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1056 results about "Exosome" patented technology
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Exosomes are extracellular vesicles (EVs) that are produced in the endosomal compartment of most eukaryotic cells. The multivesicular body (MVB) is an endosome defined by intraluminal vesicles (ILVs) that bud inward into the endosomal lumen. If the MVB fuses with the cell surface (the plasma membrane), these ILVs are released as exosomes. In multicellular organisms, exosomes and other EVs are present in tissues and can also be found in biological fluids including blood, urine, and cerebrospinal fluid. They are also released in vitro by cultured cells into their growth medium. Since the size of exosomes is limited by that of the parent MVB, exosomes are generally thought to be smaller than most other EVs, from about 30 to several hundred nanometres (nm) in diameter: around the same size as many lipoproteins but much smaller than cells. Compared with EVs in general, it is unclear whether exosomes have unique characteristics or functions or can be separated or distinguished effectively from other EVs. EVs including exosomes carry markers of cells of origin and have specialized functions in physiological processes, from coagulation and intercellular signalling to waste management. Consequently, there is a growing interest in clinical applications of EVs as biomarkers and therapies alike, prompting establishment of an International Society for Extracellular Vesicles (ISEV) and a scientific journal devoted to EVs, the Journal of Extracellular Vesicles.
Polygonatum sibiricum-wolfberry heterozygous exosome as well as preparation method and application thereof
The invention relates to the field of biological medicine, and discloses a rhizoma polygonati-fructus lycii heterozygous exosome, a preparation method and application thereof and an antioxidant composition. The hybrid exosome comprises miRNA-156a (micro Ribonucleic Acid) and miRNA-396b (micro Ribonucleic Acid), and the expression quantity ratio of the miRNA-156a to the miRNA-396b is (1.2 to 1.8): 1; the heterozygous exosome is loaded with a polygonatum kingianum characteristic active component and a Chinese wolfberry fruit characteristic active component. The rhizoma polygonati-fructus lycii heterozygous exosome is good in biocompatibility and capable of remarkably improving the cell survival rate, and brand-new and efficient raw materials and technical supports are provided for development of anti-aging and anti-oxidation functional products.
Owner:HUNAN ACAD OF CHINESE MEDICINE
Preparation method and application of sea cucumber exosome with immune cell repairing function
ActiveCN121628806ACosmetic preparationsCell dissociation methodsInflammatory factorsPharmaceutical drug
The invention discloses a preparation method and application of a sea cucumber exosome with an immune cell repairing function, and belongs to the technical field of biomedicine. The method comprises the following steps: feeding sea cucumbers by using a combined method of composite stress and an echinacea extract, extracting sea cucumber cells, and obtaining sea cucumber exosomes through ultracentrifugation; wherein the condition of composite stress is set as follows: the day and night temperature difference is not lower than 3 DEG C, and meanwhile, the dissolved oxygen content of the water body is adjusted to 75-90% of the dissolved oxygen content of the conventional feeding water body. The obtained sea cucumber exosome has a remarkable immune cell repairing function, and can effectively promote proliferation and activity recovery of immune cells, inhibit expression of inflammatory factors and improve the level of anti-inflammatory factors. The sea cucumber exosome prepared by the preparation method disclosed by the invention has an important application prospect in the research and development of immune repair drugs, and can provide a technical support for a novel immune regulation preparation in the field of biomedicine.
Owner:ZHONGPEPTIDE GUODA (BEIJING) BIOTECHNOLOGY CO LTD
Exosome RNA detection composition based on positive and negative targets and use method thereof
PendingCN121362841AMicrobiological testing/measurementDNA/RNA fragmentationReverse transcriptaseMicrobiology
The invention belongs to the field of biological detection, and discloses an exosome RNA detection composition based on positive and negative targets and a use method thereof, the composition comprises two positive targets and one negative target: the positive target A is miRNA stably expressed in exosome and is used for reflecting the expression level of small RNA; the positive target B is typical mRNA and is used for evaluating the quality of the long-fragment RNA; the negative target C is bacterial 16S rRNA and is used for identifying bacterial nucleic acid pollution in a sample. The method combines III-generation reverse transcriptase and HotStart Taq enzyme to construct a qPCR system, has high sensitivity and specificity, and is suitable for rapid detection of trace RNA. Whether the exosome RNA is suitable for downstream analysis or not can be judged by acquiring the Cq values of the three types of targets and setting a reference range. According to the invention, the integration of quality control, quantification and pollution identification of the exosome RNA is realized.
