1173 results about "Disulfide bonding" patented technology

This disclosure relates to dimeric immunotherapeutics that comprise two IgGs that are crosslinked with a disulfide bond. The two IgGs may be chimeras of two different heavy chains, in which one heavy chain includes a cysteine mutation that forms the disulfide bond, and the other heavy chain lacks the cysteine mutation. The presence of a cysteine mutation in only one of the heavy chains of an IgG avoids two disulfide bonds between the two IgGs, which increases the accessible orientations between the two crosslinked IgGs, and also avoids the formation of trimers and higher-order oligomers.

The invention provides a self-repairing polyurethane film containing a disulfide bond and a DA bond and a preparation method thereof, and the preparation method comprises the following steps: (1) adding an organic solvent into a three-necked flask, then dissolving dihydric alcohol, diisocyanate, a disulfide bond monomer and a DA bond forming monomer in the organic solvent, then adding a catalyst, and mechanically stirring uniformly; (2) introducing nitrogen into the three-necked flask to evacuate air, setting a reaction temperature, and carrying out a reaction under mechanical stirring; (3) in a nitrogen environment, dropwise adding a monomer II forming a DA bond into the solution obtained after the reaction in the step (2), and after the dropwise addition of the monomer II forming the DA bond is completed, setting the reaction temperature and reacting for a period of time; and (4) pouring the solution obtained after the reaction in the step (3) into a polytetrafluoroethylene mold, and drying to obtain the self-repairing polyurethane film. The self-repairing polyurethane film prepared by the preparation method disclosed by the invention has relatively good mechanical properties, relatively good self-repairing performance under mild conditions and high self-repairing efficiency.

The invention discloses silk fibroin composite gel and a preparation method thereof, and relates to the technical field of tissue filling materials. The preparation method of the silk fibroin composite gel comprises the following steps: preparing silk fibroin and carrying out amination modification on the silk fibroin to obtain aminated silk fibroin; carrying out dynamic cross-linking on the aminated silk fibroin and the oxidized sodium alginate through a Schiff base bond by adopting an enzyme catalysis cross-linking process to obtain a dynamic cross-linked network solution; adding a temperature-sensitive component into the dynamic cross-linked network solution, and uniformly mixing to obtain a temperature-sensitive-dynamic dual-network pre-gel solution with temperature responsiveness; loading the active component into the temperature-sensitive-dynamic dual-network pregel solution to obtain a composite gel solution; incubating the composite gel solution to obtain primary gel; after disulfide bonds are introduced into the primary gel, cross-linking strengthening and sterilization are carried out, and the silk fibroin composite gel is obtained. Through temperature-sensitive-dynamic dual-network design, active components and disulfide bonds are introduced to strengthen crosslinking, and the problems that traditional injectable gel is irreversible, single in function, prone to collapse after being filled and the like are systematically solved.

The invention relates to the technical field of automobile tires, and discloses a new energy automobile tire self-repairing sealing material adhered with mute cotton. The preparation method comprises the following steps: adding a modified polyurethane adhesive, C5 petroleum resin and a modified carbon nanotube filler into absolute ethyl alcohol, mixing to obtain a self-repairing liquid, adhering polyurethane cotton to the upper part of the self-repairing liquid, and drying to obtain the new energy automobile tire self-repairing sealing material adhered with the mute cotton. Disulfide bonds, boric acid ester bonds and imine bonds introduced into polyurethane through a chain extension reaction and diselenide bonds introduced into carbon nanotubes all belong to dynamic covalent bonds, so that the self-repairing performance of the material is improved; by introducing a rigid benzene ring and naphthyl aromatic structure, the mechanical property of the self-repairing sealing material is improved; the carbon nano tube can play a role in skeleton supporting in a polyurethane matrix, and the mechanical property of the material is further improved by introducing an adamantane rigid structure into the carbon nano tube.

