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2655 results about "Exosome" patented technology
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Exosomes are extracellular vesicles (EVs) that are produced in the endosomal compartment of most eukaryotic cells. The multivesicular body (MVB) is an endosome defined by intraluminal vesicles (ILVs) that bud inward into the endosomal lumen. If the MVB fuses with the cell surface (the plasma membrane), these ILVs are released as exosomes. In multicellular organisms, exosomes and other EVs are present in tissues and can also be found in biological fluids including blood, urine, and cerebrospinal fluid. They are also released in vitro by cultured cells into their growth medium. Since the size of exosomes is limited by that of the parent MVB, exosomes are generally thought to be smaller than most other EVs, from about 30 to several hundred nanometres (nm) in diameter: around the same size as many lipoproteins but much smaller than cells. Compared with EVs in general, it is unclear whether exosomes have unique characteristics or functions or can be separated or distinguished effectively from other EVs. EVs including exosomes carry markers of cells of origin and have specialized functions in physiological processes, from coagulation and intercellular signalling to waste management. Consequently, there is a growing interest in clinical applications of EVs as biomarkers and therapies alike, prompting establishment of an International Society for Extracellular Vesicles (ISEV) and a scientific journal devoted to EVs, the Journal of Extracellular Vesicles.
Extraction and purification method of plant-derived exosome, plant-derived exosome and application of plant-derived exosome
The invention discloses an extraction and purification method and application of plant-derived exosome-like nanoparticles, and relates to the technical field of plant exosome preparation. The extraction and purification method comprises the following steps: washing fresh ginseng, airing for later use, adding a proper amount of buffer solution, mixing, breaking walls, filtering, centrifuging filtrate to obtain supernate, carrying out ultracentrifugation on the supernate to obtain ginseng exosome nano-particle precipitates, resuspending the precipitates, respectively adding pure water sucrose solutions with different concentrations, and carrying out ultracentrifugation to obtain ginseng exosome nano-particles; and re-suspending the target strip, and carrying out ultracentrifugation to remove redundant cane sugar so as to obtain the purified ginseng exosome-like nano-particles. The ginseng exosome-like nano-particles disclosed by the invention have a remarkable effect when being applied to cosmetics or medicines with skin photoaging resistance, skin oxidation resistance and wrinkle resistance. In an anti-UVB (Ultraviolet B) induced cell injury experiment, the ginseng exosome-like nano-particles have an excellent anti-UVB induced cell injury effect.
Owner:INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI +1
Exosome-containing eye drops as well as preparation method and application thereof
The invention relates to eye drops containing exosomes and a preparation method and application thereof, and belongs to the technical field of eye drop preparation, the eye drops comprise exosome freeze-dried powder, the exosome freeze-dried powder is prepared from the exosomes obtained after mesenchymal stem cells are treated with TNF-alpha and IFN-alpha, and a herba houttuyniae extract, the eye drops provided by the invention can inhibit the phenotype of macrophage M1 and promote the M1 to be converted into the phenotype of M2; human corneal cell proliferation, migration and damage repair are promoted; mouse cornea injury repair is accelerated; the eye drops can be used for treating xerophthalmia, conjunctivitis and keratitis, and can also be used for preventing eye diseases and relieving asthenopia; the eye drops disclosed by the invention contain the exosome obtained after the mesenchymal stem cells are treated by the TNF-alpha and the IFN-alpha, so that the anti-inflammatory ability is stronger; the mesenchymal stem cell exosome and the herba houttuyniae extract are stored in a freeze-dried form, so that the activity is stable, and the storage time is long; in addition, the cordate houttuynia is cooperated with the exosome, so that the effect playing time can be prolonged, and the use frequency is reduced.
