125 results about "Disulfide bonding" patented technology

The application discloses a photosensitizer conjugate for targeted treatment of tongue squamous cell carcinoma and a preparation method and application thereof, and belongs to the technical field of biological medicines. A novel PROTAC-PDT conjugate Gef-PRO-SS-TPE is synthesized, and the compound connects a PROTAC molecule Gef-PRO targeting EGFR and an AIE photosensitizer TPE derivative through a breakable disulfide bond. On one hand, Gef-PRO can specifically degrade EGFR and block tumor cell proliferation signals; on the other hand, the TPE derivative can generate ROS under light conditions and induce cell apoptosis. More importantly, the disulfide bond can be broken in response to high concentrations of glutathione (GSH) in tumor cells, realizing controllable release of Gef-PRO and TPE molecules, so as to exert a synergistic anti-tumor effect.

This invention discloses a weather-resistant, high-hardness composite anti-corrosion coating and its preparation method, belonging to the field of anti-corrosion coating technology. The preparation method includes: firstly, synthesizing an epoxy resin containing dynamic disulfide bonds by reacting dithiodibenzoic acid and epichlorohydrin in an alkaline aqueous phase; secondly, constructing a low surface energy siloxane network using hydroxyl-terminated polydimethylsiloxane and methyltriethoxysilane; subsequently, introducing chlorzoxazone and N-acetyl-p-aminophenol as composite organic corrosion inhibitors, blending them with the resin to obtain a composite base material; finally, adding graphene, carbon nanotubes, and inorganic anti-corrosion fillers, and cross-linking and curing to form a film using a polyamine curing agent. This invention suppresses microcracks through the dynamic stress relief of disulfide bonds, combined with the weather-resistant barrier of the siloxane network, the active interface passivation of the heteroatom corrosion inhibitor, and the three-dimensional synergistic mechanical bonding of carbon-based nanomaterials, achieving a perfect unity of ultra-strong corrosion resistance, excellent weather resistance, and high mechanical hardness in the coating.

This invention discloses a class of thiadiazole-benzohexacyclic compounds, their preparation methods, and applications. The structural formula is shown in Formula I. This invention introduces disulfide bond fragments into the thiadiazole-benzohexacyclic skeleton to obtain a novel class of thiadiazole-benzohexacyclic compounds. These compounds have a novel structure, a simple preparation method, readily available raw materials, and are unlikely to produce harmful byproducts. The compounds exhibit inhibitory effects against various plant pathogenic bacteria, particularly against *Citrus canker*, *Ralstonia solanacearum*, and *Bacterial angular leaf spot* of cucumber, demonstrating significant inhibitory effects. They can be used as agricultural bactericides and have great application value.

This invention discloses a hair repair composition for permed hair, comprising, by weight: 0.02-1.0 parts hydrolyzed plant protein, 0.1-2.0 parts biomimetic amino acid complex, 50-95 parts carrier, and 0.5-15 parts additives. The hydrolyzed plant protein is obtained from Moringa seed protein through multi-enzyme synergistic hydrolysis, with a molecular weight less than 3500 Daltons. The biomimetic amino acid complex includes at least wheat amino acids, soybean amino acids, arginine hydrochloride, serine, and threonine. Under weakly acidic conditions, this composition can simultaneously repair broken disulfide bonds, hydrogen bonds, and ionic bonds in hair. The hydrolyzed plant protein provides disulfide bond reconnection sites, and the biomimetic amino acid complex constructs a multi-point weak bond network through hydroxyl and ionic groups, achieving "triple bond repair" synergistically. This invention can significantly improve the mechanical strength, elasticity, and breakage resistance of damaged hair, with long-lasting, gentle, and safe repair effects, suitable for daily care of damaged hair after perming and dyeing.

