45 results about "Sex specific" patented technology

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a HEPES bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

The invention relates to a method and apparatus for pre-hatch avian embryo sex determination. In particular, the invention relates to a non-invasive method and apparatus for in-ovo determining the sex of avian species while in the egg and allowing to sort the eggs into groups consisting primarily of either male or female embryos. The method comprises the steps of introducing into the egg an antibody designed to match with a sex specific antigen on the embryo, which antibody is labelled, allowing the labelled antibody to migrate to and bind with the sex specific antigen on the embryo, detecting binding of the labelled antibodies on the embryo using detection means positioned outside of the egg.

The invention relates to methods for determining the sex of birds' eggs. It can be used for an early determination of the sex, in particular in poultry farming, and in this respect also with non-incubated eggs. In the method in accordance with the invention, electromagnetic radiation is emitted onto the blastodisk of an egg by a radiation source and, after a switching off of the radiation source, the decay behavior of the autofluorescence intensity excited by the electromagnetic radiation is detected by a detector at the irradiated region of the blastodisk with time resolution and spectral resolution for at least one wavelength of the autofluorescence. The fractal dimension DF is calculated using the determined measured intensity values and the value of the fractal dimension DF is compared with a species-specific and sex-specific limit value, wherein, when the limit value is exceeded, the respective egg is classified as female and, when it is not reached, the egg is classified as male.

The present invention is directed to a panel of single nucleotide polymorphisms (SNPs) in specific genes that serve as biomarkers for sex-specific childhood leukemia risk. There is provided herein methods and reagents for assessing the specific SNPs in those genes. The method useful in applying these SNPs in predicting an increased risk or a decreased risk for childhood leukemia for males and females is also disclosed.

The invention relates to spotted halibut sex-specific molecular markers and a genetic sex detection method. The method comprises the following steps of extraction of fin ray DNA, AFLP analysis of DNA of genomes, screening and sequencing of the sex-specific AFLP markers, and establishment of the genetic sex identification method. The genetic sex detection method establishes the spotted halibut fin ray DNA extraction method and the AFLP analysis technology, screens out two female-specific AFLP molecular markers the molecular weight of which is respectively 533 bp and 218 bp, obtains nucleotide sequences of the two AFLP markers, and establishes the molecule marking technology of spotted halibut sex-specific identification. The method has the advanced, accurate and reliable characteristics, has important application value in control of spotted halibut sex and production of full-female seedlings, and simultaneously has wide application prospect in genetic sex identification and sex control and research of other fish varieties.

The invention discloses a PCR primer pair used for identifying the genetic sex of Pseudobagrus ussuriensis, and a rapid identification method thereof. The identification method comprises the steps of examined individual tissue drawing, genome DNA extraction, sex specific DNA fragment PCR amplification, agarose gel electrophoresis, ultraviolet light detection shooting and examined individual genetic sex determination. High-quality genome DNA is extracted through an optimized process, a sex specific DNA fragment is amplified, and the existence or not and the existence characteristic of the sex specific fragment are detected under ultraviolet lights, so the genetic constitution of Pseudobagrus ussuriensis is identified, and the genetic sex is discriminated in 1-2d. The PCR primer pair and the rapid identification method provide a rapid identification method for identifying sex reversal fish and super-male fish, provide technical support for breeding all-male fish, and have the advantages of breeding time shortening, great increase of the output, breeding cost reduction, and profound economic and social values.

The invention relates to methods for determining the sex of birds' eggs. It can be used for an early determination of the sex, in particular in poultry farming, and in this respect also with non-incubated eggs. In the method in accordance with the invention, electromagnetic radiation is emitted onto the blastodisk of an egg by a radiation source and, after a switching off of the radiation source, the decay behavior of the autofluorescence intensity excited by the electromagnetic radiation is detected by a detector at the irradiated region of the blastodisk with time resolution and spectral resolution for at least one wavelength of the autofluorescence. The fractal dimension DF is calculated using the determined measured intensity values and the value of the fractal dimension DF is compared with a species-specific and sex-specific limit value, wherein, when the limit value is exceeded, the respective egg is classified as female and, when it is not reached, the egg is classified as male.