Owner:WISDELIVERY BIOTECHNOLOGY (WUHAN) CO LTD
Implant exosome and application thereof in inflammation resistance, oxidation resistance and bacterium resistance
The invention discloses an implant-derived exosome which is prepared by the following steps: adding cleaned and crushed colored rice flour into a PBS (Phosphate Buffer Solution) with the pH value of 6-7.4, homogenizing at a high speed, adding alpha-amylase into rice milk, performing enzymolysis for 1-2 hours under the conditions that the pH value is 6-7.4 and the temperature is 90-97 DEG C, filtering, and standing filtrate overnight; carrying out gradient differential centrifugation at 4 DEG C, collecting supernate, filtering, carrying out ultracentrifugation on filtrate at 4 DEG C and 12000-15000g for 1.5-3 hours, and collecting precipitate; and re-suspending the precipitate, enriching by using an MWCO ultrafiltration tube with the molecular weight cut-off of 100kDa, collecting the precipitate at the bottom of the ultrafiltration tube, and re-suspending in a PBS buffer solution, thereby obtaining the product. The exosome can effectively inhibit the generation of inflammatory factors, has a good inhibition effect on various pathogenic bacteria, also has an anti-oxidation effect, and has application prospects in the fields of inflammation resistance, bacteria resistance, oxidation resistance and the like.
Owner:YUNNAN MINZU UNIV
Engineering bionic nucleic acid nano-vesicle as well as preparation method and application thereof
The invention discloses an engineered bionic nucleic acid nano-vesicle as well as a preparation method and application thereof, relates to the technical field of nano biomedicine, and aims at solving the problems that in-vivo targeting efficiency and immunogenicity of a traditional cationic lipid nano-carrier are limited due to deletion and cleavage obstacles of GSDMD expression in tumor cells. The technical key point of the invention is as follows: the engineered bionic nucleic acid nano-vesicle is provided and is prepared by wrapping a cationic lipid nucleic acid drug with an exosome derived from engineered macrophages; wherein the exosome from the engineered macrophage is the exosome from the macrophage with high expression of PD1, which is as shown in SEQ. ID. NO.1. The exosome from the engineered macrophage is the exosome from the macrophage with high expression of PD1; the lipid nucleic acid medicine is prepared by loading GSDMD-N mRNA (messenger Ribonucleic Acid) shown on the basis of SEQ.ID.NO.2 on a cationic liposome. The engineered bionic nucleic acid nano-vesicle is used for preparing an oral squamous cell carcinoma diagnostic kit and a therapeutic drug.
Owner:HARBIN MEDICAL UNIVERSITY
Double-crosslinking hydrogel loaded with stem cell exosome as well as preparation and application of double-crosslinking hydrogel
PendingCN121221846APeptide/protein ingredientsInorganic phosphorous active ingredientsCyclodextrinBiomedicine
The invention relates to the technical field of biomedicine, in particular to stem cell exosome-loaded bi-crosslinked hydrogel as well as preparation and application thereof, and the bi-crosslinked hydrogel comprises the following components in parts by weight: 50-90 parts of a matrix polymer; 0.1 to 5 parts of an active load; 1 to 10 parts of cyclodextrin; 5 to 20 parts of modified chitosan; 0.01 to 1 part of a photoinitiator; and 0.01 to 0.5 part of growth factors. Through double-network design of physical / chemical primary cross-linking and photo-initiation secondary cross-linking, the bottleneck that the mechanical property and the machinability of the traditional single cross-linking hydrogel are difficult to consider at the same time is overcome; meanwhile, the human umbilical vein endothelial cell exosome is introduced, and efficient loading, uniform dispersion and degradation coupling slow release of the exosome are realized by virtue of beta-cyclodextrin inclusion and a double-crosslinking network. Therefore, the problems of mechanics, degradation, cross-linking speed or tissue adhesion of cross-linked hydrogel in the prior art are solved.
