67 results about "Antibody labeling" patented technology

The invention provides a micro-fluidic chip for combined detection of C-reactive protein and ferritin and application thereof.The micro-fluidic chip comprises a substrate and a cover plate pressed on the substrate, a micro-channel is defined by the substrate and the cover plate, the left end of the micro-channel is communicated with a buffer solution sample adding hole formed in the cover plate, the right end of the micro-channel is communicated with a waste liquid area, and the left end of the micro-channel is communicated with a buffer solution sample adding hole formed in the cover plate. The waste liquid area is provided with a movable water-absorbing paper sheet, the microchannel is sequentially provided with a marking area, a detection area and a sample adding area from left to right, the marking area is provided with a marked anti-human CRP antibody, a marked anti-human SF antibody, a marked second antibody and a marked antigen, the first detection site is provided with an anti-human CRP antibody, and the second detection site is provided with an anti-human SF antibody. The first reference point is provided with a first antibody which reacts with the labeled second antibody, the second detection site is provided with an anti-human SF antibody, and the second reference point is provided with an antibody which reacts with the labeled antigen. The detection process of C-reactive protein and ferritin can be simplified, and the detection time is shortened.

This invention belongs to the field of microfluidics, and particularly relates to the use of a pine pollen microrobot combined with a microfluidic chip for cell capture. The pine pollen microrobot is gold-sputtered pine pollen, and the microfluidic chip is injected with the pine pollen microrobot and a conductive cell solution. When an alternating current signal is applied to the microfluidic chip, the fluid surrounding the pine pollen microrobot generates an induced charge electroosmotic vortex, causing cells in the cell solution to move toward the cavity of the pine pollen microrobot, thereby achieving cell capture. Using induced charge electroosmosis (ICEO) technology, this invention enables the efficient capture of blood cells and other micron-sized cell structures. This solves the problem of traditional capture techniques relying on antibody labeling and mechanical capture, reduces operational complexity and capture difficulty, and improves capture success rate and raw material utilization.

The invention discloses a culture method for inducing high expression of umbilical cord mesenchymal stem cells (LRP1) and application, and belongs to the technical field of biological medicines. The technical problem to be solved is to provide a standardized culture method for directionally inducing a specific functional phenotype cell population, so that efficient and accurate induction of LRP1 high-expression UCMSCs is realized, and clinical adaptation is met. According to the key point of the technical scheme, the culture method for inducing the LRP1 high-expression umbilical cord mesenchymal stem cells comprises the following steps: inoculating umbilical cord mesenchymal stem cells into a culture dish at the inoculation density of 5.5 * 10 < 3 > / cm < 2 > to 3.6 * 10 < 4 > / cm < 2 >, and culturing for 24-72 hours by using a special mesenchymal stem cell culture medium without adding a supplement, so that the acquisition efficiency of the LRP1 high-expression UCMSCs is improved, and the yield of the LRP1 high-expression UCMSCs is increased. Antibody labeling and flow sorting are not needed, so that the operation steps are simplified, and the complexity and cost of the culture process are reduced.

This invention discloses a quantitative test strip for HBV PreS1 antibody based on the AIE molecule, its preparation method, and its applications, relating to the fields of molecular biology, immunology, and biodetection technology. The test strip includes a sample pad, a labeling pad, a nitrocellulose membrane (NC membrane), an absorbent pad, and a base plate. The NC membrane detection line is coated with HBV PreS1 antigen to capture PreS1 antibodies in the sample. The labeling pad contains an immobilized Anti-Human IgG antibody conjugated to the AIE molecule, which binds to the PreS1 antibodies in the sample to form an immune complex. The test strip of this invention enables rapid detection of PreS1 antibodies and achieves enhanced fluorescence in the detection results. Combined with a fluorescence reader or fluorescence card reader, the detection results can be digitally and quantitatively analyzed. It is simple to operate, rapid in detection, and significantly improves detection sensitivity and enables quantitative analysis.

