232 results about "Antibody labeling" patented technology

A method for determining a soluble human ST2 in a sample conveniently at a high sensitivity and an assay kit are provided. By an immunological method comprising a step for bringing a sample into contact with an immobilized antibody formed by binding to an insoluble support a first anti-human ST2 antibody which binds specifically to a non-denatured human ST2, a step for labelling a first reaction product generated in the previous step by reacting said first reaction product with a second anti-human ST2 antibody which binds specifically to a non-denatured human ST2 by recognizing a site different from the site on ST2 where said first anti-human ST2 antibody binds and which is labelled with a label, and a step for determining the amount of the label on said first reaction product which has been labelled, a soluble human ST2 in a sample is determined. In addition, a recombinant ST2 is employed as a standard to prepare a calibration curve, based on which the ST2 in a sample is quantified.

The invention provides a detection strip or kit for Zika virus detection by means of the fast immunochromatography method. The immunodetection strip has six areas. The first area is a sample adding pad; the second area is an immobilized Zika antigen marker area or anti-Zika virus antigen specific antibody marker; the third area is a detection area T and a coated antibody spray area, and the area T is matched with the second area; the fourth area is a contrast area used for immobilization of non-specific antibodies; the fifth area is a water absorber; the sixth area is a nitro fiber chromatography film. According to the detection strip or kit, only a small number of samples to be tested are needed, no equipment is required, a detection result can be obtained within ten minutes, detection is easy and fast, and the detection strip or kit can be purchased from a pharmacy so that patients can conduct detection by themselves.

The invention discloses an immunochromatographic test strip, a detection method by using the immunochromatographic test strip, and application of the immunochromatographic test strip; the immunochromatographic test strip comprises a conjugate cushion, a testing line and a quality control line; the conjugate cushion is coated with Janus nano-particles marked by a detection antibody; the testing line is coated with a capture antibody or antigen; and the quality control line is coated with an antibody of the detection antibody. On the basis of the Janus nano-particles, double functions including visual read-out and fluorescent read-out of a target object can be realized; compared with a traditional colloidal gold immunochromatographic test strip, for the immunochromatographic test strip, the quantitative detection can be realized; and the relatively high detection sensitivity can be obtained.

The magnetic separated AKP-labeled immunity analysis reagent comprises: the AKP label, the magnetic separation reagent as nano magnetic micro-ball covered by FITC, the FITC-antibody label, and the standard antigen or antibody and substrate. Wherein, the substrate can be PMP, AMPPD or APS-5. This invention is reliable and nun-polluted, and can detect fast and precisely.

Magnets and magnetic particle-labeled reagents are used to capture and/or release magnetic particle-tagged entities for immunohematology diagnostic testing purposes, especially tests performed in blood banking. The magnetic tagged entities may be tagged antibodies, tagged blood cells, tagged universal binding partners, especially tagged lectins and tagged Coombs reagent, and other binding agents such as biotin-avidin, Protein A or G, ligands and their receptors and the like. Separation of unbound material from bound material is effected through the use of one or both the magnetic field effect on the magnetic labeled reactants and the density gradients of layers of an assay construct. Constructs such as chromatographic strip lateral flow format, and liquid phase reactions in suitable vessels with end point determinations that do not require centrifugation to detect reacted entities. Readable labels such as enzymes, fluorophors, chemiluminescent materials, radioactive isotopes, and other labels may be attached to Coombs reagent to provide a readable product of the Coombs reagent with any antibody participating in the assay.

The invention discloses a microfluidic chip detection method based on magnetic bead technology. The method includes the steps of: 1, fluorescence labeling; 2, magnetic bead labeling; 3, coating and drying of a blocking solution; 4, immobilization of immunomagnetic beads; 5, immobilization of a fluorescence label; 6, microfluidic chip assembly; 7, sample adding preparation; 8, immunoreaction; 9, cleaning; 10, color development; and 11, data reading. The method provided by the invention selects highly sensitive time resolved fluorescent dye as the label for antibody labeling on magnetic beads and a fluorescent substance respectively, and utilizes the immunoreaction of the antibody pair for analysis and detection, and the performance of the prepared reagent can reach the level of equivalent chemiluminescent reagents. At the same time, the reagents adopt homogeneous liquid reaction to realize control of liquid flow. Ultrasonic mixing is adopted in the microfluidic chip chamber to avoid single-threaded chromatography flow reaction, therefore the reaction is fuller, and the reaction efficiency is higher.

