689 results about "Immuno detection" patented technology

The invention discloses an intestinal cancer immune response graph neural network prediction method, a medium and equipment, and the method comprises the steps: collecting pathological image information, immunodetection information and basic clinical information, extracting a tissue space distribution characteristic spectrum through a deep convolutional network, and constructing a graph neural network model in combination with an immunomarker expression characteristic matrix; spatial interaction characteristics of a tumor microenvironment are modeled by adopting a graph attention mechanism, finally a treatment response probability, an optimal treatment opportunity and an adverse reaction risk are predicted through a multi-task learning framework, and a clinical decision report containing a prediction response curve, a risk early warning threshold and a treatment time window suggestion is output. According to the method, through multi-modal data fusion and spatial interaction modeling, accurate prediction of intestinal cancer immunotherapy response is realized, and a more comprehensive reference basis is provided for clinical decision making.

The utility model relates to the field of microfluidic detection and fluorescence immunoassay, in particular to a portable biochemical blood coagulation and fluorescence immunoassay integrated analysis device, which comprises a shell, a power supply arranged in the shell, two support plates arranged in the shell, a fluorescent bracket fixedly connected to the upper ends of the support plates, a support arranged in the shell, a lamp source arranged at the upper end of the support, and a light source arranged at the lower end of the lamp source. A microfluidic detection module is mounted in the support, a fluorescence immunodetection module is mounted at the upper end of the fluorescence bracket, an optical module is arranged in the fluorescence immunodetection module, and a bar code scanner for confirming information of a detection chip or a reagent strip is arranged on one side of the optical module; compared with the traditional detection equipment with a single function, the double detection functions of biochemistry and fluorescence immunity are realized, the detection efficiency and comprehensiveness are greatly improved, meanwhile, the kit also supports the simultaneous detection of three reagent strips with different specifications, and the flexibility and convenience of the detection are further enhanced.

The invention provides an immunodetection method of a multi-index immune checkpoint detection kit, which belongs to the technical field of immunodetection and comprises three main stages of sample preparation, immune reaction and data analysis. The method comprises the following steps: firstly, carrying out centrifugal separation to obtain a plasma sample, and preparing a fluorescent microsphere coupled capture antibody and biotin labeled detection antibody compound; then carrying out immune reaction, forming a sandwich type immune complex through specific binding, and adding streptavidin marked by phycoerythrin for signal amplification. Detecting a fluorescence signal by adopting a flow cytometer to obtain an original data matrix; a contribution feature vector, a utility feature vector and an expression feature matrix are extracted through a feature decomposition algorithm, and a deep learning model of a total-score-total structure is established for data analysis. Finally, a detection report containing a quantitative result, an importance score and reliability evaluation is generated, and the technical problem that in the prior art, the analysis accuracy of a multi-index immune checkpoint detection result is insufficient is solved.

The invention belongs to the technical field of immunodetection, and discloses a kit for detecting the ratio of proBDNF to maure BDNF based on structural dynamics guidance and a preparation method of the kit, the kit comprises a solid phase carrier and a detection reagent, the three antibodies are determined on the basis of full-length conformational kinetics analysis of brain-derived neurotrophic factors; the first capture antibody targets an innate disorder region (SEQ ID NO: 1) of a proBDNF propeptide region; the second capture antibody targets an innate disorder region (SEQ ID NO: 2) of a proBDNF propeptide region; the second capture antibody is a neo-epitope specific antibody, is specifically combined with an N terminal (SEQ ID NO: 2) exposed by maure BDNF enzyme digestion, and is combined with a free alpha-amino group strictly dependent on the first histidine; and the universal detection antibody is combined to a Loop 4 region (SEQ ID NO: 3) of the Mature structural domain. The problems of steric hindrance and cross reaction in traditional immunodetection are solved through a structural biology strategy, and accurate distinguishing and ratio quantification of proBDNF and maure BDNF are achieved.

The invention relates to a hybridoma cell strain capable of secreting a mefos monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. According to the invention, the hybridoma cell strain capable of secreting the fenofos monoclonal antibody is obtained through screening, the fenofos monoclonal antibody has high specificity and high sensitivity to the fenofos, IC50 to the fenofos is 0.231 ng / mL, and the fenofos monoclonal antibody has no cross reaction to structural analogues of the fenofos, such as triazophos, diazinon, phorate, acetyl phorate, methamidophos and the like; therefore, low-concentration isoprophos can be accurately detected.