Owner:MIWAIMI (ZHANGJIAGANG) BIOTECHNOLOGY CO LTD +1
Composition with effects of protecting liver and resisting oxidation and preparation method thereof
InactiveCN120713968AHydroxy compound active ingredientsInorganic phosphorous active ingredientsBiotechnologyChitosan nanoparticles
The invention relates to the technical field of pharmaceutical preparations, and discloses a composition with liver protection and antioxidation effects and a preparation method thereof.The composition is prepared from galactosamine modified astragalus membranaceus exosome, schisandra chinensis nanocrystals, liquorice-chitosan nanoparticles, lactobacillus acidophilus freeze-dried powder, beta-(1, 3) / (1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate) sourced from saccharomyces cerevisiae, and beta-(1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate). And (6) a dextran, grape exosome-curcumin hybrid vesicle freeze-dried powder, a curcumin-mitochondrial targeting peptide compound, a resveratrol-hydroxypropyl-beta-cyclodextrin inclusion compound and reduced panthenol. According to the liver-protecting and anti-oxidation composition disclosed by the invention, multi-dimensional intervention on liver protection is realized through an elaborately designed ternary system. The system is composed of a traditional Chinese medicine extract, a microorganism-plant exosome combination and a high-bioavailability antioxidant, all the parts have a synergistic effect, and the liver protection effect is remarkably improved.
Owner:BEIJING FUTAIMING BIOTECHNOLOGY CO LTD
Stem cell exosome vesicles induced by traditional Chinese medicine functional components as well as preparation method and application of stem cell exosome vesicles
The invention provides a stem cell exosome vesicle induced by traditional Chinese medicine functional components as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. According to the preparation method of the stem cell exosome vesicle, traditional Chinese medicine functional components serve as an exosome inducer, stem cells can be induced to proliferate and secrete exosome with the enhanced function, the yield of the exosome can be increased, bioactive molecules can be loaded, and the bioavailability of the exosome in diabetes treatment is remarkably improved. The stem cell exosome vesicle disclosed by the invention can be applied to treatment of diabetes and complications thereof, not only can improve functions of islet cells, but also can treat chronic inflammation, microangiopathy and other metabolic diseases related to diabetes, and has a wide clinical application prospect.
Owner:北京圣美细胞生命科学工程研究院有限公司
Exosome, mesenchymal stem cell and application of exosome and mesenchymal stem cell in treatment of type 2 diabetes mellitus
The invention provides an exosome, a mesenchymal stem cell and application of the exosome and the mesenchymal stem cell in treatment of type 2 diabetes mellitus, and belongs to the technical field of biological medicine. The exosome secreted by the umbilical cord mesenchymal stem cells of the composite pretreatment group is used for treating the type 2 diabetes mellitus, and the obvious advantages are shown. Compared with a model control group, the exosome has the advantages that the glucose tolerance of diabetic rats treated by the exosome is obviously improved, the blood glucose value is reduced by about 47.5% within 120 minutes, the area under a blood glucose curve (AUC) is reduced by about 50.5%, the serum insulin is increased by about 147.7%, insulin secretion is effectively promoted, the glycosylated hemoglobin level is obviously reduced, and the long-term blood glucose control effect is good. By integrating various indexes, the exosome obtained by the composite pretreatment scheme provided by the invention has a remarkable effect of improving the illness state of rats with type 2 diabetes mellitus, provides a new effective strategy for treatment of type 2 diabetes mellitus, and shows a good application prospect.
Owner:WEST CHINA HOSPITAL SICHUAN UNIV +1
Application of camellia nitidissima exosome-like nano-vesicles in preparation of antitumor drugs
The invention discloses application of camellia nitidissima exosome-like nano-vesicles in preparation of antitumor drugs, and belongs to the technical field of biological medicines. The exosome-like nano-vesicles are extracted from golden camellia for the first time, and the exosome-like nano-vesicles are proved to have remarkable anti-tumor activity.
Owner:SHENZHEN UNIV
Polygonum multiflorum exosome-like vesicle and application thereof
The invention relates to polygonum multiflorum exosome-like vesicles and application thereof, the polygonum multiflorum exosome-like vesicles are applied to preparation of antioxidant products, anti-inflammatory products and anti-ultraviolet products, the antioxidant products are products for removing one or more of DPPH free radicals, ABTS free radicals and hydroxyl free radicals, the anti-inflammatory products are products for inhibiting hyaluronidase, and the anti-ultraviolet products are products for removing one or more of DPPH free radicals, ABTS free radicals and hydroxyl free radicals. And the anti-ultraviolet product is a product capable of absorbing UVB. Compared with the prior art, the polygonum multiflorum exosome-like vesicles prepared by the method disclosed by the invention are low in cost and large in treatment capacity, and the obtained polygonum multiflorum exosome-like vesicles have excellent oxidation resistance, inflammation resistance and ultraviolet resistance.
