55 results about "Bacillus alcalophilus" patented technology

The invention discloses a plant-fiber-loading-microbe self-repairing cement-based material. The plant-fiber-loading-microbe self-repairing cement-based material is prepared from a bacillus alcalophilus pasteurii bacterial solution, plant fibers and a nutrient solution, wherein the nutrient solution is prepared from maltodextrin, cottonseed cake meal, yeast extract powder and sodium citrate. The plant-fiber-loading-microbe self-repairing cement-based material disclosed by the invention has the following advantages that microbes can be preserved in a cement matrix for a long time in a high survival rate manner, so that the cement matrix can achieve life-time self-repairing in time of service; the adding of plants does not affect mechanical properties of concrete and can enhance the pull resistance and toughness of the concrete; the plants can release the microbes in time when cracks are generated; and the natural plants are wide in source, and the required plants do not need special complicated process treatment, so that the cost is low, and the plant-fiber-loading-microbe self-repairing cement-based material has environment-friendliness and economical efficiency.

The invention discloses a manufacturing process of grass cloth. The process comprises the steps of pre-stage preparation, degumming, warp yarn preparing, weaving, bleaching, dehydrating, dyeing, finishing, and packaging. According to the invention, ramie is subjected to the degumming process by using bacillus alcalophilus, such that ramie residual gum content can be effectively reduced, product safety is improved, and cloth dye uptake can be improved. With modification upon the bleaching process, cloth color stability is ensured, and bleaching duration can be reduced, such that working efficiency can be improved. The grass cloth manufacturing process provided by the invention is advantaged in low environmental pollution, high dye uptake, high color fastness, and short dyeing duration. With the process, economic benefit can be improved. The process is suitable for industrialized productions.

The invention discloses a bacterial strain capable of producing alkali protease and an industrialized liquid fermentation method of the bacterial strain, and belongs to the field of bioengineering technology. The bacterial strain is mutant strain Ap180 of Bacillus alcalophilus, and the preservation number is CGMCC No.7545. The bacterial strain is obtained by repeat UV-induced mutation, nitrosoguanidine-induced mutation and selection of a wild bacterial strain obtained from saline lands. Characteristics of the bacterial strain are that: efficiency of alkali protease production is high, and stability is excellent. The Bacillus alcalophilus strain, which is capable of producing alkali protease and is suitable for industrialized production , is provided in the invention, and related fermentation mechanism is optimized. According to the fermentation mechanism, operation processes are simple, cultivating conditions are mild, fermenting enzyme activity is more than 80000u / ml, production cost of enzyme activity per unit is reduced, and the requirements of market are met. Applications of the alkali protease in fields such as washing agent, forage, and leather industry are more extensive.

The invention relates to bacillus alcalophilus and an application thereof to fermentative hydrogen production. The steps of sludge and / or organic waste pretreatment and fermentative hydrogen production are adopted. The bacillus alcalophilus is characterized in that in the sludge and / or organic waste pretreatment step, bacillus alcalophilus liquid is inoculated in sludge and / or organic wastes, and bacillus alcalophilus hydrolysis pretreatment sludge and / or organic wastes are obtained after the treatment. In addition, the prepared bacillus alcalophilus hydrolysis pretreatment sludge can also be used as hydrogen production floras to be inoculated into the organic wastes for producing hydrogen through fermentation. A bacillus alcalophilus sludge pretreatment method can be effectively used for screening efficient hydrogen production floras in the sludge, inhabiting the growth of hydrogen consumption bacteria and killing pathogenic bacteria in the sludge and / or organic wastes. The sludge and / or organic wastes through all bacterial strain treatment has / have the large hydrogen generation quantity in the subsequent fermentative hydrogen production step, the purity of hydrogen gas is high, the delay time of the hydrogen production is short, and obvious economic values and development potential are realized.