The invention discloses an enzyme linked immunosorbent assay kit for detecting a plasticizer in edible oil and application of the enzyme linked immunosorbent assay kit, and belongs to the technical field of enzyme linked immunosorbent assay. The enzyme linked immunosorbent assay kit comprises an elisa plate coated with a plasticizer coupling antigen, a plasticizer standard substance, an enzyme conjugate working solution, a plasticizer specific antibody working solution, a purification tube, a purification reagent, a standard substance diluent, a sample diluent, a substrate solution, a stop solution and a 10 * concentrated washing solution. The standard substance diluent provided by the invention is used for diluting a plasticizer standard substance at different temperatures to obtain a plasticizer standard solution, and the obtained plasticizer standard solution has basically consistent inhibition ratio, so that the stability of the kit is remarkably enhanced; meanwhile, the standard diluent and other components in the kit have a synergistic effect, so that the enzyme linked immunosorbent assay kit has the advantages of good specificity, high sensitivity, good accuracy and high stability when being used for detecting the plasticizer in the edible oil; therefore, the enzyme linked immunosorbent assay kit has a good application prospect in the detection of the plasticizer in the edible oil.

The invention relates to a hybridoma cell strain capable of secreting dextromethorphan monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. The monoclonal antibody secreted by the hybridoma cell strain disclosed by the invention has good sensitivity and specificity on dextromethorphan, wherein the IC50 value on the dextromethorphan is 0.162 ng / mL. The monoclonal antibody provided by the invention has no cross reaction on structural analogues of dextromethorphan, such as fentanyl, thilazine, papaverine, morphine and medetomidine, and has good specificity, so that the dextromethorphan with low concentration can be accurately detected.

The invention provides a monoclonal antibody 34C1 for resisting vitamin B12. The sequence of a heavy chain CDR1 of the monoclonal antibody 34C1 is as shown in SEQ ID NO: 1, the sequence of CDR2 of the monoclonal antibody 34C1 is as shown in SEQ ID NO: 2, and the sequence of CDR3 of the monoclonal antibody 34C1 is as shown in SEQ ID NO: 3; the sequence of the light chain CDR1 is shown as SEQ ID NO: 4, the sequence of the CDR2 is shown as SEQ ID NO: 5, and the sequence of the CDR3 is shown as SEQ ID NO: 6. The antibody can specifically recognize VB12, and can be developed into an immunodetection reagent based on a competition law principle or a sandwich law principle.

The invention relates to the technical field of enzyme-linked immunosorbent assay, and discloses a quality control serum diluent as well as a preparation method and application thereof, and the quality control serum diluent is prepared from the following raw materials: boric acid, borax, sodium caseinate, liquid gelatin, aminopyrine, an osmotic pressure stabilizer and bovine serum. The quality control serum diluent prepared by adopting the formula has no potential interfering substances (such as formaldehyde and thiomersalate), has excellent thermal stability and long-term stability, is applied to quality control serum dilution, is beneficial to improving the detection accuracy, ensures that the characteristics of the diluent meet the requirements when the diluent is used every time, and provides stable reference data for the detection process.

The invention relates to a medetomidine hapten, a monoclonal antibody, a hybridoma cell strain and application thereof, and belongs to the technical field of immunodetection. A medetomidine complete antigen is obtained by synthesizing a medetomidine hapten, a mouse is used as an immune animal to prepare a hybridoma cell strain secreting a medetomidine monoclonal antibody, the hybridoma cell strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.46518. The medetomidine monoclonal antibody secreted by the hybridoma cell strain has better detection sensitivity and detection specificity to medetomidine, the IC50 value is 10.42 ng / mL, and the cross reaction rate to various medetomidine analogues is lt; the method can be applied to preparation of medetomidine detection products, and provides a reliable tool for immunodetection of medetomidine.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for detecting feline coronavirus NP protein and application of the monoclonal antibody combination. The combination is composed of monoclonal antibodies 3C6 and 6H9, 3C6 serves as a coating antibody, 6H9 is used for colloidal gold labeling, and efficient and specific sandwich detection of feline coronavirus NP protein can be achieved. According to the invention, the monoclonal antibody combination is correspondingly applied to an immunodetection platform, and a rapid detection test strip or test paper card based on a colloidal gold immunochromatography technology is constructed. The test paper has high sensitivity to FECV-NP and FIBV-NP recombinant proteins, has no cross reaction with other proteins, and is suitable for rapid and on-site detection.

