38 results about "Granular cell" patented technology

The invention discloses application of a Wnt2 gene in buffalo ovarian granular cells, and belongs to the technical field of cell engineering and gene engineering. Wnt2 is taken as a research object, the Wnt2 overexpression vector or Wnt2 siRNA is transfected to the ovarian granulosa cells respectively by constructing the Wnt2 overexpression vector and synthesizing small interfering RNA (Wnt2 siRNA), then changes of signal channel gene mRNA and protein level related to ovarian granulosa cell apoptosis, proliferation and E2 and P4 secretion are detected, and finally phenotypic changes of the ovarian granulosa cells are detected. Results show that Wnt2 can inhibit apoptosis of ovarian granular cells and promote proliferation and E2 and P4 secretion. By exploring the application of the Wnt2 in the ovarian granular cells, the application has good application in researching an ovarian follicle atresia mechanism, initial estrus initiation and the like.

The invention provides an establishment method of umbilical cord mesenchymal stem cells with high expression of IGF-1 (Insulin-like Growth Factor 1) and application of the umbilical cord mesenchymal stem cells in premature ovarian failure. The construction method of the umbilical cord mesenchymal stem cells with high expression of IGF-1 comprises the step of transfecting the umbilical cord mesenchymal stem cells with the IGF-1 gene, so that the umbilical cord mesenchymal stem cells highly express IGF-1. The invention also provides application of the umbilical cord mesenchymal stem cell with high expression of IGF-1 in preparation of drugs for treating premature ovarian failure. According to the invention, the IGF-1 gene is transfected to the hUC-MSC, so that the hUC-MSC highly expresses the IGF-1, and the hUC-MSC secretes a large amount of IGF-1. The ovarian function is improved through the immunoregulation effect of hUC-MS, and meanwhile, the effect of resisting apoptosis of granular cells is achieved through IGF-1 secreted by hUC-MS. And the treatment effect on the premature ovarian failure is achieved through synergism of the two effects.

The invention relates to the technical field of biological breeding, in particular to application of an RBX1 gene in cow ovarian granular cells. According to the invention, the RBX1 gene is specifically regulated on the ovarian granulosa cells, and the RBX1 gene is found to have the effect of promoting the proliferation of the granulosa cells; and the gene is provided for the creation of a new high-fecundity breeding material for gene editing cattle in the later period.

The invention discloses application of an SFRP1 gene in buffalo ovarian granular cells, and belongs to the technical field of cell engineering and gene engineering. According to the invention, SFRP1 is taken as a research object, an SFRP1 overexpression vector or siRNA-SFRP1 is respectively transfected to ovarian granular cells by constructing the SFRP1 overexpression vector and synthesizing small interfering RNA (siRNA-SFRP1), then changes of signal channel gene mRNA and protein level related to ovarian granular cell apoptosis, proliferation and E2 and P4 secretion are detected, and finally phenotypic changes of the ovarian granular cells are detected. Results show that the SFRP1 can inhibit apoptosis of ovarian granular cells and promote proliferation and E2 and P4 secretion. The application of the SFRP1 in the ovarian granular cells is explored, and the SFRP1 has good application in research of an ovarian follicle atresia mechanism, initial estrus initiation and the like.

The invention provides an ovary-targeting ROS response type SS31 nano-particle and a preparation method and application thereof, and belongs to the field of biomedicine.The ovary-targeting ROS response type SS31 nano-particle is constructed by utilizing a porous metal organic framework to carry hyaluronic acid and utilizing an ROS response type thioketone linker to be combined with mitochondrial targeting peptide SS31, and the ovary-targeting ROS response type SS31 nano-particle is used for preparing the ovary-targeting ROS response type SS31 nano-particle. And the SS31 is targeted and delivered to the ovarian tissue. The nanoparticles target ovary and slowly release SS31 under the action of ROS, so that oxidative stress of oocytes and granular cells caused by female reproductive system diseases such as premature ovarian failure, polycystic ovarian syndrome and the like is reduced, and the mitochondrial function of the oocytes and the granular cells is improved; and a foundation is laid for the subsequent clinical treatment of diseases related to the mitochondrial dysfunction of the female reproductive system by using the SS31.