Owner:SHANGHAI INST OF TECH
Method for extracting extracellular vesicles from dried traditional Chinese medicine product
The embodiment of the invention discloses a method for extracting extracellular vesicles from a traditional Chinese medicine dry product. The method comprises the following steps: taking a dry traditional Chinese medicine product as a raw material, and sequentially performing crushing, soaking, first-order enzymolysis, wall breaking, differential centrifugation, second-order enzymolysis, high-speed centrifugation, ultracentrifugation and ultrafiltration enrichment to obtain the extracellular vesicles. A two-step enzymolysis method is adopted in the enzymolysis process, impurities are removed to the maximum extent in a sequence difference enzymolysis mode, and the structure and function stability of the exosome are protected. According to the invention, the differential centrifugation and the enzymolysis method are alternately used, so that impurities with different sizes and different properties can be removed more thoroughly. According to the method, the cost is strictly controlled, the exosome purification efficiency is relatively high, the concentration of the obtained dry exosome is relatively high, and the operability is extremely high.
Owner:CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
Composition for promoting hair regeneration after chemotherapy as well as preparation method and application of composition
The invention provides a composition for promoting hair regeneration after chemotherapy as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: modifying the surface of a gold nanocage with polydopamine, loading the modified gold nanocage on an exosome, complexing magnesium ions and zinc ions to prepare a modified exosome, preparing a nano drug-loaded liposome from curcumin, resveratrol and epigallocatechin gallate, mixing the nano drug-loaded liposome with the modified exosome to prepare a multi-layer liposome, and preparing the multi-layer liposome from the nano drug-loaded liposome. And mixing with a penetration enhancer, and adding into a temperature-sensitive hydrogel system to prepare the composition for promoting hair regeneration after chemotherapy. The composition for promoting hair regeneration after chemotherapy has a good transdermal absorption promoting effect, can slowly release drugs, promotes hair follicles to be converted from a resting stage to a growing stage under the synergistic effect of the drugs, regulates and controls multiple pathways (such as anti-apoptosis, angiogenesis promotion and anti-inflammation) at the same time, is matched with a complex pathological mechanism of hair regeneration after chemotherapy, and can promote hair regeneration after chemotherapy. Wide application prospects are realized.
Owner:YANG SERIES (SHANDONG) BIOTECHNOLOGY CO LTD
Application of umbilical cord mesenchymal stem cell exosome in preparation of medicine for treating male infertility caused by hidden testis
PendingCN120549976ACell dissociation methodsSkeletal/connective tissue cellsSeminiferous tubuleMouse Testicle
The invention relates to the technical field of biological medicines, in particular to an umbilical cord mesenchymal stem cell exosome and application of the umbilical cord mesenchymal stem cell exosome in preparation of a medicine for treating male infertility caused by hidden testis. The prepared high-concentration umbilical cord mesenchymal stem cell exosome suspension preparation is directly used for treating cultured spermatogonial cells and spermatoblasts in vitro, is injected into the testis of a mouse, and is used for improving the seminiferous tubule and spermatogenic microenvironment of testis tissue and promoting division and proliferation of the spermatogonial cells and the spermatoblasts. The method can effectively reverse oligospermia and azoospermia caused by the hidden testis, and compared with a model without intervention of treatment means, the method can effectively promote increase and even regeneration of sperms in the testis.
Owner:FUJIAN MATERNAL & CHILD HEALTH HOSPITAL
Preparation method and application of exosome vesicles capable of efficiently loading drugs
The invention relates to the technical field of exosome loaded drugs, and provides a preparation method and application of exosome vesicles capable of efficiently loading drugs, and the preparation method comprises the following steps: exosome preparation: preparing an exosome sample with enough granularity and purity; pretreatment of exosomes: performing precipitation treatment and collecting the exosomes to prepare exosome precipitates; carrying out loaded medicine pretreatment: treating loaded medicine or a medicine preparation by using a lipid reagent; target drug loading: rapidly treating the exosome precipitate by using a drug lipid component, and then treating by using a buffer system to obtain the exosome load of the drug; and free lipid and drug removal: reducing free lipid and drug residues by re-enriching the exosomes. The problems of high concentration, high purity and low cost in industrial production of the exosome are solved, meanwhile, an efficient and convenient technical method is provided for taking the exosome loaded medicine as a delivery system, and the method can be suitable for the fields of health medical treatment, medical beauty cosmetics and biological medicine products.