The application discloses a toughened composite polyurethane impact-resistant material based on dynamic covalent bond energy regulation, and belongs to the technical field of polyurethane materials. A double dynamic covalent network containing DA bonds and disulfide bonds is constructed by reacting polyurethane prepolymer, furfurylamine, cystine diethyl ester and bismaleimide, then nano molybdenum disulfide is modified by silane, then a metal-polyphenol / polymer composite interface layer is constructed on the surface of aramid fiber by tannic acid-iron complex and polyethyleneimine, finally, the components are mixed, impregnated and hot-pressed to obtain the composite polyurethane material. The application effectively dissipates impact energy through the reversible rupture and recombination of dynamic covalent bonds, and enhances the interfacial bonding force between components by using multiple interface modification, so that the material has excellent impact resistance, high strength, high ductility and efficient self-repairing function, solves the problems of easy damage of the interface of traditional composite materials, insufficient impact resistance and irreversible damage, and has wide application prospect in the fields of safety protection and buffer components.

The application discloses a kind of polypeptides based on limulus and its application of antifungal selective modification.The amino acid sequence is as shown in SEQ ID No:1-22,wherein the polypeptide cyclic peptide of the amino acid sequence shown in SEQ ID No:2 forms disulfide bond between 3C and 12C, and the polypeptide of the amino acid sequence shown in SEQ ID No:1-22 is linear peptide.The polypeptide antibacterial agent SEQ ID NO:1-11 of the application minimizes the impact on bacteria while maintaining potent inhibition of fungi, which is conducive to maintaining the balance of microbial community and exhibits great application potential in medical and personal care products.

The preparation and application of spaced double disulfide bond bridged dimer prodrug and self-assembled nanoparticles thereof belong to the technical field of medicine, and relate to the synthesis of the connection of the spaced double disulfide bond bridged dimer prodrug shown in structural general formula (I) and the construction of the spaced double disulfide bond bridged dimer prodrug shown in structural general formula (II) and self-assembled nanoparticles thereof, and the application thereof in drug delivery. The preparation method is simple and easy to implement, the spaced double disulfide bond bridged dimer prodrug can be self-assembled to form nanoparticles, and has the ability of redox dual response drug release, so that the intelligent response activation of the prodrug in tumor cells can be realized, and the anti-tumor effect and safety of the prodrug are ensured. The anti-tumor effect, pharmacokinetic parameters and safety thereof are superior to those of the prodrug nanoparticles bridged by a single disulfide bond. The present application provides a new strategy for developing a high-efficiency low-toxicity drug delivery system, and meets the urgent needs of high-end chemotherapy preparations in clinical practice.

The present inventors succeeded in improving the antibody constant region to have increased stability under acid conditions, reduced heterogeneity originated from disulfide bonds in the hinge region, reduced heterogeneity originated from the H chain C terminus, and increased stability at high concentrations as well as in discovering novel constant region sequences having reduced Fcγ receptor-binding, while minimizing the generation of novel T-cell epitope peptides. As a result, the present inventors successfully discovered antibody constant regions with improved physicochemical properties (stability and homogeneity), immunogenicity, safety, and pharmacokinetics.

The application relates to the technical field of biological medicine, and discloses a targeted ferroptosis-inducing conjugate as well as a preparation method and application thereof. The structural formula of the conjugate is as follows: the conjugate contains a prostate cancer targeting peptide segment WHDGFK, and is connected with an iron death-inducing antibacterial peptide KRIVKWIIKLLR through a disulfide bond, so that the conjugate has tumor targeting and microenvironment response release functions, not only endows the antibacterial peptide KRIVKWIIKLLR with tumor selective delivery capacity, but also realizes specific release of the active peptide in target cells by reducing the disulfide bond by using high-concentration glutathione (GSH) in tumor cells, thereby synergistically enhancing the antitumor effect and reducing the systemic toxicity.

This invention discloses a bio-based elastomer, belonging to the field of new materials technology. The low-temperature self-healing bio-based polyurethane elastomer of this invention consists of soft segments and hard segments. The soft segments are composed of hydroxyl-terminated aliphatic prepolymers and semi-crystalline polymer polycaprolactone diol, while the hard segments include isocyanates and chain extenders. A bio-based polyester prepolymer with an amorphous structure and compliant long chains is prepared by multi-component copolymerization. Lowering its glass transition temperature yields a prepolymer with low-temperature resistance potential. Using the semi-crystalline polycaprolactone diol as part of the soft segments, a dynamic layered domain can be constructed through a crystal-locked dynamic network, serving as a rigid filler to reinforce the elastomer. Simultaneously, a rich network of hydrogen bonds, urethane esters, and disulfide bonds is introduced into the elastomer, endowing it with low-temperature self-healing properties, thus producing a polyester-type low-temperature self-healing bio-based elastomer constructed using a multi-dynamic network.

