8 results about "Genomic library" patented technology

[Problem] To provide: an adapter for binding to polynucleotide fragments that is used for preparing a genome-wide gRNA library for genome editing systems; a gRNA library that is obtained by using the adapter; and uses of the gRNA library. [Solution] An adapter having a structure described in A). A) A structure having i) to iii). i) A polynucleotide chain having sequences i)-1 to i)-3. i)-1: Sequence corresponding to the forward primer. i)-2: Sequence of the recognition site of a restriction enzyme capable of excising a gRNA spacer sequence from the 5' end of a polynucleotide fragment bound to the adapter. i)-3: Sequence of the recognition site of a restriction enzyme capable of cleaving an adapter having the structure of A) from the polynucleotide fragment. ii) A polynucleotide chain having the sequence complementary to i). iii) A single-stranded polynucleotide chain for formation of a loop connecting the 5' end of i) and the 3' end of ii) of the double strand resulting from the binding of i) and ii).

The application belongs to the field of molecular biology and biotechnology, and particularly relates to a probe combination for capturing a mammalian mitochondrial genome and application thereof. The nucleotide sequences of the probe combination are the sequences shown in SEQ ID NO: 1~24537 in turn. The probe combination has good capture efficiency on the mitochondrial genome of mammals, and can obtain a complete mitochondrial genome sequence. Moreover, the probe combination has very high sensitivity, and for low-concentration DNA and highly degraded difficult samples, more than 90% of the samples can still capture the target sequence. The application can mix 4~16 mammalian sample fragmented whole genome libraries, capture, sequence and splice by using the probe combination, obtain the corresponding mitochondrial genome sequence, reduce the cost of obtaining the mtDNA complete sequence of a single sample by 70~75%, and greatly improve the feasibility of large sample quantity research.

A system for producing a therapeutic oligomer includes a computing device configured to design a proposed therapeutic oligomer sequence, wherein designing further comprises generating a genomic library for an organism from a gene target, initiating a sequence identification function, identifying a genomic locus that the proposed therapeutic oligomer sequence is predicted to bond to as a function of an off-target sequence function, selecting the proposed therapeutic oligomer sequence as a function of the sequence identification function, the genomic locus, and a criterion element, and synthesize a therapeutic oligomer as a function of the proposed therapeutic oligomer sequence.

The invention discloses a screening method for targeted zinc amino acid chelate metabolism genes based on a Crispr whole genome library. The method comprises the following steps: constructing a zinc transporter gene knockout intestinal epithelial cell single gene knockout cell strain; transfecting the Crispr whole genome knockout library into an intestinal epithelial cell single gene knockout cell strain to obtain an intestinal epithelial cell double gene knockout cell strain; treating the intestinal epithelial cell double-gene knockout cell strain with different zinc sources, dyeing with a fluorescent dye, and sorting zinc transport difference intestinal epithelial cell double-gene knockout cell strains through flow cytometry; the separated cell lines are subjected to gDNA extraction, gRNA sequence amplification and high-throughput sequencing, and key target genes for regulating and controlling metabolism of different amino acid chelated zinc are analyzed and identified through bioinformatics. The invention establishes a method for high-throughput screening of the specific metabolism gene of the amino acid chelated zinc, and a Zn metabolism regulation gene library is also obtained through screening at the same time.

The application is particularly a method for automatically annotating a data set of a cytological pathology deep learning model, comprising the following parts: step one: using a single cell micro-operation device to automatically locate and suck cells on a glass sheet dyed by cytological pathology; step two: using a fully automated liquid processing workstation to construct a single cell whole genome library and sequence the extracted cells; step three: determining the benignity and malignancy of the cells through analysis of the single cell whole genome sequencing data, so as to construct a training data set of the cell dyeing picture after sequencing for training a deep learning model. In the application, combined with scDNA-Seq and automatic robot technology for single cell and liquid operation, the ETC annotation method guided by scDNA-Seq provides a standard and scalable method for automatic ETC annotation, and represents a general method for developing a deep learning model for pathological diagnosis of malignant tumor cells in different types of body fluids.

The application discloses a single-cell exon sequencing method and application thereof, and the method comprises the following steps: S1, tumor single-cell sorting; S2, MDA amplification of the sorted tumor single cells to obtain a genomic library; S3, detection of MDA amplification product uniformity, selection of multiple sites located on different chromosomes and design of corresponding amplification primers, detection of amplification effects of the multiple sites of each single-cell MDA product by qPCR, and determination of whether the genome is uniformly amplified; S4, gDNA library preparation: the amplified DNA is broken into 150-200bp fragments, and a gDNA library is obtained through end repair, tailing, adapter addition and amplification; S5, exon capture and library construction: the prepared gDNA library is hybridized with specific probes, specific regions are captured, and an exon library is obtained by PCR amplification and addition of index tags; and S6, sequencing of the exon library.

Provided herein are compositions and methods for standardizing a sequencing library. Also provided herein are adapter conjugates and hybridized cyclic adapters, compositions comprising the same, and methods of producing the same. Also provided herein are methods of standardizing genomic DNA libraries for next generation sequencing using the adapter conjugates, hybrid circular adapters, and compositions comprising them.