Integrated chip system for high-throughput sorting and counting detection of biological particles, and application

Abstract

The invention discloses an integrated chip system for high-throughput sorting and counting detection of biological particles, and an application. The chip system comprises a main microfluidic chip, a micropipe, a sample liquid micropump, an exchange liquid micropump, a first waste liquid collecting device, a second waste liquid collecting device, a third waste liquid collecting device, laser emitters, photoelectric conversion devices, optical fibers and a computer, wherein the main microfluidic chip comprises an asymmetric curved flow path, a first branch channel, a second branch channel, a third branch channel, a main flow path, a branch flow path, aligning marks, etc. The system utilizes the asymmetric curved flow path to realize pre-focusing and sorting for the particles, utilizes a liquid changing flow channel to realize change of a carrier liquid of to-be-tested particles and particle cleaning, and utilizes a viscoelastic effect and an inertial effect of a viscoelastic fluid to realize focus of single equilibrium position of section centers of the particles. The system does not need a sheath liquid, complex pre-cleaning of the particles, and optical alignment, has advantages of high speed, high precision, integration, miniaturization, automation, low cost, simple production process, easy batch production, etc.

Description

technical field [0001] The invention relates to the field of micro-flow manipulation and detection, in particular to an integrated chip system for high-throughput sorting and counting detection of biological particles and its application. The chip system mainly includes multi-channel fluid control module, optical detection module, signal processing module and so on. Background technique [0002] Microfluidic technology is a microchip technology that integrates complex laboratory functions and realizes sample preparation, reaction, focusing, sorting, and detection by manipulating microvolume fluids. The sorting and counting of micron-sized biological particles based on microfluidic technology is an important research content in the fields of biomedicine, environmental monitoring, and analytical chemistry. Efficient particle pre-focusing and size purification technology is the prerequisite for high-precision counting. Common focusing and sorting technologies can be mainly div...

AI Technical Summary

Problems solved by technology

Obviously, the traditional artificial technology can no longer meet the high-efficiency sample processing needs of modern laboratories in terms of precision, intelligence, and miniaturization, while the Coulter technology has problems such as easy damage to the activity of biological particles and low detection accuracy.

Based on the optical detection technology of two-dimensional focusing, due to the certain overlapping probability of particles in the third dimension, there is a large error in the counting results

Recent studies have used two sheath fluids to achieve three-dimensional focusing of particles to avoid particle overlap, but the operation of using two sheath fluid entrainment is more complicated

At the same time, due to the low flow rate of most microfluidic chips (the Reynolds number is generally 10 -6 ~10 1 ) and limited by the counting and detection equipment, the throughput of the existing microfluidic technology-based counting and detection of biological particles is very low (generally several to hundreds per second)

In addition, before counting and detecting biological particles, it is often necessary to perform complex pretreatment operations such as filtration, centrifugation, and cleaning on the sample, which requires complex equipment, large volume, and cumbersome operations.

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