838results about How to "Small amount of sample" patented technology

The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen / antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen / antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.

The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen / antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen / antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.

This invention provides methods, devices and device components for sensing, imaging and characterizing changes in the composition of a probe region. More particularly, the present invention provides methods and devices for detecting changes in the refractive index of a probe region positioned adjacent to a sensing surface, preferably a sensing surface comprising a thin conducting film supporting surface plasmon formation. In addition, the present invention provides methods and device for generating surface plasmons in a probe region and characterizing the composition of the probe region by generating one or more surface plasmon resonances curves and / or surface plasmon resonance images of the probe region.

A rugged, miniature, spectroscopic gas analyzer apparatus for rapid, non-invasive, multi-component breath monitoring and analysis and subsequent determination of Q or other medical diagnostic applications. The system is comprised of one or more IR emitters focussed by optical elements through a low volume sample cell receiving a sample input of a patient's breath for analysis. The patient either at rest or during exercise, inhales C2H2—SF6 mixtures (balance of oxygen and nitrogen) which is subsequently monitored upon exhalation for CO2, H2O, C2H2, and SF6 which can be employed to determine Q directly and accurately. Measurements are performed in real-time or via post-processing of stored original data. Due to its small size, ruggedness, and low power consumption, the monitor can conveniently be employed in the field or data can also be retrieved remotely using telemetry. The miniature analyzer operates on the principle of infrared absorption spectroscopy and allows very precise concentration measurements of the analytes of interest, without any bias or interference from other matrix components.

The invention discloses a cascading isothermal amplification based miRNA fluorescence detection kit and a miRNA fluorescence detection method. The target miRNA can specifically recognize and form a stable three-way crossing structure with a ternary probe through complementary hybridization of nucleic acids; a ternary primer in the three-way crossing structure performs strand displacement amplification (SDA) along a ternary template and generates a number of SDA template products; the SDA template product can open a difunctional hairpin probe to trigger nickase to perform signal amplification reaction finally; and a cyclophorase digestion molecular beacon can generate cascading amplified fluorescence signals. The kit can detect miRNA low to 100fM; and the detection method has superhigh specificity, can well distinguish highly homologous sequences at different mispairing positions and solves the difficult problem of false positive result caused by poor specificity in the existing detection method.

The present invention is related to the field of bio / chemical sampling, sensing, assays and applications. Particularly, the present invention is related to how to make the sampling / sensing / assay become simple to use, fast to results, highly sensitive, easy to use, using tiny sample volume (e.g. 0.5 uL or less), operated by a person without any professionals, reading by mobile-phone, or low cost, or a combination of them.

The invention discloses a method for identifying the aging degree of a silicon rubber composite insulator. The method comprises the following steps: determining the states of umbrella skirt test samples of the silicon rubber composite insulator by taking the operating age limit as reference, and selecting a sample area of each state to obtain an average-value test sample of each state; sampling and cutting into slices; performing microscopic infrared scanning on the slices by taking silicon methyl 1296 cm<-1> as a calibrated peak to obtain microscopic infrared micro-area scanning images of the slices with different age limits; calculating the ageing depth H value from the microscopic infrared micro-area scanning images, fitting the relationship between the H value of the samples with different age limits and time to obtain an ageing model; quantitatively identifying the ageing degree of an unknown sample according to the ageing model. According to the method, the ageing degree of the silicon rubber composite insulator can be quantitatively analyzed by a microscopic infrared Mapping scanning technology; the change of the silicon rubber composite insulator from the surface to the center along with ageing can be observed; the visualization degree is high.

The invention discloses a spinal muscular atrophy related gene copy number detecting kit and method based on a gene trapping and second-generation sequencing technique. The kit comprises capture probes, wherein the capture probes include four main probes and related control probes. The four main probes are the probe for capturing an SMN2 gene exon 7 and shown as SEQ ID NO:1, the probe for capturing an SMN2 gene exon 7 and shown as SEQ ID NO:2 the probe for capturing an SMN1 gene exon 7 and shown as SEQ ID NO:3 and the probe for capturing an SMN1 gene exon 7 and shown as SEQ ID NO:4, the capture probes conduct capture and sequencing on a target area, and the copy number of SMN1 and SMN2 gene exons 7 is further estimated through the data analysis method. The problem that an existing method is not uniform in sequencing depth, higher in error rate, poor in stability, smaller in flux and the like is solved.