The invention relates to a method for detecting activation of specific T cells after tuberculosis infection and application of the method. Specifically, the invention provides a reagent combination for detection, and the reagent combination comprises an anti-CD4 antibody (CD4 antibody), an anti-CD154 antibody (CD154 antibody), an IFN-gamma detection antibody and a tuberculosis specific antigen. According to the method, through peripheral whole blood collection, PBMC cell separation, T cell activation (activation), activation termination (blocking), antibody labeling, membrane rupture, fixation, intracellular cytokine dyeing, flow cytometry detection and result analysis and judgment, the ratio of the expression IFN-gamma cells in the CD4 + CD154 + cells can be rapidly and accurately determined, and by combining an ROC curve and a constructed judgment standard, the content of the IFN-gamma cells in the CD4 + CD154 + cells can be determined, and the content of the IFN-gamma cells in the CD4 + CD154 + cells in the CD4 + CD154 + cells in the CD4 + CD154 + cells in the CD4 + CD154 + cells can be determined. And the sample to be detected is judged to be in a positive / negative / gray region, so that whether a patient is infected by the mycobacterium tuberculosis or not can be more accurately diagnosed, especially aiming at a whole blood sample from a special crowd.

The application discloses an acridinium ester labeled complex, which comprises a body, and a labeling group labeled on the body, wherein the body is an antibody, and the labeling group is an acridinium ester group. A preparation method of the acridinium ester labeled complex comprises the following steps: sucking a labeling buffer into a centrifugal tube; sucking an antibody to be labeled into the centrifugal tube and mixing to generate a first mixed solution; adding an acridinium ester solution and mixing, and then performing reaction at 25 DEG C in the dark for 2 hours to generate a second reaction solution; adding a termination buffer and mixing, and then performing reaction at 25 DEG C in the dark for 30 minutes to generate a third reaction solution; performing desalting purification on the third reaction solution to generate a fourth mixed solution; and filtering the fourth mixed solution with a 0.22 mu m filter membrane to generate the acridinium ester labeled complex. According to the preparation method, the labeling efficiency of the acridinium ester and the antibody is higher, the reactivity is stronger, the non-specific adsorption is lower, the sensitivity is higher, the repeatability and stability are better, and the labeling method is simple, rapid and easy to repeat.

This disclosure provides a method for analyzing peripheral blood leukocytes. In some embodiments, method may comprise: (a) labeling peripheral blood leukocytes isolated from a patient that has or is suspected of having inflammatory bowel disease (IBD) with a panel of distinguishably-labeled antibodies and (b) analyzing binding of the antibodies to the peripheral blood leukocytes. Kits for performing the method are also provided. Results from the analysis can be used to make a diagnosis of Crohn's disease or ulcerative colitis.

The invention relates to a method for amplifying an acridine system of a chemiluminescence platform, and particularly provides a method for enhancing the luminous intensity in chemiluminescence immunoassay, which comprises the step of enhancing the luminous intensity by using a composition, and the composition comprises biotin labeled by an anti-acridine analogue antibody and streptavidin labeled by an acridine analogue. According to the method, the sensitivity and the luminescence value are amplified by utilizing the specific binding of the biotin and the avidin and then utilizing the superposition of the specific affinity binding mode of the acridine analogue and the acridine analogue antibody, the sensitivity can be improved by 5-10 times on the existing acridine analogue platform, and the application prospect is excellent.

The invention discloses a universal test strip for rapid detection of multiple nucleic acid amplification products as well as a preparation method and application of the universal test strip, and belongs to the technical field of rapid detection of nucleic acid. The universal test strip comprises a reaction bottom plate, a sample pad, a combination pad, a chromatography membrane and a water absorption pad, wherein the combination pad is loaded with a marked first target antibody, a marked first quality control antibody or antigen; the chromatography membrane is provided with a detection line T coated with a second target antibody and a quality control line C coated with a second quality control antibody or antigen; the detection line and the quality control line are sequentially arranged on the chromatography membrane in parallel at intervals along the chromatography direction. The universal test strip has the remarkable advantages of simplicity and convenience in operation, high detection speed, high sensitivity, strong specificity, low cost and the like, is wide in application technical range, is suitable for various nucleic acid detection technical methods, has strong tolerance to sample inhibitors, and is particularly suitable for being combined with isothermal amplification rapid detection technologies such as RPA (recombinase polymerase amplification), LAMP (loop-mediated isothermal amplification) and the like.

The invention provides an interleukin 33 detection kit as well as a preparation method and application thereof. The interleukin 33 detection kit comprises a first antibody coupled to a magnetic fluorescent light-emitting microsphere, a second antibody labeled with biotin and fluorescein-labeled streptavidin, the first antibody strain and the second antibody strain are monoclonal antibodies for resisting interleukin 33, and interleukin 33 specific binding sites recognized by the first antibody strain and the second antibody strain are different. According to the kit disclosed by the invention, the interleukin 33 antigen is specifically combined with the magnetic fluorescent luminous microsphere formed by coupling the interleukin 33 capture antibody, then the magnetic fluorescent luminous microsphere is specifically combined with the biotin-labeled interleukin 33 detection antibody, the conjugate is combined with fluorescein-labeled streptavidin, fluorescence is emitted, and the content of the interleukin 33 in a sample to be detected is determined. The method is high in sensitivity, good in repeatability, short in reaction time, simple to operate and high in anti-interference performance.