The application belongs to the technical field of polymer self-repairing elastomer materials, and specifically discloses an alkali-resistant self-repairing polyurethane hybrid elastomer and a preparation method and application thereof. The elastomer realizes the synergistic improvement of mechanical properties, self-repairing capability and environmental tolerance by synergistically introducing dynamic disulfide bonds, hydrophobic siloxane segments and rigid aromatic crosslinking structures in the same polyurethane network. The preparation method uses polyurethane precursors as raw materials, dissolves the precursors in N,N-dimethylformamide (DMF), and then casts into a film and dries, so that the process flow is simple and easy to operate. The surface scratch of the elastomer can be closed and repaired within 120 min at 60 DEG C, and the tensile property remains stable after multiple damage-repair cycles. Meanwhile, the hydrophobic siloxane-rich phase acts as a micro diffusion barrier, so that the material can still maintain the stability of chemical structure and mechanical properties under the condition of soaking in strong alkaline medium with pH = 12.

The application discloses a kind of ultrafast metal-free and solvent-free preparation method for preparing symmetric and asymmetric disulfide, which comprises the following steps: at room temperature, raw material R-SH, R'-SH and oxidant and base are added to reaction bottle, shake rapidly for 10-30 seconds, and R-S-S-R' is prepared after the reaction product is purified;Wherein R in R-SH is aliphatic or aromatic group, R' in R'-SH is aliphatic or aromatic group;R in R-SH and R' in R'-SH are same or different;The aromatic group has substituent or no substituent, and the aliphatic is straight chain or has branch;The oxidant is chloroform, and the base is N, N-diisopropylethylamine (DIPEA).The method is simple in operation, high in yield (60-99%), ultrafast in reaction kinetics, greatly improves production efficiency, wide in applicability, avoids the use of organic reaction solvent, raw material is cheap and easy to obtain, and does not contain residual metal and other sticky contaminants from reagent and ligand, provides a safer and more effective method for the synthesis and production of symmetric and asymmetric disulfide-containing compounds.

The application belongs to the technical field of bioinformatics, and discloses a protein disulfide bond mutation site prediction method and device, equipment and a storage medium. The predicted structure of a to-be-transformed protein is acquired to extract a plurality of candidate residue pairs, and the structural feature information of each candidate residue pair is extracted, including the inter-residue distance matrix of the two residues, the sequence distance, and the relative solvent accessible surface area, depth index and predicted local distance difference test value of each residue. The predicted model is input to obtain the probability value of each candidate residue pair forming a disulfide bond. The candidate residue pair with a probability greater than a specified probability is determined as a disulfide bond mutation site. Therefore, more comprehensive features can be used to comprehensively predict the bonding probability of the disulfide bond, the model is less dependent on individual traditional features, the high sensitivity of the traditional method to atomic coordinates and the uncertainty of the prediction result are overcome to a certain extent, the robustness is higher, and the prediction accuracy of the protein disulfide bond mutation site is improved.

The application discloses a methionine adenosyltransferase mutant and application of the mutant in immobilized reaction, the mutant is obtained by mutating an amino acid sequence of wild-type Escherichia coli methionine adenosyltransferase, the mutant enhances the interaction force between subunits by introducing a disulfide bond at a specific position; and / or, the protein stability is enhanced by constructing a connecting peptide between adjacent subunits. The mutant provided in the application enhances the thermal stability and catalytic efficiency of the enzyme, reduces the optimum reaction pH value, reduces the generation of by-products and the precipitation of a reaction solution. The mutant enzyme can maintain high activity at a temperature of about 40 DEG C, and is particularly suitable for industrial production. In addition, the immobilized form of the mutant enzyme exhibits good stability and reusability in continuous use, significantly reduces production cost, simultaneously reduces environmental pollution, improves the yield of SAM, is more suitable for large-scale industrial production, and has more market competitiveness.

