An anti-aging longevity-invigorating qi-replenishing soup composition and its use

Abstract

The invention discloses an anti-aging longevity and qi-boosting soup composition and its use, comprising the following raw materials or extracts of raw materials in parts by weight: 9-30 parts of Radix Pseudostellariae, 9-30 parts of Poria, 6-20 parts of Angelicae Sinensis, 6-20 parts of stir-fried Semen Ziziphi Spinosae, 6-15 parts of Radix Ophiopogonis, 3-15 parts of roasted Polygala Tenuifolia, 10-60 parts of Longan Meat, 10-30 parts of Radix Rehmanniae, 9-30 parts of Scrophulariae, 3-20 parts of Acorus Gramineus, 9-30 parts of Semen Platycladi, 15-30 parts of roasted Plastron Tortoise, 15-30 parts of calcined Dragon Bone, 6-30 parts of Lotus, and 3-60 parts of Licorice. The composition can inhibit the activity of aging markers β-galactosidase in cells and animals, the expression of P16 or P21, remove senescent cells, improve the antioxidant capacity of cells and animals, reduce the expression level of inflammatory factors in cells and animals, and has a significant anti-aging effect.

Description

Technical Field

[0001] The invention relates to the fields of medicine and cosmetics, and in particular to an anti-aging longevity-invigorating qi-replenishing soup composition and application thereof. Background Art

[0002] The statements in this section merely provide background information related to the present disclosure and may not constitute prior art.

[0003] Aging is the degenerative change of the overall function of the body with age. Aging is an extremely complex biological process, and its mechanism involves theories such as gene mutation, telomere loss, somatic mutation, free radical damage, immune dysfunction, mitochondrial dysfunction and autophagy dysfunction. Cardiovascular disease, cancer, cataracts, osteoporosis, hypertension, and neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease are all related to aging. Against the background of an aging population, many countries have entered an aging society, which poses a serious threat to economic development and human life and health. Therefore, the research on anti-aging drugs is imminent.

[0004] Studies have shown that drug treatment can effectively delay aging and have a positive effect on aging-related diseases. Aspirin and metformin are commonly used synthetic anti-aging drugs, but these drugs also have obvious side effects. Aspirin has an anti-platelet aggregation effect, and long-term use can easily cause bleeding. Patients taking metformin will experience adverse reactions such as diarrhea, nausea, and abdominal discomfort. The discovery and development of anti-aging drugs is difficult and progress is slow. It is challenging to find a safe and effective anti-aging drug. In recent years, Chinese herbal medicine has been considered a safe and effective anti-aging drug with great development potential. Studies have found that a variety of Chinese medicines and their active ingredients can delay aging and prevent age-related diseases. The main mechanism of action of Chinese medicine is antioxidant and anti-inflammatory, and different mechanisms of action are determined according to different organs and functions. A variety of single and compound Chinese medicines show beneficial anti-aging effects in various parts and functions of the body. In order to realize the industrialization of Chinese medicine, give full play to the advantages of Chinese medicine, and develop safe, effective, and innovative anti-aging, longevity, and qi-boosting soup preparations with independent intellectual property rights, it is of great significance for my country to promote the internationalization of the health industry and improve the quality of life of Chinese residents.

[0005] Therefore, an anti-aging longevity-invigorating qi-replenishing soup composition and its use are invented. Summary of the invention

[0006] The purpose of the present invention is to provide an anti-aging Yishou Buqi Decoction composition and its use, which can significantly inhibit the aging of cell models and animal models, in response to the problem that the current anti-aging Yishou Buqi Decoction composition has few formulas.

