Anti-channa argus IgM antigen epitope polypeptide, polyclonal antibody as well as preparation method and application of anti-channa argus IgM antigen epitope polypeptide and polyclonal antibody

Through in-depth research on blackfish mucus IgM, anti-black mucus IgM polyclonal antibodies were prepared, which solved the gap in the study of blackfish IgM antibodies in freshwater economic fish, and provided core reagents for fish vaccine titer evaluation and dynamic monitoring of immune responses.

CN119841946APending Publication Date: 2025-04-18FRESHWATER FISHERIES RES INST OF SHANDONG PROVINCE
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Patent Information

Application Number
CN202510161061.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-13
Publication Date
2025-04-18

AI Technical Summary

Technical Problem

There is a gap in the study of immunoglobulin antibodies in freshwater economic fish, especially mucus IgM antibodies in black birds, which has not yet been established for the correlation between its immune response characteristics and vaccine protective titers.

Method used

Through in-depth research on black veal mucus IgM, its antigen epitope polypeptide was obtained, and this was used as an antigen to black veal mucus IgM polyclonal antibody was successfully prepared.

Benefits of technology

A technical system for the preparation of IgM-specific antigen epitope polypeptides and corresponding polyclonal antibodies was successfully constructed, filling the gap in freshwater economic fish mucosal immunoglobulin detection reagents, and providing a core reagent basis for establishing a standardized detection platform for fish vaccine titer evaluation and dynamic monitoring of immune responses.

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Abstract

The invention belongs to the technical field of fish immunodetection, and particularly relates to antigenic epitope polypeptide of anti-channa argus IgM, a polyclonal antibody as well as a preparation method and application of the polyclonal antibody. Specifically, the invention successfully constructs a preparation technology system based on the snakehead IgM specific antigen epitope polypeptide and the corresponding polyclonal antibody. Meanwhile, through combination of bioinformatics epitope prediction and immunogenicity optimization, the linear epitope polypeptide with high reactogenicity is successfully screened and obtained. And finally constructing to obtain the anti-channa argus IgM polyclonal antibody, and the verification shows that the polyclonal antibody has high specific recognition capability on channa argus mucus IgM protein. The successful preparation of the antibody fills the blank of a reagent for detecting the mucosal immune globulin of the freshwater economic fish, and provides a core reagent basis for establishing standardized detection platforms such as fish vaccine titer evaluation and immune response dynamic monitoring, so that the antibody has good practical application value.
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