Current invention is about the sex-specific separation of sperm in high purity. For more details, this invention is about the methods for improving the separation efficiency of sperm by removing factors deteriorating separation efficiency by utilizing several parameters and adjusting few conditions for the purpose of separating sperm into X-chromosome bearing and Y-chromosome bearing groups in high purity. Current invention, for the purpose of separating X-chromosome bearing and Y-chromosome bearing sperm based on physiological characteristics, utilize the difference of sperm's nucleus width as a parameter to recognize the difference in DNA content between X and Y chromosome. Current invention is about the sex-specific separation of sperm in high purity. This invention, unlike any known conventional methods, requires neither special conditions nor special treatments, can be conducted in a more realistic environment and, as a results, sex-specific sorting of sperm can be conducted without losing viability or motility. Therefore, the limitation conferred on sorting efficiency by the motility can be overcome by our invention.

The invention provides a striped beakfish sex specific molecular marker and a genetic sex identification method thereof. The method comprises: screening and cloning of a male specific amplified fragment length polymorphism (AFLP) marker; performing DNA sequence analysis on sex specificity; and constructing a genetic sex polymerase chain reaction (PCR) identification method. In the invention, 4 primer combinations selected from 144 pairs of primer combinations can produce 4 male specific AFLP markers; the AFLP markers are cloned and sequenced to screen out a sequence characterized amplified regions (SCAR) marker molecular marker; and thus, the genetic sex identification method for striped beakfish is established. In the invention, the AFLP method is used for the first time to screen out the sex specific molecular marker for the striped beakfish and convert the sex specific molecular marker for the striped beakfish into the SCAR marker so as to establish the genetic sex identification method for the striped beakfish. The striped beakfish genetic sex identification method has the advantages of accuracy, sensitivity, reliability and the like, has high scientific value for the study onfish sex and has a great significance and high application value for the sex control and selective breeding of fish.

The invention belongs to the field of fish sex identification in the field of aquaculture, and particularly relates to a specific DNA fragment for hemibagrus wyckioides sex determination and application. On the basis of combining a low coverage whole genome survey technology with a 2b-RAD-seq technology, and by means of a genome walking technology, hemibagrus wyckioides male sex specific markerisdeveloped, the specific DNA fragment is shown in SEQIDNO.2 or SEQIDNO.1. For the specific sequence, the sex of hemibagrus wyckioides can be identified by means of conventional PCR amplification, and it is verified thatthe accuracy rate reaches 100%. Compared with previous anatomical detection or genital swelling observation, thetechnology is accurate, simple, rapid, less in harmful to fish body and the like, and help is provided for hemibagrus wyckioides sex control breeding, wild resource protection and the development of the aquaculture industry.

The invention discloses a trachinotus ovatus sex specific molecular marker primer. The primer comprises a forward primer and a reverse primer, wherein a base sequence of the forward primer is as shownin SEQ ID NO: 1, and a base sequence of the reverse primer is as shown in SEQ ID NO: 2. The invention also discloses a method for identifying male and female of trachinotus ovatus by using the aboveprimer, and an application of the above primer or method in preparing a kit or biological preparation for identifying male and female of trachinotus ovatus. The primer has good specificity, and the identification method is convenient to operate, is simple and easy to implement, is high in identification success rate, does not harm the live body of trachinotus ovatus, can quickly and accurately identify the sex of trachinotus ovatus in batches at once, and has important scientific research value and practical application value.

The present invention relates to populations of male and female non-human animals which can be induced to produce single sex populations, and which may comprise a heterologous gene of interest, preferably a neurodegenerative disease gene, which is expressed in a tissue specific manner. The invention also relates to methods for sorting a mixed population of non-human animal embryos and / or larvae into a single sex population.

The invention relates to a method for rapidly identifying a charybdis feriata sex specific molecular marker and genetic sex. For SNP sites used for charybdis feriata sex identification, the base sequence is SEQ ID NO:1 or SEQ ID NO:2, and the length is 368bp; the SNP sites are the 35 site, the 89 site, the 120 site, the 218 site and the 240 site of SEQ ID NO:1 or SEQ ID NO:2. The identification method comprises the steps that charybdis feriata male and female individuals with known sex is subjected to DNA extraction; genomic library building and high throughput sequencing are simplified; sequence analysis and sex specific SNP site screening are conducted; candidate SNP site enlarged sample validation is conducted; sex specific primers based on the sex specific SNP sitesare designed; by means of the male specific primers, under specific annealing temperature, the genome DNA of charybdis feriata is subjected to PCR amplification, and specific sex determination is conducted according to the agarose gel electrophoresis detection result. A reliable method can be provided for charybdis feriata genetic sex and especially for the charybdis feriata in early developmentstage, and the method has the advantages of being easy to operate, short in consumed time, low in cost, good in repetition, high in accuracy rate and the like.

