31 results about "Follicular fluid" patented technology

The primary culture method of the porcine ovary granular cells comprises the following steps: step 1, taking a fresh porcine ovary, quickly putting the porcine ovary into a Hank's balanced salt solution preheated at 38 DEG C for transportation, repeatedly washing the ovary with the Hank's balanced salt solution to remove blood and impurities, and then extracting follicular fluid with a 5ml syringe under a sterile condition; according to the method, the granular cells can be effectively separated and cultured from the porcine ovary, and the porcine ovary granular cells with higher purity and better activity can be obtained, so that the subsequent cell biology experiment research, such as gene transfection, protein expression analysis and the like, is facilitated; a cell model is provided for researching the physiological functions (such as hormone secretion and follicle development regulation and control) of the porcine ovarian granular cells, the operation is relatively simple, the cost is relatively low, and the used reagents and instruments are common in a cell culture laboratory and are convenient to popularize and apply.

The utility model discloses an in-vitro follicular fluid semi-automatic collecting device which comprises a negative pressure adsorption assembly, the negative pressure adsorption assembly comprises a portable liquid suction device, a waste liquid cup is detachably arranged on one side of the portable liquid suction device, the portable liquid suction device is connected with the waste liquid cup through a suction pipe, and the suction pipe is connected with the negative pressure adsorption assembly. A suction catheter is arranged at the upper end of the waste liquid cup, one end of the suction catheter extends into the waste liquid cup, and the other end of the suction catheter is fixedly connected with a three-way valve; and the connecting pipe group comprises an exhaust extension hose. According to the in-vitro follicular fluid semi-automatic collecting device, the negative pressure adsorption assembly, the connecting pipe set and the sampling needle set are combined, follicular fluid can be extracted in a semi-automatic mode, a large amount of follicular fluid needs to be collected, for example, when the follicular fluid exceeds 200 mL, the follicular fluid can be collected by multiple persons through the same instrument at the same time, materials are used as least as possible, and the cost is reduced. The sampling speed is increased, and the follicular fluid collection efficiency and quality are guaranteed.

Microfluidic systems are configured to process liquid samples that include cumulus-oocyte complexes (COCs), such as raw follicular fluid, in an automated and continuous manner to produce oocytes separate or “denuded” from surrounding cumulus cells. The systems include a substrate and a channel that can have two or more sub-channels. Each channel includes an inlet, an outlet, and one or more stages arranged in series. Each of the stages includes one or more expansion units and one or more constriction units. At least one stage includes constriction units having jagged internal surfaces, e.g., teeth, that help remove the cumulus cells from the COCs.

The application belongs to the technical field of biology and particularly relates to application of phosphatidylglycerol. The phosphatidylglycerol PG (20:4_22:6) is used as a biomarker, and through analysis on metabolites of follicular fluid, the application realizes diagnosis of decreased ovarian reserve (DOR) with high sensitivity, high specificity and high accuracy.

The invention discloses application of the ratio of phosphatidylserine to taurocholic acid. Research finds that compared with assisted reproduction crowds capable of obtaining available embryos after embryo culture, the concentration of phosphatidylserine in follicular fluid of assisted reproduction crowds without available embryos after embryo culture is remarkably reduced, which prompts that phosphatidylserine can be used as a marker for predicting developmental potential of assisted reproduction embryos; the prediction module is used for predicting whether available embryos can be obtained or not in advance in an egg taking stage of assisted reproduction; and the ROC curve verifies that the AUC of the PS (21: 220: 4) is 0.741, and the AUC of the ratio of the PS (21: 220: 4) to the taurocholic acid is 0.784, so that the accuracy and the sensitivity are higher when the ratio of the PS (21: 220: 4) to the taurocholic acid is used as the marker to predict whether available embryos exist or not.