The invention relates to the technical field of ovarian granule cell apoptosis, and discloses a method for regulating oxidative stress induced sheep ovarian granule cell apoptosis by using allicin, and the method comprises the following steps: firstly preparing a culture solution, then preparing an allicin stock solution, and identifying sheep ovarian granule cells; the method comprises the following steps: firstly, measuring the activity of ovarian granular cells after independent treatment of H2O2 and allicin, determining the optimal treatment time and concentration of H2O2 and allicin, then carrying out co-treatment of H2O2 and allicin, detecting the apoptosis of the granular cells by using flow cytometry, detecting the expression of apoptosis-related genes by using real-time fluorescent quantitative PCR, detecting the secretion of steroid hormones by using ELISA, and carrying out data analysis. The invention solves the problems that in the prior art, an effective regulation and control method for ovarian granular cell apoptosis induced by oxidative stress is lacked, and traditional medicines possibly have large side effects, unobvious effects and the like.

The invention provides an application of a CTH inhibitor and / or an xCT inhibitor in preparation of a medicine for inhibiting mammal embryo implantation, and relates to the technical field of medicines. The application effect of a cystathionine-gamma-lyase (CTH) inhibitor and / or a solute carrier family 7 member 11 (xCT) inhibitor in inhibition of mammalian embryo implantation is verified through research. The research result shows that the xCT inhibitor and / or the CTH inhibitor can obviously inhibit proliferation of corpus luteinized granular cells and secretion of progesterone of a model mouse; meanwhile, by injecting the xCT inhibitor and / or the CTH inhibitor into the mouse model in vivo, ovary ovulation and the maturation rate of the ovulated oocyte nucleus can be remarkably inhibited, functional corpus luteum formation and embryo implantation can be remarkably reduced, and then a new thought and method are provided for development of contraceptive drugs.

The invention discloses a preparation method of mesenchymal stem cells for resisting ovarian granular cell aging. The preparation method comprises the following steps: providing umbilical cord blood stem cells; carrying out induced culture on the umbilical cord blood stem cells, collecting a supernatant after the culture is completed, and separating the supernatant to obtain an exosome; providing mesenchymal stem cells, and performing induced culture on the mesenchymal stem cells by adding the exosome, so as to obtain the required mesenchymal stem cells for resisting ovarian granular cell senescence. According to the preparation method of the mesenchymal stem cells for resisting ovarian granular cell senescence, the mesenchymal stem cells induced by the umbilical cord blood stem cell exosome can be prepared, and the mesenchymal stem cells can be used for treating premature ovarian failure in a whole body vein use mode. The umbilical cord mesenchymal stem cells induced by the stem cell exosome can exert a better immune regulation function, regulate the balance of an immune system and reduce immune-related inflammation and injury so as to delay the senescence of ovarian granular cells.

The invention discloses an antioxidant nano material as well as a preparation method and application thereof, and belongs to the technical field of material preparation. On the basis of the reduction reaction of GSH and selenium-containing substances, oxidation of selenium is effectively protected by utilizing a protective atmosphere, and it is ensured that the selenium element is combined with GSH in a stable valence state. Finally, a novel selenium nano material (GSH-Se) is synthesized, and the GSH-Se has antioxidant ability and GSH-Px-like activity, can relieve oxidative stress induced primary yak follicular granular cell and mouse ovary steroid hormone synthesis disorder, cell apoptosis and mitochondrial division fusion abnormality, and can be used for preparing a novel selenium nano material (GSH-Se). Meanwhile, the GSH-Se can regulate and control steroid hormone synthesis and cell apoptosis of the yak follicular granular cells by inducing mitochondrial fusion, and a new thought is provided for application of the GSH-Se to improvement of female reproductive performance.

