The invention provides a preparation method of a functional graphene oxide sponge. According to the method, sulfonated calix [4] arene and GO (graphene oxide) are synthesized firstly, sulfonated calix[4] arene and GO are subjected to a hydrothermal reaction, grapheme hydrogel is prepared and subjected to freeze drying, and sulfonated calix [4] arene modified 3D S-GOS (stereoscopic-graphene oxidesponge) is obtained. The material is subjected to characterization analysis with analytical means such as SEM, EDS, FT-IR, XRD and the like. With the adoption of a static adsorption method, influenceof pH value, ion strength, adsorption time, initial uranium concentration and temperature of an initial solution on S-GOS adsorbed uranium (VI) is researched, and adsorption kinetics and adsorption thermodynamics of the S-GOS are studied. The regeneration-recycle performance of the S-GOS adsorbed uranium (VI) is investigated finally. The synthesis method is simple, one new material with large superficial area and high supramolecular recognition and enrichment capacity is obtained, and the material is good in adsorptive property, convenient to recycle and excellent in biocompatibility.