235 results about "Antibody labeling" patented technology

Isolated, mammalian, adult bone marrow-derived, lineage negative hematopoietic stem cell populations (Lin− HSCs) contain endothelial progenitor cells (EPCs) capable of rescuing retinal blood vessels and neuronal networks in the eye. Preferably at least about 20% of the cells in the isolated Lin− HSCs express the cell surface antigen CD31. The isolated Lin− HSC populations are useful for treatment of ocular vascular diseases. In a preferred embodiment, the Lin− HSCs are isolated by extracting bone marrow from an adult mammal; separating a plurality of monocytes from the bone marrow; labeling the monocytes with biotin-conjugated lineage panel antibodies to one or more lineage surface antigens; removing of monocytes that are positive for the lineage surface antigens from the plurality of monocytes, and recovering a Lin− HSC population containing EPCs. Isolated Lin− HSCs that have been transfected with therapeutically useful genes are also provided, and are useful for delivering genes to the eye for cell-based gene therapy. Methods of preparing isolated stem cell populations of the invention, and methods of treating ocular diseases and injury are also described.

A method for determining a soluble human ST2 in a sample conveniently at a high sensitivity and an assay kit are provided. By an immunological method comprising a step for bringing a sample into contact with an immobilized antibody formed by binding to an insoluble support a first anti-human ST2 antibody which binds specifically to a non-denatured human ST2, a step for labelling a first reaction product generated in the previous step by reacting said first reaction product with a second anti-human ST2 antibody which binds specifically to a non-denatured human ST2 by recognizing a site different from the site on ST2 where said first anti-human ST2 antibody binds and which is labelled with a label, and a step for determining the amount of the label on said first reaction product which has been labelled, a soluble human ST2 in a sample is determined. In addition, a recombinant ST2 is employed as a standard to prepare a calibration curve, based on which the ST2 in a sample is quantified.

The present invention provides a universal, yet adaptable, anti-tag chimeric antigen receptor (AT-CAR) system which provides T cells with the ability and specificity to recognize and kill target cells, such as tumor cells, that have been marked by tagged antibodies. As an example, αFITC-CAR-expressing T cells have been developed that specifically recognize various human cancer cells when those cells are bound by cancer-reactive FITC-labeled antibodies. The activation of αFITC-CAR-expressing T cells is shown to induce efficient target lysis, T cell proliferation, and cytokine / chemokine production. The system can be used to treating subjects having cancer.

The invention provides a detection strip or kit for Zika virus detection by means of the fast immunochromatography method. The immunodetection strip has six areas. The first area is a sample adding pad; the second area is an immobilized Zika antigen marker area or anti-Zika virus antigen specific antibody marker; the third area is a detection area T and a coated antibody spray area, and the area T is matched with the second area; the fourth area is a contrast area used for immobilization of non-specific antibodies; the fifth area is a water absorber; the sixth area is a nitro fiber chromatography film. According to the detection strip or kit, only a small number of samples to be tested are needed, no equipment is required, a detection result can be obtained within ten minutes, detection is easy and fast, and the detection strip or kit can be purchased from a pharmacy so that patients can conduct detection by themselves.

The invention relates to a preparing method of a photoelectrochemical sensor based on sandwich cardiac troponin T marked by Ag2Se@CdSe and application, and belongs to the technical field of novel functional materials and biosensing detection. According to concrete contents of the preparing method and the application, CeO2-TiO2 composite nanometer materials with photoelectrocatalytic activity are used as optical activity substrate materials; Ag2Se@CdSe is used as an antibody marker; the sandwich photoelectrochemical sensor is prepared through the signal amplification effect of the marker Ag2Se@CdSe on the substrate materials, and is used for the high-sensitivity detection of the cardiac troponin T of myocardial damage specific marker. The method has the important significance on the early diagnosis and treatment of acute myocardial infarction.