The invention provides a novel application of a raspberry extract. It is found through tests that any one of isoquercitrin, ellagic acid, vanillic acid, oleanolic acid, kaempferol-3-O-rutinoside, kaempferol-3-O-sophoroside, tiliroside, tormentic acid and raspberry extract can remarkably improve the activity of the human ovarian granular cells or remarkably improve the oxidative stress level, and the activity of the human ovarian granular cells can be remarkably improved. According to the present invention, the ovarian function can be effectively protected by the eight components, the ovarian function can be effectively protected by the eight components, or the mitochondrial membrane potential level can be significantly regulated and controlled, or the estrogen level in the microenvironment can be effectively maintained, such that the ovarian function can be effectively protected by the eight components, and the raspberry extract containing the eight components. The invention provides a new choice for ovarian function protection products, and also provides a new application for the substances.

The invention relates to an isolated culture method of functional Chinese softshell turtle ovarian granular cells. The isolated culture method comprises the following steps: S1, collecting ovarian tissues: collecting Chinese softshell turtle ovarian tissues; s2, separating and collecting a granular cell layer; s3, digesting and collecting granular cells; s4, primary culture of granulosa cells: transferring the collected cells into a cell culture bottle containing 4 ml of a complete culture medium for primary culture, wherein the complete culture medium comprises the following main components: a DMEM culture medium, 15% of fetal calf serum, 1% of penicillin streptomycin and 1% of an insulin-transferrin-selenium additive; and S5, subculturing the granular cells. The isolated culture method and the culture medium system provided by the invention are simpler and more time-saving and labor-saving; according to the method, the aromatase CYP19A1 can be expressed normally, the characteristics of the granular cells are kept, the aromatase CYP19A1 can be expressed normally, it is indicated that the in-vitro cultured cells can keep the functionality of the cells, and the in-vitro proliferation capacity of the granular cells is improved to a great extent.

The invention belongs to the field of traditional Chinese medicine, and particularly relates to Kunxinning and a preparation method and application thereof. The effective components of the Kunxingning disclosed by the invention are artemisinin and artemisinin. Animal experiments prove that kunxingning can improve the early-onset ovarian insufficiency toxicity state of SD rats caused by tripterygium glycosides, reduce ovarian granular cell apoptosis, improve the ovarian pathological state, promote follicular development, restore the serum sex hormone level to be normal to a certain extent, improve the state of proportion imbalance of Th17 / Treg immune cells, and improve the early-onset ovarian insufficiency toxicity of the SD rats. And the mood cycle of the rats is recovered to a certain extent. Meanwhile, the physiological activity of the tripterygium glycosides composition obtained by compounding the kunxingning and the TG is equivalent to that of the TG.

The invention discloses an ergothioneine composition for improving ovarian vitality and a preparation method and application thereof, the composition comprises D-ribose and ergothioneine, the concentration of the D-ribose in the composition is 5-15 mM, and the concentration of the ergothioneine in the composition is 100-300 [mu] M. The pharmaceutical composition is used for regulating the redox balance of ovarian granular cells, inhibiting inflammatory response and up-regulating anti-mullerian hormone secretion so as to improve the ovarian activity.

The invention provides an application of an E3 ubiquitin ligase RLIM gene inhibitor in preparation of a medicine for treating ovarian aging or injury. Experiments find that the expression level of the RLIM gene in the ovary is positively correlated with the aging degree, and the expression of the RLIM gene is remarkably improved along with age increase. In-vivo and in-vitro experiments show that ovarian granular cell senescence can be effectively delayed and ovarian functions can be improved by inhibiting RLIM expression, and particularly, the proportion of atresia follicles is reduced, the number of healthy follicles is increased, and serum AMH and E2 levels are remarkably improved. The pharmaceutical composition provided by the invention comprises a siRNA inhibitor (preferably, siRLIM-2) of specific targeting RLIM and a nano-liposome carrier, and efficient targeting delivery can be realized through tail vein injection. The treatment scheme has no toxic effect on important organs, is high in safety, and provides a new effective means for treatment of ovarian aging and related diseases.

