11 results about "Polydipsia" patented technology

The invention belongs to the technical field of polypeptide preparation and biological medicine, and particularly relates to a preparation method of Jingzhaotoxin polypeptide IX (JZTX-IX) and application of Jingzhaotoxin polypeptide IX in treatment of diabetes mellitus. A chilobrachys jingzhao toxin polypeptide IX coding gene, a 6 * His tag nucleotide sequence, a thrombin restriction enzyme cutting site nucleotide sequence and an MBP hydrotropy tag nucleotide sequence are connected to a prokaryotic expression vector for prokaryotic expression, and the expressed polypeptide is purified to obtain the chilobrachys jingzhao toxin polypeptide IX protein. The target polypeptide with high purity, complete disulfide bond folding and uniform conformation is obtained, so that the high purity and biological activity of the target polypeptide are ensured. On the basis, the treatment activity of the JZTX-IX on diabetes mellitus especially type II diabetes mellitus is found and verified, and the JZTX-IX can remarkably reduce fasting blood glucose of mice with the type II diabetes mellitus, improve glucose tolerance and insulin sensitivity, relieve polydipsia and polyuria symptoms and promote insulin secretion and islet cell proliferation.

The invention belongs to the technical field of polypeptide preparation and biological medicine, and particularly relates to a preparation method of Jingzhaotoxin polypeptide XI (JZTX-XI) and application of Jingzhaotoxin polypeptide XI (JZTX-XI) in treatment of diabetes mellitus. A Jingzhaotoxin polypeptide XI coding gene, a nucleotide sequence of a 6 * His tag, a nucleotide sequence of a thrombin restriction enzyme cutting site and a nucleotide sequence of an MBP hydrotropy tag are connected to a prokaryotic expression vector for prokaryotic expression, and a target polypeptide which is high in purity, complete in disulfide bond folding and uniform in conformation is obtained through purification. The purity and the biological activity are relatively high. Meanwhile, the treatment activity of the JZTX-XI in the aspect of diabetes is verified, and it is found that the JZTX-XI can remarkably reduce fasting blood glucose of mice with type II diabetes, remarkably up-regulate a glucose transport regulation gene Glut2, improve glucose tolerance and insulin sensitivity, relieve polydipsia and polyuria symptoms and promote insulin secretion and islet cell proliferation.

This invention discloses a medical repair hydrogel and its preparation method, specifically relating to the field of biomedical engineering technology. The hydrogel is a three-dimensional network structure formed by the synergistic self-assembly of glycyrrhizic acid and polydipsia glycoside through non-covalent bonds such as hydrogen bonding, hydrophobic interactions, and π-π stacking. No chemical cross-linking agent is required. It spontaneously forms a hydrogel upon heating to 70°C until completely dissolved and then cooling. It possesses reactive oxygen species-responsive structural dissociation characteristics, mechanical properties adapted to myocardial tissue, and a controllable degradation rate. This invention is suitable for intramyocardial drug administration after PCI, avoiding the shortcomings of traditional synthetic hydrogels that require external triggering and have unsuitable gelation conditions. Simultaneously, its three-dimensional network structure enables simultaneous long-term sustained release of the two active ingredients, with a 24-hour burst release rate of less than 25% and a cumulative release rate exceeding 70% within 7 days. This solves the problem of short half-lives and rapid clearance by body fluids of small molecule drugs, achieving continuous drug delivery to the lesion site.

The invention discloses a slurry traditional Chinese medicine formula and a preparation method thereof, and relates to the technical field of traditional Chinese medicine formulas. The pulp traditional Chinese medicine formula comprises the following raw materials in parts by weight: 75-85 parts of watermelon pulp, 10-15 parts of mulberries, 2.5-3.0 parts of dried lily bulbs, 4-6 parts of pears and 2.5-3.0 parts of glutinous rice; , 2.0 to 3.0 parts of dried chrysanthemum, 1.5 to 2.5 parts of fried cassia seed, and 1.0 to 1.5 parts of licorice. In the formula provided by the invention, the watermelon, the pear and the mulberry have the effects of clearing heat and promoting salivation, the lily and the glutinous rice have the effects of nourishing yin and moistening dryness, the chrysanthemum and the cassia seed have the effects of clearing liver and improving vision, and the liquorice is used for harmonizing the medicines. The medicinal and edible materials are used together to achieve the effects of nourishing yin and clearing heat, helping produce saliva and slake thirst, and improving eyesight and moistening dryness, and treat both symptoms and root causes aiming at the core pathogenesis of internal heat due to yin deficiency; the traditional Chinese medicine composition has a remarkable improvement effect on diuresis and polydipsia symptoms of diabetes insipidus, lacrimal insufficiency of dry eyes and initial hypertension. And the formula has no toxic or side effect, is sour, sweet and palatable in taste and good in patient compliance, and can be taken as a therapeutic drug or a daily health care product for a long time.

The present application provides a traditional Chinese medicine composition with blood sugar lowering efficacy and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines; the traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: birch juice 140-200 parts, jujube fruit 20-40 parts, rehmannia 20-40 parts, adenosma 18-26 parts, periploca 18-26 parts, ophiopogon 18-26 parts, bamboo shavings 12-19 parts, leech 12-19 parts, psyllium 12-19 parts, dogwood 10-14 parts, gordon euryale seed 10-14 parts. In the traditional Chinese medicine composition, the blood sugar lowering effect is mainly achieved by repairing the function of islet beta cells, promoting the regeneration of islet beta cells, improving sugar metabolism, increasing the sensitivity of insulin or improving insulin resistance, so that the overall prescription achieves the effects of invigorating the spleen and lung, nourishing yin and reducing fire, benefiting the stomach and producing saliva, astringing the spleen essence, promoting transportation and transformation, tonifying the kidney and strengthening the body, and relieving polydipsia and restlessness.

