1496 results about "Immuno detection" patented technology

The invention discloses an intestinal cancer immune response graph neural network prediction method, a medium and equipment, and the method comprises the steps: collecting pathological image information, immunodetection information and basic clinical information, extracting a tissue space distribution characteristic spectrum through a deep convolutional network, and constructing a graph neural network model in combination with an immunomarker expression characteristic matrix; spatial interaction characteristics of a tumor microenvironment are modeled by adopting a graph attention mechanism, finally a treatment response probability, an optimal treatment opportunity and an adverse reaction risk are predicted through a multi-task learning framework, and a clinical decision report containing a prediction response curve, a risk early warning threshold and a treatment time window suggestion is output. According to the method, through multi-modal data fusion and spatial interaction modeling, accurate prediction of intestinal cancer immunotherapy response is realized, and a more comprehensive reference basis is provided for clinical decision making.

The invention discloses a bovine serum albumin combined nano antibody and a preparation method and application thereof, the amino acid sequence of the bovine serum albumin combined nano antibody Nb1 is shown as SEQ.ID No.1, the nano antibody Nb not only has high affinity to bovine serum albumin, but also has good binding activity to both human serum albumin and mouse serum albumin, and the nano antibody Nb has high affinity to human serum albumin and mouse serum albumin. And the antibody has a relatively good broad-spectrum application range, and can be widely applied to the aspects of drug half-life period enhancement, tumor targeted therapy and imaging, immunodetection or diagnosis, recombinant albumin purification or enrichment and the like.

The invention relates to a monoclonal antibody for detecting folic acid and a binding protein complex thereof or an antigen binding fragment thereof, and a preparation method and application thereof, the monoclonal antibody or the antigen binding fragment thereof comprises: (1) a heavy chain variable region having an amino acid sequence as shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3, HCDR1, HCDR2 and HCDR3, and (2) a heavy chain variable region having an amino acid sequence as shown in SEQ ID NO.3; the light chain variable region is provided with an LCDR1, an LCDR2 and an LCDR3, and the amino acid sequences of the LCDR1, the LCDR2 and the LCDR3 are respectively shown as SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6. The monoclonal antibody or the antigen binding fragment thereof can bind folic acid and a protein compound thereof with high specificity and high affinity, so that the detection accuracy and sensitivity of folic acid are improved, the limitation that a traditional competition method detection reagent is low in sensitivity and poor in clinical correlation is broken through, and sandwich method immunodetection of folic acid is realized.

The invention relates to the technical field of colloidal gold and fluorescence immunoassay detection, and discloses a fluorescence immunochromatography and colloidal gold double-platform detection method and device.The method comprises the steps that two-dimensional code information collection is carried out on a reagent card inserted into a cassette optical detection module, two-dimensional code information is obtained, and when a detection item is fluorescence immunoassay detection, the reagent card is detected to be the colloidal gold platform; collecting a fluorescence emission detection signal; when the detection item is colloidal gold detection, collecting a visible light reflection detection signal; carrying out smooth denoising processing and background correction to obtain a preprocessed detection signal; performing morphological analysis, and converting the two-dimensional image signal into one-dimensional waveform data to obtain effective signal intensity data; according to the method, the effective signal intensity data is subjected to absorbance integral quantitative calculation to obtain a T / C ratio, and the T / C ratio and the project calibration curve are converted to obtain the concentration value of the substance to be detected, so that integration of fluorescence immunodetection and colloidal gold detection is realized, and the accuracy and reliability of a detection result are improved.