The invention relates to the technical field of animal nutrition and reproduction, and discloses application of arginine in luteinization of replacement gilts. The arginine is used as a nutritional supplement for regulating and controlling the hormone level of the replacement gilts, the progesterone content can be effectively increased, and corpus luteum generation is promoted. Meanwhile, after the porcine ovarian granular cells are cultured, arginine can up-regulate expression of genes related to synthesis of progesterone and down-regulate expression of genes related to synthesis of estrogen, so that the progesterone is increased, the estrogen is reduced, and the effects of increasing the generation quantity of corpus luteum and maintaining a good pregnancy environment are achieved. According to the invention, 0.5% of arginine is directly added into the pig daily ration, so that the progesterone content in follicular fluid and serum can be remarkably increased, the estrogen content can be reduced, the quantity of small corpus luteum can be increased, and the pregnancy quality and success rate of the replacement gilts after mating can be improved. According to the invention, the problem of hormone level regulation can be solved, and a new nutrition regulation target is provided for increasing the luteum generation of the replacement gilts and improving the reproductive performance of the gilts.

The utility model discloses a multifunctional cattle live ovum collection follicular fluid collecting tube relates to the technical field of veterinary medical instruments, including transparent tube, the outer wall of transparent tube is equipped with scale, the inner chamber fixed connection of transparent tube has the partition board, the top threaded connection of transparent tube has the pipe cover, the inside of pipe cover is provided with adjusting assembly, the inside of pipe cover is provided with positioning assembly. The utility model discloses through knob, transparent silica gel tube and positioning assembly cooperation, has reached the effect of real -time identification and accurate shunt, when observing that the transparent silica gel tube appears red blood, will knob rotate 120, drive transparent silica gel tube synchronous rotation, when rotating a certain distance, by positioning assembly to the positioning of fixed frame, the transparent silica gel tube aims at the matching chamber, so that the subsequent blood can be arranged into the matching chamber, avoids sample cross -contamination.

The invention relates to the technical field of livestock breeding biology, and discloses a method for promoting porcine ovary granular cell proliferation, which comprises the following steps: adding a porcine follicular fluid exosome into a porcine ovary granular cell culture system, and acting for a certain time at an effective concentration, so as to improve the proliferation activity of granular cells. After the exosome treatment, the apoptosis rate of granular cells can be reduced, the cell survival rate can be improved, and the granular cells can be promoted to secrete steroid hormones, including the estradiol level and the expression of progesterone synthetase. Cell experiments prove that the follicular fluid exosome can effectively improve the activity of granular cells, promote the process of a cell cycle, inhibit cell apoptosis and remarkably enhance the synthesis capacity of steroid hormones such as estradiol and progesterone, and a new intervention strategy is provided for improving follicular development and reproductive performance of sows.

The invention discloses a method for detecting various pollutants in follicular fluid. The method comprises the steps of enzymolysis, extraction, nitrogen blowing, redissolution and detection on a machine. Mixing the follicular fluid sample with the mixed internal standard solution, performing enzymolysis overnight, and hydrolyzing the bound metabolite by using beta-glucuronidase; extracting by using an ethyl acetate / methyl tert-butyl ether mixed solution containing 0.1% formic acid, and repeating for three times to extract 13 types of 182 pollutants; and carrying out nitrogen blowing drying and acetonitrile / water redissolution, and analyzing through a series quadrupole rod liquid chromatograph-mass spectrometer. According to the method, the detection limit is as low as nanogram / milliliter, quantification is completed within a short time through single sample injection, more than 100 samples can be detected in a single day, high-throughput and high-sensitivity detection is achieved, energy consumption and cost are reduced through program optimization, high efficiency and environmental friendliness are achieved, and a powerful means is provided for studying the follicular fluid pollutant exposure level and correlating with reproductive health.

