The invention provides a sialic acid assay kit, comprising a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from the following components with corresponding concentrations: 8-20g/L tris(hydroxymethyl) aminomethane, 5-15ml/L HCL, 0.5-5KU/L neuraminidase, 0.5-5KU/L lactate dehydrogenase and 0.1-2mmol/L NADH; the reagent R2 is prepared from the following components with correspondingconcentrations: 8-20g/L tris(hydroxymethyl) aminomethane, 5-15ml/L HCL, 0.5-5KU/L N-acetylneuraminic acid aldolase, 25-100g/L sucrose and 0.6-1.2 ml/L triethanolamine. The invention belongs to the technical field of biological detection. The sialic acid assay kit provided by the invention significantly enhances the anti-interference ability while improving the stability, can significantly reducethe interference of bilirubin, hemoglobin, triglyceride and the like, and is good in accuracy when being used for the determination of sialic acid.