Owner:BAIYANHUI (SHENZHEN) DOCTOR GRP CO LTD
Application and preparation method of exosome EGF-FGF-EXO containing double growth factors
The invention discloses application and a preparation method of exosome EGF-FGF-EXO containing double growth factors, the exosome EGF-FGF-EXO is uniform in particle size, high in loading efficiency and good in biological activity, the double growth factors comprise an epidermal growth factor EGF and a fibroblast growth factor FGF, and the epidermal growth factor EGF and the fibroblast growth factor FGF are added into the exosome EGF-FGF. The preparation method of the exosome EGF-FGF-EXO comprises preparation of an induced pluripotent stem cell (iPSC) exosome and loading of EGF-FGF, a recombinant epidermal growth factor (EGF) and a fibroblast growth factor (FGF) are loaded on the exosome from iPS-clone 12c, and the exosome product which has high biological activity and is used for treating nervous system diseases of neuroinflammation is prepared. The invention develops a brand new technical path for treatment of nervous system diseases, and in the fields of epilepsy, neurodegenerative diseases, brain injury and the like, the life quality of patients can be improved and important theoretical basis and technical support can be provided for precise medical treatment of nervous system diseases by precisely regulating and controlling neuroinflammation.
Owner:SUZHOU EV MEDICAL CO LTD
Preparation method of anti-aging cosmetic containing exosome
ActiveCN120284836ACosmetic preparationsToilet preparationsCentella asiatica extractRHODIOLA ROSEA ROOT
The invention discloses a preparation method of an anti-aging cosmetic containing exosome, and relates to the technical field of cosmetic preparation, the cosmetic is prepared from the following components in percentage by mass: 1.01%-5.05% of exosome wrapped by liposome, 0.5%-3% of an antioxidant, 1%-3% of a centella asiatica extract, 1%-3% of a rhodiola rosea extract, 10%-15% of a humectant and the balance of a basic matrix; wherein the exosome wrapped by the liposome is a mixture formed by mixing an exosome secreted by adipose-derived mesenchymal stem cells and an exosome secreted by plant cells according to a mass ratio of 1.5: 1.2; the plant is ginseng. According to the skin care product, the antioxidant, the centella asiatica extract and the exosome act together to improve the DPPH clearance rate of the skin care product, the skin brightness is improved, the roughness is reduced, and the excellent anti-aging performance is achieved. The lipid exosome-antioxidant-centella asiatica ternary system disclosed by the invention has a multi-target synergistic effect.
Owner:CARSON (JILIN) CELL RESOURCE LIBRARY MANAGEMENT CO LTD
Application of enhanced and optimized mesenchymal stem cell exosome in knee osteoarthritis and muscle cartilage injury
ActiveCN120346234AOrganic active ingredientsCell dissociation methodsCartilage injuryMesenchymal stem cell
The invention provides application of an enhanced and optimized mesenchymal stem cell exosome in knee osteoarthritis and muscle cartilage injury, and belongs to the technical field of biological medicine. The treatment activity of the umbilical cord mesenchymal stem cell exosome is remarkably improved by combining a composite pretreatment technology with a 3D culture system. Through the synergistic effect of the total flavonoids of rhizoma drynariae and tanshinone IIA, the pathological process of knee osteoarthritis can be regulated and controlled in multiple dimensions, including inflammatory reaction inhibition, cartilage repair promotion and joint function improvement, and the functional limitation of single-component pretreatment is broken through. The invention provides a safe and efficient novel treatment technical strategy for relieving knee osteoarthritis and muscle cartilage injury, and has a wide clinical application prospect.