The invention provides a method for producing galacto-mannan-oligosaccharide by the enzymic degradation of locust bean gum. The method is characterized in that: preparing enzyme-producing culture medium, inoculating bacillus alcalophilus, fermenting, extracting coarse enzyme liquid of mannase, dissolving the locust bean gum in buffer solution, adding the coarse enzyme liquid of the mannase into the solution for enzymatic degradation, inactivating the mannase by boiling water bath heating, removing deposited impurities after centrifuging, making the precipitation fractionation by organic solvent after concentration, and obtaining the galacto-mannan-oligosaccharide fragment after freeze drying. The preparation method has the advantages of low requirement on environmental equipment, simple and easy operation, low cost, simple composition of the product, mild preparation conditions, good structural stability of the product and suitability for popularization.

The invention discloses a preparation method of compression-resistant self-repairing concrete, belonging to the technical field of construction material preparation. The repairing packing prepared bythe invention is bacillus alcalophilus-carried epoxy-fluorocarbon composite resin micro-capsules; the adopted bacillus alcalophilus has a good adaptability for high alkaline environment in concrete; and moreover, the self-repairing packing prepared by the invention can be used for self-diagnosing and self-repairing concrete cracks, thereby reaching the purpose of crack self-repairing. Bacillus alcalophilus, the remediating inoculant, in the compression-resistant self-repairing concrete prepared by the invention is screened and cultured through alkaline environment culture media of secondary settling sludge diluent, calcium bicarbonate and sodium carbonate; the doped carbon nanotubes can enhance siphon action produced by fracture surfaces of cracks, so that concrete cracks can be basicallyrepaired fast, and then be strengthened and repaired by calcium bicarbonate in concrete that forms into calcium carbonate slowly, and moreover, capsule core, as fluid packing, is good in thixotropic property and can improve compression-resistant performance of concrete.

The invention discloses a Bacillus alcalophilus SD 7903 with the preservation number of CGMCC No. 3158, belonging to the field of microbial technology; the invention also discloses a culture method thereof and an application in producing Ectoine. The Bacillus alcalophilus has higher Ectoine synthesis capability and is a strain of Gram positive bacteria, thus expanding biological resource utilization space and having good industrial application prospect in respect to production of Ectoine mainly by applying Gram negative bacteria in the current industry.

The invention relates to a low-temperature alkaline protease and a preparation method thereof, belongs to a method of performing site-directed mutagenesis to a wild type alkaline protease gene by utilizing a recombinant DNA (deoxyribonucleic acid) technology to improve the characteristics of the gene, connecting the mutated gene with escherichia coli-bacillus subtilis shuttle plasmid pBE2S and expressing the mutated gene in the bacillus subtilis at high efficiency, and relates to a low-temperature alkaline protease with cold adaptability and alkali stability and a preparation method thereof. The invention solves the problem that the alkaline protease has so low activity in a low-temperature environment that the application is limited. The invention adopts a technical scheme that: the wildtype alkaline protease gene is separated from a microbe, in particular bacillus alcalophilus; amino acid residues of Glu110 and Glu134 of the gene are mutated; the enzyme activity of fermentation fluid of the gene is 1985 U / mL after the gene is expressed in the bacillus subtilis at high efficiency; at 40 DEG C, the activity of the low-temperature alkaline protease (Glu110 and 134 Ala) is improvedby 28 percent, compared with that of the wild type alkaline protease; and at 10 DEG C, the activity is improved by 62 percent, compared with that of the wild type alkaline protease.

The invention relates to a microbial composition for the neutralization of alkaline waste waters by biological means and a method of neutralization of alkaline waste waters using a synergistic mixture of the bacterial strains of Bacillus alkalophilus and Bacillus sp.

The invention belongs to the field of interdiscipline of civil engineering materials and microbiology, and relates to a hydrogel encapsulated bacterial spore self-repairing material with pH responsiveness and a cement-based concrete self-repairing method. The self-repairing material comprises hydrogel with pH responsiveness, bacterial spores encapsulated by the hydrogel and a calcium source, wherein the hydrogel is prepared from a cross-linked hydrophilic polymer, and the bacterial spores comprise bacillus pseudofirmus spores. The hydrogel used in the invention has pH responsiveness, and can encapsulate bacterial spores on the premise of ensuring no damage to cement strength, thus successfully realizing cement self-healing.

