147results about How to "Immobilization" patented technology

A composite adsorbent includes a shaped substrate having an adhesive film on a portion of at least one side and an adsorbent that has been immobilized as a layer on the adhesive portion of the substrate. The composite adsorbent is designed to be shaped into a form that provides a high adsorption capacity, low pressure drop, high volume capacity, and fast adsorption kinetics to effectively adsorb odors over an extended period of time. Optionally, the composite adsorbent is also used in combination with an air permeable unit wherein it is advantageously positioned to provide enhanced adsorption of contaminants found in gas phase.

The invention discloses a biomass-based nanometer lanthanum oxide dephosphorization composite adsorbent and a preparation method thereof. According to the composite adsorbent, biomass anion exchange resin is taken as a matrix, and nanometer lanthanum oxide is loaded on the biomass anion exchange resin; the load of the nanometer lanthanum oxide is 0.5 to 22.6 percent by weight percentage of lanthanum; the biomass anion exchange resin is alkali quaternary ammonium group-contained biomass anion exchange resin, and is prepared by performing aminated modification on a biomass material by taking N,N-dimethylformamide as solvent, epichlorohydrin as an etherifying agent and trimethylamine or triethylamine or urea as an aminating agent; the biomass material is selected from maize straw, soybean straw, cassava straw, rice straw, wheat straw, bamboo reed, saw dust or a mixture thereof. The composite adsorbent disclosed by the invention is low in cost and high in adsorption capacity.

The invention discloses a blocking system and a blocking method for channeling inhibition in carbon dioxide non-miscible flooding of a low permeability reservoir. The system is mainly prepared from the following raw materials in percentage by weight: 1%-2% of fine mineral particles, 0.2%-0.3% of polymer, 0.01%-0.02% of compound crosslinking agent, 0.04%-0.1% of retarder, 0.01% of deoxidant, 0.1%-0.5% of surfactant and the balance of water. By virtue of monitoring the steam injection pressure of a wellhead in real time and adjusting the system, graded control of high-pressure steam injection, 'gas channeling' and 'escape channeling' processes in the carbon dioxide non-miscible flooding is realized and the development effect of the carbon dioxide non-miscible flooding of the low permeability reservoir is improved.

The invention discloses a bifunctional group ion liquid, which comprises the following steps: hydroxy and its derivant group, quaternary ammonium salt cation of sulfonic group and anion of sulfonic acid group or sulfate radical. The ion liquid is stable to meet water with fitful viscosity and low cost, which creates condition for further modified or fixed ion liquid.

The invention relates to a cyclodextrin pillared hydrotalcite film which grows in a way of being perpendicular to a basis and a preparation method thereof, belonging to the technical field of organic-inorganic composite materials and preparation thereof. The chemical formula of pillared hydrotalcite is [(M<2+><1-x> (M<3+><x> (OH)2<x+> (CMCD <n->) x / n question mark mH2O. The preparation method comprises the steps as follows: aluminium flakes are firstly anodized under the condition of constant voltage; divalent metal salts are taken and weighed respectively, cyclodextrin decorated with negative ion and NH4NO3 are dissolved in de-ionized water to prepare into reaction synthetic solution, and then ammonia is used for adjusting the pH of the reaction synthetic solution to a value between 5.9 and 8.5. Anodized alumina / aluminium-base flakes are vertically hung in the reaction synthetic solution to carry out reaction at certain temperature for a certain time to prepare the cyclodextrin pillared hydrotalcite film. The invention has advantages that: the cyclodextrin pillared hydrotalcite film prepared by an in-situ growth technology is fixed on an aluminium basis, thus realizing the immobilization and unitization of the hydrotalcite; besides, the hydrotalcite prepared has certain mechanical strength, which provides great convenience to the application of cyclodextrin pillared hydrotalcite materials both in production and life.