Owner:WEST CHINA HOSPITAL SICHUAN UNIV +1
Method for efficiently separating and purifying plant exosome and application
The invention discloses a method for efficiently separating and purifying plant exosomes and application, and belongs to the technical field of biological nanotechnology. The method comprises the following key steps: (1) performing sterile cleaning on plant raw materials, and then performing peeling or tissue slitting pretreatment; (2) carrying out mechanical crushing treatment by adopting a phosphate buffer solution (PBS), and filtering through a 200-mesh screen to obtain a primary extracting solution; (3) performing gradient centrifugal purification on the extracting solution, collecting supernate, and filtering the supernate through a 0.45 mu m microporous filter membrane; (4) adding 8-12% of polyethylene glycol for low-temperature phase separation and precipitation; (5) purifying by using an electrodialysis technology; and (6) carrying out final concentration by adopting a 100kDa ultrafiltration membrane to obtain the high-purity PELNs preparation. According to the method, the process, equipment and reagents are simple and easy to obtain, the final yield is high, the purity is high, particle size distribution is uniform, and an innovative solution is provided for large-scale industrial production of natural-source nano-drug carriers.
Owner:ZHEJIANG UNIV OF TECH
Bionic exosome for anti-inflammatory and repair as well as preparation method and application of bionic exosome
The invention relates to a bionic exosome for anti-inflammation and repair as well as a preparation method and application thereof, and belongs to the field of cosmetics. The bionic exosome provided by the invention comprises a membrane structure and an internal load phase, the membrane structure comprises a phospholipid bilayer, a stabilizer and a targeting ligand; the internal load phase comprises a solvent and an active component; the phospholipid bilayer is composed of phospholipid compounds; preferably, at least one of the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and the 1, 2-dioleoyl-sn-glycerol-3-phosphoethanolamine is adopted as the phospholipid compound, and at least one of the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and the 1, 2-dioleoyl-sn-glycerol-3-phosphoethanolamine is adopted as the phospholipid compound; at least one of cholesterol, phytosphingosine and sodium stearoyl glutamate is preferably adopted as a stabilizer, so that the stabilizer of the obtained bionic exosome is improved, and the bionic exosome has the advantages of high encapsulation efficiency, good encapsulation efficiency stability, simplicity in operation, excellent anti-inflammatory and repairing effects and the like.
Owner:GUANGZHOU FANZHIRONG COSMETICS CO LTD +1
Polygonatum sibiricum-wolfberry heterozygous exosome as well as preparation method and application thereof
The invention relates to the field of biological medicine, and discloses a rhizoma polygonati-fructus lycii heterozygous exosome, a preparation method and application thereof and an antioxidant composition. The hybrid exosome comprises miRNA-156a (micro Ribonucleic Acid) and miRNA-396b (micro Ribonucleic Acid), and the expression quantity ratio of the miRNA-156a to the miRNA-396b is (1.2 to 1.8): 1; the heterozygous exosome is loaded with a polygonatum kingianum characteristic active component and a Chinese wolfberry fruit characteristic active component. The rhizoma polygonati-fructus lycii heterozygous exosome is good in biocompatibility and capable of remarkably improving the cell survival rate, and brand-new and efficient raw materials and technical supports are provided for development of anti-aging and anti-oxidation functional products.
Owner:HUNAN ACAD OF CHINESE MEDICINE
Exosome automatic identification and analysis method based on microscopic imaging
PendingCN120388373AAcquiring/recognising microscopic objectsNeural learning methodsMicroscopic imageFeature set
The invention provides an exosome automatic identification and analysis method based on microscopic imaging, and belongs to the technical field of exosome identification and analysis, and the method comprises the steps: collecting an exosome fluorescence microscopic image, carrying out the preprocessing, and obtaining an exosome region through adaptive threshold segmentation and morphological processing. A GPU is adopted to process and extract morphological, texture and statistical feature matrixes in parallel, and meanwhile, the CPU recognizes an abnormal region. And combining the features to form a region feature set, inputting the region feature set into the preliminary screening model for scoring and screening high-credibility regions. Deep feature extraction is carried out on the high-credibility region, and multiple features are fused by using an attention fusion mechanism to construct vectors; and inputting a depth classifier to obtain a classification probability matrix and a screening result. And finally outputting the number, position, classification and credibility score of the exosomes. The problems that an existing exosome analysis method often depends on manual recognition, and automatic and high-throughput analysis is difficult to achieve are solved.
