21 results about "Granular cell" patented technology

The invention provides a novel application of a raspberry extract. It is found through tests that any one of isoquercitrin, ellagic acid, vanillic acid, oleanolic acid, kaempferol-3-O-rutinoside, kaempferol-3-O-sophoroside, tiliroside, tormentic acid and raspberry extract can remarkably improve the activity of the human ovarian granular cells or remarkably improve the oxidative stress level, and the activity of the human ovarian granular cells can be remarkably improved. According to the present invention, the ovarian function can be effectively protected by the eight components, the ovarian function can be effectively protected by the eight components, or the mitochondrial membrane potential level can be significantly regulated and controlled, or the estrogen level in the microenvironment can be effectively maintained, such that the ovarian function can be effectively protected by the eight components, and the raspberry extract containing the eight components. The invention provides a new choice for ovarian function protection products, and also provides a new application for the substances.

The invention relates to an isolated culture method of functional Chinese softshell turtle ovarian granular cells. The isolated culture method comprises the following steps: S1, collecting ovarian tissues: collecting Chinese softshell turtle ovarian tissues; s2, separating and collecting a granular cell layer; s3, digesting and collecting granular cells; s4, primary culture of granulosa cells: transferring the collected cells into a cell culture bottle containing 4 ml of a complete culture medium for primary culture, wherein the complete culture medium comprises the following main components: a DMEM culture medium, 15% of fetal calf serum, 1% of penicillin streptomycin and 1% of an insulin-transferrin-selenium additive; and S5, subculturing the granular cells. The isolated culture method and the culture medium system provided by the invention are simpler and more time-saving and labor-saving; according to the method, the aromatase CYP19A1 can be expressed normally, the characteristics of the granular cells are kept, the aromatase CYP19A1 can be expressed normally, it is indicated that the in-vitro cultured cells can keep the functionality of the cells, and the in-vitro proliferation capacity of the granular cells is improved to a great extent.

The invention discloses an ergothioneine composition for improving ovarian vitality and a preparation method and application thereof, the composition comprises D-ribose and ergothioneine, the concentration of the D-ribose in the composition is 5-15 mM, and the concentration of the ergothioneine in the composition is 100-300 [mu] M. The pharmaceutical composition is used for regulating the redox balance of ovarian granular cells, inhibiting inflammatory response and up-regulating anti-mullerian hormone secretion so as to improve the ovarian activity.

The invention provides an application of an E3 ubiquitin ligase RLIM gene inhibitor in preparation of a medicine for treating ovarian aging or injury. Experiments find that the expression level of the RLIM gene in the ovary is positively correlated with the aging degree, and the expression of the RLIM gene is remarkably improved along with age increase. In-vivo and in-vitro experiments show that ovarian granular cell senescence can be effectively delayed and ovarian functions can be improved by inhibiting RLIM expression, and particularly, the proportion of atresia follicles is reduced, the number of healthy follicles is increased, and serum AMH and E2 levels are remarkably improved. The pharmaceutical composition provided by the invention comprises a siRNA inhibitor (preferably, siRLIM-2) of specific targeting RLIM and a nano-liposome carrier, and efficient targeting delivery can be realized through tail vein injection. The treatment scheme has no toxic effect on important organs, is high in safety, and provides a new effective means for treatment of ovarian aging and related diseases.

The invention relates to an application of an HK1 gene in mouse ovarian granular cells, in particular to an application in regulating proliferation and apoptosis of the mouse ovarian granular cells and / or steroid hormone synthesis. According to the invention, mouse ovarian granular cells are taken as a research object, and the expression of the HK1 gene is interfered to verify that the HK1 gene is taken as a key target spot for regulating and controlling the functions of the mouse ovarian granular cells so as to influence steroid hormone synthesis and cell proliferation and apoptosis processes. The invention not only provides more reliable technical support for ovarian function regulation, but also provides new thinking and technical guidance for mechanism research of reproductive diseases and preparation and development of related drugs.

