436 results about "Semen" patented technology

The invention provides PDRN as well as a preparation method and application thereof, and relates to the technical field of biology. And the GC content of the PDRN is 40.5%-42%. According to the present invention, the similarity between the GC% in the gDNA in the PDRN prepared by using the recorded method and the GC% of the human body is more than 98%, the GC% is closer to the GC% of the human body than the PDRN derived from the salmon, the safety and the efficiency are high, and the PDRN preparation method is simple and convenient compared with the PDRN extracted from the salmon semen in the prior art.

The present application relates to the field of biotechnology. Provided are a PDRN, and a preparation method therefor and the use thereof. The PDRN has a GC content of 39%-42%. In the PDRN prepared by means of the method recited in the present application, the GC% in the gDNA thereof has a similarity of 98% or more to the GC% of human and is closer to the GC% in a human body than that of a salmon-derived PDRN. The PDRN of the present application has higher safety and efficacy, and compared with the extracting of PDRN from salmon semen in the prior art, the method for preparing the PDRN disclosed in the present application is simpler and more convenient.

The invention discloses a method for obtaining high-quality fertilized eggs of wild sal-family pseudosciaena crocea, and belongs to the technical field of aquatic animal breeding. The method comprises the following steps: capturing wild parent fishes in the breeding season of the sal-family pseudosciaena crocea and transporting the wild parent fishes to a farm; the method comprises the following steps: carrying out temporary culture and intensive culture on wild parent fishes, selecting male and female wild parent fishes with mature gonads, anesthetizing, and injecting artificial spawning induction mixed hormones; after artificial spawning mixed hormones are injected, ova and seminal fluid are collected, and high-quality fertilized ova are obtained through artificial insemination. According to the method, all the technical steps are associated with one another to form a whole, the high-quality fertilized eggs of the large yellow croakers of the wild sal ammoniac family can be successfully obtained, and the proportion of the obtained high-quality fertilized eggs reaches 45% or above. According to the method, the obtained high-quality fertilized eggs are adopted for fry hatching, the hatching rate of the obtained fertilized eggs reaches 62% or above, the fertilized eggs can be used for constructing and breeding a wild sal-family pseudosciaena crocea basic population, and artificial breeding of the sal-family pseudosciaena crocea is achieved.

The invention provides an artificial breeding method of black head fish, and belongs to the technical field of aquaculture. The spawning induction method comprises the following steps: injecting spawning induction hormone into a female parent for breeding parent fish twice for artificial spawning induction to obtain fertilized eggs; during the incubation period of the fertilized eggs, micro-flow at 18-21 DEG C is adopted for artificial incubation, and the flow speed is controlled by water at different incubation stages until incubation and seedling emergence are achieved, so that the fry of the black head fish with the odd amount is obtained. According to the method, the oxytocic hormone is injected in a two-needle injection mode, the injection dosage of each needle is strictly controlled, ova and seminal fluid which are well developed can be obtained, meanwhile, the hatching conditions of the fertilized ova are controlled, the oxytocic rate of the black head fish is 100%, the fertilization rate is 89% or above, the hatching rate is 86% or above, large-scale artificial breeding of the black head fish is achieved, and the method is suitable for large-scale artificial breeding of the black head fish. The method has important significance for protecting the cyprinus carpio which is an endangered resource.

The invention provides a breeding method of golden and tiger hybrid spotted fish eggs. The breeding method comprises the following steps: S1, parent screening; s2, parent cultivation; s3, artificial insemination: (1) spawning induction: when the belly of the female parent fish expands, the ovary develops to the IV stage and the male parent fish presses the belly to cause white seminal fluid to flow out, performing oxygenation flowing water stimulation on the female parent fish, and performing irradiation with a TDP radiation lamp for spawning induction; (2) fertilization; s4, artificial incubation: (1) arranging an incubator, injecting seawater, and performing plasma treatment to obtain a treated incubator; and (2) adding the fertilized eggs into the treated incubator, carrying out magnetization treatment, adding chitosan, and hatching to obtain the larva fish. According to the breeding method, the sperm and egg quality can be improved, the fertilization rate and the hatching rate are remarkably increased, and according to verification of the embodiment, the fertilization rate ranges from 97.4% to 99.3%, and the hatching rate ranges from 95.6% to 97.1%. The hatched larvae are not deformed, the survival rate is 96%-99%, the quality of the larvae is high, and a good foundation is established for obtaining high-quality hybrid Jinhuasu.