The invention relates to a method for preparing feather meal additive via mixed solid-state fermentation, wherein adopted strains are bacillus licheniformis, bacillus alcalophilus and bacillus subtilis with preservation numbers of CGMCC (China General Microbiological Culture Collection Center) No.1699, CGMCC No.5313 and CGMCC No. 1398 respectively. According to the invention, as solid fermentation is adopted, broken feathers are processed to prepare solid fermentation medium; and the three strains are mixed according to a certain proportion and then inoculated into the fermentation medium according to suitable inoculum size. According to the method provided by the invention, the hydrolysis efficiency of feather can be effectively improved and the content of soluble protein can be up to 9.42mg / g, so that a high-nutrition feed additive is provided for the feed industry and the resource waste and environment pollution are effectively avoided to a certain extent.

The invention relates to a method for producing an alkali protease. The classification name of bacterial strains is Bacillus alcalophilus, and the preservation number is CGMCC No.5313. According to the fermentation process in the invention, clear liquid fermentation is combined with fed-batch fermentation, a cottonseed cake powder enzymatic hydrolysate is prepared from crude cottonseed cake powder, and a fed-batch carbon nitrogen source fermentation mode is adopted, so the fermentation enzyme activity is effectively improved, the enzyme activity of a 7L fermentation tank, which reaches 41000U / mL, is 13.9% higher than the original enzyme activity of 36000U / mL of the 7L fermentation tank, the late enzyme separation purifying difficulty is reduced, the production cost is reduced, and good economic benefits are obtained.

The invention provides a method for preparing an alkaline protease by a fermentation method. The method comprises the following steps of: obtaining a high-yield bacillus alcalophilus strain Svf4-21-7 stable in heritability through ultraviolet-NET compound mutation treatment; carrying out the following treatment steps on the fermentation broth: salting out by using ammonium sulfate, dialysis and desalination, ion-exchange column chromatography, polyethylene glycol embedment and enrichment and gel filtering chromatography, thus obtaining the alkaline protease having activity greater than or equal to 100000 u / g after being freeze-dried. The alkaline protease prepared by the method provided by the invention is high in yield, and the preparation process is relatively simple; therefore, the alkaline protease is suitable for industrial production.

The invention discloses a thermostable amylase mutant and a preparation method thereof, which belongs to the field of genetic engineering. According to invention, bacillus alcalophilus JN21 (CCTCC NO:M2011231) amylase is used as female parent; molecular biological technique is adopted to conduct site-directed mutagenesis for bacillus alcalophilus amylase sequence; a pair or multiple pairs of disulfide linkages are introduced in amylase catalytic structural domain to obtain amylase mutant with higher thermal stability; under the modification condition, the half-life period of bacillus alcalophilus amylase at 60 DEG C is improved to 22.3 min from 3.2 min of a comparison example (before mutation); by utilizing the strategy, the thermostability of amylase can be obviously improved to provide basis for industrialized production, and the strategy has significant guiding significance for the modification of properties of other enzymes.

The invention provides a microbial agent and applications thereof, and relates to the technical field of biological agents. The microbial agent comprises citric acid fermentation tail liquid, peptone,yeast powder, bacillus alcaligenes and water; wherein the volume ratio of the citric acid fermentation tail liquid to the bacillus alcaligenes to the water to the peptone to the yeast powder is (15-25)mL: (2-3) mL:(975-985) mL:(8-12) g: (4-6) g; the content of organic matters in the citric acid fermentation tail liquid is 150-250g / L. The microbial agent provided by the invention not only has a remarkable effect of improving saline-alkali soil, but also can promote crop growth, increase crop yield and improve fruit quality.