[0007] The technical solution of the present invention is as follows:

[0008] An anti-aging longevity and qi-boosting soup composition, characterized in that it comprises the following raw materials or extracts of raw materials in parts by weight: 9-30 parts of Pseudostellariae Radix, 9-30 parts of Poria, 6-20 parts of Angelicae Sinensis, 6-20 parts of stir-fried Ziziphus jujuba seeds, 6-15 parts of Ophiopogon japonicus, 3-15 parts of roasted Polygala tenuifolia, 10-60 parts of longan pulp, 10-30 parts of Rehmannia root, 9-30 parts of Scrophulariae ningpoensis, 3-20 parts of Acorus calamus, 9-30 parts of Platycladus chinensis seeds, 15-30 parts of roasted Tortoise shell, 15-30 parts of calcined Dragon bone, 6-30 parts of Lotus, and 3-60 parts of Licorice.

[0009] Preferably, the following raw materials or extracts of raw materials are included in weight parts: 15-25 parts of Pseudostellariae Radix, 15-25 parts of Poria, 15-20 parts of Angelica sinensis, 15-20 parts of stir-fried Ziziphus jujuba seeds, 10-15 parts of Ophiopogon japonicus, 7-15 parts of roasted Polygala tenuifolia, 5-15 parts of longan pulp, 15-25 parts of Rehmannia root, 15-25 parts of Scrophularia ningpoensis, 10-20 parts of Acorus calamus, 15-25 parts of Platycladus orientalis seeds, 15-25 parts of roasted Tortoise shell, 15-25 parts of calcined dragon bone, 6-11 parts of Lotus, and 3-11 parts of Licorice.

[0010] Preferably, the following raw materials or extracts of raw materials are included in weight parts: 17-23 parts of Pseudostellariae Radix, 17-23 parts of Poria, 17-20 parts of Angelica sinensis, 17-20 parts of stir-fried Ziziphus jujuba seeds, 12-15 parts of Ophiopogon japonicus, 9-15 parts of roasted Polygala tenuifolia, 7-13 parts of longan pulp, 17-23 parts of Rehmannia root, 17-23 parts of Scrophularia ningpoensis, 12-18 parts of Acorus calamus, 17-23 parts of Platycladus orientalis seeds, 17-23 parts of roasted Tortoise shell, 17-23 parts of calcined dragon bone, 6-9 parts of Lotus, and 3-9 parts of Licorice.

[0011] According to a preferred embodiment, the lotus is fresh lotus petals that have been freeze-dried for 3-5 days or shade-dried for 2-4 weeks.

[0012] According to a preferred embodiment, the aging is the increase of the activity of β-galactosidase, a marker of aging in vivo, and the high expression of P16 or P21.

[0013] The present application also provides an anti-aging pharmaceutical preparation, including the anti-aging Yishou Buqi Decoction composition as described above.

[0014] According to a preferred embodiment, the aging is the increase of the activity of β-galactosidase, a marker of aging in vivo, and the high expression of P16 or P21.

[0015] According to a preferred embodiment, it further comprises one or more pharmaceutically acceptable excipients.

[0016] According to a preferred embodiment, the composition can be prepared as an external preparation or an internal preparation.

[0017] On the other hand, the present application also provides use of the anti-aging Yishou Buqi Decoction composition as described above in the preparation of anti-aging traditional Chinese medicine or cosmetics.

[0018] The anti-aging Yishou Buqi Decoction composition or the pharmaceutical preparation and cosmetics prepared from the Yishou Buqi Decoction composition provided by the present invention can be applied to mammals, including but not limited to humans, mice, horses, pigs, dogs, cattle, sheep, rabbits, etc.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. An anti-aging Yishou Buqi Decoction composition, which is non-toxic to HaCat cells, has the effects of promoting the proliferation of HaCat cells to enhance their cell activity, significantly reducing the expression of HaCat cell aging markers P16 and P21 genes, inducing apoptosis of HaCat senescent cells and then removing senescent cells, improving the antioxidant capacity of HaCat cells, reducing the expression level of HaCat cell inflammatory factors, and significantly inhibiting cell aging;

