39 results about "Disulfide Linkage" patented technology

The instant invention describes methods of separating or preferentially synthesizing dimers which are linked via at least one interchain disulfide linkage from dimers which are not linked via at least one interchain disulfide linkage from a mixture comprising the two types of polypeptide dimers. These forms can be separated from each other using hydrophobic interaction chromatography. In addition, the invention pertains to connecting peptides that result in the preferential biosynthesis of dimers that are linked via at least one interchain disulfide linkage or that are not linked via at least one interchain disulfide linkage. The invention also pertains to compositions in which a majority of the dimers are linked via at least one interchain disulfide linkage or are not linked via at least one interchain disulfide linkage. The invention still further pertains to novel binding molecules, e.g., comprising connecting peptides of the invention.

The present invention relates to microparticles comprising a gel body, wherein the gel body comprises a synthetic polymer and a drug, wherein the microparticles have an average diameter in the range 40 to 1500 μm, wherein the polymer is cross-linked by groups comprising disulfide linkages and is in the form of a hydrogel.

The instant invention describes methods of separating or preferentially synthesizing dimers which are linked via at least one interchain disulfide linkage from dimers which are not linked via at least one interchain disulfide linkage from a mixture comprising the two types of polypeptide dimers. These forms can be separated from each other using hydrophobic interaction chromatography. In addition, the invention pertains to connecting peptides that result in the preferential biosynthesis of dimers that are linked via at least one interchain disulfide linkage or that are not linked via at least one interchain disulfide linkage. The invention also pertains to compositions in which a majority of the dimers are linked via at least one interchain disulfide linkage or are not linked via at least one interchain disulfide linkage. The invention still further pertains to novel binding molecules, e.g., comprising connecting peptides of the invention.

The instant invention describes novel multispecific binding molecules comprising synthetic connecting peptides. The synthetic connecting peptides result in the preferential synthesis of multispecific binding molecules comprising polypeptide chains that are linked via at least one interchain disulfide linkage. In addition, the invention pertains to compositions in which a majority of the multispecific binding molecules comprising polypeptide chain that are linked via at least one interchain disulfide linkage or are not linked via at least one intrachain disulfide linkage. In a specific embodiment, the invention pertains to compositions comprising multispecific dimeric binding molecules said molecules comprising at least a first binding site specific for a tumor necrosis factor (TNF) receptor or a ligand of a TNF receptor family member and at least a second binding site; and at least two polypeptide chains comprising at least one heavy chain portion and a synthetic connecting peptide; wherein greater than about 50% of the dimers comprise polypeptide chains that are linked via at least one interchain disulfide linkage.

The invention discloses an E.tenella protein disulfide linkage isomerase gene EtPDI (Clone ID is BW1-E06,and the Genbank accession number of is EF552214). The gene is connected with a procaryon expression vector pGEX-4T-2; a procaryon expression recombination plasmid pGEX-4T-EtPDI is constructed and is expressed in a colibacillus system; and most of the expressed recombining protein exists in a soluble form. The recombining protein 4T-EtPDI is purified to carry out SPS-PAGE and is transferred to a PVDF film; and antiserum of E.tenella oocyst oral immunized chicken is used as first resistance and goat anti-chicken IgG is used as second resistance to carry out Western-blot analysis, thereby indicating that the gene has certain antigen. The gene is used for preparing an anti-chicken coccidiosis drug and an anti-chicken coccidiosis vaccine.

There is disclosed a method and device for the delivery of molecules into individual cells. A device for injecting a biological molecule into a target cell comprises a microscopic tip attached to a mechanical scanning device for positioning the tip relative to the target cell and for moving the tip into the target cell; a nanostructure, such as a carbon nanotube, fixed on an end of the microscopic tip; and a biological molecule attached to the nanotube by a cleavable electrostatic or chemical linker linking the biomolecule to the nanotube, said chemical linker having a chemical linkage which is cleaved in an intracellular environment. The biological molecule may be one or more of proteins, nucleic acids, small molecule drugs, and optical labels, and combinations thereof. Exemplified are multiple walled carbon nanotubes to which a polycyclic aromatic compound is adsorbed, the aromatic compound having a side chain containing a cleavable disulfide linkage and a biotin functionality for coupling to a streptavidin-linked payload.

