233 results about "Semen" patented technology

The present application relates to the field of biotechnology. Provided are a PDRN, and a preparation method therefor and the use thereof. The PDRN has a GC content of 39%-42%. In the PDRN prepared by means of the method recited in the present application, the GC% in the gDNA thereof has a similarity of 98% or more to the GC% of human and is closer to the GC% in a human body than that of a salmon-derived PDRN. The PDRN of the present application has higher safety and efficacy, and compared with the extracting of PDRN from salmon semen in the prior art, the method for preparing the PDRN disclosed in the present application is simpler and more convenient.

The invention discloses an artificial breeding method of epinephelus fuscoguttatus and pink pterus coerulescens hybrid species, which comprises the following steps: screening healthy epinephelus fuscoguttatus as a female parent, screening healthy pink pterus coerulescens as a male parent, and carrying out enhanced breeding on the male parent and the female parent, the gonad maturation of the parent is promoted; the method comprises the following steps: collecting egg liquid of a female parent and semen of a male parent, inseminating to obtain a fertilized egg, incubating the fertilized egg to obtain a hybrid spot, performing authenticity identification on the hybrid spot by using one or more of sequences SEQ ID NO.1-SEQ ID NO.8, and determining that the hybrid spot inherits genetic information of the female parent and the male parent. The technical blank of crossbreeding of the pink pink bass as the parent grouper is filled, so that the hybrid obtained by hybridizing the epinephelus fuscoguttatus and the pink pink bass is reliable in authenticity, and inherits genetic information of male and female parents on the molecular level.

The invention discloses an artificial cultivation method of a hybrid of epinephelus trifoliatus and epinephelus lanceolatus, which comprises the following steps: taking healthy epinephelus trifoliatus as female parent fish, and carrying out cultivation strengthening and artificial ripening to obtain strengthened female parent fish; healthy epinephelus lanceolatus is used as male parent fish, and sexual mature male parent fish is obtained through natural sexual maturity; the ovulation time and the spermiation time are consistent; collecting egg liquid and seminal fluid, inseminating to obtain fertilized eggs, and performing embryo incubation and illumination regulation and control cultivation after opening to obtain hybrid spots; carrying out authenticity identification on the hybrid spots by utilizing a primer pair, and determining that the hybrid spots inherit genetic information of the female parent fish and the male parent fish; the primer pair is a sequence pair composed of SEQ ID NO. 1 and SEQ ID NO. 2, and a sequence pair composed of SEQ ID NO. 3 and SEQ ID NO. 4. The technical blank of crossbreeding by taking the epinephelus trifoliatus as the parent epinephelus is filled, and the authenticity of the hybridized spots is reliable.

The invention relates to the field of distant hybridization breeding, and discloses an artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus, which comprises the following steps: selecting healthy humpback perches as female parent fishes, and screening healthy epinephelus fuscoguttatus as male parent fishes; carrying out intensive cultivation on the parents by means of nutrient enrichment and temperature regulation and control, and promoting synchronous development and maturation of gonads of the parents; after the parents reach the breeding state, eggs of the female parent fishes and seminal fluid of the male parent fishes are collected respectively, fertilized eggs are obtained through artificial insemination, incubation management such as temperature control and continuous oxygenation is conducted on the fertilized eggs, and finally hybrid grouper fries are obtained. The crossbreeding application of the humpback perch as the female parent and the epinephelus fuscoguttatus is realized, and the problems that in the traditional hybridization process, parent gonad development is asynchronous, and filial generations cannot develop are effectively solved; the bred hybrid provides technical guarantee for standardization and precision promotion of grouper crossbreeding, and creates favorable conditions for breeding new hybrid lines with excellent characters of parents.