The invention provides an indirect ELISA (Enzyme-Linked Immunosorbent Assay) antibody detection method and an indirect ELISA antibody detection kit based on pyruvate Kinase (PK), and relates to the technical field of parasite detection. The kit comprises a coating antigen Italiella mannici PK protein, a primary antibody, a labeled secondary antibody and an initiator solution, wherein the primary antibody is a mouse or cat anti-Italiella mannici PK protein antibody to be detected; the labeled secondary antibody can be combined with the primary antibody combined with the PK protein of the andrometrium mansoni; and the labeled second antibody is provided with a detection marker. The method comprises the following steps: cloning a PK protein gene into a prokaryotic expression vector PQE80L (+), constructing a recombinant plasmid PQE80L-PK, inducing PK recombinant protein expression by utilizing IPTG (isopropyl-beta-d-thiogalactoside), purifying the recombinant protein to coat an elisa plate, and establishing an indirect ELISA (enzyme-linked immuno sorbent assay) detection method of the Mangnocorynth PK protein antibody by optimizing reaction conditions. The detection method has the advantages of high sensitivity and strong specificity, and provides support for the development of the detection technology of the sitonia mansoni and the prevention and control of the schizocyst.

This invention relates to the field of biological detection, and more particularly to the preparation method and application of the t-PAI·C detection kit. The invention provides a detection reagent comprising: antibody-coated magnetic microparticles and enzyme-labeled antibodies; the antibodies include: tissue plasminogen activator-plasminogen activator inhibitor-1 complex antibody. In the detection reagent provided by this invention, when the sample contains the tissue plasminogen activator-plasminogen activator inhibitor-1 complex, the antibody coated with the magnetic microparticles and the enzyme-labeled antibody undergo an immune reaction with the complex, forming a sandwich-like immune complex consisting of the antibody coated with the magnetic microparticles, the complex, and the enzyme-labeled antibody; the enzyme on this complex acts on a luminescent substrate and emits light, and the concentration of the complex in the sample is calculated by detecting the luminescence signal.

The present invention proposes a microfluidic chip for the combined detection of C-reactive protein and ferritin and its application. The chip comprises a substrate and a cover sheet pressed onto the substrate. The substrate and cover sheet enclose a microchannel. The left end of the microchannel is connected to a buffer injection hole provided on the cover sheet, and the right end is connected to a waste liquid area. The waste liquid area is provided with a removable absorbent paper sheet. The microchannel is provided with a labeling area, a detection area, and a sample injection area from left to right. The labeling area is provided with a labeled anti-human CRP antibody, a labeled anti-human SF antibody, a labeled second antibody, and a labeled antigen. The first detection site is provided with an anti-human CRP antibody, the first reference point is provided with a first antibody that reacts with the labeled second antibody, the second detection site is provided with an anti-human SF antibody, and the second reference point is provided with an antibody that reacts with the labeled antigen. This can simplify the detection process of C-reactive protein and ferritin and shorten the detection time.

The invention relates to a synthesis method of an antibody-drug conjugate labeled by a position-selective antibody based on proximity effect. According to the present application, it is possible to provide a method for synthesizing an antibody-drug conjugate by introducing a drug position-selectively into an antibody using a pyridine oxime derivative (labeled mediating protein) introduced into a binding protein comprising a non-standard amino acid.

The application discloses a pet inflammation multi-channel saliva detection test strip combined pad, a method and application, and particularly relates to the technical field of biomedical detection. The combined pad comprises a porous matrix, a polydopamine (PDA) bottom layer formed on the surface of the matrix, a poly(N,N-diethylaminoethyl methacrylate) (PDEAEMA) brush polymer layer grafted on the surface of the PDA bottom layer, and an antibody-labeled nanoparticle conjugate loaded in the polymer layer. The PDEAEMA brush polymer layer undergoes conformational change within the saliva pH range, shields and protects the antibody-labeled nanoparticle conjugate under higher pH conditions, and releases the antibody-labeled nanoparticle conjugate to participate in lateral chromatography under lower pH conditions. The multi-channel saliva detection test strip based on the combined pad forms multiple independent detection zones through a hydrophobic isolation structure, and is used for parallel detection of different inflammation markers.