Owner:QINGDAO RAISECARE BIOTECHNOLOGY CO LTD
Homogeneous electrochemical analysis method for exosome detection based on DNA-Pb < 2 + > functional nano hydrogel and application
The invention provides a homogeneous electrochemical analysis method for exosome detection based on DNA-Pb < 2 + > functional nano-hydrogel and application, and relates to the technical field of biomedical diagnostic analysis method.The homogeneous electrochemical analysis method is constructed based on DNA nano-hydrogel prepared through a rolling circle amplification (RCA) method by combining a G-quadruplex structure and a Pb < 2 + > specific recognition mechanism; an epithelial cell adhesion molecule (EpCAM) on the surface of the exosome is selected as a detection target, the DNA nano-hydrogel is triggered to disintegrate by utilizing specific binding between the EpCAM and an aptamer thereof, then a large number of G-quadruplex structures generated by RCA are exposed, the G-quadruplex structures can be selectively bound with Pb < 2 + > and regulate and control electrochemical signal output, and the detection sensitivity is high. According to the method, a one-pot method, a rapid and efficient detection process is realized, and the whole process is completed within 45 minutes.
Owner:CHENGDU MINSHAN CHUANGXIN BIOCHIP TECH CO LTD
Application of tea exosome in preparation of medicine for improving skin pigmentation
The invention relates to application of a tea exosome in preparation of a medicine for improving skin pigmentation, the tea exosome is creatively extracted and purified, and the tea exosome can effectively inhibit proliferation of melanoma cells and inhibit expression of melanin generation related protein by scavenging free radicals and inhibiting tyrosinase activity, so that the effect of improving skin pigmentation is achieved. The intracellular melanin removal is promoted. In a mouse in-vivo experiment, after the tea exosome is applied, it is observed that the skin epidermis thickness of a mouse is reduced, the dermis thickness is increased, collagen arrangement is tighter and more ordered, the content is increased, and meanwhile generation of inflammatory factors is reduced. The tea exosome can significantly increase the content of a melanin autophagy key factor LC3B and reduce the content of P62 to promote melanin autophagy, and has good percutaneous infiltration capacity, thereby playing a role in improving skin pigmentation.
Owner:FUJIAN MEDICAL UNIV UNION HOSPITAL
Lycium barbarum exosome as well as preparation method and application thereof
The invention relates to a method for preparing a Chinese wolfberry exosome, the Chinese wolfberry exosome prepared by the method and application of the Chinese wolfberry exosome. Specifically, the preparation method of the lycium barbarum plant exosome disclosed by the invention comprises a layer-by-layer filtration step, and the layer-by-layer filtration step sequentially comprises deep filtration and optional membrane filtration.
Owner:SHIBIMAN BIOTECHNOLOGY (SHANGHAI) CO LTD
Liquorice exosome-like extract as well as extraction and application thereof
The invention relates to the technical field of plant exosome-like extracts, in particular to a liquorice exosome-like extract as well as extraction and application thereof. The method comprises the following steps: firstly, removing impurities such as cells, cell debris, organelles, large vesicles and the like in homogenate by adopting a combination mode of low-speed centrifugation and medium-speed centrifugation, and further purifying the homogenate by adopting high-speed centrifugation after the low-speed centrifugation and the medium-speed centrifugation are completed, so as to obtain the exosome-like extract. And the setting of the increasing rotation speed is beneficial to finer separation of exosome-like particles with different sizes and densities, so that the purity and quality of the extract are improved. According to the method, all centrifugal operations are carried out in a low-temperature environment of 4 DEG C; the low-temperature condition is beneficial to maintaining the biological activity and stability of the sample, and the problems of protein denaturation, enzyme inactivation and the like caused by temperature rise are reduced, so that the quality and performance of the exosome-like extract are ensured.