A hydrotalcite composite luminescent material with rhodamine intercalation, which pertains to abio-organic composite luminescent material. The process is preparing solution A with mole ratio of bivalence and tervalence cation M2+ / M3+=2.0-4.0; adding laser dye sulphonated rhodamine and sodium salt of dodecylbenzene sulfonic acid into mixed solution of ethanol and water and fully dissolving to obtain solution of sulphonated rhodamine and dodecyl benzene sulfone acid group containing anion with negative charge B; mixing solution A, B into solution C, preparing NaOH solution; communicating solution C and NaOH solution into full-back-liquid mixing membrane reactor to react, mixing to obtain red slurry, introducing the red slurry into hydrothermal kettle, reacting, absterging by CO2, de-ionized water and absolute ethyl alcohol and centrifugally separating, vacuum drying. Then hydrotalcite system with co-intercalation of suphonated rhodamine and dodecyl sulfone acid group is obtained. Advantages of the invention are that dye molecular is immobilized, and fluorescence quenching caused by dye aggregation is reduced.

The invention discloses a preparation method of a lasting hydrophilization modified polyvinylidene fluoride (PVDF) ultrafiltration membrane. The preparation method of the lasting hydrophilization modified PVDF ultrafiltration membrane comprises the following steps: 1) preparing a film casting solution; 2) preparing a crosslinked blend membrane. After liquid phase blending modification is carried out on PVDF and an organic hydrophilic macromolecule PVA (polyvinyl alcohol), further anhydrous ferric chloride is added into the film casting solution, and PVA is fixed through a complexing reaction carried out on a hydroxyl on a molecular chain of the PVA and Fe<3+>, so that lasting hydrophilic modification of a membrane is realized, and water permeability stability is improved while a membrane structure is optimized; after modification, performances of the membrane are shown as follows: a contact angle is reduced by 30-40 degrees, flux is increased by 1-3 times compared with the flux before modification, and strength is increased by 1-1.5MPa; the anti-pollution capacity of the membrane is shown as follows: after filtering is carried out for 2 hours, the recovery rate of the flux of the membrane is increased by 10-20%, and the attenuation rate of the flux of the membrane is reduced by 5-10%.

The invention belongs to the field of organic-inorganic composite materials and relates to layered double hydroxides (LDHs) loading highly-dispersed fullerenes and a preparing method of the LDHs. The method of the LDHs includes the steps of firstly preparing precursors of the LDHs, then preparing naphthylacetic acid intercalated LDHs, forming a hydrophobic environment containing a plurality of naphthalene groups in layers of naphthylacetic acid intercalated LDHs, and finally fixing the fullerenes into the layers of the LDHs. According to the LDHs loading the highly-dispersed fullerenes and the preparing method of the LDHs, the fullerene and naphthylacetic acid intercalated LDHs are of supramolecular structures, crystal structures of the LDHs are the crystal structures of layered double hydroxide-like materials, and the chemical formula is [ (M2+)1-x(M3+)x(OH)2 ]x+(NAA-)x(C60)y-water(1 / m), wherein x=0.25-0.33,y=0.05-0.1, m=3-6, the m relates to the number of crystal water molecules in the layers, M2+ relates to a divalent metal ion, and M3+relates to a trivalent metal ion of Al3+. According to the LDHs loading the highly-dispersed fullerenes and the preparing method of the LDHs, due to high interaction forces between the fullerenes (C60) and the naphthylacetic acid, the prepared fullerene and naphthylacetic acid intercalated LDHs have stable intercalation structures and can be used for preparing non-linear optics materials, strong magnetic materials and the like, and the preparing method is simple, practicable and easy to operate.

The invention relates to a method of microstructure fixation lipase in the filed of enzyme fixation of biology projection. It dissolves hydrophilic film material acetate fiber into dimethyl form amide to prepare for hydrophilic film solution, it lays water repellency politef film on the glass plate, it spread coats hydrophilic film solution on its surface and uses dry-wet changing method to obtain the compensate film which is formed by fine and close hydrophilic layer and porous bleed layer, it uses filtering method and microstructure to achieve enzyme fixation.

The invention discloses a composite luminescent film containing rare earth polyacid and brucite and a preparation method thereof and belongs to the technical field of the preparation of inorganic composite luminescent films. The preparation method comprises the following steps of: preparing brucite colloidal solution stripped by using a formamide solvent; confecting rare earth polyacid-containingsolution; and performing a plurality of alternate assemblies in two types of solution by using a hydrophilically-treated substrate to obtain a composite multilayer luminescent film containing the rare earth polyacid and the brucite. In the preparation method, sequential assembly of rare earth polyacid-containing functional molecules and brucite nanosheets is realized for the first time, and the application field of a material containing the rare earth polyacid and the brucite is expanded. The thickness of the obtained film is accurately controllable in nanoscale. Simultaneously, the space limiting function of the brucite and coaction between a host and a guest are utilized, so that the immobilization of luminescent molecules containing the rare earth polyacid is realized, the controllableluminous intensity of the film is realized by changing assembly times, the thermal stability of the film is enhanced effectively, and fluorescent quenching caused by aggregation is reduced.