The present invention discloses a platelet-based immune activation system, its preparation method, and its application, belonging to the field of biomedicine. Using platelets as carriers, the system engineered antibodies and cytokines onto the platelet surface. Leveraging the platelets' natural targeting properties to postoperative wounds and tumor adhesion, the antibodies achieve universal and stable antibody labeling of postoperative tumors. Furthermore, the precisely released cytokines fully mobilize immune cells in the tumor microenvironment, promoting their recognition and killing of residual tumors, thereby inhibiting postoperative tumor recurrence and metastasis.

The invention is applicable to the technical field of in-vitro diagnosis, and provides a centrifugal-lateral flow immune micro-fluidic chip for combined detection of allergens and a component sIgE, the centrifugal-lateral flow immune micro-fluidic chip comprises a chip body, and the chip body is fused with a centrifugal micro-fluidic functional area and a lateral flow micro-fluidic functional area; according to the method, an indirect immunoassay mode of firstly adding a specimen, capturing a to-be-detected antibody by an antigen probe to form a compound, capturing the compound by a microfluidic detection unit streptavidin, then dissolving a labeled antibody, combining the labeled antibody with the antigen probe and obtaining the to-be-detected antibody compound is adopted; the reaction is firstly completed in the reaction unit and then enters the lateral flow channel, at least two allergens / components can be synchronously detected, the sample dosage is less than or equal to 10 microliters, the detection time is less than or equal to 30 minutes, the sensitivity is improved by more than 10 times compared with that of a traditional method, and the method is adaptive to grassroots, emergency treatment and children screening scenes.

The present invention provides a rapid, non-invasive, and antibody-free method for identifying AA and MDS based on Raman spectroscopy. This method utilizes the peak intensity of Raman spectra as a biomarker for the preparation of products for predicting or detecting blood disorders. The specificity and significant Raman shifts of the present invention are valuable biomarkers for early disease identification, improving diagnostic efficiency, reducing testing costs, and promoting the rapid and accurate diagnosis of AA and MDS.

The invention relates to the technical field of biological detection, and discloses a salmonella monoclonal antibody, a salmonella colloidal gold immunochromatography test strip based on the antibody and a preparation method of the salmonella colloidal gold immunochromatography test strip. Salmonella is used as an antigen, a polyclonal antibody and a monoclonal antibody are prepared respectively, the monoclonal antibody 8E71C8 is screened out, and the salmonella colloidal gold immunochromatography test strip is prepared. The purified monoclonal antibody is used as a 30 nm colloidal gold marker, the optimal pH and the optimal antibody labeling amount of binding of the monoclonal antibody and colloidal gold and the concentration of the polyclonal antibody used as a detection line are optimized, the prepared salmonella colloidal gold immunochromatography test strip is high in specificity, the sensitivity is about 1 * 10 < 4 > CFU / mL and is higher than the average detection lower limit of domestic similar products, and the salmonella colloidal gold immunochromatography test strip can be used for detecting salmonella. And the test strip is simple to operate and high in efficiency, can be used as a means for rapidly and visually detecting the salmonella, and has popularization and application values.

The present application relates to the technical field of biology, and particularly relates to a preparation method and application of an anti-oncofetal antigen monoclonal antibody. The present application discloses a monoclonal antibody 12# with a heavy chain variable region shown in SEQ ID NO:1 and a light chain variable region shown in SEQ ID NO:2. The present application further discloses a preparation method and application of the antibody. The present application uses CEA extracted from liver cancer tissue after liver metastasis of intestinal cancer as an immunogen to immunize Balb / c mice, and uses hybridoma technology to screen and purify 12 strains of anti-CEA monoclonal antibodies. The obtained antibodies are labeled with horseradish peroxidase, and through cross pairing, one strain of antibody that can be used for labeling is selected and applied to detect CEA in human serum. The double antibody sandwich detection method established by using the labeled antibody has the advantages of high sensitivity, simple operation, short detection time and the like, and can provide key materials for subsequent development of commercial kits for CEA detection.