This invention discloses a conductive lignin-based polyurethane film, its preparation method, and its applications. The preparation method includes: using lignin sulfonate from papermaking waste liquid as raw material, extracting lignin sulfonic acid from it, and efficiently converting it into lignin sulfonic acid polyol under mild conditions; bridging the flexible chain of polyester diol with isocyanate; and introducing supramolecular interaction units containing dynamic disulfide bonds and hydrogen bonds into the LPU matrix to construct a dynamic covalent crosslinking network. Then, conductive materials are added to the film liquid and uniformly mixed, cast to a mold, and cured to form a film, thus obtaining the conductive lignin-based polyurethane film. The preparation method provided by this invention uses inexpensive and readily available raw materials, has high modification efficiency, a simple preparation process, and generates no waste, making it environmentally friendly. The obtained conductive lignin-based polyurethane film can be applied to wearable flexible sensors, exhibiting excellent mechanical properties, heat resistance, conductivity stability, UV shielding, reprocessability, and low cost.

The present application relates to the field of lithium ion battery silicon negative electrode material. The existing lipoic acid biological binder depends on water phase coordination, and is easy to fail in organic electrolyte. The polysulfide lipoic acid homopolymer has too strong flexibility, lacks polar functional groups, has low bonding strength, and cannot stabilize the electron / ion channel. The present application provides a kind of dynamic self-repairing polymer binder, which is prepared by polymerization reaction of a multivariate copolymer containing vinyl unsaturated carboxylic acid monomer, unsaturated amide monomer and sulfur-containing compound containing cyclic disulfide structure. The binder uses dynamic disulfide bond response volume change stress, dissipates stress and repairs microcracks through reversible fracture / recombination; the polar group forms multiple interactions with active material and current collector, enhancing the interface adhesion; the dynamic coordination characteristics optimize ion transmission. Experimental results show that the electrode exhibits excellent cycle stability, mechanical integrity and rate performance, effectively solving the failure problem caused by volume expansion of silicon negative electrode.

A method for producing and recycling a crosslinked photopolymer is disclosed. The method includes mixing reactive thiols, multifunctional alkenes, and a photoinitiator to create a homogeneous mixture that is crosslinked through exposure to light. The method also includes decrosslinking the crosslinked photopolymer through base-catalyzed thiol-disulfide exchange reactions by mixing the crosslinked photopolymer with a reactive thiol, a base catalyst, and a solvent to create a decrosslinked material including recycled thiol oligomers which are reactive. The method further includes removing the base catalyst and the solvent and recrosslinking the recycled thiol oligomers by mixing stoichiometric amounts of the recycled thiol oligomers and a reactive alkene such that a molar ratio between thiol end groups and ene end groups is maintained at 1:1. The method includes adding a photoinitiator and mixing to create a homogeneous mixture, and recrosslinking the homogeneous mixture through exposure to light. The recrosslinked photopolymer includes dynamic disulfide bonds.

Presented herein is a recombinant OPCML-Fc fusion protein molecule and methods of its use in cancer treatment where the Fc portion of the fusion protein molecule is only the CH2CH3 portion and lacks the hinge region containing the disulfide bridges preventing dimerization of the Fc portion and optimizing dimerization of the OPCML the D1 domain.

The application provides a preparation method of water-promoted self-healing modifier modified asphalt, and specifically comprises the following steps: first, polyol is dehydrated, and then polymerized with diisocyanate to obtain a prepolymer; the prepolymer is uniformly mixed with a disulfide chain extender, a gel monomer, a crosslinking agent, an initiator, a promoter and a solvent to obtain a reaction copolymer; the copolymer is heated and solidified; the solidified copolymer is uniformly mixed with a base asphalt to obtain a reaction mixture; and the reaction mixture is heated and solidified to obtain the modified asphalt with the water-promoted self-healing modifier. The application aims to provide a kind of modified asphalt which synchronously polymerizes polyurethane with dynamic covalent bond and hydrogel with high water absorption, and is used as an asphalt modifier, and prepares modified asphalt with water-promoted self-healing. The hydrogel with water absorption is introduced into the polyurethane system, through the dynamic exchange of disulfide bond and the water absorption performance of the hydrogel, so that the modified asphalt has excellent water-promoted self-healing performance.