The invention discloses blue light emitting materials of tri(8-hydroxyquinoline-5-sulfonate) aluminum complex anion intercalated hydrotalcite and a preparation method thereof, and belongs to the technical field of inorganic / organic composite luminescent materials. In the method, a surfactant of which the molecular length is similar to that of tri(8-hydroxyquinoline-5-sulfonate) aluminum complex anions is selected so as to obtain the composite luminescent material, dispersed uniformly among hydrotalcite layers, of the tri(8-hydroxyquinoline-5-sulfonate) aluminum complex anion intercalated hydrotalcite by a coprecipitation method. The method realizes the immobilization of the tri(8-hydroxyquinoline-5-sulfonate) aluminum complex anions, improves the heat stability of luminescent molecules of the metal complex and realizes the conversion from the green light luminescent material tri(8-hydroxyquinoline-5-sulfonate) aluminum to the blue light luminescent materials of hydrotalcite intercalated products thereof.

The invention discloses a treatment method for black foul water. The method comprises the steps: (1) overturning bottom sludge of the black foul water so as to form a sludge-water mixed area; (2) pumping muddy water of the sludge-water mixed area, and separating pumped bottom sludge from water; (3) adding a solidifying agent into the bottom sludge separated out in the step (2), carrying out stirring and mixing, solidifying N and P in the bottom sludge, and returning the solidified bottom sludge to a river; and (4) carrying out purifying treatment on the water separated out in the step (2), and returning the purified water to the river. The invention further discloses shipborne equipment for treating the black foul water.

The invention discloses a 4-hydroxy coumarin intercalated hydrotalcite blue-light composite luminescence material and a preparation method of the 4-hydroxy coumarin intercalated hydrotalcite blue-light composite luminescence material, belonging to the technical field of an inorganic / organic composite luminescence material. A surfactant with the molecular length similar with that of a neutral molecule of 4-hydroxy coumarin is selected, and a supermolecular composite luminescence material which is uniformly dispersed between hydrotalcite layers is obtained by co-intercalation of a co-precipitation method. According to the invention, space area-restricting action of a hydrotalcite stratified material and interaction between a subject and an object are utilized; immobilization of the neutral molecule of the 4-hydroxy coumarin is achieved; meanwhile, the conversion of emission into blue-light emission is achieved; and the fluorescence of a neutral fluorescent micromolecule is effectively improved, and a theoretical research foundation is provided for application of the hydrotalcite into a solid luminescence apparatus.

The invention relates to transglucosidase, its preparation method and a immobilization method, which belong to the fields of enzyme engineering and fermentation engineering. More specifically, the invention provides the following processes: 1) Aspergillus niger BLB-16 is screened from soil as an original strain, processes of mutation treatment, optimization and screening are performed to obtain an optimized bacterial strain (BLB-28) for fermenting; 2) a fermentation broth is carried out a heat sterilization to obtain transglucosidase liquid; 3) transglucosidase liquid is performed a nanofiltration to concentrate; 4) processes of resin adsorption, sodium alginate entrapment, immobilization by a glutaraldehyde cross-linking method are carried out for preparing immobilized enzymes. The prepared transglucosidase is suitable for an application in the industrial fields such as foodstuff, medicine, feed and the like, and used for producing isomaltose hypgather.

An immobilized protein catalyst is prepared by applying an adhesive to a polymeric support, applying a layer of a globular protein over the layer of adhesive, binding a crosslinking agent to the protein layer, and binding the protein catalyst by reaction with the crosslinking agent.

A composite adsorbent includes a substrate to which an adsorbent material has been immobilized. The composite adsorbent is designed to be shaped into a variety of forms that provide a high adsorption capacity, low pressure drop, high volume capacity, and fast adsorption kinetics to effectively adsorb contaminants. Optionally, the composite adsorbent is also used in combination with an air permeable unit wherein it is advantageously positioned to provide enhanced adsorption of contaminants found in gas phase.

