55 results about "Bacillus alcalophilus" patented technology

The invention discloses a plant-fiber-loading-microbe self-repairing cement-based material. The plant-fiber-loading-microbe self-repairing cement-based material is prepared from a bacillus alcalophilus pasteurii bacterial solution, plant fibers and a nutrient solution, wherein the nutrient solution is prepared from maltodextrin, cottonseed cake meal, yeast extract powder and sodium citrate. The plant-fiber-loading-microbe self-repairing cement-based material disclosed by the invention has the following advantages that microbes can be preserved in a cement matrix for a long time in a high survival rate manner, so that the cement matrix can achieve life-time self-repairing in time of service; the adding of plants does not affect mechanical properties of concrete and can enhance the pull resistance and toughness of the concrete; the plants can release the microbes in time when cracks are generated; and the natural plants are wide in source, and the required plants do not need special complicated process treatment, so that the cost is low, and the plant-fiber-loading-microbe self-repairing cement-based material has environment-friendliness and economical efficiency.

The invention discloses a manufacturing process of grass cloth. The process comprises the steps of pre-stage preparation, degumming, warp yarn preparing, weaving, bleaching, dehydrating, dyeing, finishing, and packaging. According to the invention, ramie is subjected to the degumming process by using bacillus alcalophilus, such that ramie residual gum content can be effectively reduced, product safety is improved, and cloth dye uptake can be improved. With modification upon the bleaching process, cloth color stability is ensured, and bleaching duration can be reduced, such that working efficiency can be improved. The grass cloth manufacturing process provided by the invention is advantaged in low environmental pollution, high dye uptake, high color fastness, and short dyeing duration. With the process, economic benefit can be improved. The process is suitable for industrialized productions.

The invention discloses a bacterial strain capable of producing alkali protease and an industrialized liquid fermentation method of the bacterial strain, and belongs to the field of bioengineering technology. The bacterial strain is mutant strain Ap180 of Bacillus alcalophilus, and the preservation number is CGMCC No.7545. The bacterial strain is obtained by repeat UV-induced mutation, nitrosoguanidine-induced mutation and selection of a wild bacterial strain obtained from saline lands. Characteristics of the bacterial strain are that: efficiency of alkali protease production is high, and stability is excellent. The Bacillus alcalophilus strain, which is capable of producing alkali protease and is suitable for industrialized production , is provided in the invention, and related fermentation mechanism is optimized. According to the fermentation mechanism, operation processes are simple, cultivating conditions are mild, fermenting enzyme activity is more than 80000u/ml, production cost of enzyme activity per unit is reduced, and the requirements of market are met. Applications of the alkali protease in fields such as washing agent, forage, and leather industry are more extensive.

The invention relates to a microbial composition for the neutralization of alkaline waste waters by biological means and a method of neutralization of alkaline waste waters using a synergistic mixture of the bacterial strains of Bacillus alkalophilus and Bacillus sp.

The invention belongs to the field of interdiscipline of civil engineering materials and microbiology, and relates to a hydrogel encapsulated bacterial spore self-repairing material with pH responsiveness and a cement-based concrete self-repairing method. The self-repairing material comprises hydrogel with pH responsiveness, bacterial spores encapsulated by the hydrogel and a calcium source, wherein the hydrogel is prepared from a cross-linked hydrophilic polymer, and the bacterial spores comprise bacillus pseudofirmus spores. The hydrogel used in the invention has pH responsiveness, and can encapsulate bacterial spores on the premise of ensuring no damage to cement strength, thus successfully realizing cement self-healing.

The invention relates to a method for preparing feather meal additive via mixed solid-state fermentation, wherein adopted strains are bacillus licheniformis, bacillus alcalophilus and bacillus subtilis with preservation numbers of CGMCC (China General Microbiological Culture Collection Center) No.1699, CGMCC No.5313 and CGMCC No. 1398 respectively. According to the invention, as solid fermentation is adopted, broken feathers are processed to prepare solid fermentation medium; and the three strains are mixed according to a certain proportion and then inoculated into the fermentation medium according to suitable inoculum size. According to the method provided by the invention, the hydrolysis efficiency of feather can be effectively improved and the content of soluble protein can be up to 9.42mg/g, so that a high-nutrition feed additive is provided for the feed industry and the resource waste and environment pollution are effectively avoided to a certain extent.