The invention belongs to the technical field of immunodetection, and discloses a T3 sandwich method rabbit monoclonal antibody mAb3 and application thereof. The T3 sandwich method monoclonal antibody mAb3 provided by the invention comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises LCDR1-3, the LCDR1 comprises an amino acid sequence NIYNS, the LCDR2 comprises an amino acid sequence YAS, and the LCDR3 comprises an amino acid sequence QSHYGFSSSYGAA; the heavy chain variable region comprises HCDR1-3, the HCDR1 comprises an amino acid sequence GFDVSYN, the HCDR2 comprises an amino acid sequence IDTGANA, and the HCDR3 comprises an amino acid sequence ARVFYDNSFDP. The monoclonal antibody can be combined with a T3 compound in a high-specificity mode, is suitable for double-antibody sandwich immunodetection and is applied to an immunodetection kit prepared through a double-antibody sandwich ELISA or a chemiluminescence method, a T3 standard antigen is detected through a double-antibody sandwich chemiluminescence platform, the detection sensitivity is lower than 0.3 ng / ml, clinical samples are detected through a magnetochemiluminescence method, and the detection sensitivity is lower than 0.3 ng / ml. In the sample range of 1-80 pmol / L, the correlation with clinical comparison is good.

The present invention belongs to the field of biological detection technology, and specifically relates to an anti-monkey C-reactive protein monoclonal antibody, an enzyme-linked immunosorbent assay kit and its application. Named CRP-13, CRP-13 includes a heavy chain variable region and a light chain variable region; the heavy chain variable region has three complementary determining regions CDR-H1 to CDR-H3, and the amino acid sequences are shown in SEQ ID NO.6-SEQ ID NO.8; the light chain variable region has three complementary determining regions CDR-L1 to CDR-L3, and the amino acid sequences are shown in SEQ ID NO.9-SEQ ID NO.11. The monoclonal antibody of the present invention can specifically bind to monkey C-reactive protein with high specificity. The above monoclonal antibody can be used to screen a paired monoclonal antibody, and a monkey C-reactive protein enzyme-linked immunosorbent assay kit is obtained based on the combination of the monoclonal antibodies, which can detect the content of monkey C-reactive protein in monkey serum, plasma or tissue fluid.

The invention relates to a humanized nano antibody targeting cadherin 17 and application of the humanized nano antibody, and relates to the technical field of immunology and molecular biology. The complementarity determining region of the humanized nano antibody comprises a CDR1 of which the amino acid sequence is shown as SEQ ID NO: 2, a CDR2 of which the amino acid sequence is shown as SEQ ID NO: 4 and a CDR3 of which the amino acid sequence is shown as SEQ ID NO: 6; a framework region of the humanized nano antibody comprises an FR1 of which the amino acid sequence is shown as any one of SEQ ID NO: 1 and SEQ ID NO: 8, an FR2 of which the amino acid sequence is shown as any one of SEQ ID NO: 3, SEQ ID NO: 9 and SEQ ID NO: 11, an FR3 of which the amino acid sequence is shown as any one of SEQ ID NO: 5 and SEQ ID NO: 10, and an FR4 of which the amino acid sequence is shown as SEQ ID NO: 7. According to the humanized nano-antibody, the immunogenicity of the nano-antibody is reduced, the targeted binding activity and killing activity are improved, and the humanized nano-antibody can be used for developing immunodetection reagents, CAR-T / NK cell drugs and antibody drugs.

The present application discloses a dry electrochemiluminescent lateral flow immunoassay strip and its application in immunoassay, wherein the dry electrochemiluminescent lateral flow immunoassay strip comprises a lateral flow immunoassay strip, an electrode pad and a connection pad; the lateral flow immunoassay strip is composed of a sample pad, a binding pad, a detection pad with a backing NC membrane and an absorption pad stacked on a transparent adhesive plate; the electrode pad comprises an integrated closed bipolar electrode and a driving electrode; the integrated closed bipolar electrode comprises a closed bipolar electrode detection anode and a quality control anode and a shared cathode of the two anodes; the lateral flow immunoassay strip is inverted on the electrode pad; the T line and C line on the detection pad are in contact with the detection anode and the quality control anode on the electrode pad, respectively. The present invention adopts T / C for quantitative detection of biomarkers in ECL lateral flow immunoassay for the first time, solves the problem that the quality control signal is not fully utilized in the existing ECL lateral flow immunoassay, and improves the detection stability and storage stability.