The invention which relates to a preparation method of a flower ball-shaped micro-nano film photocatalytic material belongs to the technical field of environment chemical engineering photocatalytic water processing. The method which adopts a process combining alcoholysis with coating and allows a BiOCl film to be prepared on a titanium plate matrix comprises the following steps: 1, treating BiCl3 as a raw material and an alcohol as a solvent, adjusting the pH value by ammonia water, and reacting for 1-2h at 40-80DEG C to prepare a liquid precursor; and 2, coating the liquid precursor on the titanium plate matrix, drying, and washing to prepare the flower ball-shaped micro-nano film photocatalytic material having the diameter of 1-2mum and formed by flower balls assembled by nanosheets. The method has the advantages of simple preparation technology, mild condition, short operation period, and convenient realization of batch production, and the prepared film which has a unique space morphology and a good photocatalytic activity can be applied to the photocatalystic degradation of organic pollutants in water or air.

The invention discloses a luminous nano composite fiber film containing rare earth polyacid and a method for preparing the luminous nano composite fiber film, and belongs to the field of inorganic-organic composite materials. The method includes the steps that a polyacrylonitrile solution is prepared, the rare earth polyacid is added to the solution, and the rare earth polyacid / polyacrylonitrile luminous nano composite fiber film is prepared by means of the electrostatic spinning technology. The method achieves the fact that rare earth polyacid functional molecules and water-insoluble polymer polyacrylonitrile form the nano composite fiber film by means of electrostatic spinning for the first time, and expands the application field of the rare earth polyacid and polymer materials. The thickness of the prepared fiber film is controllable in a micron grade, meanwhile the space confinement function in filaments of nano composite fibers is used, and solidification of rare earth polyacid luminescent molecules is achieved. The luminescence intensity of the fiber film can be controlled according to changes of spinning time, and the fiber film is strong in operability. The fiber film can serve as a pH sensitive gas sensing material, and is applied in novel photoelectric equipment.

The invention provides a capillary tube with a fixed phase formed by culturing cells through adhering the cells on the inner wall of the capillary tube, a manufacturing method thereof, and application thereof to medicament screening with a capillary tube electrophoresis method. Specific to the change of native conformation caused by separation and purification of certain biologically active macromolecules from cells, the invention develops the capillary tube electrophoresis method for researching the coaction between biologically active macromolecules and an active ligand or a compound and for screening medicaments by using a fixed phase prepared by culturing cells overexpressed by the biologically active macromolecules in a capillary tube chromatographic column and by fixing after adherent growth of the cells.

The invention discloses a catalyst for degrading chlorophenol pollutants and a preparation method and applications thereof, and belongs to the fields of environment function materials and catalysis. The catalyst is prepared by following steps: at first, reacting aminated reduced graphene oxide with 3,5-dibromo salicylaldehyde in an ethanol system to synthesize 3,5-dibromo salicylaldehyde Schiff base; and then reacting copper acetate monohydrate with 3,5-dibromo salicylaldehyde Schiff base in an ethanol system to synthesize a Schiff base-copper complex. The catalyst can activate chlorophenol pollutants in a potassium persulfate degradation water solution. The method of eliminating chlorophenol pollutants comprises following steps: (a) preparing a catalyst solution from the catalyst and deionized water, and preparing a potassium persulfate solution from potassium persulfate and deionized water; and (b) mixing the catalyst solution, the potassium persulfate solution, and a water solutioncontaining chlorophenol pollutants, adding the mixed solution into deionized water, and carrying out reactions under vibration in the absence of light. The preparation method is simple, the catalyst activates potassium sulfate under mild conditions, and the energy consumption and pollution are low.