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition for relieving pain, promoting blood circulation, diminishing swelling and removing blood stasis, application thereof and a blood stasis dispersing and pain relieving patch. The invention provides a traditional Chinese medicine composition for relieving pain, activating blood, diminishing swelling and removing stasis. The traditional Chinese medicine composition comprises the following components: lycopodium clavatum, radix clematidis, asarum, gentiana macrophylla, radix angelicae, rheum officinale, notopterygium root, roasted herba epimedii, radix achyranthis bidentatae, peach kernel, radix angelicae pubescentis, vinegar-processed rhizoma corydalis, vinegar-processed rhizoma cyperi, moutan bark, prepared kusnezoff monkshood root, prepared monkshood, vinegar-processed frankincense, Sinkiang lithospermum, vinegar-processed myrrh and sanguisorba officinalis. The blood stasis dispersing and pain relieving patch has the advantages of being good in curative effect, quick in effect taking, convenient to use, safe and reliable, can be used for treating traumatic injuries and pain caused by cervical spondylosis, scapulohumeral periarthritis, lumbar disc herniation, lumbar vertebrae hyperosteogeny, spinal stenosis, sciatica, ankylosing spondylitis, arthritis and other diseases, and can achieve the effect of treating both symptoms and root causes.

The invention discloses a dairy cow timed insemination method for controlling ovulation time and improving embryo implantation rate, and relates to the technical field of dairy cow breeding. The problems that follicles on ovaries of postpartum cows are not synchronous in development, ovulation time cannot be accurately regulated and controlled, and embryonic development regulation and control after fertilization are lacked are solved. The method mainly comprises the following steps: three times of gonadotropin releasing hormone injection, two times of cloprostenol sodium injection, gonadotropin releasing hormone injection, luteinizing hormone releasing hormone A3 injection, timed artificial semen deposition, and VADE mixed liquid injection. GnRH is used for actively regulating follicular development, 95% or more of the dairy cows are synchronized after 2-3 rounds of starting, then timing insemination is performed, the conception rate of the postpartum dairy cows can be remarkably increased, compared with the prior art, the pretreatment time is short, the synchronous ovulation rate and conception rate of the dairy cows can be increased, the calving interval is shortened, and the survival rate of the dairy cows is increased. The economic benefit of the pasture is obviously increased, and the breeding management level can be improved on the basis.

The invention discloses a composition for treating hyperuricemia nephropathy and application thereof, and belongs to the field of biological medicines.The composition is prepared from, by weight, 28-32 parts of rhizoma smilacis glabrae, 28-32 parts of yam rhizome, 13-17 parts of radix clematidis, 8-12 parts of peach kernels, 8-12 parts of flos carthami, 8-12 parts of herba lycopi, 18-22 parts of rhizoma alismatis, 28-32 parts of raw coix seeds, 28-32 parts of semen plantaginis, 13-17 parts of rhizoma atractylodis and 13-17 parts of pseudobulbus cremastrae seu pleiones. The composition can obviously reduce the uric acid level of a mouse with high uric acid nephropathy and improve kidney injury; meanwhile, intestinal flora of mice with the hyperuricemia nephropathy can be remarkably remodeled, abundance of beneficial bacteria Ackerman's bacteria is up-regulated, abundance of opportunistic pathogenic bacteria such as escherichia coli-shigella, desulfurization vibrio and enterococcus is down-regulated, and important significance is achieved for research and drug development of the hyperuricemia nephropathy.

According to the Hainan pig semen preservation diluent as well as the preparation method, the application and the detection method thereof disclosed by the invention, galangin and kaempferide are added into a basic diluent to develop a diluent formula capable of efficiently preserving semen at a low temperature (4 DEG C); the galangin and the kaempferol added into the diluent have a good sperm protection effect and a certain antibacterial ability and can replace antibiotics, and bovine serum albumin (BSA) is further combined as a low-temperature protective agent, so that sperm movement parameters are improved, and the low-temperature preservation time of the boar semen is effectively prolonged; the use amount of antibiotics in pig raising production is reduced, the problem of drug resistance of bacteria can be effectively relieved, and the use of the antibiotics and the generation of drug-resistant bacteria are greatly reduced. According to the method, the semen utilization rate of excellent breeding boars is increased, sow health is guaranteed, the conception rate of deep insemination is increased, and efficient and low-cost artificial insemination is achieved.

