation and recycling of immobilized enzyme and low activity recovery of the conventional method for preparing the immobilized phospholipase A1, and the problem that the magnetic immobilized enzyme is not used for refining the crude oil at present. The method for preparing and refining the crude oil comprises the following steps of: 1, preparing the magnetic immobilized phospholipase A1; 2, heating the crude oil, and adding citric acid solution; and 3, cooling, regulating the pH value, adding the magnetic immobilized phospholipase A1, stirring, and separating the magnetic immobilized phospholipase A1. The recovery rate of the prepared magnetic immobilized phospholipase A1 is over 85 percent, and the phosphorus content of the crude oil is reduced to 7-10mg/kg. The invention is applied to the field of oil degumming.

The invention discloses chitosan microspheres for enzyme immobilization and a preparation method and application thereof.The preparation method comprises the steps that an acetic acid solution with the mass fraction of 5% is added to 0.5 g of chitosan powder, liquid paraffin is added after the chitosan powder is sufficiently dissolved, and stirring is conducted for 10 min; the temperature is increased to 50 DEG C, an emulsifier Span80 is dropwise added, and emulsification is conducted for 10 min; 0.5-2.0 mL of formaldehyde solution is added, and a stirring reaction is conducted for 1.5 h; the temperature is increased to 70 DEG C, a NaOH solution with the mass fraction of 10% is dropwise added, 1.0-2.5 mL of epoxy chloropropane is slowly and dropwise added on the condition that the pH value of the solution is kept alkaline, and filtering is conducted after a reaction is conducted for 5 h; an obtained product is washed with distilled water and then sequentially washed with petroleum ether, absolute ethyl alcohol and water, the product is subjected to heating treatment with hydrochloric acid of l mol/L for 9 h and then sequentially subjected to alkali washing, acid washing and water washing till the pH value of washing liquid is neutral, the product is dried to a constant weight, and the chitosan microspheres (NCTS) for enzyme immobilization are obtained.The chitosan microspheres are good carriers for immobilizing enzymes.

The method for assisting lysozyme renaturation with temperature-sensitive poly N-isopropyl acrylamide gel includes the following steps: 1) compounding poly N-isopropyl acrylamide gel aqua; 2) preparing poly N-isopropyl acrylamide gel in reverse suspension process; and 3) assisting lysozyme renaturation with temperature-sensitive poly N-isopropyl acrylamide gel. The temperature-sensitive poly N-isopropyl acrylamide gel the present invention develops as one novel additive for extracorporeal renaturation of protein can raise the lysozyme activity recovering rate. The gel has high efficiency, less renaturation affecting parameters, convenient operation, relax requirement in instrument and apparatus, easy separation and recovery and other advantages. The present invention has excellent application foreground in extracorporeal renaturation of protein.

The invention relates to an immobilized lipase which takes fasciculus vascularis luffae as a carrier and preparation of the immobilized lipase. In the immobilized lipase, the mass ratio of the carrier lipase and the fasciculus vascularis luffae is between1:4 and 1:8. The concrete preparation method comprises deoiling treatment of the fasciculus vascularis luffae, refinement of the lipase and immobilization of the lipase. The invention takes the fasciculus vascularis luffae as the carrier for immobilizing the lipase, thereby the immobilized lipase obtained has high activity recovery rate and high repeated utilization ratio and can be used for a plurality of times without deactivation. Moreover, the technological method is simple in operation and has a chance of promoting the industrial progress for enzymatical production of biological diesel fuels.

The invention relates to a zwitter-ion magnetic composite hydrogel immobilized enzyme supporter and a preparation method. The immobilized enzyme supporter is zwitter-ion hydrogel in which Fe3O4 nanoparticles are embedded; one end of glutaraldehyde, which has a bifunctional group, is connected to the zwitter-ion hydrogel, while the other end forms Schiff base with amino groups on the surface of theenzyme, so that the enzyme is covalently connected to the surface of the hydrogel supporter. The preparation method includes the steps of: mixing a zwitter-ion monomer, a crosslinking agent, an initiator and a solvent to obtain a liquid mixture, and mixing the liquid mixture with a Fe3O4 solution according to volume ratio of 3:5-3:10; and performing ultraviolet radiation, or heating or normal temperature treatment to the mixture solution containing Fe3O4 so that the Fe3O4 is embedded in the monomer via crosslinking polymerization, thereby forming the zwitter-ion magnetic composite hydrogel immobilized enzyme supporter in which the Fe3O4 is embedded. The enzyme immobilization method inherits the advantages in conventional covalent combination enzyme immobilization methods. The method is high in recovery rate of enzyme activity and has high application value.