The invention relates to a polypeptide ligand and a derivative of E3 ubiquitin ligase as well as a preparation method and application of the polypeptide ligand and the derivative, and relates to the technical field of biological medicines. The polypeptide ligand of the E3 ubiquitin ligase is characterized in that the amino acid sequence of the polypeptide ligand is shown as any one of SEQ ID NO: 1-3, and at least one disulfide bond is formed between cysteine residues in the amino acid sequence of the polypeptide ligand; the polypeptide ligand is combined with the E3 ubiquitin ligase RNF43 and / or the E3 ubiquitin ligase ZNRF3. The polypeptide ligand provided by the invention can be used for flexibly and modularly constructing various bifunctional molecules, such as small-size bifunctional molecules or bifunctional molecules with components which cannot be obtained through expression, so as to degrade disease-related membrane proteins, such as PD-L1, EGFR and the like, and has the potential of disease treatment.

The invention belongs to the field of electrochemical energy, and relates to polyurethane with self-repairing capability, a solid electrolyte, a composite electrode and a preparation method, a soft segment part of the self-repairing polyurethane-based solid electrolyte has good segment motion capability, and compared with polyethylene glycol, the crystallinity is greatly reduced; and the ionic conductivity and the lithium ion transference number are greatly improved. The dynamic disulfide bond and the hydrogen bond of the hard segment part jointly form a dynamic reversible macromolecular network, so that the mechanical strength is considered, and the tensile property, the self-healing property and the repeatable processing property are good. The self-repairing polyurethane-based solid electrolyte has relatively high ionic conductivity and lithium ion transference number, and can be cured on an electrode in situ to eliminate the interface contact problem of the electrolyte and the electrode, and the positive electrode and the negative electrode of the battery are healed into a whole through the self-healing capability of polyurethane, so that the performance of the battery is greatly improved, and the service life of the battery is prolonged. And the material has a good application prospect in all-solid-state lithium batteries.

The invention relates to the technical field of films, in particular to a flexible film special for a handwriting board and a preparation method of the flexible film. The flexible film is obtained by coating a release film with coating slurry and carrying out thermocuring, wherein the coating slurry contains modified polyurethane, a flatting agent, a defoaming agent and ethyl acetate. According to the modified polyurethane, polycarbonate diol, isophorone diisocyanate and other raw materials are subjected to a reaction, and a nano particle chain extender containing disulfide bonds is added. The nano particle chain extender containing disulfide bonds is prepared by modifying nano silicon dioxide and grafting cystamine dihydrochloride and diamino polyethylene glycol. Through nanoparticle surface modification and dynamic disulfide bond network construction, molecular-level coupling of a rigid reinforced phase and a flexible substrate, inhibition of nanoparticle aggregation and balance of strength and toughness are realized, the dynamic disulfide bond network can self-repair microcracks, and a high-quality special flexible film solution is provided for a high-performance handwriting board.

The embodiment of the invention relates to the field of energy storage, and provides a secondary battery and a preparation method thereof, an energy storage system and electric equipment. The negative electrode active material comprises a silicon-based material, honeycomb porous graphite and a polypyrrole layer, the surface of the honeycomb porous graphite is coated with the polypyrrole layer, the silicon-based material is embedded in pores of the honeycomb porous graphite, and the silicon-based material comprises silicon particles, a poly N-isopropylacrylamide layer and a disulfide bond-containing polyaniline layer. The poly (N-isopropylacrylamide) layer and the disulfide bond-containing polyaniline layer are coated on the surfaces of the silicon particles, the poly (N-isopropylacrylamide) layer is positioned between the silicon particles and the disulfide bond-containing polyaniline layer, and the honeycomb porous graphite comprises a graphite flake and a carbon layer which is positioned on the surface of the graphite flake and is of a honeycomb pore structure. The secondary battery formed by assembling the negative electrode active material disclosed by the invention has relatively high cycling stability, high temperature resistance, rate capability, gram volume and safety.

