11 results about "Serum insulin" patented technology

The invention provides an exosome, a mesenchymal stem cell and application of the exosome and the mesenchymal stem cell in treatment of type 2 diabetes mellitus, and belongs to the technical field of biological medicine. The exosome secreted by the umbilical cord mesenchymal stem cells of the composite pretreatment group is used for treating the type 2 diabetes mellitus, and the obvious advantages are shown. Compared with a model control group, the exosome has the advantages that the glucose tolerance of diabetic rats treated by the exosome is obviously improved, the blood glucose value is reduced by about 47.5% within 120 minutes, the area under a blood glucose curve (AUC) is reduced by about 50.5%, the serum insulin is increased by about 147.7%, insulin secretion is effectively promoted, the glycosylated hemoglobin level is obviously reduced, and the long-term blood glucose control effect is good. By integrating various indexes, the exosome obtained by the composite pretreatment scheme provided by the invention has a remarkable effect of improving the illness state of rats with type 2 diabetes mellitus, provides a new effective strategy for treatment of type 2 diabetes mellitus, and shows a good application prospect.

The invention provides a fructus cannabis protein peptide capable of reducing blood sugar and regulating glycometabolism and a preparation method and application thereof, and belongs to the technical field of functional active peptides, and the preparation method comprises the following steps: performing low-temperature supersonic fluid crushing on fructus cannabis, performing enzymolysis pretreatment on the crushed fructus cannabis, adding compound protease for enzymolysis, adding PNGaseF enzyme for further enzymolysis, and performing freeze drying to obtain the fructus cannabis protein peptide capable of reducing blood sugar and regulating glycometabolism. And carrying out membrane ultrafiltration interception and enrichment on the enzymolysis supernatant, carrying out tangential flow filtration, concentrating, drying and sterilizing to obtain the fructus cannabis protein peptide. Tests prove that the novel fructus cannabis protein peptide has the following remarkable effects that the fasting blood glucose level can be remarkably reduced, and uncomfortable symptoms such as thirst, polydipsia, diuresis and weakness caused by hyperglycemia are relieved; the symptoms of glucose intolerance and insulin resistance caused by hyperglycemia can be obviously relieved, and the onset risk of type 2 diabetes mellitus is reduced; the level of fasting serum insulin and glycosylated hemoglobin can be obviously reduced, and the level of serum GLP-1 can be improved.

The present invention discloses Parabacteroides distasonis NSP007 for alleviating insulin resistance and its application, belonging to the field of microbial technology. Parabacteroides distasonis NSP007 of the present invention can inhibit the weight gain and body fat accumulation of IR mice without affecting food intake; improve the glucose tolerance and insulin sensitivity of IR mice, while reducing the serum insulin and insulin resistance index of mice; regulate the lipid metabolism disorder of IR mice and relieve liver tissue damage; improve the intestinal barrier function of IR mice. The Parabacteroides distasonis NSP007 of the present invention is used for preparing a pharmaceutical composition for alleviating IR, and has very broad application prospects.

This invention discloses the application of a high-N-acetylglycine-producing *Lactobacillus reuteri* strain in alleviating obesity-related metabolic diseases, belonging to the field of microbial technology. The *Lactobacillus reuteri* NO1 strain of this invention (…) Limosilactobacillus reuteri (NO1) possesses the ability to produce N-acetylglycine in vitro. This strain can inhibit weight gain in obese mice without affecting food intake; improve glucose tolerance in obese mice while reducing blood glucose, serum insulin, and insulin resistance index; regulate liver triglycerides in obese mice, and alleviate liver tissue damage. The *Lactobacillus reuteri* strain described in this invention has very broad application prospects for preparing anti-obesity pharmaceutical compositions and fermented foods.

The application discloses a preparation method of a human insulin purity standard substance, which comprises the following steps of raw material selection, qualitative identification, standard substance uniformity, stability test, value determination and uncertainty evaluation. The application uses commercially available high-purity human insulin as a raw material, and after purity analysis, qualitative identification, uniformity test and stability test, isotope-labeled Val, Leu and Phe are used as internal standards, and liquid chromatography-isotope dilution mass spectrometry is used for value determination, so that the interference and influence of possible isoleucine substitution leucine impurities, isomer impurities or other molecular weight protein impurities on the value determination result can be effectively excluded, and the value determination result is more accurate and reliable. The purity standard substance prepared by the application can be used for serum insulin standard substance quality value tracing, quality control of insulin measurement results in in-vitro diagnosis, related method confirmation and evaluation, and evaluation of repeatability, linearity and cross contamination rate of a closed light-emitting immune analyzer.

A baked dough food for consumption during fasting, methods of making the same, and methods of fasting that include consumption of the baked dough food are disclosed herein. The baked dough food includes a dry ingredient selected from the group consisting of almond flour, coconut flour, Psyllium husk powder, chia powder, flaxseed powder, and combinations thereof, baking soda, at least one of clarified butter, an oil, cacao butter, almond butter, peanut butter, unsweetened almond milk, or water, a sweetener, and a flavoring agent. The baked dough food does not produce an insulin spike or increased blood glucose level upon consumption.

