Capillary needle, and electro-spray ionization mass spectrometry analytical apparatus and method

An electrospray mass spectrometer and analysis device technology, applied in the field of mass spectrometry, can solve the problems of cumbersome sample replacement, limitation of high-throughput screening applications, and difficulty in realizing multi-sample detection, etc., achieve small size, reduce sample consumption, and save experimental costs Effect

Active Publication Date: 2015-03-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the device can only be used for the generation and detection of droplets with the same chemical composition, the sample replacement is cumbersome, and it is difficult to realize the detection of multiple samples, which limits its application in high-throughput screening.

Method used

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  • Capillary needle, and electro-spray ionization mass spectrometry analytical apparatus and method
  • Capillary needle, and electro-spray ionization mass spectrometry analytical apparatus and method
  • Capillary needle, and electro-spray ionization mass spectrometry analytical apparatus and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] figure 1 It is a schematic diagram of an electrospray mass spectrometry device for sampling sub-oil droplet samples with micro-volume and high-throughput sample sampling capabilities established according to the present invention. The device consists of a capillary spray needle 10 integrated with an injection port 1, a sampling port 2, a sampling port 3, and an outlet 4, a set of liquid driving device 5, a set of movable sample replacement system 6, and a set of high-voltage power supply 7 , and a mass spectrometer 8. figure 1 The middle arrow indicates the direction of buffer movement.

[0044] The capillary needle 10 is processed by a capillary tube, including a tube body. The two ends of the tube body are respectively the injection port 1 and the outlet port 2 of the buffer solution. A part of the tube body is bent in a U shape to form a sampling end 2. The sampling end is provided with a The inner cavity of the tube communicates with the sampling port 3 . The sam...

Embodiment 2

[0052] Figure 4 It is a sampling analysis device and a method of use thereof according to the preferred embodiment 1 of the present invention. Taking choline as a sample, by detecting the mass spectrometry signals of the under-oil droplets with different choline concentrations, the concentration of choline in the under-oil droplets and Standard curve for the relationship between mass spectrometric signal intensities.

[0053] Specifically, the concentrations of choline are respectively 0.2mM (mmol / L), 0.4mM, 1.0mM, 2.5mM, 5.0mM, 8mM and 10.0mM, and the volume of the droplets is 300 nanoliters. The number is 8. Obtain the standard curve I=3632C+1694(R 2 = 0.999). According to the standard curve, the choline concentration in different droplets can be calculated by mass spectrometry signals.

[0054] Figure 5 is to utilize the analytical device of preferred embodiment 1, and Figure 4 The enzyme inhibition curves of the three effective inhibitors of AchE were obtained fro...

Embodiment 3

[0058] figure 2 It is a schematic diagram of the analysis device of Example 3. Using a capillary sampling device similar to that of Example 1, the sample 9 solution in the porous plate 14 was sampled and analyzed. The processing method of the capillary in this embodiment is the same as that in Embodiment 1. The multiwell plate 14 is a commercially available 96-well plate.

[0059]The specific usage method of the analysis device described in Example 3 is as follows: (1) start the liquid drive device 5, continuously inject the electrospray buffer solution from the buffer solution injection port 1 of the capillary needle 10, and pass through the sampling port 2 and the sampling port 3, Reach the electrospray nozzle outlet 4, and start the high-voltage power supply 7, so that the buffer solution is ejected from the electrospray nozzle outlet 4 to form a stable electrospray; (2) the sample 9 solution to be analyzed is stored in the porous plate 14; (3 ) The porous plate 14 is p...

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Abstract

The invention discloses a capillary needle. The capillary needle comprises a tube body, the two ends of the tube body are respectively provided with an inlet and an outlet for electro-spray, a part of the tube body is bent to form a sampling end, and the sampling end is provided with a sampling port communicating with a cavity in the tube body. The invention also discloses an electro-spray ionization mass spectrometry analytical apparatus applying the capillary needle. The invention also simultaneously discloses a method applying the electro-spray ionization mass spectrometry analytical apparatus for analytical detection. The analytical apparatus is high in integration, compact in structure, easy to control, small in size and low in cost; the sample consumption is greatly reduced, and the experiment cost is decreased; an integrated sample introduction method is employed, and an sample introduction end does not have to be repeatedly placed in a sample and blank solution, such that cross contamination is reduced, and more importantly, the analytical flux is substantially improved; and through combination with an automatic sample changing system, different samples can be automatically introduced, and therefore, the capillary needle, the apparatus and the method are quite suitable for such fields as high-flux screening, unicell analysis, mass spectrum imaging analysis and the like.

Description

technical field [0001] The invention belongs to the field of mass spectrometry, and in particular relates to a capillary needle, an electrospray mass spectrometry device with the capillary needle and a method for mass spectrometry using the device. Background technique [0002] Using electrospray mass spectrometry detection technology, various sample molecules can be directly detected without labeling, and the detection results with rich information can be obtained. Compared with optical detection methods that need to embed fluorescent or chromogenic groups in sample molecules, electrospray mass spectrometry detection will not change the original biological activity of molecules, and can avoid false positive / negative results, thereby improving research efficiency. Reduce research costs. However, the existing electrospray mass spectrometry method not only consumes samples in the range of microliters to milliliters, but also has a much lower analytical throughput than optical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01J49/04H01J49/26G01N27/62
CPCH01J49/04H01J49/26G01N27/62
Inventor 金迪琼方群祝莹
Owner ZHEJIANG UNIV
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