The invention discloses an artificial breeding method of epinephelus fuscoguttatus and pink pterus coerulescens hybrid species, which comprises the following steps: screening healthy epinephelus fuscoguttatus as a female parent, screening healthy pink pterus coerulescens as a male parent, and carrying out enhanced breeding on the male parent and the female parent, the gonad maturation of the parent is promoted; the method comprises the following steps: collecting egg liquid of a female parent and semen of a male parent, inseminating to obtain a fertilized egg, incubating the fertilized egg to obtain a hybrid spot, performing authenticity identification on the hybrid spot by using one or more of sequences SEQ ID NO.1-SEQ ID NO.8, and determining that the hybrid spot inherits genetic information of the female parent and the male parent. The technical blank of crossbreeding of the pink pink bass as the parent grouper is filled, so that the hybrid obtained by hybridizing the epinephelus fuscoguttatus and the pink pink bass is reliable in authenticity, and inherits genetic information of male and female parents on the molecular level.

The invention relates to an antidepressant pharmaceutical composition and application thereof, and particularly discloses a pharmaceutical composition for treating depression as well as a preparation method and application thereof. The active pharmaceutical ingredients of the pharmaceutical composition are prepared from the following traditional Chinese medicines in parts by weight: 500 to 600 parts of radix bupleuri, 500 to 600 parts of fructus aurantii immaturus, 500 to 600 parts of radix aucklandiae, 500 to 600 parts of pericarpium citri reticulatae, 500 to 600 parts of rhizoma pinelliae preparata, 750 to 900 parts of herba taraxaci, 500 to 600 parts of scorched fructus crataegi, 300 to 360 parts of scorched semen arecae, 500 to 600 parts of paederia scandens, 300 to 400 parts of radix codonopsis, 300 to 400 parts of rhizoma corydalis and 500 to 600 parts of fried medicated leaven. The pharmaceutical composition has a remarkable curative effect on depression, animal experiments show that the pharmaceutical composition can remarkably enhance the social coefficient (showing that the pharmaceutical composition can improve social fear of depression patients) and improve sweet water preference (showing that the pharmaceutical composition is beneficial to transformation of negative emotions such as interest deficiency of the depression patients), and the pharmaceutical composition is safe to take, free of adverse reaction and toxic and side effects and free of toxic and side effects. And the composition can take effect quickly, and can take effect generally within 1-2 weeks after medication.

The invention provides a feed domesticated siniperca chuatsi sperm low-temperature preservation and ultralow-temperature preservation method, which comprises the following steps: firstly, providing a siniperca chuatsi sperm low-temperature preservation diluent which comprises Hanks liquid, sodium chloride, soya bean lecithin, vitamin E, curcumin, melatonin and SOD (superoxide dismutase); and adding an anti-freezing protective agent into the low-temperature preservation diluent to obtain the ultralow-temperature preservation anti-freezing liquid for the siniperca chuatsi sperms. The siniperca chuatsi semen and the low-temperature preservation diluent are uniformly mixed, so that low-temperature preservation can be realized; the siniperca chuatsi semen is uniformly mixed with the cryopreservation anti-freezing liquid, so that cryopreservation can be realized. The siniperca chuatsi sperm low-temperature preservation diluent is preferably selected, low-temperature preservation of the siniperca chuatsi sperms can be achieved, ultralow-temperature preservation can be conducted after an anti-freezing protective agent is added, the persistence of the preservation time of the siniperca chuatsi sperms can be guaranteed, the protection effect on the sperms is good, and the activation rate and activity of the unfrozen sperms are high.

