Homocysteine ​​determination kit, preparation method and detection method thereof

A technology for homocysteine ​​and kits, which is applied in the field of homocysteine ​​determination kits and its preparation, can solve the problems of HCY kit sensitivity and open cap stability time to be improved, and achieve good linear correlation , high accuracy and high sensitivity

Active Publication Date: 2021-08-10
WUHAN CHANGXINSHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sensitivity and cap-opening stability time of the HCY kit used in the current conventional cycle enzymatic method still need to be improved

Method used

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  • Homocysteine ​​determination kit, preparation method and detection method thereof
  • Homocysteine ​​determination kit, preparation method and detection method thereof
  • Homocysteine ​​determination kit, preparation method and detection method thereof

Examples

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Embodiment 1

[0040] This embodiment provides a homocityrocysteine ​​(HCY) assay kit (circulating enzyme method), comprising a separate packaged reagent R1, a reagent R2, and a calibrator, wherein the reagent R1 is prepared by each component of the following concentration. Aqueous solution:

[0041]

[0042] The reagent R2 is aqueous solution prepared from each component of the following concentration:

[0043]

[0044] The calibration was selected from the Hcy calibration produced by Beijing Century Ward Biotechnology Co., Ltd., concentration of 28 μmol / L.

[0045] The method of preparing the same type cysteine ​​(Hcy) assay kit (circulating enzyme method) provided in this example, including the following steps:

[0046] Formulation reagent R1: In accordance with the composition weighing of the reagent R1 of the present embodiment, it is added to the purified water of 4 / 5-fold pre-prepared solution volume, and dihydrodium dihydrophide sodium dihydrodoxide, stirring, dissolution. The adju...

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Abstract

The present invention relates to a homocysteine ​​assay kit and its preparation method and detection method. The homocysteine ​​assay kit includes reagent R1 and reagent R2. Reagent R1 is prepared from the following components: Aqueous solution: phosphate buffer, 1.8~2.2mL / L Triton X‑100, 0.11~0.16g / L L‑serine, 805~815KU / L lactate dehydrogenase, 0.5~0.75g / L NADH and 0.8-1.2mL / L ProcLin300; reagent R2 is an aqueous solution prepared from the following components: phosphate buffer, 1.8-2.2mL / L Triton X-100, 0.05-0.10g / EDTA dihydrate disodium salt in L, ProcLin300 in 0.8-1.2mL / L, cystathionine-β-synthase in 20.0-22.0KU / L and cystathionine-β-synthase in 10.0-14.0KU / L ‑Decomposing enzymes. The homocysteine ​​determination kit forms a reagent R1 and a reagent R2 by screening each component containing a specific ratio, and has a low detection limit, high sensitivity, wide linear range as a whole, and good stability.

Description

Technical field [0001] The present invention relates to the field of biological detection, and more particularly to a homocityycysocysteine ​​determination kit and a method of preparing a method and a detection method thereof. Background technique [0002] Homolysteine, Hcy) is also known as high cysteine, is a hygrodionic amino acid, a white crystalline, melting point 232 ° C to 233 ° C, can be dissolved in water and acetic acid, slightly soluble in alcohol, insoluble The ether is the intermediate product formed in the cystylethyl ether and the proteinic acid to vulcanize or the methylation metabolic bycoming, is an amino acid precursor that is not normalized during the protein biosynthesis. Hcy in the main two metabolic processes of the human body, namely the methylation process and the thigenliosis process. The methylation process: Hcy can re-formulate methionine reforming methionine and participate in methionine cycles, this process requires vitamin B12, methyltransferase, me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 李海军陶成刚李建奎黄锴陈肯李小玉
Owner WUHAN CHANGXINSHENG BIOTECH
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