The application discloses a kind of DOC-Chaps synergistic core's clinical and animal tissue rapid transparent reagent and three-dimensional imaging method. Rapid transparent reagent is mainly dissolved in deionized water by sodium deoxycholate (DOC), 3-[(3-cholamide propyl) dimethylamino]-1-propane sulfonic acid (Chaps), urea and 1-methylimidazole; DOC efficiently dissolves high-density lipid, Chaps gently destroys membrane structure, both complement each other, realize lipid rapid, depth clearance and significantly reduce light scattering; urea loosens collagen fiber and protein aggregate, improves the penetration rate of reagent; 1-methylimidazole selectively removes hemoglobin, reduces light absorption. The transparent reagent of the application can make millimeter thick brain slices in 4 (mouse brain) / 6 (human brain) hours The light transmittance of specific wavelength is greater than or equal to 90%, and there is no obvious tissue shrinkage / expansion, the compatibility of fluorescent protein and antibody label is excellent, suitable for three-dimensional imaging of high-lipid, high-hemoglobin samples such as in-vitro human brain, kidney, liver and mouse brain, heart, kidney, liver, intestinal tract, embryo, etc.

The invention relates to the technical field of pesticide residue detection, in particular to a preparation method of a cyhalothrin colloidal gold quantitative test strip, and solves the technical problems of non-uniform antibody labeling, coating layer cracking and low detection sensitivity in the traditional process. Taking 1 ml of 40-nanometer colloidal gold particles, adding potassium carbonate, mixing and reacting for 5-15 minutes, then adding 5-7 micrograms of cyhalothrin monoclonal antibody, reacting, and finally adding confining liquid to prepare a colloidal gold labeled antibody solution; spraying the obtained solution on a glass fiber pad according to the spraying amount of 9.0-10.8 microliters per centimeter to prepare a gold-labeled pad; a specific coating buffer solution is used for preparing a quality control line solution and a detection line solution of 0.7-0.9 mg / ml cyhalothrin-BSA antigen, a nitrocellulose membrane is coated with the quality control line solution and the detection line solution, and after confining liquid treatment, centrifugation and resuspension, the nitrocellulose membrane is dried for 70-74 hours in inert gas with the temperature of 45 DEG C and the temperature of 2 DEG C; the test strip prepared by the method is used for high-precision quantitative detection of cyhalothrin residues in agricultural products.

The invention provides an outer vesicle surface protein in-situ labeling method, and belongs to the technical field of outer vesicles, and the outer vesicle surface protein in-situ labeling method provided by the invention comprises the following steps: carrying out differential centrifugal separation on outer vesicles, carrying out quality evaluation by adopting nano-particle tracking analysis, and establishing a positive and negative correlation coupling equation set to optimize labeling conditions. The method comprises the following steps: fixing a sample by using glutaraldehyde, labeling antibodies in an optimized labeling buffer solution, removing free antibodies through ultrafiltration centrifugation, carrying out singular value decomposition calculation, adding a special protective agent, analyzing morphological characteristics by adopting a deep learning model, and ensuring the stability of the sample through filter membrane filtration and low-temperature preservation. In the whole process, a complete quality control system is established, the labeling targets of high specificity, high efficiency and high stability are achieved, and the technical problems of low labeling specificity and poor stability in the in-situ labeling process of the outer vesicle surface protein in the prior art are solved.

The invention provides a method for removing background fluorescence of a tissue sample, which comprises the following steps: mixing the tissue sample with a metal hydride reducing agent, and illuminating the tissue sample under white light and a light source with the wavelength of 475-485 nm. According to the method, the background fluorescence of the tissue sample can be obviously removed, the background is cleaner after the treated tissue is subjected to antibody dyeing, the strength and expression quantity of an antibody labeling signal are not influenced, the signal-to-noise ratio of a specific fluorescence labeling signal is high, the removal effect is thorough, and the background fluorescence cannot be recovered. Meanwhile, the method disclosed by the invention is low in cost and simple and convenient to operate, and has a wide application prospect in technologies such as immunofluorescence analysis and the like.

The invention discloses a large-volume sample fluorescence labeling method based on tissue fixation. The fluorescence labeling method comprises the following steps: a sample fixing step: fixing a biological tissue sample by adopting paraformaldehyde; and a fluorescence labeling step: placing the biological tissue sample subjected to fixation treatment in a stationary liquid containing glyoxal, and realizing fluorescence labeling of the biological tissue sample by utilizing autofluorescence generated by browning due to fixation of glyoxal. According to the fluorescent labeling method, fluorescent dye and antibody labeling are not needed, labeling of cell bodies and fiber bundles can be achieved in a non-degreasing state, and meanwhile blood vessel information can be obtained through a contrast means; moreover, the method is easy to process, the sample can be rapidly and uniformly permeated, the sample processing time is greatly shortened, and a new way is opened up for obtaining three-dimensional structure information of different large sample tissues and organs at the submicron scale.