[0021] 2. An anti-aging Yishou Buqi Decoction composition. Animal behavioral experiments have shown that the Yishou Buqi Decoction composition of the present invention can improve the spatial learning and memory abilities of aging mice, delay the aging of the mouse kidneys, significantly reduce the expression of the aging markers P16 and P21 genes in mice, improve the antioxidant capacity of mice, and reduce the expression level of inflammatory factors in mice, thus having an anti-aging effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 Effect of Yishou Buqi Decoction on HaCat senescent cells: compared with the model group, ##P<0.01; compared with the 500μg / mL administration group, **P<0.01;

[0023] Figure 2 This is the effect of the Yishou Buqi Decoction of this application on the activity of normal HaCat cells. Note: Compared with the drug-added group, **P<0.01;

[0024] Figure 3 Flow cytometry scatter plots of HaCat cells in different groups of this application;

[0025] Figure 4 Statistical analysis of apoptotic cells in different groups of HaCat cells after 24 hours. Compared with the model group, the blank group was ##P<0.01; compared with the 500μg / mL administration group, the model group was **P<0.01.

[0026] Figure 5The effect of Yishou Buqi Decoction on the expression of aging markers P16 and P21. Note: (A) P16 expression of HaCat cells in different groups; (B) P21 expression of HaCat cells in different groups. Compared with the model group, ## P<0.01; compared with 125μg / mL and 500μg / mL, **P<0.01 in the model group;

[0027] Figure 6 Effect of Yishoubuqi decoction on the antioxidant capacity of HaCaT cells, compared with the model group, ## P<0.01; compared with 62.5μg / mL, 125μg / mL, 250μg / mL, 500μg / mL and 1000μg / mL, **P<0.01, *P<0.05 in the model group;

[0028] Figure 7 Effects of Yishou Buqi Decoction on inflammatory factors in HaCaT cells: compared with the model group, ## P<0.01, ### P<0.001; compared with 500μg / mL, **P<0.01, ***P<0.001 in the model group;

[0029] Figure 8 The β-galactosidase staining sections of the kidneys of mice in different groups, Note: (A) blank group; (B) model group; (C) low-dose group; (D) high-dose group;

[0030] Fig. 9 Effects of Yishou Buqi Decoction on the expression of P16 and P21 in vivo. Note: (A) P16 expression in mice in different groups; (B) P21 expression in mice in different groups. Compared with the model group, ## P<0.01; **P<0.01 compared with the low-dose group and high-dose group in the model group;

[0031] Fig.10 Effect of Yishou Buqi Decoction on the antioxidant capacity of D-galactose-induced aging mice. # P<0.05, ## P<0.01; compared with the high-dose group, *P<0.05, **P<0.01;

[0032] Fig.11 Effect of Yishou Buqi Decoction on inflammatory factors in D-galactose-induced aging mice. Compared with the model group, ## P<0.01, ### P<0.001; compared with the low-dose group, high-dose group and positive control group (VE), the model group had *P<0.05, **P<0.01, ****P<0.0001;

[0033] Fig.12 Comparative Example 1 and Example 1 DPPH, ABTS + Comparison of free radical scavenging ability with FRAP;

[0034] Fig.13 Effects of Comparative Example 1 and Example 1 on the survival rate of HaCaT cells, compared with the drug-added group, *P<0.05, **P<0.01;

[0035] Fig.14 Comparative Example 2 and Example 1 DPPH, ABTS + Comparison of free radical scavenging ability with FRAP;

[0036] Fig.15 Effects of Comparative Example 2 and Example 1 on the survival rate of HaCaT cells, compared between the blank group and the drug-added group, **P<0.01. DETAILED DESCRIPTION

[0037] The features and performance of the present invention are further described in detail below in conjunction with the examples. Unless otherwise specified, the methods mentioned in this application are commonly used methods in the art; unless otherwise specified, the experimental materials used in this application are all commercially available.