The invention provides preparation of an anti-p-Tau217 antibody and application of the anti-p-Tau217 antibody in an Alzheimer's disease detection kit, and belongs to the technical field of antibodies and immunodetection, the anti-p-Tau217 antibody comprises a heavy chain variable region and a light chain variable region, a coding gene of the heavy chain variable region comprises a nucleotide sequence shown in SEQ ID NO.1, and a coding gene of the light chain variable region comprises a nucleotide sequence shown in SEQ ID NO.2. The coding gene of the light chain variable region comprises a nucleotide sequence shown in SEQIDNO.3, the heavy chain variable region comprises an amino acid sequence shown in SEQIDNO.2, and the light chain variable region comprises an amino acid sequence shown in SEQIDNO.3. The invention also discloses a preparation method. The invention provides preparation of an anti-p-Tau217 antibody and application of the anti-p-Tau217 antibody in an Alzheimer's disease detection kit. The successful expression of the monoclonal anti-p-Tau217 antibody with high affinity is realized, and the monoclonal anti-p-Tau217 antibody is used for detecting the Alzheimer's disease.

The invention provides a tumor detection data analysis method based on a peripheral blood immune marker, and belongs to the technical field of immunodetection.The tumor detection data analysis method comprises the steps that firstly, a peripheral blood sample of a patient is collected, and plasma and cell components are separated; analyzing various immune cell phenotypes by using flow cytometry; determining the concentration of key cell factors; analyzing the molecular expression level of the immune checkpoint; establishing an immune cell subset proportion empirical function; analyzing a cell factor expression mode by using an immune lineage transcriptome analysis model; establishing a tumor immune microenvironment score by combining the immune cell subset proportion and a model output result; the accuracy of the scoring system is verified through clinical follow-up visit and iconography examination; a standardized report template is formulated to provide a tumor early detection basis. Wherein the immune lineage transcriptome analytical model adopts a multi-modal attention fusion network architecture, three different immune data streams can be processed and integrated at the same time, and accurate evaluation of tumor-related immune states is realized.

The utility model relates to the field of microfluidic detection and fluorescence immunoassay, in particular to a portable biochemical blood coagulation and fluorescence immunoassay integrated analysis device, which comprises a shell, a power supply arranged in the shell, two support plates arranged in the shell, a fluorescent bracket fixedly connected to the upper ends of the support plates, a support arranged in the shell, a lamp source arranged at the upper end of the support, and a light source arranged at the lower end of the lamp source. A microfluidic detection module is mounted in the support, a fluorescence immunodetection module is mounted at the upper end of the fluorescence bracket, an optical module is arranged in the fluorescence immunodetection module, and a bar code scanner for confirming information of a detection chip or a reagent strip is arranged on one side of the optical module; compared with the traditional detection equipment with a single function, the double detection functions of biochemistry and fluorescence immunity are realized, the detection efficiency and comprehensiveness are greatly improved, meanwhile, the kit also supports the simultaneous detection of three reagent strips with different specifications, and the flexibility and convenience of the detection are further enhanced.

The invention discloses a detection method for B-type botulinum neurotoxin and application. The method disclosed by the invention is short in detection time, high in sensitivity as a qualitative detection method, capable of reaching a pictogram level, and obvious in detection advantage on trace toxin samples. Compared with an animal method using antiserum detection, the monoclonal antibody has high detection specificity, is beneficial to accurate decision-making when being used for scientific research detection and food hygiene inspection, and has small error compared with the animal method. In addition, the produced botulinum neurotoxin double-antibody sandwich reaction characteristic peak can be used for stability detection of botulinum neurotoxin, and plays a role which cannot be replaced by other methods in botulinum toxin product production, quality control, new product research and development, quality control and monitoring of standard substances for verification and market supervision. Therefore, the method can be used for guiding establishment of toxin immunodetection standard substances and promoting a capillary immunodetection technology to become a novel botulinum toxin detection standard method.

