93 results about "Follicular fluid" patented technology

A device and method for assessing characteristics of follicular fluid in vivo comprises an aspiration needle for oocyte retrieval having one or more sensors disposed on its outer cannula wall or embedded in its outer cannula wall for sensing and measuring characteristics of follicular fluid constituents such as pH level and oxygen concentration. The sensors are microsensors that communicate through a physical connection or wirelessly with a meter and display. The measurements are recorded and optionally printed for later review and selection of the most viable oocytes. The method involves inserting into an ovary an aspiration needle with one or more sensors. The needle is guided to a first follicle and inserted in the first follicle. Characteristics of the follicular fluid are sensed and measured and accordingly displayed on the meter and recorded for later reference and selection. The fluid is collected into a first collection container. The sensors can then be reset to a reference baseline and then additional follicles can be aspirated and measured in the same manner. After collection, the measurements of follicular fluid characteristics for each follicle can be assessed and the most viable oocytes can be selected.

The present invention discloses a method for researching the role of melatonin in predicting the outcome of ovarian reserve and in vitro fertilization-embryo transfer (IVF-ET), including: collecting basic clinical data of 61 women, and according to their ovary response to stimulation, mainly by age , the number of retrieved eggs, and the value of anti-Müllerian hormone were divided into three groups: ovarian low response group, normal group and high response group. The follicular fluid of 61 women was collected, the melatonin concentration in the follicular fluid of 61 women was measured by melatonin radioimmunoassay, and the melatonin concentration and other clinical data were analyzed in the ovarian low response group, normal group and high response group. level; analyze the relevant data of in vitro fertilization outcome in ovarian low response group, normal group, and high response group, and verify the correlation between melatonin concentration and in vitro fertilization-embryo transfer outcome; verify the relationship between melatonin level and ovarian Relevance of reserves. The invention can predict the outcome of ovarian reserve and in vitro fertilization-embryo transfer by detecting the melatonin concentration in the follicular fluid.

The invention relates to a method for in-vitro fertilization and embryo culture by collecting bovine oocytes in vivo. Specifically, on one hand, the bovine in-vitro fertilization embryo culture methodcomprises the following steps of: taking follicular fluid derived from ovum pick-up of bovine living body, picking out a cumulus-oocyte complex wrapped with at least containing three layers of cumulus cells under a stereoscopic microscope, putting the cumulus-oocyte complex into a transport culture solution, and transporting the cumulus-oocyte complex back to a laboratory within 24 hours; washingCOCs once in an oocyte maturation culture solution and then transferring into a new maturation culture solution for culturing for 22-24 h under the culture conditions that the temperature is 38.8 DEGC, the concentration of CO2 is 5.5-6.5% and the humidity is saturated; performing in vitro fertilization; and carrying out embryo in vitro culture and preservation. The invention also relates to a transport culture solution for oocytes. The method and the transportation culture solution disclosed by the invention show excellent technical effects as shown in the specification.

The invention provides a method for enhancing the ectogenesis of sheep oocyte. The method adopts an adenylate cyclase activator Forskolin (FSK), phosphodiesterase inhibitor cilostamide (CIL) and 3-isobutyl-1-methylxanthine (IBMX), treats the sheep oocyte in a combining manner and improves the capability of the ectogenesis of the sheep oocyte. The method comprises the following detailed steps of: picking the ovary of the killed sheep, putting the ovary of the killed sheep into normal saline for cleaning for 3-4 times; pumping the 2-8mm follicles on the surface of the ovary by using a 10ml syringe which absorbs 1ml ovum-pumping liquid containing 100mu mol / L FSK and 500mumol / L IBMX and is provided with a number-9 needle head, and injecting the follicular fluid recovered in the syringe into a35-60mm vessel; picking the oocyte under a microscope, putting the oocyte into the pumped follicular fluid, cleaning the oocyte for three times with in-vitro mature fluid containing 20mu mol / L CIL, and putting the oocyte in a CO2 incubator for culture; then cleaning the oocyte for three times with in-vitro mature fluid which does not contain CIL, putting the oocyte in the CO2 incubator for culture and adding into in-vitro fertilization fluid drop; unfreezing the seminal fluid with water bathing, and commonly incubating with the oocyte; and calculating the cleavage rate after 24 hours and calculating the blastocyst rate after 168 hours.

Disclosed is a method for cloning buffalo's body cells comprising the following steps: charging calf follicular fluid and epithelium cell growth factor into maturation to cultivate acceptor oocyte, enucleating under spindle image system, cultivating and processing donor cells with DNA synthesized depressant Aphidicolin, carrying out hungry cultivation, activating the recombinant embryo with ionomycin and 6-dimethoxy aminopurine, and proceeding embryo extracorporal cultivation with modified TCM 199 and estrus cow's serum.

The invention relates to an oocyte quality evaluation method. The method comprises the step of comparing the composition of follicular fluid metabolites of a subject with low ovarian reserve functionwith that of a healthy subject. The characteristic biomarker is screened through metabonomics analysis, the relationship between the follicular fluid metabolism marker group and the quality of the oocytes is determined, and a more objective and noninvasive oocyte quality evaluation method is established.

The invention provides a method for judging a cell state in a body liquid environment based on a liquid-phase chip technology. A biological marker of a follicular fluid is analyzed by using the liquid-phase chip technology, so as to judge the quality of forming an embryo by an ovum from the same follicular of the follicular fluid; good-quality embryo selection is determined by combining with a morphological analysis. With the adoption of the method provided by the invention, the clinical pregnancy rate and the embryo implantation rate of IVF (In-Vitro Fertilization) can be improved by providing an objective indicator of the ovum quality, so as to improve the result of IVF therapy. A plurality of factor combinations for judging the ovum quality of the follicular fluid can be produced into a commercial liquid-phase chip kit which is used for assisting the reproduction technology.

The present invention relates to a biomarker for diagnosing or predicting reactivity of an ovary to FSH and a use thereof. According to a composition for diagnosing or predicting reactivity of an ovary to FSH, a kit, and a method using same in accordance with one aspect, the degree of reactivity of the ovary can be easily diagnosed using miRNA present in blood, follicular fluid, granule cells, and cumulus cells, and thus, it is easy to predict or diagnose symptoms or diseases caused by abnormal reactivity of an ovary to FSH.

The invention discloses a collecting device for follicular fluid of an animal in vitro. The collecting device comprises a collecting device body, a flexible pipe, a metal pipe, a buffer solution pipe,a thin plug, an air suction pipe, a rubber plug and a collecting bottle, the collecting device body is composed of a handle, a concave cavity, a rubber ring, an upper cover and several puncturing needles, the upper cover is connected with the flexible pipe, and the flexible pipe is connected with the metal pipe; the buffer solution pipe is composed of an injector adapter located at the upper endof the buffer solution pipe, a middle pipe body and a flushing ball located at the lower end of the buffer solution pipe, and several small holes are formed in the flushing ball; the bottle opening ofthe collecting bottle is plugged by the rubber plug, and the ends of the metal pipe, buffer solution pipe and air suction pipe are all inserted in the rubber plug and appear above the collecting bottle; the other end of the air suction pipe is connected with a vacuum pump, the lower end of the collecting bottle is provided with a glass valve, and a liquid outlet hole is formed in the glass valve.The collecting device for the follicular fluid of the animal in vitro can simply conveniently collect the follicular fluid of the animal in vitro, contamination on a sample cannot be caused during the operative process, and a pipeline can be cleaned conveniently.