The invention provides application of PON3 protein in preparation of a medicine for preventing and treating polycystic ovarian syndrome, and belongs to the technical field of biological medicine. It is found that the secretion amount of PON3 in follicular fluid of an obese PCOS patient is remarkably reduced, and it is found through further research that the expression level of endoplasmic reticulum stress-related protein in PCOS granular cells under the high fat condition is remarkably increased, and the PON3 protein level is remarkably reduced. The PON3 can be used for inhibiting the occurrence of endoplasmic reticulum stress of PCOS granular cells under a high fat condition through GRP78 / IRE1alpha and GRP78 / PERK pathways. The invention provides a new potential target for the occurrence and development of PCOS diseases.

The invention discloses a method for regulating and controlling SLIT2 transcription and application of the method in ovarian granular cells, and belongs to the technical field of cell engineering and gene engineering. According to the invention, SLIT2e, SLIT2 gene promoter region histone acetylation and an SLIT2 gene are taken as research objects, and the SLIT2 gene promoter histone acetylation is verified through TSA or HDAC-siRNA to promote the expression of the SLIT2 gene promoter histone acetylation. The invention explores the mechanism that the enhancer regulates the gene promoter region histone acetylation and chromatin opening degree so as to regulate the transcription expression of the SLIT2 gene. The invention has a certain application value for researching the molecular mechanism of influence of histone acetylation of the SLIT2 gene promoter region on ovarian follicle development.

The application discloses a cerebellar dynamic sequence motion continuous learning control network and a method thereof. The control network comprises an input layer, an intermediate layer and an output layer. The intermediate layer is composed of a feedforward inhibition module and a feedback inhibition module. The input layer generates a mossy fiber signal with dynamic timing. The intermediate layer sparsely samples the input mossy fiber signal through granule cells, and the inhibition module dynamically modulates the activity level and encoding mode of the granule cells. The feedforward inhibition module averages the mossy fiber signal discharge rate to obtain the activity attribute of Golgi cells, which is inherited to the granule cells. The feedback inhibition module sparsely samples the granule cells to obtain the activity attribute of Golgi cells, which is inherited to the granule cells. The output layer sequentially outputs the multiplication result of the activity range of the granule cells at each time point and the connection weight between the corresponding Purkinje cells as the control cerebellar signal. The application improves the problem that the biological constraint cerebellar network is not accurate and the motion conversion is not smooth in sequence motion continuous learning.

The invention relates to a strategy determination method and electronic equipment, which are used for improving the strategy determination accuracy. Comprising the following steps: inputting a to-be-processed pulse signal into a decision model; wherein the decision model comprises two first modules and a Purkinje cell layer, and any one of the first modules comprises a moss fiber layer and a granular cell layer; for any first module, inputting a to-be-processed pulse signal to the granular cell layer through the moss fiber layer; performing high-dimensional expansion processing and sparse processing on the pulse signal to be processed by using the granular cell layer to obtain an intermediate pulse signal; the Purkinje cell layer is used for prolonging the time scale of instantaneous input stimulation and gradually accumulating stimulation on intermediate pulse signals obtained by the two first modules respectively, and working memory is realized by self-discharge of two groups of Purkinje neurons in the Purkinje cell layer. And strong mutual inhibition between the two groups of Purkinje neurons is utilized to complete a win-win eating process, and a target decision is obtained.

The invention relates to a composition containing stem cells and application of the composition in ovarian aging resistance. According to the invention, the polypeptide TP-TOF with ovarian aging resistance is separated and screened from placenta, and the polypeptide can effectively reduce apoptosis of ovarian granular cells. The polypeptide can effectively promote recovery of ovarian estradiol and follicular poietin levels of rats independently or in combination with stem cells, can remarkably prolong the estrus period, reduce the estrus interval, reduce follicle apoptosis and promote follicle generation, and has a wider application prospect.