The invention discloses a method for inducing ostrea rivularis triploid, and relates to the technical field of shellfish genetic breeding. The specific preparation method comprises the following steps: firstly, obtaining egg liquid and seminal fluid from gonads of female and male ostrea rivularis individuals, cleaning and curing the egg liquid, and adding the seminal fluid into the egg liquid after cleaning to obtain a fertilized egg solution; when the proportion of the first polar bodies in the eggs of the fertilized egg solution is 30-50%, adding an inducer into the fertilized egg solution for induction and cleaning to obtain induced fertilized eggs; and finally, hatching and screening the induced fertilized eggs to obtain D-shaped larvae, and culturing to obtain the crassostrea rivularis triploid population. The method for inducing the crassostrea rivularis triploid is high in triploid induction rate, high in final hatching rate, capable of stably surviving and good in application prospect.

The invention discloses an artificial cultivation method of a hybrid of epinephelus trifoliatus and epinephelus lanceolatus, which comprises the following steps: taking healthy epinephelus trifoliatus as female parent fish, and carrying out cultivation strengthening and artificial ripening to obtain strengthened female parent fish; healthy epinephelus lanceolatus is used as male parent fish, and sexual mature male parent fish is obtained through natural sexual maturity; the ovulation time and the spermiation time are consistent; collecting egg liquid and seminal fluid, inseminating to obtain fertilized eggs, and performing embryo incubation and illumination regulation and control cultivation after opening to obtain hybrid spots; carrying out authenticity identification on the hybrid spots by utilizing a primer pair, and determining that the hybrid spots inherit genetic information of the female parent fish and the male parent fish; the primer pair is a sequence pair composed of SEQ ID NO. 1 and SEQ ID NO. 2, and a sequence pair composed of SEQ ID NO. 3 and SEQ ID NO. 4. The technical blank of crossbreeding by taking the epinephelus trifoliatus as the parent epinephelus is filled, and the authenticity of the hybridized spots is reliable.

The invention discloses a traditional Chinese medicine composition for repairing and / or activating residual ovarian functions of an aging ovary, and a preparation method and application thereof, and relates to the technical field of biology. The traditional Chinese medicine composition is prepared from the following components: radix astragali, radix angelicae sinensis, radix ophiopogonis, radix polygonati officinalis, semen cuscutae, radix morindae officinalis, fructus rubi, rhizoma dioscoreae and folium mori. The invention further provides application of the traditional Chinese medicine composition in repairing and / or activating the residual ovarian function of the senescent ovary and improving the quality of the senescent oocyte, the traditional Chinese medicine composition can increase the volume and weight of the senescent ovary, increase the number of follicles of all levels in the senescent ovary, reduce apoptosis of granular cells in the ovary and improve the survival rate of the senescent ovary. The abnormal rate of oocytes, oxidative stress of the oocytes and DNA damage are reduced, development and maturation of residual follicles are promoted and maintained, and the traditional Chinese medicine composition has a wide application prospect.

The invention relates to the field of distant hybridization breeding, and discloses an artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus, which comprises the following steps: selecting healthy humpback perches as female parent fishes, and screening healthy epinephelus fuscoguttatus as male parent fishes; carrying out intensive cultivation on the parents by means of nutrient enrichment and temperature regulation and control, and promoting synchronous development and maturation of gonads of the parents; after the parents reach the breeding state, eggs of the female parent fishes and seminal fluid of the male parent fishes are collected respectively, fertilized eggs are obtained through artificial insemination, incubation management such as temperature control and continuous oxygenation is conducted on the fertilized eggs, and finally hybrid grouper fries are obtained. The crossbreeding application of the humpback perch as the female parent and the epinephelus fuscoguttatus is realized, and the problems that in the traditional hybridization process, parent gonad development is asynchronous, and filial generations cannot develop are effectively solved; the bred hybrid provides technical guarantee for standardization and precision promotion of grouper crossbreeding, and creates favorable conditions for breeding new hybrid lines with excellent characters of parents.

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition for relieving pain, promoting blood circulation, diminishing swelling and removing blood stasis, application thereof and a blood stasis dispersing and pain relieving patch. The invention provides a traditional Chinese medicine composition for relieving pain, activating blood, diminishing swelling and removing stasis. The traditional Chinese medicine composition comprises the following components: lycopodium clavatum, radix clematidis, asarum, gentiana macrophylla, radix angelicae, rheum officinale, notopterygium root, roasted herba epimedii, radix achyranthis bidentatae, peach kernel, radix angelicae pubescentis, vinegar-processed rhizoma corydalis, vinegar-processed rhizoma cyperi, moutan bark, prepared kusnezoff monkshood root, prepared monkshood, vinegar-processed frankincense, Sinkiang lithospermum, vinegar-processed myrrh and sanguisorba officinalis. The blood stasis dispersing and pain relieving patch has the advantages of being good in curative effect, quick in effect taking, convenient to use, safe and reliable, can be used for treating traumatic injuries and pain caused by cervical spondylosis, scapulohumeral periarthritis, lumbar disc herniation, lumbar vertebrae hyperosteogeny, spinal stenosis, sciatica, ankylosing spondylitis, arthritis and other diseases, and can achieve the effect of treating both symptoms and root causes.