The invention discloses two-dimensional porous nano NiFe2O4 and a preparation method of a self-repairing noise-reducing anticorrosive coating of the two-dimensional porous nano NiFe2O4. The NiFe2O4 precursor is prepared by adopting a hydrothermal synthesis method, the nano NiFe2O4 with a stable and regular two-dimensional porous structure is prepared by high-temperature calcination, and the nano NiFe2O4 is rich in pores and relatively large in specific surface area. Polyester glycol, diisocyanate and disulfide are used as raw materials, addition polymerization is performed according to the molar ratio of 1: 2: 1 to prepare a polyurethane matrix, and key process parameters such as a reaction solvent, atmosphere, temperature and time are optimized. Chemical bonds such as disulfide bonds are introduced, so that the coating is endowed with excellent self-repairing performance, and meanwhile, the noise reduction capability and the anti-corrosion effect of the coating are remarkably enhanced. The two-dimensional porous nano NiFe2O4 reinforced polyurethane prepared by the invention provides a brand new design thought and technical path for the field of self-repairing noise-reducing anticorrosive materials.

The invention discloses a cyclic peptide compound as well as a preparation method and application thereof, belongs to the field of polypeptide synthesis and application, and particularly relates to full-protection peptide resin prepared by solid-phase synthesis. Carrying out cutting and monocyclization on the full-protection peptide resin to obtain monocyclic peptide; the monocyclic peptide is subjected to binary cyclization treatment to obtain a cyclic peptide compound Cyclo (His-Lys-Cys-Gly-His-Lys-Cys-Gly-, and a disulfide bond is in bridge connection with Cysamp; and the binary cyclization is cyclized under the action of iodine methanol and ascorbic acid. The cyclopeptide compound prepared by the invention has the advantages of low toxicity, good stability, good antioxidant effect and good anti-inflammatory effect, and the expression of type I collagen can be improved. Therefore, the invention has the advantages of low toxicity, good stability, good antioxidant effect and good anti-inflammatory effect, and can improve the expression of the type I collagen.

The invention provides a preparation method of a self-repairing waterborne polyurethane coating based on a synergistic effect of a dynamic disulfide bond and a pH response microcapsule. The preparation method comprises the following steps: step 1, preparing a dynamic disulfide bond-containing waterborne polyurethane (D-WPU) emulsion; step 2, preparing a pH response microcapsule; step 3, preparing a self-repairing waterborne polyurethane coating; a dynamic disulfide bond of the self-repairing waterborne polyurethane coating rapidly repairs macrocracks at 50-60 DEG C through mercaptan catalysis, and pH < lt > is caused by local corrosion of a pH response microcapsule; when the pH value is 4.0, cysteine is released, sulfydryl accelerates recombination of dynamic bonds, and amino enhances cohesive force of the coating and neutralizes H < + >. And in combination with a hydrogen bond network of the nanocellulose, the coating has low-temperature repair (the mechanical strength recovery rate is greater than or equal to 90%), high toughness (the water absorption rate is less than or equal to 2.1%) and long-acting corrosion resistance (the salt spray resistance life is greater than or equal to 600 hours), and is suitable for severe environments such as ocean engineering and new energy equipment.

The invention discloses a light-moisture dual-curing underwater epoxy coating with a self-repairing function as well as a preparation method and a construction method of the light-moisture dual-curing underwater epoxy coating. The epoxy coating comprises a component A and a component B, wherein the mass ratio of the component A to the component B is (1.5-3.5): 1; the component A is prepared from the following raw materials: bisphenol F type epoxy resin, dynamic disulfide bond modified polysulfide rubber, boron nitride nanosheet and silicon carbide nanowire hybrid filler, a photoinitiator and a silane coupling agent; the component B is prepared from the following raw materials: moisture-cured polyether amine, a boric acid ester bond cross-linking agent and hydrophobic modified graphene oxide. According to the invention, a light-moisture dual-curing mechanism is adopted, ultraviolet irradiation initiates rapid surface curing, and then environmental moisture permeates to complete deep curing. The material provided by the invention has the advantages of rapid curing, excellent self-repairing function, significantly improved underwater adhesion and good thermal conductivity, and provides a new solution for rapid repairing and long-acting protection of underwater structures.

