24 results about "Channa argus" patented technology

The invention belongs to the technical field of biological medicine, and discloses an application of recombinant protein in preparation of a snakehead vesicular virus vaccine. A G protein of a snakehead vesicular virus (SHVV) is taken as an antigen for the vaccine, recombinant escherichia coli BL21 (pET32a-G) capable of expressing the protein is constructed by utilizing a genetic engineering technical means, recombinant protein rG is obtained through induced expression, and the nano-carrier vaccine (BNC-rG vaccine) of the snakehead vesicular virus is prepared by taking bacterial nano-cellulose as a carrier. According to the BNC-rG vaccine provided by the invention, immune protection against SHVV can be realized through immersion immunization, the specific antibody level is remarkably improved, the relative protection rate of snakehead reaches 80.00% after snakehead is infected with SHVV after immersion immunization for 4 weeks, and the immune protection effect is remarkable.

The invention discloses a primer and probe composition for detecting nocardia seriolae based on TaqMan qPCR (quantitative polymerase chain reaction) and application of the primer and probe composition. According to the invention, a qualitative and quantitative detection method for nocardia seriolae is established by utilizing the specific primers and probes, and the method is used for carrying out qualitative detection on water bodies and fish bodies of farms of snakeheads and hybrid snakeheads in different regions and detecting different tissues after the hybrid snakeheads counteract the nocardia seriolae, and has a very good detection effect. According to the invention, the purposes of nocardia seriolae monitoring, nocardia disease diagnosis, pathological degree measurement and the like can be realized, a reliable detection tool can be provided for nocardia seriolae epidemiological investigation, and nocardia disease can be diagnosed in an early stage so as to guide culture management and reasonable medication treatment; and a research basis and a technical support are provided for exploring epidemic characteristics and pathological mechanisms of nocardia seriolae.

The invention discloses bacillus subtilis 12-10-2 and application thereof in synergistic preparation of fish oil and fish sauce, and belongs to the technical field of microbial fermentation. The preservation number of the bacillus subtilis (Bacillus subtilis) 12-10-2 is CGMCC (China General Microbiological Culture Collection Center) No.34966. The bacillus subtilis (Bacillus subtilis) 12-10-2 can be used for preparing the bacillus subtilis. The bacillus subtilis 12-10-2 can be used for efficiently hydrolyzing protein in a high-salt environment. The bacillus subtilis 12-10-2 is used for performing bacterium-adding fermentation on the snakehead processing leftovers, so that the fish oil and the fish sauce can be synergistically prepared, and the obtained product is high in yield and good in quality. A new microbial resource is provided for high-value utilization of snakehead processing leftovers, the problems of environmental pollution and resource waste caused by improper treatment can be solved, a new technical support is provided for establishment of a preparation process of high-quality fish oil and fish sauce, and the method has a wide market application prospect.

The invention belongs to the technical field of fish immunodetection, and particularly relates to antigenic epitope polypeptide of anti-channa argus IgM, a polyclonal antibody as well as a preparation method and application of the polyclonal antibody. Specifically, the invention successfully constructs a preparation technology system based on the snakehead IgM specific antigen epitope polypeptide and the corresponding polyclonal antibody. Meanwhile, through combination of bioinformatics epitope prediction and immunogenicity optimization, the linear epitope polypeptide with high reactogenicity is successfully screened and obtained. And finally constructing to obtain the anti-channa argus IgM polyclonal antibody, and the verification shows that the polyclonal antibody has high specific recognition capability on channa argus mucus IgM protein. The successful preparation of the antibody fills the blank of a reagent for detecting the mucosal immune globulin of the freshwater economic fish, and provides a core reagent basis for establishing standardized detection platforms such as fish vaccine titer evaluation and immune response dynamic monitoring, so that the antibody has good practical application value.

The invention discloses a method for preventing and treating ichthyophthiriasis of industrially cultured snakehead fries, which belongs to the technical field of snakehead culture and comprises the following steps: completely catching snakehead fries infected with ichthyophthiriasis in a culture pond, and transferring the snakehead fries into a biological bait culture pond for isolated culture; transferring the snakeheads subjected to isolated culture to a culture pond, and raising the salinity of the culture pond for culture; in the same period of culturing in the culture pond, the composite Chinese herbal medicine preparation is splashed into the culture pond to conduct soaking treatment on the snakeheads; after treatment is finished, in daily culture in a culture pond, a red-knees herb decoction water solution is splashed into the culture pond to consolidate the treatment effect of the snakehead and prevent the snakehead. Through the synergistic effect of sick fish isolation, salinity regulation and control and red-knees herb treatment, the survival rate and quality of fish culture are remarkably improved, the culture yield is increased, economic benefits are improved, ecological benefits are improved, and powerful support is provided for green development of factory fish culture.

