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Microplate thermal shift assay for ligand development and multi-variable protein chemistry optimization

The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change. The method comprises contacting the target molecule with one molecule of the multiplicity of different molecules in each of a multiplicity of containers, simultaneously heating the multiplicity of containers, measuring in each of the containers a physical change associated with the thermal denaturation of the target molecule resulting from the heating in each of the containers, generating a thermal denaturation curve for the target molecule as a function of temperature for each of the containers and determining a midpoint temperature (T.sub.m) therefrom, comparing the T.sub.m of each of the thermal denaturation curves with the T.sub.m of a thermal denaturation curve obtained for the target molecule in the absence of any of the molecules in the multiplicity of different molecules, and ranking the affinities of the multiplicity of different molecules according the change in T.sub.m of each of the thermal denaturation curves.
Owner:LIFE TECH CORP

Apparatus and method for compound profiling of living cells

An apparatus and method for real-time measurement of a cellular response of a test compound or series of test compounds (303) on a flowing suspension of cells (349), in which a homogeneous suspension of each member of a series of cell types (349) is combined with a concentration of a test compound (303), directed through a detection zone (355), and a cellular response of the living cells is measured in real time as the cells in the test mixture are flowing through the detection zone (355). The apparatus may be used in automated screening of libraries of compounds, and is capable of real-time variation of concentrations of test and standard compounds and generation of dose / response profiles within a short timespan.
Owner:CAPLIPER LIFE SCI INC

Digital audio signal filtering mechanism and method

Essentially all of the processing parameters which control processing of a source audio signal to produce an encoded audio signal are stored in an audio processing profile. Multiple audio processing profiles are stored in a processing profile database such that specific combinations of processing parameters can be retrieved and used at a later time. Audio processing profiles are organized according to specific delivery bandwidths such that a sound engineer can quickly and efficiently encode audio signals for each of a number of distinct delivery media. Synchronized A / B switching during playback of various encoded audio signals allows the sound engineer to detect nuances in the sound characteristics of the various encoded audio signals.
Owner:MICROSOFT TECH LICENSING LLC

Cell flow apparatus and method for real-time measurements of cellular responses

An apparatus and method for real-time measurement of a cellular response of a test compound or series of test compounds (303) on a flowing suspension of cells (349), in which a homogeneous suspension of each member of a series of cell types (349) is combined with a concentration of a test compound (303), directed through a detection zone (355), and a cellular response of the living cells is measured in real time as the cells in the test mixture are flowing through the detection zone (355). The apparatus may be used in automated screening of libraries of compounds, and is capable of real-time variation of concentrations of test and standard compounds and generation of dose / response profiles within a short time span.
Owner:CAPLIPER LIFE SCI INC

Microanalysis of cellular function

ActiveUS20130261021A1Rapid and efficient testingRapid and efficient timingDielectrophoresisLibrary screeningAntibody-Secreting CellsGranular cell
An inverted microwell (102) provides rapid and efficient microanalysis system (100) and method for screening of biological particles (128), particularly functional analysis of cells on a single cell basis. The use of an inverted open microwell system (102) permits identification of particles, cells, and biomolecules that may be combined to produce a desired functional effect also functional screening of secreted antibody therapeutic activity as well as the potential to recover cells and fluid, and optionally expand cells, such as antibody secreting cells, within the same microwell.
Owner:CELLPLY SRL

Technological process for producing high concentration nitric-phosphate fertilizer

The invention discloses a technique method for producing high-concentration nitric phosphate, which comprises the following steps of: adding nitric acid into phosphorus ore for acidolysis, depositing and separating acid non-soluble substance, freezing and crystallizing calcium nitrate, filtering the calcium nitrate, neutralizing the mother liquid, vaporization, pelleting and drying. The invention is characterized in that: a step of thoroughly removing the calcium of the mother liquid is arranged between the steps of filtering the calcium nitrate, and neutralizing the mother liquid. The steps comprise the following steps of: I) thoroughly removing the calcium of the mother liquid and adding sulfuric acid or ammonium sulfate; the calcium ions and the sulfate ions in the mother liquid generate dihydrate calcium sulfate crystal; II) the grading processing of reaction slurry: employing a grading device to carry out grading processing to the reaction slurry; returning the reaction slurry provided with small grain calcium sulfate to the mother liquid for thoroughly removing the calcium, and feeding the reaction slurry provided with large grain calcium sulfate to the calcium sulfate for filtering; and III) filtering and washing the filtering reaction slurry of calcium sulfate by a filter, feeding the primary filtrate and the primary lavage fluid into the working procedure of neutralizing, and returning the secondary lavage fluid to the filter. The invention has the advantage of using middle-low quality phosphorite to prepare the high-concentration nitric phosphate.
Owner:TIANJI COAL CHEM IND GROUP +1

Device and method of detecting mutations and polymorphisms in DNA

There is provided a resonator sensor useful for detecting polymorphisms and mutations in DNA. The resonator sensor has a capture molecule immobilised on its surface, the capture molecule being either a probe DNA containing a reference sequence, or a mismatch binding molecule, and being capable of forming a probe DNA / target DNA / mismatch binding molecule complex on the surface of the resonator. A method for detecting mutations in a target DNA, including single nucleotide polymorphisms, is also provided.
Owner:AGENCY FOR SCI TECH & RES +1