The invention discloses a liquid cell product, a preparation method thereof and a preserving fluid for preparing the liquid cell product, and belongs to the technical field of immunodetection. The dendritic polymer is creatively added into the preserving fluid for preparing the liquid cell product, the cell morphology in the liquid cell product can be improved, and a thermal acceleration test proves that the preserving fluid can maintain the cell morphology in the liquid cell product for a long time. When the preservation solution is used for preparing the liquid cell quality control product for immunodetection, quality control spots with complete circles can be formed after sample application on slides made of different materials, it can be obviously judged that cells in the liquid cell quality control product are dispersed very uniformly after immunohistochemical staining, and the preservation solution has very high application value.

The invention provides application of high-concentration colloidal gold in preparation of an influenza virus detection test strip, and relates to the technical field of immunodetection. The detection test strip provided by the invention is composed of a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a bottom plate, and in the preparation process of the detection test strip, high-concentration colloidal gold with the OD value of 100 is adopted for respectively marking an influenza A virus monoclonal antibody 1, an influenza B virus monoclonal antibody 1 and a chicken IgY antibody; optimized colloidal gold labeled reconstitution fluid is mixed and then sprayed on the combination pad, and meanwhile, a corresponding capture antibody and a goat anti-chicken IgY antibody are respectively sprayed on the nitrocellulose membrane to form a detection line and a quality control line. Through the synergistic effect of the high-concentration colloidal gold mark, the optimized sample pad / combination pad treating fluid and the special sample diluent, the detection sensitivity is remarkably improved, the false negative rate is effectively reduced, and rapid, high-sensitivity and high-specificity field detection on influenza A and B viruses is realized.

The invention discloses a coal mill internal multi-source heterogeneous data fusion early warning method and system, and belongs to the technical field of industrial equipment fault diagnosis. The method aims at solving the technical problems that an existing coal mill early warning method is insufficient in multi-source data fusion, poor in working condition adaptability, low in early warning accuracy and lack of fault tracing and accurate control. According to the technical scheme, the method comprises the steps that structured process parameter data and unstructured physical field data of the coal mill are obtained; constructing a digital antigen vector representing a running state; constructing an autologous space based on the historical health data, and generating a dynamic immunodetector set covering a non-autologous space; calculating the Euclidean distance between the digital antigen vector and the detector and a danger signal index; early warning is triggered through cooperative judgment, feedback control is executed, and a fault source is positioned in combination with fault tracing. The device state can be comprehensively represented, the working condition adaptability and the early warning accuracy are improved, accurate fault positioning and control are achieved, and the device maintenance cost is reduced.

The invention provides a method for analyzing interaction between procambarus clarkia arginine kinase and shark source nano antibody. The method comprises the following steps: performing three-dimensional structure prediction on a VNAR sequence, and screening a high-confidence VNAR model by using a Laplace map; docking an amino acid sequence corresponding to the VNAR model with an arginine kinase sequence to obtain an optimal compound model, and analyzing an interaction site of an arginine kinase epitope region and a nanometer antibody complementary determining region CDR3; performing molecular dynamics simulation on the compound model, calculating conformational change indexes and binding free energy through energy optimization, system balance and extended simulation sampling, and outputting a binding stability sequence of the nano antibody and arginine kinase; the method comprises the following steps: screening out a high-stability nano antibody, pre-incubating a fusion protein of the high-stability nano antibody and immobilized arginine kinase to form a compound, adding serum of an allergic patient for competitive binding, quantifying the binding activity of residual IgE through an immunodetection technology, and verifying the inhibition effect of the shark source nano antibody on the sensitization effect of arginine kinase.

The invention discloses a preparation method and application of an HPV16 / 18 type E6 / E7 protein multi-target detection test strip, and belongs to the technical field of biological immunodetection analysis. The detection test strip comprises a back plate, a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad, AIE fluorescent microsphere labeled probes are distributed on the combination pad, and a capture antibody and an AIE solution are distributed on the nitrocellulose membrane. The preparation method comprises the following three steps: preparation of the nitrocellulose membrane, preparation of the combination pad and assembly of the test strip. The detection test strip can synchronously and quantitatively detect PV16 E6, HPV16 E7, HPV18 E6 and HPV18 E7, each target corresponds to four signal points, multi-target synchronous rapid detection is achieved, the linear range is wide, the detection limit is low, operation is easy and convenient, cost is low, and the detection test strip is expected to become a novel efficient and reliable on-site screening tool for cervical cancer and precancerous lesions.