The invention discloses a sludge compost thermophilic microbial agent and application thereof. The sludge compost thermophilic microbial agent comprises 2 to 8*10<10> cfu / g of Bacillus alcalophilus, 3 to 6*10<10> cfu / g of Thermophilic Lactobacillus, 4 to 6*10<11> cfu / g of Bacillus subtilis and 2 to 6*10<11> cfu / g of Thermophilic bacteria Lian. The sludge compost thermophilic microbial agent disclosed by the invention has the functions of promoting the biochemical sludge aerobic composting process, prolonging the compost high temperature period, and enhancing the compost maturity, so as to be capable of accelerating the biodegradation of organic matters in raw materials. The sludge compost thermophilic microbial agent can be used in compost enterprises for composting biochemical sludge of municipal sewage plants to produce bioorganic fertilizers and further reduce environmental hazards caused by the sludge.

The invention discloses an oxidation resistive amylase mutant as well as a preparation method and application thereof, and belongs to the field of genetic engineering. According to the invention, the bacillus alcalophilus amylase sequence is mutated in directed site by using molecular biological technique based on bacillus alcalophilus JN21 (CCTCC NO:M2011231) amylase as a female parent. In the modifying condition, the bacillus alcalophilus amylas is treated for 5 hours at 40 DEG C in 500mM H2O2, and enzymatic activity residue is changed from 10% (before mutation) to 72%. The oxidation resistance of amylase can be greatly improved through the policy so as to lay a foundation in the industrial production fields such as spinning desizing and detergent and additive. The policy has an import guiding meaning for modifying the oxidation resistance of amylase and other enzymes.

The invention relates to a multifunctional shuttle vector new pBE2, a construction method thereof and a method for constructing an alkali protease mutation library by using the same. The main structure of the multifunctional shuttle vector is LB-P43-SP-MCS-RB. The construction method mainly comprises the following steps: connecting a big fragment of the pBE2 vector with a P43 strong promoter and the product of the amplification of surfactant protein (SP) and introducing a BamHI enzyme cutting site. The method for constructing the alkali protease mutation library mainly comprises: connecting a mature peptide fragment of an alkali protease gene of bacillus alcalophilus to the new pBE2, transferring into Escherichia coli to obtain a positive clone, transferring the positive clone into bacillus subtilis WB600 and performing several circles of high-flux screening. When the method for constructing the alkali protease mutation library, which is provided by the invention, is used, the pertinence to mutation of an alkali protease gene is increased, mutation is performed according to the mature peptide part of the alkali protease, and the workload required for oriented evolution study on the alkali protease is reduced greatly.

The invention discloses a microorganism powder product for treating heavy metal polluted saline-alkali soil and a manufacturing method thereof. The powder product is composed of 50-70 parts of bacteria-containing corn flour, 15-25 parts of bacteria-containing rice bran powder, 10-20 parts of bacteria-containing peanut cake powder, and 5-10 parts of bacteria-containing soybean cake powder. Bacillus alcalophilus is cultured in the bacteria-containing corn flour with bacterial concentration being 1*10<10>-2*10<10> cfu / g; bacillus subtilis is cultured in the bacteria-containing rice bran powder with bacterial concentration being 5*10<9>-2*10<10> cfu / g; white rot fungi is cultured in the bacteria-containing peanut cake powder with bacterial concentration being 1*10<9>-1*10<10> cfu / g; bacillius firmus is cultured in the bacteria-containing soybean cake powder with bacterial concentration being 5*10<9>-2*10<10> cfu / g. The invention also discloses the manufacturing method of the microorganism powder product. The microorganism powder product is used for heavy metal polluted saline-alkali soil.

The invention relates to a method for shortening leather making soaking time. When Na+ and Ca2+ are used for leather soaking, protease and lipase are added to be used as an auxiliary, the concentration of Na+ and the concentration of Ca2+ in the soaking procedure are adjusted, and the protease is produced by bacillus amyloliquefaciens CGMCC No.11218 and bacillus alcalophilus high-producing strains CGMCC No.6537. The treatment time of a traditional soaking procedure is shortened, and the usage amount of sodium sulphide in the subsequent depilation process is lowered.