The invention discloses a breeding method and device for breeding boars, belongs to the technical field of breeding of the boars, and aims to solve the problems that in order to prevent seminal fluid from flowing out after insemination, an external air source is often relied on, so that discomfort of sows is easily caused, and the success rate of fertilization is influenced. The problem that the pregnancy effect is affected due to the fact that a pipeline is left in the body for a long time and discomfort of a pig is easily caused is solved, the gel suppository can be attached to the irregular mucous membrane form of the cervix uteri inner opening after being expanded, leakage caused by reserved gaps is avoided, the expansion degree does not need to be manually controlled, the error-tolerant rate is higher, and the application range is wide. Meanwhile, the plugging mechanism of the gel suppository is made of a natural degradation material, can be naturally degraded and removed or discharged along with uterus peristalsis, has no long-term retention risk, can improve the vagina flushing effect, can simulate the action during mating in the vagina, facilitates fertilization reaction of sows and is more beneficial to successful fertilization.

The present application relates to a method and system for inferring the age of an individual from semen or a semen stain, and a methylation detection kit, which comprises the following steps: firstly, extracting the genomic DNA of the semen or semen stain of the individual; secondly, performing bisulfite treatment on the genomic DNA; thirdly, selecting a plurality of sites in the CpG sites in the genomic DNA and amplifying the selected CpG sites to obtain the methylation rate of the selected CpG sites; and finally, performing regression analysis on the methylation rate of the selected CpG sites and the age of the individual to construct a regression model for inferring the age of an unknown individual from semen or a semen stain. The average absolute error of the present application reaches 1.68 to 4.44 years.

The invention discloses a timed insemination method for improving the pregnancy rate and the lambing rate of sheep, and belongs to the technical field of livestock breeding. In order to solve the problem that the pregnancy rate of existing sheep is unstable during regular insemination, the method comprises the steps that a progesterone sponge suppository is embedded in the vagina of a healthy and reproductive ewe on the 0th day, and meanwhile 25 g of luteinizing hormone is injected in an intramuscular mode to release hormone A3; removing the thrombus on the 12th day, and performing intramuscular injection of 400IU of pregnant mare serum gonadotropin at the same time; and after the suppository is removed, insemination is carried out on the cervix orifice of the ewe with ram semen diluted at the ratio of 1: (5-8) in 52h and 64h respectively. According to the method, ovarian activities of the ewes are regulated and controlled, the estrus synchronism and the ovulation rate are improved, tests show that the pregnancy rate reaches 81.6%, the lambing rate is 1.14, compared with a conventional method, the pregnancy rate and the lambing rate of the sheep are remarkably improved, and development of the sheep industry is promoted.

The application discloses six boar sperm motility miRNA markers, which are ssc-miR-486, ssc-miR-10386, ssc-miR-708-5p, ssc-miR-122-5p, ssc-miR-199a-3p and ssc-miR-31, wherein the ssc-miR-486, ssc-miR-10386, ssc-miR-708-5p, ssc-miR-122-5p and ssc-miR-199a-3p are highly expressed in low-motility semen sperm plasma exosomes and lowly expressed in high-motility semen sperm plasma exosomes, and the ssc-miR-31 is lowly expressed in low-motility semen sperm plasma exosomes and highly expressed in high-motility semen sperm plasma exosomes. The application has good prospects for identifying boar sperm motility, and the disclosed miRNA markers have accuracy and reliability.

The utility model discloses an artificial insemination transplantation device, and relates to the technical field of medical instruments. The device structurally comprises a dredging cylinder, a transplanting hose and an injector, the center of the dredging cylinder is provided with a round through hole for the front end of the transplanting hose to pass through; the transplanting hose comprises a circular hose; a semi-spherical end is integrally formed at the front end of the hose; a connecting cap matched with the injector in an inserting manner is integrally formed at the rear end of the hose; the outer wall of the head of the front end of the hose is provided with a circle of evenly-distributed liquid inlets and outlets. Through the design of the dredging cylinder, the transplanting hose can be quickly and accurately inserted into the deep part of the vagina, so that the operation time is shortened, and the complexity is reduced; the injector is isolated from the transplanting hose through the ball, so that seminal fluid is prevented from being in direct contact with the inner wall of the injector, and the integrity and quality of the seminal fluid are guaranteed; the design of the ball enables the position of the ball in the hose to be adjusted according to needs, so that the flowing speed and quantity of seminal fluid can be conveniently controlled, and meanwhile, the ball assists in emptying the seminal fluid in the transplanting hose.