The invention discloses an umbilical cord tissue mesenchymal stem cell isolated culture method. The method comprises the steps of A, sample collection and cell isolated culture; B, subculture, wherein after being cultured, primary cells are subcultured continuously when cell confluence reaches over 80%; C, growth curve drawing; D, cell detection, wherein the third generation of cells are adopted for detection, well digested third generation of umbilical cord mesenchymal stem cells are prepared and washed with a buffer solution, then antibody labeling is conducted on cells, incubation washing is conducted, and then detection is conducted. Two types of collagenase are used at the same time, and digestion of umbilical cord tissue is conducted with hyaluronidase and neutral protease or accutase, so that tissue blocks are quickly and fully digested.

The invention relates to a carbon quantum dot fluorescence labeling material with orange peels used as a carbon source as well as a preparation method and an application of the carbon quantum dot fluorescence labeling material. The orange peels are used as a raw material, and a simple method for one-step synthesis of a high-fluorescence-quantum-efficiency carbon quantum dot is established. The method comprises steps as follows: the orange peels are mixed with water; then a mixed liquid is mixed with concentrated sulfuric acid; the pH is adjusted by the aid of NaOH, and undissolved residues are removed in a centrifugal manner; then dichloromethane is added to a centrifuged supernate, and a non-fluorescence fat-soluble substance is removed in the centrifugal manner; finally, the centrifuged supernate passes through a film, and the fluorescence labeling material is prepared. According to the method, high-temperature and high-pressure reactions are not required to be performed by equipment such as plasma emitters, hydrothermal reaction kettles, muffle furnaces and the like during synthesis, reaction conditions are mild, operations are simple and convenient, the cost is very low, the method can be implemented in ordinary laboratories, the synthesized fluorescence labeling material lays a foundation for the further application to work such as bio-labeling, heavy metal analysis, antibody labeling, cell staining and the like.

The invention discloses a separation and purification method for fetal nucleated red blood cells and a Down syndrome screening kit and belongs to the detection field of molecular cell biology. The separation and purification method comprises the following steps: firstly, fully mixing maternal peripheral blood and a CD45 antibody labeling magnetic bead to adsorb white blood cells; discarding on a magnetic separator, transferring upper-layer cells to a test tube containing a CD71 antibody labeling magnetic bead, and fully and uniformly mixing; separating on the magnetic separator to obtain nucleated red blood cells; extracting a genome DNA (Deoxyribonucleic Acid) of the nucleated red blood cells; adding SRY (Sexdetermining Region Y), DSCR (Down Syndrome Critical Region) and USC2 specific probes, DSCR and USC2 competitive primers, an SRY specific primer and a DNA to be detected into the same competitive fluorescent quantitation PCR (Polymerase Chain Reaction) reaction system for multiple competitive real-time fluorescence quantification PCR reactions to obtain Cts of DSCR and USC2; and calculating a difference value between the Cts to judge whether a sample to be detected is highly risk to DS (Down Syndrome).

The invention relates to a preparation method and application of a malignant tumour specific growth factor (TSGF) antigen electrogenerated chemiluminescence sensor. The invention in particular relates to preparation and application of an electrogenerated chemiluminescence immunosensor taking aminated graphene GS-NH2 and an incubation substance of a detection antibody Ab2 as detection antibody labels and provides a simple, rapid and pollution-free preparation method and an application of a nitrogen doped carbon quantum dot electrogenerated chemiluminescence immunosensor. Linear range of the nitrogen doped carbon quantum dot electrogenerated chemiluminescence immunosensor is 0.01ng/mL-100ng/mL, and detection limit is 3.3pg/mL, so that rapid and sensitive detection on malignant TSGF antigen is realized.

An immune chromatographic test paper used for detecting antigen of legionella pneumophilia consists of sample pad, gold label pad of antibody label colloidal gold probe containing serum antigen resisting legionella pneumophilia, nitric acid fiber membrane and water absorption pad. It is featured as enveloping detection line containing antibody and quality control line containing anti-antibody on nitric acid fiber membrane and setting detection line to be separated from quality control line.

The invention discloses an immunization chromatography test paper for testing plasmodium and the manufacture method. The paper includes sample tray, gold mark tray, fiber cellulose membrane and sopping tray. The fiber cellulose membrane has a detection line and a quality control line. The detection line contains plasmodium specificity antibody. The invention has the advantages of convenience, sensitivity, specificity and rapidity.