The invention discloses a dairy cow timed insemination method for controlling ovulation time and improving embryo implantation rate, and relates to the technical field of dairy cow breeding. The problems that follicles on ovaries of postpartum cows are not synchronous in development, ovulation time cannot be accurately regulated and controlled, and embryonic development regulation and control after fertilization are lacked are solved. The method mainly comprises the following steps: three times of gonadotropin releasing hormone injection, two times of cloprostenol sodium injection, gonadotropin releasing hormone injection, luteinizing hormone releasing hormone A3 injection, timed artificial semen deposition, and VADE mixed liquid injection. GnRH is used for actively regulating follicular development, 95% or more of the dairy cows are synchronized after 2-3 rounds of starting, then timing insemination is performed, the conception rate of the postpartum dairy cows can be remarkably increased, compared with the prior art, the pretreatment time is short, the synchronous ovulation rate and conception rate of the dairy cows can be increased, the calving interval is shortened, and the survival rate of the dairy cows is increased. The economic benefit of the pasture is obviously increased, and the breeding management level can be improved on the basis.

The invention discloses an anti-aging longevity and qi-boosting soup composition and its use, comprising the following raw materials or extracts of raw materials in parts by weight: 9-30 parts of Radix Pseudostellariae, 9-30 parts of Poria, 6-20 parts of Angelicae Sinensis, 6-20 parts of stir-fried Semen Ziziphi Spinosae, 6-15 parts of Radix Ophiopogonis, 3-15 parts of roasted Polygala Tenuifolia, 10-60 parts of Longan Meat, 10-30 parts of Radix Rehmanniae, 9-30 parts of Scrophulariae, 3-20 parts of Acorus Gramineus, 9-30 parts of Semen Platycladi, 15-30 parts of roasted Plastron Tortoise, 15-30 parts of calcined Dragon Bone, 6-30 parts of Lotus, and 3-60 parts of Licorice. The composition can inhibit the activity of aging markers β-galactosidase in cells and animals, the expression of P16 or P21, remove senescent cells, improve the antioxidant capacity of cells and animals, reduce the expression level of inflammatory factors in cells and animals, and has a significant anti-aging effect.

The utility model relates to an auxiliary breeding device for livestock breeding veterinarians, and aims to solve the technical problems that the clamping force of a breeding cage is difficult to control, and semen flows back after flowing into a sow body through a vagina, so that the breeding semen is wasted, and the semen cannot enter the sow body conveniently. Comprising a bottom plate and a cage body welded and fixed to the top of the bottom plate, the bottom plate is matched with the cage body to form a breeding cage with an inlet, and a hind limb lifting assembly facing the inlet and used for lifting the hind limbs of livestock is installed between the bottoms of the two sides in the cage body in a centrally hinged mode; according to the livestock breeding cage, the movement of livestock is further limited by fixing and lifting the hind limbs, the livestock can be limited easily, the problem that semen flows back after being injected is solved, and the livestock breeding cage has the advantages that the livestock breeding cage is simple in structure, convenient to use and high in practicability. The phenomenon of waste of the breeding semen is avoided, and the semen can be helped to enter the livestock body through the vagina.