The invention provides a method for preparing an alkaline protease by a fermentation method. The method comprises the following steps of: obtaining a high-yield bacillus alcalophilus strain Svf4-21-7 stable in heritability through ultraviolet-NET compound mutation treatment; carrying out the following treatment steps on the fermentation broth: salting out by using ammonium sulfate, dialysis and desalination, ion-exchange column chromatography, polyethylene glycol embedment and enrichment and gel filtering chromatography, thus obtaining the alkaline protease having activity greater than or equal to 100000 u/g after being freeze-dried. The alkaline protease prepared by the method provided by the invention is high in yield, and the preparation process is relatively simple; therefore, the alkaline protease is suitable for industrial production.

The invention discloses a thermostable amylase mutant and a preparation method thereof, which belongs to the field of genetic engineering. According to invention, bacillus alcalophilus JN21 (CCTCC NO:M2011231) amylase is used as female parent; molecular biological technique is adopted to conduct site-directed mutagenesis for bacillus alcalophilus amylase sequence; a pair or multiple pairs of disulfide linkages are introduced in amylase catalytic structural domain to obtain amylase mutant with higher thermal stability; under the modification condition, the half-life period of bacillus alcalophilus amylase at 60 DEG C is improved to 22.3 min from 3.2 min of a comparison example (before mutation); by utilizing the strategy, the thermostability of amylase can be obviously improved to provide basis for industrialized production, and the strategy has significant guiding significance for the modification of properties of other enzymes.

The invention provides a microbial agent and applications thereof, and relates to the technical field of biological agents. The microbial agent comprises citric acid fermentation tail liquid, peptone,yeast powder, bacillus alcaligenes and water; wherein the volume ratio of the citric acid fermentation tail liquid to the bacillus alcaligenes to the water to the peptone to the yeast powder is (15-25)mL: (2-3) mL:(975-985) mL:(8-12) g: (4-6) g; the content of organic matters in the citric acid fermentation tail liquid is 150-250g/L. The microbial agent provided by the invention not only has a remarkable effect of improving saline-alkali soil, but also can promote crop growth, increase crop yield and improve fruit quality.

The invention discloses an oxidation resistive amylase mutant as well as a preparation method and application thereof, and belongs to the field of genetic engineering. According to the invention, the bacillus alcalophilus amylase sequence is mutated in directed site by using molecular biological technique based on bacillus alcalophilus JN21 (CCTCC NO:M2011231) amylase as a female parent. In the modifying condition, the bacillus alcalophilus amylas is treated for 5 hours at 40 DEG C in 500mM H2O2, and enzymatic activity residue is changed from 10% (before mutation) to 72%. The oxidation resistance of amylase can be greatly improved through the policy so as to lay a foundation in the industrial production fields such as spinning desizing and detergent and additive. The policy has an import guiding meaning for modifying the oxidation resistance of amylase and other enzymes.

The invention relates to a multifunctional shuttle vector new pBE2, a construction method thereof and a method for constructing an alkali protease mutation library by using the same. The main structure of the multifunctional shuttle vector is LB-P43-SP-MCS-RB. The construction method mainly comprises the following steps: connecting a big fragment of the pBE2 vector with a P43 strong promoter and the product of the amplification of surfactant protein (SP) and introducing a BamHI enzyme cutting site. The method for constructing the alkali protease mutation library mainly comprises: connecting a mature peptide fragment of an alkali protease gene of bacillus alcalophilus to the new pBE2, transferring into Escherichia coli to obtain a positive clone, transferring the positive clone into bacillus subtilis WB600 and performing several circles of high-flux screening. When the method for constructing the alkali protease mutation library, which is provided by the invention, is used, the pertinence to mutation of an alkali protease gene is increased, mutation is performed according to the mature peptide part of the alkali protease, and the workload required for oriented evolution study on the alkali protease is reduced greatly.