The invention discloses a self-repairing nylon elastomer material as well as a preparation method and application thereof. The preparation method comprises the following steps: S1, polymerizing a nylon monomer in the presence of an amine end-capping reagent to prepare a double-amino-terminated nylon hard segment; s2, adding the double-amino-terminated nylon hard segment, the double-carboxyl-terminated polyether and / or polyester soft segment and a disulfide bond-containing chain extender into a reactor, adjusting the proportion of the raw materials, and polymerizing under an optional auxiliary A to obtain a double-amino-terminated pre-polymerized nylon elastomer; and S3, adding the double-amino-terminated pre-polymerized nylon elastomer and a dialdehyde chain extender into the reactor, and carrying out a reaction in the presence of an optional auxiliary agent B to obtain the self-repairing nylon elastomer material. According to the self-repairing nylon elastomer material provided by the invention, the nylon elastomer can be self-repaired in various environments through the synergistic effect of various dynamic covalent bonds, and the polymerization efficiency is remarkably improved.

The invention relates to the technical field of bioactive peptides, in particular to an antioxidant peptide based on mussel byssus, a preparation method, a preparation and application of the antioxidant peptide based on mussel byssus, the antioxidant peptide is designed and synthesized by referring to the structural characteristics of mussel byssus proteins mfp-3, mfp-5 and mfp-6, the antioxidant peptide comprises four sequences of MFP3-AP1, MFP5-AP2, MFP6-AP3 and MFP-Hybrid, tyrosine in the peptide is modified into DOPA through enzymatic modification, and the antioxidant peptide is prepared through enzymatic modification. The stability and activity of the antioxidant peptide are enhanced through disulfide bond formation, metal ion coordination, PEGylation and other modifications, and the antioxidant peptide has high DPPH free radical scavenging activity, ABTS free radical scavenging activity, hydroxyl free radical scavenging activity and iron ion reducing capacity, has a protective effect on the cellular level, can be prepared into a liquid preparation, a freeze-dried preparation or a micro-capsule preparation, and can be used for preparing the antioxidant peptide. The compound is expected to be applied to the fields of antioxidant health care products, cosmetics and medicines.

The invention belongs to the technical field of ocean engineering materials, and particularly relates to an underwater concrete self-repairing material containing dynamic disulfide bonds as well as a preparation method and application of the underwater concrete self-repairing material. The underwater concrete self-repairing material containing the dynamic disulfide bond comprises the following components in parts by weight: 50-70 parts of a hardening agent which is an epoxy prepolymer containing the dynamic disulfide bond; the water repellent agent comprises 20-35 parts of a curing agent and 1-3 parts of an accelerant. According to the underwater concrete self-repairing material containing the dynamic disulfide bonds, the epoxy prepolymer containing the dynamic disulfide bonds serves as a main network, the epoxy prepolymer is broken under the action of external force, and then new covalent bonds can be formed through spontaneous recombination to repair microcracks.

The invention discloses a preparation method and application of a zinc-bromine flow battery positive electrode material, and belongs to the field of zinc-bromine flow batteries. The preparation method comprises the following steps: dissolving aniline and a dithio compound in an acidic ice bath, dropwise adding a peroxy strong oxidant to generate a polyaniline-disulfide bond copolymer, dialyzing, and freeze-drying to obtain powder; then heating soluble silver salt, a reducing agent, a hydrophilic dispersing agent and an organic sulfydryl silane coupling agent to prepare functionalized nano-silver particles; the powder and nano-silver particles are dispersed in N-methyl pyrrolidone for ultrasonic treatment, and dynamic self-repairing coating slurry is prepared; and performing acid pretreatment on the carbon fiber paper, and performing electrochemical deposition on the slurry to prepare the zinc-bromine battery positive electrode material. According to the zinc-bromine battery positive electrode material, the coulombic efficiency and the voltage efficiency of a zinc-bromine flow battery are remarkably improved and the cycle life of the zinc-bromine flow battery is remarkably prolonged through methods of dynamic covalent bonds, nano-silver catalysis, a hierarchical pore structure, carbon fiber pretreatment and the like, and the zinc-bromine flow battery positive electrode material has chemical stability, efficient bromine adsorption capacity and excellent catalytic activity.