The utility model discloses a lunar snakehead pollution discharge collecting device, which relates to the technical field of fishery breeding pollution discharge and comprises a collecting filter bin, the outer surface of the front end of the collecting filter bin is detachably connected with a waterproof motor, the outer wall of the collecting filter bin is provided with a filter frame, gas storage tanks and an inclined guide pipe, and the gas storage tanks are positioned on two sides of the filter frame. According to the pollution discharge and collection device for the channa maculata, the arranged water pump can pump pond water into the collection filter bin during use, then dirt in the pond water can be blocked and filtered by the filter plate, and the filtered pond water can be filtered again through the filter frame and is discharged through the discharge pipe orifice to flow back into a pond; the waterproof motor can drive the middle shaft rod during use, the middle shaft rod can drive the filter plate to move by a small angle to swing in a reciprocating mode, filtered dirt can be guided into the inclined guide pipe and guided into the dirt collecting tank through the inclined guide pipe to be stored, the dirt collecting efficiency is high during use, and the dirt can be conveniently collected and stored.

The invention discloses application of quercetin in preparation of a medicine for preventing and treating iron overload type liver injury and oxidative stress of snakehead, and belongs to the technical field of aquaculture. The invention provides application of quercetin in preparation of a medicine for preventing and treating iron overload type liver injury and oxidative stress of snakehead, and further provides a medicine composition for preventing and treating iron overload type liver injury and oxidative stress of snakehead. Experiments prove that serum AST, ALT, ROS, MDA and 8-OHdG levels and liver iron content of the iron-overload snakehead fed with quercetin are remarkably reduced, non-specific immune indexes LZM, IgM, C3 and C4 activities are remarkably improved, antioxidant ability indexes CAT, SOD, GPx and T-AOC are remarkably improved, the expression levels of antioxidant genes CAT, CU-Zn / SOD, GST and GPx are remarkably increased, and the content of iron in the liver of the snakehead fed with quercetin is remarkably reduced. It is proved that quercetin can be used for preparing the medicine for preventing and treating the snakehead iron overload type liver injury and oxidative stress.

The invention belongs to the technical field of gene engineering, and provides Hsp60 receptor targeted recombinant lactobacillus plantarum as well as a construction method and application thereof, lactobacillus plantarum is taken as a host, pPG612 is taken as an expression vector, and CsgB gene or CsgF gene is expressed. The two engineering probiotics can express target protein and can be stably expressed on the surfaces of thalli, the AB.9 intestinal epithelial cells can be effectively adhered, and the adhesion and invasion of the A.veronii to the AB.9 cells can be inhibited. And secondly, the engineering probiotics LP-pPG-CsgB and LP-pPG-CsgF can promote the proliferation of dendritic cells, T lymphocytes and B lymphocytes, and improve the IgT secretion of intestinal mucus. The engineering bacteria can be competitively combined with an intestinal epithelial cell Hsp60 receptor, down-regulate p65 expression, inhibit NF-kappa B signal channel activation, regulate intestinal tight junction protein redistribution and down-regulate proinflammatory factor expression through a TLR4 / TLR5 dependent NF-kappa B signal channel, and the engineering bacteria can increase the abundance of effective microbial communities in the intestinal tract of the snakeheaded fish, so that the growth of the snakeheaded fish is promoted, and the growth of the snakeheaded fish is promoted. And the flora can transfer, mediate and activate immune response through a feeding environment.

The invention discloses a method for cultivating colored snakeheads. Common white snakehead and golden snakehead in Sichuan and Chongqing regions are used as original parents, reciprocal cross, self-group mating and backcross are sequentially completed to establish a paternal line and a maternal line, then matching hybridization is performed to obtain a G0 generation, then white ground color and colored plaques are used as phenotype markers, and 6-8 generations are continuously bred in combination with growth, low temperature resistance and disease resistance. And finally, obtaining the novel colorful snakehead fish strain with various body colors and stable heredity. Compared with white snakehead, the weight of the novel colorful snakehead strain at the age of 180 days is increased by 20% or above, the low-temperature survival rate is increased by 24% or above, the disease resistance is remarkably enhanced, and the novel colorful snakehead strain is suitable for large-scale culture and ornamental fish markets.