[0038] Example 1: Water extract sample preparation

[0039] Weigh the following Chinese medicine pieces according to the following dosages: Radix Pseudostellariae 20g, Radix Poriae 20g, Radix Angelicae Sinensis 20g, stir-fried Semen Ziziphi Spinosae 20g, Radix Ophiopogonis 15g, roasted Radix Polygalae 12g, longan pulp 10g, Radix Rehmanniae 20g, Radix Scrophulariae 20g, Rhizoma Acorus Tatarinowii 15g, Semen Platycladi 20g, roasted Plastron 20g, calcined Bone of Dragon Bone 20g, Flos Nelumboscis 6g, Radix Glycyrrhizae 6g;

[0040] The above Chinese medicines were mixed in proportion, and distilled water 10 times the total weight of the Chinese medicines was added. After soaking for 30 minutes, the mixture was decocted three times, and distilled water 10 times the total weight of the Chinese medicines was added each time. The decoctions from the three decoctions were combined and the residues were filtered. Finally, the filtered decoction was concentrated by rotary evaporator and freeze-dried. 50 mg of the sample was accurately weighed, dissolved in DMEM medium to 50 mg / mL, vortexed and mixed, and diluted with medium to the required concentration, and filtered with a 0.22 μm microporous filter membrane before cell experiments.

[0041] Example 2: In vitro evaluation of the anti-aging activity of the Yishou Buqi Decoction composition

[0042] Test cells: HaCat cells (human immortalized keratinocytes), divided into blank group, model group, and drug-added group. The blank group was HaCat cells plus culture medium, the model group was HaCat cells irradiated with UV light for 30 seconds, and the drug-added group was cells irradiated with UV light for 30 seconds and then added with 7.8125, 15.625, 31.25, 62.5, 125, 250, 500, 1000 μg / mL of Chinese herbal extract.

[0043] 1 In vitro experiments

[0044] 1.1 Validation of the cell senescence model

[0045] First, place a UV lamp at a distance of 15 cm from the cells for 30 seconds, then replace with fresh DMEM medium and continue culturing. After 24 hours of culture, the cells were exposed to Xite TM β-D-galactopyranoside staining was used to detect senescent cells. Figure 1 It can be seen that when Yishou Buqi Decoction was given to cells, the FITC fluorescence intensity decreased significantly, indicating that the number of senescent cells in the cells decreased. At the same time, there was a significant difference between the model group and the blank group, and the aging model was successfully constructed. This shows that Yishou Buqi Decoction reduced the number of senescent cells and had the effect of clearing senescent cells.

[0046] 1.2 Effects of Yishou Buqi Decoction on Normal Cells

[0047] After using Yishou Buqi Decoction, the CCK8 assay results showed that the cell activity did not decrease compared with the blank group. Moreover, under the action of high concentrations of drugs, the cell activity was enhanced ( Figure 2 ). This shows that the drug is not toxic to cells, and high concentrations of the drug promote the proliferation of HaCat normal cells and enhance their cell activity.

[0048] 1.3 Yishou Buqi Decoction induces apoptosis of HaCat senescent cells

[0049] The results of flow cytometry detection of Yishou Buqi Decoction on apoptosis of HaCat senescent cells are as follows Figure 3 and Figure 4 As shown in Figure 2, the cells were divided into three groups: control group, model group, and drug-added group (500 μg / mL). After the drug acted on the senescent cells for 24 hours, Figure 3 It can be clearly seen that the number of cells in the Q2 and Q3 regions of the drug-added group increased significantly. Figure 4 It can be seen that compared with the blank group and the model group, the cell apoptosis rate in the drug-added group was significantly increased, with significant differences. Therefore, Yishou Buqi Decoction can induce apoptosis of HaCat senescent cells and then eliminate senescent cells.

[0050] 1.4 Yishou Buqi Decoction inhibits the expression of P16 and P21 in vitro

[0051] P16 and P21 are typical biomarkers of aging, and both are highly expressed in aging cells. Real-time PCR was used to detect the expression of P16 and P21 in cells treated with Yishou Buqi Decoction. Figure 5 A shows that P16 in the model group showed a high expression state, and the ability to inhibit P16 expression in the drug-added group increased with the increase of drug concentration. Figure 5 B shows that P21 in the model group showed a high expression state, and the ability to inhibit P21 expression in the drug-added group increased with the increase of drug concentration. Therefore, it can be seen that Yishou Buqi Decoction inhibited the expression of aging genes P16 and P21.