The invention discloses an immunochromatographic test strip, a detection method by using the immunochromatographic test strip, and application of the immunochromatographic test strip; the immunochromatographic test strip comprises a conjugate cushion, a testing line and a quality control line; the conjugate cushion is coated with Janus nano-particles marked by a detection antibody; the testing line is coated with a capture antibody or antigen; and the quality control line is coated with an antibody of the detection antibody. On the basis of the Janus nano-particles, double functions including visual read-out and fluorescent read-out of a target object can be realized; compared with a traditional colloidal gold immunochromatographic test strip, for the immunochromatographic test strip, the quantitative detection can be realized; and the relatively high detection sensitivity can be obtained.

The invention discloses a nucleic acid detection method based on the polymer electrochemiluminescence signal amplification technology. The method comprises the following steps: synthesizing and activating ruthenium terpyridine, preparing a polylysine-ruthenium terpyridine compound, marking the polylysine-ruthenium terpyridine compound with a DNA (deoxyribonucleic acid) probe, and magnetically capturing and detecting a target DNA sequence. According to the method, the polymer electrochemiluminescence signal amplification technology is applied to nucleic acid detection, and an organic polymer such as polylysine is good in physical and chemical properties and can be applied to the field of electrochemiluminescence signal amplification; polylysine is adopted as a connecting framework for ruthenium terpyridine signal amplification, and the system is stable, hard to aggregate and easy to store; the polylysine-ruthenium terpyridine compound marked with the DNA probe is stable in properties and convenient to modify and store. The method is easy in operation, quick, stable and controllable, is suitable for nucleic acid detection and can serve as an antibody labeling method in immunoassay to improve the sensitivity.

The invention provides a CRP latex-reinforced immunonephelometry reagent. The CRP latex-reinforced immunonephelometry reagent comprises CRP antibody-labeled latex particles, a buffer, a surfactant, an inorganic salt, a stabilizing agent, a suspending assistant, an excipient and an antiseptic. The invention also provides a kit for single-reagent CRP latex-reinforced immunonephelometry and a use thereof. The CRP latex-reinforced immunonephelometry reagent has simple composition, can be used simply and conveniently, has a fast reaction rate, high sensitivity and good repeatability, can be operated by a simple apparatus, has a wide apparatus adaptation range, has a low cost, can be widely used in large, middle and small hospitals and is suitable for clinical fast infection diagnosis and infection prognosis evaluation.

The magnetic separated AKP-labeled immunity analysis reagent comprises: the AKP label, the magnetic separation reagent as nano magnetic micro-ball covered by FITC, the FITC-antibody label, and the standard antigen or antibody and substrate. Wherein, the substrate can be PMP, AMPPD or APS-5. This invention is reliable and nun-polluted, and can detect fast and precisely.

Magnets and magnetic particle-labeled reagents are used to capture and / or release magnetic particle-tagged entities for immunohematology diagnostic testing purposes, especially tests performed in blood banking. The magnetic tagged entities may be tagged antibodies, tagged blood cells, tagged universal binding partners, especially tagged lectins and tagged Coombs reagent, and other binding agents such as biotin-avidin, Protein A or G, ligands and their receptors and the like. Separation of unbound material from bound material is effected through the use of one or both the magnetic field effect on the magnetic labeled reactants and the density gradients of layers of an assay construct. Constructs such as chromatographic strip lateral flow format, and liquid phase reactions in suitable vessels with end point determinations that do not require centrifugation to detect reacted entities. Readable labels such as enzymes, fluorophors, chemiluminescent materials, radioactive isotopes, and other labels may be attached to Coombs reagent to provide a readable product of the Coombs reagent with any antibody participating in the assay.

An optical biological detector is able to bind specific targeted bacterial cells by stamping an antibody grating pattern onto a silicon surface. The antibody grating alone produces insignificant optical diffraction, but upon immunocapture of the targeted cells, the optical phase change produces a diffraction pattern. Micro-contact printing provides a method for placing the antibody grating pattern directly onto a substrate surface with no additional processes or binding chemicals. Antibodies or other biologically active material may be stamped directly onto clean native oxide silicon substrates with no other chemical surface treatments. Direct binding of the antibodies to the silicon occurs in a way that still allows them to function and selectively bind antigen. The performance of the sensor was evaluated by capturing Escherichia coli O157:H7 cells on the antibody-stamped lines and measuring the intensity of the first order diffraction beam resulting from the attachment of cells. The diffraction intensity increases in proportion to the cell density bound on the surface.

