12 results about "Channa argus" patented technology

The invention discloses bacillus subtilis 12-10-2 and application thereof in synergistic preparation of fish oil and fish sauce, and belongs to the technical field of microbial fermentation. The preservation number of the bacillus subtilis (Bacillus subtilis) 12-10-2 is CGMCC (China General Microbiological Culture Collection Center) No.34966. The bacillus subtilis (Bacillus subtilis) 12-10-2 can be used for preparing the bacillus subtilis. The bacillus subtilis 12-10-2 can be used for efficiently hydrolyzing protein in a high-salt environment. The bacillus subtilis 12-10-2 is used for performing bacterium-adding fermentation on the snakehead processing leftovers, so that the fish oil and the fish sauce can be synergistically prepared, and the obtained product is high in yield and good in quality. A new microbial resource is provided for high-value utilization of snakehead processing leftovers, the problems of environmental pollution and resource waste caused by improper treatment can be solved, a new technical support is provided for establishment of a preparation process of high-quality fish oil and fish sauce, and the method has a wide market application prospect.

The invention discloses a method for preventing and treating ichthyophthiriasis of industrially cultured snakehead fries, which belongs to the technical field of snakehead culture and comprises the following steps: completely catching snakehead fries infected with ichthyophthiriasis in a culture pond, and transferring the snakehead fries into a biological bait culture pond for isolated culture; transferring the snakeheads subjected to isolated culture to a culture pond, and raising the salinity of the culture pond for culture; in the same period of culturing in the culture pond, the composite Chinese herbal medicine preparation is splashed into the culture pond to conduct soaking treatment on the snakeheads; after treatment is finished, in daily culture in a culture pond, a red-knees herb decoction water solution is splashed into the culture pond to consolidate the treatment effect of the snakehead and prevent the snakehead. Through the synergistic effect of sick fish isolation, salinity regulation and control and red-knees herb treatment, the survival rate and quality of fish culture are remarkably improved, the culture yield is increased, economic benefits are improved, ecological benefits are improved, and powerful support is provided for green development of factory fish culture.

The invention discloses application of quercetin in preparation of a medicine for preventing and treating iron overload type liver injury and oxidative stress of snakehead, and belongs to the technical field of aquaculture. The invention provides application of quercetin in preparation of a medicine for preventing and treating iron overload type liver injury and oxidative stress of snakehead, and further provides a medicine composition for preventing and treating iron overload type liver injury and oxidative stress of snakehead. Experiments prove that serum AST, ALT, ROS, MDA and 8-OHdG levels and liver iron content of the iron-overload snakehead fed with quercetin are remarkably reduced, non-specific immune indexes LZM, IgM, C3 and C4 activities are remarkably improved, antioxidant ability indexes CAT, SOD, GPx and T-AOC are remarkably improved, the expression levels of antioxidant genes CAT, CU-Zn / SOD, GST and GPx are remarkably increased, and the content of iron in the liver of the snakehead fed with quercetin is remarkably reduced. It is proved that quercetin can be used for preparing the medicine for preventing and treating the snakehead iron overload type liver injury and oxidative stress.

The invention belongs to the technical field of gene engineering, and provides Hsp60 receptor targeted recombinant lactobacillus plantarum as well as a construction method and application thereof, lactobacillus plantarum is taken as a host, pPG612 is taken as an expression vector, and CsgB gene or CsgF gene is expressed. The two engineering probiotics can express target protein and can be stably expressed on the surfaces of thalli, the AB.9 intestinal epithelial cells can be effectively adhered, and the adhesion and invasion of the A.veronii to the AB.9 cells can be inhibited. And secondly, the engineering probiotics LP-pPG-CsgB and LP-pPG-CsgF can promote the proliferation of dendritic cells, T lymphocytes and B lymphocytes, and improve the IgT secretion of intestinal mucus. The engineering bacteria can be competitively combined with an intestinal epithelial cell Hsp60 receptor, down-regulate p65 expression, inhibit NF-kappa B signal channel activation, regulate intestinal tight junction protein redistribution and down-regulate proinflammatory factor expression through a TLR4 / TLR5 dependent NF-kappa B signal channel, and the engineering bacteria can increase the abundance of effective microbial communities in the intestinal tract of the snakeheaded fish, so that the growth of the snakeheaded fish is promoted, and the growth of the snakeheaded fish is promoted. And the flora can transfer, mediate and activate immune response through a feeding environment.

The invention discloses a method for cultivating colored snakeheads. Common white snakehead and golden snakehead in Sichuan and Chongqing regions are used as original parents, reciprocal cross, self-group mating and backcross are sequentially completed to establish a paternal line and a maternal line, then matching hybridization is performed to obtain a G0 generation, then white ground color and colored plaques are used as phenotype markers, and 6-8 generations are continuously bred in combination with growth, low temperature resistance and disease resistance. And finally, obtaining the novel colorful snakehead fish strain with various body colors and stable heredity. Compared with white snakehead, the weight of the novel colorful snakehead strain at the age of 180 days is increased by 20% or above, the low-temperature survival rate is increased by 24% or above, the disease resistance is remarkably enhanced, and the novel colorful snakehead strain is suitable for large-scale culture and ornamental fish markets.