The invention discloses a sheep semen diluent formula and a freezing method, and relates to the technical field of animal breeding, and every 100 mL of diluent is prepared from 0.28 g of functional amino acid, 0.07 g of bioactive peptide, 0.30 g of a buffering agent, 4.12 g of an osmotic pressure regulator and the balance ultrapure water. The preservation effect of the sheep semen is remarkably improved by accurately proportioning the functional amino acid, the bioactive peptide, the buffering agent and the osmotic pressure regulator, particularly, the specific concentration and proportion of the functional amino acid and the bioactive peptide provide necessary nutrition support for the semen, the activity and the survival rate of the semen are also promoted, and the survival rate of the semen is increased. By means of the innovative formula design, the sheep semen can still keep high quality after being diluted, a solid foundation is laid for follow-up cryopreservation, comprehensive protection of the biological activity of the sheep semen is achieved through careful component selection and concentration optimization, and the limitation of traditional diluents in component and effect is broken through.

The invention belongs to the field of fish germplasm improvement and innovation, and discloses an application of river perch sperms in inducing gynogenesis of largemouth bass, which comprises the following steps: obtaining mature largemouth bass ova and river perch sperms through artificial spawning induction; the method comprises the following steps: collecting river perch semen, diluting, oscillating, irradiating and inactivating with an ultraviolet lamp, mixing with mature largemouth bass ova, inseminating, and hatching fertilized ova to obtain gynogenesis largemouth bass. According to the method, the sperms of the river perches are used for stimulating gynogenesis of the micropterus salmoides for the first time, the gynogenesis micropterus salmoides is successfully obtained, and the heterologous sperms in the far geographic space are used as the stimulation source of the artificial gynogenesis ovum for the first time. The method not only provides an innovative germplasm resource basis in the aspect of improving germplasm of the micropterus salmoides, but also has important significance in the research aspects of fish genetic breeding and'micro-hybridization among fish species in far geographic space '.

The present application relates to the technical field of aided conception. Provided is a conception-aiding sheath, comprising a main rubber sheath and a glans portion, the main rubber sheath being cylindrical and having an opening at a lower end surface thereof, a rubber tightening ring being provided on an outer side of the opening, and the glans portion being located at the end of the main rubber sheath away from the opening and connected to the main rubber sheath via a fastening ring; and further comprising a sperm storage portion, which is made of rubber, is harder than the glans portion, and has a containment volume of 2 ml, wherein a sperm gathering ring is provided at a lower end of the sperm storage portion and is fixedly connected to the glans portion, the sperm storage portion is configured to temporarily store sperm, and the sperm gathering ring is configured to prevent sperm from overflowing to the glans portion; and holes are formed in an upper end surface of the sperm storage portion, nine holes are provided, and the holes are configured to constrict the semen flow stream to increase the pressure. The present application has the advantages of increasing the sperm flow velocity, shortening the contact time between sperm and acidic environments, and improving the conception rate.

The invention discloses a rapid sperm screening device and a forming method, the rapid sperm screening device comprises a working shell, a glass slide and at least one layer of screening assembly, and the working shell is provided with a first through groove and a second through groove; the working shell is arranged on the glass slide, so that a sample adding area is formed between the first through groove and the surface of the glass slide, a sample discharging area is formed between the second through groove and the surface of the glass slide, a containing concave position is arranged between the bottom of the working shell and the glass slide, the containing concave position is located between the first through groove and the second through groove, and the screening assembly is arranged in the containing concave position. Compared with the prior art, the sperm screening device has the beneficial effects that the sperm screening device is portable, can be operated in a common clinic or a home environment, completely depends on swimming of active sperms, the screened sperms have high DNA integrity, damage to the DNA integrity of the sperms is small, meanwhile, an integrated screening process is achieved, and the screening efficiency is improved. The seminal fluid is effectively prevented from being polluted in the whole assisted reproduction process.

The invention discloses a composition for treating hyperuricemia nephropathy and application thereof, and belongs to the field of biological medicines.The composition is prepared from, by weight, 28-32 parts of rhizoma smilacis glabrae, 28-32 parts of yam rhizome, 13-17 parts of radix clematidis, 8-12 parts of peach kernels, 8-12 parts of flos carthami, 8-12 parts of herba lycopi, 18-22 parts of rhizoma alismatis, 28-32 parts of raw coix seeds, 28-32 parts of semen plantaginis, 13-17 parts of rhizoma atractylodis and 13-17 parts of pseudobulbus cremastrae seu pleiones. The composition can obviously reduce the uric acid level of a mouse with high uric acid nephropathy and improve kidney injury; meanwhile, intestinal flora of mice with the hyperuricemia nephropathy can be remarkably remodeled, abundance of beneficial bacteria Ackerman's bacteria is up-regulated, abundance of opportunistic pathogenic bacteria such as escherichia coli-shigella, desulfurization vibrio and enterococcus is down-regulated, and important significance is achieved for research and drug development of the hyperuricemia nephropathy.