The invention discloses a bidirectional reversible conversion method and system between a peptide molecule SMILES and a sequence expression. The core innovation lies in that a new sequence description syntax is defined to retain information of a polypeptide special bond and specific modification of amino acid; a main chain atom index and adjacency traversal topology identification algorithm is adopted, and end group and topology integrated detection and coding are carried out; a residue recognition algorithm for main chain cutting and template library matching is compatible with any standard or non-standard amino acid residues, an extensible end group library / monomer template library and an automatic increment mechanism, and automatic recognition and sequence annotation of S-S disulfide bonds; the invention relates to a high-fidelity assembly algorithm of HELM anchor points and topology aware cyclic peptide processing. The method solves the problems of incapability of supporting a complex polypeptide topological structure, poor reversibility, insufficient expansibility of a monomer library and the like in the prior art, can be widely applied to scenes of quantitative structure-activity relationship model construction, large-scale polypeptide data cleaning and the like, and has remarkable practicability and innovativeness.

The invention provides a lipid compound based on a lipoic acid derivative, a composition as well as a preparation method and application of the lipid compound. When the lipoic acid-derived lipid provided by the invention is used as a delivery carrier of a nucleic acid drug, because disulfide bonds in the compound are split under the action of glutathione in cells, the compound is degraded, so that the timely release of nucleic acid is triggered, the cytotoxicity caused by accumulation of the compound in the cells is well reduced, and the bioavailability of the nucleic acid drug is improved. The biocompatibility is good. When the lipid nanoparticle composition is used as a carrier to deliver nucleic acid drugs, the lipid nanoparticle composition has excellent delivery efficiency and excellent spleen targeting performance, and the nucleic acid drugs can be preferentially delivered to the spleen.

The invention relates to a structural design, synthesis and application of a biomass carbon dot modified poly L-cysteine-S-S-carboxylic acid amphiphilic polypeptide. The invention provides a structural design, synthesis and application of poly (L-cysteine-S-S-carboxylic acid) amphiphilic polypeptide (P-L-cys) modified by biomass carbon dots (CDs). Comprising synthesis of P-L-cys which contains hydrophobic groups, provides hydrophilic and functionalized carboxyl and provides reduction response by disulfide bonds, synthesis of CDs and CDs modified P-L-cys (CDs (at) P-L-cys), and the macroscopic performance change of the polypeptide is explored from regulation factors of a polypeptide secondary structure and a self-assembly nanostructure assembly mechanism. The P-L-cys and the CDs (at) P-L-cys prepared by the invention have functionalization, fluorescence imaging and dual-response drug release and have particle sizes suitable for drug delivery and enrichment, and the CDs also provide a photo-thermal and photodynamic synergistic treatment effect and have good application prospects in the fields of drug delivery, cancer resistance and the like.

The invention discloses a washable easily-colored wool fabric treatment process, belongs to the technical field of wool fabric processing, and aims to solve the technical problem that the dyeing degree, the anti-felting performance and the color fastness of wool fabrics in the prior art need to be further improved. The method includes the following steps that disulfide bonds of wool fibers are opened through L-cysteine, the washable and easy-to-color wool fabric is obtained, the pretreated wool fibers prepared through the method are grafted with methoxy polyethylene glycol methacrylate under catalysis of hydrogen peroxide and horse radish peroxidase, modified wool fibers are obtained, and the washable and easy-to-color wool fabric is obtained. After the modified wool fibers are woven into the wool fabric, the wool fabric is respectively subjected to padding modification by using the modified dye solution and the finishing liquid, so that the degree of dyeing, the anti-felting performance and the color fastness of the wool fabric are improved.

The invention provides a chemically modified tetanus toxin C fragment as well as preparation and application thereof. The method for chemically modifying the tetanus toxin C fragment comprises the following steps: 1) reacting the tetanus toxin C fragment with a reducing agent to reduce a disulfide bond in the tetanus toxin C fragment; and 2) carrying out a reaction on the reduced tetanus toxin C fragment and an alkylation reagent, and carrying out modification. The recombinant tetanus toxin C fragment prepared by the method disclosed by the invention not only meets the quality standard required by pharmacopeia, but also has the advantages of stable conformation, high yield, easiness in amplification and low cost, and has a wide application prospect in the preparation of human vaccines, veterinary vaccines and antibody drugs and the development process of in-vitro diagnostic reagents.