The utility model discloses automatic enzyme-linked immunoassay detection equipment which comprises a processing module and a washing liquid module, and the processing module comprises a pipetting module, a clamping jaw module and an operation processing area, the operation treatment area is provided with a reaction container, a cleaning position, a cleaning head matched with the cleaning position, an oscillation incubation area, a sample adding area, a consumable loading area, a reagent loading area and an enzyme immunoassay detection area, the cleaning head comprises a liquid injection needle and a liquid suction needle, and the pipetting module and the clamping jaw module can be driven to move along the operation treatment area. The pipetting module can transfer a sample liquid or a reagent to the reaction container, the clamping jaw module can move to the reaction container to perform cover opening and closing operation and can transfer the reaction container into a cleaning position, and the washing liquid module can be in fluid communication with the liquid injection needle and the liquid suction needle to complete cleaning of the reaction container. The detection equipment provided by the utility model can realize a series of full-automatic enzyme immunoassay detection such as sample transfer, reagent transfer, enzyme immunoassay reaction, cleaning and enzyme immunoassay detection.

The invention relates to the technical field of biomedicine, in particular to an anti-human immunodeficiency virus type 1 p24 and type 2 p26 antibody and application thereof. The invention provides an antibody or an antigen-binding fragment thereof capable of specifically binding to a p24 protein of human immunodeficiency virus type 1 and a p26 protein of human immunodeficiency virus type 2, and also provides a combination of antibody pairs, a kit and related applications. When the antibody pair is used for immunodetection, antigens of HIV-1 type p24 and HIV-2 type p26 can be detected at the same time, and the antibody pair has the advantage of lower concentration detection limit.

The invention belongs to the technical field of immunodetection, and discloses an avian single-chain antibody for detecting a liver cancer marker GPC3, a kit and a detection method. The poultry-derived single-chain antibody has the characteristics of small volume, high specificity, high stability and good sensitivity, and can be used in an immunoassay method for detecting a liver cancer marker GPC3; according to the method, a chicken-derived single-chain antibody is coated on a solid-phase carrier, a rabbit-derived polyclonal antibody is taken as a detection carrier, the antibodies are combined with a GPC3 antigen to form a sandwich structure, and the double-antibody sandwich immunoassay method of the GPC3 is established by using the method, and has the advantages of high specificity, good sensitivity, high sensitivity and high sensitivity. The invention provides a rapid, accurate and reliable detection method for detecting GPC3 in serum.

The invention provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody as well as a hybridoma cell strain and application thereof, and belongs to the technical field of immune globulin. The invention provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody and also provides a hybridoma cell strain OTI10F11, the hybridoma cell strain OTI10F11 is preserved in the China General Microbiological Culture Collection Center on April 26, 2025, and the preservation number of the hybridoma cell strain OTI10F11 is No.46356. The invention further provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody. The hybridoma cell strain OTI10F11 provided by the invention can stably secrete an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody, and can be specifically combined with H < + > / K < + > ATPase beta protein, so that the specificity, accuracy and reliability of immunodetection are remarkably improved, and the hybridoma cell strain OTI10F11 can be suitable for marking H < + > / K < + > ATPase beta protein in tissue cells.

The invention belongs to the technical field of immunodetection, and discloses a preparation and characterization method of a rabbit-derived single-chain antibody for detecting a liver cancer marker GPC3. The rabbit-derived single-chain antibody has the characteristics of small volume, high specificity, high stability and good sensitivity, and can be used in an immunoassay method for detecting a liver cancer marker GPC3; according to the method, a specific rabbit single-chain antibody is obtained through screening, expression and purification by using a phage display technology, a GPC3 antigen is coated on a solid-phase carrier, the rabbit single-chain antibody is used as a detection carrier, the binding force of the single-chain antibody and the antigen is identified, and the method is high in specificity, good in sensitivity and high in specificity. A novel single-chain antibody with high specificity and high sensitivity is provided for detection of GPC3 in serum.