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a traditional Chinese medicine composition for promoting blood circulation to remove meridian obstruction and a preparation method and application thereof. The traditional Chinese medicine composition for promoting blood circulation to remove meridian obstruction is prepared from the following raw materials in parts by weight: 15 to 25 parts of herba taxilli, 18 to 22 parts of semen cuscutae, 8 to 15 parts of radix angelicae sinensis, 3 to 6 parts of radix salviae miltiorrhizae, 18 to 22 parts of radix dipsaci, 8 to 15 parts of herba leonuri, 12 to 18 parts of radix paeoniae alba, 25 to 35 parts of rhizoma dioscoreae, 6 to 12 parts of rhizoma chuanxiong, 3 to 8 parts of radix aucklandiae, 6 to 12 parts of fructus nelumbinis, 10 to 15 parts of radix scutellariae and 12 to 20 parts of roasted rhizoma atractylodis macrocephalae. The traditional Chinese medicine composition obtained through the composition, the dosage and the proportion of the components can remarkably reduce the whole blood viscosity, hematocrit and plasma viscosity of a rat with a blood stasis syndrome, can remarkably reduce the twisting frequency of an experimental dysmenorrhea model rat, has the effects of activating blood, resisting inflammation, dredging collaterals and relieving pain, and has the effect superior to that of each pair of components.

The invention relates to the technical field of traditional Chinese medicine compositions, and particularly discloses a composition for treating lower limb arteriosclerosis obliterans, which is technically characterized by being prepared from ten medicinal materials, namely red ginseng, astragalus membranaceus, cassia twig, morinda officinalis, cistanche deserticola, salvia miltiorrhiza, pseudo-ginseng, radix cyathulae, sargentgloryvine stem and semen brassicae according to a specific weight part ratio. Aiming at the core pathogenesis of yang deficiency and blood stasis of lower limb arteriosclerosis obliterans, the composition disclosed by the invention provides a treatment method of tonifying qi, warming yang, promoting blood circulation to remove blood stasis, removing speckles and dredging vessels, the compatibility of the whole formula is rigorous, and monarch, minister, assistant and guide are clear; moreover, the composition can be prepared into capsules or granules, is used for treating yang deficiency and blood stasis type lower limb arteriosclerosis obliterans, has an exact clinical curative effect, can remarkably improve symptoms of patients and lower limb blood flow, and is good in safety.

The present invention relates to the field of genetic breeding technology, and specifically discloses a SNP molecular marker, primer, and application related to linear motility of sperm after cryopreservation and thawing of chicken semen. The nucleotide sequence of the SNP molecular marker is shown in SEQ ID NO.1, and the 53rd position in the SNP molecular marker is a SNP site, which has a G / A polymorphism. The SNP molecular marker provided by the present invention has a SNP site at position 53, which has a G / A polymorphism. The proportion of linearly motile sperm in semen of individuals with a GA genotype after cryopreservation and thawing of semen is higher than the proportion of linearly motile sperm in semen of individuals with an AA genotype after cryopreservation and thawing of semen.

The invention relates to an animal semen collection system comprising an artificial vagina (2) having an internal space (8) for collecting the semen (5) delimited by a first enlarged end (6) and a second thinned end (7), and a collection tube (3) having an introduction opening (9) and configured to be partially inserted by its opening into the vagina at its second thinned end, the second thinned end having a thinned internal diameter of between about 27 mm and about 28 mm, the collection tube being a standard laboratory tube having a standard external diameter greater than the thinned internal diameter of the artificial vagina at its second thinned end, and the artificial vagina being at least partially elastically deformable at its second thinned end so that the internal space of the artificial vagina opens directly into the introduction opening of the collection tube.Figure for the abbreviation: figure 1.