The invention discloses a microfluidic chip detection method based on magnetic bead technology. The method includes the steps of: 1, fluorescence labeling; 2, magnetic bead labeling; 3, coating and drying of a blocking solution; 4, immobilization of immunomagnetic beads; 5, immobilization of a fluorescence label; 6, microfluidic chip assembly; 7, sample adding preparation; 8, immunoreaction; 9, cleaning; 10, color development; and 11, data reading. The method provided by the invention selects highly sensitive time resolved fluorescent dye as the label for antibody labeling on magnetic beads and a fluorescent substance respectively, and utilizes the immunoreaction of the antibody pair for analysis and detection, and the performance of the prepared reagent can reach the level of equivalent chemiluminescent reagents. At the same time, the reagents adopt homogeneous liquid reaction to realize control of liquid flow. Ultrasonic mixing is adopted in the microfluidic chip chamber to avoid single-threaded chromatography flow reaction, therefore the reaction is fuller, and the reaction efficiency is higher.

The invention discloses an umbilical cord tissue mesenchymal stem cell isolated culture method. The method comprises the steps of A, sample collection and cell isolated culture; B, subculture, wherein after being cultured, primary cells are subcultured continuously when cell confluence reaches over 80%; C, growth curve drawing; D, cell detection, wherein the third generation of cells are adopted for detection, well digested third generation of umbilical cord mesenchymal stem cells are prepared and washed with a buffer solution, then antibody labeling is conducted on cells, incubation washing is conducted, and then detection is conducted. Two types of collagenase are used at the same time, and digestion of umbilical cord tissue is conducted with hyaluronidase and neutral protease or accutase, so that tissue blocks are quickly and fully digested.

The invention relates to a carbon quantum dot fluorescence labeling material with orange peels used as a carbon source as well as a preparation method and an application of the carbon quantum dot fluorescence labeling material. The orange peels are used as a raw material, and a simple method for one-step synthesis of a high-fluorescence-quantum-efficiency carbon quantum dot is established. The method comprises steps as follows: the orange peels are mixed with water; then a mixed liquid is mixed with concentrated sulfuric acid; the pH is adjusted by the aid of NaOH, and undissolved residues are removed in a centrifugal manner; then dichloromethane is added to a centrifuged supernate, and a non-fluorescence fat-soluble substance is removed in the centrifugal manner; finally, the centrifuged supernate passes through a film, and the fluorescence labeling material is prepared. According to the method, high-temperature and high-pressure reactions are not required to be performed by equipment such as plasma emitters, hydrothermal reaction kettles, muffle furnaces and the like during synthesis, reaction conditions are mild, operations are simple and convenient, the cost is very low, the method can be implemented in ordinary laboratories, the synthesized fluorescence labeling material lays a foundation for the further application to work such as bio-labeling, heavy metal analysis, antibody labeling, cell staining and the like.

The invention relates to a preparation method and application of a pancreatic cancer immunosensor based on gold electrodeposition and Au@Ag / CuO-GS as markers, and particularly relates to preparation of a sandwich-type electrochemical immunosensor for detecting pancreatic cancer tumor markers by adopting Au@Ag / CuO-GS as a detection antibody marker. By using excellent biocompatibility and high catalytic performance of the Au@Ag / CuO-GS, the prepared sensor has relatively high sensitivity and relatively wide detection range; and the detection limit can reach 1.5fg / mL.