The application belongs to the technical field of biomedicine, and discloses a method for constructing a Channa argus bile stasis model. The application discloses application of rifampicin or a pharmaceutically acceptable salt thereof in construction of a fish liver injury model. The application discloses, for the first time, that the antibiotic rifampicin widely used in aquaculture is used to construct a fish liver injury model. The model is closer to the real scene of the use of a large amount of drugs when bacterial diseases break out in production practice. The injury type of the fish liver injury model is clearly directed to bile stasis, which fills the research blank of the disease model of the fish, and is different from common hepatocyte necrosis or fatty liver models.

The invention belongs to the technical field of biology, discloses a method for regulating the attack behavior of channa argus based on a prolactin PRL signal channel, provides a transcriptional regulation channel of an olfactory prolactin receptor (PRLR) for regulating the attack behavior of channa argus, and finds that a prl gene and caor24 olfactory receptor differential expression are screened from attack difference individuals before and after channa argus breeding. The transcriptional regulation and control effect of the snakehead PRL / PRLR / STAT5 signal channel on caor24 is verified. The method for regulating and controlling the attack behavior of the snakeheaded fish based on the PRL signal channel is established, and a new thought is provided for regulation and control of the attack behavior of the fish. The method provides a practical solution thought for aquaculture attack behavior regulation and control.

The invention discloses a construction method and application of a snakehead iron overload type liver injury model, and belongs to the technical field of animal models. Comprising the following steps: randomly dividing 700-800 snakeheads into four groups, wherein each group comprises six repetitions; feeding the snakehead with feed containing ferrous sulfate heptahydrate with different gradients; the method comprises the following steps: collecting snakehead serum and / or liver samples, carrying out kit determination, Prussian blue staining and Hamp; e, dyeing, observing through a transmission electron microscope and Tunel dyeing, detecting the expression quantity of liver iron transporter genes and proteins, and detecting cell apoptosis; and determining the optimum dosage of the ferrous sulfate heptahydrate induced snakehead liver injury, and repeating the steps to give the optimum dosage of ferrous sulfate heptahydrate induced snakehead liver injury to obtain the snakehead iron overload type liver injury model. The constructed snakehead iron overload type liver injury model is good in standardization and repeatability, can be used for animal model construction or drug screening for treating iron overload type liver diseases, and provides a theoretical basis for deep research of subsequent fish iron overload type liver injury.

The utility model is suitable for the technical field of aquaculture, and provides a comfortable snakehead breeding box which comprises a breeding box body used for snakehead breeding, a partition plate is fixedly installed in the breeding box body, and the breeding box body is divided into two breeding cavities by the partition plate; the mounting frame is fixedly mounted on the breeding box, and a feed box is fixedly mounted on the mounting frame; and the automatic water changing mechanism is arranged on the breeding box and is used for automatically changing water in the breeding box. According to the comfortable snakehead breeding box provided by the scheme, accurate feeding of feed and stable control over the environment are ensured, pollution and waste of water are effectively prevented, and the breeding efficiency and the health level of snakeheads are greatly improved.

The invention belongs to the technical field of attenuated live vaccines, and particularly relates to a snakehead vesicular virus attenuated strain and application thereof. The preservation number of the snakehead vesicular virus attenuated strain is CCTCC (China Center For Type Culture Collection) NO: V202570. The channa argus vesicular virus attenuated strain provided by the invention has the advantages of low toxicity, high biological safety, good immune effect, high protection capacity and the like, can effectively protect fishes from being invaded by channa argus vesicular virus pathogenic strains, and improves the survival rate of infected virulent strains; the immunization can be carried out in manners of intraperitoneal injection, soaking and the like, the immunization manners are flexible and diversified, the operability is high, and the method has good application value and wide market prospect in the field of prevention and control of the vesicular virus diseases of fishes, especially snakeheaded fishes.

The present application relates to a kind of preparation method and application of feed additive for preventing channa maculata deformity, belong to the technical field of feed additive.The present application is mixed, sliced, dried, ground, sieved sequentially to radix astragali, red peony root, salvia miltiorrhiza and bupleurum, to obtain a kind of powder Chinese herbal medicine mixture, then it is added to the water boiling liquid obtained after heating water boiling of pericarpium citri reticulatae, yellowflower and white cardamom by deionized water, and is stirred and mixed, and the bait composed of amino acid, betaine, dimethyl-beta-propionic acid thiothymidine, earthworm powder and plant extract is compounded by temperature rising, finally a kind of feed additive is prepared by high-temperature drying, so that when it is added to channa maculata feed and applied to the daily feeding of channa maculata, on the one hand, it can have excellent effect of preventing channa maculata deformity, on the other hand, it will not adversely affect the taste and palatability of channa maculata feed itself, on the contrary, it can improve the appetite of channa maculata, and the effect of growth and development is better.

The invention relates to an ecological black carp breeding mode for reducing the concentration of pollutants in water of a breeding pond from the source. The ecological black carp breeding mode comprises the following steps: step 1, preparing the breeding pond; 2, cleaning and disinfecting the pond; 3, fry putting, namely putting the fry after disinfection, and carrying out polyculture on black carps, silver carps, bighead carps, snakeheads and hyriopsis cumingii; and 4, feed feeding, wherein the fed feed is black carp functional feed. And 5, pond management. According to the black carp ecological breeding mode for reducing the concentration of the pollutants in the water of the breeding pond from the source, source purification of the pollutants in the breeding water body, improvement of the feed utilization rate, shortening of the growth cycle of the black carps and dual improvement of the yield and the quality of the black carps are effectively achieved, and the remarkable superiority compared with the prior art is highlighted.