The invention relates to a method for preparing photocatalytic foamed ceramics. The method comprises the following steps: (1) treating potassium tetratitanate raw powder; (2) modifying stearic acid; (3) pre-treating polyurethane foam; (4) preparing primary slurry; (5) carrying out slurry coating for the first time; (6) preparing secondary slurry; (7) carrying out slurry coating for the second time; and (8) carrying out sintering, and cooling to obtain the photocatalytic foamed ceramics. Compared with the prior art, the method has the advantages that the solid content of potassium tetratitanate slurry can be 75 percent by means of ball milling, rinsing, modifying of stearic acid and adding of a dispersing agent; a photocatalyst is immobilized due to the foamed ceramics prepared by direct slurry coating on the polyurethane foam, the contact area between particles and organic matters is enlarged due to the three-dimensional reticular structure of the foamed ceramics, and the photocatlysis performance is improved; slurry coating is directly performed on the foamed ceramics, so that the preparation method is simple, does not need secondary sintering, and is short in cycle and low in cost; and phase change happening at high temperature of potassium tetratitanate is solved by using low-temperature glass as an adhesive.

The invention discloses a method for producing 1,5-pentanediamine through immobilized lysine decarboxylase. The method comprises the following steps of connecting a chitin binding domain (ChBD) gene to the N end of a lysine decarboxylase gene (CadA) to construct a fusion protein; fermenting the fusion protein to obtain a crude lysine decarboxylase enzyme solution; adding chitin into the crude enzyme solution to obtain immobilized lysine decarboxylase; finally, enabling the obtained immobilized lysine decarboxylase to participate in a decarboxylation reaction of L-lysine to prepare 1,5-1,5-pentanediamine. According to the method, the specific affinity effect of the substrate chitin and the fusion protein is skillfully utilized to adsorb the lysine decarboxylase, the cost of the immobilizedcarrier chitin is low, the immobilization efficiency is high, the activity stability of enzymes is high, and meanwhile, the procedure of protein purification can be omitted through specific affinity adsorption. The provided method achieves repeated production of 1,5-1,5-pentanediamine and can reduce the production cost, simplify the separation procedure of downstream products and enzymes and achieve remarkable economic benefits.

The invention provides a method for preparing conducting polyaniline immobilized enzyme with a nanometer structure. The method comprises the following steps of: 1, adding aniline monomers serving as a template, or an acid dopant which contains a surface active agent and serves as a soft template, or polystyrene (PS) serving as a hard template into deionized water in which the aniline monomers are dispersed, and at room temperature, magnetically stirring the mixed solution to obtain uniform emulsion; under an action of electrostatic force, adding aqueous solution of oxidant into the emulsion at one time to obtain the conducting polyaniline with the nanometer structure; and 2, dispersing the obtained conducting polyaniline in buffer solution of phosphoric acid, uniformly stirring the mixed solution, dissolving the free enzyme in the same buffer solution, performing covalent coupling of the mixed solution on a shaking table at the speed of 60 to 120rpm, washing the mixed solution and removing supernatant to obtain the immobilized enzyme having the nanometer structure and using the conducting polyaniline as a carrier. The immobilized enzyme prepared by the method can be applied to the decoloring reaction of dyes, and has the advantages of mild reaction conditions, high degradation efficiency and repeated use.

The invention discloses a method for immobilizing human source arginase-1 through surface display. The method comprises the steps of adding signal peptide and charged polypeptide to the amino terminal of a protein cleavage variant (InaK-N) formed on an ice core, fusing human source arginase-1 into the carboxyl terminal, and designing an HA label at the carboxyl terminal; constructing various recombinant plasmids to convert competent escherichia coli cells, so that different genetic engineering strains are obtained; conducting shake-flask culture on the strains; detecting the display efficiency and enzyme activity of human source arginase-1; selecting the strain with the highest enzyme activity for mass culture, conducting efficient L-arginine conversion, and synthesizing L-ornithine. Human source arginase-1 fused in the protein cleavage variant formed on the ice core is effectively displayed on the surface of an escherichia coli cell, so that human source arginase-1 is immobilized. Compared with an original ice core protein display system, the method has the advantage that the display efficiency and enzyme activity of human source arginase-1 are improved remarkably. Compared with a chitin immobilizing method, the method has the advantages that cost is reduced, process is shortened, and purification steps are simplified.