Owner:BEIJING UNIV OF CHEM TECH
High-activity injectable self-healing temperature-sensitive hydrogel dressing as well as preparation method and application thereof
The invention discloses a high-activity injectable self-healing temperature-sensitive hydrogel dressing and a preparation method and application thereof, the high-activity injectable self-healing temperature-sensitive hydrogel dressing is obtained by forming a temperature-sensitive hydrogel through a two-dimensional nano material MXene aqueous solution and F127 under the hydrogen-bond interaction, combining an M2 macrophage source exosome on MXene under the electrostatic adsorption action, and wrapping the MXene through a network structure of the hydrogel. The preparation method is simple, an obtained sample is free of organic solution residues, reaction conditions are mild, operation is convenient, the raw material cost is low, the hydrogel dressing has temperature-sensitive, injectable and self-healing performance, the hydrogel dressing has temperature-sensitive and injectable performance, is sol at normal temperature, forms gel after making contact with skin and is convenient to smear and use on wounds, and experimental results prove that the hydrogel dressing has good application prospects. The hydrogel has good biocompatibility and relatively strong antibacterial performance, and can effectively play anti-inflammatory and vascular regeneration promoting roles in diabetic wounds and promote rapid repair of the diabetic wounds.
Owner:THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
Application of stem cell exosome in treatment of depression-like behavior caused by high-altitude hypoxia
The invention discloses application of a stem cell exosome in treating depression-like behaviors caused by high-altitude hypoxia, and belongs to the technical field of biomedicine. The stem cell exosome is an exosome derived from human umbilical cord mesenchymal stem cells, and is used for drugs for preventing and / or treating depression-like behaviors caused by high-altitude hypoxia. The exosome can effectively improve depression-like core behavior symptoms caused by high-altitude hypoxia by regulating a brain-intestinal axis; the method starts from remodeling intestinal flora so as to influence the intracerebral metabolite spectrum, and finally plays a role in neuroprotection by regulating and controlling a 5-HT6 / cAMP-PKA key signal channel. In addition, the combination of the hUC-MSC-exo and probiotics shows synergistic interaction potential, and a potential treatment strategy which is novel in mechanism, remarkable in effect and high in safety is provided for the field.
Owner:QINGHAI UNIVERSITY
Modified pseudo-ginseng exosome as well as intestinal conditioning gel composition and application thereof
The invention relates to the technical field of functional food and biological medicine, in particular to a modified pseudo-ginseng exosome and an intestinal conditioning gel composition and application thereof. According to the gel beverage, an exosome derived from panax notoginseng callus cultured in a laboratory is used as a core active component, is modified through phosphatidylserine-aminated pectin-genipin and is embedded into a hydrogel matrix composed of carboxymethyl chitosan and sodium alginate, so that the exosome is protected from being degraded in the stomach, and the activity of the exosome in the stomach is improved. The exosome can be effectively released under the intestinal pH and flora environment, so that the targeted delivery of the intestinal tract is realized, and the preparation method is suitable for improving the intestinal inflammation and maintaining the intestinal health. The invention can effectively solve the technical problems of uncontrollable raw material quality and safety, low oral delivery stability and active ingredient availability and poor medication compliance of the existing plant exosome.
Owner:JIANGSU JICUI FUNCTIONAL MATERIALS RES INST CO LTD
Injectable temperature-sensitive hydrogel loaded with traditional Chinese medicine active exosome
The invention relates to injectable temperature-sensitive hydrogel loaded with traditional Chinese medicine active exosomes, and belongs to the technical field of clinical treatment of diabetic foot refractory wounds, and the injectable temperature-sensitive hydrogel is obtained by poloxamer 407 hydrogel and a hyaluronic acid loaded composite carrier; the composite carrier body is formed by coating a traditional Chinese medicine active exosome with a coating solution; the traditional Chinese medicine active exosome is obtained by inducing adipose-derived mesenchymal stem cells with a modified astragalus extract. The injectable temperature-sensitive hydrogel is obtained by loading a composite carrier with poloxamer 407 hydrogel and hyaluronic acid, the poloxamer 407 and the hyaluronic acid quickly form a physical barrier on a wound surface after being injected, a moist healing environment is provided, and continuous and slow release of exosome and active ingredients is realized by means of the temperature-sensitive characteristic and the network structure of the poloxamer 407 and the hyaluronic acid, so that the wound healing effect is improved. And over-high local concentration or premature loss caused by sudden release of the medicine is avoided.