Owner:SHANGHAI INST OF TECH
Coptis root exosome as well as extraction method and anti-tumor application thereof
The invention belongs to the technical field of preparation of anti-tumor drugs, and particularly relates to a coptis root exosome, an extraction method thereof and application of the coptis root exosome in tumor resistance. The coptis root exosome is extracted from coptis roots, the size of the coptis root exosome is 125nm-135nm, and the coptis root exosome is shaped like a vesicle with a lipid bilayer structure. The coptis root exosome has an excellent anti-tumor effect and can remarkably inhibit tumor growth, and the tumor growth inhibition effect is remarkably superior to that of a common extract berberine in coptis roots.
Owner:THE FIRST AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIVERSITY
Preparation method and application of sea cucumber exosome with immune cell repairing function
ActiveCN121628806ACosmetic preparationsCell dissociation methodsInflammatory factorsPharmaceutical drug
The invention discloses a preparation method and application of a sea cucumber exosome with an immune cell repairing function, and belongs to the technical field of biomedicine. The method comprises the following steps: feeding sea cucumbers by using a combined method of composite stress and an echinacea extract, extracting sea cucumber cells, and obtaining sea cucumber exosomes through ultracentrifugation; wherein the condition of composite stress is set as follows: the day and night temperature difference is not lower than 3 DEG C, and meanwhile, the dissolved oxygen content of the water body is adjusted to 75-90% of the dissolved oxygen content of the conventional feeding water body. The obtained sea cucumber exosome has a remarkable immune cell repairing function, and can effectively promote proliferation and activity recovery of immune cells, inhibit expression of inflammatory factors and improve the level of anti-inflammatory factors. The sea cucumber exosome prepared by the preparation method disclosed by the invention has an important application prospect in the research and development of immune repair drugs, and can provide a technical support for a novel immune regulation preparation in the field of biomedicine.
Owner:ZHONGPEPTIDE GUODA (BEIJING) BIOTECHNOLOGY CO LTD
Fruit and vegetable exosome extraction method based on ligand fishing
The invention discloses a fruit and vegetable exosome extraction method based on ligand fishing. The fruit and vegetable exosome extraction method comprises the following steps: suspending amino-functionalized Fe3O4 (at) SiO2 nanoparticles in a glutaraldehyde solution, and adding activated agglutinin to obtain Fe3O4 (at) SiO2-agglutinin nanoparticles; adding a glutaraldehyde solution into agarose gel to obtain a size exclusion chromatographic column filler; the method comprises the following steps: dispersing Fe3O4 (at) SiO2-lectin nanoparticles in a PBS buffer solution to obtain a uniform suspension, adding the uniform suspension into a size exclusion chromatographic column filler to obtain a uniform slurry, filling the size exclusion chromatographic column with the uniform slurry, and flushing with the buffer solution to form a magnetic bead stationary phase; and loading fruit and vegetable supernatant into a size exclusion chromatographic column, passing through the magnetic bead stationary phase, cleaning residual impurities combined on the exosome-magnetic bead compound by using a buffer solution, and dissociating the exosome from the magnetic beads by using a competitive eluent of the exosome to obtain the high-purity fruit and vegetable exosome. The problem that an existing exosome extraction method is difficult to meet the requirements of high efficiency, simplicity, high purity and high activity at the same time is solved.
Owner:SHAANXI UNIV OF SCI & TECH
Exosome RNA detection composition based on positive and negative targets and use method thereof
PendingCN121362841AMicrobiological testing/measurementDNA/RNA fragmentationReverse transcriptaseMicrobiology
The invention belongs to the field of biological detection, and discloses an exosome RNA detection composition based on positive and negative targets and a use method thereof, the composition comprises two positive targets and one negative target: the positive target A is miRNA stably expressed in exosome and is used for reflecting the expression level of small RNA; the positive target B is typical mRNA and is used for evaluating the quality of the long-fragment RNA; the negative target C is bacterial 16S rRNA and is used for identifying bacterial nucleic acid pollution in a sample. The method combines III-generation reverse transcriptase and HotStart Taq enzyme to construct a qPCR system, has high sensitivity and specificity, and is suitable for rapid detection of trace RNA. Whether the exosome RNA is suitable for downstream analysis or not can be judged by acquiring the Cq values of the three types of targets and setting a reference range. According to the invention, the integration of quality control, quantification and pollution identification of the exosome RNA is realized.