The invention belongs to the technical field of colony identification in aquaculture, and discloses a micro-satellite marker and specific primer for identifying female and male individuals of pseudobagrus ussuriensis, and application. One sex-specific micro-satellite site of the pseudobagrus ussuriensis is provided, and has a nucleotide sequence shown by any one of SEQ ID NO:1-2. The invention also provides a primer designed from the micro-satellite site, and the primer has a nucleotide sequence shown by SEQ ID NO:3-4. One micro-satellite site is screened out of a micro-satellite enriched library of the pseudobagrus ussuriensis, and a specific primer is designed according to flanking sequences at two ends of a micro-satellite, so that an amplification result has sex specificity, the female and male individuals of the pseudobagrus ussuriensis can be effectively identified, and the specific primer can also be used for performing real-time monitoring on sex structures of colonies in natural water areas and breeding places. The invention also discloses a kit for identifying the female and male individuals of the pseudobagrus ussuriensis. The identification method provided by the invention has the advantages of high accuracy, high resolution ratio and high speed.

A PCR-based rapid genetic sex identification method for Scylla Paramamosain is disclosed. The method includes (1) designing control primers, and designing female-specific primers by utilizing a base mismatch process; (2) extracting genome DNA of a Scylla Paramamosain individual to be tested; (3) by using the control primers and female-specific primers respectively, and by using the genome DNA of Scylla Paramamosain as a template, performing PCR amplification at an annealing temperature of 65 DEG C to obtain corresponding PCR products; and (4) detecting the PCR products through electrophoresiswith agarose gel having a concentration of 1.5% to determine whether the individual is male or female. According to the method, the base mismatch process of the sex-specific SNP site is utilized to design the female-specific primers, amplification is performed through an optimized PCR reaction system and a PCR reaction program, and then electrophoresis with agarose gel is performed for detection to achieve genetic sex identification of the Scylla Paramamosain. The method has advantages of simple operation, a high accuracy rate, a low cost, good repeatability and high practicability, and can beused for large-scale rapid identification for Scylla Paramamosain in the early period of growth and development.

A method for influencing sex selection in artificial insemination, the method characterised by the exposure of unsexed semen to antibodies to sex specific antigens present on spermatozoa carrying chromosomes of an undesired sex, whereby the spermatozoa to which the sex specific antigens are bound are generally unable to effect fertilisation of an egg, such that fertilisation is likely to be effected by spermatozoa carrying chromosomes of a desired sex.

The invention relates to a method and apparatus for pre-hatch avian embryo sex determination. In particular, the invention relates to a non-invasive method and apparatus for in-ovo determining the sex of avian species while in the egg and allowing to sort the eggs into groups consisting primarily of either male or female embryos. The method comprises the steps of introducing into the egg an antibody designed to match with a sex specific antigen on the embryo, which antibody is labelled, allowing the labelled antibody to migrate to and bind with the sex specific antigen on the embryo, detecting binding of the labelled antibodies on the embryo using detection means positioned outside of the egg.

The invention provides kiwi fruit sex specific molecular marker primers based on a fluorescent capillary electrophoresis detection technology and application thereof, and belongs to the technical field of molecular biology. According to the specific molecular marker primers, three loci can be amplified in the same PCR reaction system, the three loci comprise two male specific loci and one genomic DNA internal reference ITS locus, and the three loci are simultaneously detected in fluorescent capillary electrophoresis detection, so that the reliability of a detection system is greatly improved, and the detection cost is reduced.