Owner:SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
Temperature-sensitive hydrogel loaded with mesenchymal stem cell exosome as well as preparation method and application of temperature-sensitive hydrogel
The invention discloses a temperature-sensitive hydrogel loaded with mesenchymal stem cell exosome as well as a preparation method and application of the temperature-sensitive hydrogel, and belongs to the technical field of biological medicines. The method comprises the following steps: (1) adding poloxamer 407 into a pre-cooled dispersion medium to enable the final mass concentration to be 20-30%, and oscillating and dissolving at 4 DEG C overnight to obtain poloxamer hydrogel; (2) collecting a mesenchymal stem cell culture supernatant, and then centrifuging, wherein the centrifuging condition is 200 * g * 15 minutes to 2000 * g * 20 minutes to 12000 * g * 20 minutes; filtering the supernatant, concentrating by using an ultrafiltration tube, further performing ultracentrifugation on the concentrated solution, re-suspending the precipitate by using DPBS, centrifuging again, and re-suspending the finally obtained exosome precipitate by using DPBS to obtain an exosome suspension; the ultracentrifugation condition is as follows; and (3) taking the poloxamer hydrogel prepared in the step (1), adding the exosome suspension obtained in the step (2) under an ice-water bath condition, and uniformly mixing to obtain the poloxamer temperature-sensitive hydrogel.
Owner:SOUTHEAST UNIV +1
Gelatin derivative microsphere coated with stem cell exosome, preparation method of gelatin derivative microsphere and application of gelatin derivative microsphere in preparation of external ophthalmic preparation
The invention provides gelatin derivative microspheres coated with stem cell exosomes, a preparation method of the gelatin derivative microspheres and application of the gelatin derivative microspheres in preparation of external ophthalmic preparations, and relates to the technical field of medicines. The gelatin derivative microsphere mainly comprises a microsphere core and a shell, wherein the microsphere core is a stem cell exosome coated with a gelatin derivative; the shell is obtained by crosslinking a gelatin derivative and sodium hyaluronate. The gelatin derivative used in the invention not only has good compatibility with the stem cell exosome, but also has good dispersibility in tears or periorbital secretion after being prepared by crosslinking the gelatin derivative and sodium hyaluronate, so that the elimination of an eye protection mechanism to the microspheres can be reduced, and the slow release effect of the exosome can be improved; besides, the shell formed by the hyaluronic acid has a very good tackifying effect, so that the retention time of the gelatin derivative hyaluronic acid microspheres in eyes is prolonged, and the problem of burst release of the medicine is also avoided by virtue of the double-layer coated microsphere structure.
Owner:JILIN HUIRONG BIOTECHNOLOGY CO LTD
Exosome freeze-dried powder as well as preparation method and application thereof
The invention belongs to the technical field of exosome freeze-dried powder, and discloses exosome freeze-dried powder and a preparation method thereof, the exosome freeze-dried powder contains mesenchymal stem cell exosome, trehalose, sodium hyaluronate, hydroxypropyl-beta-cyclodextrin, glutathione, ceramide and fructo-oligosaccharide. According to the exosome freeze-dried powder, hydroxypropyl-beta-cyclodextrin, glutathione, ceramide and fructo-oligosaccharide are added, the membrane structure integrity of the exosome is effectively protected, the retention rate of active substances in the exosome freeze-dried powder is high, and therefore the effects of resisting inflammation, regulating cell metabolism, promoting tissue repair and the like of the exosome can be fully exerted. In addition, glutathione can directly remove skin free radicals and inhibit oxidative stress injury, and has an anti-oxidation effect; ceramide can also supplement skin lipid barrier, enhance the water locking capacity and enhance the moisturizing effect of sodium hyaluronate; fructo-oligosaccharide can also regulate skin micro-ecological balance, and has anti-inflammatory and bacteriostatic effects.