The invention discloses an application of Reco18 in preparation of a product for improving skin glossiness, smoothness, irregular menstruation, sleep or ovarian aging. Reco18 is a nutritional supplement preparation, the core component Reco18 is a component of follicular fluid, the research shows that the Reco18 can improve the skin condition of women and delay ovarian aging, specifically, the Reco18 can remarkably improve the skin glossiness, roughness and smoothness of women, effectively relieve sleep disorder, relieve dysmenorrhea and delay ovarian aging, and the Reco18 can be used for preparing the follicular fluid. The menstrual color is ruddy, and the safety is good.

Provided is a biomarker for diagnosing or predicting reactivity of an ovary to FSH and use thereof. According to a composition or a kit for diagnosing or predicting reactivity of an ovary to FSH and a method using the same according to one aspect, the degree of reactivity of the ovary may be easily diagnosed using miRNA present in blood, follicular fluid, granulosa cells, and cumulus cells, and thus it is easy to predict or diagnose symptoms or diseases caused by abnormal reactivity of the ovary to FSH.

The invention provides application of PON3 protein in preparation of a medicine for preventing and treating polycystic ovarian syndrome, and belongs to the technical field of biological medicine. It is found that the secretion amount of PON3 in follicular fluid of an obese PCOS patient is remarkably reduced, and it is found through further research that the expression level of endoplasmic reticulum stress-related protein in PCOS granular cells under the high fat condition is remarkably increased, and the PON3 protein level is remarkably reduced. The PON3 can be used for inhibiting the occurrence of endoplasmic reticulum stress of PCOS granular cells under a high fat condition through GRP78 / IRE1alpha and GRP78 / PERK pathways. The invention provides a new potential target for the occurrence and development of PCOS diseases.

The invention belongs to the technical field of biology, and particularly relates to application of phosphatidyl glycerol. According to the invention, phosphatidyl glycerol PG (20: 422: 6) is taken as a biomarker, follicular fluid metabolites are analyzed, and high-sensitivity, high-specificity and high-accuracy diagnosis of the hypoovarian reserve function (DOR) is realized.

The application belongs to the technical field of biology and specifically relates to application of phosphatidylethanolamine. The application takes phosphatidylethanolamine PE (16:0_22:5) as a biomarker, realizes high sensitivity, high specificity and high accuracy in diagnosis of decreased ovarian reserve (DOR) by analyzing metabolites of follicular fluid.

The application relates to the technical field of medical devices, and particularly discloses a workstation for assisting in picking up eggs, which is used for constant-temperature conveying of a collection tube containing follicular fluid, and comprises a workstation, a first executing mechanism for opening a cover of the collection tube, and a second executing mechanism for clamping a tube body of the collection tube; the workstation is provided with a delivery window, and a collection tube conveying mechanism for conveying the collection tube is arranged in the delivery window; and a temperature maintaining system is further arranged on the workstation to maintain the temperature of the follicular fluid in the collection tube. The collection tube conveying mechanism arranged in the delivery window is used for conveying the collection tube, and the first executing mechanism and the second executing mechanism are used for realizing the grasping, opening, pouring and discarding of the collection tube, so that special collection tube delivery personnel are not needed, labor is saved, work efficiency is improved, the in-vitro exposure time of the ovum is shortened, and the development potential of the ovum is protected.

The invention discloses a TUDCA-added porcine oocyte culture solution as well as a preparation method and application of the TUDCA-added porcine oocyte culture solution. The TUDCA-added pig oocyte culture solution comprises TUDCA and a basic culture solution, wherein the basic culture solution comprises a TCM-199 culture medium containing a 1% Pen Strep solution, an epidermal growth factor, insulin, pyruvic acid, cysteine, pig follicular fluid, PMSG and hCG; the addition amount of the TUDCA is 0.5 mmol / L of the basic culture solution. The TUDCA-added pig oocyte culture solution can effectively repair heat stress-induced pig oocyte damage, significantly improve the in-vitro mature ovum rate of heat stress-induced pig oocytes, and improve the oocyte quality.