The invention relates to the technical field of external treatment of traditional Chinese medicine, and discloses an acupoint ironing moxibustion patch for treating children's dwarf disease in combination with a compound clove appetizing patch, the acupoint ironing moxibustion patch comprises: a non-woven fabric layer, which is used for providing a supporting effect and preventing the non-woven fabric layer from generating a hook line with clothes during use; the automatic heat production layer is arranged above the non-woven fabric layer and is used for providing a temperature rising capability; the medicine is arranged above the automatic heat production layer, ironing moxibustion is formed on the basis of heat energy generated by the automatic heat production layer, and the medicine is absorbed by a user when making contact with the skin of the Shenque acupoint and the body'zhu 'acupoint of the patient. Through cooperation of medicines and the automatic heat production layer, transdermal absorption of medicine components can be promoted, effects are achieved by stimulating acupuncture points, the heating temperature is controlled through the automatic heat production layer to conduct ironing moxibustion on the Shenque acupoint, the body'zhu 'acupoint and the like, the appetite, sleep and serum insulin-like growth factor-I of a patient are improved, and normal growth of the patient is recovered.

The present invention provides a hemp seed protein peptide for reducing blood sugar and regulating glucose metabolism, its preparation method and application, belonging to the technical field of functional active peptides, and comprising the following steps: The hemp seeds are subjected to low-temperature supersonic fluid pulverization, the pulverized hemp seeds are subjected to enzymatic pretreatment, compound protease is added for enzymatic hydrolysis, PNGaseF enzyme is added for further enzymatic hydrolysis to obtain an enzymatic hydrolysate, the supernatant of the enzymatic hydrolysis is enriched by membrane ultrafiltration interception, and then concentrated, dried and sterilized by tangential flow filtration to obtain the hemp seed protein peptide. The new hemp seed protein peptide in the present invention has been experimentally proven to have the following remarkable effects: It can significantly reduce the fasting blood sugar level and relieve the discomfort symptoms such as thirst, polydipsia, polyuria and fatigue caused by hyperglycemia; It can significantly relieve the symptoms of glucose intolerance and insulin resistance caused by hyperglycemia and reduce the risk of type 2 diabetes; It can significantly reduce the levels of fasting serum insulin and glycated hemoglobin and increase the level of serum GLP-1.

The invention discloses high-quality highland barley bread with a low glycemic index as well as a preparation method and application of the high-quality highland barley bread. The invention provides highland barley composite flour. The highland barley composite flour comprises black highland barley flour, vital gluten, wheat flour and rye flour. The invention also provides highland barley bread which is prepared by taking the highland barley composite powder as a main raw material and utilizing an enzyme preparation and an intermediate fermentation process. The addition amount of the black highland barley powder in the highland barley composite powder reaches 60%, postprandial blood sugar can be effectively controlled, the glycemic index is 49.7, the specific volume is 4.03 mL / g, and the hardness is 605 g. Experiments prove that the low-GI bread can reduce the fasting blood glucose by 46.3% and the serum insulin by 33.0%, and is beneficial to maintaining the blood glucose level. The highland barley bread provided by the invention is helpful for expanding the consumption market of bread, and meets the requirements of people on diversified food consumption and nutrition and health.

A baked dough food for consumption during fasting, methods of making the same, and methods of fasting that include consumption of the baked dough food are disclosed herein. The baked dough food includes a dry ingredient selected from the group consisting of almond flour, coconut flour, Psyllium husk powder, chia powder, flaxseed powder, and combinations thereof, baking soda, at least one of clarified butter, an oil, cacao butter, almond butter, peanut butter, unsweetened almond milk, or water, a sweetener, and a flavoring agent. The baked dough food does not produce an insulin spike or increased blood glucose level upon consumption.

The invention provides a method for evaluating the health of sows in the perinatal period based on metagenome sequences, a synthetic microbial community and application, and relates to the technical field of animal nutrition and biologication.The method comprises the steps of sample collection, index detection and judgment, microbial correlation analysis, screening platform construction and nutrient screening. According to the method, the problems that traditional diagnosis depends on highly invasive vein blood sampling and feedback lags behind are solved by excavating the relation between pregnant sow excrement microorganisms and serum insulin resistance and inflammatory factors. And early and accurate early warning of the metabolism health state of the sow can be realized by using fecal microorganisms. The constructed SynCom synthetic flora is not a general formula, but is a metabolic synergistic bacterium which is accurately locked based on big data and has the abundance significantly reduced in the perinatal period. Through directional supplement of the deleted members, excessive expansion of proinflammatory flora such as Prevotelasp.and the like can be effectively antagonized, and insulin resistance induced by inflammation is relieved from the source.