Owner:GUANGZHOU EXOSOME BIOTECHNOLOGY CO LTD
HA-PAS hydrogel for enhancing cartilage repair of early osteoarthritis and preparation method of HA-PAS hydrogel
The invention discloses HA-PAS hydrogel for enhancing cartilage repair of early osteoarthritis and a preparation method of the HA-PAS hydrogel. According to the hydrogel, formylated hyaluronic acid, hydrazide modified low-methoxyl pectin and phenylboronic acid modified alginate form a ternary polysaccharide skeleton, a double-dynamic cross-linked network is formed through acylhydrazone bonds and boric acid ester bonds, and a triple network structure is formed by combining permanent cross-linking catalyzed by horse radish peroxidase. The hydrogel integrates functional components such as nano-selenium, a pulullan-strontium chelate and a mesenchymal stem cell source exosome, so that multiple effects of resisting oxidation, resisting inflammation, promoting cartilage differentiation and repairing subchondral bones are achieved. The compression modulus of the hydrogel is 0.8-1.5 MPa, and the hydrogel can be injected for in-situ gelling and MMP responsive degradation, shows an excellent cartilage repair effect in animal experiments, and provides a new solution for treatment of early osteoarthritis.
Owner:THE FIRST HOSPITAL OF LANZHOU UNIV
Mesenchymal progenitor cells for enhancing partial reprogramming of target cells
Provided herein are methods and related compositions for enhancing or enhanced partial reprogramming of target cells in a subject in need thereof (e.g., a human subject suffering from or at risk of a disease), the method comprising administering a plurality of mesenchymal lineage progenitor or stem cells (MLPSCs), exosomes derived therefrom, or conditioned culture media derived therefrom to a subject that expresses or will express one or more reprogramming factors in a population of target cells, whereby a plurality of the target cells in the subject become partially reprogrammed, but not fully reprogrammed.
Owner:MESOBLAST INTERNATIONAL SARL
Method for preparing allergic rhinitis spray from umbilical cord mesenchymal stem cell exosome
PendingCN121570497ACell dissociation methodsPeptide/protein ingredientsMesenchymal stem cellPlatelet
The invention relates to the technical field of rhinitis spray preparation, in particular to a method for preparing allergic rhinitis spray from umbilical cord mesenchymal stem cell exosomes, which comprises the following steps: 1, umbilical cord treatment; 2, culturing umbilical cord mesenchymal stem cells; 3, preparing an umbilical cord mesenchymal stem cell exosome; and 4, preparing the rhinitis spray. The rhinitis spray prepared by the method disclosed by the invention is simpler and more convenient to use. The exosome, the platelet growth factor and other substances in the rhinitis spray well solve the core problems of single curative effect, large side effect, low compliance and the like in the traditional rhinitis treatment, and through the induction and repair functions of the stem cell exosome and the bactericidal ability of the antibacterial peptide, the nasal cavity environment is improved, the anaphylactic reaction is reduced, the nasal mucosa is repaired, and the rhinitis treatment effect is improved. The allergic rhinitis is expected to be fundamentally treated.
Owner:山西药科职业学院
Artemisia annua cell-derived exosome nano-vesicle and application of inclusion peroxidase A0A2U1N9S9 of exosome nano-vesicle in preparation of anti-colorectal cancer drugs
The invention discloses an application of exosome nano-vesicles derived from artemisia annua cells and peroxidase A0A2U1N9S9 contained in the exosome nano-vesicles in preparation of anti-colorectal cancer drugs. According to the present invention, the anti-CRC activity analysis results show that the artemisia annua cell-derived exosome nano-vesicles can inhibit the growth of DLD-1 and HCT116 cell strains, and the active substance base of ACDENVs for inhibiting the growth of DLD-1 and HCT116 cell strains, namely peroxidase A0A2U1N9S9, is innovatively revealed for the first time based on the difference analysis of the proteomics technology; and a new research thought and a theoretical basis are provided for the research of anti-colorectal cancer drugs.
Owner:JINAN UNIVERSITY
Sleep-promoting nasal spray based on mesenchymal stem cell source exosome as well as preparation and application of sleep-promoting nasal spray
The invention discloses a sleep-promoting nasal spray based on mesenchymal stem cell-derived exosomes and preparation and application thereof.The preparation method comprises the steps that 1, mesenchymal stem cells separated from umbilical cord tissue are cultured to P3-P5 generations, cell culture supernate is collected, cells are digested, and then cell debris and macromolecular impure protein are removed through pretreatment; (2) filtering and concentrating the pretreated cell culture supernatant and replacing a part of solution; (3) carrying out degerming filtration on the solution obtained in the step (2) through a 0.22 [mu] m filter membrane to obtain an MSC-Exos solution; and (4) adding a stabilizer with a proper concentration into the MSC-Exos solution to obtain a compound MSC-Exos solution, namely the sleep-promoting nasal spray. The product is a cell-derived natural active substance, can significantly improve sleep, and does not involve any pharmaceutical ingredient.