Provided is technology for testing the likelihood of pregnancy and / or the likelihood of childbirth resulting from the pregnancy. Provided is a method of testing the likelihood of pregnancy from an egg that had been contained in a follicular fluid collected from a subject and / or the likelihood of childbirth resulting from the pregnancy, including step (1) of detecting at least one miRNA selected from (a) the group consisting of miR-16-2-3p, miR-378a-3p, miR-483-5p, miR-1246, and miR-1290, and / or from (b) the group consisting of miR-204-3p, miR-1468-5p, miR-424-5p, miR-30e-5p, miR-146a-5p, miR-202-5p, miR-370-3p, miR-506-3p, miR-3184-3p, miR-423-5p, miR-365a-5p, miR-514a-3p, miR-92b-5p, miR-27a-5p, miR-6873-3p, miR-451a, miR-122-5p, and miR-122b-3p, in a sample of the follicular fluid.

The invention relates to the technical field of chemical detection electrodes and medical detection, and particularly discloses a detection electrode, a preparation method of the detection electrode and application of the detection electrode in detection of perfluorinated compounds in follicular fluid. The preparation method of the detection electrode comprises the following steps: coating the surface of a glassy carbon electrode with an electrode material, and drying to obtain the detection electrode, the preparation method of the electrode material comprises the following steps: S1, uniformly mixing plant straw powder with urea to obtain a mixed raw material; and S2, carrying out heating treatment on the mixed raw material, and taking a solid product after the heating treatment is finished, so as to obtain the electrode material. According to the method, the surface of a glassy carbon electrode is coated with the electrode material prepared through the method, and the electrode material has good electrochemical response performance on perfluorohexane sulfonic acid and has important application value.

The invention discloses a human oocyte in-vitro maturation culture solution as well as a preparation method and application thereof, and belongs to the technical field of cell in-vitro culture. The technical problems to be solved are low embryo formation rate and fertilization rate and high early apoptosis rate of immature human oocytes subjected to in-vitro maturation culture. According to the technical scheme, the human oocyte in-vitro maturation culture solution is provided and comprises inorganic salt, energy substances, hyaluronic acid, follicular fluid exosomes, antioxidants, amino acids, proteins, hormones, growth factors and antibiotics.

The present subject matter discloses a device and system of aspirating follicular fluid. In an example, an ovum pick up (OPU) device for aspirating follicular fluid includes a needle having a first end and a second end, opposite to the first end. The first end of the needle is to pierce through an ovarian follicle to aspirate the follicular fluid from the ovarian follicle by a suction force. The OPU device includes a connector having a channel to receive the second end of the needle. The OPU device further includes an elastic tube connected to the connector. The second end of the needle passes through the channel and inside the elastic tube. The elastic tube is compressible, while locating the ovarian follicle and piercing the needle through the ovarian follicle, to regulate the suction force during aspiration of the follicular fluid between two follicles.

The application discloses a wearable ovulation monitoring device and method, and relates to the technical field of monitoring equipment. The device comprises a wearable main body and an ultrasonic probe. The device uses the ultrasonic probe to obtain ultrasonic images of the ovary and multiple follicles in the ovary of a measured object at different monitoring periods. An ultrasonic image processing unit extracts ovulation parameters, including the ovary volume, follicle volume, follicle liquid content and follicle wall thickness, from the ultrasonic images. An ovulation prediction unit determines the growth speed and growth stage according to the ovulation parameters, and generates and outputs ovulation monitoring results using corresponding development evaluation models and ovulation prediction models. The application uses a portable elastic bellyband as the main carrier for follicle monitoring. The ultrasonic probe is installed on the bellyband, which is used on the abdomen. The device has the characteristics of small size, portability, simple operation and easy mastery. The processor can realize accurate estimation of the ovulation period and provide guidance for planned pregnancy or contraception.