The sperm optimal tube comprises an upper tube, a lower tube and an inner tube, the upper tube and the inner tube are communicated, the upper tube is detachably connected with the lower tube, when the upper tube is connected with the lower tube, the lower tube is located outside the inner tube, one end of the inner tube is communicated with the upper tube, and the other end is provided with an opening allowing sperm to pass through, and a switch valve is arranged on the opening, the switch valve can be opened under the action of centrifugal force and closed after the centrifugal force stops. The device can effectively separate normal and active sperm, and especially because the switch valve is arranged, the sperm is allowed to flow out under the action of centrifugal force, and the switch valve is closed to seal the inner tube when the centrifugal force is eliminated, so that the abnormal sperm, the inactivated sperm and other cell components in the semen in the inner tube are prevented from entering the lower tube, and the complete separation of sperm with different activities is realized; the design of the inner tube, the upper tube and the lower tube facilitates the disassembly of the outer tube, the semen is more convenient to take out, and the mutual interference is avoided.

The invention relates to the technical field of poultry farming, and discloses a goose massage semen collection device and a using method, the goose massage semen collection device comprises a fixing table, an arc-shaped concave part is arranged on the fixing table, and an arc-shaped outer frame is fixed on the outer side of the fixing table; the shading head and neck fixing pipe is fixed to the fixing table and located on the left side of the arc-shaped concave part, the shading head and neck fixing pipe is used for constraining the head and neck of the male goose so as to reduce the stress response of the male goose, the arch-shaped climbers are located on the fixing table and located on the front side and the rear side of the arc-shaped concave part, and constraining pieces for fixing goose limbs are installed on the arch-shaped climbers; the nervous mood of the male goose is reduced through the shading head and neck fixing pipe, meanwhile, the stimulation process in the natural mating environment is simulated through the back massage mechanism, the stimulation effect of sexual reflex is improved, meanwhile, the designed cloaca bionic mechanism can be accurately in butt joint with a semen discharge opening, and the sexual reflex effect is improved. Semen discharge is further promoted through the alternate inflation and deflation function of the pinching and pressing air bag, and therefore the semen collection quality and quantity are guaranteed.

The invention belongs to the technical field of biology, and particularly discloses a chicken semen cryopreservation solution and a preparation method thereof. The chicken semen cryopreservation liquid comprises a cryoprotection liquid I and a cryoprotection liquid II. The freezing protection liquid I is prepared from the following components: glucose, sodium glutamate, potassium acetate, dipotassium phosphate, magnesium acetate, N, N-bis (2-ethoxyl)-2-aminoethanesulfonic acid and an antibiotic solution. And the freezing protection liquid II comprises dimethylformamide and nano zinc oxide. The invention discloses a chicken semen cryopreservation solution and a preparation method thereof, by applying the chicken semen cryopreservation solution, the fertilization and incubation performance of frozen semen is remarkably improved, centrifugation-free and direct semen deposition of the frozen semen are realized, and physical damage and activity reduction caused by the centrifugation process to sperms are avoided.

The utility model provides an animal sperm injector convenient to use, and relates to the technical field of animal artificial insemination. The animal semen injection device convenient to use comprises a semen injection supporting rod body, a semen injection elastic outer sleeve, an easily-folded leakage-proof piece and a blocking piece. The sperm injection elastic outer sleeve is arranged on the sperm injection supporting rod body in a sleeving mode, the two ends of the sperm injection elastic outer sleeve are connected with the sperm injection supporting rod body in a sealed mode, and a containing cavity used for containing sperm is formed between the sperm injection supporting rod body and the sperm injection elastic outer sleeve; the front end of the sperm injection supporting rod body is provided with a first mounting hole for mounting the easy-to-break leakage-proof piece, and the first mounting hole is communicated with the accommodating cavity; a second mounting hole for mounting the blocking piece is formed in the tail end of the semen injection supporting rod body, and the second mounting hole is communicated with the containing cavity; and temperature control wax is arranged in the easy-to-fold leakage-proof piece. The technical effect that the fertilization device is convenient to use is achieved.