A sample-carrier complex (119) is introduced into a sample introducing portion (107), and the sample-carrier complex (119) is moved and deposited on a damming portion (111). The damming portion (111) is heated at a stage in which the predetermined amount of sample-carrier complex (119) is deposited on the damming portion (111). A temperature is increased to a predetermined temperature to break down the sample-carrier complex (119) into a sample (121) and a carrier (123). A voltage is applied between the sample introducing portion (107) and a sample recovery portion (109) to cause the sample (121) to pass through a gap between columnar bodies (115) and move into a second channel (106) to perform predetermined separation and analysis or recovery operation.

A method for measuring particle diameter distribution includes placing particle samples in glass sample tank set on back light plate uniformly with particle sample number of 50 - 500, placing size calibration object in the tank, using digital camera to shoot image of said particle sample, guiding image to computer for processing the image and obtaining roundness and diameter of each particle, outputting particle sample distribution results of number, volume and weight according to division of particle diameter.

The invention provides a test method for trypsin. The test method comprises the following steps that: the fluorescence intensity of a metal nanocluster is tested; the trypsin reacts with the metal nanocluster, so that a reaction product is obtained, and the fluorescence intensity of the reaction product is tested; the difference between the fluorescence intensity of the metal nanocluster and the fluorescence intensity of the reaction product is calculated, and according to the difference and a predetermined standard curve, the content of the trypsin is obtained. The test method provided by the invention adopts the metal nanocluster as a fluorescence probe, the metal nanocluster comprises protein and metal, the property of the trypsin in fluorescently quenching the metal nanocluster is utilized, moreover, the degree of fluorescence quenching is positively correlated to the concentration of the trypsin, and the fluorospectrophotometry is adopted to determine the content of the trypsin. The test method provided by the invention has the advantages of high accuracy, high sensitivity and high selectivity, and an experimental result shows that the sensitivity of the test method provided by the invention on the test of the trypsin is 1.0 multiplied by 10 negative 9g / mL.

The invention discloses a capillary needle. The capillary needle comprises a tube body, the two ends of the tube body are respectively provided with an inlet and an outlet for electro-spray, a part of the tube body is bent to form a sampling end, and the sampling end is provided with a sampling port communicating with a cavity in the tube body. The invention also discloses an electro-spray ionization mass spectrometry analytical apparatus applying the capillary needle. The invention also simultaneously discloses a method applying the electro-spray ionization mass spectrometry analytical apparatus for analytical detection. The analytical apparatus is high in integration, compact in structure, easy to control, small in size and low in cost; the sample consumption is greatly reduced, and the experiment cost is decreased; an integrated sample introduction method is employed, and an sample introduction end does not have to be repeatedly placed in a sample and blank solution, such that cross contamination is reduced, and more importantly, the analytical flux is substantially improved; and through combination with an automatic sample changing system, different samples can be automatically introduced, and therefore, the capillary needle, the apparatus and the method are quite suitable for such fields as high-flux screening, unicell analysis, mass spectrum imaging analysis and the like.

The invention discloses a kinetic analysis device of gas hydrate. The kinetic analysis device consists of a pressure-stabilized gas supply unit, a gas hydrate generation / decomposition unit, a kinetic analysis unit, a temperature control unit and a data acquisition and processing unit; moreover, the device carries out research on the generation / decomposition process of gas hydrate by means of resistance, torque, and the like, and refits gas phase chromatogram, thereby reducing sampling quantity of gas phase samples and avoiding variation in each phase caused by component reduction. The kinetic analysis device realizes kinetic analysis during gas hydrate generation / decomposition and ensures comprehensive, actual and accurate kinetic data result by means of resistance, torque, blender rotating speed and gas phase chromatogram, and the like. The device can be used in kinetic analysis of mixed gas hydrate generation / decomposition and kinetic analysis and experimental research of gas hydrate added with chemical reagents. Moreover, the method and the device are simple and have convenient operation.

The invention discloses a method for quickly testing the appropriate granulation moisture content of an iron ore sinter mixture. The appropriate granulation moisture content refers to the moisture content required by the granulated mixture for obtaining optimal breathability. The moisture includes the moisture of the raw materials and the added water during mixing and granulation. The method comprises the following steps: 1) preparing a slurry mixture of which the humidity is 10 to 20 percent from iron ore, solvent, coke powder and sinter return ore; 2) rinsing and sieving the mixture by using saturated lime water as a medium, obtaining adhesive powder and shell grains and testing the content Xa of the adhesive powder; 3) testing the inner hole water-holding capacity of the shell grains by centrifugation; 4) testing the saturated water-holding capacity of the adhesive poser by using saturated water absorption process; and 5) calculating the appropriate moisture content required for the granulation of the mixture according to a formula. The method has the characteristics of simple operation and high accuracy. Based on an absolute error range from -0.3 to +0.3, the accuracy of the prediction of the appropriate moisture content reaches 93.3 percent according to the results of the tests of the moisture content of mixtures prepared by 30 material mixing schemes.