The invention relates to a carboxylated fluorescent microsphere, a preparing method thereof and applications of the carboxylated fluorescent microsphere. The carboxylated fluorescent microsphere based on a conjugated polymer is prepared by steps of: preparing a poly(arylene ethynylene) polymer with a side chain containing carboxyl by utilization of a hydrolysis reaction method; activating the carboxyl with N-(3-dimethyllaminopropyl)-N'-ethyl carbodiimide hydrochloride and N-Hydroxysuccinimide; and bonding a fluorescent polymer onto an APGMA microsphere in a covalent bond manner by adopting a monodisperse amino-modified porous poly(glycidyl methacrylate) APGMA microsphere having a size of 5 [mu]m as a substrate sphere. The carboxylated fluorescent microsphere has biological reaction sites, and good biological coupling performance, and can detect the biomolecule BSA. If a needed antibody is coupled to the fluorescent microsphere through the carboxyl on the side chain of the polymer, a fluorescent microsphere with an antibody labeling is prepared and can be used for detection of the corresponding antigen.

The invention discloses an immune chromatographic indicator paper and making method of B-typed staphylococcus aureus enterotoxin, which comprises the following parts: sample pad 1, metal pad 2 with specific antibody mark colloidal gold probe of B-typed staphylococcus aureus enterotoxin in connection with one end of sample pad tightly, nitric fiber film (NC film) 3 in connection with the other end of metal pad tightly, water-absorbing pad 4 in connection with the other end of nitric fiber film tightly, wherein the nitric fiber film covers mutually separated detecting line 5 and quality control line 6, which is specific antibody of B-typed staphylococcus aureus enterotoxin and sheep-anti-rabbit lgG separately.

The invention discloses an immunochromatography test strip for detecting cystic echinococcosis and a preparation method thereof. The test strip comprises a hemofiltration membrane sample pad, a gold-marking pad, a cellulose membrane and a water absorption pad, wherein the gold-marking pad is tightly connected with the hemofiltration membrane sample pad and comprises an antibody labeling colloid gold probe of anti-echinococcus antigen advantage special antibody subtypes, the cellulose membrane is tightly connected with the gold-marking pad, the water absorption pad is tightly connected with the other end of the cellulose membrane, a detection line and a quality control line are arranged on the cellulose membrane, the detection line comprises crude antigens aiming at the cystic echinococcosis, and the quality control line comprises anti-antibodies capable of realizing special combination with the antibodies of the anti-echinococcus antigen advantage special antibody subtypes. The immunochromatography test strip of the invention has the advantages of simplicity, convenience, sensitivity, specificity and high speed, and is applicable to clinical and field use.

The invention discloses a human osteosarcoma stem-cell related antigenic marker stage-specific embryonic antigen-4 (SSEA-4) and an application thereof in a targeted therapy of osteosarcoma. The SSEA-4 is a specific osteosarcoma stem-cell surface marker which is almost not expressed in each normal adult tissue. The surface marker is easy for antibody labeling without affecting the cell viability, and applicable to the function tests after separation, therefore, a reliable molecular tracer is provided for further researching the function of stem cells in the origin, occurrence and development processes of osteosarcoma, and a foundation for explaining the clinical osteosarcoma drug resistance as well as transferring and looking for cellular and molecular targets for the targeted therapy is laid.

The invention provides a method and a reagent kit for detecting phosphatidylinositol proteoglycan 3 with a fluorescence immunochromatographic method. The method for detecting phosphatidylinositol proteoglycan 3 with the fluorescence immunochromatographic method comprises the steps that the fluorescence characteristic of rare earth fluorescent microspheres is utilized and combined with a mature immunochromatography technology, and fluorescent quantitative determination of phosphatidylinositol proteoglycan 3 is achieved by optimizing all steps. The reagent kit is characterized by being composed of a test paper card and an immunofluorescence chromatography analysis meter. A preparing method of the reagent kit comprises the steps of preparation of a specificity high-appetency monoclonal antibody, antibody labeling with rare earth fluorescent microspheres, preparation of a fluorescent microsphere combined pad and a nitrocellulose membrane sprayed with the antibody, assembling and wrapping of test paper strips, and the like. In the detection process, a sample is operated as a specification, a result is subjected to quantitative determination with the immunofluorescence chromatography analysis meter, and the advantages of being easy, convenient and fast to implement, suitable for field detection and the like are achieved.