The present invention provides a method for preparing alkaline protease through a fermentation method, wherein an ultraviolet ray-NTG composite mutagenesis treatment is adopted to obtain a high yield alkaliphilic Bacillus alcalophilus strain Svf4-21-7 with stable genetic property, a fermentation broth is subjected to ammonium sulfate salting-out, dialysis desalting, ion exchange chromatography, polyethylene glycol embedding concentration and gel filtration chromatography, and activity of the lyophilized alkaline protease is more than or equal to 100000 u / g. According to the present invention, a yield of the prepared alkaline protease is high, the process is relatively simple, and the method is suitable for industrial production.

The invention relates to a microbial foam extinguishing agent and mainly aims to solve the problem of environmental pollution existing in the prior art. The microbial foam extinguishing agent disclosed by the invention comprises microbes, a surfactant, a cosolvent and an additive, wherein the microbes are bacillus strains separated from soil; the bacillus is at least one of bacillus subtilis, bacillus macerans, bacillus alcalophilus, bacillus polymyxa, bacillus licheniformis, bacillus mycoides, bacillus natto and bacillus amyloliquefaciens; the surfactant is a compound of a hydrocarbon surfactant and a fluorocarbon surfactant and contains at least one fluorocarbon surfactant; the cosolvent is diethylene glycol momobutyl ether or triethylene glycol momobutyl ether; and the additive contains a stabilizing agent, a thickening agent, an anti-corrosive agent and inorganic nutritional substances. The technical scheme of the microbial foam extinguishing agent preferably solves the problems and the microbial foam extinguishing agent can be applied to fire protection and fire extinguishment.

The invention discloses Bacillus alcalophilus NTT33C6, collected under CCTCC M2016316 and capable of producing constitutive alkaline Beta-mannase; the bacterium is rod shaped, is 0.5-0.65*2.6-3.4 Mum in size, with lateral flagella, is mobile and produces elliptical endogenous spores; the bacterium is aerobic, can form a circular colony, has smooth surface, and is concentric; Bacillus alcalophilus NTT33C6 is capable of biologically degumming hemp, with a degumming rate in 6-8 hours reaching 70% and above, and capable of biologically degumming hemp with skin not removed, with a degumming rate in 6-8 hours reaching 80% and above; degumming wastewater is easy to treat; biological degumming and chemical degumming are jointly used, and refined dried hemp with certain need on residual gum content can be produced on production demand.

The invention discloses an oxidation-resistant amylase mutant and a preparation method thereof, and belongs to the field of genetic engineering. The preparation method of the invention adopts Bacillus alcalophilus JN21 (CCTCC NO: M 2011231) amylase as a female parent, and performs site-directed mutagenesis of the Bacillus alcalophilus amylase sequence by molecular biology technology. Under the reconstruction conditions, the Bacillus alcalophilus amylase is treated at 40 DEG C in 500 mM H2O2 environment for 30 min, and the enzymatic activity residue is increased from 18% of a control (before mutation) case to 92%; with the strategy, the oxidation resistance of the amylase is greatly increased, which lays a foundation for the application of the amylase in industrial production fields of weaving desizing, washing agent additives, and the like. The strategy has important guidance meaning for the reconstruction of oxidation resistance of amylase and other enzymes.

The invention discloses an amylase mutant with improved heat stability and application thereof and belongs to the field of genetic engineering. The amylase mutant uses Bacillus alcalophilus JN21 (CCTCC NO:M 2011231) amylase as a female parent, and a molecular biological technique is adopted to perform site-specific mutagenesis to a Bacillus alcalophilus amylase sequence. Under the transformation condition, the Bacillus alcalophilus amylase is improved from 15.3min (before mutation) to 43.8min at a 50-DEG C half-life period. By means of the application, the heat stability of the amylase can be improved remarkably, and a foundation is provided for industrial production of the amylase. The application has important guiding significance on modification of other enzymes.