[0052] 1.5 Effect of Yishou Buqi Decoction on the Antioxidant Capacity of HaCaT Cells

[0053] Continuous contact between the human body and the outside world will produce too many free radicals, thus destroying the balance between the body's own antioxidants and free radicals, accelerating the aging of the body. Figure 6 It can be seen that with the increase of drug concentration, the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in cells increased significantly, and the content of malondialdehyde (MDA) decreased significantly, indicating that Yishou Buqi Decoction improved the antioxidant capacity of cells.

[0054] 1.6 Effect of Yishou Buqi Decoction on Inflammatory Factors in HaCaT Cells

[0055] Depend on Figure 7 It can be seen that when Yishou Buqi Decoction was given to the cells, the intracellular IL-6 (interleukin 6), TNF-α (tumor necrosis factor) and IL-1β (interleukin 1β) levels decreased significantly, indicating that Yishou Buqi Decoction reduced the expression of intracellular inflammatory factors.

[0056] Example 3: Evaluation of the anti-aging activity of traditional Chinese medicine in mice.

[0057] 72 healthy C57BL / 6 mice with a body weight of 18-20 g, half male and half female, were fed normal food and the experiment was conducted after 1 week of adaptive feeding.

[0058] C57BL / 6 mice were randomly divided into 6 groups: blank group, model group, vitamin E (VE) group, low-dose, medium-dose and high-dose drug groups. Each mouse in the blank group was intraperitoneally injected with 0.3 mL of normal saline, and the other groups were intraperitoneally injected with an equal volume of D-galactose (0.75 g of D-galactose was accurately weighed and dissolved in 25 mL of 0.9% normal saline) once a day for 6 consecutive weeks to establish the model.

[0059] After successful modeling, mice in the low, medium, and high dose groups were gavaged with 0.3 mL of 200, 400, and 800 mg / kg of the drug, respectively; the VE group was gavaged with an equal volume of 100 mg / kg vitamin E; the blank group and the model group were gavaged with an equal volume of saline, once a day for 5 consecutive weeks. The mice were weighed and recorded weekly, and their fur color, mobility, mental state, etc. were observed.

[0060] 1.1Y-maze analysis

[0061] The mice were placed in a Y-maze, and the proportion of the time they spent in the novel arm to the total time was calculated. The results showed that the time that the mice in the successful modeling entered the novel arm was shorter than that of the mice in the blank group, while the time that the mice in the drug-treated group entered the novel arm was significantly longer than that of the model group, indicating that Yishou Buqi Decoction improved the spatial learning and memory ability of aging mice (as shown in Table 1).

[0062] Table 1 The proportion of time that mice in different groups entered the novel arm (%) (x±s, n=7)

[0063]

[0064] Note: Different lowercase letters in the same column indicate significant differences, and the P value between two letters is <0.05.

[0065] 1.2 β-galactosidase staining sections

[0066] Since the kidney is one of the important organs in aging, the β-galactosidase-stained sections of the kidneys of mice in different groups were analyzed. Figure 8 (A) is the blank group; (B) is the model group; (C) is the low-dose group; (D) is the high-dose group. Figure 8 It can be seen that the positive staining in the model group (B) is significantly increased compared with the blank group (A), and the number of positive staining decreases significantly with the increase of the dosage, indicating that Yishou Buqi Decoction can effectively delay the aging of the kidneys.

[0067] 1.3 Yishou Buqi Decoction inhibits the expression of P16 and P21 in vivo

[0068] Kidneys are an important organ in aging. The kidneys of mice in different groups were ground to extract RNA, and then RT-PCR experiments were performed. Fig. 9 A shows that P16 is highly expressed in the model group, and the ability to inhibit P16 expression increases with the increase in drug dosage. Fig. 9 B shows that P21 in the model group is highly expressed, and the ability to inhibit P21 expression in the drug-treated group increases with the increase in drug dose. It can be seen that Yishou Buqi Decoction also inhibits the expression of aging genes P16 and P21 in vivo.