The invention relates to a quantum dot fluorescent probe and application thereof. The invention is characterized in that the quantum dot fluorescent probe utilizes a difunctional cross-linking agent long-chain succinimidyl-4-[N-maieimidomethyl]cyclohexane-1-carboxy-[6-amidocaproate] (SMCC) to undergo a cross-linking reaction with an amino group on the surface of an amino quantum dot; a maleimide group is produced on the surface of the quantum dot; a to-be-labeled antibody is reduced by a reducing agent dithiothreitol (DTT) so as to reduce a disulfide bond into a mercapto group; and the mercapto group and the maleimide group form a covalent bond so as to realize labeling of the quantum dot with the antibody. The quantum dot fluorescent probe is applied to (1) detection of one protein oncofetal antigen or (2) detection of a plurality of protein CEA, euron-specific enolase (NSA) and a cytokerain fragment 19CYFRA21-1, wherein the detection limit of detection (1) is 38 pg / ml, and the detection limit of detection (2) is 0.9 ng / ml.

The invention discloses a separation and purification method for fetal nucleated red blood cells and a Down syndrome screening kit and belongs to the detection field of molecular cell biology. The separation and purification method comprises the following steps: firstly, fully mixing maternal peripheral blood and a CD45 antibody labeling magnetic bead to adsorb white blood cells; discarding on a magnetic separator, transferring upper-layer cells to a test tube containing a CD71 antibody labeling magnetic bead, and fully and uniformly mixing; separating on the magnetic separator to obtain nucleated red blood cells; extracting a genome DNA (Deoxyribonucleic Acid) of the nucleated red blood cells; adding SRY (Sexdetermining Region Y), DSCR (Down Syndrome Critical Region) and USC2 specific probes, DSCR and USC2 competitive primers, an SRY specific primer and a DNA to be detected into the same competitive fluorescent quantitation PCR (Polymerase Chain Reaction) reaction system for multiple competitive real-time fluorescence quantification PCR reactions to obtain Cts of DSCR and USC2; and calculating a difference value between the Cts to judge whether a sample to be detected is highly risk to DS (Down Syndrome).

The invention belongs to the technical field of nano functional materials, immunoassay and biosensing, and provides a preparation method and application of an immunosensor established based on a manganese dioxide loaded silver nanoparticle multiwalled carbon nanotube. The electrochemical immunosensor which is prepared by taking the manganese dioxide loaded silver nanoparticle multiwalled carbon nanotube AgNPS@MnO2@MWCNTs as a detection antibody marker has the advantages of high specificity, high sensitivity, low detection limit and the like, and has significant scientific meanings and application values in detection on carcino-embryonic antigen CEA and alpha fetoprotein AFP.

The invention relates to a preparation method and application of a malignant tumour specific growth factor (TSGF) antigen electrogenerated chemiluminescence sensor. The invention in particular relates to preparation and application of an electrogenerated chemiluminescence immunosensor taking aminated graphene GS-NH2 and an incubation substance of a detection antibody Ab2 as detection antibody labels and provides a simple, rapid and pollution-free preparation method and an application of a nitrogen doped carbon quantum dot electrogenerated chemiluminescence immunosensor. Linear range of the nitrogen doped carbon quantum dot electrogenerated chemiluminescence immunosensor is 0.01ng / mL-100ng / mL, and detection limit is 3.3pg / mL, so that rapid and sensitive detection on malignant TSGF antigen is realized.

The invention provides an immunochromatographic assay system. The system comprises a housing and a laser light source, a test strip mounting base, a chromatographic test strip and a camera arranged inthe housing; the chromatographic test strip is placed on the test strip mounting base, the light produced by the laser light source is used to irradiate the chromatographic test strip, and the camerais used to capture a fluorescent signal generated after the chromatographic test strip is irradiated. According to the immunochromatographic assay system of the invention, the laser light source is used to irradiate quantum dot microspheres that are labeled by detection antibodies of various biomarkers and have different colors, a plurality of biomarkers can be simultaneously detected at one timeand imaged for data analysis, and the detection process is accurate and efficient. Based on the operation, the invention also provides a sample imaging method and a sample image analysis method basedon the above immunochromatographic assay system.

An immune chromatographic test paper used for detecting antigen of legionella pneumophilia consists of sample pad, gold label pad of antibody label colloidal gold probe containing serum antigen resisting legionella pneumophilia, nitric acid fiber membrane and water absorption pad. It is featured as enveloping detection line containing antibody and quality control line containing anti-antibody on nitric acid fiber membrane and setting detection line to be separated from quality control line.

