174 results about "Reproductive medicine" patented technology

An integrated microfluidic device and its usage are provided. The microfluidic device comprises an upper layer (1) and a lower layer (2), wherein the lower layer (2) is bound to the upper layer (1). The upper layer (1) comprises a micro-channel (3) and the lower layer (2) comprises a micro-well (7) array. The micro-channel (3) is in fluidic connection with the micro-well (7) array, and the height of the micro-channel (3) is greater than the diameter of the oocyte (4) flowing through the micro-channel (3). The integrated microfluidic device has many advantages including low cost, high integration, and convenient operation, and has application prospects in reproductive medicine and the research of fertilization and embryo early development.

The invention belongs to the fields of gene engineering and reproductive medicine, and discloses a single nucleotide polymorphism (SNP) marker related with clinically cryptogenic non-obstructive azoospermia aided diagnosis and application of the SNP marker. The marker is a combination of rs1014331, rs10765875, rs10889203, rs11066453, rs11167809, rs12097821, rs1990264, rs3000811, rs3130048, rs391129, rs4716515, rs658239, rs6589102, rs7042232, rs7099208, rs7144703, rs7194 and rs985421. The marker can be used for preparing a clinically cryptogenic non-obstructive azoospermia aided diagnosis kit.

The invention which belongs to the medical field of genetic engineering and reproduction discloses a seminal plasma miRNA marker associated with human non-obstructive azoospermia and an application thereof. The maker is selected from several of hsa-miR-141, hsa-miR-193a-5p, hsa-miR-590-5p and hsa-miR-7-1*. The maker which has specificities and sensitivities to the non-obstructive azoospermia can be used for the preparation of a reagent for non-obstructive azoospermia diagnosis or monitoring, so invasive diagnosis is avoided, repeated detection is realized, and the dynamic monitoring of the obstacle degree of sperm generation can be easily achieved.

The present invention relates generally to the fields of reproductive medicine. More specifically, the present invention relates to a method determining the developmental stage of human cumulus cells issues from MII oocyte.

The invention belongs to the field of gene engineering and reproductive medicines, and discloses a seminal plasma micro ribonucleic acid marker related to spermatogenesis deficiency and application thereof. The marker is selected from one or more of SEQ ID No.2, SEQ ID No.3 and SEQ ID No.4, has specificity and sensitivity to the spermatogenesis deficiency, can be used for preparing a reagent for diagnosing or monitoring the spermatogenesis deficiency, can avoid invasive diagnosis, can carry out repeated detection, and is easy to dynamically monitor the degree of the spermatogenesis deficiency.

The invention discloses a novel low-temperature preservation device for reproductive medicine. The device comprises a refrigeration tank and a tank cover; the tank cover is matched with the refrigeration tank; liquid nitrogen is arranged in refrigeration tank; the inner bottom wall of the refrigeration tank is fixedly connected with a fixing plate; a guide rod is fixedly connected to the left sideof the top of the fixing plate; a left sliding plate is slidably connected to a guide rod; a screw rod is rotatably connected to the right side of the top of the fixing plate; a right sliding plate is screwed to the screw rod; a test tube placement rack is arranged between the left sliding plate and the right sliding plate; a control assembly is arranged at the top of the fixing plate; and a damping moving assembly is arranged at the bottom of the refrigeration tank. The test tube placement rack is adopted, so that a plurality of specimens can be simultaneously stored; the position of the test tube placement rack can be adjusted by rotating the screw rod, so that the specimens are very convenient to place and take; whether the specimens are located on the lower portion in the refrigeration tank or not can be known through the control assembly, so that the preservation effect of the specimens is good; the damping moving assembly is arranged, a damping effect can be achieved when the device moves, and so that the test tubes storing the specimens are prevented from being damaged.

The invention discloses a vagina washing device used for the reproductive medicine department. The vagina washing device comprises a rotation outer barrel and a fixed inner barrel, wherein a pluralityof liquid spray holes are formed in a side plate of the rotation outer barrel at equal intervals, the fixed inner barrel is fixedly mounted on the upper surface of the middle of a support baffle ring, the rotation outer barrel is rotatably mounted on the support baffle ring in an erecting manner, a screw rod is rotatably arranged on the central axis of the support baffle ring, a driving gear is fixedly mounted at the outer end of the part, placed in the rotation outer barrel, of the screw rod, an annular rack is fixedly mounted on the inner wall of the upper inner cavity of the rotation outerbarrel, and a piston assembly is arranged in the fixed inner barrel in a vertical sliding manner. The vagina washing device is simple in structure and convenient to use, the comprehensive washing forthe vagina can be realized, the washing dead angle can be avoided, thus the washing effect is good, and the use is safe and sanitary.