The present invention belongs to the field of biotechnology. The present invention discloses a molecular marker closely linked to the sex gene of Channa argus, which can accurately identify the genetic sex of Channa argus. The present invention also provides a specific KASP primer for amplifying the molecular marker closely linked to the sex gene of Channa argus, which can detect the genetic sex of Channa argus, is simple and easy to use, has a high analysis throughput, high accuracy, and is suitable for the detection of a large number of samples. The molecular marker provided by the present invention has important practical significance for quickly and accurately screening pseudo-female Channa argus and super-male Channa argus, shortening the breeding time, and improving the breeding efficiency.

The application belongs to the technical field of biomedicine, and discloses a method for constructing a Channa argus bile stasis model. The application discloses application of rifampicin or a pharmaceutically acceptable salt thereof in construction of a fish liver injury model. The application discloses, for the first time, that the antibiotic rifampicin widely used in aquaculture is used to construct a fish liver injury model. The model is closer to the real scene of the use of a large amount of drugs when bacterial diseases break out in production practice. The injury type of the fish liver injury model is clearly directed to bile stasis, which fills the research blank of the disease model of the fish, and is different from common hepatocyte necrosis or fatty liver models.

The invention discloses a coloration LAMP (loop-mediated isothermal amplification) rapid detection method for nocardiasis of snakehead. The method comprises the following steps: (1) extracting a to-be-detected snakehead tissue DNA (deoxyribonucleic acid) sample; (2) adding the DNA sample into an LAMP reaction system, wherein the reaction system comprises a specific primer group, Bst DNA polymerase, dNTPs, a reaction buffer solution and a color indicator hydroxycalcein, wherein the specific primer group is designed aiming at a nocardia argus 16S rRNA gene conserved region; (3) reacting for 20 minutes at the constant temperature of 65 DEG C in 25 microliters of sample adding system; and (4) after the reaction is finished, if nocardia argus exists in the sample, the reaction liquid becomes green, and if nocardia argus does not exist, the reaction liquid becomes orange. The method is simple and convenient to operate and suitable for convenient and rapid detection in production. The whole LAMP detection time is shortened to 30 minutes, and the detection can be completed; compared with a commercially available kit, the speed is increased by 41.59%; the primers are high in specificity, the sensitivity of the primers is 10-100 times higher than that of conventional PCR, low-concentration pathogenic bacteria can be effectively detected, and the primers are suitable for on-site rapid detection and large-scale screening in nocardiasis of channa argus and fry production.

The invention belongs to the technical field of molecular biology, discloses an SNP molecular marker closely linked with genetic sex of channa maculata and application of the SNP molecular marker, and particularly discloses the SNP molecular marker closely linked with genetic sex of channa maculata, the sequence of the SNP molecular marker is as shown in SEQ ID NO: 1, the SNP site is located at the 151st site from the 5'terminal of the sequence as shown in SEQ ID NO: 1, and the basic group is T / G. The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker closely linked with an ophicephalus obscura sex gene. The genetic sex of the ophicephalus obscura can be accurately identified through the single base difference in the molecular marker. The invention also provides a specific KASP primer for amplifying the molecular marker closely linked with the sex gene of the snakehead, which can be used for detecting the genetic sex of the snakehead, is simple and easy to use, has high analysis flux and high accuracy, and is suitable for detecting a large number of samples.

The invention discloses a strain of spermaceti soxhlet ZX 2401Ca, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 9, 2025, and the preservation number is CGMCC No.33340. The invention also discloses a method for preparing the spermaceti soxhlet ZX 2401Ca. The cetella sobolifera ZX 2401Ca is indigenous bacteria in the intestinal tract of the snakeheaded fish, and is obtained by screening from the intestinal tract of the snakeheaded fish for the first time. The strain can be metabolized to generate short-chain fatty acid mainly comprising acetic acid, and has very strong oxidation resistance. According to breeding experiments, the inventor finds that the fermented feed prepared from the strain has the functions of improving antioxidant capacity disorder, hepatopancreas injury and the like caused by oxidized fish oil, and also has a good effect in the aspect of improving the aeromonas hydrophila infection resistance of macrobrachium rosenbergii.