The invention discloses an in-vitro stable subculture construction method of a yak ovarian granular cell line. Relates to the technical field of cell culture. Comprising the following steps: carrying out subculture and amplification on primary yak ovarian granular cells to obtain yGCs; and carrying out pCI-neo-hTERT plasmid transfection and establishment of a monoclonal cell. The immortalized yGCs constructed by using an hTERT plasmid method can provide infinitely proliferated homogenized cells, reduce damage to yaks and avoid individual differences, can retain the core function of regulating follicular development of yGCs, provides a high-quality model for analyzing molecular mechanisms such as seasonal oestrus and low reproduction efficiency of yaks, and has theoretical and application values.

The invention relates to an application of an IDH1 gene in porcine ovarian granular cells, in particular to an application in regulating proliferation and apoptosis of the porcine ovarian granular cells and / or steroid hormone synthesis and / or reproductive performance related gene expression. According to the invention, porcine ovarian granular cells are taken as objects, and the expression of the IDH1 gene is regulated to verify that the IDH1 gene is taken as a key target spot for regulating and controlling the functions of the porcine ovarian granular cells so as to influence steroid hormone synthesis and cell proliferation and apoptosis processes. A new molecular target is provided for improving the reproductive performance of the sow, more reliable technical support is provided for regulating and controlling the ovarian function, and a new thought and technical guidance are provided for mechanism research of reproductive diseases and preparation and development of related drugs.

The invention discloses a GV-stage granulosa cell extraction method, which is characterized in that the number of granulosa cells is diluted to 4-6 * 10 < 5 > / L in the extraction process, the extracted cells can be utilized to the maximum extent, PSB is used for flushing in the extraction process, and high-glucose DMEM containing 0.8-1.2% of double antibodies and 8-12% of FBS is used, so that adherent culture of the granulosa cells is neater and cleaner, and the GV-stage granulosa cells are obtained. And adherence and growth of GV-stage granular cells are facilitated.

The invention discloses application of spermidine in preparation of a preparation for resisting oxidative stress injury of ovarian granular cells, and belongs to the technical field of biological medicines. The content of the invention shows that spermidine can improve the ovarian endocrine function and reproductive capacity of oxidative stress mice, normalize the estrus cycle, relieve oxidative stress, increase ovarian reserve and reduce GC cell apoptosis, and verifies that spermidine indeed has the effect of resisting oxidative stress of ovarian granular cells. A thought is provided for solving the problem of oxidative stress of the ovarian granular cells.

The application provides a cost entropy information granulation semi-supervised feature selection method for diabetes detection, and belongs to the technical field of intelligent medical treatment, and the technical scheme is as follows: S1, a weighted granular cell is constructed; S2, a hemispherical granulation is performed on each decision class based on covariance matrix principal direction analysis, and a pseudo label is assigned to an unmarked sample; S3, a cost entropy is introduced for feature evaluation according to feature difference, and feature importance is obtained; S4, the feature importance is sorted, and a feature subset is selected; and S5, the optimal feature subset is obtained by aggregating the feature selection results of each subnode. The application improves the classification accuracy of a multi-dimensional feature diabetes data set, reduces the calculation complexity of a classification method, improves the accuracy and efficiency of feature selection, helps doctors to judge whether a patient is suffering from diabetes, and has strong application value.

The utility model discloses a cell impurity removal vessel which comprises a culture dish body and a culture dish cover, three partition plates are arranged in the culture dish body and divide the culture dish body into three fan-shaped areas, one fan-shaped area is set as a cleaning area, the interior of the cleaning area is divided into a smooth part and a cleaning part, and the smooth part and the cleaning part are arranged in the culture dish body. And concave-convex lines are arranged on the cleaning part. The cell sap can be stored in a partitioned mode, granular cells can be rapidly scraped off from oocytes through the concave-convex lines in the cleaning area during operation, and the cleaning work efficiency of the oocytes is effectively improved.