The invention discloses a method of detecting ochratoxin A in cereals by means of a nano antibody and immunomagnetic separation. The method includes the steps of: 1) combining anti-OTA nano antibody with nano immunomagnetic beads to prepare IMB, and enriching the OTA in the cereals by means of the IMB to prepare a pretreated sample; 2) with dot immunization method, immobilizing an OTA-OVA detection antigen on a PVDF film, wherein the immunomagnetic beads are subjected to an immune-reaction in the form of antibody marker, the OTA-OVA and the to-be-tested substance, OTA, are combined with the antibody competitively so as to determine whether the immunomagnetic beads are aggregated on the PVDF film or not, thereby obtaining a detection result. The method achieves simple and quick qualitative detection. An operator can read the result just by naked eyes without any analysis instrument. Furthermore, the method can be used for developing a novel quick and visible detection card for specially detecting the OTA in the cereals.

The invention discloses an immunization chromatography test paper for testing plasmodium and the manufacture method. The paper includes sample tray, gold mark tray, fiber cellulose membrane and sopping tray. The fiber cellulose membrane has a detection line and a quality control line. The detection line contains plasmodium specificity antibody. The invention has the advantages of convenience, sensitivity, specificity and rapidity.

The invention relates to a carboxylated fluorescent microsphere, a preparing method thereof and applications of the carboxylated fluorescent microsphere. The carboxylated fluorescent microsphere based on a conjugated polymer is prepared by steps of: preparing a poly(arylene ethynylene) polymer with a side chain containing carboxyl by utilization of a hydrolysis reaction method; activating the carboxyl with N-(3-dimethyllaminopropyl)-N'-ethyl carbodiimide hydrochloride and N-Hydroxysuccinimide; and bonding a fluorescent polymer onto an APGMA microsphere in a covalent bond manner by adopting a monodisperse amino-modified porous poly(glycidyl methacrylate) APGMA microsphere having a size of 5 [mu]m as a substrate sphere. The carboxylated fluorescent microsphere has biological reaction sites, and good biological coupling performance, and can detect the biomolecule BSA. If a needed antibody is coupled to the fluorescent microsphere through the carboxyl on the side chain of the polymer, a fluorescent microsphere with an antibody labeling is prepared and can be used for detection of the corresponding antigen.

The invention discloses preparation and application of an adjacent hybridization dual-mode immunosensor based on MXene nanosheet photo-thermal amplification. Polyethyleneimine (PEI) modified TiO2 disc-shaped nanoparticles are used as a substrate, and a large amount of capture substrate deoxyribonucleic acids are fixed on a sensing interface through electrodeposition of gold. Two deoxyribonucleic acid probes are both labeled with a human epididymis protein 4 (HE4) antibody of a target object, and after the deoxyribonucleic acid probes as a recognition part of HE4 are captured by DNA3, the HE4 induces adjacent hybridization between primary antibody-labeled DNA1 and secondary antibody-labeled DNA2 by immunorecognition. An MXene nanosheet is loaded with a large amount of thionine, so that theMXene nanosheet can be effectively intercalated into a double-stranded DNA groove formed by hybridization, can be used as a signal probe for electrochemical-temperature dual modes, and realizes amplification of electrochemical signals due to temperature rise generated by the photo-thermal effect. Under the irradiation of an 808 nm infrared laser device, high-sensitivity detection of the HE4 is realized.

The invention discloses an immune chromatographic indicator paper and making method of B-typed staphylococcus aureus enterotoxin, which comprises the following parts: sample pad 1, metal pad 2 with specific antibody mark colloidal gold probe of B-typed staphylococcus aureus enterotoxin in connection with one end of sample pad tightly, nitric fiber film (NC film) 3 in connection with the other end of metal pad tightly, water-absorbing pad 4 in connection with the other end of nitric fiber film tightly, wherein the nitric fiber film covers mutually separated detecting line 5 and quality control line 6, which is specific antibody of B-typed staphylococcus aureus enterotoxin and sheep-anti-rabbit lgG separately.