The invention belongs to the technical field of biology, discloses a method for regulating the attack behavior of channa argus based on a prolactin PRL signal channel, provides a transcriptional regulation channel of an olfactory prolactin receptor (PRLR) for regulating the attack behavior of channa argus, and finds that a prl gene and caor24 olfactory receptor differential expression are screened from attack difference individuals before and after channa argus breeding. The transcriptional regulation and control effect of the snakehead PRL / PRLR / STAT5 signal channel on caor24 is verified. The method for regulating and controlling the attack behavior of the snakeheaded fish based on the PRL signal channel is established, and a new thought is provided for regulation and control of the attack behavior of the fish. The method provides a practical solution thought for aquaculture attack behavior regulation and control.

The invention discloses a method for discriminating the quality of snakehead fry, and belongs to the field of aquaculture. According to the method, the behavioral parameters such as average speed, acceleration and motion trail uniformity of the snakehead fry are selected as fry quality correlation indexes, so that the quality of the snakehead fry is accurately judged. By means of the intelligent means, the detection speed of 2 minutes per batch is achieved, meanwhile, fry damage is reduced by 85.6%, early intervention of diseases can be achieved according to the recognition result, therefore, the efficiency and accuracy of snakehead fry quality judgment are improved, errors and damage caused by manual operation are reduced, and important application value and popularization prospects are achieved.

The utility model relates to the technical field of aquaculture, in particular to an assembled snakehead spawning, hatching and breeding device. The aquaculture net cage comprises a plurality of aquaculture net cage units, and each aquaculture net cage unit is arranged in a cuboid / cube shape and comprises a bottom frame, a net cage, a top cover and a zipper. The bottom frame is of a hollow tubular structure, gravel for counterweight is filled in the bottom frame, the net cage is of a cylindrical structure formed by sewing 40-60-mesh screen cloth, a zipper is arranged on the periphery of the top edge of the net cage, and the top cover is fixed above the net cage through the zipper; the adjacent aquaculture net cage units are assembled through zippers, clamping joints are arranged at the four end points of the assembled aquaculture net cage units, and nylon ropes penetrate through the clamping joints to enable the aquaculture net cage units to be hung and fixed. By means of the modularized culture net cage units, efficient utilization of water resources and flexible management of the culture process are achieved, the culture efficiency is improved through the overall structure, and the air permeability of the culture environment and the water quality stability are guaranteed.

The invention discloses a construction method and application of a snakehead iron overload type liver injury model, and belongs to the technical field of animal models. Comprising the following steps: randomly dividing 700-800 snakeheads into four groups, wherein each group comprises six repetitions; feeding the snakehead with feed containing ferrous sulfate heptahydrate with different gradients; the method comprises the following steps: collecting snakehead serum and / or liver samples, carrying out kit determination, Prussian blue staining and Hamp; e, dyeing, observing through a transmission electron microscope and Tunel dyeing, detecting the expression quantity of liver iron transporter genes and proteins, and detecting cell apoptosis; and determining the optimum dosage of the ferrous sulfate heptahydrate induced snakehead liver injury, and repeating the steps to give the optimum dosage of ferrous sulfate heptahydrate induced snakehead liver injury to obtain the snakehead iron overload type liver injury model. The constructed snakehead iron overload type liver injury model is good in standardization and repeatability, can be used for animal model construction or drug screening for treating iron overload type liver diseases, and provides a theoretical basis for deep research of subsequent fish iron overload type liver injury.

The utility model is suitable for the technical field of aquaculture, and provides a comfortable snakehead breeding box which comprises a breeding box body used for snakehead breeding, a partition plate is fixedly installed in the breeding box body, and the breeding box body is divided into two breeding cavities by the partition plate; the mounting frame is fixedly mounted on the breeding box, and a feed box is fixedly mounted on the mounting frame; and the automatic water changing mechanism is arranged on the breeding box and is used for automatically changing water in the breeding box. According to the comfortable snakehead breeding box provided by the scheme, accurate feeding of feed and stable control over the environment are ensured, pollution and waste of water are effectively prevented, and the breeding efficiency and the health level of snakeheads are greatly improved.