The invention discloses a medicine for treating the heart of a human body, and particularly relates to the field of medicines, the medicine is prepared from the following components in percentage by mass: 13.8% of radix salviae miltiorrhizae, 4.1% of allium macrostemon, 8.3% of semen trichosanthis, 2% of polygala tenuifolia, 8.3% of radix ophiopogonis, 2% of sandalwood, 6.9% of pericarpium citri reticulatae viride, 13.8% of lily, 8.3% of semen boitae, 10.4% of poria cocos, 8.3% of oriental cherry powder and 13.8% of earthworm, and the sum of the mass percentages of the components is 100%. According to the medicine for treating the heart of the human body, traditional Chinese medicine decoction pieces such as salvia miltiorrhiza, allium macrostemon, snakegourd seeds, polygala tenuifolia, radix ophiopogonis, sandalwood, pericarpium citri reticulatae viride, lily, platycladi seeds, poria cocos, oriental cherry powder and lumbricus are crushed and proportioned according to corresponding formula proportions and processes, so that the medicine plays the maximum effects of all the decoction pieces, is easier to take and absorb, and can be taken for a long time after being taken. The traditional Chinese medicine composition has obvious treatment effects on cardiac insufficiency, dyspnea, weakness, fatigue, angina pectoris, palpitation, palpitation and other problems caused by heart problems of heart disease patients, and can effectively treat coronary heart disease, myocardial ischemia, arrhythmia and other heart diseases.

The present application belongs to the technical field of dermatosis treatment products, and particularly relates to a soft pelleted medicine and a preparation method thereof. The soft pelleted medicine is prepared from the following raw materials in parts by weight: 20-40 parts of Ramulus cinnamomi, 25-35 parts of Radix astragali, 5-15 parts of Semen persicae, 5-15 parts of Ophiopogon, 5-15 parts of Carthamus tinctorius, 15-30 parts of calcined Radix paeoniae alba, 10-20 parts of Radix angelicae sinensis, 15-30 parts of Radix rehmanniae preparata, 20-40 parts of Radix codonopsis, 5-15 parts of Radix paeoniae alba, 5-10 parts of Ephedra, 5-10 parts of Radix rubiae, 5-15 parts of Centella asiatica, 2-5 parts of Radix et Rhizoma aconiti, 5-10 parts of Salvinia natans, and 5-10 parts of Rhizoma zingiberis. The soft pelleted medicine provided by the present application has the main effects of warming yang and benefiting qi, promoting blood circulation to remove meridian obstruction, dispelling cold and dampness, removing blood stasis and promoting tissue regeneration, and is used for treating dermatosis such as scleroderma, frostbite, Raynaud's disease, skin atrophy, and the like, and particularly has remarkable effects on scleroderma, frostbite, Raynaud's disease, and skin atrophy.

The invention relates to the technical field of breeding regulation and control of black goats, in particular to a hormone treatment method for inducing estrus synchronization of black goats, which comprises the following steps: placing a CIDR vaginal suppository containing 1.38-1.56 g of progesterone into the vagina of an ewe, and keeping for 12-14 days; 48 hours before the thrombus is removed, intramuscularly injecting a sustained release preparation of 330 IU follicle-stimulating hormone and 30% polyvinylpyrrolidone; when the thrombus is removed, intramuscularly injecting a mixed solution containing 330IU pregnant mare serum gonadotropin and 0.1 mg of cloprostenol; 24 hours after the thrombus is removed, 25 micrograms of luteinizing hormone is intravenously injected to release hormone A3; single-time artificial insemination is performed within 48-56 hours after injection, 0.5 ml of semen is left for each injection, and the number of effective sperms is not less than 1.5 * 10 < 8 >. By precisely regulating and controlling CIDR progesterone and multi-hormone stepped injection, the estrus synchronization rate of a group is larger than or equal to 95%, and the traditional synchronization bottleneck is broken through; insemination parameters are controlled and optimized management is carried out, each fetal live lamb is 2.5-3.0, and the breeding efficiency is improved; treatment is started 45-50 days after delivery, the lambing interval is 210 + / -10 days, and large-scale efficient breeding is assisted.