[0069] 1.4 Effect of Yishou Buqi Decoction on Antioxidant Capacity of D-galactose-induced Aging Mice

[0070] Depend on Fig.10 It can be seen that high-dose Yishou Buqi Decoction significantly increased the superoxide dismutase (SOD) activity and catalase (CAT) content in the kidneys of mice, and significantly decreased the malondialdehyde (MDA) content. There was a significant difference between the model group and the blank group, indicating that Yishou Buqi Decoction improved the antioxidant capacity of aging mice.

[0071] 1.5 Effect of Yishou Buqi Decoction on Inflammatory Factors in D-galactose-induced Aging Mice

[0072] Depend on Fig.11 It can be seen that when mice were gavaged with different doses of Yishou Buqi Decoction, it was found that the levels of IL-6 (interleukin 6), TNF-α (tumor necrosis factor) and IL-1β (interleukin 1β) in the mice decreased significantly with the dosage, and there were significant differences, indicating that Yishou Buqi Decoction reduced the expression of inflammatory factors in mice.

[0073] Comparative Example 1

[0074] Prepare "a Chinese medicine composition for nourishing the mind and calming the heart", composed of the following substances: fried jujube kernel 25.5g, raw dragon bone 26.4g, Schisandra chinensis 17g, Polygala tenuifolia 16.5g, red ginseng 8g, Poria 16.5g, raw Rehmannia root 12.5g, Ophiopogon japonicus 11g, lily 25.2g, Angelica sinensis 6.5g, longan pulp 5.8g, white peony root 12.3g, roasted tortoise shell 20g, Albizzia julibrissin bark 9g, Polygonum multiflorum 26.7g, magnetite 7.5g and amber 1.2g.

[0075] The same preparation method as in Example 1 was adopted, the above medicine was soaked in water for half an hour, decocted three times, the three decoctions were concentrated by a rotary evaporator, and finally the concentrated liquid was freeze-dried.

[0076] 1.1 Antioxidant activity

[0077] Depend on Fig.12 It can be seen that when the drug concentration reaches 1000 μg / mL, the DPPH free radical scavenging rate of Comparative Example 1 reaches 80%, but the DPPH free radical scavenging rate of Example 1 reaches 96%. Compared with Comparative Example 1, the DPPH free radical scavenging rate of Example 1 is stronger.

[0078] When the drug concentration reached 1000 μg / mL, the ABTS of Example 1 + The free radical scavenging rate reaches 96%. Fig.12 It can be seen that the ABTS at various concentrations in Example 1 + The free radical scavenging rates were higher than those in Comparative Example 1.

[0079] Depend on Fig.12 It can be seen that the FRAP free radical scavenging rates at all concentrations of Example 1 are higher than those of Comparative Example 1. In summary, the total antioxidant capacity of Example 1 is significantly stronger than that of Comparative Example 1.

[0080] 1.2 Cytotoxicity

[0081] Depend on Fig.13 It can be seen that comparative example 1 is toxic to cells at a concentration of 500 μg / mL and above, and the cell survival rate is reduced by 25% at a concentration of 1000 μg / mL. Example 1 is not toxic to cells, and at the same time, a concentration of 500 μg / mL and above has a proliferative effect on cells, increasing the cell survival rate by 7%.

[0082] In summary, the compound of the present application increases the antioxidant activity with fewer types of compound drugs and does not produce cytotoxicity. The overall antioxidant effect is better than that of Comparative Example 1.

[0083] Comparative Example 2

[0084] Prepare a "Chinese medicine composition for skin anti-aging", consisting of the following substances: 10g of saffron extract, 40g of ginseng extract, 35g of lotus extract, 30g of knotweed extract, 25g of chuanxiong extract, and 25g of angelica extract. Use the same preparation method as in Example 1, soak the above drugs in water for half an hour, decoct three times, concentrate the three decoctions with a rotary evaporator, and finally freeze-dry the concentrated liquid.