The invention discloses a method for flexibly immobilizing papain by using modified polyacrylonitrile resin, which comprises the following steps: 1) preparation of a flexible carrier: modifying polyacrylonitrile resin microspheres by use of tetraethylenepentamine to obtain a modified polyacrylonitrile flexible carrier; 2) activation of the carrier: soaking the modified polyacrylonitrile flexible carrier in a phosphate buffer solution, and performing crosslinking reaction by using glutaraldehyde to obtain a glutaraldehyde modified polyacrylonitrile flexible carrier; 3) preparation of immobilized papain: performing immobilization reaction between the glutaraldehyde modified polyacrylonitrile flexible carrier and papain to obtain immobilized papain. The method disclosed by the invention overcomes the enzyme activity loss caused by rigid collision in the enzyme immobilization process of the carrier synthesized by the prior art, and can maintain the homogeneous catalysis activity of free enzymes to the greatest degree so as to solve the problem of low recovery rate of the immobilized enzyme activity of a covalent binding method.

The invention discloses a method for preparing a liquid-state chymosin preparation. The method comprises the following steps: (1) preparing fermentation liquor by taking bacillus amyloliquefaciens CCTCC NO:M2011045 or bacillus subtilis CCTCC NO:M2010259 as a fermentation strain; (2) performing plate-frame pressure filtration, and performing solid-liquid separation; (3) performing montmorillonite adsorption; (4) performing microfiltration; (5) performing ultrafiltration and concentration; and (6) adding cane sugar or trehalose with the final concentration of 20-40 weight percent, glycerol or sorbitol with the final concentration of 0-30 weight percent and calcium chloride with the final concentration of 0.01-0.05 weight percent into a concentrated enzyme solution, uniformly mixing, and preparing the finished product. According to the method, the chymosin is high in activity and recovery rate, protease, other impurities and residual bacteria in the enzyme solution can be effectively removed, and the prepared enzyme preparation has high quality and stability and is high in enzyme specifity, long in retention period, safe and non-toxic.

The invention relates to an integral rotational radiation preheating mixing energy utilization device with flue gas chilling. The device comprises a gasification furnace body and a heat recovery unit, which are connected with each other through a flange; the heat recovery unit comprises a pressurized shell, a syngas inlet, a radiation heat exchange component, a flue gas chilling component, a convection heat transfer component, an attemperator component, a slag tank and a syngas outlet; the gasification furnace body is communicated with the heat recovery unit through the syngas inlet; syngas is subjected to radiation heat exchange and then chilling for temperature reduction, and then liquid slag falls into the slag tank for discharge; and the syngas reversely flows up, and is subjected to convection heat transfer for temperature reduction. The device integrates radiation heat exchange, flue gas chilling and convection heat transfer effectively, and can be used for an IGCC (Integrated Gasification Combined Cycle) power generation system, the sensible heat of crude syngas is absorbed to produce high-pressure steam or medium-pressure steam to generate power, the entire energy utilization rate is increased greatly, the energy recycling rate is high, the overall dimension of a waste-heat recovery boiler is reduced effectively, the manufacturing, transporting and installing are more convenient, and the problem of dust stratification on convection heat transfer surface can be eliminated.

The invention relates to a mixed energy utilization device for an integrated rotary radiant boiler preheating boiler. The device comprises a gasification furnace body and a heat recovery device, wherein the gasification furnace body is arranged above the heat recovery device; the heat recovery device comprises a synthetic gas inlet, a radiation heat exchange component, a slag bath, a convection heat exchange component and a pressure shell; the synthetic gas inlet is positioned on a seal head on the top of the pressure shell; the radiation heat exchange component is positioned on the upper part of the slag bath; the slag bath is positioned on the upper part of convection heat exchange component; the radiation heat exchange component, the slag bath and the convection heat exchange component are positioned in the pressure shell into a whole; a lower slag port of the gasification furnace body is connected with the top of the heat recovery device through a flange; and the lower slag port is communicated with the synthetic gas inlet. The sensible heat of the gasified coarse synthetic gas can be effectively absorbed, and the generated high-pressure steam or medium-pressure steam is used for power generation or preheating other working media, so that the energy utilization rate is greatly improved, and the energy recycling efficiency is high; and moreover, the overall size of a waste heat boiler is effectively reduced, the device is convenient to manufacture, transport and mount, and the dust accumulation problem in the convection heat exchange area can be eliminated.

The invention relates to a SiO2 based macroporous material immobilized laccase and a preparation method thereof, and belongs to the technical field of immobilized laccase preparation. The immobilized laccase is prepared by fixing laccase on a carrier through a static and coordination effect, wherein the carrier is a SiO2 macroporous block material whose surface is modified. The preparation method comprises two steps namely carrier preparation and laccase immobilization, wherein the carrier preparation comprises the following steps: modifying a SiO2 macroporous block material by sodium aluminate under a hydrothermal condition, and then carrying out ion exchange to obtain a finished carrier product containing multivalent metal ions on the surface; and the laccase immobilization comprises the following steps: soaking the prepared carrier in a laccase solution, carrying vibration absorption at a room temperature, washing, and drying so as to obtain the finished immobilized laccase product. The provided immobilized laccase has the characteristics of simple preparation technology, low cost, high activity recycling rate, and stable property.