The invention discloses a method for preparing recombined bacillus alcalophilus. According to the method, bacillus alcalophilus for producing L-lactic acid is used as original strain, and by means of genetic engineering operation, the original strain is made not to express L-lactic dehydrogenase but to express D-lactic dehydrogenase. The invention further discloses recombined bacillus alcalophilus prepared with the method, application of the recombined bacillus alcalophilus, and a method for preparing D-lactic acid. When the recombined bacillus alcalophilus prepared with the method for preparing the recombined bacillus alcalophilus is applied to preparation of D-lactic acid, the optical purity of the obtained D-lactic acid is higher than 99.8%, the conversion rate is 94% or more, and the yield can reach 142 g / L.

The invention provides a bacillus alcalophilus strain wp-1 and application thereof, and relates to the technical field of agriculture microbes. The bacillus alcalophilus strain wp-1 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO:M 2018427. The bacillus basophilus strain wp-1 has the ability to tolerate salt and alkaline environments. The bacillus basophilus strain wp-1 has the ability to convert insoluble phosphate into soluble phosphorus which can be absorbed by plants, can further synthesize indoleacetic acid (IAA) by using tryptophan, and has the ability to synthesize iron carriers. The bacillus basophilus strain wp-1 can decrease the malondialdehyde content in wheat roots, and can be used for improving plant stress resistance. Thebacillus basophilus strain wp-1 further has the effect of promoting the plants to grow in non-stress environments and salt-stress environments.

The invention discloses an oil stain biodegradation element which comprises the following components in parts by weight: 15-17 parts of mixed bacteria fluid, 3.5-4.5 parts of normal saline, 35-45 parts of cotton seed hulls and 35-45 parts of solid organic fertilizer, wherein the mixed bacteria fluid comprises the following components in parts by weight: 0.9-1.1 parts of bacillus alcalophilus fluid in which the active viable count is not less than 1 hundred million pieces / ml, 0.9-1.1 parts of alcanivorax fluid in which the active viable count is not less than 1 hundred million pieces / ml and 0.9-1.1 parts of cycloclasticus fluid in which the active viable count is not less than 1 hundred million pieces / ml. The invention also discloses a preparation method and an application method for the oil stain biodegradation element. According to the invention, the oil stain biodegradation element is adopted for treating oily sludge, the equipment is simple, the operation is convenient, the cost is low, no pollution exists and the application range is wide.

The invention provides an alkalophilic bacillus strain wp-1 and its application, and relates to the technical field of agricultural microorganisms. The alkalophilic bacillus strain wp-1 is preserved in the China Center for Type Culture Collection with a preservation number of CCTCC NO: M 2018427. The alkalophilic bacillus strain wp‑1 of the present invention has the ability to tolerate salt and alkali environments. The alkalophilic Bacillus strain wp‑1 has the ability to convert insoluble phosphate into soluble phosphorus that can be absorbed by plants, and can also synthesize indoleacetic acid (IAA) using tryptophan, and has the ability to synthesize siderophore. The alkalophilic bacillus strain wp-1 of the invention can reduce the content of malondialdehyde in wheat roots, and can be used to improve the stress resistance of plants. The alkalophilic bacillus strain wp-1 of the present invention also has the effect of promoting plant growth in a non-stress environment and a salt stress environment.

The invention aims at providing a composite flora for processing food waste grease. The composite flora contains pseudomonas fluorescens and bacillus alcalophilus, wherein the pseudomonas fluorescens is preferably the pseudomonas fluorescens with the collection number being 1.3990 collected in the CGMCC (China General Microbiological Culture Collection Center); the collection number of the bacillus alcalophilus is CGMCC No.9430. Through the composite flora, the addition of the microbial inoculums XS2-450 has the positive influence on the food waste grease degradation; in addition, the degradation capability of pseudomonas fluorescens 1.3990 on the grease can be greatly improved, and is improved from 60 percent to 92 percent.