The invention belongs to the technical field of antibody preparation, and particularly relates to an anti-human carbohydrate antigen CA125 monoclonal antibody and application thereof. The amino acid sequences of CDR1-3 on a light chain variable region of the monoclonal antibody are respectively shown as SEQ ID NO.3-5, and the amino acid sequences of CDR1-3 on a heavy chain variable region of the monoclonal antibody are respectively shown as SEQ ID NO.8-10. The monoclonal antibody provided by the invention can specifically recognize and bind to a natural carbohydrate antigen CA125 expressed by human cells or tissues, has good affinity and relatively high sensitivity of binding of the antibody and the antigen, has strong anti-interference ability to complex non-target protein components in the cells / tissues, is beneficial to significantly improving the accuracy and reliability of immunodetection of human CA125 protein, and has good application prospects. The method is suitable for high-specificity, high-sensitivity and high-reliability detection of the human CA125 protein, and has good applicability in a plurality of detection systems such as immunoblotting, immunoprecipitation, immunohistochemistry and the like.

The invention provides a group of specific antibodies for detecting botulinum toxin A and application thereof, and belongs to the technical field of botulinum toxin detection. The invention provides a group of specific antibodies for detecting botulinum toxin A or antigen binding fragments thereof. The specific antibodies or the antigen binding fragments thereof comprise a rabbit monoclonal antibody 1F11 or an antigen binding fragment thereof and a rabbit monoclonal antibody 2A10 or an antigen binding fragment thereof. The time-resolved immunodetection kit prepared by the invention has good detection sensitivity, specificity, repeatability and sample matrix tolerance, the detection range is 0.02 ng / mL-10 ng / mL, the detection limit is 0.02 ng / mL, cross reaction with other serotype botulinum toxins (B, E and F) does not exist, and the time-resolved immunodetection kit is not influenced by sample matrixes such as ham sausages and thick broad-bean sauce. The detection system established by the invention has a good application prospect in detection of botulinum toxin type A.

The invention relates to an anti-PRV gD (pseudorabies virus gD) protein neutralizing monoclonal antibody, a hybridoma cell and application of the anti-PRV gD protein neutralizing monoclonal antibody. The anti-PRV gD neutralizing monoclonal antibody is prepared by constructing a PRV gD eukaryotic expression vector, transduction the PRV gD eukaryotic expression vector into a CHO (Chinese hamster ovary) cell for expression and then carrying out animal immunization by taking a PRV gD recombinant protein and an attenuated live vaccine as immunogens. The neutralizing monoclonal antibody or hybridoma cell provided by the invention lays a foundation for the development of a rapid detection technology of the pseudorabies virus and the gD protein thereof, has wide research and application values and commercial use values in immunodetection related to the pseudorabies virus, and has important guiding significance for vaccine development based on the pseudorabies virus.

The invention discloses a cat serum albumin combined nano antibody as well as a preparation method and application thereof, and relates to the technical field of nano antibody engineering. Wherein the amino acid sequence of the cat serum albumin binding nano antibody Nb1 is shown as SEQ.ID NO.1, and the nano antibody Nb1 not only has higher affinity to cat serum albumin, but also has better binding activity to mouse serum albumin; the polypeptide can be widely applied to the aspects of drug half-life period enhancement, tumor targeted therapy and imaging, immunodetection or diagnosis, recombinant albumin purification or enrichment and the like in the pet treatment process.

The invention belongs to the technical field of immunodetection, and discloses a rabbit monoclonal antibody mAb6 of an anti-mouse immune globulin G2a subtype (IgG2a) and application thereof. The rabbit monoclonal antibody mAb6 comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises LCDR1-3, the LCDR1 comprises an amino acid sequence KSVYKNNY, the LCDR2 comprises an amino acid sequence GTN, and the LCDR3 comprises an amino acid sequence AGGYID; the heavy chain variable region comprises HCDR1-3, the HCDR1 comprises an amino acid sequence RFSLSNYR, the HCDR2 comprises an amino acid sequence IFTRGST, and the HCDR3 comprises an amino acid sequence ARGWNS. The rabbit monoclonal antibody disclosed by the invention can be combined with a mouse immune globulin G2a subtype (IgG2a) in a high-specificity manner, can be applied to immunodetection, and provides a basis for further preparation of engineering antibodies.