Owner:WISDELIVERY BIOTECHNOLOGY (WUHAN) CO LTD
Exosome compound preparation with hair growth effect and preparation method thereof
The invention relates to the technical field of exosomes, in particular to an exosome compound preparation with a hair growing effect and a preparation method of the exosome compound preparation. Comprising 12 to 22 parts of ginsenoside Rb1, 15 to 12 parts of biotin tripeptide-15, 8 to 15 parts of diammonium glycyrrhizinate, 50 to 70 parts of fish collagen peptide, 10 to 20 parts of soybean peptide, 0.5 to 4.5 parts of SHH protein, 0.5 to 2.5 parts of SMO inhibitor, 0.5 to 1.5 parts of HhSP protein, 5 to 10 parts of N-acetyl tyrosine, 0.1 to 2 parts of hyaluronic acid, 0.1 to 1 part of melatonin, 0.1 to 1 part of quercetin, 0.1 to 1 part of kaempferol, 0.1 to 1 part of tanshinone, 0.1 to 1 part of biotin and 0.1 to 1 part of vitamin A. The compound preparation is combined with exosomes from various sources, so that the hair papilla cell function is synergistically enhanced, and the damaged hair follicle structure is repaired.
Owner:SHANGHAI CHUNSHEN EXOSOME BIOMEDICINE CO LTD
Stimulation of angiogenesis by fibroblast derived exosomes
Disclosed are methods, means, and compositions of matter useful for the stimulation of angiogenesis directly by administration of membrane vesicles, such as fibroblast-derived exosomes, and / or through induction of angiogenic cytokines from blood cells contacted with fibroblast-derived exosomes. The invention provides means of treating conditions in which angiogenesis is beneficial through local or systemic administration of exosomes, including those derived from fibroblasts, wherein the fibroblasts are cultured under basal conditions or conditions of hypoxia. In other embodiments exosomes derived from fibroblasts are utilized to augment endogenous regenerative processes, such as hematopoiesis, angiogenesis and neurogenesis, as well as augment regenerative processes stimulated by administration of exogenous therapeutics such as cells, growth factors, or genes.
Owner:SPINALCYTE LLC
Exosome extraction device
PCT designated stage expiredWO2025152551A1Bioreactor/fermenter combinationsBiological substance pretreatmentsExosomeTube placement
An exosome extraction device, comprising: a base (4); a filter column placement disc (1), wherein the filter column placement disc (1) comprises a placement disc body (11), a plurality of extraction column placement holes (111) are formed in the placement disc body (11), the extraction column placement holes (111) are used for placement of exosome extraction columns (100), and the placement disc body (11) can be rotatably mounted on the base (4) relative to the base (4); an exosome collection disc (2), wherein the exosome collection disc (2) comprises a collection disc body (21), a plurality of collection tube placement holes (211) are formed in the collection disc body (21), the collection tube placement holes (211) are used for placement of exosome collection tubes (200), and the collection disc body (21) is rotatably mounted on the base (4) relative to the base (4); and a collection disc translation mechanism for driving the collection disc body (21) to move to a position below the placement disc body (11) or move away from the position below the placement disc body (11), wherein in the state where the collection disc body (21) is located below the collection disc body (11), the placement disc body (11) and the collection disc body (21) rotate, so that the collection tube placement holes (211) can be aligned with the extraction column placement holes (111) so as to collect exosomes dripping from the exosome extraction columns (100).
Owner:SHANGHAI UPPER BIO TECH PHARMA
Product capable of treating androgen alopecia and / or promoting hair growth and application of exosome
The invention discloses a product capable of treating androgenetic alopecia and / or promoting hair growth and application of exosomes, the product comprises the product capable of treating androgenetic alopecia and / or promoting hair growth, and is characterized by comprising the exosomes obtained by stimulating mesenchymal stem cells through CHIR99021 and minoxidil combined with the exosomes. The disclosure verifies that the exosome obtained by stimulating mesenchymal stem cells through CHIR99021 can promote hair growth and treat androgenetic alopecia, and particularly, the hair growth effect is better when the exosome is combined with minoxidil, so that a product comprising the exosome and minoxidil can treat androgenetic alopecia and promote hair growth is provided.
Owner:SHANDONG UNIV QILU HOSPITAL