The invention belongs to the field of biological pharmacy, and relates to a temperature-sensitive hydrogel, in particular to a temperature-sensitive hydrogel SV (at) HP based on hyaluronic acid as well as a preparation method and application of the temperature-sensitive hydrogel SV (at) HP. The temperature-sensitive hydrogel SV (at) HP entraps CS-siRNA nanoparticles and VEGF (at) PLGA microspheres, and is used for realizing microenvironment responsive drug delivery to treat intrauterine adhesion. Chlorogenic acid and TGF beta1 siRNA are self-assembled through disulfide bonds to form CS-siRNA nano-particles, the CS-siRNA nano-particles are used for treating intrauterine adhesion through gene and chemical therapy, and cell and animal experiments prove that the CS-siRNA nano-particles have a good effect of improving intrauterine adhesion. Through evaluation of a real-time fluorescence quantification method, a western blot method, a cell scratch experiment, laser confocal microscope observation, inverted fluorescence microscope observation, an in-vivo experiment and the like, the SV-coated HP gel has the effects of resisting inflammation, improving endometrial fibrosis, promoting IUA rat endometrial angiogenesis and repair, inhibiting intrauterine adhesion and improving the intrauterine environment.

The present invention discloses a method for improving the properties of composite meat protein gel based on modified pea protein, which belongs to the technical field of food protein processing. The present invention fully dissolves natural pea protein or pH-shifted modified pea protein to obtain a protein solution; then adds a disulfide bond cleaving agent to the protein solution, dissolves and mixes the solution to obtain modified pea protein. The present invention is based on the disulfide bond cleavage treatment of pea protein to dissociate acidic and basic subunits and expose more internal groups, thereby achieving the improvement of the solubility and functionality of pea protein itself. In addition, both natural pea protein and modified pea protein with disulfide bond cleavage can enhance the structural properties of low-salt meat protein gel under the action of cross-linking enzymes, develop low-salt meat protein gel with good gelation and network structure, and provide solutions and technical support for the quality deterioration of meat products under various circumstances.

The invention discloses a leukemia targeted therapy preparation as well as a preparation method and application thereof. Comprising a DNA nanoflower carrier targeting leukemia cells through a PTK7 receptor and benzene arsenic oxide loaded on the carrier. The DNA nanoflower carrier is obtained by rolling circle amplification, and a nucleic acid aptamer complementary sequence of targeted binding leukemia cells is introduced into a template chain subjected to rolling circle amplification. The preparation method comprises the following steps: modifying sulfydryl onto a DNA nanoflower by using DNA-Dithiol, then breaking a disulfide bond by using reduced glutathione to expose the sulfydryl, and loading benzene arsenic oxide onto the DNA nanoflower through a reaction between trivalent arsenic and the sulfydryl. The structure of the DNA nanoflower has a high surface area and high porosity, and can load a large amount of PAO, so that the killing performance on cancer cells is enhanced; the aptamer sgc8 sequence is introduced into the DNA nanoflower, so that the targeting of the DNA nanoflower to cancer cells is effectively realized; the preparation also has the advantages of being good in thermodynamic stability, easy to store, excellent in biocompatibility and the like, and has a very good application prospect.

The invention discloses a protein-based adsorbent capable of efficiently adsorbing perfluorinated compounds and application of the protein-based adsorbent. The adsorbent is obtained by coating polyethyleneimine in a phase transition aggregate formed by unfolding protein through a disulfide bond reducing agent. The use method of the adsorbent comprises the step of feeding the adsorbent into a solution containing the perfluorinated compound to realize efficient adsorption of the perfluorinated compound. The adsorbent has the advantages of being low in cost, efficient, easy to operate, environmentally friendly, free of secondary pollution, good in biocompatibility and the like, efficient selective adsorption of perfluorinated compounds can be achieved through the adsorbent, the adsorbent can be repeatedly used, and industrial application and popularization are easy.

The invention discloses a nanorana alpine antioxidative peptide and application thereof. The antioxidative peptide is a cyclic peptide composed of 29 amino acids, the amino acid sequence of the antioxidative peptide is as shown in SEQ ID NO.1, and cysteine at the seventh position and cysteine at the twelfth position form an intramolecular disulfide bond. The invention further discloses nucleotide for coding the antioxidative peptide of the nanorana pleskei. The antioxidative peptide has good anti-inflammatory and anti-oxidation effects, can protect plasmid DNA damaged by H2O2, inhibits accumulation of CuSO4 induced zebrafish larva active oxygen and migration of neutrophil, and relieves mouse skin injury induced by UVB radiation.

