The invention discloses an integrated chip system for high-throughput sorting and counting detection of biological particles, and an application. The chip system comprises a main microfluidic chip, a micropipe, a sample liquid micropump, an exchange liquid micropump, a first waste liquid collecting device, a second waste liquid collecting device, a third waste liquid collecting device, laser emitters, photoelectric conversion devices, optical fibers and a computer, wherein the main microfluidic chip comprises an asymmetric curved flow path, a first branch channel, a second branch channel, a third branch channel, a main flow path, a branch flow path, aligning marks, etc. The system utilizes the asymmetric curved flow path to realize pre-focusing and sorting for the particles, utilizes a liquid changing flow channel to realize change of a carrier liquid of to-be-tested particles and particle cleaning, and utilizes a viscoelastic effect and an inertial effect of a viscoelastic fluid to realize focus of single equilibrium position of section centers of the particles. The system does not need a sheath liquid, complex pre-cleaning of the particles, and optical alignment, has advantages of high speed, high precision, integration, miniaturization, automation, low cost, simple production process, easy batch production, etc.