The application discloses application of follicular fluid exosome lncLIPE-AS1 in evaluation of embryo quality of patients with polycystic ovary syndrome and belongs to the field of assisted reproductive technology. The application finds that lncLIPE-AS1 is highly expressed in follicular fluid of patients with polycystic ovary syndrome, and finds that lncLIPE-AS1 is correlated with embryo quality and steroid hormone secretion of patients with polycystic ovary syndrome. Therefore, lncLIPE-AS1 can be used as a biomarker for evaluation of embryo development quality in the process of assisted reproduction of patients with polycystic ovary syndrome and prediction of success rate of assisted reproduction, and can also be used for screening of high-quality oocytes of patients with polycystic ovary syndrome and regulation of steroid hormone expression of patients with polycystic ovary syndrome.

The utility model relates to the technical field of follicular fluid collection, in particular to an anti-overflow follicular fluid collection tube frame which comprises a main frame body, a groove is formed in the center of the top of the main frame body, a detection conversion set is arranged on the outer side, close to the top of the main frame body, of the groove, and the detection conversion set comprises a laser set for detecting the liquid level position of follicular fluid. And a feedback group for feeding back according to detection information of the laser group is arranged on one side of the laser group. When the follicular fluid collecting device is used, the laser group is arranged on the outer side, close to the top of the main frame body, of the groove, and when the liquid level position of the follicular fluid is higher than the laser group, the laser intensity, the propagation direction, the wavelength and the like are changed by utilizing the change of a laser transmission medium, so that the liquid level position of the follicular fluid in the collecting pipe body is detected; the feedback set is arranged on one side of the laser set, when it is detected that the liquid level position of follicular fluid is higher than the laser set, the feedback set is used for converting an optical signal into an electric signal and then converting the electric signal into a sound signal, and medical workers are reminded.

The invention provides a method for separating and culturing ovarian granular cells, and belongs to the technical field of cell biology. The method for separating and culturing the ovarian granular cells comprises the following steps: selecting follicles of 3-8mm in ovarian tissues; collecting follicular fluid by a suction method, and filtering impurities; collecting filtrate, centrifuging and taking precipitate to obtain ovarian granular cells; and resuspending the ovarian granulosa cells by using a DMEM / F12 complete medium, culturing the cell suspension, and subculturing when the density of the ovarian granulosa cells reaches 80-90%. The method for separating and culturing the ovarian granulosa cells is high in operability and simple, the extracted ovarian granulosa cells are few in parenchyma cells, the growth state is good after passage, the physiological state is stable, and the passage frequency is increased.

The invention relates to the technical field of intestinal flora regulation and control, and discloses a composition for improving intestinal flora regulation and control of PCOS patients and a preparation method of the composition so as to solve the problem that the intestinal flora of the existing PCOS patients is unbalanced. The method comprises the following steps: firstly, bringing into PCOS women and healthy control which are 18-35 years old and accord with a decudan consensus standard, collecting morning excrement samples, carrying out 16S rRNA gene sequencing, analyzing the diversity and structure of intestinal flora, and identifying differential flora; the method comprises the following steps: detecting blood plasma and follicular fluid metabolites by synchronously adopting a metabonomics technology, analyzing key proteins of follicular fluid in combination with proteomics, constructing a regulatory network through integration of multi-omics data, and establishing a diagnosis and prognosis evaluation model; an individualized intervention scheme is designed, the individualized intervention scheme comprises high-performance inulin, berberine hydrochloride and sodium butyrate particle components, a curative effect monitoring node dynamic adjustment scheme is set, it is expected that after 12 weeks of intervention, core indexes such as insulin resistance and free testosterone of a subject larger than or equal to 80% are remarkably improved, the menstrual cycle recovers rules, and a clinical basis is provided for multi-target accurate treatment of PCOS.