The invention discloses a preparation method and application of composite nanoparticles with a gold-silver core-shell structure, and aims to solve the problems that silver halide precipitates are generated on the surface of a silver core and the silver core is etched in the prior art. The thiourea solution is used for replacing a halogen-containing reagent, uniform epitaxial growth of gold on the surface of the silver core is mildly achieved, the gold-shell silver-core nanoparticles which are evenly wrapped and stable in structure are prepared, and the gold-shell silver-core nanoparticles are suitable for the fields of immunodetection and the like and are good in color development effect and low in cost. The method comprises the steps of preparing a gold salt solution, preparing gold nano-seeds, preparing silver nano-cores and generating gold-shell silver-core nano-particles through a chemical reduction reaction, and the problem that a core-shell structure grows unevenly is solved. When the method is applied to immunodetection, the material cost can be remarkably reduced, and a better color development effect can be achieved.

The invention belongs to the technical field of immunodetection, and discloses an anti-T3 antibody mAb32 or an antigen binding fragment, the anti-T3 antibody mAb32 or the antigen binding fragment comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises LCDR1, LCDR2 and LCDR3, the amino acid sequence of the LCDR1 is shown as SEQ ID NO.1, the amino acid sequence of the LCDR2 is IAS, and the amino acid sequence of the LCDR3 is shown as SEQ ID NO.3; the heavy chain variable region comprises HCDR1, HCDR2 and HCDR3, and the amino acid sequences of the HCDR1, the HCDR2 and the HCDR3 are respectively shown as SEQ ID NO. 4-6. When the anti-T3 antibody mAb32 is used for detecting a T3 standard antigen, the detection sensitivity is lower than 0.2 ng / ml, and when a clinical sample is detected through a magnetochemiluminescence method, the correlation with R clinical comparison is good within the sample range of 1-80 pmol / L.

The invention provides an immunodetection method of a multi-index immune checkpoint detection kit, which belongs to the technical field of immunodetection and comprises three main stages of sample preparation, immune reaction and data analysis. The method comprises the following steps: firstly, carrying out centrifugal separation to obtain a plasma sample, and preparing a fluorescent microsphere coupled capture antibody and biotin labeled detection antibody compound; then carrying out immune reaction, forming a sandwich type immune complex through specific binding, and adding streptavidin marked by phycoerythrin for signal amplification. Detecting a fluorescence signal by adopting a flow cytometer to obtain an original data matrix; a contribution feature vector, a utility feature vector and an expression feature matrix are extracted through a feature decomposition algorithm, and a deep learning model of a total-score-total structure is established for data analysis. Finally, a detection report containing a quantitative result, an importance score and reliability evaluation is generated, and the technical problem that in the prior art, the analysis accuracy of a multi-index immune checkpoint detection result is insufficient is solved.

The invention relates to a human lactoferrin indirect competitive enzyme-linked immunosorbent assay kit and application thereof. The kit comprises an elisa plate, an anti-human lactoferrin antibody, an elisa secondary antibody, a confining liquid, a color developing agent and a stop solution. A nitrocellulose membrane in Western-Blot is replaced by an elisa plate with non-specific adsorption protein, and along with the addition of a sample, an anti-human lactoferrin antibody and an enzyme-labeled secondary antibody, a lactoferrin-primary antibody-secondary antibody compound is formed. And adding a color developing agent to carry out color development reaction, wherein the absorbance value of the sample is positively correlated with the content of the lactoferrin contained in the sample. And calculating the content of human lactoferrin in the sample according to the absorbance value. The method for detecting the human lactoferrin in the fermentation liquor has the advantages of reality, economy, high efficiency and the like.

The invention discloses an antibody or an antigen binding fragment thereof and a related product and application thereof, and relates to the field of immunodetection, the antibody comprises HCDR1, HCDR2 and HCDR3 in a heavy chain variable region of which the amino acid sequence is shown as SEQ ID NO: 28 and LCDR1, LCDR2 and LCDR3 in a light chain variable region of which the amino acid sequence is shown as SEQ ID NO: 29, the antibody can be specifically combined with HPV18 type E6-E7 fusion protein, and the antibody can be used for detecting the HPV18 type E6-E7 fusion protein. The method has the advantages of high detection sensitivity, good specificity, high accuracy, good repeatability and the like, and provides a way for effective detection of the HPV18 type E6-E7 fusion protein.