This invention relates to the field of biological detection, and more particularly to antibody combinations and their application in the preparation of detection products for TPS. The monoclonal antibodies provided in this invention are named 7#, 13#, and 16#. This invention also discloses the preparation method and application of these antibodies. This invention uses prokaryotically recombinantly expressed CK18 as an immunogen to immunize mice. Sixteen anti-TPS monoclonal antibodies were obtained through screening and purification using hybridoma technology. The obtained antibodies were labeled with horseradish peroxidase. Epitope identification was performed on the synthesized peptides, and cross-pairing of different epitope antibodies was used to select one antibody suitable for coating and two antibodies suitable for labeling. These were then applied to detect TPS in human serum. The double-antibody sandwich detection method established using the above antibodies can be applied to a magnetic microparticle chemiluminescence platform, and has the advantages of high sensitivity, simple operation, and short detection time. It can provide key materials for the subsequent development of commercial kits for TPS detection.

The invention discloses an application of FXYD1 protein in sperm centromere detection. The amino acid sequence of the FXYD1 protein is as shown in SEQ ID NO. 1. By identifying an antibody of the FXYD1 protein, the expression position of the FXYD1 protein on the sperm is marked, and the number and position of centromere in the sperm are analyzed by combining immunostaining and microscope observation. The method can effectively evaluate the sperm centromere structure state, including existence, number and position form of centromere; the related expression of the sperm FXYD1 protein can also be evaluated. The human sperm quality evaluation method provided by the invention is helpful for objectively judging sperm functions, embryonic development after fertilization, physiological and pathological states related to male fertility and prognosis results of the physiological and pathological states, and has important transformation and popularization values and wide application prospects in the fields of reproductive biology and reproductive medicine.

The invention discloses a method for detecting quinoxaline antibiotics and main metabolites thereof, and relates to the field of agriculture and forestry chemistry detection.The method comprises the following steps that 1, a UCNPs-mAb fluorescent probe is prepared; step 2, preparing and assembling UCNPs-LFSBs; step 3, optimizing a UCNPs-LFSBs system, wherein the optimization content comprises the antibody labeling amount, the concentration of OLA-OVA, QCT-OVA and MQCA-BSA on a T line, the concentration of GAM-IgG on a C line, the mixing ratio of UCNPs-mAb mixed probes on a conjugate pad, the artificial antigen membrane scratching position, a buffer solution and the reaction time; by means of the optimized detection method, OLA, QCT and MQCA can be rapidly and quantitatively measured at the same time with high sensitivity.

The invention discloses a method for screening umbilical cord mesenchymal stem cells with an anti-depression function and a therapeutic drug. The method comprises the following steps: acquiring an umbilical cord mesenchymal stem cell sample; a CD146-PE antibody is used for marking sample cells, and flow cytometry is adopted for detection; determining the proportion of CD146 antibody-labeled positive cell subgroups in the sample; when the proportion is greater than 50%, the sample is judged to have an anti-depression function, and the effect of the sample in treating depression is predicted to be remarkable. The method can be used for efficiently screening the umbilical cord mesenchymal stem cell subpopulation for relieving depression, and has important medical application value and economic value.

The present invention discloses a lyophilized protective agent composition for AlphaLISA detection and a preparation method thereof. The method comprises: diluting a biotinylated antibody and an antibody-labeled receptor bead with a diluent to a desired concentration, mixing the two solutions to obtain a detection reagent A, and diluting a streptavidin-coupled donor microsphere with a diluent to a desired concentration to obtain a detection reagent B; adding a lyophilized protective agent to A and B respectively, and mixing them evenly; transferring the reagents to a bead dropper, quick-freezing the reagents in liquid nitrogen to form ice balls, and vacuum-drying the quick-frozen reagent balls with liquid nitrogen to obtain freeze-dried beads of detection reagents A and B. After adding the lyophilized protective agent and using liquid nitrogen for quick freeze drying, the freeze-dried reagents are in the form of small balls with a good round effect, uniform shape and volume, consistent size, low moisture content, and can be stored stably for a long time without affecting the detection signal value, and the time required is shorter than that of conventional freeze drying.