The invention belongs to the field of gene engineering and reproductive medicine, and discloses mitochondria-related seminal plasma miRNAs taken as mankind severe asthenospermia markers, and applications thereof. The markers are mitochondria-related miRNAs selected from hsa-miR-101-3p, hsa-miR-151a-5p, and hsa-let-7b-5p. The markers can be used for separating patients with severe asthenospermia from a control group with normal fertility, and can be used for preparing human severe asthenospermia diagnosis or monitor kits.

The invention belongs to the fields of genetic engineering and reproductive medicine and discloses an SNP marker of mitochondria DNA related to asthenospermia with unknown clinical causes and application thereof. The marker is a combination of C5601T, T12338C, Al2361G, G13928C, Al5851G, C16179T and G16291A, can be used for preparing an auxiliary diagnosis kit for asthenospermia with unknown clinical causes and has high specificity and sensitivity.

The invention belongs to the field of biological engineering and provides a method for separating human spermatogonial stem cells. The method comprises the following steps of: mechanically separating a testicular tissue; processing the testicular tissue into a semi-liquid state; adding digestive enzyme solution I into the liquid testicular tissue in the semi-liquid state; digesting the testiculartissue into a single convoluted tubule; adding digestive enzyme solution II in the convoluted tubule; digesting the convoluted tubule into a single cell; separating reproductive cells from supportingcells in the single cell; separating G protein-coupled receptor 125 (GPR125) positive spermatogonial stem cells from glial cell line-derived neurotrophic factor (GDNF) family receptor alpha 1 (GFRA1)positive spermatogonial stem cells by adopting an immunomagnetic bead cell sorting method; and identifying the separated GPR125 positive spermatogonial stem cells and the GFRA1 positive spermatogonial stem cells by adopting an immune cell chemical method. By the method, a large number of the high-purity human spermatogonial stem cells are obtained, and sufficient cell sources are provided for theresearch of the human spermatogonial stem cells and the application of the human spermatogonial stem cells in regenerative medicine and reproductive medicine.

The invention relates to the field of reproductive medicines, in particular to an ovarian tissue cryopreservation method. The ovarian tissue cryopreservation method comprises the following steps: culturing an ovarian tissue to be frozen by a hyaluronidase solution with the volume concentration of 8-12 percent for 10-15 minutes so as to obtain an enzymolyzed ovarian tissue; adding the enzymolyzed ovarian tissue into a freezing protection agent for prebalancing under the temperature of 3-5 DEG C for 10-15 minutes to obtain a prebalanced ovarian tissue; performing programmed cryopreservation on the prebalanced ovarian tissue to reduce the temperature of the prebalanced ovarian tissue to be minus 150 DEG C, and transferring the prebalanced ovarian tissue into a liquid nitrogen tank for storage. Compared with the common prebalancing time of 30-90 minutes in the prior art, the prebalancing time of the ovarian tissue cryopreservation method is greatly shortened, so that the cytotoxic damage, which is caused by the freezing protection agent, to the ovarian tissue under overlong prebalancing time is avoided.

The invention discloses a traditional Chinese medicine patch for non-invasive detoxification of the reproductive system and a production method thereof, in which an adhesive layer protective layer, an adhesive layer, an anti-seepage layer, a moisture absorption layer, a traditional Chinese medicine essence layer and a skin-friendly layer are sequentially arranged from bottom to top , and then heat-pressed, glued and die-cut, sealed and packaged in a single medical aluminum foil bag, and sterilized by radiation. The Chinese medicine essence layer is non-woven fabric wrapped with Chinese medicine. The Chinese medicine is Tianshan Snow Lotus with a weight percentage of 8%. , 17% asparagus, 14% astragalus, 16% cnidium, 21% Kochia scoparia, 12% flavescens, 4% borneol, and 8% safflower. The system has the characteristics of non-invasive detoxification, good effect, and convenient production and use.

The present invention relates to a new biomarker for the medicine and the biology of reproduction, in particular for in vitro fertilization (IVF) outcome. It relates to methods for predicting IVF outcome and for selecting the subject for IVF.

The invention relates to a clinically unexplained NOA (non-obstructive azoospermia)-related mitochondrial DNA SNP (single nucleotide polymorphism) marker and applications thereof, belonging to the fields of genetic engineering and reproductive medicine. The marker is a combination of the following mitochondrial DNA SNP sites: T3394C, A6881G, G11696A, G13135A, G13928C, A15662G, T15968C, T16224C and G16319A. The marker and specific primers and / or specific probes thereof can be used for auxiliary diagnosis of the clinically unexplained NOA, greatly improving the sensitivity and specificity of diagnosis.