The utility model discloses an automatic screening snakehead breeding device, and belongs to the technical field of snakehead breeding. The automatic screening snakehead breeding equipment comprises a breeding box body, a water inlet pipe, a water outlet pipe and an air guide assembly, wherein the water inlet pipe, the water outlet pipe and the air guide assembly are arranged on the breeding box body; at least one screening plate is vertically arranged in the breeding box body, the screening plate extends into the breeding box body from the top of the breeding box body and is detachably connected with the breeding box body, a screen is arranged on the screening plate, an inner cavity of the breeding box body is divided into a plurality of breeding cavities by all the screening plates, and the breeding cavities are communicated with the air guide assembly. According to the automatic screening snakehead breeding equipment, the inner cavity of the breeding box body is divided into a plurality of breeding cavities through the screening plates, the screens on the screening plates can automatically screen snakeheads according to the size, the situation that big fishes eat small fishes is avoided, the survival rate of the snakeheads is increased, and therefore the breeding rate of the snakeheads is increased; and the device also has the functions of automatic water change and aeration oxygen supply.

The invention belongs to the technical field of attenuated live vaccines, and particularly relates to a snakehead vesicular virus attenuated strain and application thereof. The preservation number of the snakehead vesicular virus attenuated strain is CCTCC (China Center For Type Culture Collection) NO: V202570. The channa argus vesicular virus attenuated strain provided by the invention has the advantages of low toxicity, high biological safety, good immune effect, high protection capacity and the like, can effectively protect fishes from being invaded by channa argus vesicular virus pathogenic strains, and improves the survival rate of infected virulent strains; the immunization can be carried out in manners of intraperitoneal injection, soaking and the like, the immunization manners are flexible and diversified, the operability is high, and the method has good application value and wide market prospect in the field of prevention and control of the vesicular virus diseases of fishes, especially snakeheaded fishes.

The present application relates to a kind of preparation method and application of feed additive for preventing channa maculata deformity, belong to the technical field of feed additive.The present application is mixed, sliced, dried, ground, sieved sequentially to radix astragali, red peony root, salvia miltiorrhiza and bupleurum, to obtain a kind of powder Chinese herbal medicine mixture, then it is added to the water boiling liquid obtained after heating water boiling of pericarpium citri reticulatae, yellowflower and white cardamom by deionized water, and is stirred and mixed, and the bait composed of amino acid, betaine, dimethyl-beta-propionic acid thiothymidine, earthworm powder and plant extract is compounded by temperature rising, finally a kind of feed additive is prepared by high-temperature drying, so that when it is added to channa maculata feed and applied to the daily feeding of channa maculata, on the one hand, it can have excellent effect of preventing channa maculata deformity, on the other hand, it will not adversely affect the taste and palatability of channa maculata feed itself, on the contrary, it can improve the appetite of channa maculata, and the effect of growth and development is better.

The invention relates to a compound preparation suitable for breeding hybrid snakehead in a high-temperature period and a preparation method, and belongs to the technical field of fish breeding. The invention provides a preparation method of a compound preparation. The preparation method comprises the following steps: mixing fermentation liquor and compound probiotics, stirring, freeze-drying the obtained mixture to obtain a dried product, putting the dried product into a spray dryer, spraying outer layer liquid on the surface of the dried product, and drying to obtain the compound preparation. The compound preparation is prepared by wrapping the content with the outer-layer liquid, the outer-layer liquid is obtained by compounding the pectin solution and the concentrated solution, the content is obtained by compounding the compound probiotics and the fermentation liquor, and beneficial components in the content are released along with gradual disappearance of the outer-layer liquid, so that the compound preparation is free of side effects. The beneficial components not only can provide comprehensive nutrition for the hybrid snakehead, but also can improve the absorption and conversion capability of the hybrid snakehead to the feed in the high-temperature period, provide guarantee for the intestinal health of the hybrid snakehead, and further promote the growth and development of the hybrid snakehead.

The invention relates to an ecological black carp breeding mode for reducing the concentration of pollutants in water of a breeding pond from the source. The ecological black carp breeding mode comprises the following steps: step 1, preparing the breeding pond; 2, cleaning and disinfecting the pond; 3, fry putting, namely putting the fry after disinfection, and carrying out polyculture on black carps, silver carps, bighead carps, snakeheads and hyriopsis cumingii; and 4, feed feeding, wherein the fed feed is black carp functional feed. And 5, pond management. According to the black carp ecological breeding mode for reducing the concentration of the pollutants in the water of the breeding pond from the source, source purification of the pollutants in the breeding water body, improvement of the feed utilization rate, shortening of the growth cycle of the black carps and dual improvement of the yield and the quality of the black carps are effectively achieved, and the remarkable superiority compared with the prior art is highlighted.