Owner:GUANGZHOU SHAOYUAN BIOTECHNOLOGY CO LTD
Abelmoschus manihot exosome extraction process
The invention discloses an abelmoschus manihot exosome extraction process, and relates to the field of extraction and purification in biotechnology, and the abelmoschus manihot exosome extraction process comprises the following steps: (1) providing a fresh flower material of abelmoschus manihot, and carrying out mechanical cleaning and surface impurity removal; and (2) homogenizing the cleaned fresh flowers of the abelmoschus manihot for 5-30 minutes by a high-shear homogenizer under the condition that the rotating speed is 8000-15000rpm to obtain homogenate. The method is specially designed for the physiological characteristics of the fresh flowers of the abelmoschus manihot, and through the core principle of'rapid treatment and low-temperature operation ', the activity loss of the exosomes in the extraction process is reduced to the greatest extent, so that the yield of the exosomes is increased, and the yield of the abelmoschus manihot is increased. The method ensures the integrity of the natural structure and function, creatively adopts a process route of combining continuous flow centrifugation and multistage tangential flow ultrafiltration, realizes continuous and efficient separation and purification of exosomes from a large number of plant raw materials, solves the industrial bottleneck that a traditional ultracentrifugation method cannot realize large-scale production, and is suitable for industrial production. And the treatment capacity can easily reach 5-15 tons of fresh flowers per day.
Owner:ZHUANGJIAN (CHONGQING) BIOTECHNOLOGY CO LTD
Application of epidermal growth factor-loaded pseudo-ginseng exosome-like nano-vesicles in wound healing
The invention discloses a pseudo-ginseng exosome-like nano vesicle loaded with an epidermal growth factor / application of the pseudo-ginseng exosome-like nano vesicle in wound healing. The research shows that not all exosome-like nano-vesicles from Chinese herbal medicines capable of promoting wound healing have the effect of promoting wound healing, the action mechanism of the pseudo-ginseng exosome-like nano-vesicles in wound healing is further explored, and the research shows that PNVs can promote the proliferation and migration of an epidermal keratinocyte line, so that the PNVs can be used for promoting the healing of the wound. The treatment can be realized by regulating and controlling the activity of immune cells to promote cell proliferation and migration, and the wound healing effect of the EGF-loaded EGF-coated PNVs is remarkably enhanced, so that the EGF-loaded EGF-coated PNVs have a synergistic effect. The invention not only provides a theoretical basis for deeply researching PELNVs, but also provides a new method and a new idea for treating acute wounds.
Owner:THE SEVENTH AFFILIATED HOSPITAL SUN YAT SEN UNIV SHENZHEN
Application of 3D hypoxia mesenchymal stem cell derived exosome preparation in preparation of immune cell proliferation inhibitor or medicine for treating acute myocardial infarction
The invention discloses application of a 3D hypoxia mesenchymal stem cell derived exosome preparation in preparation of an immune cell proliferation inhibitor or a medicine for treating acute myocardial infarction, belongs to the technical field of biology, and solves the problems of low cell survival rate and immunological rejection in application of a cell treatment method in the prior art. The 3D hypoxia mesenchymal stem cell derived exosome is used as a medium for treating acute myocardial infarction, and the problem of immunological rejection can be solved.