The present invention provides nano particle size measuring method and device with scattered dynamic low-strength laser. The method includes: radiating liquid sample with monochromic laser beam of proper length to produce scattered light signal; collecting and transmitting to light signal with single-modular fiber with gradient refractive index; recording with single-photon counter module the scattered photons and converting into output electric pulse signal; and processing the signal in an autocorrelator. The device includes: light source, polarizer, focusing lens, two-layered refractive index sample matching pool, fiber, single-photon counter module, autocorrelator and computer. The present invention can measure nano particle size fast and accurately.

The invention relates to detection technology for element contents, and provides a method for simultaneously determining contents of arsenic, cadmium, lead, vanadium, zinc, manganese and phosphor in soil or deposits. The method mainly comprises the following steps: (1) 0.1g of a grinded drying soil sample is accurately weighed and placed in a TFM microwave digestion pot, a certain proportion of aqua regia and hydrofluoric acid are added, and a solution is allowed to stand; (2) an appropriate microwave digestion program is set, and microwave digestion is carried out for the solution in the step (1); (3) after the microwave digestion program ends, an acid-driving device is used for heating and evaporating the digestion solution in the step (2) to a dry state, and total transfer, volumetric flask preparation, and filtering are carried out; (4) ICP-OES is used for determining contents of arsenic, cadmium, lead, vanadium, zinc, manganese and phosphor in the clear liquor in the step (3); (5) ICP-MS is used for determining contents of elements in the clear liquor in the step (3) which do not reach detection limits in the step (4) once more. The method has the advantages of simple steps, simplified operation, saved soil samples, reduced personal error, minimized environment interference, and improved working environments.

The invention discloses an immunochromatography kit for fluorescence quantitative detection of salbutamol and a preparation method of a fluorescence labeling liquid. The kit comprises a test strip and a fluorescence labeling liquid, wherein the test strip is formed by sequentially lapping and sticking a sample pad, a nitrocellulose coating film and absorbent paper on a bottom plate; the nitrocellulose coating film comprises a detection area and a quality control area; the detection area is coated with an SAL-BSA conjugate; the quality control area is coated with anti-rabbit IgG; and the fluorescence labeling liquid contains a fluorescence labeling SAL antibody and a fluorescence labeling rabbit IgG. Compared with an immune colloidal gold labeling test strip, the kit disclosed by the invention has the advantages of higher sensitivity, accurate quantification and the like; and the operation is faster and more convenient than that of an enzyme coupling method.

The invention discloses a method for quickly evaluating the taste of bayberry juice through an electronic tongue system. The method comprises the steps of constructing a bayberry juice taste scoring and electronic tongue response signal relevance model, verifying the bayberry juice taste scoring and electronic tongue response signal relevance model, acquiring taste fingerprint information of a bayberry juice sample to be detected through the electronic tongue system, and calling the taste fingerprint information into the verified bayberry juice taste scoring and electronic tongue response signal relevance model to obtain a taste evaluation forecast value of the bayberry juice sample. The method for quickly evaluating the taste of the bayberry juice through the electronic tongue system is high in forecast precision and easy to operate and cannot be affected by external factors; compared with the conventional taste evaluation method, the method disclosed by the invention is high in reproducibility, objective, accurate and time-saving and is a new method for evaluating the taste of the bayberry juice.

This invention discloses one liquid phase chip test agent case and its process method for multiple kinds of anti-EB virus antibody, which comprises the following parts: coupling affinity element multi-color microball covering on polypeptide; at least one kind of coupling hydroxyl group multi-color microball covering on natural or gene antigen, wherein the antigen is EB virus crack object or shell antigen VCA; gene antigen is of one amino acid in list of No. 33-No. 37; the said integration polypeptide is one from No. 1-No. 32.