The invention relates to the technical field of poultry breed conservation biology, and discloses a cryopreservation kit for chicken semen and a use method of the cryopreservation kit. The invention relates to a cryopreservation kit for chicken semen. The cryopreservation kit comprises a cryopreservation diluent, a cryoprotectant, an insemination diluent, a DMEM (dulbecco's modified eagle medium) and a frozen semen tube. The cryopreservation diluent in the kit provided by the invention can provide a better living environment for chicken sperms, and the cryoprotectant in the kit can successfully perform cryopreservation on the chicken sperms; when the kit provided by the invention is used for freezing and storing chicken semen, the unfrozen chicken semen is used for artificial insemination, the fertilization rate can reach 70% or above, and the requirement for breed conservation of chicken genetic resources is met.

The invention discloses a stagnation-removing composition as well as a preparation method and application thereof, on the basis of four-adverse-qi powder consisting of radix bupleuri, immature bitter orange, red peony root and honey-fried licorice root, liver and spleen qi is regulated and smoothened, and the ascending and descending functions of intestinal tracts are recovered; the fructus kochiae, the fructus cnidii, the fried peach kernels, the fried semen armeniacae amarae, the Chinese waxgourd seeds fried with bran, the ligusticum wallichii, the lumbricus and the schizonepeta spike are creatively combined, so that the effects of promoting blood circulation to remove blood stasis, promoting diuresis to dispel wind and relaxing bowel are synergistically achieved. According to the traditional Chinese medicine composition, the difficulty in defecation can be effectively improved, the pathological links of qi stagnation, damp obstruction and blood stasis can be intervened from the pathogenesis, various uncomfortable symptoms related to abdominal fullness, anus falling expansion, vector qi frequency and the like can be remarkably relieved, the conversion from treatment according to noses to treatment for the root is realized, and a solution with more comprehensive effects and more lasting curative effects is provided.

The present application belongs to the technical field of fish breeding, and discloses a method for efficiently inducing and rapidly identifying homologous tetraploid crucian male-nuclear development offspring, comprising the following steps: inducing parturition of female hybrid crucian and male homologous tetraploid crucian, obtaining hybrid crucian ovum, placing the ovum in ovary liquid, inactivating the ovum through ultraviolet lamp irradiation, stirring and uniformly mixing the ovum with sperm of the homologous tetraploid crucian to make the ovum "fertilized", without chromosome doubling treatment, and obtaining survival offspring after hatching and feeding, wherein a pair of oral papillae in the survival offspring is the male-nuclear development offspring. The hybrid crucian ovum selected by the present application contains a heterologous genome itself, has better compatibility, is beneficial to improving the hatching rate, can efficiently induce homologous tetraploid crucian male-nuclear development, and simultaneously utilizes the appearance characteristic of the male-nuclear development individual inheriting a pair of oral papillae from the father to rapidly identify the male-nuclear development offspring, so that the goal of rapidly identifying homologous tetraploid crucian male-nuclear development offspring is achieved, and the present application has important significance in fish genetic breeding.

According to the thin tube processing mechanism and the animal frozen semen unfreezing device, a first transmission mechanism is connected through a fixing plate, a cutting module conducts cutting in a mutual attaching mode, a thin tube is cut in a clamping mode, and the problem that the thin tube shakes in the cutting process is avoided; after the number of frozen fine tubes needed by each first tank body is determined, a clamping device firstly clamps one fine tube, water on the surface of the fine tube is wiped off after water bath unfreezing operation is conducted on the fine tube, then the fine tube is cut off, the fine tube is clamped through a clamping assembly, and the fine tube is moved to the position between a movable plate and a bearing plate after being vertically moved. When animal frozen semen in the thin tube needs to be transferred, the whole thin tube processing mechanism is moved to the position above the receiving mechanism to conduct cutting operation, the arrangement mode needs a small space, meanwhile, unfreezing time can be controlled, the thin tube processing mechanism is vertically moved into the cutting device in the vertical direction to be cut after unfreezing, and after the thin tube is cut, the thin tube processing mechanism is moved to the position above the receiving mechanism to conduct cutting operation. Blowing the unfrozen animal frozen semen in the slim tube into the first tank body filled with the diluent, and circulating until the slim tube required by each bottle of the first tank body is reached; and then the operation of the next bottle is carried out.