The invention discloses a method for improving the reproductive performance of cattle by using betaine, and belongs to the technical field of animal reproduction biology. The method comprises applying an effective dose of betaine to bovine oocytes and / or granular cells thereof; the betaine is a natural quaternary ammonium type alkaloid with an anti-oxidation function. According to the invention, it is found through metabonomics that betaine is a key metabolite of cattle high-yield breeding traits for the first time, and it is proved that betaine has double beneficial effects in a cattle breeding system: on a granular cell level, the level of active oxygen in cells induced by hydrogen peroxide can be significantly reduced by adding 5 mM betaine, and oxidative stress can be effectively relieved; on the oocyte level, 0.5 mM betaine is added into an in-vitro maturation and / or embryo culture solution, so that the cleavage rate and blastocyst formation rate of the bovine in-vitro embryo can be remarkably increased. The invention provides a brand-new, safe and effective solution for improving the production efficiency and the reproductive capacity of the bovine in-vitro embryo.

The invention provides an exosome derived from an ovary organ. The invention also provides an extraction method and application of the exosome. According to the method, the differential centrifugation-filtration combined technology is optimized, the in-vitro extraction and identification of the exosome from the human ovary organoid are realized for the first time, and the exosome with high purity and uniform particle size is obtained. The exosome provided by the invention can significantly inhibit abnormal apoptosis of human ovarian granular cells.

The invention provides a novel application of a raspberry extract. It is found through tests that any one of isoquercitrin, ellagic acid, vanillic acid, oleanolic acid, kaempferol-3-O-rutinoside, kaempferol-3-O-sophoroside, tiliroside, tormentic acid and raspberry extract can remarkably improve the activity of the human ovarian granular cells or remarkably improve the oxidative stress level, and the activity of the human ovarian granular cells can be remarkably improved. According to the present invention, the ovarian function can be effectively protected by the eight components, the ovarian function can be effectively protected by the eight components, or the mitochondrial membrane potential level can be significantly regulated and controlled, or the estrogen level in the microenvironment can be effectively maintained, such that the ovarian function can be effectively protected by the eight components, and the raspberry extract containing the eight components. The invention provides a new choice for ovarian function protection products, and also provides a new application for the substances.

The invention belongs to the technical field of medicines, and discloses application of an AMPD3 gene expression inhibitor in preparation of a medicine for preventing, relieving and / or treating polycystic ovarian syndrome. The AMPD3 gene expression inhibitor is a nucleic acid molecule targeting an AMPD3 gene sequence or a recombinant vector containing the nucleic acid molecule. According to the invention, ovarian morphological abnormality and reproductive endocrine metabolism disorder are improved by inhibiting AMPD3 gene expression, reducing ovarian granular cell ferroptosis level, up-regulating GPX4 expression and reducing MDA level. The invention provides a new treatment strategy for patients with polycystic ovarian syndrome.

The invention discloses a male-source female peripheral precocious precocious intervention target based on an imprinting gene Gnas and application of the male-source female peripheral precocious precocious intervention target. The pregestation caffeine exposure of the male parent has obvious influence on the ovarian generation of the female offspring, and can lead to PPP (Poly-Protein Protein) generation. Through screening and verification, it is found that expression increase of the ovarian granular cell imprinting gene Gnas plays a key role in the parent PPP. The cAMP / PKA / CREB signaling pathway can be up-regulated or down-regulated by overexpression of the ovary Gnas of the female rat or knockout of the ovary Gnas of the female offspring based on a parent PPP model, so that the ovary estrogen synthesis function and PPP generation are induced or inhibited. The invention discloses a key effect of the Gnaas / cAMP / PKA / CREB pathway in regulation and control of ovarian estrogen synthesis, and provides a new biological basis and an early prevention and treatment strategy for targeted therapy of female PPP.