Owner:SHANGHAI TONGJIN STEM CELL TECHNOLOGY CO LTD
Astragaloside-loaded astragalus membranaceus exosome, preparation method thereof and application of astragalus membranaceus exosome in preparation of medicine for treating cerebral ischemia-reperfusion injury
PendingCN121401238AOrganic active ingredientsPharmaceutical non-active ingredientsPharmacy medicinePharmaceutical drug
The invention relates to an astragaloside-loaded astragalus membranaceus exosome which is prepared by the following steps: crushing astragalus membranaceus, and filtering to obtain juice; performing multi-step differential centrifugation to obtain supernate; carrying out ultrafiltration and centrifugation, and collecting the precipitate; adding to the upper layer of a sucrose stepped gradient solution, centrifuging, and collecting part of strips; after dilution, centrifuging, discarding the supernatant, precipitating, and removing cane sugar to obtain the astragalus membranaceus exosome; the radix astragali exosome loaded with the astragaloside is obtained by mixing the radix astragali exosome with the astragaloside and carrying out ultrasonic treatment or co-incubation, and can be used for preparing medicines for specifically inhibiting cGAS / STING pathway excessive activation in cerebral ischemia reperfusion injury and downstream NLRP3 inflammasome mediated pyroptosis and preparing medicines for protecting and repairing mitochondria in the cerebral ischemia reperfusion injury. The homologous astragalus membranaceus exosome is used as a natural carrier, the blood-brain barrier is effectively overcome, and the enrichment concentration and bioavailability of astragaloside in a cerebral ischemia area are remarkably improved.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY
New use of exosome secreted by hepatocyte lineage cells differentiated via directed induction of embryonic stem cells
The present invention relates to the new use of an exosome secreted by hepatocyte lineage cells differentiated via the directed induction of embryonic stem cells, and in particular relates to the use of the exosome in the preparation of a drug for treating liver diseases. The exosome is secreted by hepatocyte lineage cells differentiated via the directed induction of embryonic stem cells, and the hepatocyte lineage cells are hepatic progenitor cells and / or mature hepatocyte-like cells.
Owner:GUANGXIU GAOXIN LIFE SCIENCES CO LTD HUNAN
Culture method for improving proliferation and differentiation capacity of NK (Natural Killer) cells based on angelica sinensis-astragalus membranaceus exosomes
PendingCN121249584AMammal material medical ingredientsAntiinfectivesBiotechnologyAngelica Sinensis Root
The invention discloses a culture method for improving proliferation and differentiation capacity of NK (Natural Killer) cells based on angelica sinensis-astragalus membranaceus exosomes, which comprises the following steps: firstly extracting exosomes in angelica sinensis and astragalus membranaceus, then adding the exosomes into an NK cell culture system according to a specific concentration, and regulating and controlling an NK cell signal channel by virtue of active ingredients carried by the exosomes so as to improve the proliferation and differentiation capacity of the NK cells. Therefore, the proliferation rate and differentiation maturity of the NK cells are remarkably improved. Experimental results show that compared with a traditional culture method, the proliferation multiple of the NK cells cultured through the method is increased by 28.99%-33.69%, the proportion of differentiated mature cells is increased by 15.91%-28.30%, and the cell killing activity is not remarkably reduced. The method can be widely applied to the field of immune cell treatment, provides a high-quality NK cell source for clinic, effectively solves the problems of slow cell proliferation rate, low differentiation maturity, easy activity reduction after large-scale culture and the like in traditional NK cell in-vitro culture, and has important clinical application value and industrialization prospect.
Owner:HENAN TISSUE CELL BANK CO LTD
Method and system for predicting early gastric cancer prognosis by circulating marker
The invention provides a method and a system for predicting early gastric cancer prognosis by a circulating marker, and relates to the technical field of auxiliary diagnosis. The method comprises the following steps: performing multi-omics detection on a blood sample based on a preset sampling time sequence to obtain a multi-dimensional time sequence characteristic data set containing three groups of heterogeneous data of circulating tumor DNA, exosomes and protein markers; calculating a change slope and a fluctuation variance of the heterogeneous data in adjacent time sequence intervals, constructing a dynamic variation feature matrix in combination with a standard attenuation weighting factor, and deeply mining spatial cross-correlation and sequence dependence features of the matrix to generate a multi-modal fusion feature fingerprint; and performing regression operation on the feature fingerprints by using an integrated learning stack model to obtain a dynamic prognosis risk score, and further retrieving a risk hierarchical mapping table to generate a prognosis evaluation result containing a survival curve. According to the method, multi-modal heterogeneous data can be effectively fused, the biological dynamic characteristics in the tumor postoperative recovery phase are captured, and the accuracy and timeliness of early gastric cancer prognosis prediction are remarkably improved.