The invention discloses an immunochromatography test strip for detecting cystic echinococcosis and a preparation method thereof. The test strip comprises a hemofiltration membrane sample pad, a gold-marking pad, a cellulose membrane and a water absorption pad, wherein the gold-marking pad is tightly connected with the hemofiltration membrane sample pad and comprises an antibody labeling colloid gold probe of anti-echinococcus antigen advantage special antibody subtypes, the cellulose membrane is tightly connected with the gold-marking pad, the water absorption pad is tightly connected with the other end of the cellulose membrane, a detection line and a quality control line are arranged on the cellulose membrane, the detection line comprises crude antigens aiming at the cystic echinococcosis, and the quality control line comprises anti-antibodies capable of realizing special combination with the antibodies of the anti-echinococcus antigen advantage special antibody subtypes. The immunochromatography test strip of the invention has the advantages of simplicity, convenience, sensitivity, specificity and high speed, and is applicable to clinical and field use.

The invention discloses a human osteosarcoma stem-cell related antigenic marker stage-specific embryonic antigen-4 (SSEA-4) and an application thereof in a targeted therapy of osteosarcoma. The SSEA-4 is a specific osteosarcoma stem-cell surface marker which is almost not expressed in each normal adult tissue. The surface marker is easy for antibody labeling without affecting the cell viability, and applicable to the function tests after separation, therefore, a reliable molecular tracer is provided for further researching the function of stem cells in the origin, occurrence and development processes of osteosarcoma, and a foundation for explaining the clinical osteosarcoma drug resistance as well as transferring and looking for cellular and molecular targets for the targeted therapy is laid.

The invention provides a fluorescent protein and / or coupled protein monoclonal antibody labeling method and a kit thereof. The preparation method comprises the following steps: firstly, carrying out desalination treatment on fluorescent protein and / or coupled protein, blocking sulfydryl of the fluorescent protein and / or coupled protein by adopting NEM, and carrying out cross-linking reaction on the fluorescent protein and / or coupled protein NEM and S-SMCC; meanwhile, cross-linking SLCSPDP and the monoclonal antibody, reducing disulfide bonds of the monoclonal antibody through TCEP, and conducting sulfhydrylation on the monoclonal antibody; and finally, carrying out cross-linking reaction on the sulfhydrylated antibody and NEM-fluorescent protein and / or coupled protein-S-SMCC. Directional coupling of the fluorescent protein and / or the coupling protein and the monoclonal antibody is achieved, the cross-linking efficiency of labeling of the fluorescent protein and / or the coupling proteinand the monoclonal antibody is improved, the specificity of labeling of the fluorescent protein and / or the coupling protein and the monoclonal antibody is further improved, and a fluorescence signal is enhanced.

The invention relates to immunochromatography technologies, and aims at providing a bigeminy qualitative fungaltoxin colloidal gold immunochromatography test strip and a preparation method. The test strip comprises a black strip-like polyvinyl chloride backboard taken as a base material, wherein the surface of the test strip is successively provided with a sample pad, a colloidal gold antibody marker fixing pad, a nitrocellulose membrane and an adsorption pad along the length direction of the backboard, and the adjacent pad or the membrane are in overlap joint with each other; and the nitrocellulose membrane is provided with two detection lines and one quality control line which have the same width with that of the backboard and are separated from one another, wherein the quality control line is positioned between the two detection lines. According to the test strip provided by the invention, the colloidal gold, the cost of which is relatively low, is adopted as an antibody marker, a competition method principle is adopted to quickly detect fungaltoxin, the nitrocellulose membrane wraps the two detection lines simultaneously, the color depth can be compared by naked eyes, and qualitative detection can be carried out quickly on two kinds of fungaltoxin simultaneously. The chromatography test strip prepared has excellent sensitivity and stability, and is easy, convenient and fast to operate and low in cost, thereby being particularly suitable for rapid diagnosis on site.