The invention relates to an anti-CK18 monoclonal antibody or an antigen binding fragment thereof, a detection reagent based on the anti-CK18 monoclonal antibody, and a preparation method and application of the anti-CK18 monoclonal antibody or the antigen binding fragment. According to the present invention, the anti-CK18 monoclonal antibody or the antigen binding fragment thereof can specifically bind with the CK18 protein or the fragment thereof with the high affinity, such that the low detection lower limit and the high detection sensitivity can be provided when the anti-CK18 monoclonal antibody or the antigen binding fragment thereof is used for the immunological detection of CK18; therefore, the monoclonal antibody or the antigen binding fragment of the monoclonal antibody has extremely high potential to be developed into an immunodetection antibody or a kit of the CK18 protein, or a diagnostic antibody or a diagnostic kit of diseases (such as NASH) related to abnormality of the CK18 protein (such as abnormal expression level), and has relatively great clinical application prospect and value.

The invention relates to the technical field of immunodetection and immunodiagnosis, in particular to a chemiluminescence substrate solution, a chemiluminescence detection kit and a detection method. The chemiluminescent substrate solution comprises a substrate solution 1, or comprises the substrate solution 1 and a substrate solution 2, the substrate solution 1 comprises sodium hydroxide, a surfactant and a blue light absorbent for absorbing blue light with the wavelength of 400-500 nm; the substrate solution 2 comprises inorganic acid and hydrogen peroxide. According to the application, the blue light absorbent is added into the chemiluminescent substrate liquid, so that blue light emitted by acridinium ester can be absorbed, a luminescent signal is reduced, and particularly, the luminescent value of a background caused by non-specific adsorption is reduced, so that the signal-to-noise ratio is increased.

The invention belongs to the technical field of immunodetection, and discloses a specific monoclonal antibody mAb48 of rabbit anti-human immunoglobulin G2 subtype (IgG2), the specific monoclonal antibody mAb48 comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises LCDR1-3, the LCDR1 comprises an amino acid sequence EDIGYG, the LCDR2 comprises an amino acid sequence GAS, and the LCDR3 comprises an amino acid sequence QQGFS; the heavy chain variable region comprises HCDR1-3, the HCDR1 comprises an amino acid sequence GFSLSNYE, the HCDR2 comprises an amino acid sequence ISSSGST, and the HCDR3 comprises an amino acid sequence ARNNNN. The monoclonal antibody disclosed by the invention can be combined with human immune globulin G2 subtype (IgG2) in a high-specificity manner, can be applied to immunodetection, and provides a basis for the preparation of engineering antibodies in the next step.

The invention belongs to the technical field of immunodetection, and discloses an anti-T3 antibody mAb68 or an antigen binding fragment, the anti-T3 antibody mAb68 or the antigen binding fragment comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises LCDR1, LCDR2 and LCDR3, the amino acid sequence of the LCDR1 is shown as SEQ ID NO.1, the amino acid sequence of the LCDR2 is TAS, and the amino acid sequence of the LCDR3 is shown as SEQ ID NO.3; the heavy variable region comprises HCDR1, HCDR2 and HCDR3, and the amino acid sequences of the HCDR1, the HCDR2 and the HCDR3 are respectively shown as SEQ ID NO. 4-6. When the anti-T3 antibody mAb68 is used for detecting a T3 standard antigen, the detection sensitivity is lower than 0.2 ng / ml, a clinical sample is detected through a magnetochemiluminescence method, and the correlation with R clinical comparison is good within the sample range of 1-80 pmol / L.