This invention relates to the field of polypeptide drug synthesis technology and discloses a method for synthesizing etakatide. The synthesis method of this invention simplifies the reaction process by combining the deprotection step of D-Cys with thiol activation, making the operation simple and inhibiting the formation of dimer impurities. It also avoids sulfonation impurities caused by disulfide bond construction in the liquid phase, thereby improving the yield and reducing production costs. This method can be applied to the production of etakatide.

This invention relates to the field of polymer composite materials technology, and provides a polymer wear-resistant material and its preparation method. The material is made from the following raw materials: epoxy resin, ZIF-67@NiCo wear-resistant reinforcement containing coordination unsaturated sites, mussel-inspired multifunctional interface bridging agent containing dynamic covalent bonds, amino-terminated polydimethylsiloxane, curing agent, and additives. This invention constructs a closed-loop intelligent response system of friction triggering, in-situ catalysis, interface self-reinforcement, and lubrication self-replenishment: ZIF-67@NiCo functions as both a wear-resistant filler and an in-situ catalyst; the interface bridging agent contains dynamic disulfide bonds, and bond regeneration during crack propagation achieves interface self-repair; the amino-terminated PDMS re-bonds with the bridging agent under ZIF catalysis to achieve lubrication self-replenishment; the synergistic effect of these three components results in a coating adhesion ≥17MPa, a friction coefficient ≤0.12, wear amount ≤15mg / 1000r, and self-repair efficiency ≥80%, extending the coating life. The preparation process of this invention is simple, suitable for industrial production, and can be widely used in the inner surface protective layer of industrial equipment.

The present application relates to a kind of high temperature steaming-room temperature stacking-physical scale removal wool shrink-proof finishing method based on wide temperature range protease, belong to wool fabric dyeing and finishing field.The present application is impregnated with reducing agent-protease composite working solution to fabric, and working solution includes reducing agent, wide temperature range protease, penetrant and strong strength protective agent and other components;After impregnation, high temperature steaming process is carried out, the disulfide bond in wool scale layer and lipid structure are destroyed, and the hydrolysis efficiency of protease to scale layer is improved;After repeated impregnation working solution, room temperature stacking process is carried out, realize the sufficient hydrolysis, stripping and falling of protease to wool scale under limited condition, finally through physical scale removal process, strengthen shrink-proof effect, so that the shrinkage of the prepared wool fabric reaches machine washable standard (shrinkage <6%), and the strength loss rate is lower (<12%).

The application discloses a fermentation method for improving structural stability and yield of recombinant human serum albumin, and belongs to the technical field of biological fermentation. 600 ≥180, and the living cell rate is greater than or equal to 90%; (2) during a methanol induction period, L-cysteine and L-methionine are added in batches in pulse mode with a mass ratio (0.5-2.0):1, and the final concentration is 0.1%-0.3%. The application promotes correct formation of disulfide bonds by optimizing seed quality and protein folding microenvironment. By using the method, the expression amount of rHSA reaches 24.3 g / L, the monomer purity is greater than or equal to 92%, the free sulfhydryl content is less than or equal to 3%, and the monomer purity reduction rate is less than or equal to 4% after 14 days of accelerated storage at 37 DEG C. The application significantly improves product stability while maintaining high yield, and provides an innovative process for industrialized production of high-quality rHSA.

Disclosed herein are synthetic VP1 proteins that contain engineered disulfide bonds. In some embodiments, the synthetic VP1 proteins with engineered disulfide bonds self-assemble into non-infectious virus-like particles (VLPs) that are more stable that non-infectious VLPs that resulted from self-assembly of VP1 proteins that lack engineered disulfide bonds.