The invention discloses an artificial insemination transplantation device for reproductive medicine, and belongs to the field of the reproductive medicine. The artificial insemination transplantationdevice comprises a sperm storage portion and a force applying portion; the sperm storage portion comprises a sperm storage cylinder, an airbag sleeve is slidably mounted on the outer side of the middle of the sperm storage cylinder, a sperm outlet hole is formed in the middle of the top end of the sperm storage cylinder, and a moving plug is mounted in the sperm storage cylinder; the force applying portion comprises a force applying cylinder, a fixing rod is mounted in the middle of the lower end of the force applying cylinder, a grip rod is mounted on the left side of the lower end of the fixing rod, a rotating rod is connected to the middle of the right end of the fixing rod through a hinge rod, a stop plate is mounted at the bottom end of the force applying cylinder, an air outlet checkvalve is mounted in the middle of the stop plate, a pressing airbag is connected to the bottom end of the stop plate, an air inlet tube is mounted in the middle of the bottom end of the pressing airbag, and an air inlet check valve is mounted in the middle of the air inlet tube. The artificial insemination transplantation device has the advantages that the situation that the sperm storage portioncannot be used when the force applying portion is broken is avoided; through cooperation between the rotating rod and the pressing airbag, a single person can complete air inflation and sperm pushingsimultaneously, so that time and labor are saved, and practicality and reliability are high.

The present invention relates generally to the fields of reproductive medicine. More specifically, the present invention relates to methods and kits for determining the human sperm quality.

The invention belongs to the field of genetic engineering and reproductive medicine and discloses an SNP marker related to auxiliary diagnosis of non-syndromic congenital heart disease and an application thereof. The SNP marker is composed of rs3789612, rs4140836, rs13391263, rs13101737, rs1400558, rs6872396, rs13189951, rs9266596, rs7863990, rs2433752, rs7259736, rs490514, rs2474937, rs1531070, rs9533839, rs17019472, rs10182004, rs9461938, rs72759270 and rs1886441. The marker can be used for preparing an auxiliary diagnosis kit of the non-syndromic congenital heart disease.

The invention belongs to the field of genetic engineering and reproductive medicine and discloses a matrix serum / plasma miRNA (Micro Ribonucleic Acid) marker associated with fetal congenital heart diseases and an application of the marker. The marker is the combination of several of mi-19b, mir-22, mir-29c and mir-375. The marker can be used for well separating cases of fetal congenital heart diseases from healthy controls and can play a good supporting role in early diagnosis of fetal congenital heart diseases.

The invention relates to a symbolic micro RNA marker for detecting a spermatogenic failure passage effect and application thereof, belongs to the field of biotechnology and reproductive medicine, and discloses a micro RNA (miRNA) marker for detecting a spermatogenic failure passage effect and application thereof. The marker is a plurality selected from a group consisting of rho-miR-1-3p, rho-miR-124-3p and rho-miR-190a-5p. The marker has specificity and sensitivity for monitoring the spermatogenic failure passage effect, provides a reliable detection basis and quick detection method for diagnosis monitoring of the spermatogenic failure passage effect and has important application values.

The invention discloses a GRP78 gene SNP (Single Nucleotide Polymorphism) marker related to asthenospermia, and application thereof, and relates to the technical field of genetic engineering and reproductive medicine. A multiple single base extension technology is adopted to analyze the correlation of the polymorphism of a plurality of locus of the GRP78 gene and the asthenospermia to obtain thatGRP78 genes rs3216733 and rs391957 SNP have correlation with the occurrence of the asthenospermia, protein expression can be lowered to increase an asthenospermia occurrence risk, and the GRP78 gene SNP marker can be used as reagent or a kit for assisting in diagnosing the asthenospermia.

The invention provides an intelligent seminal fluid optimization system and relates to the field of reproductive medicine equipment. The system comprises a centrifugation device as well as a sucking loading / unloading system, a precise liquid supply device and a liquid sucking device , wherein the sucking loading / unloading system, the precise liquid supply device and the liquid sucking device correspond to the working position of the centrifugation device; the sucking loading / unloading system comprises a seminal fluid bottle supply device, a to-be-loaded test tube supply device, an unloaded test tube conveying device and a sucking loading / unloading mechanical arm which is operated along a sucking loading / unloading track; the sucking loading / unloading mechanical arm is used for quantitatively sucking a seminal fluid in a seminal fluid bottle into a to-be-loaded test tube and used for conveying the to-be-loaded test tube to the working position of the centrifugation device, or used for conveying a centrifuged test tube to the unloaded test tube conveying device; the precise seminal fluid supply device is used for injecting a liquid into the to-be-loaded test tube; and the liquid sucking device is used for sucking a supernate from a test tube from a preset height. By adopting the intelligent seminal fluid optimization system, automatic proportioning of the seminal fluid with the liquid can be achieved, automatic loading / unloading of test tubes can be achieved, and the efficiency can be greatly improved.