The invention provides a method for regulating and controlling the transcriptional activity of a PAFAH1B1 gene and application of the method, and belongs to the technical field of gene expression regulation and control. The method comprises the step of influencing the methylation level of a PAFAH1B1 gene promoter region by using a gRNA fragment, a DNA methyltransferase inhibitor or an RNA interference fragment of DNA methyltransferase. The method is applied to the ovarian granulosa cells, and under different treatments, the mRNA level and the protein expression level of PAFAH1B1 in the ovarian granulosa cells can be remarkably promoted or inhibited. According to the application, the obvious influence of DNA methylation on the transcriptional activity of the PAFAH1B1 gene in the ovarian granular cells is determined for the first time, the position of the DNA methylation applied to the promoter region of the PAFAH1B1 gene is determined for the first time, and the mechanism that the DNA methylation affects the expression of the PAFAH1B1 gene by changing the chromatin structure of the promoter region of the PAFAH1B1 gene is disclosed, so that the expression of the PAFAH1B1 gene in the ovarian granular cells is improved. The invention provides a simpler and more effective new way for regulating and controlling granular cell functions and ovarian development, and has important application value for subsequent research on mammal ovarian granular cell functions, follicular development and initial estrus initiation.

The invention relates to an application of an HK1 gene in mouse ovarian granular cells, in particular to an application in regulating proliferation and apoptosis of the mouse ovarian granular cells and / or steroid hormone synthesis. According to the invention, mouse ovarian granular cells are taken as a research object, and the expression of the HK1 gene is interfered to verify that the HK1 gene is taken as a key target spot for regulating and controlling the functions of the mouse ovarian granular cells so as to influence steroid hormone synthesis and cell proliferation and apoptosis processes. The invention not only provides more reliable technical support for ovarian function regulation, but also provides new thinking and technical guidance for mechanism research of reproductive diseases and preparation and development of related drugs.

The invention provides application of PON3 protein in preparation of a medicine for preventing and treating polycystic ovarian syndrome, and belongs to the technical field of biological medicine. It is found that the secretion amount of PON3 in follicular fluid of an obese PCOS patient is remarkably reduced, and it is found through further research that the expression level of endoplasmic reticulum stress-related protein in PCOS granular cells under the high fat condition is remarkably increased, and the PON3 protein level is remarkably reduced. The PON3 can be used for inhibiting the occurrence of endoplasmic reticulum stress of PCOS granular cells under a high fat condition through GRP78 / IRE1alpha and GRP78 / PERK pathways. The invention provides a new potential target for the occurrence and development of PCOS diseases.

The invention discloses a method for regulating and controlling SLIT2 transcription and application of the method in ovarian granular cells, and belongs to the technical field of cell engineering and gene engineering. According to the invention, SLIT2e, SLIT2 gene promoter region histone acetylation and an SLIT2 gene are taken as research objects, and the SLIT2 gene promoter histone acetylation is verified through TSA or HDAC-siRNA to promote the expression of the SLIT2 gene promoter histone acetylation. The invention explores the mechanism that the enhancer regulates the gene promoter region histone acetylation and chromatin opening degree so as to regulate the transcription expression of the SLIT2 gene. The invention has a certain application value for researching the molecular mechanism of influence of histone acetylation of the SLIT2 gene promoter region on ovarian follicle development.

The application discloses a cerebellar dynamic sequence motion continuous learning control network and a method thereof. The control network comprises an input layer, an intermediate layer and an output layer. The intermediate layer is composed of a feedforward inhibition module and a feedback inhibition module. The input layer generates a mossy fiber signal with dynamic timing. The intermediate layer sparsely samples the input mossy fiber signal through granule cells, and the inhibition module dynamically modulates the activity level and encoding mode of the granule cells. The feedforward inhibition module averages the mossy fiber signal discharge rate to obtain the activity attribute of Golgi cells, which is inherited to the granule cells. The feedback inhibition module sparsely samples the granule cells to obtain the activity attribute of Golgi cells, which is inherited to the granule cells. The output layer sequentially outputs the multiplication result of the activity range of the granule cells at each time point and the connection weight between the corresponding Purkinje cells as the control cerebellar signal. The application improves the problem that the biological constraint cerebellar network is not accurate and the motion conversion is not smooth in sequence motion continuous learning.