This invention discloses a dynamically cross-linked, wide-temperature-range environmentally friendly damping composite material and its preparation method, belonging to the technical field of damping composite materials. The key technical point is that the damping composite material comprises the following raw materials in parts by weight: 50-80 parts butyl rubber, 20-50 parts polymethyl methacrylate, 10-40 parts hindered phenol, 3-8 parts disulfide bond extender, 5-15 parts phenolic resin vulcanizing agent, 10-20 parts modified mica powder, 5-20 parts plasticizer, and 3-5 parts zinc oxide. The weight ratio of butyl rubber to polymethyl methacrylate is (5-8):(2-5). A blend system of butyl rubber and polymethyl methacrylate is used. By adjusting the amount of hindered phenol and introducing a disulfide bond extender, the microphase separation structure is optimized to form a bicontinuous phase, allowing the damping peaks to superimpose and merge, thus widening the damping temperature range to -50~105℃, covering the entire operating condition from cold start to high temperature, while also effectively reducing the migration and precipitation of hindered phenol.

This invention relates to the field of organic materials technology, specifically to a high-strength, on-demand debonding UV-curable adhesive, its preparation method, and its applications. The adhesive is prepared using raw materials including: disulfide-bonded polyurethane acrylate oligomers, disulfide acrylic acid reactive monomers, acrylic diluents, photoinitiators, and coupling agents. This invention employs a two-step method to synthesize the disulfide-bonded polyurethane acrylate oligomers. The oligomers are mixed with photoinitiators, disulfide acrylic acid molecules, reactive diluents, and silane coupling agents in a specific ratio. After vacuum degassing in the dark, the mixture is coated onto the surface of the bonded parts and cured by UV irradiation for 1 minute. This method features mild reaction conditions, a simple preparation process, high raw material conversion rate, and high product purity, effectively solving the problem of controllable debonding in existing UV-curable adhesives. It is suitable for temporary fixation of precision devices and material recycling applications.

This invention discloses a chemiluminescent immunoassay method, comprising the following steps: labeling disulfide-bonded biotin or desulfobiotin onto affinity ligand one to form complex one; labeling a luminescent compound onto affinity ligand two to form complex two; mixing the sample with a solid-phase carrier containing complex one, complex two, and streptavidin-coupled compound to form an immunosandwich complex; processing the immunosandwich complex to release the immunosandwich complex from the solid-phase carrier; separating the immunosandwich complex from the solid-phase carrier; detecting the total luminescent signal value of the separated immunosandwich complex; and calculating the concentration of the analyte based on the total luminescent signal value. This invention releases the immunosandwich complex from the solid-phase carrier, uses a magnetic field to attract the solid-phase carrier, and measures the supernatant of the released immunosandwich complex. Solid-phase carriers containing non-specific adsorption cannot enter the measurement chamber, completely eliminating the problem of non-specific adsorption on the surface of magnetic beads.

This invention provides a cell culture additive composition for preventing antibody disulfide bond reduction and its application, relating to the biopharmaceutical field. It comprises the following raw materials: a redox regulator, a metal ion chelator, an antioxidant, an adjuvant, and a cell protectant. This invention inhibits antibody disulfide bond reduction from multiple aspects through the synergistic effect of the redox regulator, metal ion chelator, antioxidant, and buffer. Specifically, the redox regulator maintains a suitable redox potential, the metal ion chelator removes harmful metal ions, the antioxidant removes free radicals, and the buffer maintains pH stability. The components work together synergistically to enhance the effect.

This invention discloses a polyurethane adhesive, its preparation method, and its applications, belonging to the field of secondary battery technology. It addresses the problems of existing adhesives' inability to adaptively repair electrode microcracks and their difficulty in long-term binding of highly expandable active materials, leading to short battery cycle life. The polyurethane adhesive of this application comprises aliphatic polymeric polyol, polyisocyanate, and chain extender in a molar ratio of 1:(1.5~2.5):(0.5~1.5); the chain extender includes aliphatic amino acid esters containing disulfide bonds or aliphatic polyols containing disulfide bonds. When the polyurethane adhesive of this invention is applied to electrodes containing highly expandable active materials, it can achieve long-term binding, thereby improving the cycle life of high-capacity batteries.