The invention discloses a constant temperature and pressure vagina cleaning device for reproductive medicine. The device comprises a collecting pipe, an electric heater is arranged at a mounting holeformed in the right end face of the collecting pipe, a handle is fixed to the upper side face of the collecting pipe, and a storage battery and a control switch are installed on the handle. The constant temperature and pressure vagina cleaning device for reproductive medicine is simple in structure and convenient to use, a cleaning solution is introduced through a liquid conveying pipe to clean the vagina of a patient, the cleaning solution is heated through an electric heater, the phenomenon that due to the fact that the temperature of the cleaning solution is too low, an irritative reactionis generated is avoided, and the cleaning solution sprayed by a branch pipe makes contact with vaginal tissue, bypasses an elastic ring and flows back; in the process, the vaginal wall is cleaned, waste liquid obtained after cleaning is collected and uniformly treated through a waste pipe, pollution and cross infection are reduced, the deformable branch pipe performs elastic limit on fluctuation of cleaning solution fluid, the stability of cleaning pressure is guaranteed, convenience is brought to overall operation, and the cleaning efficiency is high.

The invention provides novel compositions and compounds that inhibit CatSper channel activity, that preferentially inhibits sperm hyperactivity over sperm motility, or both. The compounds of the invention are useful as contraceptive agents that may be adminstered to males, females, or concurrently to both sexual partners. The invention further provides methods of conducting drug discovery business and of conducting a reproductive medicine business. The invention also provides methods of identifying compounds that modulate sperm hypermotility.

The invention provides novel compositions and compounds that inhibit CatSper channel activity, that preferentially inhibits sperm hyperactivity over sperm motility, or both. The compounds of the invention are useful as contraceptive agents that may be adminstered to males, females, or concurrently to both sexual partners. The invention further provides methods of conducting drug discovery business and of conducting a reproductive medicine business. The invention also provides methods of identifying compounds that modulate sperm hypermotility.

The present invention relates generally to the fields of reproductive medicine. More specifically, the present invention relates to in vitro non-invasive methods for determining the quality of an embryo by determining the level of the cell free nucleic acids or miR-29a or let7-b in the nucleic acid extract from a follicular fluid sample.

The invention discloses a method for establishing a source of heredofamilial premature ovarian failure and inducing differentiation of totipotential stem cells to original genital ridge cells. The method is characterized in that adult cells of patients with heredofamilial premature ovarian failure are applied and reprogrammed into induced totipotential stem cells in vitro, and then are induced and differentiated into precursor cells of the oocytes, namely the original genital ridge cells. The method is a method for obtaining original genital ridge cells from patients with heredofamilial premature ovarian failure, lays foundation for further obtaining manure oocytes, and is expected to be applied in the field of reproductive medicine and translational medicine.

The invention discloses a low-temperature preservation device for reproductive medicine. The low-temperature preservation device comprises a low-temperature preservation device body, a sealing door, atransparent observation device and a purification device, wherein the front part of the low-temperature preservation device body is connected with the sealing door through a rotating shaft; the middle of the sealing door is embedded with the transparent observation device; the top of the transparent observation device is provided with a temperature display screen; lock heads are distributed on the top of the inner side of the sealing door; lock holes are distributed at the bottom of the front side of the low-temperature preservation device body; the interior of the low-temperature preservation device body is provided with a reproductive medical article placing cavity; a partition net is arranged in the middle of the interior of the reproductive medical article placing cavity; temperaturesensors are distributed on the left side of the interior of the reproductive medical article placing cavity; and a purification device is arranged on the top of the low-temperature preservation devicebody. By using the low-temperature preservation device for reproductive medicine, reproductive medical articles inside the device can be directly observed, and the low-temperature preservation deviceis efficiently mounted and fixed, can efficiently filter impurities, dust and peculiar smells, and can realize disinfection and purification in real time.

The invention discloses a constant-temperature vagina cleaning system used in the reproductive medicine department. The constant-temperature vagina cleaning system comprises a cleaning device body, wherein a cleaning fluid storage tank is arranged at one end of the cleaning device body, a control box is arranged at the other end of the cleaning device body, a cover plate is arranged at the front end of the cleaning fluid storage tank and is connected with the cleaning fluid storage tank through a hinge, a plurality of cleaning fluid containers are arranged inside the cleaning fluid storage tank, a connector is arranged at the upper end of each cleaning fluid container, a plurality of cleaning fluid water suction pumps are arranged at the upper end of the cleaning fluid storage tank and are in threaded connection with the cleaning fluid containers through the connectors, and the cleaning fluid water suction pumps are connected with vagina cleaning heads through cleaning fluid output pipes. The constant-temperature vagina cleaning system is simple in structure, cleaning fluids containing different components or different in concentration can be optionally blended according to suggestions of doctors, the water amount for each cleaning fluid can be optionally adjusted during cleaning, the inside of the vagina can be automatically and deeply cleaned, a cleaning effect is improved, and bacterial infection is avoided.