The invention discloses a continuous learning control network aiming at cerebellum dynamic sequence motion and a method thereof. The control network comprises an input layer, a middle layer and an output layer, the middle layer is composed of a feedforward suppression module and a feedback suppression module; the input layer generates a moss fiber signal with a dynamic time sequence; the middle layer carries out sparse sampling on input moss fiber signals through granular cells, and meanwhile, the inhibition module dynamically modulates the activity level and coding mode of the granular cells; wherein the feed-forward suppression module is used for averaging a moss fiber signal discharge rate to obtain activity attributes of Golgi cells and inheriting the activity attributes to granular cells; the feedback inhibition module carries out sparse sampling treatment on the granular cells to obtain activity attributes of the Golgi cells and inherits the activity attributes to the granular cells; and the output layer sequentially outputs a result of multiplication of a connection weight between the granular cell activity range and the corresponding Purkinje cell in each time point as a cerebellum control signal. According to the invention, the problems of inaccuracy and unsmooth action conversion of a biologically constrained cerebellum network in sequence motion continuous learning are solved.

The invention relates to a strategy determination method and electronic equipment, which are used for improving the strategy determination accuracy. Comprising the following steps: inputting a to-be-processed pulse signal into a decision model; wherein the decision model comprises two first modules and a Purkinje cell layer, and any one of the first modules comprises a moss fiber layer and a granular cell layer; for any first module, inputting a to-be-processed pulse signal to the granular cell layer through the moss fiber layer; performing high-dimensional expansion processing and sparse processing on the pulse signal to be processed by using the granular cell layer to obtain an intermediate pulse signal; the Purkinje cell layer is used for prolonging the time scale of instantaneous input stimulation and gradually accumulating stimulation on intermediate pulse signals obtained by the two first modules respectively, and working memory is realized by self-discharge of two groups of Purkinje neurons in the Purkinje cell layer. And strong mutual inhibition between the two groups of Purkinje neurons is utilized to complete a win-win eating process, and a target decision is obtained.

The invention relates to a composition containing stem cells and application of the composition in ovarian aging resistance. According to the invention, the polypeptide TP-TOF with ovarian aging resistance is separated and screened from placenta, and the polypeptide can effectively reduce apoptosis of ovarian granular cells. The polypeptide can effectively promote recovery of ovarian estradiol and follicular poietin levels of rats independently or in combination with stem cells, can remarkably prolong the estrus period, reduce the estrus interval, reduce follicle apoptosis and promote follicle generation, and has a wider application prospect.

The invention discloses an in-vitro stable subculture construction method of a yak ovarian granular cell line. Relates to the technical field of cell culture. Comprising the following steps: carrying out subculture and amplification on primary yak ovarian granular cells to obtain yGCs; and carrying out pCI-neo-hTERT plasmid transfection and establishment of a monoclonal cell. The immortalized yGCs constructed by using an hTERT plasmid method can provide infinitely proliferated homogenized cells, reduce damage to yaks and avoid individual differences, can retain the core function of regulating follicular development of yGCs, provides a high-quality model for analyzing molecular mechanisms such as seasonal oestrus and low reproduction efficiency of yaks, and has theoretical and application values.

The invention relates to an application of an IDH1 gene in porcine ovarian granular cells, in particular to an application in regulating proliferation and apoptosis of the porcine ovarian granular cells and / or steroid hormone synthesis and / or reproductive performance related gene expression. According to the invention, porcine ovarian granular cells are taken as objects, and the expression of the IDH1 gene is regulated to verify that the IDH1 gene is taken as a key target spot for regulating and controlling the functions of the porcine ovarian granular cells so as to influence steroid hormone synthesis and cell proliferation and apoptosis processes. A new molecular target is provided for improving the reproductive performance of the sow, more reliable technical support is provided for regulating and controlling the ovarian function, and a new thought and technical guidance are provided for mechanism research of reproductive diseases and preparation and development of related drugs.