The invention discloses a microorganism powder product for treating heavy metal polluted saline-alkali soil and a manufacturing method thereof. The powder product is composed of 50-70 parts of bacteria-containing corn flour, 15-25 parts of bacteria-containing rice bran powder, 10-20 parts of bacteria-containing peanut cake powder, and 5-10 parts of bacteria-containing soybean cake powder. Bacillus alcalophilus is cultured in the bacteria-containing corn flour with bacterial concentration being 1*10<10>-2*10<10> cfu/g; bacillus subtilis is cultured in the bacteria-containing rice bran powder with bacterial concentration being 5*10<9>-2*10<10> cfu/g; white rot fungi is cultured in the bacteria-containing peanut cake powder with bacterial concentration being 1*10<9>-1*10<10> cfu/g; bacillius firmus is cultured in the bacteria-containing soybean cake powder with bacterial concentration being 5*10<9>-2*10<10> cfu/g. The invention also discloses the manufacturing method of the microorganism powder product. The microorganism powder product is used for heavy metal polluted saline-alkali soil.

The invention relates to a method for shortening leather making soaking time. When Na+ and Ca2+ are used for leather soaking, protease and lipase are added to be used as an auxiliary, the concentration of Na+ and the concentration of Ca2+ in the soaking procedure are adjusted, and the protease is produced by bacillus amyloliquefaciens CGMCC No.11218 and bacillus alcalophilus high-producing strains CGMCC No.6537. The treatment time of a traditional soaking procedure is shortened, and the usage amount of sodium sulphide in the subsequent depilation process is lowered.

The present invention provides a method for preparing alkaline protease through a fermentation method, wherein an ultraviolet ray-NTG composite mutagenesis treatment is adopted to obtain a high yield alkaliphilic Bacillus alcalophilus strain Svf4-21-7 with stable genetic property, a fermentation broth is subjected to ammonium sulfate salting-out, dialysis desalting, ion exchange chromatography, polyethylene glycol embedding concentration and gel filtration chromatography, and activity of the lyophilized alkaline protease is more than or equal to 100000 u/g. According to the present invention, a yield of the prepared alkaline protease is high, the process is relatively simple, and the method is suitable for industrial production.

The invention relates to a microbial foam extinguishing agent and mainly aims to solve the problem of environmental pollution existing in the prior art. The microbial foam extinguishing agent disclosed by the invention comprises microbes, a surfactant, a cosolvent and an additive, wherein the microbes are bacillus strains separated from soil; the bacillus is at least one of bacillus subtilis, bacillus macerans, bacillus alcalophilus, bacillus polymyxa, bacillus licheniformis, bacillus mycoides, bacillus natto and bacillus amyloliquefaciens; the surfactant is a compound of a hydrocarbon surfactant and a fluorocarbon surfactant and contains at least one fluorocarbon surfactant; the cosolvent is diethylene glycol momobutyl ether or triethylene glycol momobutyl ether; and the additive contains a stabilizing agent, a thickening agent, an anti-corrosive agent and inorganic nutritional substances. The technical scheme of the microbial foam extinguishing agent preferably solves the problems and the microbial foam extinguishing agent can be applied to fire protection and fire extinguishment.

The invention discloses Bacillus alcalophilus NTT33C6, collected under CCTCC M2016316 and capable of producing constitutive alkaline Beta-mannase; the bacterium is rod shaped, is 0.5-0.65*2.6-3.4 Mum in size, with lateral flagella, is mobile and produces elliptical endogenous spores; the bacterium is aerobic, can form a circular colony, has smooth surface, and is concentric; Bacillus alcalophilus NTT33C6 is capable of biologically degumming hemp, with a degumming rate in 6-8 hours reaching 70% and above, and capable of biologically degumming hemp with skin not removed, with a degumming rate in 6-8 hours reaching 80% and above; degumming wastewater is easy to treat; biological degumming and chemical degumming are jointly used, and refined dried hemp with certain need on residual gum content can be produced on production demand.