The invention discloses a specificity detection primer, a detection kit and a detection method for schistosoma japonicam sex, an upstream primer Tsexu2 nucleotide sequence of the specificity primer is 5'-ACGTTAGATACTGCTGTTCA-3, a downstream primer Tsexd2 nucleotide sequence is 5-ATATTGTTCCAAGTACGCAT-3'. In the invention, PCR amplification is carried out on the mould DNA to be detected by the specificity detection primer, the agarose gel electrophoresis is carried out on the amplifying product which is then observed under the ultraviolet light, the amplification strip appears in the positive result, which does not appear in the negative result. In the invention, the specificity detection primer is designed according to female worm specific sequence data of the schistosoma japonicam to establish a rapid, specific, sensitive PCR method used for schistosoma japonicam epidemiology and sex identification. The kit of the invention has simple procedures, strong specificity of the method, high sensitivity and objective result determination, and can be used for sex specific diagnosis of schistosoma japonicam of human and animals.

The invention provides a patinopecten yessoensis living body sex identification method based on DNA molecular markers. The patinopecten yessoensis living body sex identification method based on the DNA molecular markers comprises the steps that a small amount of gill filaments is obtained from the patinopecten yessoensis through a living body, DNA is extracted, PCR amplifies the sex-specific DNA molecular markers, whether a target band exists in a PCR product or not is detected by electrophoresis, and patinopecten yessoensis living body sex identification is achieved. The sex-specific DNA molecular markers are unique to female, and a nucleotide fragment sequence of the sex-specific DNA molecular markers is SEQ ID NO:1. PCR primers are used for amplifying the molecular markers, and the sexof patinopecten yessoensis is identified based on the agarose gel electrophoresis result; and a sample amplifying a 1859bp specific band is female, and samples without the band are male. The patinopecten yessoensis living body sex identification can be realized, the method is simple, the result is accurate, and the method is helpful to speed up the breeding process of the patinopecten yessoensis.

The invention relates to spotted halibut sex-specific molecular markers and a genetic sex detection method. The method comprises the following steps of extraction of fin ray DNA, AFLP analysis of DNA of genomes, screening and sequencing of the sex-specific AFLP markers, and establishment of the genetic sex identification method. The genetic sex detection method establishes the spotted halibut finray DNA extraction method and the AFLP analysis technology, screens out two female-specific AFLP molecular markers the molecular weight of which is respectively 533 bp and 218 bp, obtains nucleotide sequences of the two AFLP markers, and establishes the molecule marking technology of spotted halibut sex-specific identification. The method has the advanced, accurate and reliable characteristics, has important application value in control of spotted halibut sex and production of full-female seedlings, and simultaneously has wide application prospect in genetic sex identification and sex controland research of other fish varieties.

The invention provides compositions and methods for determining the likelihood of gender-specific successful treatment with 5-FU / oxaliplatin or an equivalent of each thereof. The methods comprise determining the genomic polymorphism present in a predetermined region of a gene of interest and correlating the polymorphism to the predictive response. Patients identified as responsive are then treated with the appropriate therapy.

The present invention relates to markers for diagnosing the difference in effects of renal cancer treatment or the prognosis of renal cancer patients, according to the gender of renal cancer patients. The survival rate and recurrence rate of renal cancer of a particular gender respectively relate to the mutation of genes, of the present invention, in renal cancer patients, and thus the mutated genes of the present invention can be used as markers in predicting, on the basis of gender, the difference in effects of renal cancer treatment or the prognosis of renal cancer patients.

Provided are an antibody for sexing sperms and use thereof, and more particularly, a composition for and a method of sexing sperms by using the antibody, and a method of producing an animal of a particular sex. In the present disclosure, it was confirmed that agglutination of Y chromosome sperms may be induced by treatment of the antibody, thereby easily sorting X chromosome sperms and Y chromosome sperms. Therefore, it is possible to produce a large number of customized animals of a particular sex and to selectively produce livestock of a desired sex, and therefore, it is expected to contribute to planed breeding, breeding improvement, and efficient management.