The invention provides an ELISA (Enzyme Linked Immunosorbent Assay) kit for detecting an anti-MDA5 antibody and a preparation method of the ELISA kit. According to the kit, full-length human MDA5 protein (1025 amino acids) expressed by a cell-free protein expression system under the low-temperature condition of 22 DEG C is adopted as a coating antigen, and a disulfide bond enhancer E6820 is added into the system to promote correct folding of the protein. The kit comprises a microwell plate coated with a full-length MDA5 antigen, an enzyme-labeled anti-human IgG secondary antibody, a standard substance with the concentration range of 1-243 ng / mL, a chromogenic substrate and an optimized confining liquid system. The invention solves the problem of insufficient sensitivity caused by the use of fragment antigens in the prior art, and can be used for early diagnosis and illness monitoring of dermatomyositis-related interstitial lung diseases.

The invention belongs to the technical field of protein engineering modification, and particularly relates to a method for improving thermal stability of fumonisins degrading enzyme by forming disulfide bonds. The thermal stability of the fumonisins degrading enzyme FumDPS mutants T194V, T194V-P40C / Y173C, T194V-N43C / D83C and T194V-F71C / S117C related to the invention is obviously improved compared with that of the wild type fumonisins degrading enzyme, and the thermal stability of the fumonisins degrading enzyme FumDPS mutants is obviously improved. The fumonisins enzyme is mainly applied to the industries of livestock feed processing, food production and the like so as to reduce the harm of fumonisins to the health of animals and human beings. The obtained fumonisins degrading enzyme mutant with improved thermal stability is convenient to transport and store, so that the investment of industrial cost can be reduced.

The invention discloses lithium battery diaphragm coating slurry with a self-repairing function and a preparation method of the lithium battery diaphragm coating slurry, and belongs to the technical field of lithium battery diaphragm materials. In order to solve the problem of potential safety hazards caused by the fact that an existing lithium battery wet diaphragm is prone to generating small cracks, self-repairing waterborne polyurethane containing dynamic covalent bonds (such as disulfide bonds) is synthesized through molecular structure design, and the self-repairing waterborne polyurethane, ceramic particles, a binder and other components are dispersed to prepare coating slurry; and a self-repairing coating with the thickness of 5-10 microns is formed on the surface of the diaphragm through a roller coating or coating process. The coating is high in repairing efficiency, meanwhile, the tensile strength and ionic conductivity of the diaphragm are improved, the problem of mechanical damage caused by stress fatigue of the diaphragm is effectively solved, the safety of the lithium battery is remarkably improved, and the cycle life of the lithium battery is remarkably prolonged. The preparation method is simple in process and good in environmental protection property, and a brand new technical scheme is provided for preparation of the high-reliability lithium battery diaphragm.

The invention provides a pharmaceutical wastewater treatment process. The pharmaceutical wastewater treatment process comprises the following steps: A1, pretreatment, A2, adsorption treatment, A3, oxidation treatment and A4, filtration treatment. In the step A2, a composite adsorbent comprising the following raw materials: attapulgite, activated carbon, carbon nanotubes, 4-carboxyphenylboronic acid, polyethyleneimine, sodium dithiocarbamate, epoxy resin E-44, deionized water and modified nano zero-valent iron is used, and self-repairing is realized through dynamic chemical bonds such as boric acid ester bonds, imine bonds and thiol-disulfide bonds; the adsorbent can be self-repaired during microscopic damage, so that the service life is prolonged, and relatively high adsorption capacity is kept. In the step A3, a gel microsphere oxidant comprising the following raw materials: hydrogen peroxide, potassium persulfate, copper-iron sulfide, nitrogen-doped carbon quantum dots, sodium alginate powder, sodium polyacrylate and a phosphate buffer solution is used, and efficient degradation is realized through a synergistic mechanism of free radical oxidation, non-free radical oxidation and photocatalysis.