The invention discloses a construction method of a pig follicular granule cell ferroptosis model, and relates to the technical field of biology. The construction method comprises the following steps: rinsing a pig ovary with washing liquor until the washing liquor is clear, soaking, putting into the washing liquor for later use, extracting follicular fluid containing granular cells from the pig ovary, and putting into 10% fetal calf serum; filtering, collecting and centrifuging granular cells, removing supernate, re-suspending the cells, repeating centrifugal cleaning, re-suspending the granular cells in 10% fetal calf serum, adjusting the density of the granular cells, and culturing; and treating the granular cells with an ammonium ferric citrate solution, and cleaning to obtain the pig follicle granular cell ferroptosis model. According to the method provided by the invention, the pig follicular granule cell ferroptosis model can be successfully constructed, the standardization degree and repeatability of the model are remarkably improved, and the problems of unstable induction efficiency and limited application range of a traditional method are effectively solved.

The present invention discloses an in vitro maturation medium for buffalo oocytes containing celastrol, and a culture method using the in vitro maturation medium for buffalo oocytes. The in vitro maturation medium for buffalo oocytes comprises celastrol and a solvent, and the solvent is TCM-199 culture solution containing NaHCO3, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, buffalo estrus bovine serum, yellow cattle follicular fluid, follicle stimulating hormone, luteinizing hormone, and cysteine. The in vitro maturation medium for buffalo oocytes of the present invention can significantly improve the quality of buffalo in vitro matured oocytes and the developmental ability of subsequent embryos. The cost of celastrol is moderate and it is easy to obtain, and it has extremely high popularization value and application prospect in the in vitro production of buffalo embryos.

The embodiment of the utility model discloses a filtering device for filtering follicular fluid. The filtering device comprises a shell with an inner cavity, the shell comprises a first side part, and the first side part is open; the top wall of the shell is provided with at least one first placing hole penetrating from the top surface to the bottom surface of the shell; the first partition plate and the second partition plate are inserted into the inner cavity of the shell from the opening of the first side part; the first partition plate comprises at least one second placing hole which penetrates from the top surface to the bottom surface of the first partition plate and corresponds to the first placing hole; the second partition plate comprises at least one third containing hole which penetrates from the top face to the bottom face of the second partition plate and corresponds to the second containing hole. The collecting pipe is positioned in the second placing hole and the third placing hole; the bottom of the collecting pipe is located below the second partition plate, and the top is located between the first partition plate and the top wall of the shell; the filter is matched with the collecting pipe for use and is positioned in the first placing hole; the bottom of the filter is located between the first partition plate and the top wall of the shell, and the top is located on the top face of the top wall of the shell. A gap is formed between the filter and the collecting pipe.

The utility model discloses a special egg picking vessel for an embryo culture room, which comprises a vessel body, and one side of the bottom end in the vessel body is fixedly connected with a material placing ring. According to the utility model, the black marking convex strip is used as an initial position under a microscope, an ovum is found, after the ovum is found, the pasteur suction pipe is used for transferring the ovum into the material placing ring, and the next ovum is continuously found until the ovum returns to the initial position, so that the whole dish of follicular fluid is completely found, and the follicular fluid can enter and exit from the material placing ring through the open groove; the ovum is left in the material placing ring, the ovum picked in the process is always located in the follicular fluid, the dish body is placed on a heating workbench, the temperature of the ovum can be kept unchanged, after the follicular fluid in the whole dish is found out, all the ovum is sucked out of the material placing ring, the follicular fluid is poured out from the position marked by the black transverse strip, and even if the follicular fluid is left on the outer wall, the ovum can be placed in the material placing ring. The fingers are always located at the limiting blocks on the two sides and are completely separated from the limiting blocks, and the pollution risk caused by the fact that the fingers are contaminated by follicular fluid is effectively avoided.

The invention provides application of Jinfeng pills in preparation of a medicine for treating ovarian hyporesponsiveness. The Jinfeng pill provided by the invention can significantly improve the E2 and TT levels in serum and follicular fluid of a POR patient, and improve the mature ovum rate, the high-quality embryo rate, the clinical pregnancy rate and the living yield of the POR patient, thereby improving the IVF-ET treatment outcome of the POR patient.