The invention discloses a biochemical immunodetection device and a biochemical immunodetection method for ursodesoxycholic acid reduzate, and belongs to the technical field of ursodesoxycholic acid reduzate detection. The ursodesoxycholic acid reduzate detection device comprises a detection box and a lifter at the bottom of the detection box, a dissolution preparation part and a centrifugal part are arranged in the detection box, and the dissolution preparation part can fully dissolve an ursodesoxycholic acid reduzate-containing medicament; the centrifugal part is used for carrying out precipitation separation on the dissolved medicament; the detector can accurately collect fluorescence signals of the immune complex; according to the ursodesoxycholic acid reduzate detection device, the integrated operation of detection test tube fixing, cover opening and transferring, medicament adding, cover closing and plug sealing and dissolving is achieved, the steps of automatic medicament filling, sealing and the like are achieved, the medicament preparation efficiency is greatly improved, efficient and accurate detection of ursodesoxycholic acid reduzate is further achieved, and the detection efficiency is improved. And complex and tedious manual operation is avoided, and the situation that the sample is easily polluted by manual operation is avoided.

The invention relates to the technical field of nano antibodies, in particular to an anti-FcRH5 nano antibody and application thereof. The invention provides an anti-FcRH5 nano antibody with high affinity and specificity, and the anti-FcRH5 nano antibody is based on a single-domain heavy chain variable region structure from camelidae and has the advantages of small molecular weight, high stability, strong tissue penetrability, easiness in engineering modification and the like. The nano antibody specifically recognizes and is combined with a high-expression and stable-expression target spot on the surface of a multiple myeloma cell through a complementary determining region of the nano antibody. The nano antibody disclosed by the invention can be used as a core recognition element for constructing various treatment or diagnosis tools such as chimeric antigen receptor T cells, bispecific antibodies, antibody drug conjugates or immunodetection probes and the like. According to the technical scheme, the technical problem that an anti-FcRH5 antibody with high quality and definite functionality is lacked in the prior art can be solved. The nano antibody provided by the invention has remarkable clinical transformation and industrialization advantages, and promotes multiple myeloma treatment to multi-target collaborative iteration.

The invention belongs to the field of immunodetection kits, and provides a P-Tau231 single-molecule immunodetection kit based on signal amplification and application of the P-Tau231 single-molecule immunodetection kit. The kit comprises a solid-phase carrier, a detection antibody-signal amplification probe conjugate and the like, wherein a total Tau non-phosphorylated region specific first antibody is fixed on the surface of the solid-phase carrier; the detection antibody-signal amplification probe conjugate is used for recognizing P-Tau231 phosphorylated 231 sites; quantitative detection is realized by adopting a sandwich immune sandwich mode and combining with a single-molecule detection platform. The detection limit of the kit reaches fg / mL to ag / mL, and the kit has the advantages of ultrahigh sensitivity, high specificity, simplicity and convenience in operation and low sample demand, can efficiently detect P-Tau231 in cerebrospinal fluid and plasma, and is suitable for preparation of early diagnosis products for Alzheimer's disease.

This invention belongs to the field of microcystin-LR detection technology, providing anti-microcystin-LR nanobodies and their applications. The anti-microcystin-LR nanobodies have amino acid sequences as shown in SEQ ID NO.1, SEQ ID NO.2, or SEQ ID NO.3. This invention uses phage display technology to screen for nanobodies with high affinity and high specificity for recognizing microcystin-LR, exhibiting advantages such as strong stability and ease of large-scale expression. Various detection tools can be designed based on anti-microcystin-LR, including test strips, immunoassay kits, immunoaffinity columns, biosensor detection chips, or detection probes. This provides novel antibody resources for rapid monitoring of microcystin-LR and lays a material foundation for developing low-cost, high-performance immunoassay technologies, which is of great significance for promoting the monitoring and control of microcystin-LR pollution.