The invention discloses a method for assisting the in vitro renaturation of lysozyme by using granular poly (N-isopropylacrylamide-sodium acrylate) copolymer gel, the specific steps are as follows: 1) the granular poly (N-isopropylacrylamide-sodium acrylate) copolymer gel is prepared by using the inverse suspension copolymerization; 2) the granular poly (N-isopropylacrylamide-sodium acrylate) copolymer gel is used for assisting the in vitro renaturation of the lysozyme. The granular poly (N-isopropylacrylamide-sodium acrylate) copolymer gel which is developed by the method is taken as a novel protein in vitro renaturation additive, thereby having the following advantages: (1) the activity recovery rate of a target protein can be significantly improved under the condition of high denatured protein concentration; (2) the separation and the recovery are convenient by utilizing the thermosensitive characteristic of the gel after the completion of the renaturation, and the gel can be repeatedly utilized; (3) the hydrophobic performance of the gel can be regulated by controlling the content of the co-monomer sodium acrylate, thereby changing the low critical dissolution temperature and the largest swelling ratio and being applicable to the different target proteins. Therefore, the gel has better application prospect in the field of the protein renaturation.

The present invention relates to a method for preparing a block macroporous composite immobilized laccase, belonging to the technical field of preparing of an immobilized enzyme. The immobilized laccase is prepared by fixing laccase onto a carrier by physical adsorption method, wherein the block macroporous composite constructed by a silica / chitosan ultrathin membrane is used as the carrier. The preparation method comprises the steps preparation of the carrier and fixing of the laccase. The preparation of the carrier is as follows: chitosan is adsorbed on SiO2 macroporous material pore wall surface, and then the finished carrier, constructed by the silica / chitosan ultrathin membrane, is prepared by crosslinking of chitosan macromolecules by use of a bifunctional crosslinking agent; the fixing of the laccase is as follows: the carrier is immersed in a laccase solution and shaken for reaction at room temperature, and finished immobilized laccase can be obtained by washing and drying. The present invention provides a novel immobilized laccase and a preparation method thereof, and the novel immobilized laccase has the characteristics of simple preparation process, low cost, high immobilized laccase activity, and stable property and the like.

The invention relates to immobilized lysine decarboxylase, a preparation thereof, a 1, 5-pentane diamine preparation method and a product. The invention specifically relates to immobilized lysine decarboxylase comprising a diazo group-modified polymer carrier and lysine decarboxylase, and a method for preparing immobilized lysine decarboxylase by the diazo group-modified polymer carrier; a methodfor preparing 1, 5-pentane diamine by the immobilized lysine decarboxylase, and the prepared 1, 5-pentane diamine thereof. The immobilization method has high enzyme immobilization efficiency and goodstability in use, improves the use efficiency of enzyme, solves the problem of poor use stability of free lysine decarboxylase (cells) in traditional processes, greatly reduces the use cost of the carrier and the biological production cost of 1, 5-pentanediamine, simplifies the separation step of 1, 5-pentane diamine solution and enzyme, increases the degree of automation of the production of 1, 5-pentane diamine, and promotes the process of industrialization of biological production of 1, 5-pentanediamine.

The invention discloses a method for preparing xylo-oligosaccharide and xylose with genetic engineering co-immobilized xylan degradation enzyme. The method comprises the following steps of: firstly, applying an affinity label which has high bonding strength at high temperature and is efficient and stable to the xylan degradation enzyme by a gene recombination technology and then co-expressing, and preparing the genetic engineering co-immobilized xylan degradation enzyme by specifically combining with an immobilized carrier material; and then hydrolyzing a xylan solution or a steam explosion liquid of agricultural and forestry waste by utilizing the co-immobilized xylan degradation enzyme, thereby obtaining xylo-oligosaccharide and xylose. Through the method, one-step method production of multi-enzyme preparation is realized, multi-enzyme purification and immobilization are synchronous, the separation and recycling of products and repeated using of enzyme are coupled, and the stability of enzyme to temperature and pH is improved; and the synergistic effect of the series of xylan degradation enzymes is achieved, the catalytic efficiency is high, the reaction by-products are less, the yield and the purity are high, an the used carrier is polysaccharide which is inert, cheap and easy to obtain, has good biocompatibility, and is especially suitable for the fields of food, medicine and the like.

The invention relates to a preparation method of blocked macroporous material immobilized laccase, and belongs to the technical field of the preparation of immobilized enzyme. The immobilized laccase adopts a blocked SiO2 macroporous material with surface functionalized by virtue of an epoxy group as a carrier, and the laccase is immobilized on the carrier by virtue of a covalent binding method. The preparation method comprises the following two steps: preparation of the carrier and immobilization of the laccase. During the preparation of the carrier, gamma-(2,3-epoxy propoxy)propyl-trimethoxysilane (GPTMS) is adopted as a silane coupling agent, and the GPTMS has grafting reaction on the inner surface of a pore of the SiO2 macroporous material under the solvent thermal reaction condition to prepare the epoxy-group functionalized carrier which can be covalently bound with the laccase. During the immobilization of the laccase, the carrier is immersed in a laccase solution, the solution is vibrated at a room temperature to have the reaction, and a reaction product is washed and dried to obtain the immobilized laccase finished product. The novel immobilized laccase has the characteristics that the preparation process is simple, the cost is low, the activity of the immobilized laccase is high, and the like.