Novel adherence factors of non pathogenic microorganisms and applications thereof for screening microorganisms for specific probiotic properties; novel pharmaceutical compositions and food additives comprising such microorganisms and adherence factors

InactiveUS20010018048A1Rapid screeningQuick filterBiocideBacteriaDiseaseSAA protein
A protein obtainable from a non pathgenic microorgansim, said protein having mucosa binding promoting activity and a molecular weight of 20-40 kD is disclosed. Application of such a protein or a peptide derived therefrom in a method of screening non pathogenic microorganisms for a microorganism capable of specifically binding mucosa, said method comprising detection in a manner known per se of the presence of a particular protein on or in a microorganism or in a culture of microorganisms, said particular protein being the already defined protein. Kits suitable for such a screening method are also disclosed. Use of a component selected from the group of components comprising a protein or peptide as defined; an expression vector comprising nucleic acid encoding such protein or peptide; a recombinant microorganism or a part of said microorganism expressing such protein or peptide, said part expressing mucosa binding promoting activity; a non pathogenic microorganism capable of expressing such protein or peptide or a part of said microorganism, said part expressing mucosa binding promoting activity as pharmaceutically active component in a pharmaceutical composition for prophylaxis and / or treatment of disease or illness associated with a mucosa colonizing pathogenic microorganism. Use of such components as food additive and compositions comprising such components are described.
Owner:NEDERLANDSE ORG VOOR TOEGEPAST-NATUURWETENSCHAPPELIJK ONDERZOEK (TNO)

Method and architecture for improving defect detectability, coupling area, and flexibility of nvsram cells and arrays

Several preferred embodiments of 1S1F 16T NVSRAM, 1S1F 20T NVSRAM, 1S2F 22T NVSRAM, 1S2F 14T NVSRAM cells are proposed, regardless of 1-poly, 2-poly, PMOS or NOS flash cell structures. Two separate sourcelines for the paired flash Strings are also proposed for easy adding ability for the NVSRAM circuit to detect the marginally erased Vt0 and marginally programmed Vt1 of the paired flash cell. By increasing an resistance added to common SRAM power line, the pull-down current through flash Strings to grounding source line can be made much larger than the pull-up current to improve SFwrite program operation. Simple method by increasing flash cell channel length to effectively enhance coupling area is applied to secure SRAM-to-Flash store operation under self-boost-program-inhibit scheme. 1S2F architecture also provide flexibility for alternate erasing and programming during both a recall and store operation.
Owner:APLUS FLASH TECH

Quick multi-keyword semantic sorting search method for protecting data privacy in cloud computing

The invention relates to a quick multi-keyword semantic sorting search method for protecting data privacy in cloud computing. A domain weighted scoring concept is introduced in document scoring, and keywords in different domains such as a title, an abstract and the like are endowed with different weights to be distinguished; a retrieval keyword is subjected to semantic expansion, semantic similarity is calculated, a three-factor sorting method is designed by combining the semantic similarity, the domain weighted scoring and a correlation score, and a cloud server can perform accurate sorting on search results and return a sorting result to a search user; and for the defect of low query efficiency of a searchable encryption scheme, a vector block segmentation marking matching algorithm is designed, and a document vector created by a vector space model is subjected to block segmentation to generate a marking vector with a relatively small dimension number. According to the method, the query efficiency can be improved, the index creation time can be shortened, and semantic ciphertext keyword search is realized.
Owner:FUZHOU UNIV

Methods & Compositions for Selection of Loci for Trait Performance & Expression

The present invention provides novel methods and compositions for the identification and selection of loci modulating transgene performance and expression in plant breeding. In addition, methods are provided for screening germplasm entries for the performance and expression of at least one transgene.
Owner:MONSANTO TECH LLC

Cluster-based real-time rendering service of remote sensing data set

InactiveCN102722549AImplement multi-mode parallel computing featuresAvoid congestionResource allocation2D-image generationSensing dataTerrain
The invention provides a systemic solution aiming at the rapid clustered rendering of a multisource remote sensing data set, which is especially used for solving distributed organization and storage problems of data and union query problems of a plurality of data sets in mass remote sensing data and different kinds of data sets. The systemic solution comprises the following steps: firstly allocating a cluster environment suitable for a real-time mass remote sensing data rendering system, wherein the cluster environment is formed by a plurality of calculation and data integrating nodes and a central server; sending a tile rendering request of a client side to the central server through a network channel, carrying out the research, the sieving and the post-processing through the central server so as to obtain a visible data list within the tile scope, and determining the optimal node according to the storage position; reading the tiles corresponding to the data by the nodes, rendering the tiles to a canvas in an overlying mode one by one, and sending the results to the central server; and finally sending the results to the client side by the central server. The systemic solution provided by the invention is suitable for rendered grain on the two-dimensional or three-dimensional surfaces, and large scale application fields, such as visualization and the like in the production of the terrain tile production and the large-scale remote sensing data management.
Owner:REMOTE SENSING APPLIED INST CHINESE ACAD OF SCI
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