The invention discloses an amylase mutant with improved heat stability and application thereof and belongs to the field of genetic engineering. The amylase mutant uses Bacillus alcalophilus JN21 (CCTCC NO:M 2011231) amylase as a female parent, and a molecular biological technique is adopted to perform site-specific mutagenesis to a Bacillus alcalophilus amylase sequence. Under the transformation condition, the Bacillus alcalophilus amylase is improved from 15.3min (before mutation) to 43.8min at a 50-DEG C half-life period. By means of the application, the heat stability of the amylase can be improved remarkably, and a foundation is provided for industrial production of the amylase. The application has important guiding significance on modification of other enzymes.

The invention discloses a method for preparing recombined bacillus alcalophilus. According to the method, bacillus alcalophilus for producing L-lactic acid is used as original strain, and by means of genetic engineering operation, the original strain is made not to express L-lactic dehydrogenase but to express D-lactic dehydrogenase. The invention further discloses recombined bacillus alcalophilus prepared with the method, application of the recombined bacillus alcalophilus, and a method for preparing D-lactic acid. When the recombined bacillus alcalophilus prepared with the method for preparing the recombined bacillus alcalophilus is applied to preparation of D-lactic acid, the optical purity of the obtained D-lactic acid is higher than 99.8%, the conversion rate is 94% or more, and the yield can reach 142 g/L.

The invention provides a bacillus alcalophilus strain wp-1 and application thereof, and relates to the technical field of agriculture microbes. The bacillus alcalophilus strain wp-1 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO:M 2018427. The bacillus basophilus strain wp-1 has the ability to tolerate salt and alkaline environments. The bacillus basophilus strain wp-1 has the ability to convert insoluble phosphate into soluble phosphorus which can be absorbed by plants, can further synthesize indoleacetic acid (IAA) by using tryptophan, and has the ability to synthesize iron carriers. The bacillus basophilus strain wp-1 can decrease the malondialdehyde content in wheat roots, and can be used for improving plant stress resistance. Thebacillus basophilus strain wp-1 further has the effect of promoting the plants to grow in non-stress environments and salt-stress environments.

The invention discloses an oil stain biodegradation element which comprises the following components in parts by weight: 15-17 parts of mixed bacteria fluid, 3.5-4.5 parts of normal saline, 35-45 parts of cotton seed hulls and 35-45 parts of solid organic fertilizer, wherein the mixed bacteria fluid comprises the following components in parts by weight: 0.9-1.1 parts of bacillus alcalophilus fluid in which the active viable count is not less than 1 hundred million pieces/ml, 0.9-1.1 parts of alcanivorax fluid in which the active viable count is not less than 1 hundred million pieces/ml and 0.9-1.1 parts of cycloclasticus fluid in which the active viable count is not less than 1 hundred million pieces/ml. The invention also discloses a preparation method and an application method for the oil stain biodegradation element. According to the invention, the oil stain biodegradation element is adopted for treating oily sludge, the equipment is simple, the operation is convenient, the cost is low, no pollution exists and the application range is wide.

The invention aims at providing a composite flora for processing food waste grease. The composite flora contains pseudomonas fluorescens and bacillus alcalophilus, wherein the pseudomonas fluorescens is preferably the pseudomonas fluorescens with the collection number being 1.3990 collected in the CGMCC (China General Microbiological Culture Collection Center); the collection number of the bacillus alcalophilus is CGMCC No.9430. Through the composite flora, the addition of the microbial inoculums XS2-450 has the positive influence on the food waste grease degradation; in addition, the degradation capability of pseudomonas fluorescens 1.3990 on the grease can be greatly improved, and is improved from 60 percent to 92 percent.