The invention provides a method for on-site rapid artificial insemination of wild sal-family large yellow croakers, and belongs to the technical field of aquatic animal breeding. According to the method, wild parent fishes are captured in the breeding season of the sal-family pseudosciaena crocea; selecting sexually mature male parent fishes on a capturing site, and collecting seminal fluid for later use; after manual semen collection is finished, female parent fishes with sexual maturity are selected, and fish eggs are collected; performing artificial insemination to obtain high-quality fertilized eggs; the artificial insemination comprises the following steps: adding seminal fluid into the roes, then adding natural seawater, stirring, and finally washing with the natural seawater. According to the method, key links of the whole artificial insemination process are comprehensively regulated and controlled, so that the purposes of obtaining high-quality fertilized eggs and carrying out artificial fry breeding by utilizing the parent fish of the wild sal ammoniac large yellow croaker are achieved.

The invention discloses a method for improving the application efficiency of frozen semen of sheep, and relates to the field of animal reproduction and breeding science, and the method comprises the following steps: collecting semen, freezing half of the semen, then respectively carrying out exosome extraction and identification experiments, then carrying out pandorah sequencing on small non-coding RNA (Ribonucleic Acid), and finally obtaining the application efficiency of the frozen semen of the sheep. The small non-coding RNA in fresh semen and frozen semen is qualitatively and quantitatively analyzed, differential expression microRNAs are screened out, corresponding target genes and signal channels are analyzed, and the small non-coding RNA and the signal channels related to semen cryopreservation are screened out. Finally, the differentially expressed small non-coding RNAs are added into frozen semen in a manner of in-vitro chemical synthesis and the like, or are added in an auxiliary manner during in-vitro artificial insemination, or are delivered through lipidosome, so that the phenotype of an embryo after insemination is influenced, the conception rate of in-vitro artificial insemination is increased, the problem in practical production is favorably solved, and the economic benefit is increased. And economic values are created for enterprises.

According to the Hainan pig semen preservation diluent as well as the preparation method, the application and the detection method thereof disclosed by the invention, galangin and kaempferide are added into a basic diluent to develop a diluent formula capable of efficiently preserving semen at a low temperature (4 DEG C); the galangin and the kaempferol added into the diluent have a good sperm protection effect and a certain antibacterial ability and can replace antibiotics, and bovine serum albumin (BSA) is further combined as a low-temperature protective agent, so that sperm movement parameters are improved, and the low-temperature preservation time of the boar semen is effectively prolonged; the use amount of antibiotics in pig raising production is reduced, the problem of drug resistance of bacteria can be effectively relieved, and the use of the antibiotics and the generation of drug-resistant bacteria are greatly reduced. According to the method, the semen utilization rate of excellent breeding boars is increased, sow health is guaranteed, the conception rate of deep insemination is increased, and efficient and low-cost artificial insemination is achieved.

The invention discloses a breeding method and device for breeding boars, belongs to the technical field of breeding of the boars, and aims to solve the problems that in order to prevent seminal fluid from flowing out after insemination, an external air source is often relied on, so that discomfort of sows is easily caused, and the success rate of fertilization is influenced. The problem that the pregnancy effect is affected due to the fact that a pipeline is left in the body for a long time and discomfort of a pig is easily caused is solved, the gel suppository can be attached to the irregular mucous membrane form of the cervix uteri inner opening after being expanded, leakage caused by reserved gaps is avoided, the expansion degree does not need to be manually controlled, the error-tolerant rate is higher, and the application range is wide. Meanwhile, the plugging mechanism of the gel suppository is made of a natural degradation material, can be naturally degraded and removed or discharged along with uterus peristalsis, has no long-term retention risk, can improve the vagina flushing effect, can simulate the action during mating in the vagina, facilitates fertilization reaction of sows and is more beneficial to successful fertilization.