The invention discloses a GV-stage granulosa cell extraction method, which is characterized in that the number of granulosa cells is diluted to 4-6 * 10 < 5 > / L in the extraction process, the extracted cells can be utilized to the maximum extent, PSB is used for flushing in the extraction process, and high-glucose DMEM containing 0.8-1.2% of double antibodies and 8-12% of FBS is used, so that adherent culture of the granulosa cells is neater and cleaner, and the GV-stage granulosa cells are obtained. And adherence and growth of GV-stage granular cells are facilitated.

The invention discloses application of spermidine in preparation of a preparation for resisting oxidative stress injury of ovarian granular cells, and belongs to the technical field of biological medicines. The content of the invention shows that spermidine can improve the ovarian endocrine function and reproductive capacity of oxidative stress mice, normalize the estrus cycle, relieve oxidative stress, increase ovarian reserve and reduce GC cell apoptosis, and verifies that spermidine indeed has the effect of resisting oxidative stress of ovarian granular cells. A thought is provided for solving the problem of oxidative stress of the ovarian granular cells.

The application provides a cost entropy information granulation semi-supervised feature selection method for diabetes detection, and belongs to the technical field of intelligent medical treatment, and the technical scheme is as follows: S1, a weighted granular cell is constructed; S2, a hemispherical granulation is performed on each decision class based on covariance matrix principal direction analysis, and a pseudo label is assigned to an unmarked sample; S3, a cost entropy is introduced for feature evaluation according to feature difference, and feature importance is obtained; S4, the feature importance is sorted, and a feature subset is selected; and S5, the optimal feature subset is obtained by aggregating the feature selection results of each subnode. The application improves the classification accuracy of a multi-dimensional feature diabetes data set, reduces the calculation complexity of a classification method, improves the accuracy and efficiency of feature selection, helps doctors to judge whether a patient is suffering from diabetes, and has strong application value.

The utility model discloses a cell impurity removal vessel which comprises a culture dish body and a culture dish cover, three partition plates are arranged in the culture dish body and divide the culture dish body into three fan-shaped areas, one fan-shaped area is set as a cleaning area, the interior of the cleaning area is divided into a smooth part and a cleaning part, and the smooth part and the cleaning part are arranged in the culture dish body. And concave-convex lines are arranged on the cleaning part. The cell sap can be stored in a partitioned mode, granular cells can be rapidly scraped off from oocytes through the concave-convex lines in the cleaning area during operation, and the cleaning work efficiency of the oocytes is effectively improved.

The invention discloses a method for improving the reproductive performance of cattle by using betaine, and belongs to the technical field of animal reproduction biology. The method comprises applying an effective dose of betaine to bovine oocytes and / or granular cells thereof; the betaine is a natural quaternary ammonium type alkaloid with an anti-oxidation function. According to the invention, it is found through metabonomics that betaine is a key metabolite of cattle high-yield breeding traits for the first time, and it is proved that betaine has double beneficial effects in a cattle breeding system: on a granular cell level, the level of active oxygen in cells induced by hydrogen peroxide can be significantly reduced by adding 5 mM betaine, and oxidative stress can be effectively relieved; on the oocyte level, 0.5 mM betaine is added into an in-vitro maturation and / or embryo culture solution, so that the cleavage rate and blastocyst formation rate of the bovine in-vitro embryo can be remarkably increased. The invention provides a brand-new, safe and effective solution for improving the production efficiency and the reproductive capacity of the bovine in-vitro embryo.

The invention discloses a miRNA (micro Ribonucleic Acid) marker related to ovarian granular cell proliferation of small tailed han sheep as well as a detection primer and application of the miRNA marker. The marker is novel-m0298-5p, and the sequence of the novel-m0298-5p is as shown in SEQ ID NO. 1. According to the invention, a novel-m0298-5p targeted WNT5A gene is predicted through bioinformatics, and then technologies such as a dual luciferase report system, an overexpression and knock-down test, RT-qPCR, Western blot, CCK-8, EdU and the like are utilized to further verify that the miRNA marker can inhibit the proliferation of ovarian granular cells of small tailed han sheep, so that the miRNA marker can inhibit the proliferation of ovarian granular cells of small tailed han sheep. The provided novel-m0298-5p can be used as a biomarker to be applied to identification of proliferation of ovarian granular cells of the small tailed han sheep.