The present application relates to a kind of traditional Chinese medicine composition of invigorating spleen and dampness and its preparation method and application, including the following weight parts of raw materials: 7-14 parts of ginseng, 10-20 parts of fried white bean, 10-20 parts of tuckahoe, 7-14 parts of orange peel, 15-30 parts of corn silk, 10-20 parts of gardenia, 12-24 parts of white lily root, 13-26 parts of kudzu root, 6-12 parts of white peony root, 6-12 parts of perilla leaf, 6-12 parts of shiitake mushroom, 1-4 parts of licorice root. It has the effect of invigorating spleen and dampness, ascending clear and descending turbid, promoting water and reducing swelling. It is used for body fatigue, limb soreness, edema, vomiting, diarrhea, polydipsia, loose stool and other symptoms caused by dampness. And it can be used as a health drink for chronic diseases related to dampness. The present application has a good application prospect with unique formula and no toxic side effects.

The invention discloses a construction method and application of a polycystic kidney disease mouse model. The invention provides a method for constructing a polycystic kidney mouse model, which comprises the following steps: inhibiting or reducing the expression quantity of a Talpid3 gene or / and the activity of a Talpid3 protein in renal tubular epithelial cells of a mouse to obtain a renal tubular epithelial cell specific Talpid3 gene knockout mouse; the polycystic kidney mouse model is obtained. According to the invention, a Talpid3 knockout mouse is constructed and cultivated, the Talpid3 knockout mouse is obviously reduced in body size and lighter in weight, the ratio of kidney to body weight is obviously increased, serious renal cyst appears along with serious renal dysfunction, urine concentration function decline and polydipsia and diuresis pathological manifestations, and the Talpid3 knockout mouse is successively dead 8 weeks after birth, so that the Talpid3 knockout mouse has no toxic or side effect. The typical symptoms of the polycystic kidney disease are shown, and the polycystic kidney disease mouse model is established.

Disclosed is an application of glycerol monodecanoate (GMD) in the preparation of a hypoglycemic drug or a functional food, falling within the technical field of medicine. According to the present disclosure, the hypoglycemic and insulin resistance-improving functions of GMD are first revealed. Studies demonstrate that GMD can enhance glucagon-like peptide-1 (GLP-1) secretion, activate hepatic glycolysis and insulin receptor (INSR)-related gene expression, while inhibiting hepatic gluconeogenesis pathway-related gene expression. Notably in type 2 diabetes mellitus (T2DM), glucose-lowering efficacy of GMD is comparable to metformin, and it outperforms metformin in regulating glucose tolerance and insulin tolerance, with significant alleviation of polydipsia. Moreover, as GMD is a naturally occurring edible substance with a high safety profile, it has a good application prospect in the preparation of a drug for treating hyperglycemia and diabetes, or a functional food to assist in lowering blood glucose.

The invention discloses a traditional Chinese medicine prescription for early treatment of diabetes and diabetic nephropathy and a preparation method thereof. The traditional Chinese medicine prescription is based on rhizoma atractylodis, radix scrophulariae and herba eupatorii decoction and comprises the following raw materials in parts by weight: 10-20g of rhizoma atractylodis, 10-20g of radix scrophulariae, 10-20g of herba lycopi and 10-20g of herba The traditional Chinese medicine prescription is used together to form a three-in-one treatment system of tonifying spleen and drying dampness, promoting blood circulation to remove meridian obstruction and inducing diuresis to alleviate edema, and has a remarkable curative effect on early symptoms of diabetes and diabetic nephropathy, such as thirst, liking to drink, weakness, lassitude, edema, bitter and sticky mouth, sticky and unsmooth excrement, foam urine, deep and thin pulse or fine and astringent pulse, fat and crack tongue, thick and greasy tongue coating and the like.

The invention discloses a medicine for dispelling effects of alcohol and protecting liver and a preparation method thereof. The medicine is prepared from kudzuvine root, hovenia dulcis thunb, orange peel, fructus alpiniae oxyphyllae, raw malt and rosa roxburghii tratt. The traditional Chinese medicine has the effects of eliminating alcohol toxicity, tonifying spleen and removing food retention, protecting liver and kidney, relieving restlessness and arresting vomiting, and inducing astringency and stopping diarrhea; the wine accumulation syndrome is mainly treated. The wine is suitable for the symptoms such as excessive drinking, drunkenness, vomiting and acid regurgitation, chest and diaphragm distension and stuffiness, dizziness and headache, polydipsia, disadvantageous urination and defecation and the like, and can also be used for preventing drunkenness.

The present application relates to the technical field of physiological measurement, in particular to a kind of diabetes symptom automatic detection system, system includes: blood glucose fluctuation analysis module, polydipsia and polyuria symptom detection module, recession level evaluation module, progress stage identification module.In the present application, through the correlation analysis of real-time blood glucose monitoring and patient diet state, the change of blood glucose level is accurately captured, the development trend of disease is predicted, the real-time of disease monitoring is enhanced, the medical intervention is more timely and effective, through the continuous monitoring of the amount of water and urine of patient, the symptoms of polydipsia and polyuria of diabetic patients are found in time, and the correlation between target symptom and diabetes progression is determined through data analysis, the identification ability of early diabetic complications is enhanced, combined with the evaluation of organ function level, the signs of organ damage are identified, help to adjust treatment strategy, prevent the development of complications, improve the real-time and accuracy of disease monitoring, reduce the complexity and cost of long-term treatment.