The invention discloses a leiocassis longirostris male sex specific molecular marker and an amplification primer and a genetic sex identification method thereof. The nucleotide sequence of the leiocassis longirostris male specific molecular marker is as shown in SEQ ID No. 1, and the nucleotide sequences of the amplification primers of the leiocassis longirostris male specific molecular marker are as shown in SEQ ID No. 2 and SEQ ID No. 3. The leiocassis longirostris genomic DNA is subjected to PCR amplification by using the amplification primers, the product is subjected to electrophoresis detection, if a specific band is amplified, the leiocassis longirostris is identified as a male individual, and if a specific target band is not amplified, the leiocassis longirostris is identified as a female individual, and the genetic sex identification method is simple, rapid, direct, high in accuracy rate, small in damage to the leiocassis longirostris, the method is not limited by the development period of the leiocassis longirostris, solves the problem of difficulty in sex identification in the early development period of the leiocassis longirostris, and has important significance for realizing sex control breeding of the leiocassis longirostris in production.

The invention provides a development method and the application of a siniperca chuatsi sex specific marker. The development method comprises the following steps: (1) obtaining male and female siniperca chuatsi specific short sequences as shown in SEQ ID NO.1 by utilizing a high-throughput sequencing method based on a 2b RAD technology; (2) obtaining a male siniperca chuatsi whole genome sequence by using a high-throughput de novo sequencing method; and (3) searching the male siniperca chuatsi specific short sequence obtained in the step (1) in the male siniperca chuatsi whole genome sequence obtained in the step (2) to obtain a longer male siniperca chuatsi specific sequence, namely SEQ ID NO.2, and performing later primer design to be applied to genetic sex identification of siniperca chuatsi. The method has the advantages of quickness, directness, simplicity, convenience and the like, and sex molecular identification can be performed on a large number of samples.

The invention provides a striped beakfish sex specific molecular marker and a genetic sex identification method thereof. The method comprises: screening and cloning of a male specific amplified fragment length polymorphism (AFLP) marker; performing DNA sequence analysis on sex specificity; and constructing a genetic sex polymerase chain reaction (PCR) identification method. In the invention, 4 primer combinations selected from 144 pairs of primer combinations can produce 4 male specific AFLP markers; the AFLP markers are cloned and sequenced to screen out a sequence characterized amplified regions (SCAR) marker molecular marker; and thus, the genetic sex identification method for striped beakfish is established. In the invention, the AFLP method is used for the first time to screen out the sex specific molecular marker for the striped beakfish and convert the sex specific molecular marker for the striped beakfish into the SCAR marker so as to establish the genetic sex identification method for the striped beakfish. The striped beakfish genetic sex identification method has the advantages of accuracy, sensitivity, reliability and the like, has high scientific value for the study onfish sex and has a great significance and high application value for the sex control and selective breeding of fish.

The invention relates to the technical field of molecular biology, and discloses a molecular marker and a detection method for quickly identifying true and false male fish of cynoglossus semilaevis based on RAA-LFD. The method comprises the following steps: S1, selecting a ZW chromosome homologous allele sequence and searching a candidate SNP site; S2, designing a primer pair of an allele sequencecontaining the candidate SNP site by using Primer 3; and S3, carrying out first-generation sequencing on the amplification product of the step S2, and determining a sex specific site through verification of the first-generation sequencing. A new rapid detection method for sex identification is provided, and meanwhile, possibility is provided for on-site timely detection. Generally, laboratory workers or farm farmers only need to set an RAA amplification reaction system and distribute the RAA amplification reaction system into a small centrifuge tube. In the detection process, only sample DNAis added, the product amplification condition can be detected through LFD after incubation is conducted for 40 minutes at the temperature of 39 DEG C, and the detection method has the advantages of being easy and rapid to operate, high in specificity and sensitivity, convenient to detect and the like.

The invention belongs to the field of fish sex identification in the field of aquaculture, and particularly relates to a specific DNA fragment for hemibagrus wyckioides sex determination and application. On the basis of combining a low coverage whole genome survey technology with a 2b-RAD-seq technology, and by means of a genome walking technology, hemibagrus wyckioides male sex specific markerisdeveloped, the specific DNA fragment is shown in SEQIDNO.2 or SEQIDNO.1. For the specific sequence, the sex of hemibagrus wyckioides can be identified by means of conventional PCR amplification, and it is verified thatthe accuracy rate reaches 100%. Compared with previous anatomical detection or genital swelling observation, thetechnology is accurate, simple, rapid, less in harmful to fish body and the like, and help is provided for hemibagrus wyckioides sex control breeding, wild resource protection and the development of the aquaculture industry.