The invention discloses ultrafiltration / adsorption bi-functional film and a preparation method thereof. The preparation method comprises the steps of firstly blending polyether sulfone, polyvinylpyrrolidone, polyethyleneimine and N,N-dimethylformamide so as to obtain film casting liquid, forming a film from the film casting liquid by virtue of an immerse precipitation method, reversely filtering dispersed hollow nano-Fe3O4 turbid liquid from the back of the film so as to fix nano-particles into finger-shaped holes of the film, and finally sealing big pores on the back of the film by virtue of an organic polymer, so as to obtain Fe3O4 encapsulated ultrafiltration / adsorption bi-functional film. According to the preparation method, the ultrafiltration / adsorption bi-functional film which is good in hydrophily, high in flux and retention rate and strong in heavy metal adsorption capacity is prepared by virtue of the immerse precipitation method and the reverse encapsulation of the Fe3O4 nano-particles. A preparation process of the ultrafiltration / adsorption bi-functional film is simple and relatively low in cost and has extremely high application values in the field of treatment of heavy metal ions and macromolecular pollutants.

The invention discloses a heterogeneous ultraviolet-Fenton oxidation catalyst and preparation and application thereof. Prussian blue is used as iron sources, the Prussian blue is deposited on an amino-functional SiO2 mesoporous film with vertical hole passages in an automatic assembly manner, the Prussian blue is fixed through the physical absorption and the interaction force between the positive and negative charges, the bonding is firm, and the loosing is avoided; the prepared catalyst is the film catalyst, the specific surface area is large, the utilization efficiency of active components is high, the catalytic efficiency is high, and compared with the powdery and granular catalysts, the separation and the recovery are easy. Because the catalyst is provided with the mesoporous passages which are vertically distributed, the internal diffusion resistance is little, and many organic matters are suitable for being treated; at the same time, the Prussian blue loaded by the catalyst contains various states of iron, so that the Prussian blue has a cooperating function; in addition, the preparation method disclosed by the invention is simple in technology and wild in condition, so that the preparation method is suitable for popularization and application.

The invention discloses a method for removing phenolic substances in a phenol aqueous solution by using a tyrosinase-metal organic framework compound. The tyrosinase-metal organic framework compound is added into the phenol aqueous solution to react with the phenolic substances so as to achieve the purpose of phenol removal; the tyrosinase-metal organic framework compound is prepared by the following method: mixing metal ions or metal clusters, organic ligands and a tyrosinase solution so that tyrosinase is immobilized on a porous hybrid metal-organic framework generated by the metal ions or the metal clusters and the organic ligands through coordination; the tyrosinase-metal organic framework compound is obtained. The tyrosinase-metal organic framework compound is simple in preparation process, low in cost, environmentally friendly and capable of efficiently removing the phenolic substances in water.

The invention provides a preparation method for synthesizing a polyphenol compound by using an enzyme immobilization technology. The preparation method comprises the following steps: mixing metal ionsor metal clusters with an organic ligand and tyrosinase, and immobilizing the tyrosinase to obtain a tyrosinase-metal organic framework compound; and using the tyrosinase-metal organic framework compound as a catalyst and a monophenol compound as a reactant so as to synthesize the polyphenol compound. A metal organic framework is formed by coordination of the metal ions or the metal clusters andthe organic ligand under the induction of the tyrosinase. Meanwhile, the metal organic framework forms a tyrosinase protective shell, so that the tyrosinase is immobilized, and the obtained immobilized tyrosinase has relatively high catalytic performance. Monophenol compounds are catalyzed by the immobilized tyrosinase to synthesize an ortho-hydroxylated polyphenol compound, and a reducing agent is added to inhibit the polyphenol compound from being further oxidized, so that the yield of the polyphenol compound is increased.

The invention provides a multi-adsorption biodegradable foam with an oil-water separation function, which is characterized in that iron-pillared cationic layered silicate clay is used as an adsorbent, the adsorbent is doped into a sugar-added PBAT phase separation liquid to obtain iron-pillared cationic silicate clay-doped PBAT open-cell foam, and then the surface of the foam is coated with a PAM / SiO2 hydrophilic coating to obtain the super-hydrophilic open-cell foam is obtained. The foam material is hydrophilic and oleophobic, is light in weight, can remove methylene blue, copper ions and other cationic water pollutants in water, has a light degradation reaction due to the existence of iron ions, can be subjected to light degradation after adsorption, has a good cyclic adsorption function, can be repeatedly used, and is biodegradable. And when the adsorption capacity is lost, the foam is easy to degrade, does not pollute the environment and has wide use value.