The invention discloses an immunodetection method based on holographic imaging mode conversion and deep learning, and belongs to the field of immunoassay detection. The deep learning holographic mode conversion system constructed based on the non-paired data set reduces the influence of diffraction interference on target quantification to the greatest extent, so that holographic super-resolution imaging is realized, and challenges in paired data set generation are overcome; besides, a functional glass slide is designed, and rapid and sensitive holographic imaging detection is realized in combination with a click reaction signal amplification strategy; the functionalized glass slide is combined with the portable holographic imaging equipment, so that the detection efficiency of the system is greatly improved while accurate signal amplification is carried out, the application scene is expanded, and antibiotic residues can be rapidly and sensitively detected. The invention opens up a new research direction for high-sensitivity on-site convenient detection of trace target objects in portable equipment and complex samples.

The invention discloses an enzyme-linked immunosorbent assay kit for detecting the content of soluble IL-6 (interleukin-6) protein. The enzyme-linked immunosorbent assay kit comprises two anti-human IL-6 monoclonal antibodies, namely, IL-6-1C7 and IL-6-2E11. The amino acid sequence of a heavy chain variable region of the IL-6-1C7 monoclonal antibody is SEQ ID NO.1, and the amino acid sequence of a light chain variable region of the IL-6-1C7 monoclonal antibody is SEQ ID NO.2; the amino acid sequence of a heavy chain variable region of the IL-6-2E11 is SEQ ID NO.3, and the amino acid sequence of a light chain variable region of the IL-6-2E11 is SEQ ID NO.4. The enzyme linked immunosorbent assay kit provided by the invention can effectively detect the content of soluble IL-6, and detection by using the kit finds that serum of healthy people contains soluble IL-6 protein with a certain basic level, the content of soluble IL-6 in serum of patients with inflammatory and autoimmune diseases and the like is obviously increased and is related to clinical staging and pathology of the patients, so that the content of soluble IL-6 in serum of patients with inflammatory and autoimmune diseases is obviously reduced. The detection of the content of the soluble IL-6 in the body fluid of the inflammatory patient has clinical value.

The invention relates to a hybridoma cell strain capable of secreting a napropamide-resistant monoclonal antibody and application of the hybridoma cell strain, and belongs to the field of food safety immunodetection. The hybridoma cell strain Tau4 secreting the napropamide cloned antibody is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is October 31, 2024, and the preservation number is CGMCC No.46242. The hybridoma cell strain Tau4 secreting the napropamide cloned antibody has the advantages that the hybridoma cell strain Tau4 secreting the napropamide The napropamide-resistant monoclonal antibody secreted by the cell strain is used for analyzing and detecting napropamide residues in food safety detection. The napropamide-resistant monoclonal antibody cell strain obtained by the invention can be used for immunoassay detection, and has better detection sensitivity and specificity (IC50 value is 1.13 ng / mL) on napropamide. The result of the invention can be used for preparing an immunodetection kit and a colloidal gold test strip of napropamide, and provides a powerful detection method and means for detecting the residual quantity of napropamide in foods such as fruits, vegetables and the like.

The invention relates to the technical field of immunochromatography, in particular to a silicon-coated color fluorescent microsphere and a mild preparation method and application thereof.The preparation method comprises the steps that firstly, time-resolved fluorescent dye is synthesized in an ethanol system, then oil-soluble dye, an ethanol water solution and a swelling agent dichloromethane are added, stirring is conducted at the room temperature to enable the dye to be dissolved, and then the silicon-coated color fluorescent microsphere is obtained; and adding ammonia water and a silane coupling agent tetraethyl orthosilicate, reacting overnight, supplementing the ammonia water, gradually adding a coupling agent 3-aminopropyltriethoxysilane, and washing with pure water for several times after the reaction is finished. The particle size range of the prepared color time-resolved fluorescent microspheres is 200-450 nm, the color time-resolved fluorescent microspheres are prepared in a green and mild mode through a one-step method, a surface active agent, a dispersing agent and a surface amino modified silicon dioxide matrix are not used, the high-luminous-intensity binuclear rare earth europium complex and the oil-soluble dye are embedded into the microspheres in the polymerization process, the dye is not prone to leakage, and the color time-resolved fluorescent microspheres are environmentally friendly and environmentally friendly. The method can be used for qualitative and quantitative dual immunodetection in the field of immunochromatography.