The invention discloses a method for quickly detecting copper ions in a solution. The method comprises the following steps of: reacting a reducible water-soluble compound with a water-soluble compound which contains precious metal in a polyhydroxy water-soluble compound which is used as a protective agent to obtain a detecting solution containing precious metal nano particles, which are modified by the polyhydroxy water-soluble compound; supplying two parts of the same detecting solutions with equal quantities, adding the solution without the copper ions and a solution to be detected, which are isometric, into the two parts of the detecting solutions respectively, and forming a first mixing solution and a second mixing solution which are corresponding; and judging whether the copper ions exist in the solution to be detected or not according to the color variation of the second mixing solution relative to the color of the first mixing solution or according to the variation of the ultraviolet visible adsorption strength or peak position of the second mixing solution relative to that of the first mixing solution, wherein the copper ions are divalent copper ions. The method disclosed by the invention is simple and is flexible in design, free from a large-scale instrument, high in sensitivity and selectivity, strong in stability, good in reproducibility of the detecting result, and low in cost.

The invention provides a preparation method of a multifunctional surface enhanced raman scattering (SERS) substrate. The preparation method comprises the following steps of: firstly, paving a layer of ZnO seeds on the inner wall of a capillary, developing a ZnO nanorod array in Zn(NO3)2 and hexamethylenetetramine (HMT) solution, and soaking and reacting the capillary in solution of (NH4)2TiF6 and H3BO3, thus obtaining a TiO2 nanotube array; and soaking a nanotube in which TiO2 is grown into SnCl2 to ensure that a layer of bivalent tin ions are adsorbed on the surface layer of the nanotube, soaking the nanotube into Ag(NH3)2+ and reducing the nanotube to silver particles, thus obtaining the multifunctional SERS substrate. Compared with the conventional SERS substrate, the multifunctional SERS substrate based on the capillary has functions of raman enhancement and repeated use, is convenient to carry and sample, and can realize real-time field detection.

The invention discloses a method for detecting a metal cation by utilizing a VA group or VIA group element compound. The VA group or VIA group element compound is utilized and interacts with the detected metal cation to form a core-shell structure on the surface of gold nanoparticles, wherein the gold nanoparticles are taken as the core, and the compound formed by interaction of the VA group or VIA group element compound and the detected metal cation is taken as the shell. The resonance absorption peak of the surface plasma of the gold nanoparticles is changed so that the color of the solution is changed, thus the change of the color of the solution can be directly distinguished by naked eyes or simple instruments and equipment to realize the purpose of quickly detecting the detected metal cation in the solution system. The method has the advantages of simple and convenient operation, low detection cost, high detection sensitivity, small sampling quantity and wide application range.

The invention discloses a method for qualitative detection of Raman spectra of compositions of blend fabrics. The method is to directly detect and take the laser Raman spectra of the fabric, combine the spectrum pretreatment technology and the method for extracting, identifying and analyzing characteristic peaks, and qualitatively identify the composition sources of the fabrics. The detection method belongs to a pure optical method, needs low sample amount, does not need pretreatment, has short test time, undamaged samples during the test process and accurate test results, does not generate chemical pollutants and is suitable for qualitative detection of the compositions of various blend fabrics.

The invention relates to a method of quantitatively detecting metal ions and / or small-molecule compounds through nuclear magnetic resonance. The method includes steps of 1) providing a liquid to be detected and a detection primary liquid, and 2) adding the liquid to be detected into the detection primary liquid, measuring the nuclear magnetic resonance transverse relaxation time of the liquid mixture, and acquiring the content of the metal ions and / or small-molecule compounds in the liquid to be detected according to the measured nuclear magnetic resonance transverse relaxation time. The method has advantages of simple operation, rapidness, a low cost, good selectivity, high sensitivity and capability of on-site real-time operation. The method is suitable for lake and river water quality survey, sewage discharging port water quality detection, and detection of melt mineral compounds, household water and various water samples or ethanol samples after treatment, and can be widely applied for the fields of food science, biomedicine, environmental science, analysis science, and the like.

The invention provides a testing method and a kit for secondary circulation amplification of microRNA (Ribose Nucleic Acid). According to the detection method provided by the invention, a secondary circulation amplification reaction can be finished by only a single operation, the sensitivity is high, and nine RNA chains in a 15-microliter reaction system can be detected; the design is simple, the compounding and modifying of nanometer materials are not related; the noise is low, so that the sensitivity, specificity and generality are increased; compared with a conventional Northern blot assay method and a conventional microarray assay method, the required sample amount is little, and the specificity is excellent; compared with a real-time quantitative PRC (Polymerase Chain Reaction), the method is a thermostatic reaction, so that the test can be realized on a normal fluorescence instrument, the use range of the method is enlarged, and the sequence design is simple and convenient; and the miRNA in a level of a single breast cancer cell can be detected. The technique disclosed by the invention can be used for designing diagnostic kits and devices for tumor.