[0085] 1.1 Antioxidant activity

[0086] like Fig.14 It can be seen that when the drug concentration reaches 1000 μg / mL, the DPPH free radical scavenging rate of Comparative Example 2 reaches 90%, but the DPPH free radical scavenging rate of Example 1 reaches 96%. It can be seen that compared with Comparative Example 2, Example 1 has a stronger DPPH free radical scavenging rate.

[0087] like Fig.14 It can be seen that when the drug concentration reaches 1000 μg / mL, the ABTS + The free radical scavenging rate reached 96%, and as the concentration increased, the ability to scavenging free radicals continued to increase. + The free radical scavenging rate showed a slow weakening trend after reaching 250 μg / mL.

[0088] Depend on Fig.14 It can be seen that the iron ion reducing ability of Example 1 at each concentration is higher than that of Comparative Example 2.

[0089] 1.2 Cytotoxicity

[0090] The cytotoxicity of Comparative Example 2 was further verified. Fig.15 It can be seen that comparative example 2 is toxic to cells at a concentration of 15.625 μg / mL and above, and the cell survival rate is reduced by 31% at a concentration of 1000 μg / mL. Example 1 is not toxic to cells, and at the same time, a concentration of 500 μg / mL and above has a proliferation-promoting effect on cells, increasing the cell survival rate by 7%.

[0091] In summary, the compound of the present application increases the antioxidant activity, and the overall antioxidant effect at high concentration is better than that of comparative example 2, and does not produce cytotoxicity, while comparative example 2 has cytotoxicity.

[0092] The above-mentioned embodiments only express the specific implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the protection scope of the present application. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the technical solution concept of the present application, and these all belong to the protection scope of the present application.

Claims

1. Use of a Chinese medicine composition in the preparation of anti-aging cosmetics, characterized in that: The Chinese medicine composition is prepared from the following raw materials in parts by weight: 15-25 parts of Pseudostellariae Radix, 15-25 parts of Poria, 15-20 parts of Angelicae Sinensis, 15-20 parts of stir-fried Ziziphus jujuba seeds, 10-15 parts of Ophiopogon japonicus, 7-15 parts of roasted Polygala tenuifolia, 5-15 parts of longan pulp, 15-25 parts of Rehmannia root, 15-25 parts of Scrophularia ningpoensis, 10-20 parts of Acorus calamus, 15-25 parts of Platycladus cylindrica seeds, 15-25 parts of roasted Tortoise shell, 15-25 parts of calcined Dragon bone, 6-11 parts of Lotus flower, and 3-11 parts of Licorice root.

2. The use according to claim 1, characterized in that The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 17-23 parts of Pseudostellariae Radix, 17-23 parts of Poria, 17-20 parts of Angelicae Sinensis, 17-20 parts of stir-fried Ziziphus jujuba seeds, 12-15 parts of Ophiopogon japonicus, 9-15 parts of roasted Polygala tenuifolia, 7-13 parts of longan pulp, 17-23 parts of Rehmannia root, 17-23 parts of Scrophularia ningpoensis, 12-18 parts of Acorus calamus, 17-23 parts of Platycladus cylindrica seeds, 17-23 parts of roasted Tortoise shell, 17-23 parts of calcined Dragon bone, 6-9 parts of Lotus, and 3-9 parts of Licorice.

3. The use according to claim 1, characterized in that The lotus is fresh lotus petals picked and freeze-dried for 3-5 days or dried in the shade for 2-4 weeks.

4. The use according to claim 1, characterized in that The traditional Chinese medicine composition is prepared into an external preparation.

5. The use according to claim 4, characterized in that The topical preparation may further include one or more cosmetically acceptable excipients.

6. The use according to claim 1, characterized in that The traditional Chinese medicine composition has the effects of promoting the proliferation of HaCat cells, inducing apoptosis of HaCat senescent cells, improving the antioxidant capacity of HaCat cells, and reducing the expression level of inflammatory factors in HaCat cells.

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