The utility model discloses an electronic tag for semen cryopreservation recording, which belongs to the technical field of livestock breeding and comprises a power supply module, a main controller, a low-power-consumption power supply control module, a temperature sensor, an RFID (radio frequency identification) module, a memory A, a memory B, a memory C and a memory D, according to the utility model, the technical problem that the electronic tag for semen cryopreservation records realizes low power consumption design and further realizes tamper-proof design through a low-cost logic circuit is solved, temperature data during the preservation period can be accurately measured, and the storage space is expanded; the temperature data generated during the storage period can be reliably stored for a long time, the electric energy of a battery is saved to the maximum extent, the energy consumption is reduced, through the design of a low-cost logic circuit, the safety protection of the stored data is realized, the data tampering difficulty is effectively increased, and the reliability and integrity of the data are ensured.

The present application relates to a method and system for inferring the age of an individual from semen or a semen stain, and a methylation detection kit, which comprises the following steps: firstly, extracting the genomic DNA of the semen or semen stain of the individual; secondly, performing bisulfite treatment on the genomic DNA; thirdly, selecting a plurality of sites in the CpG sites in the genomic DNA and amplifying the selected CpG sites to obtain the methylation rate of the selected CpG sites; and finally, performing regression analysis on the methylation rate of the selected CpG sites and the age of the individual to construct a regression model for inferring the age of an unknown individual from semen or a semen stain. The average absolute error of the present application reaches 1.68 to 4.44 years.

The invention discloses a traditional Chinese medicine composition for treating esophageal cancer. The traditional Chinese medicine composition is prepared from, by weight, 180-210 g of prepared rehmannia roots, 120-150 g of radix clematidis, 100-120 g of rhizoma atractylodis macrocephalae, 120-150 g of fructus lycii, 120-150 g of semen cuscutae, 100-120 g of rhizoma alismatis, 100-120 g of rhizoma dioscoreae, 40-60 g of taxus chinensis, 100-120 g of codonopsis pilosula, 20-40 g of scorpions, 40-60 g of curcuma zedoary, 60-80 g of peach kernels, 120-150 g of salvia chinensis, 120-150 g of rabdosia rubescens, 90-120 g of Chinese dates, 100-120 g of camptotheca acuminate, 30-50 g of ground beetles, 60-80 g of pseudo-ginseng The composition has the effect of treating both symptoms and root causes in the aspect of treating esophageal cancer, can control tumors, improve the living quality of patients and prolong survival, and is small in side effect.

The invention discloses a timed insemination method for improving the pregnancy rate and the lambing rate of sheep, and belongs to the technical field of livestock breeding. In order to solve the problem that the pregnancy rate of existing sheep is unstable during regular insemination, the method comprises the steps that a progesterone sponge suppository is embedded in the vagina of a healthy and reproductive ewe on the 0th day, and meanwhile 25 g of luteinizing hormone is injected in an intramuscular mode to release hormone A3; removing the thrombus on the 12th day, and performing intramuscular injection of 400IU of pregnant mare serum gonadotropin at the same time; and after the suppository is removed, insemination is carried out on the cervix orifice of the ewe with ram semen diluted at the ratio of 1: (5-8) in 52h and 64h respectively. According to the method, ovarian activities of the ewes are regulated and controlled, the estrus synchronism and the ovulation rate are improved, tests show that the pregnancy rate reaches 81.6%, the lambing rate is 1.14, compared with a conventional method, the pregnancy rate and the lambing rate of the sheep are remarkably improved, and development of the sheep industry is promoted.

The application discloses six boar sperm motility miRNA markers, which are ssc-miR-486, ssc-miR-10386, ssc-miR-708-5p, ssc-miR-122-5p, ssc-miR-199a-3p and ssc-miR-31, wherein the ssc-miR-486, ssc-miR-10386, ssc-miR-708-5p, ssc-miR-122-5p and ssc-miR-199a-3p are highly expressed in low-motility semen sperm plasma exosomes and lowly expressed in high-motility semen sperm plasma exosomes, and the ssc-miR-31 is lowly expressed in low-motility semen sperm plasma exosomes and highly expressed in high-motility semen sperm plasma exosomes. The application has good prospects for identifying boar sperm motility, and the disclosed miRNA markers have accuracy and reliability.