The invention discloses a dopamine derivative, a molecular imprinted polymer and preparation methods and application thereof. The dopamine derivative comprises dansyl dopamine, carbazole sulfonyl dopamine and the like. The dopamine derivative is synthesized from a fluorescent color former precursor and dopamine through sulfonation reaction. The fluorescent molecular imprinted polymer is prepared by using the fluorescent dopamine derivative, integrates specific sample pretreatment and fluorescence detection and is linked with an ELISA (Enzyme-Linked Immuno Sorbent Assay) plate, so as to form a fluorescent sensor, and series acidic organic pollutants, including bisphenol A and 2,4-dichlorophenoxyacetic acid, in an environmental water sample are directly assayed according to the change of fluorescence quenching values before / after sample application.

The invention relates to the field of textile materials, and aims at providing a method for identifying a bamboo hemp fiber by using a terahertz time-domain spectroscopy technique. The method comprises the following steps of: respectively mixing and pressing a fiber with polyethylene powder to prepare a disc sheet after preparing the powder from the fiber as a guide sample; testing by adopting a transmission-type terahertz time-domain spectroscopy test device, thus respectively obtaining terahertz pulse time-domain waveforms through each guide sample; calculating the absorption coefficient and the refractive index of each guide sample, so as to respectively draw out an absorption spectrum and a refraction spectrum of the fiber; obtaining an absorption coefficient and a refractive index of the fiber to be identified; and confirming the type of the fiber to be identified according to the corresponding relation with the absorption spectrum and the refraction spectrum of each guide sample. According to the method, required sample quantity is simple, samples are simple and convenient to prepare, test time is short, and chemical pollutants are not caused in a test process; the absorption spectrum and the refraction spectrum of the sample at a terahertz waveband can be obtained at the same time when the sample is tested; the feature information of the samples is increased; the reliability of a test result is also improved.

The invention discloses a method for analyzing a hydrogen isotope in water of fluid inclusion. According to the method, a water sampling instrument and a TC / EA (Temperature Conversion / Elemental Analyzer)-IRMS device are utilized; the water sampling instrument comprises a vacuum communication pipeline, a sample cracking device and a water sample collecting device, the water sample collecting device comprises a quartz collecting tube, and a capillary tube part is formed at the lower end of the quartz collecting tube; the TC / EA-IRMS device comprises a TC / EA and an IRMS, and the TC / EA comprises a high-temperature reaction tube and a gas-phase chromatographic column. With the capillary tube part and a solid feeding manner, the quantity of required samples is small, and thus the quantity of samples of the fluid inclusion can be effectively reduced.

The invention relates to a method for measuring polyvinyl chloride content in plastic through thermal cracking-gas chromatography mass spectrometry. The method comprises four steps of sample pretreatment, thermal cracking-gas chromatography mass spectrometry measurement, qualitative analysis and quantitative analysis, wherein the steps are specifically are as follows: extracting polyvinyl chloride in a plastic sample through tetrahydrofuran oscillation, cracking the polyvinyl chloride through a thermal cracking device, analyzing the crackate through a gas chromatography mass spectrometry instrument, analyzing the characteristic crackate of polyvinyl chloride to determine the nature so as to judge whether the sample contains polyvinyl chloride, and finally, quantifying the characteristic crackate through a benzene external standard method so as to measure the polyvinyl chloride content in the sample. The method provided by the invention can be used for accurately quantifying the polyvinyl chloride content in plastic, the method is simple in pretreatment, few in sampling, fast in test and good in specificity, and the detection limit of the method is 1000 mg / kg.

The invention provides a primer, a probe and a kit for detecting rotavirus and Norovirus liquid phase chips. The primer comprises three pairs of primers of a specific amplification A type rotavirus sequence, a GI type Norovirus sequence and a GII type Norovirus sequence. The probe comprises three specificity detection probes of A type rotavirus, GI type Norovirus and GII type Norovirus. The kit comprises the three pair of primers and a specificity detection microsphere mixture prepared by coupling the three specificity detection probes and a fluorescence coding microsphere. The experiment proves that the primer and the probe disclosed by the invention are characterized in that a method of combining multiple PCR (polymerase chain reaction) with liquid phase chip detection can simultaneously and accurately detect the A type rotavirus, the GI type Norovirus and the GII type Norovirus. The primer, the probe and the kit have the advantages of strong specificity and high sensitivity.