The invention provides an alkaline phosphatase color developing solution and a preparation method thereof, and particularly relates to the technical field of immunodetection, the alkaline phosphatase color developing solution is formed by dissolving chromogen dry powder in a color developing buffer solution, and the concentration is 1-3 mg / ml; wherein the chromogen dry powder is composed of naphthol AS-MX phosphate and fast red-TR; the preparation method comprises the following steps: S1, dissolving naphthol AS-MX phosphate and fast red TR with purified water to obtain a solution; wherein the concentration of naphthol AS-MX phosphate in the solution is 0.5 to 1.5 mg / ml, and the concentration of fast red TR is 0.5 to 1.5 mg / ml; s2, freezing and storing the solution in a refrigerator, and condensing the solution into ice blocks; s3, the ice blocks are put into a freeze dryer, vacuumizing is carried out, water molecules in the ice blocks are sublimated into water vapor under the negative pressure condition, the water vapor is discharged, and chromogen dry powder can be obtained; s4, the chromogen dry powder is dissolved in the chromogenic buffer solution, and an alkaline phosphatase chromogenic solution can be obtained; the prepared developing solution has the core advantages of high sensitivity, rapid developing, multicolor compatibility, digital adaptation and the like, and an efficient and reliable tool is provided for pathological research and clinical diagnosis.

The invention comprises a micro-fluidic chip and an immunodetection method. The micro-fluidic chip comprises a reaction layer substrate and a micro-channel sample adding layer positioned on the reaction layer substrate; the reaction layer substrate comprises a base, a three-electrode part prepared on the base and a contact metal pad connected with an external circuit, and the three-electrode part comprises a working electrode, a reference electrode and a counter electrode; a plurality of micro-channel grooves are formed in the bottom of the micro-channel sample adding layer, a plurality of up-down through inflow holes and outflow holes are formed in the micro-channel sample adding channel layer, and the two ends of each micro-channel groove are communicated with the inflow holes and the outflow holes respectively. The micro-fluidic chip is designed through photoetching, PVD and other technologies, the chip comprises a plurality of micro-channels, interference of the external environment to the detection process can be reduced through testing in a closed environment, and the micro-fluidic chip has the rapid, stable and high-throughput detection function and can be applied to bedside detection.

A method for measuring the concentration of an analyte on the basis of a periodically variable external regulation force, and an oscillator system, which aim to solve the problem of non-specific adsorption in existing immunoassay techniques leading to reduced sensitivity and specificity in the measurement of analyte concentrations in samples. The measurement method comprises: obtaining a detection system, and setting a periodically variable external regulation force; binding both a capture probe and a detection probe with an analyte in a sample; making labeled particles, which are bound to the detection probe, perform periodic reciprocating motion under the action of the external regulation force; and by means of a monitoring and imaging apparatus, recording motion imaging signals of the labeled particles to select specifically bound molecules, and compiling statistics to calculate the proportion of the specifically bound molecules so as to obtain the analyte concentration. A specifically bound analyte can be accurately selected, thereby enhancing the capability and detection sensitivity of specific detection of analytes. The method has no complex elution processes, has a short detection response time, and exhibits high-sensitivity detection capability in complex environments.

The invention discloses an anti-phenacetin monoclonal antibody hybridoma cell strain AD, a preparation method and application thereof, and relates to the technical field of food safety immunodetection. The monoclonal antibody hybridoma cell strain is named monoclonal cell strain AD and the number CGMCC19681. The Phe-BA obtained by the hydrolysis of the reaction product of the phenacetin metabolite acetaminophen and ethyl 4-bromobutyrate is used as the hapten, and the hapten is coupled with the carrier protein to prepare the immunogen Phe-BA-BSA. After the mice were immunized with the immunogen Phe-BA-BSA, they were fused with myeloma cells by PEG method, screened by indirect competitive enzyme-linked immunosorbent assay and subcloned five times to obtain hybridoma cell lines. The monoclonal antibody secreted by the cell line can be made into a phenacetin detection kit, which has good affinity and detection sensitivity for phenacetin, and can be used for immunodetection of phenacetin residues in food.

This invention relates to a hybridoma cell line that secretes a Cy3.5 monoclonal antibody and its application, belonging to the field of immunoassay. The hybridoma cell line of this invention was deposited on November 12, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46741. The Cy3.5 monoclonal antibody secreted by this cell line exhibits excellent affinity and sensitivity to Cy3.5, with an IC50 of [missing value]. 50 Reaching 10 ng / mL, the IC50 for Cy5.5 50 An immunoassay product based on this hybridoma cell line, with a concentration of up to 50 ng / mL, can be used for the detection of Cy3.5 and its analogues, providing an efficient detection method and means for the residual detection of Cy3.5.