Owner:THE FIRST MEDICAL CENT CHINESE PLA GENERAL HOSPITAL
Preparation method of high-activity exosome hair growth promoter
The invention discloses a preparation method of a high-activity exosome hair growth promoter, which comprises the following steps: providing purified exosome, and mixing the purified exosome with hair growth active ingredients in a PBS (Phosphate Buffer Solution) with the pH value of 7.0-7.6, adding hydroxypropyl-beta-cyclodextrin with the final concentration of 0.5%-2% (w / v) and trehalose with the final concentration of 5%-10% (w / v), mildly incubating for 1-2 hours under the conditions that the temperature is 35-37 DEG C and the speed is 50-100 rpm, purifying, and freeze-drying to obtain a solid product. According to the invention, through the synergistic effect of the specific membrane permeability enhancer and the membrane stabilization protective agent, the efficient loading of the hair growth active component and the natural biological activity of the exosome are considered, the process is mild and controllable, the industrial amplification is easy, and the solid preparation solves the problem of inconvenient storage and transportation, and has strong practicability.
Owner:JINZHANLI TECHNOLOGY DEVELOPMENT CO LTD
Preparation method and application of circular RNA (Ribonucleic Acid) and targeting gene engineering exosome
An iRGD-LAMP2B-EGFP fusion protein mediated exosome surface engineering strategy is adopted, through specific recognition of iRGD peptide and integrin alpha v beta 3, corneal neovascularization core lesion cells are actively targeted, the limitation of off-target toxicity of a traditional delivery system is broken through, and a'accurate focus recognition-efficient homing 'targeted delivery normal form is established. Meanwhile, the screened circular RNA with anti-inflammatory and anti-angiogenesis dual activities is used as a core effector molecule, and in combination with an in-situ loading technology of the circular RNA molecule in an exosome parent cell, stable entrapment and targeted release of a nucleic acid drug in an exosome cavity are realized, and the problems that the nucleic acid drug is easy to degrade and poor in targeting property are solved; and dual guarantee of function specificity and delivery stability is formed. And a synergistic treatment system of a targeting exosome carrier and bifunctional circular RNA is further constructed, active molecules are accurately delivered to focus cells, and an inflammation microenvironment and an angiogenesis pathway are synchronously regulated and controlled.
Owner:THE FIRST AFFILIATED HOSPITAL HENGYANG MEDICAL SCHOOL UNIV OF SOUTH CHINA
Exosomes and micro-ribonucleic acids for tissue regeneration
Several embodiments relate to methods of repairing and / or regenerating damaged or diseased tissue comprising administering to the damaged or diseased tissues compositions comprising exosomes. In several embodiments, the exosomes comprise one or more microRNA that result in alterations in gene or protein expression, which in turn result in improved cell or tissue viability and / or function.
Owner:CEDARS SINAI MEDICAL CENT
Preparation method of probiotic and exosome preparation applied to immunoregulation
The invention relates to the technical field of bioengineering, in particular to a preparation method of a probiotic and exosome preparation applied to immunoregulation. The probiotics and the umbilical cord stem cell exosome are combined for use, the immune regulation effect is enhanced from multiple targets and multiple ways, the immune function of the body is improved, the exosome carries a specific molecular marker and acts on immune cells in a targeted mode, precise immune regulation is achieved, and the preparation is in an oral form and convenient to use; the exosome is extracted from umbilical cord stem cells, has no pathogenicity and immunogenicity, and is low in adverse reaction risk after being used for a long time, and the prepared combined preparation can be used for treating and preventing various immune-related diseases, and also can be used for daily conditioning of people with low immunity and improving the body resistance; meanwhile, the combined preparation adopts micro-capsule embedding, so that the probiotics can be effectively protected from gastric acid and bile erosion, the survival rate and colonization efficiency of the probiotics in intestinal tracts are improved, the immunoregulation effect is further enhanced, and the combined preparation is convenient to store and transport and has a good application prospect.
Owner:FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA
Exosome separation and concentration method using marine animal source tissue as raw material
PendingCN121825849ABioreactor/fermenter combinationsShaking/oscillating/vibrating mixersBiotechnologyTissue sample
The invention relates to the technical field of bioengineering, in particular to an exosome separation and concentration method with marine animal source tissue as a raw material. Comprising the following steps: freeze-drying and crushing a marine animal source tissue sample; performing sample enzymolysis; and removing impurities and concentrating. According to the method disclosed by the invention, the exosome in the cells is fully dissociated through freeze-dried powder crushing and composite enzymolysis processes, and the filtering speed of the sample is accelerated by virtue of ultrasonic oscillation, so that the rapid and high-purity separation and concentration of the exosome in the marine animal source tissue are realized.
Owner:OCEAN UNIV OF CHINA +1