A composition including a C terminal region having residues corresponding to a peptide identified by PDB ID: 1RG3; an N terminal region having residues corresponding to a peptide identified by PDB ID: 1RG4; and a disulfide linkage between the residues near the C terminal region and the N terminal region. A composition including an exogenous peptide comprising amino acid residues comprising a C terminal region; amino acid residues comprising an N terminal region; a helix-loop-helix conformation between the residues comprising the C terminal region and the residues including the N terminal region; and at least one disulfide linkage between the residues comprising the C terminal region and the residues including N terminal region, wherein the residues including the C terminal region and the residues comprising the N terminal region have an amphiphatic property, and wherein the peptide has an a biological activity comparable to native surfactant protein SP-B. A method including delivering to a body a composition comprising an exogenous peptide having a biological activity comparable to native surfactant protein SP-B. A kit including an exogenous peptide having a biological activity comparable to native surfactant protein SP-B; and a treatment agent different from the peptide.

The invention discloses a thermostable amylase mutant and a preparation method thereof, which belongs to the field of genetic engineering. According to invention, bacillus alcalophilus JN21 (CCTCC NO:M2011231) amylase is used as female parent; molecular biological technique is adopted to conduct site-directed mutagenesis for bacillus alcalophilus amylase sequence; a pair or multiple pairs of disulfide linkages are introduced in amylase catalytic structural domain to obtain amylase mutant with higher thermal stability; under the modification condition, the half-life period of bacillus alcalophilus amylase at 60 DEG C is improved to 22.3 min from 3.2 min of a comparison example (before mutation); by utilizing the strategy, the thermostability of amylase can be obviously improved to provide basis for industrialized production, and the strategy has significant guiding significance for the modification of properties of other enzymes.

The present invention relates to non-aggregating VH domains or libraries thereof. The V_H domains comprise at least one disulfide linkage-forming cysteine in at least one complementarity-determining region (CDR) and an acidic isoelectric point (pI). A method of increasing the power or efficiency of selection of non-aggregating V_H domains comprises panning a phagemid-based V_H domain phage-display library in combination with a step of selecting non-aggregating phage-V_H domains. Compositions of matter comprising the non-aggregating V_H domains, as well as methods of use are also provided.

Provided in the present invention are a YAP protein inhibiting polypeptide and application thereof. In particular, the present invention obtains a key binding site of YAP protein and TEAD, screens a polypeptide with best YAP inhibitory activity and modifies the polypeptide, such as adding a disulfide linkage, replacing an amino acid, removing and/or adding (for example, adding a cell-penetrating element), and finally screens and verifies the obtaining of a series of polypeptides with YAP protein activity inhibiting effect and good stability. Experiments show that the polypeptide of the present invention can effectively inhibit the binding activity between YAP protein and TEAD, thus providing good therapeutic effect on digestive tract tumors (especially liver cancer).

Provided in the present invention are a YAP protein inhibiting polypeptide and application thereof. In particular, the present invention obtains a key binding site of YAP protein and TEAD, screens a polypeptide with best YAP inhibitory activity and modifies the polypeptide, such as adding a disulfide linkage, replacing an amino acid, removing and/or adding (for example, adding a cell-penetrating element), and finally screens and verifies the obtaining of a series of polypeptides with YAP protein activity inhibiting effect and good stability. Experiments show that the polypeptide of the present invention can effectively inhibit the binding activity between YAP protein and TEAD, thus providing good therapeutic effect on digestive tract tumors (especially liver cancer).

A label that permits rebridging of disulfide linkages in antibodies or proteins. The label has a general formula given by

wherein R₁ is methyl, ethyl or propyl and R₂ is a metal chelator, a fluorophore or a click-chemistry synthon.

The invention discloses a method for extracting vegetable tallow raft. The method comprises the following steps: (1) the plasma membrane is split to obtain lysis solution; (2) the density gradient centrifugation is carried out on the lysis solution in the step (1) to obtain the plant raft. The method uses dithiothreitol (DTT) to reduce the disulfide linkage, thus causing that the protein is fullydissolved. But phenylmethylsulfonyl fluoride (PMS) can effectively prevent the degradation of the protein in the extraction process. The method for extracting the plant raft has the advantages of being fast, simple and practicable and obtaining the tallow raft with higher quality and better repeatability. The method can play important role in the research of the vegetable tallow raft and has veryhigh practical using value.